MULTI-WAVELENGTH IN-LINE URINE ANALYSIS SYSTEM AND METHOD

Abstract

The invention relates to a multi-wavelength inline urine analysis system and method that enables the measurement of urine passing through a catheter or drainage line in the ultraviolet, visible and near-infrared spectral regions by means of detection by an optical detection module (6) through a sample chamber (4) containing urine emitted from an LED light source (1), and the evaluation of the obtained data together with multivariate calibration and analysis software (8) to determine multiple urine parameters and transfer the measurement results to the user interface and data logging unit (9), thereby reducing the use of reagents and the need for sampling, and allowing for continuous or interval monitoring of urine inline.

Description

1 TARIFF MULTI-WAVELENGTH IN-LINE URINE ANALYSIS SYSTEM AND METHOD Technical Area 5 The invention is for biomedical optical measurement, in vitro diagnostics (IVD), and bedside continuous monitoring. These systems are related to technical fields, particularly regarding urine samples or catheters or The urine flow passing through the drainage line is measured in-line using ultraviolet, visible, and Multi-wavelength optical measurement in the near-infrared (UV–Vis–NIR) spectral regions 10 by analyzing the absorbance, transmission, scattering and When necessary, multiple urine-related analyses can be performed by evaluating fluorescence signals together. Multi-wave technology for the qualitative or quantitative determination of biochemical parameters. It relates to inline urine analysis systems and methods. The Invention's Infrastructure Urine analysis; evaluation of kidney and urinary system functions, metabolic Identifying disorders and infections, as well as various physiological and pathological findings. 20 diagnostic methods commonly used to monitor conditions It is one of them. Proteins, pigments, metabolites, and cellular substances found in urine. The study of components, crystals, bacteria, and similar structures examines a person's health. It can provide important information regarding the condition. Therefore, a quick and reliable urine sample is essential. and analysis at regular intervals as much as possible, especially for clinical follow-up. It is important in this respect. 25 In current techniques, chemicals are commonly used for urine analysis. test strips, colorimetric methods, spectrophotometric measurement systems, and laboratory Automatic urine analyzers of this type are used. Reagent-based analyzers have been used for many years. On the test strips, the color changes by reacting with specific components in the urine. There are chemical regions that cause the change. The resulting color changes... Protein is evaluated visually or through automated reader systems. various parameters such as glucose, ketones, blood, bilirubin, urobilinogen, nitrite, leukocytes, and pH Information about it can be obtained. 2 Test strips are easy to use, but the measurement must be taken at a specific time. Because the analysis is done via a sample, the time-dependent changes in urine It is not suitable for continuous monitoring. A new measurement is required. A sample needs to be taken and a new test strip used. Also, the chemical... Reactive and disposable consumable materials are needed; sample collection, 5 during application and evaluation of the result by the user or healthcare personnel Intervention may be needed. This is especially true for long-term and frequent cases. This leads to increased workload and consumable consumption in patients requiring follow-up. It is possible. In order to evaluate the optical properties of urine in more detail, the laboratory Spectrophotometric systems of this type are also used. These systems utilize different wavelengths. While enabling the acquisition of detailed spectral data at various lengths, Generally, urine is collected in a separate sample container or cuvette, and then... It requires placement in the analysis device. Therefore, laboratory type 15 systems that allow urine to pass directly and continuously through a catheter or drainage line It is not suitable for monitoring purposes. Smaller optical sizes in the technical field. Although analytical systems also exist, a significant portion of them focus on a single analyte. determination, use of a limited number of wavelengths, or a specific optical It is directed towards the measurement mechanism. In some systems, determining the target component is possible. 20 Additionally, dyes, fluorescent markers, reagents, or sample pretreatment may be used. It may be necessary. One of the main problems in analyzing urine using optical methods is that the urine is very... It is a complex biological matrix containing numerous different components simultaneously. 25 Optical effects produced by hemoglobin, bilirubin, urobilinogen, and other components. They can overlap in certain spectral regions; cells, crystals, and bacteria. However, it can cause both light absorption and scattering. Urine Changes in color and turbidity can also affect the resulting optical signal. Therefore, the change occurring at a single wavelength is only relevant for a specific 30 Accepting that it originates from the analyte is a reliable result in all cases. It does not provide. 3 In addition, the optical properties of the components targeted for evaluation in urine are unique. It is not located in a spectral region. Some components are in the ultraviolet region, some are in the ultraviolet region. Significant optical changes in some cases in the visible region, and in others in the near-infrared region. It can cause multiple urine parameters to be detected in the same system. 5. Obtaining data from different spectral regions for evaluation. It is necessary. However, multi-wavelength measurements are required; different light the optical power of the sources, the spectral sensitivity of the photodetectors, and low This also makes it necessary to properly manage multi-level optical signals. Measurements taken at the catheter or drainage line will show a urine flow rate of 10 changes, air bubbles, cloudiness, and other issues that may occur on optical surfaces Contamination and changes in optical path geometry affect measurement results. This can affect the light source, sample, and therefore, in an inline analysis system. preserving the optical geometry between the photodetector and the low-level photodetector. reliable processing of signals and 15 obtained from different spectral channels It is important to evaluate the data together. Due to the aforementioned disadvantages observed in current systems, urine must be collected separately. catheter and / or reduces the need for sample collection and transportation to the laboratory. enabling the direct evaluation of raw urine passing through the drainage line, 20 Obtaining data from multiple spectral regions, reducing dependence on chemical reagents. capable of monitoring changes in urine continuously or at frequent intervals. An analysis system that provides this was needed. Developed in response to this need, the multi-wavelength inline urine analysis system and 25 The method of discovery utilizes different wavelengths in the ultraviolet, visible, and near-infrared regions. one or more LED light sources providing light along their lengths, measured spectral one or more photodetectors with appropriate sensitivity for the region, fixed optical path providing a sample and / or flow cell, a low-noise signal processing structure, and bringing together 30 multivariate data evaluation approaches within the same system The system allows measurements to be made in the spectral range of approximately 250–1800 nm. a single suitable light source that can emit light throughout this range or in selected portions of it broad-spectrum (full-spectrum) light-emitting diode (LED) or multiple LEDs It can be constructed in a modular structure that can include (for example, up to 30 LEDs). 4 Light emanates from the LED, passes through the urine in the sample container, and the sample... It is detected by a photodetector located on the opposite side of the tank. A single photodiode can be used as a photodetector, or photodiodes in different spectral regions can be used. In order to detect optical signals, each is appropriate to the spectral region being measured. Multiple photodiodes can also be used as selected. Thus, all 5 Information value in terms of targeted parameters rather than continuous measurement of the spectrum. Selective use of high wavelengths and different optics as needed. Channels can be added to or removed from the system. The system uses LEDs. It can be created in a rotational structure where it is arranged circularly, or in a small number of configurations. 10-inch wide-spectrum LEDs with a single photodetector, requiring no moving parts. It can also be produced in a smaller and simpler form. In this invention, optical data obtained from different wavelengths are independent of each other. instead of being evaluated as such, a multivariate evaluation approach should be used. is processed. In this way, the spectral 15 of different urine components overlap with each other. the differentiation of effects and interferences originating from the urine matrix The aim is to process protein, ascorbic acid, ketones, and hemoglobin through the system. A combination of bilirubin, urobilinogen, nitrite, and cellular or particulate turbidity. The aim is to evaluate multiple parameters. The invention is designed to be integrated into a catheter or drainage line. Thanks to this, urine can be collected optically through the line without the need to collect it in a separate container. examination of urine characteristics and changes that occur over time This allows for monitoring of changes. With a measurement structure that creates a fixed optical path... Increasing the repeatability of measurements, with low-noise signal processing. 25 Reliable processing of low-level signals obtained from photodetectors and with its modular light source structure, the system meets different analysis requirements. It becomes possible to adapt it. In conclusion, with the invention of the multi-wavelength inline urine analysis system and method, the existing 30 Batch measurement, reagent and consumable material needs encountered in urine analysis systems, Separate sampling and transportation, limited spectral evaluation, and single Reducing interference problems that can occur in wavelength-dependent measurements. The aim is to ensure that raw urine is processed within the pipeline and as much as possible. Evaluation without reactivity, multiple urine parameters being evaluated with the same optical system analysis through continuous or frequent monitoring and selected wave using a single photodetector with a small number of broad-spectrum LEDs or with different sizes Thanks to its modular structure, it is a more compact and lower-cost analysis system. It becomes possible to create it. 5 The structural and characteristic features and all the advantages of the invention are given in the figures below. And thanks to the detailed explanation provided, it will be understood more clearly, and therefore... This assessment is also made taking these figures and detailed explanations into consideration. It needs to be done. 10 Figures that Aid in Explaining the Invention The invention will be described by referring to the attached figures, so that the features of the invention can be explained. This will be understood more clearly. However, the purpose of this is to explain the invention with these specific arrangements. 15 It is not about limiting. On the contrary, the invention is defined by the accompanying claims. including all alternatives, modifications and equivalencies that can be included within the field That is also intended. The details shown are only the preferred aspect of the present invention. It is shown both for the purpose of explaining the regulations and the rules of the invention. to provide the most useful and easily understandable definition of its conceptual characteristics 20 It should be understood that they are presented for that purpose. Figure 1: The subject of the invention: a multi-wavelength inline urine analysis system and method. components of a sample arrangement of his invention featuring a rotational LED structure and Schematic view of the connections between the components 25 Reference Numbers That Aid in Disclosure of the Invention 1. LED light source 2. LED driver and power circuit board 30 3. Optical guidance and alignment module 4. Sample container 5. Optical filter 6. Optical sensing module 6 7. Analog-to-digital converter and microcontroller-based control unit 8. Multivariate calibration and analysis software. 9. User interface and data logging unit 10. Housing body Disclosure of the Invention This detailed explanation concerns the multi-wavelength inline urine analysis system and method. and solely for the purpose of better understanding the subject and without any limiting influence It is explained in a way that will not create a problem. The purpose of the invention is to provide a separate sample of urine passing through a catheter and / or drainage line. and by reducing the need for transfer to a laboratory environment, different wavelengths optical examination of their lengths and analysis of urine based on the optical data obtained enabling the evaluation of multiple parameters related to in-line use The goal is to develop a suitable, modular, and multi-wavelength urine analysis system. 15 Another aim of the invention is to explore ultraviolet, visible, and near-infrared spectral spectrums. obtained from measurements carried out at different wavelengths selected from the regions By evaluating the data together, different components in urine and Decomposition of optical effects originating from the urine matrix and multiple urine 20 The aim is to obtain information regarding the parameter. Another aim of the invention is to address the need for chemical reagents and disposable analytical consumables. by reducing the need for direct evaluation of raw urine, changes in urine 25 with a single or a small number of broad-spectrum LEDs and a single photodetector as needed The aim is to enable the creation of a compact analysis system that can be simplified. This invention was developed to achieve the aforementioned objectives; • approximately 30 covering the ultraviolet, visible and near-infrared spectral regions Providing optical radiation in the 250–1800 nm range, within that range a single LED or different wavelengths that emit light throughout or in selected sections targeted urine samples containing multiple LEDs that emit light along their length 7 Depending on the parameters, light-emitting diodes can be added, removed, or... Modular LED light source (1) which allows for replacement. • One or more light-emitting diodes in the LED light source (1) the light-emitting diodes in question together provide the necessary electrical power, LED 5 enables operation of the LEDs individually or according to a specified measurement sequence. driver and power circuit board (2), • LED light located between the LED light source (1) and the sample container (4) directing the light emitted from the source (1) into the sample chamber (4) and in the measurement The sample chamber (4) and optical detection module (6) of the LED used are the same. Optical orientation and alignment that ensures it remains on the optical axis 10 module (3), • A container holding the urine sample to be analyzed, illuminated by light. a quartz crystal that allows the sample to pass along a defined optical path. can be configured as a basic sample cuvette or in-line flow cell sample container (4), 15 • located between the sample container (4) and the optical detection module (6), Optical radiation passing through the sample chamber (4) will be used for measurement Selecting spectral components and measuring unwanted spectral components. optical filter (5) that reduces its effect on it, • 20 on the opposite side of the sample container (4) relative to the LED light source (1). positioned, emitting light from the LED light source (1) into the sample container (4) by detecting light passing through the urine and, if necessary, light scattered by the sample a single photodiode or a measured spectral range that converts into an electrical signal Optical sensing module containing multiple photodiodes selected accordingly (6), • The operating sequence of the light-emitting diodes in the LED light source (1) and 25 obtained from the optical sensing module (6) which controls the measurement processes converting analog signals into digital data and the digital data in question an analog-to-digital converter that enables the data to be transferred for processing and microcontroller-based control unit (7), • Processes optical data obtained from different wavelengths, dark signal and 30 Correcting measurement data according to reference measurements, transmission and absorbance. calculating values ​​and using data obtained from different spectral channels multivariate calibration, which allows for variable evaluation. analysis software (8), 8 • presents measurement data and analysis results to the user, and provides the data in question. visualization and data obtained as a result of measurement and analysis User interface and data logging unit (9) that enables recording, • housing the optical and electronic components of the system in designated locations protecting the components in question from external environmental influences, 5% of ambient light limiting access to the optical detection module (6) and optical measurement Housing body (10) which ensures the preservation of its geometry, • approximately 250– in the ultraviolet, visible and near-infrared spectral regions producing light at different wavelengths or in a broad spectrum in the 1800 nm range. and enables the determination of different spectral properties of urine samples, 10 a single option selectable according to the urine parameters targeted by the user can be configured as a single broad-spectrum LED or multiple LEDs. light-emitting diodes (LEDs), • In arrangements where the LED light source (1) contains more than one LED, the said 15 LEDs arranged in a circular pattern, emitting light of different wavelengths. Activating diodes sequentially, optically directing and aligning the selected LED. through module (3) sample chamber (4) and optical detection module (6) repeatable measurements at different wavelengths by bringing them to the same optical axis. rotational optical alignment mechanism that enables this, • located in the optical sensing module (6), passing through the sample chamber (4) and 20 detecting light scattered by the sample, converting the optical radiation into an electrical signal. converting, sensitive to ultraviolet, visible and near-infrared spectral regions. either a single photodiode or each photodiode corresponding to the measured spectral region. Photodetectors consisting of multiple photodiodes selected as such, • Low-level electrical signals obtained from photodetectors can be measured. 25 raising the level of unwanted electrical noise in those signals an analog signal that reduces and transmits signals to the control unit (7) amplification and filtering circuit, • Integrated into the catheter and / or drainage line, measuring urine from an optical measurement area and 30 in-line without the need to collect urine in a separate sample container. flow cell that enables measurement, • Collection and measurement of urine from a catheter or drainage line into a flow cell. Urine inlet that allows urine to pass through the region and be removed from the flow cell. and output connections, 9 • a light intensity determined from a urine sample and kept constant between measurements enabling passage over a distance, thereby facilitating optical geometry and measurement. a fixed optical path that ensures the preservation of repeatability • Fundamental optics against which optical measurements obtained from the sample will be compared 5 that determine the values ​​and consist of light sources and sensing elements eliminating the effect of resulting changes on the measurement results reference measurement structure, • Measurement signals obtained from photodetectors are converted into dark signal and reference signals. Corrected according to measurement values ​​and measurements obtained from different wavelengths. The signal processing architecture that enables the data to be analyzed, 10 • Reference measurement values ​​and measurement values ​​obtained from the sample comparing and determining the light transmittance of urine samples at different wavelengths. The transmission calculation structure that enables its determination, • Different waveforms of urine samples using reference and transmission data Absorbance, which enables the determination of light absorption behavior at different lengths. computational structure, • air bubbles, turbidity, and excessive noise that may occur during inline measurement scattering, optical surface contamination and similar conditions affecting measurement a measurement that determines and enables the distinction between reliable and unreliable measurements quality control structure, 20 • Multivariate numerical measurement data obtained from the control unit (7) calibration and analysis software (8), and the data obtained as a result of the analysis Data communication structure that transmits to the user interface and data logging unit (9) It includes. In the system described in the invention, light is emitted from an LED light source (1) and is optically directed and The sample reaches the sample chamber (4) via the alignment module (3), sample After passing through the urine in the reservoir (4), the sample passes through the optical filter (5). The signal is transmitted to the optical sensing module (6) located on the opposite side of the reservoir (4). Thus, the sequence LED – sample chamber (cuvette or flow cell) – detector is 30. A transmission measurement geometry is being created. LED light source (1) provides light throughout the approximately 250–1800 nm range or selected with a single broad-spectrum (full-spectrum) LED that can emit light in its sections They can be created by using light of different wavelengths or different spectral bands. It can also be created with multiple emitting LEDs. Similarly, optical sensing. The module (6) may contain a single photodiode or, depending on the measured spectral range It can also include multiple selected photodiodes. For example, up to approximately 1100 nm. For the ultraviolet, visible and near-infrared regions, a silicon (Si) based, longer 5 For the near-infrared region, indium gallium arsenide (InGaAs) based materials are used. Photodiodes can be used, and the relevant spectral value can be obtained in the measurement at each wavelength. The signal from a photodiode suitable for the region can be evaluated. As shown in Figure 1, the LEDs are arranged in a circular pattern and 10 LEDs will be used for measurement. The rotational configuration, in which the LED is rotated to align with the optical axis, is the only invention of its kind. It is a sample arrangement. In another arrangement of the invention, a single broad Spectrum LEDs or a small number of LEDs with a single photodiode are used; LED light source (1), sample chamber (4) and optical sensing module (6) are movable parts. without requiring, they are positioned sequentially on a fixed optical axis, optical 15 guidance and alignment module (3) in the form of a fixed lens and / or holder structure Spectral channels to be created and used in measurement will be one or more optical channels. It is separated through the filter (5). This arrangement makes the system smaller, more This allows it to be produced as a simpler and lower-cost product. Multi-wavelength inline urine analysis system and method; • When the system is started, the LED light source (1) is in the off position. the dark signal values ​​are recorded via the optical detection module (6) obtaining, • taking the reference liquid into the sample container (4) and turning on the LED light source (1) 25 Enabling via LED driver and power circuit board (2), multiple If LEDs are present, LEDs of different wavelengths can be arranged in sequence. activation, • The optical sensing module (6) detects the light passing through the reference liquid. perception and reference light intensity values ​​for each wavelength 30 analog-to-digital converter and microcontroller-based control unit (7) transfer, 11 • taking the urine sample to be analyzed into the sample container (4) or urine from the catheter or drainage line passes through an in-line flow cell passing through the sample container (4), • LED light source (1) through LED driver and power circuit board (2) Activation means that if there are multiple LEDs, the designated 5 LEDs will be activated. Activation of each section separately according to the measurement sequence, • Optical guidance and alignment module of the light emitted from the LED light source (1) (3) through the same optical sample chamber (4) and optical detection module (6) rotational alignment where LEDs are placed circularly on the axis In this arrangement, the LED to be used for measurement is rotated and aligned with the optical 10 in question. alignment with the axis, • The light emitted from the LED light source (1) is then placed in the sample chamber (4) from the urine sample found along the fixed optical path and through the optical filter (5) and the passage of light passing through or scattered by the sample Optical sensing module (6) located on the opposite side of the sample chamber (4) 15 perception by • If the optical sensing module (6) contains more than one photodiode, each In wavelength measurement, the signal of the photodiode suitable for the relevant spectral region if a single broad-spectrum LED is used, then in the measurement optical filter (5) and / or different spectral sensitivity of spectral channels to be used 20 separation via photodiodes, • low-level electrical signals obtained in the optical sensing module (6) amplification of the analog signal through an amplification and filtering circuit and reducing electrical noise, • amplified analog measurement signals are converted to analog-to-digital converter and 25 through the microcontroller-based control unit (7) to digital data transformation, • Sample measurement values ​​obtained for each wavelength, dark signal and multivariate calibration and analysis using reference measurement values. (8) correction in the software, 30 12 • For each wavelength, using corrected optical measurement data Multivariate calibration and analysis of transmission and absorbance values Calculation through software (8), • absorbance, transmission, and scattering obtained from different wavelengths By combining the related optical data, a multi-channel optical analysis of the urine sample is performed. creation of the data sequence, • Multivariate calibration and analysis of the generated multi-channel optical data array together with the software (8) and the different in urine spectral variations resulting from components, turbidity, and matrix effects. Separation of initiatives, 10 • as a result of evaluating multi-channel optical data with a calibration model Quantitative, semi-quantitative, positive-negative or Generating analysis results that include a level of confidence, • Air bubbles, excessive turbidity, flow variation, optical issues during inline measurement. Surface contamination or similar conditions affecting measurement may cause different optical 15 determination from inconsistent signal changes occurring in the channels and separating unreliable measurements, • User interface and data of the obtained measurement data and analysis results transfer to the registration unit (9), display to the user and record to be taken, 20 • In applications where inline measurement continues, urine from the sample container (4) the transmission continues and multiple waves are carried out at specified time intervals. The process involves repeating the longitudinal optical measurement procedure. All technical and other specifications mentioned in each request are followed by a reference number. 25 This reference number is provided solely to facilitate understanding of the requests. They have been used, therefore this reference number is for illustrative purposes only. It should not be assumed that the scope of the element indicated is limited. A technically skilled person can demonstrate the novelty presented in the invention, and similar structures can be developed in 30 years. by using and / or demonstrating this structure in the relevant technique 13 It is clear that this can be applied to other fields with similar purposes. Therefore, such a It is also obvious that these structures will lack the criterion of surpassing innovation. 10 20 30

Claims

14 REQUESTS 1. The invention relates to a multi-wavelength inline urine analysis system, characterized by: • approximately 5 covering the ultraviolet, visible and near-infrared spectral regions. Providing optical radiation in the 250–1800 nm range, within that range a single LED or different wavelengths that emit light throughout or in selected sections targeted urine samples containing multiple LEDs that emit light along their length Depending on the parameters, light-emitting diodes can be added, removed, or... Modular LED light source (1) which allows for replacement, 10 • One or more light-emitting diodes in the LED light source (1) the light-emitting diodes in question together provide the necessary electrical power, LEDs that allow operation individually or according to a specified measurement sequence. driver and power circuit board (2), • Located between the LED light source (1) and the sample container (4), the LED light 15 directing the light emitted from the source (1) into the sample chamber (4) and in the measurement The sample chamber (4) and optical detection module (6) of the LED used are the same. optical orientation and alignment that ensures it remains on the optical axis module (3), • A container holding the urine sample to be analyzed, illuminated by light, contains urine 20 a quartz crystal that allows the sample to pass along a defined optical path. can be configured as a basic sample cuvette or in-line flow cell sample container (4), • located between the sample container (4) and the optical detection module (6), 25 to be used in measurement through optical radiation passing through the sample chamber (4) Selecting spectral components and measuring unwanted spectral components. optical filter (5) that reduces its effect on it, • on the opposite side of the sample container (4) relative to the LED light source (1) positioned, emitting light from the LED light source (1) into the sample container (4) By detecting light passing through the urine and, if necessary, scattered by the sample, 30 a single photodiode or a measured spectral range that converts into an electrical signal Optical sensing module containing multiple photodiodes selected accordingly (6), • The operating sequence of the light-emitting diodes in the LED light source (1) and obtained from the optical sensing module (6) which controls the measurement processes converting analog signals into digital data and the digital data in question an analog-to-digital converter that enables the data to be transferred for processing and microcontroller-based control unit (7), • Processes optical data obtained from different wavelengths, dark signal and Correcting measurement data according to reference measurements, transmission and absorbance 5 calculating values ​​and using data obtained from different spectral channels multivariate calibration, which allows for variable evaluation. analysis software (8), • presents measurement data and analysis results to the user, and provides the data in question. the visualization and the data obtained as a result of measurement and analysis 10 User interface and data logging unit (9) that enables recording, • housing the optical and electronic components of the system in designated locations protecting the components in question from external environmental influences, ambient light limiting access to the optical detection module (6) and optical measurement parts of the housing body (10) which ensure the preservation of its geometry 15 It includes.

2. This relates to a multi-wavelength inline urine analysis system in accordance with Claim 1, feature; • approximately 250– in the ultraviolet, visible and near-infrared spectral regions 20 devices that produce light at different wavelengths or in a broad spectrum in the 1800 nm range. and enables the determination of different spectral characteristics of urine samples, a single option selectable according to the urine parameters targeted by the user can be configured as a single broad-spectrum LED or multiple LEDs. light-emitting diodes (LEDs), • In arrangements where the LED light source (1) contains more than one LED, the aforementioned 25 LEDs arranged in a circular pattern, emitting light of different wavelengths. Activating diodes sequentially, optically directing and aligning the selected LED. through module (3) sample chamber (4) and optical detection module (6) repeatable measurements at different wavelengths by bringing them to the same optical axis. The rotational optical alignment mechanism that enables this is 30 • located in the optical sensing module (6), passing through the sample chamber (4) and detecting light scattered by the sample, converting the optical radiation into an electrical signal. converting, sensitive to ultraviolet, visible and near-infrared spectral regions. 16 either a single photodiode or each photodiode corresponding to the measured spectral region. Photodetectors consisting of multiple photodiodes selected as such, • Low-level electrical signals obtained from photodetectors can be measured. raising the level of unwanted electrical noise in those signals analog signal 5 which reduces and enables the signals to be transmitted to the control unit (7) amplification and filtering circuit, • Integrated into the catheter and / or drainage line, measuring urine from an optical measurement area and allows urine to pass through in the line without the need to collect urine in a separate sample container. flow cell that enables measurement, • Collection of urine from the catheter or drainage line into the flow cell and measurement 10 Urine inlet that allows urine to pass through the region and be removed from the flow cell. and output connections, • a light intensity determined from a urine sample and kept constant between measurements enabling passage over a distance, thereby facilitating optical geometry and measurement. a fixed optical path that ensures repeatability is maintained, 15 • Fundamental optics against which optical measurements obtained from the sample will be compared values ​​determined by light sources and sensing elements eliminating the effect of resulting changes on the measurement results reference measurement structure, • Measurement signals obtained from photodetectors are compared with dark signal and reference 20 Corrected according to measurement values ​​and measurements obtained from different wavelengths. A signal processing architecture that enables data to be made analyzable. • Reference measurement values ​​and measurement values ​​obtained from the sample comparing and determining the light transmittance of urine samples at different wavelengths. The transmission calculation structure that enables its determination, 25 • Different waveforms of urine samples using reference and transmission data Absorbance, which allows us to determine the light absorption behavior of different lengths. computational structure, • air bubbles, turbidity, and excessive noise that may occur during inline measurement scattering, optical surface contamination and similar conditions affecting measurement 30 a measurement that determines and enables the distinction between reliable and unreliable measurements quality control structure, • Multivariate numerical measurement data obtained from the control unit (7) calibration and analysis software (8), and the data obtained as a result of the analysis 17 parts of the data communication structure that transmit to the user interface and data logging unit (9) It includes.

3. This relates to a multi-wavelength inline urine analysis system in accordance with Claim 1, Its feature is that the optical sensing module (6) has 5 different spectral regions, each of which is different. It contains multiple photodiodes selected to be sensitive, and each wave in the measurement at the neck, the spectral region where the wavelength in question is located The key is using a suitable photodiode.

4. This relates to a multi-wavelength inline urine analysis system in accordance with Claim 1, 10 Its feature is that the LED light source (1) emits light in the range of approximately 250–1800 nm. Containing a single broad-spectrum LED or a small number of LEDs, optical sensing. The module (6) contains a single photodiode and the LED light source (1), sample a movable alignment element of the reservoir (4) and the optical sensing module (6) It is the sequential positioning of objects along a fixed optical axis without requiring any prior adjustment. 15 5. The invention relates to a multi-wavelength inline urine analysis system and method, feature; • When the system is started, the LED light source (1) is in the off position. the dark signal values ​​are 20 through the optical detection module (6) obtaining, • taking the reference liquid into the sample container (4) and turning on the LED light source (1) Enabling via LED driver and power circuit board (2), multiple If LEDs are present, LEDs of different wavelengths can be arranged in sequence. activation, 25 • The optical sensing module (6) detects the light passing through the reference liquid. perception and reference light intensity values ​​for each wavelength analog-to-digital converter and microcontroller-based control unit (7) transfer, • taking the urine sample to be analyzed into the sample container (4) or 30 urine from the catheter or drainage line passes through an in-line flow cell passing through the sample container (4), 18 • LED light source (1) through LED driver and power circuit board (2) If multiple LEDs are present, their activation will determine the direction of the LEDs. Activation of each section separately according to the measurement sequence, • Optical guidance and alignment module of the light emitted from the LED light source (1) (3) through the same optical 5 sample chamber (4) and optical detection module (6) rotational alignment where LEDs are placed circularly on the axis In this arrangement, the LED to be used for measurement is rotated to create the optical display. alignment with the axis, • The light emitted from the LED light source (1) is then placed in the sample chamber (4) from the urine sample found along the fixed optical path and through the optical filter (5) 10 and the passage of light passing through or scattered by the sample Optical sensing module (6) located on the opposite side of the sample chamber (4) perception by • If the optical sensing module (6) contains more than one photodiode, each In the measurement at the wavelength, the signal of the photodiode suitable for the relevant spectral region is 15. if a single broad-spectrum LED is used, then in the measurement optical filter (5) and / or different spectral sensitivity of spectral channels to be used separation via photodiodes, • low-level electrical signals obtained in the optical sensing module (6) amplification of the analog signal through an amplification and filtering circuit and 20 reducing electrical noise, • analog-to-digital converter of amplified analog measurement signals and through the microcontroller-based control unit (7) to digital data transformation, • Sample measurement values ​​obtained for each wavelength, dark signal 25 and multivariate calibration and analysis using reference measurement values. (8) correction in the software, • For each wavelength, using corrected optical measurement data Multivariate calibration and analysis of transmission and absorbance values Calculation via software (8), 30 • absorbance, transmission, and scattering obtained from different wavelengths Multichannel optical data of the urine sample are collected by combining the relevant optical data. creation of the data sequence, 19 • Multivariate calibration and analysis of the generated multi-channel optical data array together with the software (8) and the different in urine spectral variations resulting from components, turbidity, and matrix effects. separating the initiatives, • As a result of evaluating multi-channel optical data with a calibration model 5 Quantitative, semi-quantitative, positive-negative or Generating analysis results that include a level of confidence, • Air bubbles, excessive turbidity, flow variation, optical issues during inline measurement. Surface contamination or similar conditions affecting measurement can cause different optics. Identification from inconsistent signal changes occurring in the channels and 10 separating unreliable measurements, • User interface and data of the obtained measurement data and analysis results transfer to the registration unit (9), display to the user and record taking, • In applications where inline measurement continues, urine from the sample container (4) 15 the transmission continues and multiple waves are carried out at specified time intervals. The process involves repeating the longitudinal optical measurement procedure, including the procedural steps. 25