Cosmetic composition containing a eutectic mixture

Abstract

To provide a cosmetic composition which has excellent keratin exfoliation effect, causes less irritation, and has excellent low-temperature stability under weakly acidic or neutral conditions, and maintains excellent keratin exfoliation effect even under severe conditions, by incorporating purified water, AHA and an amino acid; two types of amino acids; or purified water and two types of amino acids in the form of a eutectic mixture in the cosmetic composition.SOLUTION: A cosmetic composition comprising a eutectic mixture containing: an amino acid, an alpha-hydroxy acid, and purified water; or two types of amino acids is prepared, wherein the cosmetic composition comprises either an amino acid and an alpha-hydroxy acid or two types of amino acids in the form of a eutectic mixture.SELECTED DRAWING: Figure 1

Description

Technical Field

[0001] The present invention relates to a cosmetic composition containing a eutectic mixture containing an alpha-hydroxy acid, an amino acid, and purified water; and a cosmetic composition containing a eutectic mixture containing a first amino acid and a second amino acid. More specifically, by including super purified water, a eutectic mixture of an amino acid, and an alpha-hydroxy acid, or a eutectic mixture of a first amino acid and a second amino acid, it has high skin permeability even under pH conditions with little skin irritation, is excellent in keratolytic effect and low-temperature stability, and maintains the keratolytic effect even under severe conditions.

Background Art

[0002] Alpha-hydroxy acid (α-hydroxy acid, hereinafter referred to as AHA) is one of the components commonly used to soften or remove the hyperkeratotic skin layer, and shows skin improvement effects such as promoting keratin turnover, collagen synthesis, enhancing skin moisturization, reducing fine wrinkles, treating and preventing acne (Korean Journal of Wellness, Vol. 10, No. 2, 2015, 161-169). Many AHAs are excellent in skin permeability and skin improvement effects under low pH conditions. However, due to severe skin irritations such as itching, tingling, and flushing, and various inflammatory irritations such as erythema and edema caused by low pH, it is difficult to use a high content. In addition, when AHA is dissolved in water, it ionizes, which has the disadvantage of slow skin absorption. In particular, when formulating AHA into a cosmetic, the pH of the dosage form becomes low, resulting in low dosage form stability and can have a negative impact such as irritation to the skin. To complement this, when raising the pH of the dosage form with a neutralizing agent, there is a problem that the effect of AHA decreases.

[0003] Furthermore, amino acids that exhibit exfoliating effects in dry skin along with the aforementioned AHAs are also widely used in topical cosmetics (Tatsuya Ozawa et al., The role of humectants in skin moisture retention. Skin Research 27, 276-288 (1985); and KR Registered Patent No. 10-1508168). However, the aforementioned AHAs and amino acids have a significant drawback in that their skin permeability decreases considerably at weakly acidic or neutral pH levels [SEWolverton, α-hydroxy acids, In Comprehensive Dermatologic Drug Therapy, 3 rd It is known through edition, Elsevier, 570 (2012), and at highly acidic pH levels, it can cause discomfort such as skin irritation, especially in people with sensitive skin.

[0004] On the other hand, the eutectic phenomenon is a phenomenon in which the melting point decreases when two or more substances are mixed. Each component in the mixture is in a solid state at room temperature, but when manufactured as a mixture, it does not crystallize due to van der Waals interactions or hydrogen bonding between the components, and becomes an aqueous solution that exists as a liquid at room temperature. Therefore, by selecting AHA as one of the components in the mixture, a liquid phase with water removed can be obtained. Since AHA does not ionize in the anhydrous state, it does not exhibit acidic properties, allowing AHA to be used with less irritation, and because AHA is not charged, it is very advantageous for skin penetration.

[0005] However, such eutectic mixtures have the problem of not being stable in cosmetics. When a protic solvent capable of hydrogen bonding is added to a composition containing a eutectic mixture, the solvent interacts with each component of the eutectic mixture (hydrogen bonding), interfering with the bonding between the components of the eutectic mixture, making it difficult to maintain the properties of the eutectic mixture. Furthermore, while anhydrous formulations are suitable for cosmetics containing eutectic mixtures, many eutectic mixtures have a specific gravity greater than that of water, leading to precipitation when mixed with oil and making uniform dispersion in the low-viscosity oil phase difficult. Conventionally, hydrophilic powders have been added to the composition to solve this problem, but this has resulted in the problem of hydrophilic eutectic mixtures adsorbing and agglomerating on the surface of the hydrophilic powders, making uniform dispersion difficult.

[0006] Therefore, against this background, the present inventors have researched and developed a cosmetic composition in which the eutectic mixture contains purified water, AHA, and amino acids; or two types of amino acids, while improving the irritation problem caused by low pH and dosage form stability, thereby completing the present invention. [Overview of the Initiative] [Problems that the invention aims to solve]

[0007] The inventors of the present invention conducted research to develop a cosmetic composition that addresses the problems of AHA causing irritation at low pH and the low skin permeability when AHA or amino acids are applied at skin pH. As a result, they confirmed that by adding purified water, AHA, and amino acids; two types of amino acids; or purified water and two types of amino acids in the form of a eutectic mixture to the cosmetic composition, the skin permeability of AHA or amino acids can be improved without skin irritation at a weakly acidic to neutral pH. Furthermore, the amino acids can be maintained stably and uniformly without precipitation even at low temperatures, and the exfoliating effect can be maintained even under harsh conditions (e.g., high temperature, high pressure), thus completing the present invention.

[0008] Therefore, the object of the present invention is to provide a cosmetic composition containing purified water, AHA and amino acids; two amino acids; or purified water and two amino acids in the form of a eutectic mixture, which exhibits excellent exfoliating effect even under weakly acidic to neutral conditions, is less irritating, has excellent low-temperature stability, and maintains its exfoliating effect even under harsh conditions. [Means for solving the problem]

[0009] As a means to solve the above problems, the cosmetic composition of the present invention is The present invention provides a cosmetic composition comprising purified water, amino acids, and alpha hydroxy acids (AHAs); or a eutectic mixture containing primary and secondary amino acids.

[0010] In one specific example, the cosmetic composition of the present invention comprises a eutectic mixture containing purified water; amino acids; and an alpha hydroxy acid (α-hydroxy acid, hereinafter referred to as AHA).

[0011] In the present invention, the alpha hydroxy acid (AHA) and amino acids may be included in the eutectic mixed water for use in skin improvement effects such as promoting exfoliation of the skin, improving pores, improving fine wrinkles, improving acne, or improving facial skin tone.

[0012] In this invention, the alpha hydroxy acid refers collectively to compounds in which an alcohol group or a hydroxyl group is added to the carbon at the α position of a carboxylic acid. It works by lowering the ionic bond energy of keratinocytes, thereby removing dead skin cells and promoting the formation of new cells, shortening the keratinization cycle which lengthens as the skin ages, and thereby promoting the activity of skin cells and improving the skin. In addition, AHAs can promote the production of collagen and elastin by fibroblasts, making the skin elastic and soft, and can also promote the production of mucopolysaccharides, which are important extracellular matrix components of the dermis, thereby increasing skin moisture. Due to the action of acid, AHAs help remove the connecting rings between keratinocytes in the stratum corneum, exhibiting an effect of smoothly dissolving dead skin cells. The aforementioned AHA may be one or more selected from the group consisting of lactic acid (found in spoiled milk and tomato juice), citric acid (found in mandarins and oranges), malic acid (found in apples), tartaric acid (found in wine), and glycolic acid and mandelic acid (found in sugarcane), but is not limited to these.

[0013] In one specific example, the AHA may be lactic acid or malic acid. Lactic acid has a low molecular weight among AHAs, allowing for rapid skin penetration and the rapid realization of the desired AHA effect. Malic acid, on the other hand, possesses multiple carboxyl groups, which can induce stronger bonding when producing eutectic mixtures. Its molecular structure is similar to that of amino acids, particularly serine, and it offers advantages in producing structurally stable dimer or multimer eutectic mixtures.

[0014] In one specific example of the present invention, when AHA is contained in the eutectic mixture, the AHA may be present in amounts of 1 to 50 parts by weight, for example, 5 to 40 parts by weight, 10 to 30 parts by weight, 15 to 20 parts by weight, 3 to 25 parts by weight, 5 to 20 parts by weight, or 6 to 18 parts by weight per 100 parts by weight of the eutectic mixture. If the AHA is present in less than 1 part by weight in the eutectic mixture, the desired effect cannot be achieved, and if it is present in amounts exceeding 50 parts by weight, skin irritation may occur.

[0015] As described above, when an AHA is contained in a eutectic mixture, the eutectic mixture can be produced by mixing the AHA with an amino acid that can bond to the AHA via polar intermolecular dipole-dipole attraction, dipole-induced dipole attraction, hydrogen bonding, or van der Waals interaction. In this case, the AHA can act as a hydrogen bond donor (HBD), and the amino acid can act as a hydrogen bond acceptor (HBA). For example, the amino acids may include alanine, cysteine, aspartic acid, glutamic acid, phenylalanine, glycine, histidine, isoleucine, lysine, leucine, methionine, asparagine, pyrrolysine, proline, glutamine, arginine, serine, threonine, selenocysteine, valine, tryptophan, or tyrosine.

[0016] In the present invention, the total amino acid content in the eutectic mixture may be, but is not limited to, 1 to 75 parts by weight, 5 to 70 parts by weight, 10 to 60 parts by weight, 15 to 50 parts by weight, 20 to 47 parts by weight, 10 to 70 parts by weight, 20 to 65 parts by weight, 30 to 60 parts by weight, 35 to 55 parts by weight, 40 to 50 parts by weight, or 42 to 47 parts by weight, based on 100 parts by weight of the entire eutectic mixture. If the eutectic mixture contains less than 1 part by weight of amino acids, the intended effect cannot be achieved, and if it contains more than 75 parts by weight, it may cause skin irritation and precipitation problems may occur because it does not dissolve in the composition. In one specific example, the amino acids contained in the eutectic mixture may be serine, proline, threonine, arginine, or cysteine.

[0017] In the present invention, the eutectic mixture can be produced from AHA and amino acids, and preferably from purified water, AHA, and amino acids.

[0018] In the present invention, the AHA and amino acids may be included in the eutectic mixture in a weight ratio of 1:0.1 to 1:10, for example, a weight ratio of 1:0.3 to 1:5, a weight ratio of 1:0.5 to 1:3, a weight ratio of 1:0.7 to 1:2, a weight ratio of 1:3 to 1:10, or a weight ratio of 1:5 to 1:8.

[0019] Generally, in the presence of purified water, AHAs in eutectic mixtures can ionize, leading to increased skin irritation. Therefore, with conventional eutectic mixtures, it was necessary to remove the solvent (purified water) through further steps such as evaporation, cooling, or freeze-drying. However, the eutectic mixture according to the present invention does not ionize in the presence of purified water through intermolecular bonding via dipole-dipole attraction, dipole-induced dipole attraction, hydrogen bonding, or van der Waals interactions between AHAs and amino acids, thus being safe from negative effects such as increased skin irritation.

[0020] The eutectic mixture according to the present invention contains purified water. The eutectic mixture of the present invention has a low melting point of 0°C or below due to the presence of purified water, which allows the amino acids in the composition to be stably maintained. Furthermore, the inclusion of purified water in the eutectic mixture allows the AHA and amino acids to be exposed to excess water, gradually dissociating their bonds, thereby exhibiting a skin-improving effect through the promotion of exfoliation. Therefore, the eutectic mixture of the present invention can maintain a uniform and stable liquid phase without the precipitation of amino acids even under various environmental changes.

[0021] In the present invention, the purified water may be present in an amount of 25 to 80 parts by weight per 100 parts by weight of the total eutectic mixture. If the purified water content in the eutectic mixture is less than 25 parts by weight, it does not reduce the melting point of the eutectic mixture, so amino acids will not be present stably. If it is present in an amount exceeding 80 parts by weight, the stability of hydrogen bonding is inhibited, reducing the skin permeability of AHA and amino acids, resulting in a low amino acid content, which may make it difficult to realize the desired effect. For example, the eutectic mixture of the present invention may contain purified water in amounts of 30 to 75 parts by weight, 35 to 70 parts by weight, 40 to 65 parts by weight, or 50 to 55 parts by weight based on the total weight of the eutectic mixture, but is not limited to these amounts.

[0022] In other specific examples, the cosmetic composition of the present invention comprises a eutectic mixture containing a first amino acid and a second amino acid.

[0023] In the present invention, the first and second amino acids may be one or more selected from the group consisting of alanine, cysteine, aspartic acid, glutamic acid, phenylalanine, glycine, histidine, isoleucine, lysine, leucine, methionine, asparagine, pyrrolysine, proline, glutamine, arginine, serine, threonine, selenocysteine, valine, tryptophan, and tyrosine. For example, the first and second amino acids may be two selected from the group consisting of serine, arginine, and glutamic acid.

[0024] In the above specific example, the total amino acid content in the eutectic mixture may be, but is not limited to, 1 to 75 parts by weight, 5 to 70 parts by weight, 10 to 60 parts by weight, 15 to 50 parts by weight, 20 to 47 parts by weight, 10 to 70 parts by weight, 20 to 65 parts by weight, 30 to 60 parts by weight, 35 to 55 parts by weight, 40 to 50 parts by weight, or 42 to 47 parts by weight, based on 100 parts by weight of the entire eutectic mixture. If the eutectic mixture contains less than 1 part by weight of amino acids, the desired effect cannot be achieved, and if it contains more than 75 parts by weight, it may cause skin irritation and precipitate because it does not dissolve in the composition. In this case, the first amino acid may be contained in an amount of 1 to 50 parts by weight based on 100 parts by weight of the entire eutectic mixture, and the second amino acid may be contained in an amount equal to 75 parts by weight minus the weight of the first amino acid. For example, the second amino acid may be 1 to 25 parts by weight. In the present invention, the first amino acid may be 1 to 50 parts by weight, preferably 5 to 40 parts by weight, 10 to 35 parts by weight, or 15 to 30 parts by weight.

[0025] In addition, the eutectic mixture of the present invention may further contain purified water. The purified water may be contained in an amount of 25 to 80 parts by weight based on 100 parts by weight of the entire eutectic mixture. The content of the purified water contained in the eutectic mixture can be directly applied to the content described above.

[0026] In the present invention, the eutectic mixture can be produced from a first amino acid and a second amino acid, and preferably can be produced from purified water, a first amino acid, and a second amino acid.

[0027] As described above, in the case of a eutectic mixture containing purified water, a first amino acid, and a second amino acid, it may contain 25 to 80 parts by weight of purified water, 1 to 50 parts by weight of the first amino acid, and 1 to 25 parts by weight of the second amino acid based on 100 parts by weight of the entire eutectic mixture.

[0028] In the following examples, it was confirmed that a eutectic mixture composed of two amino acids exhibits skin-improving effects such as reducing the number of pores, improving wrinkles, promoting skin regeneration, increasing the total amount of collagen, and enhancing skin elasticity, and that the above skin-improving effects are maintained even after storage under severe conditions such as high temperature. It was also confirmed that a eutectic mixture containing no AHA and containing only two amino acids and purified water exhibits excellent skin permeability, keratolytic effect, low-temperature stability, etc. under weakly acidic or neutral acidity conditions.

[0029] In the present invention, "eutectic mixture" means a mixture of two or more solid or liquid substances. By mixing two components having a high melting point, the two components form a complex through dipole-dipole attraction, dipole-induced dipole attraction, intermolecular hydrogen bonding, or van der Waals interaction between polar molecules. The complex formed as described above hinders the ability of each component to crystallize, that is, the ability to return to the solid state, and has a melting point lower than that of the component having the lowest melting point among the components, so that it can exhibit a liquid state at room temperature. In the present invention, since the eutectic mixture contains purified water, the lowest melting point is formed below zero, and a stable composition form can be maintained under various environments.

[0030] Furthermore, the eutectic mixture can be formed by the bonding of polar compounds. The polar compounds may be polar molecules or charged molecules. Also, there must be a difference in polarity between the polar compounds, and the same compound cannot form a eutectic mixture. More specifically, the pKa or pI values ​​between the components forming the eutectic mixture can differ by, for example, 1.0 or more, 2.0 or more, 3.0 or more, 4.0 or more, 5.0 or more, 6.0 or more, or 7.0 or more. In this case, if the components forming the eutectic mixture have multiple pKa values, they can be selected based on the pKa1 value.

[0031] More specifically, the polar compound can be selected from AHAs or amino acids (AAs). More specifically, if one of the components forming the eutectic mixture is an AHA, the other compound can be an amino acid having a pI value higher than that of the AHA. For example, the difference between the pKa1 and pI values ​​between the AHA and the amino acid forming the eutectic mixture may be 1.0 or higher, 2.0 or higher, 3.0 or higher, 4.0 or higher, 5.0 or higher, 6.0 or higher, or 7.0 or higher. More specifically, if one of the components forming the eutectic mixture is an amino acid, the other compound can be an amino acid having a pI value higher or lower than that of the amino acid. For example, the difference between the pI values ​​of two amino acids forming a eutectic mixture may be 1.0 or higher, 2.0 or higher, 3.0 or higher, 4.0 or higher, 5.0 or higher, 6.0 or higher, or 7.0 or higher.

[0032] More specifically, polar compounds can be selected from polar amino acids (polar AA), positively charged amino acids (+charged AA), and negatively charged amino acids (-charged AA). For example, one polar amino acid can form a eutectic mixture with one positively charged amino acid. For example, one polar amino acid can form a eutectic mixture with one negatively charged amino acid. For example, one positively charged amino acid can form a eutectic mixture with one negatively charged amino acid. Polar amino acids refer to any one of threonine, tyrosine, glutamine, serine, and asparagine. Positively charged amino acids refer to any one of histidine, lysine, and arginine. Negatively charged amino acids refer to any one of aspartic acid and glutamic acid.

[0033] The ratio of substances required for the eutectic mixture can be determined by the charge of the molecules of the components forming the eutectic mixture, the type and number of functional groups the molecules possess, and the polarity of the molecules or functional groups. Furthermore, the ratio of substances required for the eutectic mixture can be determined by the size and similarity of the molecules. For example, arginine and glutamic acid, arginine and aspartic acid, lysine and glutamic acid, lysine and aspartic acid, arginine and asparagine, and arginine and glutamine can form a eutectic mixture in a 1:1 molar ratio. For example, arginine and serine, arginine and threonine, arginine and tyrosine, arginine and asparagine, and arginine and glutamine can form a eutectic mixture in a 1:2 molar ratio.

[0034] In the present invention, the eutectic mixture can be used interchangeably with terms such as "eutectic solvent," "eutectic mixture," or "aqueous solution of eutectic mixture."

[0035] Generally, in the case of eutectic mixtures, the eutectic mixture is destroyed under harsh conditions such as high temperatures, reducing the effect exhibited by the eutectic mixture. When comparing the exfoliating effect of cosmetic composition A containing the eutectic mixture according to the present invention with cosmetic composition B after A has been exposed to high-temperature conditions for a certain period of time, A may be 5%, preferably 10%, more preferably 12%, and most preferably 15% or more higher than B. Furthermore, when comparing the skin permeability of cosmetic composition A containing the eutectic mixture according to the present invention with cosmetic composition B after A has been exposed to high-temperature conditions for a certain period of time, A may be 5%, preferably 10%, more preferably 12%, and most preferably 15% or more higher than B. In this case, the cosmetic composition exposed to high-temperature conditions for a certain period of time means that it has been stored at 50°C for 6 weeks or at 60°C for 5 weeks or more.

[0036] The eutectic mixture according to the present invention can have its acidity adjusted to a pH of 3.5 to 10 in order to improve discomfort such as skin irritation when applied to the skin. The eutectic mixture according to the present invention can be manufactured in a region where both the amine group of the amino acid or the carboxyl group of the AHA are charged, and this region can have a pH of 3.5 or higher. For example, the eutectic mixture can be adjusted to have a weakly acidic to neutral acidity such as pH 4 to pH 9, pH 5 to pH 8, or pH 6 to pH 7.

[0037] The eutectic mixture of the present invention, by containing purified water, AHA, and amino acids; or two types of amino acids, has a melting point depression property that reduces solubility at low temperatures, thereby increasing the low-temperature stability of the composition. Furthermore, conventional cosmetics have maintained a low pH because the skin permeability of AHA and / or amino acids generally decreases sharply as the pH increases. However, the eutectic mixture of the present invention can maintain high skin permeability of AHA and / or amino acids, as well as low pH, while reducing skin irritation that can occur at weakly acidic to neutral acidity.

[0038] The eutectic mixture according to the present invention can be produced by selecting any method commonly used by those skilled in the art, and then adding the step of adding water to the method, an example of a method for producing the eutectic mixture is as follows: i) A method of heating the component with the lowest melting point among the components forming a eutectic mixture to the temperature at which the component melts to create a liquid phase, and then completely dissolving the remaining components in the molten liquid. ii) A method of dissolving one or more species of each component constituting a eutectic mixture at a high temperature of 50°C or higher, so that they are completely dissolved in a solvent in which they dissolve and become homogeneous.

[0039] In this case, the solvent can be evaporated as needed using method ii).

[0040] In one specific example, the eutectic mixture of the present invention can be produced by the method of ii).

[0041] ii) As a specific example, the sample is prepared in a reaction vessel requiring heating and a homo-disper. The material of the reaction vessel is not limited; for example, a glass beaker or a reaction vessel made of SUS material can be used. To produce the eutectic mixture, in the case of amino acids-amino acids, serine and arginine are prepared in a molar ratio of 2:1, and in the case of amino acids-AHA, serine and malic acid are prepared in a molar ratio of 2:1. The amount of purified water may be 25 to 99 parts by weight based on the total weight of the prepared amino acids-amino acids or amino acids-AHA. Preferably, the amount of purified water is the same as the total weight of the amino acids-amino acids or amino acids-AHA, i.e., the purified water content is 50 parts by weight relative to the total weight. The speed of the homo-disper is preferably 600 to 4000 rpm, but may be higher or lower. For example, the homodisperser speed can be 600-3000 rpm, 600-2000 rpm, 600-1500 rpm, 800-1200 rpm, or 900-1100 rpm. Heating may be carried out at a temperature of 45-70°C, for example, 45-65°C, 45-60°C, or 45-55°C. The reaction time is preferably at least 15 minutes, and once homogeneity is confirmed, heating can be stopped and the mixture can be allowed to cool naturally.

[0042] Furthermore, as a method for determining the homogeneity of the eutectic mixture obtained as described above, i) A method for determining whether a transparent mixture has formed without precipitate when a eutectic mixture, which is an over-molten solution in which each component is dissolved beyond its solubility, is placed in front of a black background and illuminated with light, and ii) When a eutectic mixture is formed, a single melting point is formed at a temperature lower than the melting point of each component. Therefore, one method is to use a DSC (Differential Scanning Calorimetry) instrument to confirm the melting point.

[0043] When the AHA and / or amino acids are completely dissolved, the resulting mixture is clear and homogeneous, and maintains its liquid state and clarity even after a cooling process. The eutectic mixture produced as described above can maintain a clear liquid phase without phase separation, even at very low temperatures, such as below 0°C. Furthermore, the bonds of the eutectic mixture are maintained without being broken even after repeated solidification and melting processes.

[0044] The cosmetic composition according to the present invention may contain the eutectic mixture in amounts of 0.01 to 50 parts by weight, for example, 0.05 to 40 parts by weight, 0.1 to 30 parts by weight, 0.5 to 25 parts by weight, 0.1 to 50 parts by weight, 1 to 35 parts by weight, 5 to 30 parts by weight, 0.5 to 40 parts by weight, 1 to 30 parts by weight, 5 to 28 parts by weight, 8 to 25 parts by weight, 1 to 50 parts by weight, 5 to 40 parts by weight, 10 to 35 parts by weight, 15 to 25 parts by weight, 18 to 25 parts by weight, 15 to 20 parts by weight, 10 to 20 parts by weight, 10 to 30 parts by weight, 10 to 40 parts by weight, 15 to 35 parts by weight, or 20 to 30 parts by weight, relative to the total weight of the cosmetic composition, but is not limited to these amounts. The content of the eutectic mixture can be adjusted to suit the dosage form of the cosmetic composition.

[0045] In the following examples, it was confirmed that despite containing a high concentration of the eutectic mixture in the cosmetic, it exhibited low skin irritation and high skin permeability.

[0046] As described above, cosmetic compositions containing the eutectic mixture according to the present invention can exhibit skin-improving effects such as excellent exfoliation-promoting effects, pore reduction effects, elasticity-enhancing and wrinkle-improving effects, and skin regeneration-promoting effects.

[0047] The cosmetic composition according to the present invention may further contain an oil phase component. The oil phase component is included in the composition and present in the outer phase of the eutectic mixture, thereby helping the eutectic mixture to exist stably within the composition.

[0048] The oil phase component may be one or more oils or waxes. All oils and waxes commonly used as cosmetic ingredients in the art can be used. For example, the oils may be silicone oils, ester oils, triglyceride oils, hydrocarbon oils, or vegetable oils, and one or more of these components may be used in combination as needed.

[0049] For example, the silicone-based oil can be a silicone-based fluid oil or a silicone-based crosspolymer dispersed in the oil. For example, as the silicone-based fluid oil, you can use cyclopentasiloxane, cyclohexasiloxane, cycloheptasiloxane, cyclomethicone, cyclophenylmethicone, cyclotetrasiloxane, cyclotrisiloxane, dimethicone, caprylyl dimethicone, caprylyl trimethicone, caprylyl methicone, cetearyl methicone, hexadecyl methicone, hexyl methicone, lauryl methicone, myristyl methicone, phenyl methicone, stearyl methicone, stearyl dimethicone, trifluoropropyl methicone, cetyl dimethicone, diphenylsiloxy phenyl trimethicone, dimethylpolysiloxane, methylphenylpolysiloxane, decamethylcyclopentasiloxane, methyl trimethicone, or phenyl trimethicone. The aforementioned silicone-based oil component can be used alone or in combination with two or more other oils. As the silicone-based crosspolymer, dimethicone crosspolymer, dimethicone / vinyl dimethicone crosspolymer, dimethicone PEG-10 / 15 crosspolymer, or PEG-12 dimethicone / PEG-20 crosspolymer can be used, but are not limited to these.

[0050] Ester oils include ascorbyl palmitate, ascorbyl linoleate, ascorbyl stearate, diisostearyl malate, benzyl benzoate, benzyl laurate, butylene glycol dicaprylate / dicaplate, butylene glycol diisononanoate, butylene glycol laurate, butylene glycol stearate, butyl isostearate, cetearyl isononanoate, cetearyl nonanoate, cetyl caprylate, cetyl ethylhexanoate, cetyl isononanoate, ethylhexyl caprylate / caplate, ethylhexyl isononanoate, ethylhexyl isostearate, ethylhexyl laurate, hexyl laurate, octyldodecyl isostearate, isopropyl isostearate, isostearyl isononanoate, isostearyl isostearate, isocetyl ethylhexanoate You can use Neopentyl Glycol Dicaprate, Neopentyl Glycol Diethylhexanoate, Neopentyl Glycol Diisononanoate, Neopentyl Glycol Diisostearate, Pentaerythrityl Stearate, Pentaerythrityl Tetraethylhexanoate, Dipentaerythrityl Hexaacid Ester, Polyglyceryl-2 Diisostearate, Polyglyceryl-2 Sesquiisostearate, Polyglyceryl-2 Isostearate, Polyglyceryl-2 Tetraisostearate, Polyglyceryl-2 Triisostearate, Polyglyceryl-3 Diisostearate, Polyglyceryl-3 Isostearate, Polyglyceryl-4 Diisostearate, Polyglyceryl-4 Isostearate, Polyglyceryl-6 Diisostearate, Polyglyceryl-6 Sesquiisostearate, or Triethylhexanoin, etc.

[0051] Triglyceride-based oils that can be used include C8-C12 acid triglycerides, C12-C18 acid triglycerides, caprylic / capric / triglycerides, caprylic / capric / lauric triglycerides, C10-C40 isoalkyl acid triglycerides, C10-C18 triglycerides, glyceryl triacetyl hydrostearate, soybean triglycerides, tribehenin, tricaprine, triethylhexanoin, triheptanoin, triisostearin, tripalmitine, or tristearin.

[0052] Hydrocarbon-based oils that can be used include liquid paraffin (mineral oil), paraffin, petrolatum, microcrystalline wax, or squalene.

[0053] As for vegetable oils, you can use avocado oil, wheat germ oil, rosehip oil, shea butter, almond oil, olive oil, macadamia oil, argan oil, meadowfoam oil, sunflower oil, castor oil, camellia oil, corn oil, safflower oil, soybean oil, rapeseed oil, macadamia knot oil, jojoba oil, palm oil, palm kernel oil, or coconut oil.

[0054] The aforementioned wax can be any wax commonly used in cosmetics, such as hydrocarbon waxes, vegetable waxes, or silicone waxes. For example, it may include, but is not limited to, candelilla wax, carnauba wax, rice wax, beeswax, lanolin, ozokerite, ceresin wax, paraffin wax, microcrystalline wax, C30-C45 alkyldimethylsilyl polypropylsilsesquioxane, ethylene / propylene copolymer, or polyethylene wax.

[0055] The oil phase component is not particularly limited in content and may be present in the remaining amount after removing the eutectic mixture described above within the composition.

[0056] The cosmetic composition of the present invention may further contain, but is not limited to, all types of ingredients that can be used in ordinary cosmetics, such as humectants like glycerin, butylene glycol, propylene glycol, hexanediol, methyl gluceth-20, diglycerin, and ethylhexylglycerin; UV blockers like ethylhexyl methoxycinnamate, ethylhexyl salicylate, ethylhexyl triazone, octocrylene, and bis-ethylhexyloxyphenol methoxyphenyl triazine; pH adjusters like triethanolamine; thickeners like carbomer, xanthan gum, acrylate / C10-30 alkyl acrylate crosspolymer, and hyaluronic acid; preservatives like phenoxyethanol, methylparaben, and propylparaben; antioxidants like BHT, ethyl ascorbyl ether, and ascorbic acid; skin conditioning agents like beta-glucan; surfactants like cetearyl glucoacrylate and sorbitan stearate; and fragrances or dyes.

[0057] Each of the above-mentioned components contained in the cosmetic composition according to the present invention may preferably be included in the cosmetic composition according to the present invention within a range that does not exceed the maximum amount of use specified in the "Cosmetic Safety and Technical Specifications" established by the Chinese government.

[0058] The cosmetic composition according to the present invention can be manufactured in any dosage form commonly produced in the art. For example, the cosmetic composition may have dosage forms such as lotions such as softening lotions or nourishing lotions, spray-type lotions, facial lotions, emulsions such as body lotions, creams such as nourishing creams, moisturizing creams, and eye creams, sticks, essences, cosmetic ointments, sprays, gels, packs, sunscreens, makeup bases, foundations such as liquid or spray types, powders, makeup removers such as cleansing lotions and cleansing oils, and cleansing agents such as cleansing foams, soaps, and body washes, but is not limited to these.

[0059] In one specific example, the dosage form of the cosmetic composition may be a balm, water-in-oil (W / O), oil-in-water (O / W), solubilizing dosage form, or oil dosage form.

[0060] In the present invention, the "solubilizing formulation" refers to a formulation in which a small amount of oil component is transparently dissolved in water, and because the diameter of the oil drop is smaller than the wavelength of visible light, it does not interfere with the straight propagation of light without scattering or reflection, and exhibits transparent properties. The cosmetic composition having the solubilizing formulation may be a transparent skin, toner, hair tonic, or hair liquid, but is not limited to these types.

[0061] The cosmetic composition of the present invention can be used in the usual manner, and the number of times it is used can be varied depending on the user's skin condition or preference.

[0062] Furthermore, the present invention provides a method for producing the aforementioned cosmetic composition.

[0063] In one specific example, the cosmetic composition comprises the steps of mixing purified water, amino acids, and alpha hydroxy acids to produce a eutectic mixture, The process includes the step of producing a cosmetic composition containing the eutectic mixture.

[0064] In the above manufacturing method, the purified water, amino acids and alpha hydroxy acids, eutectic mixture and cosmetic composition can be applied as described above.

[0065] Furthermore, the step of producing the eutectic mixture may be carried out under homogenization conditions of 600 to 4000 rpm and 45 to 70°C. According to the present invention, by uniformly mixing the components that make up the eutectic mixture under the above-mentioned homogenization conditions, the skin-improving effect, low irritation to the skin, and excellent dosage form stability of the eutectic mixture can be realized.

[0066] In another specific example, the cosmetic composition comprises the steps of mixing a first amino acid and a second amino acid to produce a eutectic mixture, The process includes the step of producing a cosmetic composition containing the eutectic mixture.

[0067] Furthermore, the step of producing the eutectic mixture may also include a step of further including purified water.

[0068] In the above manufacturing method, the first amino acid, the second amino acid, purified water, the eutectic mixture, and the cosmetic composition can be applied as described above.

[0069] Furthermore, the step of producing the eutectic mixture may be carried out under homogenization conditions of 600 to 4000 rpm and 45 to 70°C. According to the present invention, by uniformly mixing the components that make up the eutectic mixture under the above-mentioned homogenization conditions, the skin-improving effect, low irritation to the skin, and excellent dosage form stability of the eutectic mixture can be realized.

[0070] The merits and features of the present invention, and the methods for achieving them, will become clearer with reference to the experimental and manufacturing examples described in detail below. However, the present invention is not limited to the experimental and manufacturing examples disclosed below, but can be embodied in a variety of different forms, which are provided merely to complete the disclosure of the present invention and to fully inform those who are ordinary skill in the art to which the present invention pertains. [Effects of the Invention]

[0071] The cosmetic composition according to the present invention contains alpha hydroxy acid (AHA), amino acids, and purified water; or two amino acids in the form of a eutectic mixture, thereby improving the skin permeability of AHA and / or amino acids without skin irritation at a weakly acidic to neutral pH, exhibiting excellent exfoliating effects, pore reduction effects, skin elasticity enhancement effects, and skin regeneration promoting effects, and can be maintained stably and uniformly even at low temperatures without amino acid precipitation. [Brief explanation of the drawing]

[0072] [Figure 1] Figure 1 shows the results of confirming the lowest melting point at -20°C due to the formation of a eutectic mixture. (A) shows the results for a serine aqueous solution containing a high amount of serine (Comparative Example 1), (B) shows the results for a mixture of serine and malic acid (Comparative Example 2), (C) shows the results for the eutectic mixture of the present invention (Example 1), (D) shows the results for a serine aqueous solution containing a low amount of serine (Comparative Example 3), and (E) shows the results for the eutectic mixture aqueous solution of the present invention (Example 2). [Figure 2] Figure 2 shows the results of confirming the degree of skin permeability with and without the formation of the eutectic mixture of the present invention. [Figure 3] Figure 3A shows the change in viscosity depending on the molar ratio of serine to arginine, and Figure 3B shows the change in conductivity depending on the molar ratio of serine to arginine. [Figure 4a] Figure 4a shows the NMR analysis results for serine (Ser) as A and for arginine (Arg) as B. [Figure 4b] Figure 4b shows the NMR analysis results of a eutectic mixture of serine and arginine, Figure D shows the NMR analysis results when citric acid is added to the eutectic mixture of serine and arginine, and Figure E shows the NMR analysis results after adding citric acid to the eutectic mixture of serine and arginine and storing it under harsh conditions (40°C) for one month. [Figure 5] Figure 5A shows the IR spectral analysis results for serine (Ser), Figure 5B shows the IR spectral analysis results for arginine (Arg), and Figure 5C shows the IR spectral analysis results for a eutectic mixture of serine and arginine. [Figure 6] Figure 6 shows the results of examining the effect of treatment with an essence (emulsion) containing a serine-arginine eutectic mixture on reducing the number of pores by age in participants aged 20-40. [Figure 7]Figure 7 shows the results of sensory evaluation (perceived effect evaluation) of the effect of treatment with an essence (emulsion) containing a serine-arginine eutectic mixture on reducing the number of pores in participants aged 20-40. [Figure 8] Figure 8 shows the results of measuring dermal density in participants aged 20-40 years after treatment with an essence (emulsion) containing a serine-arginine eutectic mixture. [Figure 9] Figure 9 shows the results of sensory evaluation (evaluation of perceived effect) of the increase in dermal density (increase in skin elasticity) in participants aged 20-40 years after treatment with an essence (emulsion) containing a serine-arginine eutectic mixture. [Figure 10] Figure 10 shows the results of treating HaCaT cells, a human-derived keratinocyte cell line, with an arginine-glutamic acid eutectic mixture to confirm its skin regeneration-promoting effect. [Modes for carrying out the invention]

[0073] The present invention will be described in detail below based on the following examples. However, the following examples are illustrative of the present invention, and the content of the present invention is not limited to the following examples.

[0074] Examples 1 and 2. Preparation of an aqueous solution of a eutectic mixture containing AHA and amino acids. The eutectic mixture aqueous solution was prepared by the following method.

[0075] The eutectic mixture of Example 1 was prepared by adding 18 parts by weight of malic acid (DL-malic acid, FUSO, Japan), which has a melting point of approximately 130°C, to 100 parts by weight of the eutectic mixture. The malic acid was heated to its melting point to produce a liquid phase. 20 parts by weight of serine (L-serine, EVONIK, Germany) was added to the liquid phase malic acid, and the mixture was stirred at 1,300 rpm for 30 minutes while maintaining the temperature to completely melt it. The completely melted liquid phase was neutralized with NaOH to a pH of 6.5. Thereafter, it was allowed to cool naturally at room temperature, and water was added using the Karl Fischer titration method until the total water content in the eutectic mixture was 53 parts by weight, and the mixture was stirred to produce a final mixture with a serine content of 20 parts by weight and a malic acid content of 18 parts by weight.

[0076] The eutectic mixture of Example 2 was prepared by adding 25 parts by weight of the eutectic mixture of Example 1 prepared above to purified water, based on 100 parts by weight of the total eutectic mixture. In this case, the aqueous solution contained 5 parts by weight of serine and 4.5 parts by weight of malic acid.

[0077] [Table 1]

[0078] Comparative Example 1. Production of a serine aqueous solution containing a high amount of serine. A serine aqueous solution was prepared by mixing 100 parts by weight of the total aqueous solution with purified water so that it contained 20 parts by weight of serine (L-Serine, EVONIK, Germany).

[0079] Comparative Example 2. Production of an aqueous solution of serine and malic acid containing high concentrations of serine and malic acid. Serine and malic acid were mixed in purified water, and the pH was adjusted to 6.5 using NaOH. The final aqueous solution was prepared so that, based on 100 parts by weight of the total mixture, it contained 20 parts by weight of serine (L-Serine, EVONIK, Germany) and 18 parts by weight of malic acid (DL-Malic acid, FUSO, Japan).

[0080] Comparative Example 3. Production of a serine aqueous solution containing a low amount of serine. A serine aqueous solution was prepared by mixing 100 parts by weight of the total aqueous solution with purified water so that it contained 5 parts by weight of serine (L-Serine, EVONIK, Germany).

[0081] Comparative Example 4. Production of an aqueous solution of serine and malic acid containing low concentrations of serine and malic acid. Serine and malic acid were mixed with purified water, and the pH was adjusted to 6.5 using NaOH. The final aqueous solution of the mixture was prepared by mixing purified water with 100 parts by weight of the total mixture, so that it contained 5 parts by weight of serine (L-Serine, EVONIK, Germany) and 4.5 parts by weight of malic acid (DL-Malic acid, FUSO, Japan).

[0082] Comparative Example 5. Production of Malic Acid Solution Malic acid aqueous solution was prepared by mixing 100 parts by weight of the total aqueous solution with purified water so that it contained 4.5 parts by weight of malic acid (DL-Malic acid, FUSO, Japan).

[0083] Examples 3 and 4. Preparation of a eutectic mixture aqueous solution containing two types of amino acids A eutectic aqueous mixture consisting of two types of amino acids was prepared by the following method.

[0084] The eutectic mixture of Example 3 was prepared by adding 21 parts by weight of arginine (L-arginine, Daesang, Republic of Korea), which has a melting point of approximately 220°C, to 100 parts by weight of the eutectic mixture, adding 26 parts by weight of serine (L-serine, EVONIK, Germany), then adding 10 parts by weight of purified water, and stirring at 1,300 rpm for 30 minutes while maintaining a temperature of 90°C until completely melted. The eutectic mixture of Example 4 was prepared by adding 6 parts by weight of hydrated citric acid (Citric acid, CIBA SPECIALITY CHEMICALS, Switzerland) for pH adjustment to the eutectic mixture of Example 3, and stirring at 1,300 rpm for 30 minutes while maintaining a temperature of 90°C until completely melted. Subsequently, the mixtures were allowed to cool naturally at room temperature, and water was added to the eutectic mixtures of Examples 3 and 4 using the Karl Fischer titration method until the total water content of the eutectic mixture reached 50-55 parts by weight, and the mixture was stirred. The pH values ​​of Examples 3 and 4 were 8.7 and 6.5, respectively.

[0085] [Table 2]

[0086] Experimental Example 1. Confirmation of melting point, low-temperature stability, and stability of eutectic mixture aqueous solutions. 1) Confirmation of melting point The properties of the eutectic mixture aqueous solution prepared in Example 1, the serine aqueous solution of Comparative Example 1, and the serine and malic acid aqueous solutions of Comparative Example 2 were compared based on their melting point depression. In this experiment, the melting points of the solutions of Example 1, Comparative Examples 1 and 2 were measured by observing the phase change after standing at -20°C. The results are shown in Figure 1. In Figure 1, A, B, and C represent Comparative Example 1, Comparative Example 2, and Example 1, respectively.

[0087] As is clear from Figure 1, in the case of the eutectic mixture formed with serine, malic acid, and purified water, as in Example 1, it was confirmed that the melting point was formed below -20°C and the liquid phase was maintained at -20°C. The aqueous solutions of the eutectic mixtures prepared in Examples 3 and 4 also underwent melting point depression due to the formation of the eutectic mixture, and it was confirmed that they had a melting point around -20°C or lower, which is lower than that of water, which has a melting point of 0°C.

[0088] 2) Confirmation of low-temperature stability To confirm the low-temperature stability of aqueous solutions to which the eutectic mixture was added, the aqueous solutions of Comparative Example 3 and Example 2 were frozen at a low temperature (-20°C) until completely solidified, and then thawed at room temperature (25°C). This process was repeated seven times, and the stability was visually confirmed. The results are shown in Figure 1, where D in Figure 1 is the result for the aqueous solution of Comparative Example 3, and E is the result for the aqueous solution of Example 2.

[0089] As is clear from Figures 1D and E, in the case of the serine aqueous solution of Comparative Example 3, serine precipitated due to a decrease in solubility at low temperatures, and it was confirmed that the precipitated serine settled after melting. However, it was confirmed that precipitation did not proceed in the aqueous solution containing a eutectic mixture, as in Example 2. Through this, it can be seen that aqueous solutions containing a eutectic mixture can maintain stability even at low temperatures such as -20°C.

[0090] Furthermore, it was confirmed that the eutectic mixture aqueous solutions prepared in Examples 3 and 4 either did not precipitate further or remelted after being left to stand at room temperature and then gently stirred. This indicates that even in Examples 3 and 4, which contain eutectic mixtures with two types of amino acids, stability is maintained at low temperatures such as -20°C.

[0091] 3) Confirmation of stability To confirm the stability of the eutectic mixture aqueous solutions, the serine aqueous solution of Comparative Example 1, which had the same serine content as Example 1, and the eutectic mixture aqueous solutions of Examples 1, 3, and 4 were left to stand in 50 ml test tubes at room temperature (25°C) and -20°C for 12 hours each, for a total of 4 weeks. The stability of the solutions was confirmed by the presence or absence of precipitation in the samples, and the results are shown in Table 3 below.

[0092] [Table 3]

[0093] As is clear from Table 3 above, despite repeated standing at room temperature and -20°C, the eutectic mixture aqueous solutions of Examples 1, 3, and 4 according to the present invention maintained their aqueous solution phase without sample precipitation, confirming that stability can be maintained even under diverse environmental changes. On the other hand, Comparative Example 1, which was a serine aqueous solution of the same concentration as Example 1, showed that the sample precipitated at -20°C.

[0094] Experimental Example 2. Exfoliating effect of eutectic mixture aqueous solution To confirm the exfoliating effect of the eutectic mixture aqueous solution at the serine concentration of Example 2, the aqueous solution was treated on the back skin of a pig. Specifically, a 6 mm diameter sample was taken from a 1 mm thick pig back skin using a biopsy tool. This was placed in a 96-well plate, washed once with phosphate-buffered saline (PBS), and then 100 μl of the eutectic mixture aqueous solution of Example 2 was added. At this time, in order to compare the exfoliating effect, 100 μl of the serine-malic acid mixture of Comparative Example 4 was added to the pig back skin using the same method. The pig back skin samples treated with each aqueous solution were stored for 16 hours at a temperature of 37°C and a humidity of 50%, and then the number of detached keratinocytes was measured with a cell counter to confirm the exfoliating effect. In this study, purified water was added as the negative control group, 10% gluconolactone (polyhydroxy acid, pHA) at pH 4, one of the keratin-exfoliating components, was added as the primary positive control group, and 10% gluconolactone at pH 6 was added as the secondary positive control group. For the secondary positive control group, the keratin-exfoliating effect was evaluated based on conditions that showed 15-20% efficacy compared to the primary positive control group. Here, the primary positive control group was used as a standard for comparing values ​​between actual experimental cases, and the relative value (%) of keratin exfoliation was calculated and recorded as [(Sample result value) / (Primary positive control group value) × 100]. The results are shown in Table 4.

[0095] [Table 4]

[0096] As is clear from Table 4 above, the results of Example 2 using the eutectic mixture aqueous solution of the present invention compared to the serine aqueous solution confirmed that a higher exfoliating effect was observed. Furthermore, when compared with 10% gluconolactone (PHA) (primary positive control group), which is an exfoliating component with a pH of 4, the aqueous solution of Example 2 of the present invention showed a similar level of exfoliating effect despite having an even higher pH value (pH 6.5). In other words, the aqueous solution of Example 2 of the present invention can exhibit an exfoliating effect at a similar level to or better than conventional exfoliating components with a low pH, even under mildly acidic or neutral pH conditions with minimal irritation. In addition, the increased exfoliating efficacy of the eutectic mixture aqueous solution of the present invention compared to the malic acid aqueous solution and a simple serine-malic acid mixture confirmed that eutectic mixtures containing purified water have even better exfoliating efficacy.

[0097] Experimental Example 3. Confirmation of skin permeability of eutectic mixture aqueous solution under weakly acidic conditions. The skin permeability of the eutectic mixture aqueous solution was confirmed under weakly acidic conditions (pH 6.5). 10 μg each of the aqueous solutions from Comparative Examples 1 and 2 and Example 1 were prepared and applied to a uniform area of ​​pig skin. A tissue moistened with phosphate-buffered saline (PBS) was placed on a 6-well plate, and the pig skin was placed on the tissue. The plate was stored at 37°C and 50% humidity for 12 hours. Thereafter, samples that did not penetrate the skin were removed using cotton swabs and keratin collection tape, and the weight of the pig skin was measured. After homogenizing the pig skin, 1 ml of water was added to thoroughly dissolve the amino acids and eutectic mixture in the sample, and then the plate was centrifuged at 12,000 rpm for 10 minutes. After centrifugation, 0.5 ml of the supernatant was separated, and the concentration of the sample (serine) that permeated the skin was quantified using the liquid chromatography method of the standard samples used in this experiment. The concentration was then divided by the weight of the pig skin, and the permeability was determined by comparing the mass of the sample (serine) per g of tissue with the initial mass of serine. The results of the skin permeability test are illustrated in Figure 2.

[0098] As is clear from Figure 2, under pH 6.5 conditions, Example 2 (the eutectic mixture in Figure 2) using the eutectic mixture aqueous solution of the present invention showed even higher porcine skin permeability compared to the serine aqueous solution (L-serine in Figure 2). Furthermore, as with Figure 2 and the references, Example 2 was found to exhibit superior skin permeability compared to lactic acid (Reference 1), a type of AHA, and salicylic acid (Reference 2), a type of beta-hydroxy acid (hereinafter referred to as BHA). Moreover, when compared to a simple serine-malic acid mixture (serine-malic acid in Figure 2), the eutectic mixture of the present invention containing purified water showed high porcine skin permeability. In other words, the high skin permeability of the eutectic mixture according to the present invention leads to the excellent exfoliating effect confirmed in Experimental Example 2 above.

[0099] [References] 1.SE Wolverton,α-Hydroxy acids.In Comprehensive Dermatologic Drug Therapy,3 rdet., Elsevier, 2012, 570. 2.SE Wolverton,α-hydroxy acids,In Comprehensive Dermatologic Drug Therapy,3 rd edition, Elsevier, 570 (2012)

[0100] Examples 5-7. Preparation of eutectic mixture aqueous solution Euclidean aqueous solutions of Examples 5-7 were prepared with the compositions and content shown in Table 5 below. First, malic acid (DL-Malic acid, FUSO, Japan), which has a melting point of 130°C, was prepared and heated to its melting point to produce a liquid phase. Proline (L-Proline, Sigma Aldrich, USA), threonine (L-Threonine, Sigma Aldrich, USA), and cysteine ​​(L-cysteine, Sigma Aldrich, USA) were added to the prepared liquid phase malic acid, and the mixture was stirred at 1,300 rpm for 30 minutes while maintaining the temperature to completely dissolve the substances. Once the solution was completely dissolved, it was neutralized to pH 6.5 using NaOH, then allowed to cool naturally at room temperature. The water content in the total eutectic mixture was measured using the Karl Fischer titration method to confirm the presence or absence of eutectic mixture formation and the presence or absence of precipitation of the sample after 4 weeks of storage. The results are shown in Table 6 below. In this case, the aqueous eutectic mixture aqueous solution with proline added is described in Example 5, the aqueous eutectic mixture aqueous solution with threonine added is described in Example 6, and the aqueous eutectic mixture aqueous solution with cysteine ​​added is described in Example 7.

[0101] [Table 5]

[0102] [Table 6]

[0103] Manufacturing Example 1. Manufacturing of a cosmetic composition in balm form. A balm-type cosmetic composition containing the eutectic mixture aqueous solution was prepared with the composition shown in Table 7 below. Specifically, the aqueous phase (purified water, 1,2-hexanediol, composition of Example 1) of the components listed below was uniformly dissolved at room temperature, and the oil phase (excluding the aqueous phase) of all components was uniformly dissolved at 90°C. After mixing with the aqueous phase, the mixture was cooled and solidified at room temperature to produce a balm-type cosmetic composition.

[0104] [Table 7]

[0105] Example 8 and Comparative Examples 6 and 7. Production of serine cosmetic compositions in oil-in-water formulations. Cosmetic compositions in an oil-in-water (O / W) dosage form containing a eutectic mixture or a serine aqueous solution were prepared according to the composition shown in Table 8 below. Specifically, the aqueous phase of the components listed below was uniformly dissolved at room temperature, and the oil phase was uniformly dissolved at 90°C. Thereafter, the dissolved aqueous phase was mixed with the oil phase and cooled to produce an oil-in-water cosmetic composition.

[0106] [Table 8]

[0107] Experimental Example 4. Confirmation of Skin Penetration Effect The skin penetration effect was confirmed using the following method with Example 8, Comparative Example 6, and Comparative Example 7, which are cosmetic compositions of the aforementioned oil-in-water dosage form.

[0108] 10 μg each of Example 8 and Comparative Examples 6 and 7 were prepared and applied to a uniform area of ​​pig skin. A tissue moistened with phosphate-buffered saline (PBS) was placed on a 6-well plate, and the pig skin was placed on the tissue. The plate was stored at 37°C and 50% humidity for 12 hours. Subsequently, samples that did not penetrate the skin were removed using cotton swabs and keratin collection tape, and the weight of the pig skin was measured. After homogenizing the pig skin, 1 ml of water was added to thoroughly dissolve the amino acids and eutectic mixture in the sample, and then the plate was centrifuged at 12,000 rpm for 10 minutes. After centrifugation, 0.5 ml of the supernatant was separated, and the concentration of the sample (serine) that permeated the skin was quantified using the standard liquid chromatography method used in this experiment. The concentration was then divided by the weight of the pig skin, and the permeability was determined by comparing the mass of sample (serine) per g of tissue with the initial mass of serine. The results are shown in Table 9.

[0109] [Table 9]

[0110] As can be seen from Table 9 above, in Example 8, skin permeability was improved by approximately 77% compared to Comparative Example 6, which contained the same amount of serine.

[0111] Manufacturing Example 2. Manufacturing of a water-in-oil (Water-in-Oil) formulation cosmetic composition. A water-in-oil (W / O) cosmetic composition containing an aqueous solution of a eutectic mixture was prepared with the composition shown in Table 10 below. Specifically, the aqueous phase of the components listed below was uniformly dissolved at room temperature, and the oil phase was uniformly dissolved at 90°C. Thereafter, the aqueous phase was mixed with the dissolved oil phase and cooled to produce a water-in-oil cosmetic composition.

[0112] [Table 10]

[0113] Example 9 and Comparative Example 8. Preparation of Oil-in-Water Formulation Cosmetic Compositions Cosmetic compositions in an oil-in-water (O / W) dosage form containing an aqueous solution of a eutectic mixture were prepared with the composition shown in Table 11 below. Specifically, the aqueous and oil phases of the following components were uniformly dissolved at room temperature, and then the oil phase was mixed with the dissolved aqueous phase to produce the oil-in-water cosmetic composition.

[0114] [Table 11]

[0115] Example 10 and Comparative Example 9. Preparation of cosmetic compositions in solubilizing form. A cosmetic composition in the form of a solubilizer containing an aqueous solution of a eutectic mixture was prepared with the composition shown in Table 12 below. Specifically, the aqueous phase and a small amount of oil phase from the components listed below were uniformly dissolved at room temperature, and thereafter, the small amount of oil phase was mixed with the dissolved aqueous phase to produce a cosmetic composition in the form of a solubilizer.

[0116] [Table 12]

[0117] Example 11 and Comparative Example 10. Preparation of oil-type cosmetic compositions An oil-type cosmetic composition containing an aqueous solution of a eutectic mixture was prepared with the composition shown in Table 13 below. Specifically, small amounts of the aqueous and oil phases of the components listed below were uniformly dissolved at room temperature, and then the dissolved small amount of aqueous phase was mixed with the oil phase to produce an oil-type cosmetic composition.

[0118] [Table 13]

[0119] Experiment Example 5. Confirmation of Skin Improvement Effect The cosmetic compositions of Example 9 and Comparative Example 8, manufactured as described above, were applied to the skin for 9 weeks, and the skin improvement effects (skin tone, skin texture, number of pores, and number of blackheads) were evaluated. The subjects' faces were divided in half by an imaginary line passing the nose, and the cosmetic composition of Example 9 was applied to the right side of the skin, while the cosmetic composition of Comparative Example 8 was applied to the left side of the skin. Subsequently, at weeks 3 and 9, the subjects compared the skin improvement effects on the right and left sides by looking in a mirror, and a sensory evaluation was performed using a 5-point scale. The results are shown in Table 14. In Table 14 below, at week 0, the score for each item of concern is shown using a 5-point scale. In order to eliminate external factors that change over time, such as season, individual physical condition, and mood, the change in satisfaction each week was not evaluated, and the efficacy of the eutectic mixture was evaluated by comparing only the condition of the right and left sides at each point in time.

[0120] [Evaluation Criteria for Skin Improvement Effects] <Week 0> 5 points: Skin condition is excellent for the relevant item, with no problems whatsoever. 4 points: Skin condition is relatively good for the relevant item, and there are not many concerns. 3 points: The skin condition is normal for the relevant item, but there are some minor concerns. 2 points: The skin condition in the relevant area is not good, and there are concerns. 1 point: The skin condition in the relevant area is very poor, causing significant concern. <Weeks 3 and 9> 5 points: The skin improvement effect on the right side is significantly better than on the left side. 4 points: The skin improvement effect on the right side is superior to that on the left side. 3. It is difficult to notice the improvement in skin on the right and left sides. Points 2: The skin improvement effect on the left side is superior to that on the right side. 1 point: The skin improvement effect on the left side is significantly better than on the right side.

[0121] [Table 14]

[0122] In the case of weeks 3 and 9, with a score of 3 as the average, a higher score indicates that consumers feel a greater improvement in the skin on the right side where the eutectic mixture was applied. Thus, as is clear from Table 14 above, it can be confirmed that when the cosmetic composition of the present invention is applied for 9 weeks, a skin improvement effect is felt, and in particular, skin problems that consumers find difficult to perceive as effective, such as blackheads, pore count, and black pores, are dramatically improved.

[0123] Experiment Example 6. Confirmation of enhanced perceived effect with wash-off type. The cosmetic compositions of Examples 10 and 11 and Comparative Examples 9 and 10, manufactured as described above, were applied to the skin, washed with lukewarm water, and the perceived improvement effect of short-term application using a wash-off type was evaluated. On day 1, subjects were instructed to use one product randomly selected from either Example 10 or Comparative Example 9, and on day 2, they were instructed to use the other product from either Example 10 or Comparative Example 9 that was not selected on day 1. On both days, the change in perceived effect before product application and after washing the product with lukewarm water was evaluated on a 5-point scale after a blind test. In this evaluation, the inventor, who conducted the experiment, knew which product the subject had selected, and the subject proceeded in a blind test until completing the questionnaire. Example 11 and Comparative Example 10 were also evaluated in the same manner as described above. Through the above experiment, it was confirmed whether consumers perceive short-term efficacy with or without the eutectic mixture in two different dosage forms: a solubilized dosage form (Example 10 and Comparative Example 9) and an oil dosage form (Example 11 and Comparative Example 9). The results are shown in Tables 15 and 16, respectively.

[0124] [Evaluation Criteria for Skin Improvement Effects] ○: Excellent perceived skin improvement effect △: Normal perceived skin improvement effect ×: No noticeable improvement in skin condition.

[0125] [Table 15]

[0126] [Table 16]

[0127] As is clear from Tables 15 and 16 above, sensory evaluation confirmed that in the case of the cosmetic composition of the present invention, the overall perceived skin improvement effect increases after use.

[0128] Example 12. Preparation of a eutectic mixture containing serine and arginine. A serine:arginine mixture was prepared in a molar ratio of 2:1 to form the eutectic mixture, and an equal amount of purified water was prepared to match the total weight. That is, the amount of purified water was adjusted so that its content was 50 wt% of the total weight. The mixture was heated to maintain a homodisper rate of 1000 rpm and a temperature of 50°C. After reacting for 20 minutes, once homogeneity was confirmed, heating was stopped and the mixture was allowed to cool naturally.

[0129] Experimental Example 7. Confirmation of the properties of a eutectic mixture containing serine and arginine. 1) Confirmation of the physical properties of the eutectic mixture During the preparation of the eutectic mixture in Example 12, the properties and long-term stability of the composition were compared based on the molar ratio of serine to arginine. The specific molar ratios, properties, and long-term stability are shown in Table 17 below.

[0130] [Table 17]

[0131] As is clear from Table 17 above, when serine and arginine are mixed in a molar ratio of 2:1, it was confirmed that the mixture is stable and does not precipitate even after 2 months of storage. This indicates that serine and arginine form a eutectic mixture when they are present in a molar ratio of 2:1.

[0132] 2) Confirmation of viscosity and conductivity of the eutectic mixture The viscosity and conductivity of the compositions based on the molar ratio of serine and arginine shown in Table 17 were confirmed and are shown in Figure 3.

[0133] First, looking at Figure 3A, which shows the viscosity, we can see that the viscosity is highest at a 2:1 molar ratio, which forms a eutectic mixture. This is because dissolution beyond the solubility limit occurs in the eutectic mixture, resulting in an over-dissolution state and increased viscosity. Furthermore, looking at the conductivity results (Figure 3B), we can see that when serine and arginine have a 2:1 molar ratio, a eutectic mixture is formed and conductivity decreases due to the stabilization of the surface charge (i.e., improved affinity to the surface).

[0134] 3)NMR analysis For NMR analysis of the eutectic mixture, NMR analyses of serine and arginine were performed separately (Figure 4a, A and B). Furthermore, NMR analyses of the serine-arginine eutectic mixture (Figure 4b, C), the serine-arginine eutectic mixture to which citric acid was added as a pH adjuster (Figure 4b, D) which has a strong surface charge that affects the bonding within the dosage form and can cause the cosmetic dosage form to exhibit weak acidity, and the NMR analysis results after adding citric acid to the eutectic mixture and storing it under harsh conditions (40°C) for one month (Figure 4b, E) are shown in Figures 4a and 4b.

[0135] As a result, it was confirmed that the single peak observed in the NMR analysis of arginine (Figure 4a, B) was split into two peaks when formed in the serine-arginine eutectic mixture. In other words, a split was observed in the serine-arginine eutectic mixture between the hydrogen peak of the main chain closest to the guanidine of arginine through intermolecular bonding. This can be interpreted as the rotational motion of the main chain being inhibited, leading to intermolecular bonding. Furthermore, when NMR analysis was performed after adding citric acid to the serine-arginine eutectic mixture (Figure 4b, D), the same peaks were observed in the serine-arginine eutectic mixture despite the condition that the eutectic mixture could be destroyed by the addition of citric acid. The analysis results remained the same even after storing it under harsh conditions (40°C) for one month (Figure 4b, E). Through this, it was demonstrated that the serine-arginine eutectic mixture of the present invention can be stably maintained even under harsh conditions.

[0136] 4) IR spectral analysis The IR spectra of serine and arginine individually (A and B in Figure 5) and the serine-arginine eutectic mixture were analyzed (Figure 5). As a result, a unique red shift was observed in the IR spectrum due to the formation of a eutectic mixture between serine and arginine. This indicates that the guanidine group of arginine and the carboxyl group of serine are red-shifted, and that the red shift occurs because the intramolecular bond is loosened by the intermolecular bond (i.e., the bond length increases). Therefore, through the results of the above structural analysis, the intermolecular bond of the eutectic mixture was confirmed, and its structural stability was verified.

[0137] Experimental Example 8. Confirmation of Skin Improvement Effect 1) Confirmation of the effect of reducing the number of pores Sixty subjects aged 20-40 applied an essence (emulsion) containing a eutectic mixture of serine and arginine prepared in Example 12 to their skin. After 6 weeks, the number of pores was measured to confirm the effect of reducing pore count. In this study, the reduction in pore count was defined as the number of pores recognized by the device; a reduction was determined when the size, color, and visibility of individual pores decreased to the point where the device no longer recognized them as pores. The device used was the Antera 3D® CS skin analyzing camera (Miravex). The same evaluator maintained the same skin conditions by adapting the subjects' skin to a constant temperature and humidity environment for 20 minutes, and then measured and evaluated the pore count at the same points on both cheeks. Therefore, the reduction in pore count refers to an improvement in enlarged pores. The results for each age group are shown in Figure 6 and Table 18 below.

[0138] [Table 18]

[0139] As is clear from Table 18 and Figure 6 above, a sustained reduction in the number of pores was observed in the skin of experimental participants of all age groups that were treated with an essence (emulsion) containing a serine-arginine eutectic mixture.

[0140] 2) Sensory evaluation of the reduction in the number of pores Furthermore, a sensory evaluation was conducted on the reduction in the number of pores by using the essence containing the serine and arginine eutectic mixture of Example 12. The results are shown in Figure 7 below.

[0141] Similar to the results of 1), we were able to confirm that even more participants showed a superior pore reduction effect in the essence treatment group containing a eutectic mixture of serine and arginine.

[0142] 3) Confirmation of elasticity improvement effect - Confirmation of dermal density Using the same method as in 1), the improvement effect on dermal density by age group in participants aged 20-40 was confirmed and is shown in Figure 8.

[0143] As is clear from Figure 8, it was confirmed that the experimental group treated with the essence containing a eutectic mixture of serine and arginine showed high dermal density in all age groups. In other words, this indicates that treatment with the serine-arginine eutectic mixture can improve elasticity by increasing dermal density.

[0144] 4) Sensory evaluation of elasticity improvement Furthermore, a sensory evaluation was conducted on the increase in dermal density achieved by using the essence containing the serine and arginine eutectic mixture of Example 12. The results are shown in Figure 9.

[0145] Similar to the results in 3), we were able to confirm that even more participants in the experimental group treated with an essence containing a eutectic mixture of serine and arginine showed a superior effect in increasing dermal density.

[0146] Comparative Example 11. Preparation of a simple mixture of serine and arginine To 100 parts by weight of the mixture, 5 parts by weight of serine and 4.1 parts by weight of arginine were added to 90.5 parts by weight of purified water, and the mixture was simply mixed using a homodispo until no precipitate remained. Simple mixing means mixing at room temperature without any additional heating step.

[0147] Experimental Example 9. Exfoliating effect of eutectic mixtures To confirm the exfoliating effect of the serine-arginine eutectic mixture of Example 12 and the simple mixture of serine and arginine of Comparative Example 11, the eutectic mixture and the simple mixture were treated on the skin of a pig's back. The specific experimental method was the same as in Experimental Example 2. The results are shown in Table 19 below.

[0148] [Table 19]

[0149] As is clear from Table 19 above, it was confirmed that the serine-arginine eutectic mixture of the present invention exhibits an even higher exfoliating effect compared to a simple mixture of serine and arginine. When compared with the primary positive control group, similar to Experimental Example 2, it was confirmed that the eutectic mixture of the present invention exhibited an exfoliating effect at a similar level to that of PHA, despite having an even higher pH value (pH 9.1). Furthermore, it was confirmed that the group in which the pH was adjusted to 6.0 with citric acid also exhibited an exfoliating effect at a similar level to that of the eutectic mixture before adjustment.

[0150] Experimental Example 10. Confirmation of Skin Permeability The skin permeability of the eutectic mixture prepared in Example 12 was confirmed. The specific experimental method was the same as in Experimental Example 3. The results for Example 12 are shown in Table 20.

[0151] [Table 20]

[0152] When the pH of the eutectic mixture according to the present invention was adjusted to 6.0 by adding citric acid, the eutectic mixture of Example 12 according to the present invention exhibited excellent skin permeability even under conditions of pH 9.1 without pH adjustment. On the other hand, the simple mixture of serine and arginine showed significantly lower skin permeability than Example 12, and even when the pH was adjusted to 6.0, it showed lower results compared to the eutectic mixture according to the present invention.

[0153] Example 13. Preparation of a eutectic mixture containing arginine and glutamic acid. Arginine and glutamic acid were prepared in a molar ratio of 1:1 as components to form the eutectic mixture, and the same amount of purified water as the total weight was prepared. That is, the amount of purified water was such that the purified water content was 50 wt% of the total weight. The mixture was heated so that the homodisper rate was maintained at 1000 rpm and the temperature at 50°C. After reacting for 20 minutes, once homogeneity was confirmed, heating was stopped, and the mixture was allowed to cool naturally to produce the eutectic mixture in the same manner as in Example 12.

[0154] Experimental Example 11. Confirmation of the properties of a eutectic mixture containing arginine and glutamic acid. A macroscopic evaluation of eutectic mixture formation between arginine and glutamic acid was conducted. The solubility of glutamic acid, simple arginine / glutamic acid mixtures, and arginine-glutamic acid eutectic mixtures in water was confirmed, and the ability to dissolve at a concentration of 1% or more is shown in Table 21 below.

[0155] [Table 21]

[0156] As a result, as shown in Table 21 above, the solubility in water for glutamic acid and simple mixtures of arginine / glutamic acid was very low, less than 0.5%, and it was difficult to dissolve them at a concentration of 0.5% or more in actual dosage forms such as oil and aqueous phases. On the other hand, the eutectic mixture of arginine-glutamic acid had a solubility in water of 15.2%, and it was confirmed that it could exist in the aqueous solution phase and that further dissolution was possible through eutectic bonding.

[0157] Experimental Example 12. Confirmation of the effect of promoting skin regeneration. HaCaT cells, a human-derived keratinocyte cell line, were cultured at 37°C and 5% CO2 in DMEM medium (ADDEXBIO TECHNOLOGIES, San Diego, CA, USA) supplemented with 10% fetal bovine serum (Gibco, Waltham, MA, USA), 100 mg / ml penicillin, and 100 mg / ml streptomycin. The cultured cells were divided into 2.0 × 10⁶ wells. 5After seeding the cells into a 24-well plate, they were cultured for 24 hours. The cells were then washed once with PBS, and a vertical linear scratch was created in the middle of the well by scraping the cells with a 200 μl pipette tip. To remove the detached cell debris, the wells were washed twice with PBS, and the medium was replaced with PBS-free DMEM containing each test substance. To ensure consistent imaging, the center of each well was marked with a fine marker, and a photograph of the marked area was taken at the start of the experiment (0 hours). Similarly, photographs were taken at 2, 4, and 24 hours. The scratch area created on the single layer of cells was measured using the Image J image analysis program for the 0-hour and 24-hour photographs. The area where the scratch area was filled was then expressed as the wound healing percentage (%) using the following general formula 1. The results are shown in Table 22 and Figure 10 below. In this case, the concentration of the Arg / Glu (10:1) simple mixture was based on the arginine concentration.

[0158]

number

[0159] [Table 22]

[0160] As shown in Table 22 and Figure 10 above, using FBS-free DMEM medium as the negative control group and 10% FBS-DMEM, which corresponds to the optimal growth conditions for the cell line, as the positive control group, we compared an arginine-glutamic acid eutectic mixture (Arg-Glu eutectic mixture) with the single amino acids arginine and glutamic acid, and simple mixtures of the two amino acids. The results showed that the group treated with 1 ppm Arg-Glu eutectic mixture had a better wound healing rate than the group treated with 10% FBS (positive control group). Furthermore, the wound healing rate for the Arg-Glu eutectic mixture at 10 ppm (based on arginine concentration) was 92.2%, and microscopic images confirmed that the spaces previously left open by scratches were largely covered with cells. The effect of the 10 ppm Arg-Glu eutectic mixture was superior to that of the treatment group using a simple mixture of the two amino acids, 10 ppm arginine / 1 ppm glutamic acid (the maximum achievable glutamic acid concentration is 1 ppm), and the treatment group using 1 ppm arginine. Through this, we confirmed that the Arg-Glu eutectic mixture can promote cell regeneration and aid in skin regeneration compared to when each is applied individually or simply mixed.

[0161] Experimental Example 13. Confirmation of the effect of increasing total collagen volume. To confirm the effect of the eutectic mixture of Example 13 on increasing the total amount of collagen, human fibroblasts were treated with an arginine-glutamic acid eutectic mixture, and the total amount of collagen produced by the human fibroblasts was measured. Specifically, an arginine-glutamic acid eutectic mixture (Arg-Glu eutectic mixture), arginine (Arg), glutamic acid (Glu), and a simple arginine-glutamic acid mixture (Arg / Glu simple mixture) were added to the culture medium of human fibroblasts, and the degree of increase in total collagen at the cellular level was confirmed. The total amount of collagen was quantified using the PICP EIA kit (Procollagen Type I C-Peptide Enzyme ImmunoAssay KIT). Before the experiment, the cytotoxicity of the Arg-Glu eutectic mixture was evaluated at different concentrations (μg / ml) using human-derived fibroblasts, and a concentration that did not cause cytotoxicity (100 μg / ml) was selected to evaluate the degree of increase in total collagen.

[0162] Specifically, each sample was added to human fibroblast culture medium and cultured for one day. After the culture medium was taken, the increase in total collagen at each concentration was measured at 450 nm using a spectrophotometer with a PICP EIA kit. To compare the effects, the increase in total collagen was confirmed using the same method for fibroblast culture medium with no additives (negative control group) and for samples to which TGF-βfmf was added to a final concentration of 10 mg / ml (positive control group). The total amount of collagen was measured as UV absorbance, and the increase rate of total collagen was calculated as the relative ratio of total collagen to the control group. The results are shown in Table 23 below. In this case, the concentrations of the Arg-Glu eutectic mixture and Arg / Glu (1:10) simple mixture were based on the concentration of arginine.

[0163] [Table 23]

[0164] As is clear from Table 23 above, the Arg-Glu eutectic mixture showed the effect of promoting collagen synthesis in a concentration-dependent manner and increasing the total amount of collagen. At concentrations of 1 ppm or higher, the collagen increase rate was better than that of the same concentration of Arg / Glu (1:10) simple mixture. Furthermore, it was confirmed that the Arg-Glu eutectic mixture showed a better collagen increase rate compared to the single amino acids Arg and Glu.

[0165] Experimental Example 14. Confirmation of the effect on increasing skin elasticity. To confirm the elasticity-enhancing effect of the eutectic mixture of Example 13 on actual human skin, A and B were prepared in cream form with the compositions shown in Table 24 below, and experiments were conducted. This experiment used an arginine-glutamic acid eutectic mixture (Arg-Glu eutectic mixture). Specifically, 20 women aged 25-45 years applied the mixture to their faces twice daily, morning and evening, and the effect of improving skin elasticity was measured using a skin elasticity measuring instrument (Cutometer SEM 575, C+K Electronic Co., Germany). The results are shown in Table 25 below. The result values ​​represent the viscoelasticity of the skin measured by the skin elasticity measuring instrument.

[0166] [Table 24]

[0167] [Table 25]

[0168] As is clear from Table 25 above, skin elasticity was confirmed to increase in the experimental group treated with the eutectic mixture of arginine and glutamic acid. This indicates that the eutectic mixture of arginine and glutamic acid exhibits an excellent effect in enhancing skin elasticity.

[0169] Experimental Example 15. Confirmation of Separation Conditions for Euclidean Mixtures Generally, when a eutectic mixture is formed with a specific solvent, the eutectic mixture is placed in a state of over-moltenness beyond the solubility of each component. In the present invention, in the case of an over-molten solution formed using water as the solvent, the dissociation of the bonds in the eutectic mixture can be confirmed by the presence or absence of irreversible precipitate formation under conditions such as the following: i) When stored at a temperature of 50°C for 6 weeks or more, or 8 weeks or more, or 10 weeks or more, or 12 weeks or more; ii) When stored at a temperature of 60°C for 4 weeks or more, or 5 weeks or more, or 6 weeks or more, or 8 weeks or more; and / or iii) When stored at a temperature of 80°C for 10 days or more, or 15 days or more, or 20 days or more, or 30 days or more.

[0170] In other words, under the conditions i) to iii) above, the presence or absence of irreversible precipitate formation can be used to break the bonds between eutectic substances in the eutectic mixture, and this breakdown can be confirmed. Furthermore, the presence or absence of dissociation of the eutectic mixture can be confirmed using a substance that can break the bonds between eutectic substances, such as EDTA, a protic solvent, a high concentration of urea, or guanidyl HCl.

[0171] In this context, reversible precipitation refers to a precipitate of a eutectic mixture that, after being observed, returns to a clear (homogeneous) solution state after approximately 10 to 20 simple mixing (hand shaking). When left to stand at a low temperature (-20°C) that does not meet the dissociation conditions described in i) to iii) above, it was confirmed that a precipitate temporarily occurs, but that it becomes homogenized through simple mixing. Irreversible precipitation refers to a precipitate that does not become homogenized when the above process is carried out.

[0172] Furthermore, the properties of the cosmetic (essence) containing the eutectic mixture from Example 12, which was allowed to stand under the separation conditions for the eutectic mixture, were observed visually (Table 27), and the melting point change (Table 28) and exfoliating effect (Table 29) were confirmed through DSC (Differential scanning calorimetry) measurement to confirm whether or not the eutectic mixture had dissociated. In this case, the essence containing the eutectic mixture means that the eutectic mixture was added to the essence manufactured with the composition shown in Table 26 below, and the exfoliating effect was measured in the same way as described in Experimental Example 2. In this case, the melting point was measured using a Perkin Elmer Diamond DSC (Differential Scanning Calorimeter; Perkin Elmer, Waltham, MA, USA). The melting point is indicated by the heat flow to the sample in the DSC, and its accuracy is known to be 0.01°C. The melting point measurement was performed in a pure nitrogen atmosphere, which was supplied into the measurement chamber at a rate of 20 cc / min to maintain the nitrogen atmosphere, and the chamber temperature was set to increase by 10°C per minute. The mass of the sample to be measured was confirmed by subtracting the weight of the pan used to load the sample into the DSC from the total weight including the sample. The pan was left to stand inside the DSC, and the heat flow was measured by heating it with the temperature set from -50°C to 90°C. Thereafter, the results were confirmed through a data analysis program connected to the DSC.

[0173] [Table 26]

[0174] [Table 27]

[0175] As is clear from Table 27 above, when the mixture was left to stand for more than 6 weeks at a temperature of 50°C or more than 5 weeks at a temperature of 60°C, it was confirmed that the eutectic mixture had dissociated through the formation of irreversible precipitates. When the eutectic mixture dissociates, the effects of melting point depression and improvement of skin penetration efficacy cannot be expected, so the characteristics of the eutectic mixture when it is formed or dissociated were confirmed through Tables 28 and 29 below.

[0176] [Table 28]

[0177] As a result, the melting point of cosmetics containing a simple mixture that does not form a eutectic mixture was observed to be 0°C, the melting point of water. However, the melting point of cosmetics containing a eutectic mixture was observed to be -3.83°C due to the melting point depression phenomenon. In the case of cosmetics containing a eutectic mixture stored under the dissociation conditions in Table 27 (6 weeks at 50°C), the melting point was observed to be 0°C, confirming that the eutectic mixture dissociated into the form of a simple mixture.

[0178] [Table 29]

[0179] When the eutectic mixture identified in Table 28 dissociated into a simple mixture, we checked whether the level of exfoliation effect was similar to that of the simple mixture using Table 29. When comparing the exfoliation effect of the essence containing the eutectic mixture with a baseline of 100, we confirmed that all essences containing the simple mixture or the dissociated eutectic mixture showed similar levels of effectiveness. In this case, the essence containing the eutectic mixture in [Example 12] showed an exfoliation effect that was approximately 20% higher than that of the essence containing the eutectic mixture in [Example 12] after storage at 50°C for 6 weeks (under severe conditions) (including the dissociated eutectic mixture).

[0180] As is clear from Tables 28 and 29 above, when comparing the melting point and exfoliating effect of the essence containing the eutectic mixture and the essence containing the dissociated product of the eutectic mixture, it was confirmed that the dissociated product of the eutectic mixture exhibited a melting point and exfoliating effect similar to that of the essence containing the simple mixture. In other words, the above results demonstrate that it is possible to confirm the presence or absence of dissociation of the eutectic mixture.

Claims

1. A cosmetic composition comprising a eutectic mixture containing arginine and glutamic acid.

2. The cosmetic composition according to claim 1, wherein the arginine and glutamic acid are contained in a total amount of 1 to 75 parts by weight per 100 parts by weight of the entire eutectic mixture.

3. The cosmetic composition according to claim 1, wherein the eutectic mixture further comprises purified water.

4. The cosmetic composition according to claim 3, wherein the purified water is contained in an amount of 25 to 80 parts by weight per 100 parts by weight of the entire eutectic mixture.

5. The cosmetic composition according to claim 1, wherein the eutectic mixture is contained in an amount of 0.01 to 50 parts by weight per 100 parts by weight of the total composition.

6. The cosmetic composition according to claim 1, wherein the cosmetic composition has a pH value of 3.5 to 10.

7. The cosmetic composition according to claim 1, wherein the cosmetic composition is for improving pores by reducing the number of pores.

8. The cosmetic composition according to claim 1, wherein the cosmetic composition is for the purpose of increasing elasticity.

9. The cosmetic composition according to claim 1, wherein the cosmetic composition is for skin regeneration.

10. The cosmetic composition according to claim 1, wherein the cosmetic composition is for the purpose of improving wrinkles.

11. The cosmetic composition according to claim 1, wherein the cosmetic composition is for improving exfoliation of the stratum corneum.

12. A step of mixing arginine and glutamic acid to produce a eutectic mixture, A method for producing a cosmetic composition, comprising the step of adding the eutectic mixture to produce the cosmetic composition.

13. The method for producing a cosmetic composition according to claim 12, wherein the step of producing the eutectic mixture is carried out under homogenization conditions of 600 to 4000 rpm and 45 to 70°C.

14. The method for producing a cosmetic composition according to claim 12, wherein the step of producing the eutectic mixture further includes a step of producing purified water.

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