Dynamic polymerization hybrid crop breeding method

Abstract

Provided in the present invention is a dynamic polymerization hybrid crop breeding method. The method is not only applied to corn breeding, but is also applied to hybrid breeding of rice, sorghum, cotton, rapeseed, green pepper and other plants that can be used in male-sterility three-way cross breeding.

Description

A dynamic polymerization hybrid crop breeding method Technical Field The present invention relates to agriculture and biotechnology, in particular to crop breeding, propagation and seed production technology, and specifically to a hybrid breeding, propagation and seed production method for crops such as rice, corn, sorghum, cotton, rapeseed and green pepper. Background Art People have long discovered the phenomenon of hybrid vigor in crops and have widely used it in the production of crops such as corn, sorghum, rice, rapeseed, and green pepper. Strengthening the use of hybrid vigor, reducing seed production costs, and improving breeding efficiency are the research directions of crop hybrid breeding. Three-line hybrid breeding technology is a three-line hybrid breeding method using a male sterile line, a male sterile maintenance line, and a male sterile restorer line. With the widespread application of three-line hybrid breeding technology in the breeding of crops such as rice, sorghum, cotton, and rapeseed. As the third largest crop in China's planting area, most of the corn varieties bred at this stage are single-cross varieties. The use of male sterile lines for three-line hybrid breeding can eliminate artificial emasculation, reduce seed costs, and improve seed purity. Three-line hybrid breeding of corn has received widespread attention in the breeding field. Three-line hybrid breeding technology has achieved great success in crop breeding, especially in sorghum and rice breeding. However, since the breeding of new high-quality male sterile lines and male sterile maintenance lines generally requires 6-8 generations, and the male sterile restoration lines also need to go through multiple generations of self-pollination and breeding, the breeding cycle of new varieties is long, the investment is large, and the results are slow. New breeding technologies are urgently needed to shorten the breeding cycle, improve breeding efficiency, and reduce breeding costs. Technical issues The present invention aims to solve the difficult problems of long breeding cycle, high investment and slow effect of new variety selection and breeding, and to provide a crop breeding, propagation and seed production technology with short cycle, high efficiency and low cost. Technical Solutions The technical solution of the present invention is to use the male sterile line, the male sterile maintenance line and the male sterile restoration line of the crop to carry out dynamic polyhybridization. Beneficial Effects The beneficial effects of the present invention are as follows: 1. The method of the present invention can concentrate the excellent traits of different parental materials, perform effective aggregation and accumulation of genes, obtain excellent strains and combinations with more outstanding comprehensive trait performance, and significantly improve breeding efficiency. 2. The method of the present invention selects the male and female parents to prepare single-cross, three-cross, and double-cross varieties according to the principle of complementarity based on the genetic characteristics controlled by multiple resistance genes. The aggregation of resistance is achieved, and a series of multi-cross varieties with high yield, excellent quality, strong resistance, and wide adaptability are bred. 3. The method of the present invention uses a single-cross combination of a male sterile maintenance line and a male sterile line with good complementarity as the female parent, selects a hybrid combination that partially or completely restores the male sterile female parent as the male parent, and assembles double-cross varieties to achieve multiple utilization of hybrid advantages, shorten the breeding cycle, reduce the cost of breeding, propagation, and seed production, and improve the breeding, propagation, and seed production efficiency of crops. 4. The present invention provides a dynamic polymerization hybrid crop breeding method, which is used to breed new varieties of crops such as corn, rice, sorghum, cotton, rapeseed, and green pepper, so as to achieve the polymerization accumulation of multiple excellent traits such as high yield, high quality, multi-resistance, and adaptability, and multiple utilization of hybrid advantages, significantly improve crop breeding efficiency, and achieve high yield of breeding and seed production. The method of the present invention includes but is not limited to the breeding of hybrids of crops such as corn, sorghum, rice, cotton, rapeseed, and green pepper. BRIEF DESCRIPTION OF THE DRAWINGS Type your figure caption description paragraph here. Best Mode for Carrying Out the Invention The technical solution provided by the present invention is as follows: The dynamic polymerization hybrid crop breeding method is characterized by the following specific steps: (1) using the male sterile line An as the female parent and the male sterile different cell nucleus series maintenance line Bn as the male parent, combining the series sterile single cross combination An*Bn, and selecting the sterile single cross combination ZAn with good comprehensive traits. This step is to breed the male sterile hybrid female parent, and is also the selection of the male sterile line An paired with the maintenance line Ban and the maintenance line Bn single cross combination Ban*Bn or Bn*Ban single cross varieties. (2) Using the male sterile line An1 as the female parent and the restorer line Rn as the male parent, a series of single-cross combinations are assembled, and a fertile single-cross combination An1*Rn with good comprehensive traits is selected. This step is to breed a multi-cross male fertile hybrid male parent, and is also the selection of a three-line single-cross of the male sterile line An1 and the restorer line Rn. Using male sterile lines for three-line hybrid breeding can avoid artificial emasculation, reduce seed costs, and improve seed purity. (3) Using the male sterile restorer line Rn1 as the female parent and the male sterile restorer line Rn2 as the male parent, a series of fertile single-cross combinations are assembled, and the fertile single-cross combination Rn1*Rn2 with good comprehensive traits is selected. This step is to prepare the restorer hybrid male parent of the multi-cross variety, and is also the selection of the restorer single-cross variety. (4) Using the male sterile single cross combination ZAn prepared in step (1) as the female parent and the male sterile restoration series restorer line Rn1 as the male parent for hybridization, a male sterile fully restored three-cross variety ZAn*Rn1[An*Bn / Rn1] is obtained; or using the conventional single cross combination Ban*Bn or Bn*Ban described in step (1) as the female parent and the male sterile restoration series restorer line Rn1 as the male parent for hybridization, a conventional fertile restoration and maintenance mixed type three-cross variety Ban*Bn / Rn1 or Bn*Ban / Rn1 is obtained. Both have the following characteristics: high yield, excellent quality, strong resistance, wide adaptability, and good comprehensive traits. (5) The sterile single cross combination ZAn with good comprehensive traits selected in step (1) is used as the female parent, and the restorative single cross combination Rn1*Rn2 in step (3) is used as the male parent for hybridization to obtain the male sterile fully restored double cross variety ZAn / Rn1*Rn2 [An*Bn / Rn1*Rn2]. It has the following characteristics: high yield, excellent quality, strong resistance, wide adaptability, good comprehensive traits, high yield, and the like. This step is the reuse of hybrid vigor. (6) The sterile single cross combination ZAn with good comprehensive traits selected in step (1) is used as the female parent, and the male sterile single cross combination An1*Rn restored in step (2) is used as the male parent for hybridization to obtain the male sterile partially restored double cross variety ZAn / An1*Rn. It has the following characteristics: high yield, excellent quality, strong resistance, wide adaptability, good comprehensive traits, high yield, high seed production, etc. This step is the reuse of hybrid vigor. The series of male sterile lines An and An1 described in step (1) and step (2) are paired male sterile lines and male sterile maintenance lines, An-Ban and An1-Ban1, which are formed by backcrossing male sterile line material A and maintenance material B for 8 generations. The quality, disease resistance and adaptability of An and An1 are consistent with those of the recurrent parents Ban and Ban1, and the specific characteristics are: infertility, stable sterility, excellent quality, wide adaptability and good comprehensive traits; the sterile single cross combination An*Bn and the recovery single cross combination An1*Rn described in step (1) and step (2) are respectively the female parent and the male parent for preparing double cross seeds exempted from artificial emasculation, and both have the following characteristics: high yield, excellent quality, strong resistance, wide adaptability, good comprehensive traits, and advantages such as high breeding and high seed production yield. The series of male sterility restoration lines Rn, Rn1, and Rn2 described in step (2) and step (3) are all male sterility restoration lines bred by hybridization between restoration materials R and self-pollination for multiple generations, and have the following specific characteristics: strong male sterility restoration, excellent quality, wide adaptability, and good comprehensive traits; the restoration single-cross combination Rn1*Rn2 described in step (3) is the male parent for preparing double-cross seeds, and has the following characteristics: stable restoration, strong resistance, excellent quality, wide adaptability, good comprehensive traits, high number of seeds, and high seed production yield. Embodiments of the present invention Specific implementation scheme: A dynamic polymerized hybrid corn breeding method, the corn male sterility maintenance line Ban with good comprehensive traits in step (1) includes W360B, W240B, W382B, 106B, f16B and other advanced male sterility maintenance lines, all of which are bred by multi-generation self-pollination using various male sterility maintenance type original resources. The corn male sterility line An with good comprehensive traits in step (1) includes W360A, W240A, W382A, 106A, F16A and other male sterility lines, which are bred by multi-generation backcrossing, and its quality, disease resistance, and comprehensive trait performance are consistent with those of the recurrent parents, and the specific characteristics are: stable male sterility and good comprehensive traits. The sterile single cross combination ZAn[An*Bn] with good comprehensive traits described in step (1) is the female parent for preparing the three-cross and double-cross varieties of corn: ZA1[W360A*2052B], ZA2[W360A*2058B], and ZA3[W240A*2058B], which exhibits stable male sterility and strong hybrid vigor. This step is the first use of hybrid vigor. The series of corn male sterility restorer lines Rn[W98, T0203, 21JT-2], Rn1[T0759], Rn2[198, 270] described in step (2) and step (3) are all male sterility restorer lines bred by hybridization between restorer materials R and self-pollination for multiple generations. The restorative single cross combination Rn1*Rn2[T0759*198, T0759*270] described in step (3) is the male parent for preparing the fully restorative double cross seed, and has the following characteristics: stable recovery, strong resistance, excellent quality, wide adaptability, good comprehensive traits, and high seed production yield. In step (2), the male sterile line An1[F16A] is used as the female parent to restore the male sterile restorer line Rn

[0198] As the male parent, the fertile single-cross combination An1*Rn[F16A*198] is combined, and it is the male parent for preparing partially restored double-cross varieties. It has the advantages of strong comprehensive resistance, high reproduction and seed production yield, etc. In step (4), the sterile single cross combination ZAn is used as the female parent and the male sterile restoration series restorer line Rn1 is used as the male parent for hybridization to obtain the male sterile fully restored three-cross variety ZAn*Rn1[An*Bn / Rn1]:[W360A*2058B / T0203], [W240A*2058B / W98], which has the following specific characteristics: full fertility restoration, stable recovery, high yield, excellent quality, strong resistance, wide adaptability and good comprehensive traits. In step (5) and step (6), the sterile single cross combination ZAn is used as the female parent, and the partially restored male sterile single cross combination An1*Rn and the fully restored male sterile single cross combination Rn1*Rn2 are used as the male parents for hybridization, respectively, to obtain double cross varieties ZAn / An1*Rn[W360A*2052B / F16A*198].ZAn / Rn1*Rn2[An*Bn / Rn1*Rn2][W360A*2058B / T0759*198], [W360A*2058B / T0759*270], which are double cross varieties exempted from artificial emasculation, and have the following characteristics: high yield, excellent quality, strong resistance, wide adaptability, good comprehensive traits, and advantages of high fertility and high seed production yield. This step is the reuse of hybrid vigor. Industrial Applicability Type your industrial applicability description paragraph here. Sequence Listing Free Content Type your sequence listing free description paragraph here.

Claims

1. The present invention is a method for breeding crops by dynamic polymerization hybridization. The method is applied to the hybridization and selection of crops such as corn, rice, sorghum, cotton, rapeseed, and green pepper. It is a breeding method that aggregates multiple excellent traits such as high yield, high quality, multi-resistance, and wide adaptability, and utilizes hybrid advantages multiple times. It significantly improves the efficiency of crop breeding, and has high yields in breeding and seed production. The technical solution provided by the present invention: The method for breeding crops by dynamic polymerization hybridization is characterized in that The specific steps are as follows: (1) Using the male sterile line An as the female parent and the male sterile different cell nucleus series maintenance line Bn as the male parent, the series sterile single cross combination An*Bn is matched, and the sterile single cross combination ZAn with good comprehensive traits is selected. This step is to breed male sterile hybrid female parents, and is also the breeding of male sterile line An paired with maintenance line Ban and maintenance line Bn single cross combination Ban*Bn or Bn*Ban single cross varieties. (2) Using the male sterile line An1 as the female parent and the male sterile series restorer line Rn as the male parent, the series single cross combination is matched, and the fertile single cross combination An1*Rn with good comprehensive traits is selected. This step is to breed multi-cross male fertile hybrid male parents, and is also the breeding of three-line single cross varieties of male sterile line An1 and restorer line Rn. The use of male sterile lines for three-line hybrid breeding can avoid artificial emasculation, reduce seed costs, and improve seed purity. (3) With the male sterile restorer line Rn1 as the female parent and the male sterile restorer line Rn2 as the male parent, a series of fertile single cross combinations are assembled, and the fertile single cross combination Rn1*Rn2 with good comprehensive traits is selected. This step is to prepare the restorer hybrid male parent of the multi-cross variety, and is also the selection of the restorer single cross variety. (4) With the male sterile single cross combination ZAn assembled in step (1) as the female parent and the restorer line Rn1 of the male sterile series as the male parent, hybridization is performed to obtain the male sterile fully restored three-cross variety ZAn*Rn1[An*Bn / Rn1] or with the conventional single cross combination Ban*Bn or Bn*Ban described in step (1) as the female parent and the restorer line Rn1 of the male sterile series as the male parent, hybridization is performed to obtain the conventional fertile restorer and maintainer mixed three-cross variety Ban*Bn / Rn1 or Bn*Ban / Rn1. All of them have the following characteristics: high yield, good quality, strong resistance, wide adaptability, and good comprehensive traits. (5) The sterile single-cross combination ZAn with good comprehensive traits selected in step (1) is used as the female parent, and the single-cross combination Rn1*Rn2 with recovery in step (3) is used as the male parent for hybridization to obtain the male sterile full recovery double-cross variety ZAn / Rn1*Rn2 [An*Bn / / Rn1*Rn2], which has the following characteristics: high yield, good quality, strong resistance, wide adaptability, good comprehensive traits, and advantages such as high fertility and high seed production yield. This step is the reuse of hybrid vigor. (6) The sterile single-cross combination ZAn with good comprehensive traits selected in step (1) is used as the female parent, and the male sterile single-cross combination An1*Rn restored in step (2) is used as the male parent for hybridization to obtain a male sterile partially restored double-cross variety ZAn / An1*Rn, which has the following characteristics: high yield, excellent quality, strong resistance, wide adaptability, good comprehensive traits, high fertility, high seed production yield, etc. This step is the reuse of hybrid vigor.

2. The method for breeding crops by dynamic polymerization hybridization according to claim 1, characterized in that: The maize male sterile line An[W360A, W240A, W382A, 106A, F16A] described in step (1) and step (2) is bred by backcrossing of the paired maintenance line Ban[W360B, W240B, W382B, 106B, f16B] for multiple generations, and its quality, disease resistance, and comprehensive trait performance are consistent with those of the recurrent parent, and the specific characteristics are: stable male sterility; the maize male sterile series restorer lines Rn[W98, T0203, 21JT-2], Rn1[T0759], and Rn2[198, 270] are all male sterile restorer lines bred by hybridization between restorer materials R for multiple generations of self-pollination, and the specific characteristics are: stable male sterility restoration.

3. The method for breeding crops by dynamic polymerization hybridization according to claim 1, characterized in that: The male sterile single cross combination An*Bn in step (1) is selected from the sterile single cross combination ZAn: ZA1[W360A*2052B], ZA2[W360A*2058B], and ZA3[W240A*2058B] with good comprehensive traits, which is the female parent for preparing male sterile double cross varieties; the fertile single cross combination An1*Rn[F16A*198] in step (2) is selected from the fertile single cross combination Rn1*Rn2 in step (3), and the series combination shows strong hybrid superiority, and the fertile single cross combination Rn1*Rn2[T0759*198, T0759*270] with good comprehensive traits is selected from ... This step is a one-time utilization of hybrid advantage when preparing multi-hybrid male and female parents.

4. The method for breeding crops by dynamic polymerization hybridization according to claim 1, characterized in that: In step (1), the sterile single cross combination ZAn is used as the female parent, and the male sterile restoration series restorer line Rn1 is used as the male parent for hybridization to obtain the male sterile fully restored three-cross variety ZAn*Rn1[An*Bn / Rn1]:[W360A*2058B / T0203], [W240A*2058B / W98], which has the following characteristics: high yield, excellent quality, strong resistance, wide adaptability, good comprehensive traits, and high seed production yield. This step is the reuse of hybrid vigor.

5. The method for breeding crops by dynamic polymerization hybridization according to claim 1, characterized in that: The male sterile single cross combination An*Bn assembled in step (5) is selected as the female parent, and the sterile single cross combination ZAn with good comprehensive traits is selected as the male parent, and the male sterile single cross combination Rn1*Rn2 restored in step (3) is used as the male parent for hybridization to obtain the male sterile fully restored double cross seed ZAn / Rn1*Rn2[An*Bn / Rn1*Rn2]:[W360A*2052B / T0759*198], [W360A*2058B / / T0759*270], which has the following characteristics: high yield, excellent quality, strong resistance, wide adaptability, good comprehensive traits, and high seed production yield. This step is the reuse of hybrid vigor.

6. The method for breeding crops by dynamic polymerization hybridization according to claim 1, characterized in that: The male sterile single cross combination An*Bn assembled in step (6) is selected as the female parent of the sterile single cross combination ZAn with good comprehensive traits, and the male fertile single cross combination An1*Rn described in step (2) is used as the male parent for hybridization to obtain the male sterile partial recovery double cross variety ZAn / An1*Rn[W360A*2052B / F16B*198], which has the following characteristics: high yield, excellent quality, strong resistance, wide adaptability, good comprehensive traits, and high seed production yield. This step is the reuse of hybrid vigor.

7. The dynamic polymerization hybrid crop breeding method according to claim 1 is not only used in corn breeding, but also in hybrid breeding of rice, sorghum, cotton, rapeseed, green pepper, and other plants that can be used for male sterile three-line hybrid breeding.

8. The parental lines, rotary lines and hybrid combinations obtained by the method of the present invention all fall within the protection scope of the present invention.

9. The plant breeding method claimed in the present invention may include steps (1) to (6) as described above.

10. The present invention claims protection for a dynamic polymer hybrid plant breeding method, and the new varieties cultivated using this method also fall within the scope of protection of the present invention.