Microfluidic cartridge

Abstract

A microfluidic cartridge, comprising a test cartridge (100) and an insertion member (180), the insertion member (180) being used for being inserted into the test cartridge (100). The test cartridge(100) comprises a base (120) and an upper cover (160), and the insertion member (180) comprises a plate-shaped member (182) and a plurality of tubular objects (184) used for being inserted into the test cartridge (100). The base (120) comprises a receiving tank (122), a plurality of reaction tanks (124), a plurality of flow guide channels (126), and a plurality of indication recesses (128) provided on the base (120). The upper cover (160) covers the base (120), and the upper cover (160) is provided with an injection hole (162), a plurality of tests holes respectively arranged corresponding to the plurality of reaction tanks (124), and a flow guide member (166). The insertion member (180) comprises the plate-shaped member (182) and the plurality of tubular objects (184) provided on the plate-shaped member (182). When the insertion member (180) is inserted into the test cartridge (100), the plate-shaped member (182) is located above the upper cover (160), and the tubular objects (184) of the insertion member (180) are respectively inserted into the reaction tanks (124) of the base (120) through reaction holes of the upper cover (160), such that a sample fluid is retained in the tubular objects (184).

Description

Microfluidic Cassette Technical Field

[0001] The present invention relates to a microfluidic cartridge, and more particularly to a microfluidic cartridge capable of completing a detection reaction without requiring external power. Background Art

[0002] Micro-test reagents offer numerous advantages, enabling the completion of various clinical tests using low sample volumes. These reagents are developed to be compatible with a variety of immunoassay methods (e.g., direct detection, sandwich assays, or competitive assays). For medical testing, these reagents offer advantages such as convenience, speed, and safety.

[0003] However, in the detection of the microfluidic channel of the meristem test, external power is required, using a micro pump to drive the flow of the sample fluid through the pressure difference, and using a micro valve to control the flow direction of the sample fluid in the test microchannel. Therefore, it can be seen that the microfluidic cartridge of the prior art has some limitations in use.

[0004] In view of this, in order to improve the defects of the prior art, there is an urgent need in the art for an improved test reagent to improve the deficiencies of the prior art.

[0005] Summary of the Invention

[0006] The invention summary is intended to provide a simplified summary of the content of this application so that readers can have a basic understanding of the content of this application. This invention summary is not a complete overview of the content of this application and is not intended to identify important / critical elements of the embodiments of the present invention or to define the scope of the present invention.

[0007] To address the deficiencies of the prior art, one aspect of the present invention relates to a novel microfluidic cartridge that can complete testing steps without the need for external power. Specifically, the microfluidic cartridge comprises a test cartridge and an insert. The test cartridge comprises a base and a top cover, wherein the base is provided with a receiving tank, a plurality of reaction tanks, a plurality of drainage channels, a plurality of indicator tanks, and a collection tank. The receiving tank comprises a plurality of buffer flow channels, the plurality of reaction tanks are located downstream of the receiving tank, and each drainage channel is in fluid communication with the receiving tank at one end and in fluid communication with the reaction tanks at the other end for guiding sample fluid from the receiving tank to the reaction tanks. The indicator tanks are in fluid communication with the reaction tanks for receiving sample fluid flowing out of the reaction tanks, and the collection tank is in fluid communication with the indicator tank for receiving sample fluid flowing out of the indicator tanks. The upper cover is disposed on the base and is structurally provided with an injection hole, a plurality of inspection holes, and a flow guide. The injection hole is disposed corresponding to the receiving slot on the base, and the inspection holes are disposed corresponding to the reaction slots. The flow guide is located below the upper cover and corresponds to the buffer flow channels or the drainage channels of the receiving slot, and is in fluid communication with the buffer flow channels or the drainage channels, for guiding the sample fluid from the receiving slot to the reaction slot. The plug-in is configured to be inserted into the plurality of reaction slots of the microfluidic cartridge and structurally comprises a plate-like member and a plurality of tubes, wherein the tubes are arranged in an array on the plate-like member and are disposed corresponding to the reaction slots. The tubes are configured to penetrate the inspection holes of the upper cover and be inserted into the reaction slots of the base, respectively, for causing the sample fluid in the reaction slots to be retained within the tubes.

[0008] In addition, in order to prevent the sample fluid from flowing back, a first tilt angle is configured at the connection point between the reaction tanks and the indicator tank fluids in the microchannel cartridge, and a second tilt angle is configured at the connection point between the sample fluids in the indicator tanks and the collection tank to prevent the sample fluid from flowing back into the indicator tank.

[0009] According to a specific embodiment of the present invention, the microfluidic cartridge of the present invention has three reaction slots and three indicator slots respectively arranged in a row, and the fluid connection between the reaction slot located in the middle of the reaction slots and the corresponding indicator slot is fan-shaped.

[0010] In one embodiment of the present invention, the microfluidic cartridge further comprises a sample cover for fitting over the injection port. In other embodiments, the microfluidic cartridge further comprises a reaction tank cover for detachably fitting over the tubular members of the insert.

[0011] According to one embodiment of the present invention, the microfluidic cartridge further includes a viewing window disposed corresponding to the indicator slot.

[0012] According to one embodiment of the present invention, the microfluidic cartridge further includes a plurality of anti-overflow rings respectively disposed on the upper edges of the plurality of inspection holes.

[0013] According to a specific embodiment of the present invention, the bottom of the base of the microfluidic cartridge corresponding to the plurality of reaction tanks is more protruding than the bottom around the base.

[0014] Another embodiment of the present invention is directed to a method for performing an inspection using the microfluidic cartridge described in any of the above embodiments, the method comprising the following steps:

[0015] (a) injecting a sample fluid into the base through the injection hole of the upper cover, wherein the sample fluid flows through the receiving tank and the drainage channels into the reaction tank, the indicator tank, and the collection tank respectively;

[0016] (b) inserting the insert through the upper cover into the base, wherein the tubes of the insert are respectively inserted into the reaction chambers of the base, so that the sample fluid is retained in the tubes; and

[0017] (c) detecting the content of a test substance in the sample fluid in the tubes.

[0018] In a specific embodiment, the step (c) is to detect the fluorescence value of the sample fluid in the tubes through a fluorescence detection device.

[0019] After reading the following embodiments, a person having ordinary knowledge in the technical field to which the present invention belongs will be able to easily understand the basic spirit and other invention objectives of the present invention, as well as the technical means and implementation aspects adopted by the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] To make the above and other objects, features, advantages and embodiments of the present invention more clearly understood, the accompanying drawings are described as follows:

[0021] FIG1A and FIG1B are schematic diagrams of a microfluidic cartridge 1000 according to one embodiment of the present invention;

[0022] FIG2A is a top view of the base 120 of the microfluidic cartridge shown in FIG1A ;

[0023] FIG2B is a cross-sectional view of the base 120 taken along line 2A-2A of FIG2A;

[0024] FIG2C is a bottom view of the base 120 of the microfluidic cartridge shown in FIG2A ;

[0025] FIG3A is a top view of the upper cover 160 of the microfluidic cassette shown in FIG1A ;

[0026] FIG3B is a bottom view of the upper cover 160 of the microfluidic cartridge;

[0027] FIG3C is a cross-sectional view of the upper cover 160 taken along line 3C-3C of FIG3B ;

[0028] FIG. 4A and FIG. 4B are schematic diagrams of the structure of the plug-in 180 shown in FIG. 1A ;

[0029] FIG5 is a cross-sectional view of the microfluidic cartridge 1000 shown in FIG1A;

[0030] 6A to 6C are schematic structural diagrams of a microfluidic cartridge 7000 according to another embodiment of the present invention; and

[0031] 7A to 7E are schematic diagrams illustrating an inspection method performed using the microfluidic cartridge 1000 shown in FIG. 1A and FIG. 1B .

[0032] According to conventional practice, the various features and components in the drawings are not drawn to scale. The drawing method is to best present the specific features and components related to the present invention. In addition, the same or similar reference numerals are used to refer to similar components / parts in different drawings. DETAILED DESCRIPTION

[0033] To provide a more detailed and complete description of the present disclosure, the following provides illustrative descriptions of various embodiments and examples of the present disclosure. However, these descriptions are not intended to be the only ways to implement or use the embodiments of the present disclosure. The detailed descriptions cover features of various embodiments and the method steps and sequences for constructing and operating these embodiments. However, other embodiments may also be used to achieve the same or equivalent functionality and step sequences.

[0034] Although the numerical ranges and parameters used to define the broader scope of the present invention are approximate, the numerical values ​​of the specific examples are presented herein as precisely as possible. However, any numerical value inherently contains standard deviations that are inherent to the testing methods used. As used herein, "about" generally means that the actual value is within plus or minus 10%, 5%, 1%, or 0.5% of a particular value or range. Alternatively, the term "about" means that the actual value falls within an acceptable standard error of the mean, as determined by one of ordinary skill in the art. Except in the experimental examples, or unless otherwise expressly indicated, all ranges, amounts, values, and percentages used herein (e.g., to describe material amounts, time periods, temperatures, operating conditions, quantitative ratios, and the like) are to be understood as modified by the word "about." Therefore, unless otherwise indicated, the numerical parameters disclosed in this specification and the accompanying claims are approximate and are subject to change as needed. At a minimum, these numerical parameters should be understood to include the number of significant digits indicated and to apply normal rounding.

[0035] The term "sample fluid" herein refers to a sample suitable for use with the microfluidic cartridge 100 of the present invention, preferably a biological sample. In one optional embodiment, the biological sample may be blood, body fluids, saliva, or other secretions. In another optional embodiment, the biological sample may be a processed biological sample, for example, a biological sample pre-treated with a buffer.

[0036] Unless otherwise defined herein, scientific and technical terms used herein have the same meanings as those commonly understood and used by persons skilled in the art in the art to which this invention belongs. Furthermore, unless otherwise defined in the context, singular nouns used in this specification include the plural form of such nouns, and plural nouns also include the singular form of such nouns.

[0037] The present invention proposes a novel microfluidic cartridge that, through the design of its flow channels and components, can complete detection reactions without the need for external force assistance (e.g., gas pressurization). Furthermore, the microfluidic cartridge of the present invention is equipped with multiple reaction tanks, allowing for simultaneous testing of different test items on a single sample, or for simultaneous testing of multiple samples.

[0038] 1A and 1B are schematic diagrams of a microfluidic cassette 1000 according to one embodiment of the present invention, wherein FIG1A is an exploded view of the microfluidic cassette 1000. The microfluidic cassette 1000 of the present invention mainly comprises a test cassette 100 and an insert 180.

[0039] As shown in FIG1A , the test cassette 100 includes a base 120 and a cover 160 , which are coupled vertically to form the test cassette 100. Those skilled in the art will appreciate that the coupling structure between the cover 160 and the base 120 can be achieved using existing snap-fit ​​structures or other coupling methods. Referring also to FIG1B , the insert 180 is removably inserted into the base 120 and cover 160 of the test cassette 100.

[0040] To clearly understand the structure of the base 120 of the present test cassette 100, please refer to Figures 2A to 2C simultaneously, wherein Figure 2A is a top view of the base 120 of the test cassette shown in Figure 1; Figure 2B is a cross-sectional view of the base 120 drawn along line 2B-2B of Figure 2A; and Figure 2C is a bottom view of the base 120 of the microfluidic cassette.

[0041] As shown in FIG. 2A , the base 120 includes a receiving reservoir 122, a plurality of reaction reservoirs 124, a plurality of drainage channels 126, a plurality of indicator reservoirs 128, and a collection reservoir 130, all disposed on the base 120 and in fluid communication with one another. In one embodiment, the reservoirs of the base 120 are arranged, from upstream to downstream, as the receiving reservoir 122, the reaction reservoir 124, the indicator reservoir 128, and the collection reservoir 130. Sample fluid (e.g., a test sample) enters the reaction reservoir 124 from the receiving reservoir 122, and excess sample fluid overflows into the indicator reservoir 128 and the collection reservoir 130. In a preferred embodiment, in the base 120 of the microfluidic cartridge of the present invention, the sample fluid flows in a unidirectional manner through the receiving reservoir 122, the reaction reservoir 124, and the indicator reservoir 128, i.e., the sample fluid does not flow back.

[0042] In this embodiment, the receiving tank 122 is provided with four radially arranged buffer channels 123. One end of each of the four buffer channels 123 is adjacent to and connected to three drainage channels 126. Note that the buffer channels 123 are droplet-shaped, with the ends not connected to the drainage channels 126 being wider than the ends connected to the drainage channels 126. These drainage channels 126 then connect to the three reaction tanks 124, respectively, to guide sample fluid from the receiving tank 122 to each of the reaction tanks 124 to facilitate reaction. In this embodiment, three indicator tanks 128 are provided, each in fluid communication with the reaction tanks 124. Sample fluid overflowing from the reaction tanks 124 flows into the indicator tanks 128 and then into the collection tank 130. In addition, the buffer channel 123 on the receiving tank 122 enters the drainage channel 126 . The inclination of the buffer channel 123 is at least about 1 degree, preferably 1.5 degrees, to help the sample fluid flow into the drainage channel 126 and prevent it from flowing back into the buffer channel 123 .

[0043] To prevent sample fluid backflow, an angled design can be provided between the various slots in the microfluidic cartridge. Referring to Figures 2B and 2C , the fluid connections between the reaction slots 124 and the indicator slots 128 are configured with a first angled design A1, and the connection between the sample fluid in the indicator slots 128 and the collection slot 130 is configured with a second angled design A2 to prevent sample fluid backflow into the indicator slots. The first angled design is tilted from the reaction slots 124 toward the indicator slots 128, with the plane corresponding to the fluid connection with the reaction slots 124 being higher than the plane of the indicator slots 128. The second angled design is tilted from the indicator slots 128 toward the collection slot 130, with the plane proximate the indicator slots 128 being higher than the plane proximate the collection slots 130. The first and second angles are at least 10 degrees, for example, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, and 20, and preferably at least 15 degrees.

[0044] Furthermore, please refer to Figure 2A again. This embodiment has three reaction tanks 124, among which the fluid connection between the middle reaction tank 124M and the corresponding indicator tank 128 is fan-shaped. This design is to allow the sample fluid to flow to the indicator tank 128 and the recovery tank 130 at a constant speed.

[0045] In addition, please refer to Figure 2C, which is a bottom view of the base 120. According to one embodiment of the present invention, the portion of the base 120 corresponding to the reaction tank 124 is protruded from the bottom of the base 120 relative to the surrounding area. As shown in the figure, the portion corresponding to the reaction tank 124 protrudes from the bottom of the base 120 to facilitate heating of the reaction tank 124. Furthermore, to increase the volume of the collection tank 130, the portion of the bottom corresponding to the collection tank 130 may also protrude from the bottom of the base 120.

[0046] FIG3A is a top view of the upper cover 160 shown in FIG1 , and FIG3B is a bottom view of the upper cover 160 . Specifically, the upper cover 160 is coupled to the base 120 to form the test cassette 100 , and is preferably connected using a snap-fit ​​structure. The upper cover 160 is structurally provided with an injection hole 162 , a plurality of test holes 164 , and a flow guide 166 . The injection hole 162 is provided corresponding to the receiving slot 122 on the base 120 , and the plurality of test holes 164 are provided corresponding to the reaction slots 124 . The flow guide 166 is provided corresponding to the buffer flow channels 123 and / or the drainage channels 126 of the receiving slot 122 and is fluidically connected thereto, for guiding the sample fluid from the receiving slot 122 to the reaction slot 124 .

[0047] FIG3C is a cross-sectional view of the upper cover 160, clearly illustrating the structure of the flow guide 166. Referring to FIG3C and FIG2A together, the flow guide 166 is structurally composed of at least two flow guide plates, namely, flow guide plates 167A and 167B. The outer sides of flow guide plates 167A and 167B are provided with an inclined angle, and each of flow guide plates 167A and 167B defines a flow channel 168 at a distance therebetween, thereby establishing fluid communication between the flow guide 166 and the buffer flow channels 123, the drainage channels 126, and the reaction tanks 124 of the receiving tank 122.

[0048] Furthermore, three viewing windows 104 may be provided on the upper cover 160, corresponding to the three indicator slots 128, respectively. Through the viewing windows 104, it is possible to inspect whether the sample fluid has flowed into the indicator slots 128, thereby confirming whether the sample volume is sufficient. In other embodiments, the plurality of viewing windows 104 may be a single viewing window, with the opening of the single viewing window being positioned corresponding to the indicator slots 128, for observing whether the sample fluid has flowed into the indicator slots.

[0049] Referring again to FIG. 3A , the injection hole 162 is specially designed. A slit 163 is provided at the injection hole 162 , and below the slit 163 is a portion of a flow guide 166 , which corresponds to the configuration of the buffer flow channel 123 . The slit structure of the slit 163 ensures that, after the sample is injected into the injection hole 162 , the sample fluid is retained in the injection hole 162 and then guided into the reaction tank 124 through the flow guide 166 .

[0050] In this embodiment, the base 120 of the microfluidic cartridge 100 of the present invention is provided with three reaction tanks 124. To control the flow rate and flow velocity of the sample fluid entering each reaction tank 124, the widths of the flow channels 168a, 168b, and 168c corresponding to these drainage channels 126 can be adjusted. For example, the widths of the two outer channels 168a and 168c are greater than the width of 168b, so that the speed of the sample fluid flowing from the receiving tank 122 to each reaction tank 124 is approximately the same.

[0051] According to another embodiment of the present invention, the microfluidic cartridge 100 of the present invention further includes a plurality of anti-overflow rings 170 respectively disposed on the upper edges of the plurality of inspection holes 164 .

[0052] It can be seen from this that the microfluidic cartridge 100 proposed in the present invention allows the sample fluid to enter the buffer channel 123 through the injection port 162 and the guide member 166 through the drainage channel 126 to the reaction tank 124 through the drainage channel 126. Excess sample overflows into the indicator tank 128 and then enters the collection tank 130.

[0053] Please refer to Figures 4A and 4B for schematic diagrams of the insert 180 shown in Figure 1. Insert 180 structurally comprises a plate-like member 182 and a plurality of tubular elements 184 arranged in a row on plate-like member 182. Also referring to Figure 5, the plurality of tubular elements 184 on insert 180 are positioned corresponding to the reaction wells 124 of base 120. These tubular elements 184 are configured to penetrate the inspection holes 164 of upper cover 160 and be inserted into the reaction wells 124 of base 120, thereby retaining the sample fluid within these reaction wells 124 within these tubular elements 184. Furthermore, it should be noted that the present invention, through the provision of insert 180, can retain the sample fluid within tubular elements 184 within these tubular elements 184, maintaining the sample within each reaction well 124 at the same height, thereby preventing sample loss, reducing contamination, and improving the accuracy of biological sample testing.

[0054] Figures 6A through 6C illustrate a microfluidic cartridge 7000 according to various embodiments of the present invention. In these figures, the microfluidic cartridge 7000 includes a reaction chamber cover and / or a sample cover for sealing the cartridge. For example, this arrangement allows the microfluidic cartridge 7000 to store reaction reagents prior to reaction, preventing spillage or contamination.

[0055] Please refer to FIG. 6A . The microfluidic cartridge 7000 further includes a reaction tank cover 190 and / or a sample cover 192 detachably covering the inspection hole 164 and the injection hole 162 of the cover 160 (see also FIG. 3A ).

[0056] Furthermore, in the embodiment of FIG. 6B , the sample cover is replaced with a sticker, and the test hole 164 is sealed with the sticker 193. In actual use, when the microfluidic cartridge 7000 is to be used for testing, the sticker 193 can be removed to expose the reaction hole 164, and the microfluidic cartridge 7000 of the present invention can then be used for testing.

[0057] Furthermore, in the embodiment of FIG. 6C , the reaction tank cover 190 is a sliding cover. The reaction tank cover 190 is movably engaged with the test cassette 100 and moves along the length of the test cassette 100. Those skilled in the art will appreciate that the configuration of the sliding cover can be modified based on actual usage requirements and common knowledge in the art.

[0058] Another aspect of the present invention is a method for performing a test using any of the microfluidic cartridges described in any embodiment of the present invention. Referring to Figures 7A to 7E , this embodiment depicts a method for performing a test using the microfluidic cartridge 1000 shown in Figure 1 . First, as shown in Figure 7A , a sample fluid S to be tested (e.g., a diluted or pre-treated liquid test sample) is injected into the test cartridge 100 through the injection port 162 . The sample fluid S then flows through the receiving reservoir 122 , through the drainage channel 126 , into the reaction reservoir 124 , and then through the indicator reservoir 128 into the collection reservoir 130 (see also Figures 2A and 2B ).

[0059] In addition, whether the test sample volume in the microfluidic cartridge 1000 is sufficient for the test can also be confirmed through the viewing window 104 to confirm whether the sample volume in each reaction tank 124 is sufficient. The sample volume can be confirmed by the naked eye or by related instruments.

[0060] Next, referring to Figures 7B to 7D , the insert 180 is inserted into the test cassette 100. The tubular members 184 penetrate the test holes 164 of the upper cover 160 and are inserted into the reaction wells 124 of the base 120 , thereby retaining the sample fluid S within the reaction wells 124 within the tubular members 184 (see also Figure 3 ). Referring to Figure 7D , when the insert 180 is inserted into the test cassette 100, it ensures that the sample fluid S within the test cassette 100 is prevented from escaping.

[0061] For example, during the testing process, an instrument is usually required to interpret the test results. In order to prevent the microfluidic cartridge 100 from shaking and causing the sample fluid S to flow out of the reaction tank 124, resulting in different test sample volumes in each reaction hole, thereby affecting the accuracy of the test, the present invention uses the configuration of the plug 180 to allow the sample fluid S to be retained in the reaction tank 124, and the sample fluid S in each reaction tank 124 can maintain a certain volume, and each sample fluid S is maintained at the same height, thereby maintaining stability during biological sample testing.

[0062] In this step, the microfluidic cartridge 1000 of the present invention can be used in conjunction with existing testing methods in the technical field of the present invention. For example, after inserting the plug-in 180 into the test cartridge 100, the instrument can be used to perform a nucleic acid amplification reaction first, and then the assay can be performed using enzyme immunoassay, fluorescence immunoassay, nucleic acid fluorescence or colorimetric assay.

[0063] In addition, the sample cover 192 can be placed on the injection hole 162 as needed to prevent the test sample from splashing during the operation process, which may cause cross contamination between samples.

[0064] Finally, please refer to Figure 7E. The result after the reaction (such as a color reaction or a fluorescent reaction) can be read by the relevant testing equipment D through the through hole of the tubular object 184 of the plug 180 (i.e., the inspection hole 164 corresponding to the upper cover 160), for example, through the measurement of the absorbance value or the fluorescence value.

[0065] In one embodiment of the present invention, the reaction result can be detected by a fluorescence detection device to detect the fluorescence value of the sample fluid S in the tubes 184 .

[0066] The microfluidic cartridge proposed in the present invention is suitable for use in the fields of environmental testing or medical testing, especially in the field of medical testing. Those skilled in the art can design, coat, or externally place reaction substances (such as nucleic acid probes, antigens, antibodies, or other substances) in the reaction tank of the microfluidic cartridge according to actual usage requirements to facilitate the detection of the test substances in the sample fluid.

[0067] Although specific embodiments of the present invention are provided in the above embodiments, they are not intended to limit the present invention. Those skilled in the art can make various changes and modifications without departing from the principles and spirit of the present invention. Therefore, the scope of protection of the present invention shall be based on the scope defined in the accompanying patent applications.

Claims

1. A microfluidic cartridge, characterized in that: Used to process a sample fluid, including: A test box includes a base and an upper cover that are engaged with each other, wherein: The base comprises: a receiving tank, comprising a plurality of buffer flow channels, disposed on the base; A plurality of reaction tanks are disposed on the base and located downstream of the receiving tank; A plurality of drainage channels, one end of which is in fluid communication with the receiving tank and the other end of which is in fluid communication with the reaction tank, for guiding the sample fluid from the receiving tank to the reaction tank; A plurality of indicator grooves are respectively in fluid communication with the reaction groove, and are used to receive the sample fluid flowing out of the reaction groove; and a collecting tank, in fluid communication with the indicating tank, for receiving the sample fluid flowing out of the indicating tank; The upper cover is disposed on the base and comprises: An injection hole, located at the upper cover and corresponding to the receiving groove; a plurality of inspection holes, located on the upper cover and respectively corresponding to the reaction tanks; and a flow guide, located below the upper cover and corresponding to the buffer channel or the drainage channel of the receiving tank, and in fluid communication with the buffer channel or the drainage channel, for guiding the sample fluid from the receiving tank to the reaction tank; and A plug-in unit, used for being inserted into a plurality of reaction slots of the test cassette, comprising: a plate-like member; A plurality of tubular objects are arranged in a row on the plate-like member and are arranged corresponding to the reaction grooves, wherein the tubular objects are configured to penetrate the inspection holes of the upper cover and be inserted into the reaction groove of the base, so that the sample fluid in the reaction groove is retained in the tubular objects.

2. The microfluidic cartridge according to claim 1, wherein: Also includes: A first inclination angle is provided at the fluid connection point between each reaction tank and each indicator tank, and is inclined from each reaction tank to each indicator tank, so as to avoid The sample fluid flows back into each of the reaction tanks; as well as A second inclination angle is provided at the fluid connection point between each of the indicator grooves and the collecting groove, and is inclined from each of the indicator grooves toward the collecting groove, so as to prevent the sample fluid from flowing back into each of the indicator grooves.

3. The microfluidic cartridge according to claim 1, wherein: There are three reaction slots and three indicator slots respectively and they are arranged in a row, and wherein: The fluid connection point between the reaction tank and the corresponding indicator tank located in the middle of the reaction tank is fan-shaped.

4. The microfluidic cartridge according to claim 1, wherein: The invention also comprises a sample cover which can be covered on the injection hole.

5. The microfluidic cartridge according to claim 1, wherein: It also comprises a reaction tank upper cover, which is used for detachably covering the tubular object of the plug-in unit.

6. The microfluidic cartridge according to claim 1, wherein: It also includes at least one viewing window, which is arranged corresponding to the indicating groove.

7. The microfluidic cartridge according to claim 1, wherein: It also includes a plurality of anti-overflow rings, which are respectively arranged on the upper edges of the inspection holes.

8. The microfluidic cartridge according to claim 1, wherein: The bottom of the base corresponding to the plurality of reaction tanks is more protruding than the bottom around the base.

9. A method for testing using the microfluidic cartridge as claimed in claim 1, characterized in that: Include: (a) injecting a sample fluid into the base through the injection hole of the upper cover, wherein the sample fluid flows through the receiving slot through the drainage channel to the reaction slot, the indicator slot and the collection slot respectively; (b) inserting the plug-in through the upper cover into the base, wherein the tubular objects of the plug-in are respectively inserted into the reaction grooves of the base, so that the sample fluid is retained in the tubular objects; and (c) detecting the content of a test substance in the sample fluid in the tube.

10. The method according to claim 9, characterized in that In the step (c), a fluorescence value of the sample fluid in the tube is detected by a fluorescence detection device.

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