Liquid collection container and liquid component extraction system

Abstract

Provided is a liquid collection container that makes it possible to extract a collected liquid in a closed sate. A liquid collection container A1 includes: a container body 1 having a cylindrical part 11 extending in a first direction x; a gasket 2 forming a first space S1 inside the container body 1 by closely adhering to the inner peripheral surface of the cylindrical part 11 without a gap in the circumferential direction; a closing wall 12 that is disposed at the x1 end on one side in the first direction of the first cylindrical part 11 and that is capable of switching between a state in which the first space S1 is in communication with the outside and a state in which the first space S1 does not communicate with the outside, first plug parts 3, and flow path tubes 4 (first switching part); and a plunger 5 that is connected to the gasket 2, that extends from an opening 111 at the x2 end on the other side in the first direction of the first cylindrical part 11 toward the other side in the first direction x2, and that is capable of imparting a force for sliding the gasket 2 in the first direction x. The closing wall 12 closes the x1 end on the one side in the first direction of the first cylindrical part 11 and has communication holes 121 passing therethrough in the first direction x. The first plug part 3 is equipped with a first connection part 31 that has gas impermeability and liquid tightness. The flow path tubes 4 each have a first end part 41 that is connected to the closing wall 12 in a state of communicating with the communication hole 121 and a second end part 42 that is closed at the first connection part 31.

Description

[Technical Field]

[0001] The present disclosure relates to a liquid collection container for collecting a liquid, and a liquid component collection system including the liquid collection container. [Background technology]

[0002] Containers known as vacuum blood collection tubes are widely used to collect blood for testing. Vacuum blood collection tubes consist of a main body made of a conical bottom wall formed from plastic or glass and a cylindrical side wall rising from the periphery of the bottom wall, and a rubber stopper that closes the opening of the main body (see, for example, Patent Document 1). The stopper may be made of an aluminum-containing sheet, with only the needle insertion portion being rubberized. A reduced pressure state is maintained inside the vacuum blood collection tube, and blood can be introduced into another container or directly from a vein in the body by inserting a needle into the rubber portion of the stopper.

[0003] Standard vacuum blood collection tubes like the one above are primarily used for blood testing, and in most cases, test results are obtained by mixing the blood that has been poured into the built-in reagent. If the collected blood needs to be transferred to another container, the stopper is opened and the blood is aspirated using a tool.

[0004] In particular, when the goal is to use a portion of blood components as a source of iPS cells for regenerative medicine, a vacuum blood collection tube containing a whole blood sample is centrifuged to separate the blood components, and only the necessary components are aspirated and collected. However, the main body of a vacuum blood collection tube is made of a hard material, due to its structure that maintains a reduced pressure until the blood collection process. Therefore, even when aspirating from a tube connected by inserting a needle into the stopper, pressure resistance makes it difficult to collect the contained liquid. Therefore, at least part of the stopper must be opened and aspirated using an instrument. However, this requires high-level aseptic equipment and specialized skills to ensure the sterility of the raw material, and the high equipment maintenance costs and labor costs ultimately lead to a high product price. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] Japanese Patent Application Laid-Open No. 2008-264066 Summary of the Invention [Problem to be solved by the invention]

[0006] The present disclosure has been conceived under these circumstances, and a main object of the present disclosure is to provide a liquid collection container that allows collected liquid to be taken out in a closed state. [Means for solving the problem]

[0007] In order to solve the above problems, the present disclosure employs the following technical means.

[0008] A liquid collection container provided by a first aspect of the present disclosure includes a container body having a first cylindrical portion extending in a first direction, a gasket that forms a first space inside the container body by being in close contact with an inner peripheral surface of the first cylindrical portion in a circumferential direction without any gaps, a first switching portion that is disposed at one end of the first cylindrical portion in the first direction and is switchable between a state in which the first space is in communication with the outside and a state in which the first space is not in communication with the outside, and a first switching portion that is connected to the gasket and extends from an opening at the other end of the first cylindrical portion in the first direction to the other side in the first direction, and a plunger capable of applying force to slide the gasket in the first direction, the first switching portion including a blocking portion and one or more sets of first plug portions and a flow path tube, the blocking portion blocking one side end of the first cylindrical portion in the first direction and having one or more communicating holes penetrating in the first direction, the first plug portion including a first connecting portion that is gas-impermeable and liquid-tight, and the flow path tube having a first end connected to the blocking portion in a state communicating with the communicating hole and a second end closed by the first connecting portion.

[0009] A liquid component extraction system provided by a second aspect of the present disclosure is a liquid component extraction system in which each element is connected in a closed manner, and includes a liquid collection container according to the first aspect of the present disclosure, a power function unit that applies a pushing / pulling force to the plunger of the liquid collection container to move the gasket in the first direction, a second stopper portion connectable to the first stopper portion of the liquid collection container, a first flow path having one end connected to the second stopper portion, and a liquid storage container that connects to the other end of the first flow path and is used to store components in the first space of the container body.

[0010] Other features and advantages of the present disclosure will become more apparent from the following detailed description taken in conjunction with the accompanying drawings. [Brief explanation of the drawings]

[0011] [Figure 1] 1 is a front view showing a liquid collection container according to a first embodiment of the present disclosure. [Figure 2] FIG. 2 is a vertical cross-sectional view of the liquid collection container shown in FIG. [Figure 3] This shows the liquid collection container shown in FIG. 2 in a state where a specific gravity liquid is contained therein. [Figure 4] 1 is a longitudinal cross-sectional view showing a state in which a liquid collection container according to a first embodiment of the present disclosure is in use. [Figure 5] 1 is a longitudinal cross-sectional view showing a state in which a liquid collection container according to a first embodiment of the present disclosure is in use. [Figure 6] 1 is a longitudinal cross-sectional view showing a state in which a liquid collection container according to a first embodiment of the present disclosure is in use. [Figure 7] 1 is a schematic configuration diagram showing a liquid component extraction system according to a first embodiment of the present disclosure. [Figure 8] FIG. 2 is a diagram for explaining a method of using the liquid component extraction system according to the first embodiment of the present disclosure. [Figure 9] 10 is a longitudinal cross-sectional view similar to FIG. 2, showing a liquid collection container according to a second embodiment of the present disclosure. [Figure 10] 10 is a longitudinal cross-sectional view similar to FIG. 2, showing a liquid collection container according to a third embodiment of the present disclosure. [Figure 11] 10 is a longitudinal cross-sectional view similar to FIG. 2, showing a liquid collection container according to a fourth embodiment of the present disclosure. [Figure 12] FIG. 10 is a vertical cross-sectional view showing a state in which a liquid collection container according to a fourth embodiment of the present disclosure is in use. [Figure 13] FIG. 10 is a schematic configuration diagram showing a liquid component extraction system according to a second embodiment of the present disclosure. [Figure 14] FIG. 10 is a schematic configuration diagram showing a liquid component extraction system according to a third embodiment of the present disclosure. DETAILED DESCRIPTION OF THE INVENTION

[0012] Preferred embodiments of the present disclosure will now be described in detail with reference to the drawings.

[0013] Terms such as "first," "second," and "third" in this disclosure are used merely as labels and are not necessarily intended to dictate any ordering of their objects.

[0014] 1 to 3 show a liquid collection container according to a first embodiment of the present disclosure. The liquid collection container A1 of this embodiment comprises a container body 1, a gasket 2, a plurality of first stoppers 3, a plurality of flow path tubes 4, and a plunger 5. The liquid collection container A1 of this embodiment is used, for example, to collect blood and separate and recover white blood cells, including mononuclear cells, from other components in the blood by centrifugation. FIG. 1 is a front view of the liquid collection container A1. FIG. 2 is a longitudinal cross-sectional view of the liquid collection container A1.

[0015] In this embodiment, the container body 1 has a first cylindrical portion 11 and a closing wall 12. The first cylindrical portion 11 is roughly cylindrical and extends in the first direction x. The closing wall 12 closes one end of the first cylindrical portion 11 in the first direction x (hereinafter referred to as the "first direction one side x1 end" where appropriate). The closing wall 12 has a communication hole 121. The communication hole 121 is a hole that penetrates in the first direction x. In the illustrated example, the closing wall 12 has two communication holes 121. The first cylindrical portion 11 has an opening 111 at the other end in the first direction x (hereinafter referred to as the "first direction other side x2 end" where appropriate).

[0016] The gasket 2 is fitted into the first cylindrical portion 11 and is tightly fitted to the inner peripheral surface of the first cylindrical portion 11 in the circumferential direction. The gasket 2 is made of, for example, a rubber material with appropriate elasticity. The shape of the gasket 2 is not particularly limited, and in the example shown, the gasket 2 is configured such that two ring-shaped protrusions are connected to each other in a line in the first direction x on the outer periphery. The material of the gasket 2 is not particularly limited, and examples thereof include TPE (thermoplastic elastomer). The gasket 2 forms a closed first space S1 inside the first cylindrical portion 11 (container body 1). The first space S1 is located on one side x1 in the first direction with respect to the gasket 2. The gasket 2 is able to slide in the first direction x while being tightly fitted to the inner peripheral surface of the first cylindrical portion 11.

[0017] The plurality of first plug portions 3 and the plurality of flow path tubes 4 are arranged at the end of the first cylindrical portion 11 on one side x1 in the first direction.

[0018] Regarding the first stopper portion 3 and the flow path tube 4, one first stopper portion 3 and one flow path tube 4 constitute one set, and the liquid collection container A1 comprises one or more sets of first stopper portions 3 and flow path tubes 4. In this embodiment, the liquid collection container A1 comprises two sets of first stopper portions 3 and flow path tubes 4 (two first stopper portions 3 and two flow path tubes 4). In the illustrated example, the two first stopper portions 3 have mutually different structures. In the following description, in Figures 1 to 3, etc., the first stopper portion 3 on the left side of the figures will be referred to as the "first stopper portion 3A" as appropriate, and the first stopper portion 3 on the right side of the figures will be referred to as the "first stopper portion 3B" as appropriate, to distinguish between them.

[0019] In the illustrated example, the first plug portion 3A is configured as a needleless connector. The first plug portion 3A is one connector portion of the needleless connector and has a first connection portion 31. The first connection portion 31 has a valve structure that can be switched between an open and closed state. The first connection portion 31 is closed when the first plug portion 3A is not connected to the connector (the state shown in Figure 2), and opens when the first plug portion 3A is connected to the connector. Note that the shape, etc. of the first plug portion 3A is not limited to the illustrated example.

[0020] In the illustrated example, the first plug portion 3B is configured as a needleless connector. The first plug portion 3B is a connector portion on one side of the needleless connector and has a first connection portion 32. The first connection portion 32 has a valve structure that can be switched between an open and closed state. The first connection portion 32 is closed when the first plug portion 3B is not connected to the connector (the state shown in FIG. 2), and opens when the first plug portion 3B is connected to the connector. In the illustrated example, the first plug portion 3B is a connector portion that can be connected to the first plug portion 3A. Note that the shape, etc. of the first plug portion 3B is not limited to the illustrated example.

[0021] The flow path tube 4 is a fluid flow path that connects the blocking wall 12 and the first plug portion 3 (first plug portion 3A or first plug portion 3B), and is made of, for example, a flexible material. The flow path tube 4 has a first end portion 41 and a second end portion 42 at both ends. The first end portion 41 is fitted into, for example, a communication hole 121 in the blocking wall 12, and the first end portion 41 and the blocking wall 12 are connected in a sealed state by an appropriate means such as adhesive. The first end portion 41 is connected to the blocking wall 12 in a state in which it communicates with the communication hole 121, and the inside of the flow path tube 4 communicates with the first space S1 of the first cylindrical portion 11 (container body 1).

[0022] The second end 42 is connected in a sealed state to the end of the first plug portion 3 (first plug portions 3A, 3B) by an appropriate means such as adhesive. As shown in Fig. 2, when the first plug portion 3A (3B) is not connected to the connector, the inside of the flow path tube 4 is isolated from the outside by the first connection portion 31 (32) and does not communicate with the outside.

[0023] In the state shown in Fig. 2, the first space S1 of the container body 1 is not in communication with the outside due to the blocking wall 12, the two sets of first stoppers 3, and the flow path tube 4 configured as described above. On the other hand, by connecting, for example, a connector portion (for example, the connector portion 62 of the connector 6 described below with reference to Fig. 5), the first space S1 of the container body 1 can be in communication with the outside via the flow path tube 4. The blocking wall 12, the two sets of first stoppers 3, and the flow path tube 4 are one example of components of the "first switching portion." Furthermore, the two sets of first stoppers 3 and the flow path tube 4 can be handled as a single assembly part.

[0024] The plunger 5 applies force to slide the gasket 2 in the first direction x. The plunger 5 is connected to the gasket 2 and extends from an opening 111 at the end of the container body 1 (first cylindrical portion 11) on the other side x2 in the first direction toward the other side x2 in the first direction. In the illustrated example, the plunger 5 has a shaft portion 51, a tip portion 52, and a rear end portion 53. The shaft portion 51 is elongated in the first direction x. The tip portion 52 is located at the end of the shaft portion 51 on the one side x1 in the first direction, and the gasket 2 is attached to the shaft portion 51. The rear end portion 53 is located at the end of the shaft portion 51 on the other side x2 in the first direction, and is flange-shaped and extends radially outward beyond the shaft portion 51.

[0025] In the liquid collection container A1 of this embodiment, as shown in FIG. 3, a specific gravity liquid 80 is contained in the first space S1 of the container body 1. The specific gravity liquid 80 is arranged on one side x1 in the first direction of the gasket 2. The specific gravity liquid 80 is used to separate specific components in blood. In this embodiment, the specific gravity liquid 80 is suitable for separating plasma, white blood cells including mononuclear cells, and red blood cells in blood. If necessary, a blood anticoagulant may be additionally contained in the first space S1 of the container body 1.

[0026] Next, the method of use and operation of the liquid collection container A1 will be described with reference to FIGS.

[0027] The liquid collection container A1 is used to collect blood in the container body 1, separate white blood cells including mononuclear cells in the blood from other components by centrifugation, and recover them.

[0028] As shown in FIGS. 4 and 5, when blood is collected into the container body 1 of the liquid-collection container A1, a connector 62 is connected to the first stopper 3A (first connecting portion 31), for example. The connector 62 is provided at an end of a connector 6 having a tube 61 and is connectable to the first stopper 3A. A blood bag containing blood collected from a human body is connected to the other end of the tube 61. When the connector 62 is connected to the first stopper 3A, the first connecting portion 31 is opened. Next, when the plunger 5 is pulled toward the other side x2 in the first direction, blood flows from the blood bag (outside) into the first space S1, allowing blood to be collected. Note that the connector 6 is not limited to the configuration in which a blood bag is connected to the other end as described above. For example, the connector 6 may be a blood collection tool having a blood collection needle attached to the other end. When the connector 6 is a blood collection tool, the blood collection needle is inserted into a vein in the body and the connector 62 is connected to the first stopper 3A. Next, when the plunger 5 is pulled out to the other side x2 in the first direction, blood flows directly from the body (outside) into the first space S1, and the blood can be collected.

[0029] 5 shows blood 82 collected inside the container body 1 (first space S1). After blood 82 has been collected in the container body 1, the connector part 62 of the connector 6 is removed from the first stopper part 3A (first connection part 31). This closes the first connection part 31, and the first space S1 is no longer in communication with the outside.

[0030] Next, the flow path tube 4 connected to the first stopper 3A is heat-sealed to form a closed seal 43, and the first stopper 3A is removed (see FIG. 6). The liquid collection container A1 is then placed in, for example, a centrifuge and centrifuged. Centrifuging the liquid collection container A1 separates the blood components, and as shown in FIG. 6, red blood cells 83, specific gravity solution 80, white blood cells 84 including mononuclear cells, and plasma 85 are layered in this order in the first space S1 of the liquid collection container A1 from the other side x2 in the first direction relative to the gasket 2. After the centrifugation process, the liquid collection container A1 is held with the container body 1 in a substantially vertical position to maintain the layered state of the blood components shown in FIG. 6.

[0031] 7 and 8, a liquid component removal system C1 is used to recover some of the blood components in the container 1. In this embodiment, a case will be described in which white blood cells 84, including mononuclear cells, are recovered from the container 1. The mononuclear cells are the raw material for iPS cells used in regenerative medicine and the like.

[0032] 7 shows a liquid component extraction system C1 according to the first embodiment of the present disclosure. The liquid component extraction system C1 includes the liquid collection container A1, a liquid component extraction unit B1, and a power function part 990, and extracts liquid components from the container body 1 (liquid collection container A1).

[0033] The liquid component removal unit B1 (liquid component removal system C1) includes a second stopper 901, a first flow path 902, a joint 903, a second flow path 904, a first liquid storage container 905, a first valve 906, a third flow path 907, a second liquid storage container 908, a second valve 909, a fourth flow path 911, a sterile air supply member 912, and a pump function unit 913, and each element is configured to be connected in a closed manner. Each of the first flow path 902, second flow path 904, third flow path 907, and fourth flow path 911 is, for example, a tube made of a flexible material.

[0034] The second stopper 901 is connectable to the first stopper 3B of the liquid-collecting container A1. One end of the first flow path 902 is connected to the second stopper 901. When the second stopper 901 is connected to the first stopper 3B, the inside of the container body 1 and the first flow path 902 are connected to each other. The other end of the first flow path 902 is connected to a joint 903. In this embodiment, the joint 903 is, for example, a four-way joint, and in addition to the first flow path 902, one end of each of the second flow path 904, the third flow path 907, and the fourth flow path 911 is connected to the joint 903. The joint 903 interconnects the first flow path 902, the second flow path 904, the third flow path 907, and the fourth flow path 911.

[0035] One end of the second flow path 904 is connected to a joint 903, and communicates with the first flow path 902 via the joint 903. The other end of the second flow path 904 is connected to a first liquid storage container 905. In this embodiment, the first liquid storage container 905 is for storing plasma 85 (first component) in the first space S1 of the container body 1. The first liquid storage container 905 is preferably flexible and configured so that its volume can be changed. An example of such a first liquid storage container 905 is a film-like bag. A first valve 906 is provided in the second flow path 904. The first valve 906 is an open / close valve that can switch the second flow path 904 between an open state and a closed state.

[0036] One end of the third flow path 907 is connected to a joint 903, and communicates with the first flow path 902 via the joint 903. The other end of the third flow path 907 is connected to a second liquid storage container 908. In this embodiment, the second liquid storage container 908 is used to store white blood cells 84 (second component) including mononuclear cells in the first space S1 of the container body 1. The second liquid storage container 908 previously stores, for example, a vector incorporating factors necessary for establishing iPS cells. The second liquid storage container 908 is preferably flexible and configured to have a variable volume. An example of such a second liquid storage container 908 is a film-like bag. A second valve 909 is provided in the third flow path 907. The second valve 909 is an open / close valve that can switch the third flow path 907 between an open state and a closed state. Although not shown, a culture vessel can be connected to the second liquid storage container 908 in a closed state, and the fluid in the second liquid storage container 908 can be pumped into the culture vessel.

[0037] The types of the first valve 906 and the second valve 909 are not particularly limited. Each of the first valve 906 and the second valve 909 is, for example, a pinch valve. The pinch valves sandwich the respective flow paths 904, 907, which are made of tubes, and are in a closed state. Each of the first valve 906 and the second valve 909 (pinch valves) may be either an electric or manual type.

[0038] One end of fourth flow path 911 is connected to joint 903 and communicates with both second flow path 904 and third flow path 907 via joint 903. The other end of fourth flow path 911 is connected to sterile air supply member 912. In this embodiment, sterile air is pre-filled in sterile air supply member 912. Sterile air supply member 912 is configured, for example, to have flexibility and a variable volume. Examples of such sterile air supply member 912 include a three-dimensionally molded bag and a bellows-like bag. Furthermore, sterile air supply member 912 may be configured, for example, with a vent filter instead of a container such as the three-dimensionally molded bag or bellows-like bag. In this case, it is possible to sterilize air flowing from the outside into fourth flow path 911 via the vent filter. Pump function unit 913 is provided in fourth flow path 911. Pump function unit 913 is for sending sterilized air from sterilized air supply member 912 via fourth flow path 911 and joint unit 903 to either second flow path 904 or third flow path 907. Pump function unit 913 is, for example, a suction pump.

[0039] The power function unit 990 applies a pushing / pulling force to the plunger 5 of the liquid collection container A1, thereby moving the gasket 2 toward the one side x1 in the first direction. The power function unit 990 includes a movable part 991. The movable part 991 engages with the rear end part 53 of the plunger 5. The power function unit 990 is configured, for example, by an actuator, and by driving the actuator, the movable part 991 is moved toward the one side x1 in the first direction. As a result, the plunger 5 and the gasket 2 connected thereto are pushed toward the one side x1 in the first direction.

[0040] Next, with reference to FIG. 8, a method for recovering a liquid component in the liquid collection container A1 (container body 1) using the liquid component recovery system C1 will be described.

[0041] First, the second plug 901 of the liquid component extraction unit B1 (liquid component extraction system C1) is connected to the first plug 3B of the liquid collection container A1. As a result, the first space S1 on the one side x1 in the first direction of the gasket 2 of the liquid collection container A1 communicates with the first flow path 902. The first space S1 is kept closed by the gasket 2. The first valve 906 and the second valve 909 of the liquid component extraction unit B1 are both closed in advance.

[0042] Next, the first valve 906 is opened, and the power function unit 990 is activated. By this activation of the power function unit 990, for example, the movable unit 991 is moved at a constant, slow speed toward the first direction side x1, and the gasket 2 is moved toward the first direction side x1. Then, the plasma 85 at the end of the first direction side x1 in the first space S1 flows into the second flow path 904 via the flow path tube 4, the first flow path 902, and the joint unit 903, and is sent toward the first liquid storage container 905. The flow of this plasma 85 is indicated by arrow N1 in FIG. 8 . In this way, the unnecessary component, plasma 85 (first component), is stored in the first liquid storage container 905. When almost all of the plasma 85 has flowed into the second flow path 904, the first valve 906 is closed.

[0043] Next, the second valve 909 is opened. Operation of the power function unit 990 causes the gasket 2 in the container body 1 to move further toward the first direction side x1. Then, the white blood cells 84 containing mononuclear cells in the first space S1 flow through the flow path tube 4, the first flow path 902, and the joint unit 903 into the third flow path 907 and are sent toward the second liquid storage container 908. This flow of the white blood cells 84 containing mononuclear cells is indicated by arrow N2 in FIG. 8. In this way, the white blood cells 84 containing mononuclear cells (second component), which are a necessary component, are stored in the second liquid storage container 908. When almost all of the white blood cells 84 containing mononuclear cells have flowed into the third flow path 907, the power function unit 990 is stopped.

[0044] Next, pump function unit 913 is operated. As a result, the sterile air in sterile air supply member 912 flows through fourth flow path 911 and joint unit 903 into third flow path 907, and is sent toward second liquid storage container 908. This flow of sterile air is indicated by arrow N3 in Figure 8. White blood cells 84, including mononuclear cells, remaining in joint unit 903 and third flow path 907 are pushed by the sterile air and sent toward second liquid storage container 908. When the sterile air has flowed into almost the entire third flow path 907, pump function unit 913 is stopped, and second valve 909 is closed.

[0045] In the second liquid storage container 908, the vector stored therein is mixed with the white blood cells 84 containing monocytes sent from the liquid collection container A1. Next, after a predetermined time has elapsed, a culture vessel (not shown) is connected to the second liquid storage container 908, the second valve 909 is opened, and the pump function unit 913 is operated. As a result, the fluid in the second liquid storage container 908 (a mixture of the vector and the white blood cells 84 containing monocytes) is pushed out by the sterile air sent from the sterile air supply member 912 through the third flow path 907 and sent to the culture vessel located downstream. Once the fluid in the second liquid storage container 908 has been sent to the culture vessel, the pump function unit 913 is stopped and the second valve 909 is closed. This completes the process of recovering liquid components from the liquid collection container A1 (container body 1) using the liquid component removal system C1.

[0046] In the liquid collection container A1, as shown in FIGS. 2 and 3, a gasket 2 is fitted into the first cylindrical portion 11 of the container body 1. The gasket 2 is tightly fitted to the inner circumferential surface of the first cylindrical portion 11 in the circumferential direction, thereby defining a first space S1 on one side x1 in the first direction inside the container body 1. A blocking wall 12, one or more sets of first stoppers 3, and a flow path tube 4 (first switching portion) are arranged at the end of the first cylindrical portion 11 on the one side x1 in the first direction. The blocking wall 12, the first stopper 3, and the flow path tube 4 are switchable between a state in which the first space S1 is connected to the outside and a state in which the first space S1 is not connected to the outside. With this configuration, when the connector portion 62 is connected to the first stopper 3A (first connection portion 31), blood (liquid) can flow from the blood bag (outside) into the first space S1 while maintaining the closed state.

[0047] After separating the blood components in the first space S1, as described with reference to Figures 7, 8, etc., the liquid components in the liquid collection container A1 (container body 1) can be extracted by using a liquid component extraction system C1 equipped with a liquid collection container A1, a liquid component extraction unit B1, and a power function part 990.

[0048] The liquid component extraction unit B1 (liquid component extraction system C1) includes a second stopper 901, a first flow path 902, a joint 903, a second flow path 904, a first liquid storage container 905, a first valve 906, a third flow path 907, a second liquid storage container 908, a second valve 909, a fourth flow path 911, a sterile air supply member 912, and a pump function unit 913, with each element being connected in a closed manner. The second stopper 901 is connectable to the first stopper 3B of the liquid collection container A1. One end of the first flow path 902 is connected to the second stopper 901. The other end of the first flow path 902 is connected to the joint 903. One end of the second flow path 904 is connected to the joint 903 and communicates with the first flow path 902 via the joint 903. The other end of the second flow path 904 is connected to the first liquid storage container 905. The first liquid storage container 905 is for storing plasma 85 (first component), an unnecessary component, in the first space S1 of the container body 1. The first valve 906 is provided in the second flow path 904. One end of the third flow path 907 is connected to a joint 903 and communicates with the first flow path 902 via the joint 903. The other end of the third flow path 907 is connected to a second liquid storage container 908. The second liquid storage container 908 is for storing white blood cells 84 (second component), including mononuclear cells, a necessary component, in the first space S1 of the container body 1. The second valve 909 is provided in the third flow path 907. The liquid component removal system C1 also includes a power function unit 990. The power function unit 990 applies a pushing / pulling force to the plunger 5 of the liquid collection container A1, thereby moving the gasket 2 toward one side x1 in the first direction. With this configuration, by appropriately operating the power function unit 990 as described above and appropriately switching the opening and closing of the first valve 906 and the second valve 909, it is possible to separate the white blood cells 84 (second component) containing the necessary component monocytes in the first space S1 from the unnecessary component plasma 85 (first component) in the first space S1, and remove them in a closed state without opening the container body 1.

[0049] The liquid component removal unit B1 (liquid component removal system C1) includes a fourth flow path 911, a sterile air supply member 912, and a pump function unit 913. One end of the fourth flow path 911 is connected to a joint 903 and communicates with both the second flow path 904 and the third flow path 907 via the joint 903. The other end of the fourth flow path 911 is connected to a sterile air supply member 912. The sterile air supply member 912 contains sterile air. The pump function unit 913 is provided in the fourth flow path 911. The pump function unit 913 sends sterile air from the sterile air supply member 912 to either the second flow path 904 or the third flow path 907 via the fourth flow path 911 and the joint 903. With this configuration, white blood cells 84, including mononuclear cells, remaining in the joint 903 and the third flow path 907 can be sent to the second liquid storage container 908 by being swept away by the sterile air. This makes it possible to efficiently collect the necessary component even when the amount of white blood cells 84 (second component) containing monocytes, which is a necessary component to be collected, is small. Furthermore, with the above configuration, it is possible to send plasma 85 remaining in joint portion 903 and second flow path 904 toward first liquid storage container 905. This makes it possible to prevent plasma 85 (first component), which is an unnecessary component, from mixing with white blood cells 84 (second component) containing monocytes, which is a necessary component, and to efficiently collect the monocytes.

[0050] Figure 9 shows a liquid collection container according to a second embodiment of the present disclosure. Compared to the liquid collection container A1 of the above embodiment, the liquid collection container A2 of this embodiment further includes an outer envelope 7, which is a major difference from the liquid collection container A1 described above. In Figure 9 and subsequent figures, elements that are the same as or similar to those of the liquid collection container A1 of the above embodiment are designated by the same reference numerals as in the above embodiment, and descriptions thereof will be omitted where appropriate.

[0051] The outer case 7 at least blocks the opening 111 at the end of the first cylindrical portion 11 on the other side x2 in the first direction from the outside. The outer case 7 is flexible, allowing the plunger 5 to slide in the first direction x by applying a pushing or pulling force from outside the outer case 7. In this embodiment, the outer case 7 has a front end 71 and a rear end 72, which are connected in a bellows-like manner. The front end 71 is located on the one side x1 in the first direction of the outer case 7, and the rear end 72 is located on the other side x2 in the first direction of the outer case 7. In this embodiment, a flange 13 is formed on the end of the first cylindrical portion 11 on the one side x1 in the first direction. The front end 71 of the outer case 7 is hermetically joined to the flange 13. Furthermore, the rear end 53 of the plunger 5 is hermetically joined to the rear end 72 (the end of the outer case 7 on the other side x2 in the first direction). This prevents the interior space of the outer case 7 from communicating with the outside. Therefore, the opening 111 at the end on the other side x2 in the first direction of the first cylindrical portion 11 is sealed off from the outside, and the sterility of the container body 1 is appropriately maintained.

[0052] In the liquid collection container A2, a gasket 2 is fitted into the first cylindrical portion 11 of the container body 1. The gasket 2 fits tightly against the inner circumferential surface of the first cylindrical portion 11 in the circumferential direction, thereby defining a first space S1 on one side x1 in the first direction inside the container body 1. A blocking wall 12, one or more sets of first stoppers 3, and a flow path tube 4 (first switching portion) are arranged at the end of the first side x1 in the first direction of the first cylindrical portion 11. The blocking wall 12, the first stopper 3, and the flow path tube 4 are switchable between a state in which the first space S1 is connected to the outside and a state in which the first space S1 is not connected to the outside. With this configuration, when the connector portion 62 is connected to the first stopper 3A (first connection portion 31), blood (liquid) can flow from the blood bag (outside) into the first space S1 while maintaining the closed state.

[0053] After separating the blood components in the first space S1, the liquid components can be extracted from the liquid collection container A2 (container body 1) by using a liquid component extraction system including the liquid collection container A2, the liquid component extraction unit B1 described with reference to Figures 7 and 8 for the liquid component extraction system C1 according to the first embodiment, and the power function part 990. Here, the same effects as those described with reference to Figures 7 and 8 for the liquid component extraction system C1 according to the first embodiment can be achieved.

[0054] The liquid collection container A2 of this embodiment further ensures sterility of the container. This allows the cleanliness level of the usage environment to be lowered, both in the applications of the present disclosure and in other applications. For example, by eliminating the need for equipment such as an isolator, it is possible to reduce the cost of cell production. In applications other than those disclosed herein, the container A2 can also be suitably used for sample collection from cell culture containers and for adding or replacing culture medium components.

[0055] 10 shows a liquid collection container according to a third embodiment of the present disclosure. The liquid collection container A3 of this embodiment differs significantly from the liquid collection container A1 of the above embodiment in that it further includes an outer envelope 7.

[0056] The outer cover 7 at least blocks the opening 111 at the end of the first cylindrical portion 11 on the other side x2 in the first direction from the outside. The outer cover 7 is flexible, allowing the plunger 5 to slide in the first direction x by applying a pushing or pulling force from outside the outer cover 7. In this embodiment, the outer cover 7 is a film-like bag. The outer cover 7 has a front end 71 and a rear end 72. A pair of flow path tubes 4 are inserted into the front end 71, and the front end 71 is tightly joined to the flow path tubes 4. The rear end 72 is located near the rear end 53 of the plunger 5 and is closed. This prevents the interior space of the outer cover 7 from communicating with the outside. Therefore, the opening 111 at the end of the first cylindrical portion 11 on the other side x2 in the first direction is blocked from the outside. This allows the container 1 to be properly maintained in a sterile state.

[0057] In the liquid collection container A3, a gasket 2 is fitted into the first cylindrical portion 11 of the container body 1. The gasket 2 fits tightly against the inner circumferential surface of the first cylindrical portion 11 in the circumferential direction, thereby defining a first space S1 on one side x1 in the first direction inside the container body 1. A blocking wall 12, one or more sets of first stoppers 3, and a flow path tube 4 (first switching portion) are arranged at the end of the first cylindrical portion 11 on the one side x1 in the first direction. The blocking wall 12, the first stopper 3, and the flow path tube 4 are switchable between a state in which the first space S1 is connected to the outside and a state in which the first space S1 is not connected to the outside. With this configuration, when the connector portion 62 is connected to the first stopper 3A (first connection portion 31), blood (liquid) can flow from the blood bag (outside) into the first space S1 while maintaining the closed state.

[0058] After separating the blood components in the first space S1, the liquid components can be extracted from the liquid collection container A3 (container body 1) by using a liquid component extraction system including the liquid collection container A3, the liquid component extraction unit B1 described with reference to Figures 7 and 8 for the liquid component extraction system C1 according to the first embodiment, and the power function part 990. Here, the same effects as those described for the liquid component extraction system C1 according to the first embodiment with reference to Figures 7 and 8 are achieved.

[0059] The liquid collection container A3 of this embodiment further ensures sterility of the container. This allows the cleanliness level of the usage environment to be lowered, both in the applications of the present disclosure and in other applications. For example, by eliminating the need for equipment such as an isolator, it is possible to reduce the cost of cell production. In applications other than those disclosed herein, the container A3 can also be used favorably for sample collection from cell culture containers and for adding or replacing culture medium components.

[0060] 11 shows a liquid collection container according to a fourth embodiment of the present disclosure. The liquid collection container A4 of this embodiment differs from the liquid collection container A1 of the above embodiment in the configuration of the container body 1.

[0061] In this embodiment, the container body 1 further has a second cylindrical portion 14 and a flange portion 15. The second cylindrical portion 14 is located on one side x1 in the first direction of the first cylindrical portion 11, and has a radial dimension larger than that of the first cylindrical portion 11. The flange portion 15 extends radially outward from the end of the second cylindrical portion 14 on the one side x1 in the first direction.

[0062] In the liquid collection container A4, a gasket 2 is fitted into the first cylindrical portion 11 of the container body 1. The gasket 2 fits tightly against the inner circumferential surface of the first cylindrical portion 11 in the circumferential direction, thereby defining a first space S1 on one side x1 in the first direction inside the container body 1. A blocking wall 12, one or more sets of first stoppers 3, and a flow path tube 4 (first switching portion) are arranged at the end of the first side x1 in the first direction of the first cylindrical portion 11. The blocking wall 12, the first stopper 3, and the flow path tube 4 are switchable between a state in which the first space S1 is connected to the outside and a state in which the first space S1 is not connected to the outside. With this configuration, when the connector portion 62 is connected to the first stopper 3A (first connection portion 31), blood (liquid) can flow into the first space S1 from the blood bag (outside) while maintaining the closed state.

[0063] In the liquid collection container A4, the container body 1 has the second cylindrical portion 14 and flange portion 15. With this configuration, as shown in FIG. 12, for example, the second cylindrical portion 14 of the container body 1 can be fitted into the upper opening of a centrifuge tube 79 and locked by the flange portion 15. In this way, the liquid collection container A4 is supported by the centrifuge tube 79, and by setting the centrifuge tube 79 in a centrifuge, centrifugal separation can be performed easily and appropriately. It is preferable to take measures to fix the first stopper portion 3B and the flow path tube 4 connected to the first stopper portion 3B to the rotor so that they are not damaged during the centrifugation process.

[0064] After separating the blood components in the first space S1, the liquid components can be extracted from the liquid collection container A4 (container body 1) by using a liquid component extraction system including the liquid collection container A4, the liquid component extraction unit B1 described with reference to Figures 7 and 8 for the liquid component extraction system C1 according to the first embodiment, and the power function part 990. Here, the same effects as those described with reference to Figures 7 and 8 for the liquid component extraction system C1 according to the first embodiment can be achieved.

[0065] Fig. 13 shows a liquid component extraction system C2 according to a second embodiment of the present disclosure. The liquid component extraction system C2 comprises the above-described liquid collection container A1, a liquid component extraction unit B2, and a power function section 990, and extracts liquid components from the container body 1 (liquid collection container A1). In Fig. 13, elements that are the same as or similar to the liquid component extraction unit B1 of the above embodiment are designated by the same reference numerals as in the above embodiment, and descriptions thereof will be omitted where appropriate.

[0066] The liquid component extraction unit B2 (liquid component extraction system C2) comprises a third stopper portion 901, a first flow path 902, a joint portion 903, a second flow path 904, a first liquid storage container 905, a first valve 906, a third flow path 907, a second liquid storage container 908 and a second valve 909, and each element is connected in a closed manner.

[0067] In this embodiment, the joint portion 903 is, for example, a three-way joint, and is connected to one end of each of the second flow path 904 and the third flow path 907 in addition to the first flow path 902. The joint portion 903 interconnects the first flow path 902, the second flow path 904, and the third flow path 907.

[0068] Next, a method for recovering liquid components in a liquid collection container A1 (container body 1) using a liquid component recovery system C2 will be described with reference to Figure 13. In this embodiment, recovery of white blood cells 84, including mononuclear cells, in the container body 1 will be described. The white blood cells 84, including mononuclear cells, are used as a raw material for iPS cells used in regenerative medicine and the like.

[0069] First, the second plug 901 of the liquid component extraction unit B2 (liquid component extraction system C2) is connected to the first plug 3B of the liquid collection container A1. As a result, the first space S1 on the one side x1 in the first direction of the gasket 2 of the liquid collection container A1 communicates with the first flow path 902. The first space S1 is kept closed by the gasket 2. The first valve 906 and the second valve 909 are both closed in advance.

[0070] Next, the first valve 906 is opened, and the power function unit 990 is activated. By this activation of the power function unit 990, for example, the movable unit 991 is moved at a constant, slow speed toward the first direction side x1, and the gasket 2 is moved toward the first direction side x1. Then, the plasma 85 at the end of the first direction side x1 in the first space S1 flows into the second flow path 904 via the flow path tube 4, the first flow path 902, and the joint unit 903, and is sent toward the first liquid storage container 905. The flow of this plasma 85 is indicated by arrow N11 in FIG. 13 . In this way, the unnecessary component, plasma 85 (first component), is stored in the first liquid storage container 905. When almost all of the plasma 85 has flowed into the second flow path 904, the first valve 906 is closed.

[0071] Next, the second valve 909 is opened. Operation of the power function unit 990 causes the gasket 2 in the container body 1 to move further toward the first direction x1. The white blood cells 84, including mononuclear cells, in the first space S1 then flow through the flow path tube 4, the first flow path 902, and the joint unit 903 into the third flow path 907 and are sent toward the second liquid storage container 908. This flow of the white blood cells 84, including mononuclear cells, is indicated by arrow N12 in FIG. 13. In this manner, the necessary white blood cells 84, including mononuclear cells (second component), are stored in the second liquid storage container 908. When almost all of the white blood cells 84, including mononuclear cells, have flowed into the second liquid storage container 908, the power function unit 990 is stopped and the second valve 909 is closed. This completes the process of recovering liquid components from the liquid collection container A1 (container body 1) using the liquid component recovery system C2.

[0072] The liquid component extraction unit B2 (liquid component extraction system C2) includes a second stopper 901, a first flow path 902, a joint 903, a second flow path 904, a first liquid storage container 905, a first valve 906, a third flow path 907, a second liquid storage container 908, and a second valve 909, with each element being connected in a closed manner. The second stopper 901 is connectable to the first stopper 3B of the liquid collection container A1. One end of the first flow path 902 is connected to the second stopper 901. The other end of the first flow path 902 is connected to the joint 903. One end of the second flow path 904 is connected to the joint 903 and communicates with the first flow path 902 via the joint 903. The other end of the second flow path 904 is connected to the first liquid storage container 905. The first liquid storage container 905 is for storing plasma 85 (first component), an unnecessary component, in the first space S1 of the container body 1. The first valve 906 is provided in the second flow path 904. One end of the third flow path 907 is connected to a joint 903 and communicates with the first flow path 902 via the joint 903. The other end of the third flow path 907 is connected to a second liquid storage container 908. The second liquid storage container 908 is for storing white blood cells 84 (second component), including mononuclear cells, a necessary component, in the first space S1 of the container body 1. The second valve 909 is provided in the third flow path 907. The liquid component removal system C2 also includes a power function unit 990. The power function unit 990 applies a pushing / pulling force to the plunger 5 of the liquid collection container A1, thereby moving the gasket 2 toward one side x1 in the first direction. With this configuration, by appropriately operating the power function unit 990 as described above and appropriately switching the opening and closing of the first valve 906 and the second valve 909, it is possible to separate the white blood cells 84 (second component) containing the necessary component monocytes in the first space S1 from the unnecessary component plasma 85 (first component) in the first space S1, and remove them in a closed state without opening the container body 1.

[0073] 14 shows a liquid component extraction system C3 according to a third embodiment of the present disclosure. The liquid component extraction system C3 includes the liquid collection container A1 described above and the elements described below, and extracts liquid components from the inside of the container body 1 (liquid collection container A1). In this embodiment, blood, for example, is contained in the first space S1 within the container body 1 (liquid collection container A1). In this embodiment, blood collected from a human body is contained in the first space S1 of the container body 1 as is, without separation of blood components by centrifugation.

[0074] The liquid component extraction system C3 includes a liquid collection container A1, a second stopper 901, a first flow path 902, and a liquid storage container 921, with each element being connected in a closed manner. The first flow path 902 is, for example, a tube made of a flexible material. The liquid component extraction system C3 also includes a power function unit 990.

[0075] The second stopper 901 is connected to the first stopper 3B of the liquid collection container A1. One end of the first flow path 902 is connected to the second stopper 901. This allows communication between the inside of the container body 1 and the first flow path 902. The other end of the first flow path 902 is connected to a liquid storage container 921.

[0076] In this embodiment, the liquid storage container 921 is used to store blood in the first space S1 of the container body 1. The liquid storage container 921 previously contains, for example, a vector incorporating factors necessary for establishing iPS cells. The liquid storage container 921 is preferably flexible and configured so that its volume can be changed. An example of such a liquid storage container 921 is a film-like bag.

[0077] Next, a method for recovering liquid components in a liquid collection container A1 (container body 1) using the liquid component recovery system C3 will be described. In this embodiment, the case of recovering blood in the container body 1 will be described. Specific components in blood (e.g., white blood cells including monocytes) are used as raw materials for iPS cells used in regenerative medicine, etc.

[0078] The second plug 901 is connected to the first plug 3B of the liquid collection container A1. As a result, the first space S1 on the one side x1 in the first direction of the gasket 2 of the liquid collection container A1 communicates with the first flow path 902.

[0079] Next, the power function unit 990 is operated. By operating this power function unit 990, for example, the movable part 991 is moved at a constant, slow speed toward one side x1 in the first direction, and the gasket 2 is moved toward one side x1 in the first direction. Then, the blood in the first space S1 flows into the first flow path 902 via the flow path tube 4 and is sent toward the liquid storage container 921. In this way, the blood in the container body 1 is stored in the liquid storage container 921. When an appropriate amount of blood has been stored in the liquid storage container 921, the power function unit 990 is stopped. This completes the process of collecting liquid components (blood) from the liquid collection container A1 (container body 1) using the liquid component extraction system C3.

[0080] The liquid component extraction system C3 includes a second stopper 901, a first flow path 902, and a liquid storage container 921, all of which are connected in a closed state. The second stopper 901 is connectable to the first stopper 3B of the liquid collection container A1. One end of the first flow path 902 is connected to the second stopper 901. The other end of the first flow path 902 is connected to the liquid storage container 921. The liquid storage container 921 is used to store the liquid component (blood) in the first space S1 of the container body 1. The liquid component extraction system C3 also includes a power function unit 990. The power function unit 990 applies a pushing / pulling force to the plunger 5 of the liquid collection container A1, moving the gasket 2 toward one side x1 in the first direction. With this configuration, by operating the power function unit 990, the liquid component (blood) in the first space S1 can be extracted in a closed state without opening the container body 1.

[0081] While specific embodiments of the present disclosure have been described above, the liquid collection container and liquid component extraction system according to the present disclosure are not limited to the above-described embodiments. The specific configurations of the components of the liquid collection container and liquid component extraction system according to the present disclosure can be freely designed in various ways.

[0082] The use of the liquid collection container of the present disclosure is not particularly limited, and it may be used for purposes other than separating and collecting mononuclear cells from blood, and is also suitable for separating and collecting, for example, plasma and PRP (platelet-rich plasma).

[0083] In the above embodiment, the first space S1 of the container body 1 was prefilled with specific gravity liquid 80, and blood was poured therein and centrifuged to obtain a layer of white blood cell components containing the target mononuclear cells, but the present disclosure is not limited to this. The liquid collection container of the present disclosure may be configured so that the first space of the container body is further prefilled with, for example, a float member or gel agent having a density between the density of red blood cells and the density of plasma, making it more difficult for the white blood cell component layer to mix with other layers.

[0084] Furthermore, the liquid collection container of the present disclosure does not necessarily require a centrifugation process. For example, when establishing iPS cells, blood may be collected and mixed with vectors or the like without separation. Even in this case, the advantage of being able to perform the work while maintaining a completely closed system can be fully enjoyed.

[0085] The liquid collected inside the container body may be, for example, fat cells, and is not limited to blood.

[0086] In the above embodiment, an example of a connection structure using a connector is shown for the first plug portion (3A, 3B), but the connection structure of the first plug portion is not particularly limited. In the present disclosure, the first plug portion may have a structure in which, for example, tubes or connector portions connected to tubes are joined in a sterile manner by welding or other means.

[0087] In the above embodiment, as an example of a closing portion of the present disclosure, the container body 1 has a closing wall 12, and the closing wall 12 closes the one side x1 in the first direction of the first cylindrical portion 11. However, the present disclosure is not limited to this. For example, the one side x1 in the first direction of the first cylindrical portion 11 may be an open end, and a plug member (e.g., made of rubber) serving as a closing portion may be press-fitted into the open end to close the one side x1 in the first direction of the first cylindrical portion 11. In this case, a communication hole may be formed in the plug member, and the first end 41 of the flow path tube 4 may be connected to the plug member in a state where it communicates with the first space S1 via the communication hole.

[0088] The present disclosure includes configurations relating to the following notes.

[0089] [Appendix 1] a container body having a first cylindrical portion extending in a first direction; a gasket that forms a first space inside the container body by being tightly attached to an inner peripheral surface of the first cylindrical portion in a circumferential direction without any gap; a first switching portion that is arranged at one end of the first cylindrical portion in the first direction and that is switchable between a state in which the first space is in communication with the outside and a state in which the first space is not in communication with the outside; a plunger connected to the gasket and extending from an opening at the other end of the first cylindrical portion in the first direction to the other side in the first direction, the plunger being capable of applying force to slide the gasket in the first direction; the first switching unit includes a blocking unit and one or more pairs of a first plug unit and a flow path tube; the blocking portion blocks one end of the first cylindrical portion in the first direction and has one or more communication holes penetrating in the first direction, the first plug portion includes a first connection portion that is gas impermeable and liquid-tight; The flow path tube has a first end connected to the closing portion in a state of communicating with the communication hole, and a second end closed by the first connecting portion. [Appendix 2] Further, an outer cover is provided to seal off the opening at the other end of the first cylindrical portion in the first direction from the outside. A liquid collection container as described in Appendix 1, wherein the outer casing is flexible and a pushing / pulling force can be applied to the plunger from outside the outer casing to slide the gasket in the first direction. [Appendix 3] A liquid collection container according to Appendix 2, wherein the other end of the plunger in the first direction is joined to the other end of the outer cover in the first direction. [Appendix 4] A liquid collection container as described in any one of appendixes 1 to 3, wherein the container body further has a second cylindrical portion located on one side of the first cylindrical portion in the first direction and having a radial dimension larger than that of the first cylindrical portion, and a flange portion extending radially outward from one end of the second cylindrical portion in the first direction. [Appendix 5] 5. The liquid collection container according to claim 4, wherein a specific gravity liquid is contained in the first space of the container body. [Appendix 6] A liquid component extraction system in which each element is configured to be closed and connected, A liquid collection container according to any one of appendices 1 to 5; a power function unit that applies a force to push or pull the plunger of the liquid collection container to move the gasket in the first direction; a second stopper portion connectable to the first stopper portion of the liquid collection container; a first flow path having one end connected to the second plug portion; a liquid storage container connected to the other end of the first flow path and configured to store the component in the first space of the container body; [Appendix 7] A liquid component extraction system in which each element is configured to be closed and connected, A liquid collection container according to any one of appendices 1 to 5; a power function unit that applies a force to push or pull the plunger of the liquid collection container to move the gasket in the first direction; a second stopper portion connectable to the first stopper portion of the liquid collection container; a first flow path having one end connected to the second plug portion; a joint portion to which the other end of the first flow path is connected; a second flow path having one end connected to the joint portion and communicating with the first flow path via the joint portion; a first liquid storage container connected to the other end of the second flow path and configured to store a first component in the first space of the container body; a third flow path having one end connected to the joint portion and communicating with the first flow path via the joint portion; a second liquid storage container connected to the other end of the third flow path and configured to store a second component in the first space of the container body; a first valve provided in the second flow path and capable of switching the second flow path between an open state and a closed state; a second valve provided in the third flow path and capable of switching the third flow path between an open state and a closed state; [Appendix 8] a fourth flow path having one end connected to the joint portion and communicating with the second flow path and the third flow path via the joint portion; a sterile air supply member connected to the other end of the fourth flow path; The liquid component extraction system described in Appendix 7, further comprising a pump function unit for sending sterile air from the sterile air supply member to either the second flow path or the third flow path via the fourth flow path and the joint unit. [Explanation of symbols]

[0090] A1, A2, A3, A4: liquid collection container, B1, B2: liquid component extraction unit, C1, C2, C3: liquid component extraction system, 1: container body, 11: first cylindrical portion, 111: opening, 12: blocking wall (blocking portion, first switching portion), 121: communication hole, 13: flange portion, 14: second cylindrical portion, 15: flange portion, 2: gasket, 3, 3A, 3B: first stopper portion (first switching portion), 31, 32: first connecting portion, 4: flow path tube (first switching portion), 41: first end portion, 42: second end portion, 43: seal portion, 5: plunger, 51: shaft portion, 52: tip portion, 53: rear end portion, 6: connector, 61: tube, 62: connector tap portion, 7: outer envelope, 71: front end portion, 72: rear end portion, 79: centrifuge tube, 80: specific gravity solution, 82: blood, 83: red blood cells, 84: white blood cells including mononuclear cells, 85: plasma, 901: second stopper portion, 902: first flow path, 903: joint portion, 904: second flow path, 905: first liquid storage container, 906: first valve, 907: third flow path, 908: second liquid storage container, 909: second valve, 911: fourth flow path, 912: sterilized air supply member, 921: liquid storage container, 990: power function portion, 991: moving portion, N1, N2, N3, N11, N12: arrows, S1: first space, x: first direction, x1: one side of the first direction, x2: other side of the first direction

Claims

1. a container body having a first cylindrical portion extending in a first direction; a gasket that forms a first space inside the container body by being tightly attached to an inner peripheral surface of the first cylindrical portion in a circumferential direction without any gap; a first switching portion that is arranged at one end of the first cylindrical portion in the first direction and that is switchable between a state in which the first space is in communication with the outside and a state in which the first space is not in communication with the outside; a plunger connected to the gasket and extending from an opening at the other end of the first cylindrical portion in the first direction to the other side in the first direction, the plunger being capable of applying a force to slide the gasket in the first direction; the first switching unit includes a blocking unit and one or more pairs of a first plug unit and a flow path tube; the blocking portion blocks one end of the first cylindrical portion in the first direction and has one or more communication holes penetrating in the first direction, the first plug portion includes a first connection portion that is gas impermeable and liquid-tight; The flow path tube has a first end connected to the closing portion in a state of communicating with the communication hole, and a second end closed by the first connecting portion.

2. an outer cover that seals off at least the opening at the other end of the first cylindrical portion in the first direction from the outside; 2. The liquid collection container according to claim 1, wherein the outer cover is flexible and a force for pushing or pulling the plunger can be applied from the outside of the outer cover to slide the gasket in the first direction.

3. 3. The liquid collection container according to claim 2, wherein the other end of the plunger in the first direction is joined to the other end of the outer cover in the first direction.

4. 4. A liquid collection container according to claim 1, wherein the container body further comprises: a second cylindrical portion located on one side of the first cylindrical portion in the first direction and having a radial dimension larger than that of the first cylindrical portion; and a flange portion extending radially outward from one end of the second cylindrical portion in the first direction.

5. The liquid collection container according to claim 4 , wherein the first space of the container body contains a specific gravity liquid.

6. A liquid component extraction system in which each element is configured to be closed and connected, A liquid collection container according to any one of claims 1 to 3; a power function unit that applies a force to push or pull the plunger of the liquid collection container to move the gasket in the first direction; a second stopper portion connectable to the first stopper portion of the liquid collection container; a first flow path having one end connected to the second plug portion; a liquid storage container connected to the other end of the first flow path and configured to store the component in the first space of the container body;

7. A liquid component extraction system in which each element is configured to be closed and connected, A liquid collection container according to any one of claims 1 to 3; a power function unit that applies a force to push or pull the plunger of the liquid collection container to move the gasket in the first direction; a second stopper portion connectable to the first stopper portion of the liquid collection container; a first flow path having one end connected to the second plug portion; a joint portion to which the other end of the first flow path is connected; a second flow path having one end connected to the joint portion and communicating with the first flow path via the joint portion; a first liquid storage container connected to the other end of the second flow path and configured to store a first component in the first space of the container body; a third flow path having one end connected to the joint portion and communicating with the first flow path via the joint portion; a second liquid container connected to the other end of the third flow path and configured to store the second component in the first space of the container body; a first valve provided in the second flow path and capable of switching the second flow path between an open state and a closed state; a second valve provided in the third flow path and capable of switching the third flow path between an open state and a closed state.

8. a fourth flow path having one end connected to the joint portion and communicating with the second flow path and the third flow path via the joint portion; a sterile air supply member connected to the other end of the fourth flow path; The liquid component extraction system according to claim 7, further comprising a pump function unit for sending sterile air from the sterile air supply member to either the second flow path or the third flow path via the fourth flow path and the joint unit.

Images