Gene sequencing system and method, device, and non-volatile storage medium

Abstract

Provided are a gene sequencing system and method, a device, and a non-volatile storage medium. The system comprises: a control apparatus, a first biochemical reaction and detection platform, and a second biochemical reaction and detection platform, wherein the control apparatus is used for controlling the first biochemical reaction and detection platform and the second biochemical reaction and detection platform to respectively execute synchronous or asynchronous sequencing processes. The technical problems in the related art of large size and long sequencing time of gene sequencing systems due to the need for two platforms to respectively perform biochemical reaction and signal detection on a sequencing slide during gene sequencing are solved.

Description

Gene sequencing system, method, device and non-volatile storage medium Technical Field

[0001] The present disclosure relates to the biological field, and in particular, to a gene sequencing system, method, device and non-volatile storage medium. Background Art

[0002] Gene sequencing systems typically consist of a series of components, including mechanics, electronics, fluidics, temperature control, biochemistry, slides, motion control, and optical systems, all working together to achieve gene sequencing. Due to their inherent complexity, gene sequencing systems are large in size, lack flexibility, and require a long overall sequencing time.

[0003] Related technologies usually adopt a design scheme that supports sequencing of multiple samples by the sequencing system to improve its flexibility of use and the efficiency of gene sequencing. However, the sequencing carriers need to perform biochemical reactions and signal detection on the biochemical reaction platform and the detection platform respectively, and the two platforms cannot be reused. At the same time, transfer systems, robots, transfer tables, core drives and other devices are introduced to realize the transfer of sequencing carriers between the biochemical reaction platform and the detection platform. The robot needs a certain amount of space to move the sequencing carriers, which leads to the problem of large size and high cost of the gene sequencing system.

[0004] To address the above-mentioned problems, no effective solutions have been proposed so far.

[0005] Summary of the Invention

[0006] The embodiments of the present disclosure provide a gene sequencing system, method, device, and non-volatile storage medium to at least address the technical issues of large gene sequencing system size and long sequencing time caused by the fact that gene sequencing systems in related technologies require two platforms to perform biochemical reactions and signal detection on sequencing slides respectively when performing gene sequencing.

[0007] According to one aspect of an embodiment of the present disclosure, a gene sequencing system is provided, including: a control device, a first biochemical reaction and detection platform, and a second biochemical reaction and detection platform, wherein the control device is used to control the first biochemical reaction and detection platform and the second biochemical reaction and detection platform to respectively perform synchronous or asynchronous sequencing processes.

[0008] According to another aspect of an embodiment of the present disclosure, a gene sequencing method is also provided, including: obtaining the number of sequencing slides to be sequenced; determining a sequencing process based on the number of sequencing slides, wherein the sequencing process is used to control a first biochemical reaction and detection platform and a second biochemical reaction and detection platform to respectively perform a synchronous or asynchronous sequencing process through a control device in a gene sequencing system, and the gene sequencing system includes a control device, a first biochemical reaction and detection platform, and a second biochemical reaction and detection platform; executing a first sequencing process when the number of sequencing slides is 1, executing a second sequencing process when the number of sequencing slides is greater than 1 and the sequencing slides are sequenced simultaneously, and executing a third sequencing process when the number of sequencing slides is greater than 1 and the sequencing slides are not sequenced simultaneously.

[0009] According to another aspect of the embodiments of the present disclosure, a gene sequencing device is provided, including: a memory for storing program instructions; and a processor connected to the memory for executing the above-mentioned gene sequencing method.

[0010] According to another aspect of the embodiments of the present disclosure, a non-volatile storage medium is provided. The non-volatile storage medium includes a stored computer program, wherein the device where the non-volatile storage medium is located executes the above-mentioned gene sequencing method by running the computer program.

[0011] According to another aspect of the embodiments of the present disclosure, a computer program product is provided, comprising computer instructions, which implement the above-mentioned gene sequencing method when executed by a processor.

[0012] In an embodiment of the present disclosure, a gene sequencing system includes a control device, a first biochemical reaction and detection platform, and a second biochemical reaction and detection platform. The control device is used to control the first biochemical reaction and detection platform and the second biochemical reaction and detection platform to respectively perform synchronous or asynchronous sequencing processes, thereby achieving the purpose of performing biochemical reactions and information detection on sequencing slides on the same platform without the need to transfer sequencing slides between the biochemical reaction platform and the signal detection platform, thereby achieving the technical effect of accelerating the overall sequencing time and reducing the overall cost and volume of the gene sequencing system, thereby solving the technical problem of large gene sequencing system volume and long sequencing time caused by the gene sequencing system in the related art requiring two platforms to perform biochemical reactions and signal detection on the sequencing slides respectively when performing gene sequencing. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] The drawings described herein are used to provide a further understanding of the present disclosure and constitute a part of the present disclosure. The exemplary embodiments of the present disclosure and their descriptions are used to explain the present disclosure and do not constitute an improper limitation of the present disclosure. In the drawings:

[0014] FIG1 is a schematic diagram of the appearance of a gene sequencing system according to an embodiment of the present disclosure;

[0015] FIG2 is a structural diagram of a gene sequencing system according to an embodiment of the present disclosure;

[0016] FIG3 is a diagram of the internal structure of a gene sequencing system according to an embodiment of the present disclosure;

[0017] FIG4 is a flow chart of a first sequencing process according to an embodiment of the present disclosure;

[0018] FIG5a is a flowchart of the first part of a second sequencing process according to an embodiment of the present disclosure;

[0019] FIG5 b is a flowchart of the second part of the second sequencing process according to an embodiment of the present disclosure;

[0020] FIG6 a is a flowchart of the first part of a third sequencing process according to an embodiment of the present disclosure;

[0021] FIG6 b is a flowchart of the second part of the third sequencing process according to an embodiment of the present disclosure;

[0022] FIG7 is a flow chart of a gene sequencing method according to an embodiment of the present disclosure;

[0023] FIG8 is a hardware structure diagram of a computer terminal for implementing a gene sequencing method according to an embodiment of the present disclosure. DETAILED DESCRIPTION

[0024] In order to enable those skilled in the art to better understand the solutions of the present disclosure, the technical solutions in the embodiments of the present disclosure will be clearly and completely described below in conjunction with the drawings in the embodiments of the present disclosure. Obviously, the embodiments described are only part of the embodiments of the present disclosure, not all of the embodiments. Based on the embodiments of the present disclosure, all other embodiments obtained by ordinary technicians in this field without making creative efforts should fall within the scope of protection of the present disclosure.

[0025] It should be noted that the terms "first", "second", etc. in the specification and claims of the present disclosure and the above-mentioned drawings are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that the numbers used in this way can be interchanged where appropriate, so that the embodiments of the present disclosure described herein can be implemented in an order other than those illustrated or described herein. In addition, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusions, for example, a process, method, system, product or device that includes a series of steps or units is not necessarily limited to those steps or units clearly listed, but may include other steps or units that are not clearly listed or inherent to these processes, methods, products or devices.

[0026] The present disclosure provides a schematic diagram of the appearance, internal structure, and system structure of a gene sequencing system. The following first introduces the appearance of the gene sequencing system, specifically as follows:

[0027] Figure 1 is a schematic diagram of the appearance of a gene sequencing system according to an embodiment of the present disclosure. As shown in Figure 1, when its lifting screen 102 is in the descending state 1a, the gene sequencing system includes a shell 101, a lifting screen 102, a waste liquid compartment door A103 and a waste liquid compartment door B104, wherein the lifting screen 102 is touchable; when its lifting screen 102 is in the ascending state 1b, the gene sequencing system receives sequencing slides through the sample insertion interface A105 and the sample insertion interface B106, and receives the sequencing reagent tank through the reagent insertion interface A107 and the reagent insertion interface B108. The sequencing system uses the sequencing reagents in the sequencing reagent tank to analyze one or more biological characteristics of the sample in the sequencing slide, thereby achieving gene sequencing.

[0028] FIG2 is a structural diagram of a gene sequencing system according to an embodiment of the present disclosure, and FIG3 is an internal structural diagram of a gene sequencing system according to an embodiment of the present disclosure. The gene sequencing system will be described below in conjunction with FIG2 and FIG3 .

[0029] In Figure 2 , the gene sequencing system includes a control device 208, a first biochemical reaction and detection platform 202, and a second biochemical reaction and detection platform 204. Control device 208 is configured to control the first and second biochemical reaction and detection platforms to perform synchronous or asynchronous sequencing, respectively. The gene sequencing system also includes a data acquisition module 206, which is configured to collect data from sequencing slides after biochemical reactions on the first or second biochemical reaction and detection platforms have been performed, after the control device moves the first or second biochemical reaction and detection platform to a sampling position.

[0030] In one embodiment of the present disclosure, the control device 208 in FIG. 2 is referred to as the electronic and motion control device in FIG. 3 . The first biochemical reaction and detection platform 202 and the second biochemical reaction and detection platform 204 in FIG. 2 correspond to biochemical reaction and detection platform A 202 and biochemical reaction and detection platform B 204, respectively, in FIG. 3 . The first and second biochemical reaction and detection platforms are collectively referred to as the biochemical reaction and detection platforms. The data acquisition module 206 in FIG. 2 includes an objective lens, a camera, an autofocus module, and a defined Z-axis in FIG. 3 . This data acquisition module 206 is used to focus and capture images of sequencing slides during the signal detection phase. The synchronous sequencing process described above refers to a process in which multiple sequencing slides are sequenced simultaneously, for example, two sequencing slides are sequenced simultaneously; the asynchronous sequencing process refers to a process in which multiple sequencing slides are sequenced asynchronously, for example, two sequencing slides are sequenced asynchronously. It should be noted that the number of biochemical reaction and detection platforms can be set according to actual needs. This is merely an example and does not limit the number of biochemical reaction and detection platforms.

[0031] Through this gene sequencing system, the purpose of performing biochemical reactions and information detection on sequencing slides can be achieved on the same platform, without having to transfer the sequencing slides between the biochemical reaction platform and the signal detection platform, thereby speeding up the overall sequencing time and reducing the overall cost and volume of the gene sequencing system. It solves the technical problem of large gene sequencing system volume and long sequencing time caused by the fact that gene sequencing systems in related technologies require two platforms to perform biochemical reactions and signal detection on sequencing slides respectively when performing gene sequencing.

[0032] Optionally, the gene sequencing system in Figure 2 also includes an XY platform 201, and the first biochemical reaction and detection platform 202 and the second biochemical reaction and detection platform 204 are set on the XY platform 201, wherein the XY platform 201 is used to move within a preset range of the XY coordinate system.

[0033] In the disclosed embodiment, XY platform 201 in FIG. 2 is also the XY platform in FIG. 3 , which is a motion control platform used to move within a preset travel range of an XY coordinate system. Biochemical reaction and detection platform A 202 and biochemical reaction and detection platform B 204 are disposed on XY platform 201. It should be noted that when there are multiple biochemical reaction and detection platforms, these platforms are disposed on the same XY platform 201.

[0034] Optionally, the biochemical reaction and detection platform includes a fluid device and a fixture, i.e., both the first biochemical reaction and detection platform and the second biochemical reaction and detection platform include a fluid device and a fixture. For ease of description, the fluid device and fixture in the first biochemical reaction and detection platform are referred to as a first fluid device and a first fixture, and the fluid device and fixture in the second biochemical reaction and detection platform are referred to as a second fluid device and a second fixture. The fluid device is used to pump reagents required for the biochemical reaction from the sequencing reagent tank into the sequencing slide, and the fixture is used to secure the sequencing slide placed on the biochemical reaction and detection platform. The fluid device, the fixture, and the sequencing slide are connected via an internal flow channel.

[0035] In one embodiment of the present disclosure, the fluidic device (or first fluidic device) in biochemical reaction and detection platform A202 is referred to as reagent chamber A and fluidic device A2021 in FIG3 , and the fixture (or first fixture) in biochemical reaction and detection platform A202 is referred to as sequencing slide fixture A2022 in FIG3 . The fluidic device (or second fluidic device) in biochemical reaction and detection platform B204 is referred to as reagent chamber B and fluidic device B2041 in FIG3 , and the fixture (or second fixture) in biochemical reaction and detection platform B204 is referred to as sequencing slide fixture B2042 in FIG3 .

[0036] It should be noted that in Figure 3, before using biochemical reaction and detection platform A202 for gene sequencing, the user places sequencing slide A203 on biochemical reaction and detection platform A202. Internal flow channels connect sequencing slide A203, sequencing slide fixture A2022, reagent reservoir A, and fluidic device A2021. Before using biochemical reaction and detection platform B204 for gene sequencing, the user places sequencing slide B205 on biochemical reaction and detection platform B204. Internal flow channels connect sequencing slide B205, sequencing slide fixture B2042, reagent reservoir B, and fluidic device B2041.

[0037] Optionally, the gene sequencing system in FIG2 further includes a computer 207 , which is connected to the control device 208 and the data acquisition module 206 , respectively, for sending control instructions to the control device and obtaining data information from the control device.

[0038] In the embodiment of the present disclosure, the computer 207 in FIG. 2 is also the computer in FIG. 3 , and is used to control and schedule the gene sequencing system by sending instructions, such as sending instructions to the control device 208 and the data acquisition module 206 , including acquiring images from the camera in the data acquisition module and acquiring data information such as device status from the control device 208 .

[0039] Optionally, the control device 208 in FIG. 2 is configured to receive control instructions, control the data acquisition module 206 according to the control instructions, and control the fluid device and the fixing device in the first biochemical reaction and detection platform or the second biochemical reaction and detection platform.

[0040] In the disclosed embodiment, the control device 208 receives control instructions from the computer 207 and distributes these instructions to the fluidic devices and fixtures in the first biochemical reaction and detection platform and / or the second biochemical reaction and detection platform. For example, in FIG3 , the control instructions may be distributed to the reagent reservoir A and fluidic device A 2021, the sequencing slide fixture A 2022, the reagent reservoir B and fluidic device B 2041, and the sequencing slide fixture B 2042. The control device 208 also distributes these control instructions to the data acquisition module 206, thereby controlling the objective lens, camera, autofocus module, and Z-axis. Simultaneously, the control device 208 obtains relevant status information from these other devices and uploads it to the computer 207.

[0041] Optionally, the computer 207 in the above-mentioned gene sequencing system is also used to obtain the number of sequencing carriers to be sequenced; determine the sequencing process based on the number of sequencing carriers, wherein when the number of sequencing carriers is 1, it corresponds to the first sequencing process; when the number of sequencing carriers is greater than 1 and the sequencing carriers are sequenced simultaneously, it corresponds to the second sequencing process; when the number of sequencing carriers is greater than 1 and the sequencing carriers are sequenced at different times, it corresponds to the third sequencing process.

[0042] In some embodiments, the third sequencing process can be implemented in a rolling fashion alongside the second sequencing process. Rolling onboarding refers to a method that relies on scheduling methods, either through software or software and hardware collaboration, to provide users with multiple independent service execution paths while providing partially redundant equipment rather than a fully redundant system. Furthermore, rolling onboarding can be defined as an efficient scheduling strategy that allows for dynamic management of sequencing equipment and processing of multiple sequencing processes through the collaborative work of software and hardware, without impacting the currently executing sequencing process, thereby improving the throughput and resource utilization of the sequencing system. For example, if a single sequencing system is perceived by the user as implementing a single service pipeline, rolling onboarding allows the user to perceive the system as implementing two or more service pipelines without requiring the use of two or more sequencing systems. For example, a gene sequencer is currently analyzing a set of DNA samples, i.e., sequencing process A. Simultaneously, the scheduling device learns that another set of samples is ready to be run onboard for sequencing process B, and that the current execution of sequencing process A is about to reach an interruptible point. Without even learning or interrupting any of these points, the scheduling device immediately directs the next set of samples to be run onboard. Alternatively, if the scheduling device learns that sequencing process A is about to reach an interruptible point, it will pause and save the state of sequencing process A. It will then quickly switch to loading another set of samples and begin sequencing process A and B synchronously or asynchronously. This rolling loading strategy ensures maximum utilization of sequencing resources and improves sequencing efficiency.

[0043] In some embodiments of the present disclosure, taking two sequencing slides as an example, that is, taking sequencing slide A203 and sequencing slide B205 as an example, the gene sequencing system supports sequencing of any single slide of sequencing slide A203 and sequencing slide B205, sequencing of two slides at the same time, and rolling sequencing of two slides. Specifically: when the number of slides to be sequenced is 1, it corresponds to the first sequencing process, that is, sequencing of any single slide; when the number of slides to be sequenced is greater than 1 and the sequencing slides are sequenced at the same time, it corresponds to the second sequencing process, that is, sequencing of two slides at the same time; when the number of slides to be sequenced is greater than 1 and the sequencing slides are not sequenced at the same time, it corresponds to the third sequencing process, that is, rolling sequencing of two slides. The above sequencing schemes all use a specific scheduling method that combines software and hardware to make the sample undergo multiple cycles of biochemical reactions and signal acquisition inside the gene sequencing system to achieve gene sequencing of the sample. The following is an explanation.

[0044] Optionally, the first sequencing process includes: step S11, the computer receives sequencing information filled in by the target object, and controls the lifting and lowering of the lifting screen according to the sequencing information so that the target object is placed in the sequencing reagent tank and the sequencing slide in the gene sequencing system; step S12, the computer sends a first instruction to the fluid device in the first biochemical reaction and detection platform or the fluid device in the second biochemical reaction and detection platform through the control device, wherein the first instruction is used to control the fluid device in the first biochemical reaction and detection platform or the second biochemical reaction and detection platform (i.e., the first fluid device or the second fluid device) to pump the reagents required for the biochemical reaction from the sequencing reagent tank into the sequencing slide so that the sample to be tested undergoes a biochemical reaction and enters the current cycle of gene sequencing; within a preset time before the biochemical reaction is completed, the computer sends a second instruction to the XY stage in the gene sequencing system through the control device, wherein the second instruction is used to control the XY stage to move to a first position so that the area on the sequencing slide where signal detection is required is at the position of the objective lens Z Directly below the direction, the objective lens is in the data acquisition module; step S13, after the biochemical reaction is completed, the computer sends a third instruction to the data acquisition module through the control device, wherein the third instruction is used to achieve focusing and signal acquisition on the sequencing carrier.

[0045] Optionally, when there are multiple areas in the sequencing slide that require signal collection, the computer controls the XY platform to move successively through the second instruction so that the first target area is located directly below the objective lens in the Z direction, wherein the first target area is a plurality of areas in the sequencing slide that require signal collection.

[0046] Optionally, the first sequencing process also includes: after completing signal acquisition for all areas requiring signal acquisition in the sequencing slide, determining whether all cycles of the gene sequencing cycle are completed, wherein the gene sequencing cycle includes step S12 and step S13; if all cycles of the gene sequencing cycle are not completed, returning to step S12 to continue gene sequencing of the sample to be tested, and if all cycles of the gene sequencing cycle are completed, executing step S14; step S14, generating a sequencing result for the sequencing slide.

[0047] The first sequencing process described above is explained below with reference to FIG4 . FIG4 is a flow chart of the first sequencing process according to an embodiment of the present disclosure. As shown in FIG4 , the sequencing process for sequencing any single slide (taking the individual sequencing of sequencing slide A203 as an example) includes: a sequencing preparation stage S1001 , a biochemical reaction stage S1002 , a signal acquisition stage S1003 , and a sequencing result generation stage S1004 . Specifically:

[0048] Step S1001: The user fills in the sequencing information. After the computer 207 receives the sequencing information filled in by the target subject, it first controls the lifting screen 102 to rise according to the sequencing information. After the user places the sequencing reagent tank A in the reagent insertion interface A107, the lifting screen 102 descends and preloads the reagent. Then the lifting screen 102 rises. After the user places the sequencing slide A203 in the sample insertion interface A105, the lifting screen 102 descends, thereby completing the sequencing preparation and entering the biochemical reaction stage of step S1002.

[0049] Step S1002: The computer 207 sends a first instruction to the fluidic device A via the control device 208. After receiving the first instruction, the fluidic device A pumps the reagents required for the biochemical reaction from the sequencing reagent tank A into the sequencing slide A 203 via the internal flow channel, so that the sample to be tested undergoes a biochemical reaction. Within a preset time (e.g., 10 seconds) before the biochemical reaction is completed, the computer 207 sends a second instruction to the XY platform 201 via the control device 208. After receiving the second instruction, the XY platform 201 moves to the first position. For example, the control device 208 moves the XY platform 201 to a specific coordinate position (XY) in the xy coordinate system. A1 , Y A1 ) so that the area on the sequencing slide A203 where signal detection is required is directly below the objective lens in the Z direction, and the signal acquisition stage of step S1003 is entered.

[0050] Step S1003: After the biochemical reaction is completed, the computer 207 sends a third instruction to the data acquisition module 206 through the control device 208, and the objective lens moves in the Z direction via the Z axis to achieve focusing and signal acquisition on the sequencing slide A203.

[0051] It should be noted that one biochemical reaction corresponds to one cycle, and multiple signal acquisitions may be performed in the cycle. Specifically, when the sequencing slide A203 may contain one, two, or more regions that require signal detection (i.e., the first target region), multiple signal acquisitions are required in the cycle corresponding to this biochemical reaction. After each signal acquisition in the signal acquisition phase, it is necessary to determine whether signal acquisition of all regions in the current cycle has been completed. If signal acquisition of all regions in the current cycle has not been completed, the computer 207 moves the XY platform 201 to the next specific coordinate position (XY coordinate position) through the control device 208. A* , Y A* ) so that the next area on the sequencing slide A203 that needs to be detected for signal is directly below the objective lens in the Z direction, and the control device 208 sends a third instruction to the data acquisition module 206, so that the signal acquisition for the next area on the sequencing slide A203 that needs to be detected for signal is completed through the third instruction, and this cycle is completed after all areas on the sequencing slide A203 that need to be detected for signal have completed signal acquisition.

[0052] Step S1004: Gene sequencing of the sample is achieved through a cycle of biochemical reaction stage S1002 and signal acquisition stage S1003, generating a sequencing result for sequencing slide A203. Specifically, since multiple biochemical reactions are required throughout the entire gene sequencing process, each biochemical reaction corresponds to one cycle. After the current cycle completes signal acquisition from all regions on the sequencing slide requiring signal acquisition, a determination is made as to whether all cycles of the gene sequencing cycle have been completed. The gene sequencing cycle includes steps S1002 and S1003. If all cycles of the gene sequencing cycle have not been completed, the process returns to step S1002 to continue biochemical reactions on the sample to be tested. If all cycles of the gene sequencing cycle have been completed, a gene sequencing result for sequencing slide A203 is generated.

[0053] Optionally, the second sequencing process includes: step S21, the computer receives sequencing information filled in by the target object, and controls the lifting and lowering of the lifting screen according to the sequencing information so that the target object is placed in the sequencing reagent tank and the sequencing slide in the gene sequencing system; step S22, the computer sends a fourth instruction to the first fluid device in the first biochemical reaction and detection platform and the second fluid device in the second biochemical reaction and detection platform through the control device, wherein the fourth instruction is used to control the first fluid device and the second fluid device to pump the reagents required for the biochemical reaction from the sequencing reagent tank into the corresponding sequencing slide so that the sample to be tested undergoes a biochemical reaction and enters the gene sequencing cycle of the current cycle, and each fluid device corresponds to a sequencing slide; within a preset time before the biochemical reaction is completed, the computer detects the XY The system includes: a step of determining the occupation status of the platform; in a state where the XY platform is occupied by other sequencing carriers, after waiting for the XY platform to be released, the first sequencing carrier among the sequencing carriers occupies the XY platform, and the computer sends a fifth instruction to the XY platform in the gene sequencing system through the control device, wherein the fifth instruction is used to control the XY platform to move to the second position so that the area of ​​the first sequencing carrier requiring signal detection is directly below the objective lens in the Z direction, the objective lens is in the data acquisition module, and the first sequencing carrier is any one of the sequencing carriers corresponding to the first biochemical reaction and detection platform or the second biochemical reaction and detection platform; step S23, after the biochemical reaction is completed, the computer sends a sixth instruction to the data acquisition module through the control device, wherein the sixth instruction is used to achieve focusing and signal acquisition for the first sequencing carrier.

[0054] Optionally, when there are multiple areas in the first sequencing slide that require signal collection, the computer controls the XY platform to move successively through the fifth instruction so that the second target areas are successively located directly below the objective lens in the Z direction, wherein the second target areas are multiple areas in the first sequencing slide that require signal collection.

[0055] Optionally, the second sequencing process also includes: after completing signal acquisition for all areas requiring signal acquisition in the first sequencing carrier, releasing the XY platform, and determining whether all cycles of gene sequencing cycles are completed, wherein the gene sequencing cycle includes step S22 and step S23; if all cycles of gene sequencing cycles are not completed, returning to step S22 to continue gene sequencing of the sample to be tested in the first sequencing carrier, and if all cycles of gene sequencing cycles are completed, executing step S24; step S24, generating the sequencing result of the first sequencing carrier.

[0056] The second sequencing process is explained below with reference to FIG5a and FIG5b. FIG5a is a flowchart of the first part of the second sequencing process according to an embodiment of the present disclosure, and FIG5b is a flowchart of the second part of the second sequencing process according to an embodiment of the present disclosure. As shown in FIG5a and FIG5b, the sequencing process of simultaneously sequencing two slides (taking the sequencing of sequencing slide A203 and sequencing slide B205 as an example, wherein the sequencing process of sequencing slide A in the second sequencing process corresponds to FIG5a, and the sequencing process of sequencing slide B in the second sequencing process corresponds to FIG5b) includes: a sequencing preparation stage S2001, biochemical reaction stages S2002 and S2003, signal acquisition stages S2004 and S2005, and sequencing result generation stages S2006 and S2007. Specifically:

[0057] Step S2001: The user fills in the sequencing information. After the computer 207 receives the sequencing information filled in by the target subject, it first controls the lifting screen 102 to rise according to the sequencing information. After the user places sequencing reagent tank A and sequencing reagent tank B in the reagent insertion interface A107 and the reagent insertion interface B108, the lifting screen 102 descends and preloads the reagents. Then the lifting screen 102 rises, and the user places sequencing slide A203 and sequencing slide B205 in the sample insertion interface A105 and the sample insertion interface B106. In step S2001, the sequencing slide A or sequencing slide B corresponds to the first sequencing slide, thereby completing the sequencing preparation and entering the biochemical reaction stage of step S2002 and step S2003. Step S2002 corresponds to the biochemical reaction stage of sequencing slide A, and step S2003 corresponds to the biochemical reaction stage of sequencing slide B.

[0058] Step S2002: The computer 207 issues a fourth instruction to the fluidic device A via the control device 208. After receiving the fourth instruction, the fluidic device A pumps the reagents required for the biochemical reaction from the sequencing reagent tank A into the sequencing slide A 203 via the internal flow channel, so that the sample to be tested in the sequencing slide A undergoes a biochemical reaction and enters the current gene sequencing cycle. Within a preset time (e.g., 10 seconds) before the biochemical reaction is completed, the computer 207 detects whether the XY platform 201 is occupied by another sequencing slide (e.g., sequencing slide B). If occupied, the computer 207 waits until the XY platform 201 is released, and then the sequencing slide A 203 occupies the XY platform 201. The computer 207 issues a fifth instruction to the XY platform 201 via the control device 208. After receiving the fifth instruction, the XY platform 201 moves to the second position in the xy coordinate system. For example, the control device 208 moves the XY platform 201 to a specific coordinate position (XY) in the xy coordinate system. A1 , Y A1 ) so that the area on the sequencing slide A203 where signal detection is required is directly below the objective lens in the Z direction, and the signal acquisition stage of step S2004 is entered.

[0059] Step S2004: After the biochemical reaction is completed and the XY platform 201 moves to the second position, the computer 207 sends a sixth instruction to the data acquisition module 206 through the control device 208, and moves the objective lens in the Z direction via the Z axis to achieve focusing and signal acquisition on the sequencing slide A203.

[0060] It should be noted that one biochemical reaction corresponds to one cycle, and multiple signal acquisitions may be included in the cycle. Specifically, in the second sequencing process, when the sequencing slide A203 may contain one, two, or more areas that need to be detected (i.e., the second target area mentioned above), multiple signal acquisitions need to be performed in the cycle corresponding to this biochemical reaction. After each signal acquisition in the signal acquisition stage, it is necessary to determine whether the signal acquisition of all areas in the current cycle has been completed. If the signal acquisition of all areas in the current cycle has not been completed, the computer 207 moves the XY platform 201 to the next specific coordinate position (X A* , Y A* ), so that the next area of ​​the sequencing slide A203 that needs to be signal detected is directly below the objective lens in the Z direction, and the control device 208 issues a sixth instruction to the data acquisition module 206, so that the signal acquisition of the next area of ​​the sequencing slide A203 that needs to be signal acquired is realized through the sixth instruction, and the cycle is completed after all areas that need to be signal acquired in the sequencing slide A203 have completed signal acquisition; and after completing the signal acquisition of all areas in the current cycle (or this cycle), the XY platform 201 is released, and the process goes to step S2006.

[0061] Step S2006: Gene sequencing of the sample is achieved through a cycle of biochemical reaction S2002 and signal acquisition stage S2004, generating a sequencing result for sequencing slide A203. Specifically, since multiple biochemical reactions are required during the entire gene sequencing process, each biochemical reaction corresponds to a cycle. After signal acquisition is completed in all regions of the current cycle, XY platform 201 is released and a determination is made as to whether all gene sequencing cycles have been completed. The gene sequencing cycle includes steps S2002 and S2004. If all gene test cycles have not been completed, the process returns to step S2002 to continue biochemical reactions on the sample to be tested on sequencing slide A203. If all gene sequencing cycles have been completed, a sequencing result for sequencing slide A203 is generated.

[0062] After executing step S2001, the biochemical reaction corresponding to sequencing slide B proceeds to step S2003: the computer 207 issues a fourth instruction to the fluidic device B via the control device 208. After receiving the fourth instruction, the fluidic device B pumps the reagents required for the biochemical reaction from the sequencing reagent tank B into the sequencing slide B 205 via the internal flow channel, so that the sample to be tested in the sequencing slide B undergoes a biochemical reaction and enters the current gene sequencing cycle. Within a preset time (e.g., 10 seconds) before the completion of the biochemical reaction, the computer 207 detects whether the XY platform 201 is occupied by another sequencing slide (e.g., sequencing slide A). If occupied, the computer 207 waits until the XY platform 201 is released, and then the sequencing slide B 205 occupies the XY platform 201. The computer 207 issues a fifth instruction to the XY platform 201 via the control device 208. After receiving the fifth instruction, the XY platform 201 moves to the second position in the xy coordinate system, for example, moving the XY platform 201 to a specific coordinate position (XY) in the xy coordinate system. B1 , Y B1 ) so that the area on the sequencing slide B205 where signal detection is required is directly below the objective lens in the Z direction, and the signal acquisition stage of step S2005 is entered.

[0063] Step S2005: After the biochemical reaction is completed and the XY platform 201 moves to the second position, the computer 207 sends a sixth instruction to the data acquisition module 206 through the control device 208, and moves the objective lens in the Z direction via the Z axis to achieve focusing and signal acquisition on the sequencing slide B205.

[0064] It should be noted that one biochemical reaction corresponds to one cycle, and multiple signal acquisitions may be performed in the cycle. Specifically, when the sequencing slide B205 may contain one, two, or more regions that require signal detection (i.e., the second target region), multiple signal acquisitions are required in the cycle corresponding to this biochemical reaction. After each signal acquisition in the signal acquisition phase, it is necessary to determine whether signal acquisition of all regions in the current cycle has been completed. If signal acquisition of all regions in the current cycle has not been completed, the computer 207 moves the XY platform 201 to the next specific coordinate position (XY coordinate position) through the control device 208. B* , Y B* ), so that the next area of ​​the sequencing slide B205 that needs to be signal detected is directly below the objective lens in the Z direction, and the control device 208 issues a sixth instruction to the data acquisition module 206, so that the signal acquisition of the next area of ​​the sequencing slide B205 that needs to be signal acquired is realized through the sixth instruction, and the cycle is completed after all areas that need to be signal acquired in the sequencing slide B205 have completed signal acquisition; and after completing the signal acquisition of all areas in the current cycle (or this cycle), the XY platform 201 is released, and the process goes to step S2007.

[0065] Step S2007: Gene sequencing of the sample is achieved through a cycle of biochemical reaction S2003 and signal acquisition stage S2005, generating a sequencing result for sequencing slide B205. Specifically, since multiple biochemical reactions are required during the entire gene sequencing process, each biochemical reaction corresponds to one cycle. After signal acquisition is completed in all regions of the current cycle, XY platform 201 is released and a determination is made as to whether all cycles of the gene sequencing cycle have been completed. The gene sequencing cycle includes steps S2003 and S2005. If all cycles of the gene testing cycle have not been completed, the process returns to step S2003 to continue biochemical reactions on the sample to be tested on sequencing slide B205. If all cycles of the gene sequencing cycle have been completed, a sequencing result for sequencing slide B205 is generated.

[0066] During the second sequencing process, sequencing slide A (Figure 5a) and sequencing slide B (Figure 5b) alternately cycle through the XY platform, occupies and releases the platform, performing their respective biochemical reactions and signal acquisition, ultimately obtaining their respective sequencing results, and completing the co-sequencing of sequencing slide A 203 and sequencing slide B 205. By alternating the use of the two slides for sequencing, the instrument's operating time is maximized, idle time during sequencing is reduced, and sequencing efficiency is improved. Furthermore, because both slides can simultaneously perform biochemical reactions and signal acquisition, co-sequencing can shorten sequencing cycles and accelerate the production of sequencing results.

[0067] In another optional embodiment, the lifting screen 102 can be divided into independent lifting screen A and lifting screen B, and the XY platform 201 can be divided into independent XY platform A and XY platform B. Therefore, when sequencing together, sequencing carrier A and sequencing carrier B do not need to check the occupancy status of the XY platform, and there is no need to wait, which makes the use more flexible.

[0068] Optionally, the third sequencing process is to create a new process for performing gene sequencing on the third sequencing slide during any stage of performing gene sequencing on the second sequencing slide in the sequencing slide, wherein the stages of gene sequencing include a sequencing preparation stage, a biochemical reaction stage, a signal acquisition stage, and a sequencing result generation stage.

[0069] Optionally, the gene sequencing process corresponding to the second sequencing carrier includes: step S31, the computer receives sequencing information filled in by the target object, and controls the lifting and lowering of the lifting screen according to the sequencing information so that the target object is placed in the sequencing reagent tank and the second sequencing carrier in the gene sequencing system; step S32, the computer sends a seventh instruction to the fluid device in the first target biochemical reaction and detection platform where the second sequencing carrier is located through the control device, wherein the seventh instruction is used to control the fluid device to pump the reagents required for the biochemical reaction from the sequencing reagent tank into the second sequencing carrier so that the sample to be tested undergoes a biochemical reaction and enters the gene sequencing cycle of the current cycle, wherein the first target biochemical reaction and detection platform is the first biochemical reaction and detection platform or the second biochemical reaction and detection platform. Any one; within a preset time before the completion of the biochemical reaction, the computer detects the occupancy status of the XY platform in the gene sequencing system; when the XY platform is occupied by other sequencing carriers, after waiting for the XY platform to be released, the second sequencing carrier occupies the XY platform, and the computer sends an eighth instruction to the XY platform in the gene sequencing system through the control device, wherein the eighth instruction is used to control the XY platform to move to a third position so that the area of ​​the second sequencing carrier requiring signal detection is directly below the objective lens in the Z direction, and the objective lens is in the data acquisition module; step S33, after the biochemical reaction is completed, the computer sends a ninth instruction to the data acquisition module through the control device, wherein the ninth instruction is used to achieve focusing and signal acquisition on the second sequencing carrier.

[0070] Optionally, the gene sequencing process corresponding to the second sequencing slide also includes: when there are multiple areas in the second sequencing slide that need to perform signal acquisition, the computer controls the XY platform to move successively through the eighth instruction so that the third target area is located directly below the objective lens in the Z direction, wherein the third target area is a plurality of areas in the second sequencing slide that need to perform signal acquisition.

[0071] Optionally, the gene sequencing process corresponding to the second sequencing carrier also includes: after completing signal acquisition for all areas requiring signal acquisition in the second sequencing carrier, releasing the XY platform, and determining whether all cycles of the gene sequencing cycle are completed, wherein the gene sequencing cycle includes step S32 and step S33; if all cycles of the gene sequencing cycle are not completed, returning to step S32 to continue gene sequencing of the sample to be tested in the second sequencing carrier, and if all cycles of the gene sequencing cycle are completed, executing step S34; step S34, generating the sequencing result of the second sequencing carrier.

[0072] Optionally, the gene sequencing process corresponding to the third sequencing carrier includes: step S41, receiving sequencing information filled in by the target object and detecting the occupancy status of the XY platform in the gene sequencing system; when the XY platform is occupied by other sequencing carriers, waiting for the XY platform to be released, the third sequencing carrier occupies the XY platform, controlling the lifting and lowering of the lifting screen according to the sequencing information to allow the target object to place the sequencing reagent tank and sequencing carrier in the gene sequencing system, and releasing the XY platform after the sequencing reagent tank and sequencing carrier are placed in the gene sequencing system; step S42, the computer sends a tenth instruction to the fluid device in the second target biochemical reaction and detection platform where the third sequencing carrier is located through the control device, wherein the tenth instruction is used to control the fluid device to pump reagents required for the biochemical reaction from the sequencing reagent tank into the third sequencing carrier so that the sample to be tested undergoes a biochemical reaction. And enter the gene sequencing cycle of the current period, wherein the second target biochemical reaction and detection platform is any one of the first biochemical reaction and detection platform or the second biochemical reaction and detection platform; within a preset time before the biochemical reaction is completed, the computer detects the occupancy status of the XY platform in the gene sequencing system; when the XY platform is occupied by other sequencing carriers, after waiting for the XY platform to be released, the third sequencing carrier occupies the XY platform, and the computer sends an eleventh instruction to the XY platform in the gene sequencing system through the control device, wherein the eleventh instruction is used to control the XY platform to move to the fourth position so that the area of ​​the third sequencing carrier requiring signal detection is directly below the objective lens in the Z direction, and the objective lens is in the data acquisition module; step S43, after the biochemical reaction is completed, the computer sends a twelfth instruction to the data acquisition module through the control device, wherein the twelfth instruction is used to achieve focusing and signal acquisition on the third sequencing carrier.

[0073] Optionally, the gene sequencing process corresponding to the third sequencing slide also includes: when there are multiple areas in the third sequencing slide that need to perform signal acquisition, the computer controls the XY platform to move successively through the eleventh instruction so that the fourth target area is located directly below the objective lens in the Z direction, wherein the fourth target area is a plurality of areas in the third sequencing slide that need to perform signal acquisition.

[0074] Optionally, the gene sequencing process corresponding to the third sequencing carrier also includes: after completing signal acquisition for all areas requiring signal acquisition in the third sequencing carrier, releasing the XY platform, and determining whether all cycles of the gene sequencing cycle are completed, wherein the gene sequencing cycle includes step S42 and step S43; if all cycles of the gene sequencing cycle are not completed, returning to step S42 to continue gene sequencing of the sample to be tested in the third sequencing carrier, and if all cycles of the gene sequencing cycle are completed, executing step S44; step S44, generating the sequencing result of the third sequencing carrier.

[0075] In some embodiments of the present disclosure, the third sequencing process is reflected in that during the sequencing process of a sequencing slide being sequenced by the sequencing system, new sequencing is performed on another sequencing slide, that is, in any stage of gene sequencing on the second sequencing slide among the sequencing slides, a new process of gene sequencing on the third sequencing slide is created, wherein the stages of gene sequencing include a sequencing preparation stage, a biochemical reaction stage, a signal acquisition stage, and a sequencing result generation stage.

[0076] The third sequencing process is explained below with reference to FIG6a and FIG6b. FIG6a is a flowchart of the first part of the third sequencing process according to an embodiment of the present disclosure, and FIG6b is a flowchart of the second part of the third sequencing process according to an embodiment of the present disclosure. As shown in FIG6a and FIG6b, the sequencing process of rolling sequencing of two slides (taking rolling sequencing of sequencing slide A203 and sequencing slide B205 as an example, wherein the sequencing process of sequencing slide A in the third sequencing process corresponds to FIG6a, and the sequencing process of sequencing slide B in the third sequencing process corresponds to FIG6b) includes: sequencing preparation stages S3001 and S3005, biochemical reaction stages S3002 and S3006, signal acquisition stages S3003 and S3007, and sequencing result generation stages S3004 and S3008. Specifically:

[0077] The gene sequencing process corresponding to sequencing slide A203 is shown in Figure 6a and is as follows:

[0078] Step S3001: The user enters sequencing information. After receiving the sequencing information entered by the target subject, the computer 207 first controls the lifting screen 102 to rise according to the sequencing information. After the user places the sequencing reagent tank A on the reagent insertion interface A107, the lifting screen 102 descends and preloads the reagent. Then, the lifting screen 102 rises, and the user places the sequencing slide A203 on the sample insertion interface A105. In step S3001, the sequencing slide A203 corresponds to the second sequencing slide mentioned above, thereby completing the sequencing preparation and entering the biochemical reaction stage of step S3002.

[0079] Step S3002: The computer 207 issues a seventh instruction to the fluidic device A via the control device 208. After receiving the seventh instruction, the fluidic device A pumps the reagents required for the biochemical reaction from the sequencing reagent tank A into the sequencing slide A 203 via the internal flow channel, causing the sample to be tested in the sequencing slide A to undergo a biochemical reaction and enter the current gene sequencing cycle. Within a preset time (e.g., 10 seconds) before the biochemical reaction on the sequencing slide A 203 is completed, the computer 207 detects whether the XY platform 201 is occupied by another sequencing slide. If occupied, the computer 207 waits until the XY platform 201 is released, and then the sequencing slide A 203 occupies the XY platform 201. The computer 207 issues an eighth instruction to the XY platform 201 via the control device 208. After receiving the eighth instruction, the XY platform 201 moves to a third position in the xy coordinate system. For example, the control device 208 moves the XY platform 201 to a specific coordinate position (XY) in the xy coordinate system. A1 , Y A1 ) so that the area on the sequencing slide A203 where signal detection is required is directly below the objective lens in the Z direction, and the signal acquisition stage of step S3003 is entered.

[0080] Step S3003: After the biochemical reaction is completed and the XY platform 201 moves to the third position, the computer 207 sends a ninth instruction to the data acquisition module 206 through the control device 208, and moves the objective lens in the Z direction via the Z axis to achieve focusing and signal acquisition on the sequencing slide A203.

[0081] It should be noted that one biochemical reaction corresponds to one cycle, and multiple signal acquisitions may be included in the cycle. Specifically, in the third sequencing process, when the sequencing slide A203 may contain one, two, or more areas that need to be detected (i.e., the third target area), multiple signal acquisitions are required in the cycle corresponding to this biochemical reaction. After each signal acquisition in the signal acquisition phase, it is necessary to determine whether the signal acquisition of all areas in the current cycle has been completed. If the signal acquisition of all areas in the current cycle has not been completed, the computer 207 moves the XY platform 201 to a specific coordinate position (X A* , Y A* ), so that the next area of ​​the sequencing slide A203 that needs to be detected by signal is directly below the objective lens in the Z direction, and the control device 208 sends a ninth instruction to the data acquisition module 206, so that the signal acquisition of the next area of ​​the sequencing slide A203 that needs to be detected by signal is realized through the ninth instruction, and the cycle is completed after all the areas that need to be detected by signal in the sequencing slide A203 have completed signal acquisition; and after completing the signal acquisition of all areas in the current cycle (or this cycle), the XY platform 201 is released, and the process goes to step S3004.

[0082] Step S3004: Gene sequencing of the sample is achieved through a cycle of biochemical reaction S3002 and signal acquisition S3003, generating a sequencing result for sequencing slide A203. Specifically, since multiple biochemical reactions are required during the entire gene sequencing process, each biochemical reaction corresponds to one cycle. After signal acquisition is completed for all regions of the current cycle, XY platform 201 is released, and a determination is made as to whether all gene sequencing cycles have been completed. The gene sequencing cycle includes steps S3002 and S3003. If all gene test cycles have not been completed, the process returns to step S3002 to continue biochemical reactions on the sample to be tested on sequencing slide A203. If all gene sequencing cycles have been completed, a sequencing result for sequencing slide A203 is generated.

[0083] When the sequencing slide A203 is in any of the stages S3001, S3002, S3003, and S3004, a new sequencing can be performed on the sequencing slide B205. The sequencing slide B205 is the third sequencing slide mentioned above, as shown in FIG6 b . Specifically, the steps include:

[0084] Step S3005: The user fills in the sequencing information. After receiving the sequencing information filled in by the target subject, the computer 207 checks whether the XY platform 201 is occupied by other sequencing carriers (such as sequencing carrier A). If occupied, the computer 207 waits until the XY platform 201 is released, and then sequencing carrier B 205 occupies the XY platform 201. After the lifting screen 102 rises, the user places sequencing reagent tank B on the reagent insertion interface B108. Then the lifting screen 102 descends and preloads the reagents. The computer 207 checks again whether the XY platform 201 is occupied by other sequencing carriers (such as sequencing carrier A). If occupied, the computer 207 waits until the XY platform 201 is released, and then sequencing carrier B 205 occupies the XY platform 201. Then the lifting screen 102 rises. After the user places sequencing carrier B 205 on the sample insertion interface B106, the lifting screen 102 descends and releases the XY platform 201, thereby completing the sequencing preparation and entering the biochemical reaction stage of step S3006.

[0085] Step S3006: The computer 207 issues a tenth instruction to the fluidic device B via the control device 208. After receiving the tenth instruction, the fluidic device B pumps the reagents required for the biochemical reaction from the sequencing reagent tank B into the sequencing slide B 205 through the internal flow channel, so that the sample to be tested on the sequencing slide B undergoes a biochemical reaction and enters the current gene sequencing cycle. Within a preset time (e.g., 10 seconds) before the biochemical reaction on the sequencing slide B 205 is completed, the computer 207 detects whether the XY platform 201 is occupied by another sequencing slide (e.g., sequencing slide A). If occupied, the computer 207 waits until the XY platform 201 is released, and then the sequencing slide B 205 occupies the XY platform 201. The computer 207 issues an eleventh instruction to the XY platform 201 via the control device 208. After receiving the eleventh instruction, the XY platform 201 moves to the fourth position in the xy coordinate system. For example, the control device 208 moves the XY platform 201 to a specific coordinate position (XY) in the xy coordinate system. B1 , Y B1 ) so that the area on the sequencing slide B205 where signal detection is required is directly below the objective lens in the Z direction, and the signal acquisition stage of step S3007 is entered.

[0086] Step S3007: After the biochemical reaction is completed and the XY platform 201 moves to the fourth position, the computer 207 sends the twelfth instruction to the data acquisition module 206 through the control device 208, and moves the objective lens in the Z direction through the Z axis to achieve focusing and signal acquisition on the sequencing carrier B205.

[0087] It should be noted that one biochemical reaction corresponds to one cycle, and multiple signal acquisitions may be included in the cycle. Specifically, in the third sequencing process, when the sequencing slide B205 may contain one, two, or more areas that need to be detected (i.e., the fourth target area), multiple signal acquisitions are required in the cycle corresponding to this biochemical reaction. After each signal acquisition in the signal acquisition stage, it is necessary to determine whether the signal acquisition of all areas in the current cycle has been completed. If the signal acquisition of all areas in the current cycle has not been completed, the computer 207 moves the XY platform 201 to a specific coordinate position (X B* , Y B* ), so that the next area of ​​the sequencing slide B205 that needs to be detected by signal is directly below the objective lens in the Z direction, and the control device 208 sends a twelfth instruction to the data acquisition module 206, so that the signal acquisition of the next area of ​​the sequencing slide B205 that needs to be detected by signal is realized through the twelfth instruction, and the signal acquisition of the next area of ​​the sequencing slide B205 that needs to be detected by signal is completed until all areas of the sequencing slide B205 that need to be detected by signal have completed signal acquisition, and this cycle is completed; and after completing the signal acquisition of all areas in the current cycle (or this cycle), the XY platform 201 is released, and the process goes to step S3008.

[0088] Step S3008: Gene sequencing of the sample is achieved through a cycle of biochemical reaction S3006 and signal acquisition S3007, generating a sequencing result for sequencing slide B205. Specifically, since multiple biochemical reactions are required during the entire gene sequencing process, each biochemical reaction corresponds to one cycle. After signal acquisition is completed for all regions of the current cycle, XY platform 201 is released, and a determination is made as to whether all gene sequencing cycles have been completed. The gene sequencing cycle includes steps S3006 and S3007. If all gene test cycles have not been completed, the process returns to step S3006 to continue biochemical reactions on the sample to be tested on sequencing slide B205. If all gene sequencing cycles have been completed, a sequencing result for sequencing slide B205 is generated.

[0089] In another optional embodiment, the lifting screen 102 can be divided into independent lifting screen A and lifting screen B, and the XY platform 201 can be divided into independent XY platform A and XY platform B. Therefore, during rolling sequencing, the sequencing carrier A and the sequencing carrier B do not need to check the occupancy of the XY platform, and there is no need to wait, which makes the use more flexible.

[0090] In the above-mentioned third sequencing process, at any stage of gene sequencing on the second sequencing carrier in the sequencing carrier, a new process for gene sequencing on the third sequencing carrier can be created. At the same time, the second sequencing carrier and the third sequencing carrier sequentially perform a cyclic operation of occupying and releasing the XY platform, which can realize their respective biochemical reactions and signal acquisition, and ultimately obtain their respective sequencing results, thereby reducing the waiting time of the third sequencing carrier and manual intervention in the operation, and improving the efficiency of the gene sequencing system; in addition, the rolling sequencing of the two carriers also reduces the idle time of the gene sequencing system and improves the utilization rate of the gene sequencing equipment.

[0091] In the gene sequencing system provided in the embodiments of the present disclosure, a compact optimized design enables biochemical reactions and signal detection to be performed on sequencing slides on the same platform. Compared with related technologies, signal detection can be performed immediately after the biochemical reaction is completed, eliminating the need for a transfer step between the biochemical reaction platform and the signal acquisition platform. This speeds up the overall sequencing time. At the same time, the required internal space for movement is small, reducing the overall cost and size of the sequencing system. In addition, the flexibility of the gene sequencing system in performing gene sequencing on multiple sequencing slides can be enhanced.

[0092] FIG7 is a flow chart of a gene sequencing method according to an embodiment of the present disclosure. As shown in FIG7 , the method includes the following steps:

[0093] Step S602, obtaining the number of sequencing slides to be sequenced;

[0094] Step S604: Determine a sequencing process based on the number of sequencing slides, wherein the sequencing process is used to control the first biochemical reaction and detection platform and the second biochemical reaction and detection platform to perform synchronous or asynchronous sequencing processes, respectively, by a control device in the gene sequencing system. The gene sequencing system includes the control device, the first biochemical reaction and detection platform, and the second biochemical reaction and detection platform.

[0095] Step S606, when the number of sequencing slides is 1, execute the first sequencing process; when the number of sequencing slides is greater than 1 and the sequencing slides are sequenced simultaneously, execute the second sequencing process; when the number of sequencing slides is greater than 1 and the sequencing slides are not sequenced simultaneously, execute the third sequencing process.

[0096] Through the above steps S602 to S606, the purpose of performing biochemical reactions and information detection on the sequencing slide on the same platform can be achieved, and there is no need to transfer the sequencing slide between the biochemical reaction platform and the signal detection platform, thereby speeding up the overall sequencing time, reducing the overall cost and size of the gene sequencing system, and solving the technical problems of large gene sequencing system size and long sequencing time caused by the gene sequencing system in the related art requiring two platforms to perform biochemical reactions and signal detection on the sequencing slide respectively when performing gene sequencing. In addition, the above steps can also realize the sequencing of a single slide (i.e., the first sequencing process described above), the simultaneous sequencing of two slides (i.e., the second sequencing process described above), and the rolling sequencing of two slides (i.e., the third sequencing process described above), thereby enhancing the flexibility of the gene sequencing system.

[0097] It should be noted that the gene sequencing method shown in Figure 7 can be applied to the gene sequencing system shown in Figure 2. Therefore, the relevant explanations in the above gene sequencing system are also applicable to the gene sequencing method and will not be repeated here.

[0098] The gene sequencing method embodiment provided in the embodiments of the present disclosure can be executed in a mobile terminal, a computer terminal or a similar computing device. Figure 8 shows a hardware structure block diagram of a computer terminal for implementing the gene sequencing method. As shown in Figure 8, the computer terminal 70 may include one or more (702a, 702b, ..., 702n are used in the figure to illustrate) processors (the processor may include but is not limited to a processing device such as a microprocessor MCU or a programmable logic device FPGA), a memory 704 for storing data, and a transmission module 706 for communication functions connected via a wired and / or wireless network. In addition, it may also include: a display, a keyboard, a cursor control device, an input / output interface (I / O interface), a universal serial bus (USB) port (which may be included as one of the ports of the I / O interface), a network interface, and a BUS bus. It will be understood by those skilled in the art that the structure shown in Figure 8 is only illustrative and does not limit the structure of the above-mentioned electronic device. For example, the computer terminal 70 may also include more or fewer components than those shown in Figure 8, or have a configuration different from that shown in Figure 8.

[0099] It should be noted that the one or more processors and / or other data processing circuits described above may generally be referred to herein as "data processing circuitry." The data processing circuitry may be embodied in whole or in part as software, hardware, firmware, or any other combination thereof. Furthermore, the data processing circuitry may be a single, independent processing module, or may be fully or partially integrated into any of the other components of the computer terminal 70. As described in the embodiments of the present disclosure, the data processing circuitry serves as a processor control (e.g., selection of a variable resistor terminal path connected to an interface).

[0100] The memory 704 can be used to store software programs and modules of application software, such as the program instructions / data storage device corresponding to the gene sequencing method in the embodiment of the present disclosure. The processor executes various functional applications and data processing by running the software programs and modules stored in the memory 704, that is, implementing the above-mentioned gene sequencing method. The memory 704 may include a high-speed random access memory, and may also include a non-volatile memory, such as one or more magnetic storage devices, flash memory, or other non-volatile solid-state memory. In some instances, the memory 704 may further include a memory remotely located relative to the processor, and these remote memories may be connected to the computer terminal 70 via a network. Examples of the above-mentioned network include, but are not limited to, the Internet, an intranet, a local area network, a mobile communication network, and combinations thereof.

[0101] The transmission module 706 is configured to receive or transmit data via a network. A specific example of the aforementioned network may include a wireless network provided by the communications provider of the computer terminal 70. In one embodiment, the transmission module 706 includes a network interface controller (NIC), which can be connected to other network devices via a base station to enable communication with the Internet. In another embodiment, the transmission module 706 may be a radio frequency (RF) module, which is configured to communicate with the Internet wirelessly.

[0102] The display may be, for example, a touch screen liquid crystal display (LCD) that enables a user to interact with a user interface of the computer terminal 70 .

[0103] It should be noted that, in some optional embodiments, the computer terminal shown in FIG8 may include hardware components (including circuits), software components (including computer code stored on a computer-readable medium), or a combination of hardware components and software components. It should be noted that FIG8 is only an example of a specific embodiment and is intended to illustrate the types of components that may be present in the computer terminal.

[0104] An embodiment of the present disclosure also provides a gene sequencing electronic device, comprising: a memory for storing program instructions; and a processor connected to the memory for executing the above-mentioned gene sequencing method.

[0105] An embodiment of the present disclosure further provides a non-volatile storage medium, wherein the non-volatile storage medium includes a stored computer program, wherein the device where the non-volatile storage medium is located executes the above-mentioned gene sequencing method by running the computer program.

[0106] The embodiments of the present disclosure also provide a computer program product, including a non-volatile computer-readable storage medium, which stores a computer program. When the computer program is executed by a processor, it implements the steps of the gene sequencing method in each embodiment of the present application.

[0107] An embodiment of the present application also provides a computer program product, including computer instructions, which, when executed by a processor, implement the steps of the gene sequencing method in each embodiment of the present application.

[0108] The embodiments of the present application also provide a computer program, which, when executed by a processor, implements the steps of the gene sequencing method in each embodiment of the present application.

[0109] The serial numbers of the above-mentioned embodiments of the present disclosure are for description only and do not represent the advantages or disadvantages of the embodiments.

[0110] In the above embodiments of the present disclosure, the description of each embodiment has its own focus. For parts that are not described in detail in a certain embodiment, reference can be made to the relevant descriptions of other embodiments.

[0111] In the several embodiments provided in the present disclosure, it should be understood that the disclosed technical content can be implemented in other ways. Among them, the device embodiments described above are only exemplary. For example, the division of the units can be a logical function division. In actual implementation, there may be other division methods, such as multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. Another point is that the mutual coupling or direct coupling or communication connection shown or discussed can be through some interfaces, indirect coupling or communication connection of units or modules, which can be electrical or other forms.

[0112] The units described as separate components may or may not be physically separate, and the components shown as units may or may not be physical units, that is, they may be located in one place or distributed across multiple units. Some or all of the units may be selected according to actual needs to achieve the purpose of the present embodiment.

[0113] In addition, the functional units in the various embodiments of the present disclosure may be integrated into a single processing unit, or each unit may exist physically separately, or two or more units may be integrated into a single unit. The aforementioned integrated units may be implemented in the form of hardware or software functional units.

[0114] If the integrated unit is implemented in the form of a software functional unit and sold or used as an independent product, it can be stored in a computer-readable storage medium. Based on this understanding, the technical solution of the present invention is essentially or the part that contributes to the prior art or all or part of the technical solution can be embodied in the form of a software product, and the computer software product is stored in a storage medium, including a number of instructions for enabling a computer device (which can be a personal computer, a server or a network device, etc.) to perform all or part of the steps of the method described in each embodiment of the present invention. The aforementioned storage medium includes: various media that can store program codes, such as a USB flash drive, a read-only memory (ROM), a random access memory (RAM), a mobile hard disk, a magnetic disk or an optical disk.

[0115] The above is only a preferred embodiment of the present disclosure. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present disclosure. These improvements and modifications should also be regarded as within the scope of protection of the present disclosure.

Claims

1. A gene sequencing system, characterized in that: include: control device, a first biochemical reaction and detection platform and a second biochemical reaction and detection platform, wherein: The control device is used to control the first biochemical reaction and detection platform and the second biochemical reaction and detection platform to respectively perform synchronous or asynchronous sequencing processes.

2. The gene sequencing system according to claim 1, characterized in that Also includes: Data acquisition module, where The data acquisition module is used to collect data on the sequencing slide after the biochemical reaction on the first biochemical reaction and detection platform or the second biochemical reaction and detection platform is moved to the sampling position by the control device.

3. The gene sequencing system according to claim 2, characterized in that: The gene sequencing system further includes an XY platform, on which the first biochemical reaction and detection platform and the second biochemical reaction and detection platform are arranged, wherein the XY platform is used to move within a preset range of an XY coordinate system.

4. The gene sequencing system according to claim 1, wherein: The first biochemical reaction and detection platform and the second biochemical reaction and detection platform include a fluid device and a fixing device, wherein the fluid device is used to pump reagents required for biochemical reactions from a sequencing reagent tank into a sequencing slide, and the fixing device is used to fix the sequencing slide placed on the biochemical reaction and detection platform. The fluid device, the fixing device and the sequencing slide are connected by an internal flow channel.

5. The gene sequencing system according to claim 3, characterized in that: The gene sequencing system further includes a computer, which is connected to the control device and the data acquisition module respectively, and is used to send control instructions to the control device and obtain data information from the control device.

6. The gene sequencing system according to claim 5, characterized in that: The control device is used to receive the control instruction, control the data acquisition module according to the control instruction, and control the fluid device and the fixing device in the first biochemical reaction and detection platform and / or the second biochemical reaction and detection platform.

7. The gene sequencing system according to claim 5, characterized in that: The computer is also used to obtain the number of sequencing slides to be sequenced; determine the sequencing process based on the number of sequencing slides, wherein when the number of sequencing slides is 1, it corresponds to the first sequencing process; when the number of sequencing slides is greater than 1 and the sequencing slides are sequenced simultaneously, it corresponds to the second sequencing process; when the number of sequencing slides is greater than 1 and the sequencing slides are not sequenced simultaneously, it corresponds to the third sequencing process.

8. The gene sequencing system according to claim 7, characterized in that: The first sequencing process includes: Step S11: The computer receives the sequencing information of the target object and controls the Controlling the lifting of the lifting screen so that the target object can be placed in the sequencing reagent tank and sequencing slide in the gene sequencing system; Step S12: The computer sends a first instruction to the fluid device in the first biochemical reaction and detection platform or the second biochemical reaction and detection platform via the control device, wherein the first instruction is used to control the fluid device to pump reagents required for the biochemical reaction from the sequencing reagent tank into the sequencing slide, so that the sample to be tested undergoes a biochemical reaction and enters the current gene sequencing cycle; Within a preset time before the biochemical reaction is completed, the computer sends a second instruction to the XY stage in the gene sequencing system through the control device, wherein the second instruction is used to control the XY stage to move to a first position so that the area of ​​the sequencing slide where signal detection is required is directly below the objective lens in the Z direction, and the objective lens is in the data acquisition module; Step S13: After the biochemical reaction is completed, the computer sends a third instruction to the data acquisition module through the control device, wherein the third instruction is used to achieve focusing and signal acquisition on the sequencing slide.

9. The gene sequencing system according to claim 8, characterized in that: When there are multiple areas in the sequencing slide that require signal acquisition, the computer controls the XY platform to move successively through the second instruction so that the first target area is located directly below the objective lens in the Z direction, wherein the first target area is the multiple areas in the sequencing slide that require signal acquisition.

10. The gene sequencing system according to claim 8, characterized in that: The first sequencing process also includes: After completing signal acquisition for all regions requiring signal acquisition in the sequencing slide, determining whether all cycles of gene sequencing cycles are completed, wherein the gene sequencing cycles include step S12 and step S13; If all the gene sequencing cycles are not completed, return to step S12 to continue gene sequencing of the sample to be tested. If all the gene sequencing cycles are completed, execute step S14. Step S14: generating the sequencing results of the sequencing slide.

11. The gene sequencing system according to claim 7, characterized in that: The second sequencing process includes: Step S21, the computer receives sequencing information entered by the target subject, and controls the lifting and lowering of the lifting screen according to the sequencing information so that the target subject can place the sequencing reagent tank and sequencing slide in the gene sequencing system; Step S22: the computer sends a fourth instruction to the first fluid device in the first biochemical reaction and detection platform and the second fluid device in the second biochemical reaction and detection platform through the control device. The fourth instruction is used to control the first fluidic device and the second fluidic device to pump the reagents required for the biochemical reaction from the sequencing reagent tank into the corresponding sequencing slide, so that the sample to be tested undergoes a biochemical reaction and enters the current gene sequencing cycle. Each fluidic device corresponds to one sequencing slide. The computer detects the occupancy status of the XY platform in the gene sequencing system within a preset time before the biochemical reaction is completed; In a state where the XY platform is occupied by other sequencing slides, after waiting for the XY platform to be released, a first sequencing slide among the sequencing slides occupies the XY platform, and the computer sends a fifth instruction to the XY platform in the gene sequencing system through the control device, wherein the fifth instruction is used to control the XY platform to move to a second position so that a region of the first sequencing slide requiring signal detection is directly below an objective lens in a Z direction, the objective lens being in the data acquisition module, and the first sequencing slide being any one of the sequencing slides corresponding to the first biochemical reaction and detection platform or the second biochemical reaction and detection platform; Step S23: After the biochemical reaction is completed, the computer sends a sixth instruction to the data acquisition module through the control device, wherein the sixth instruction is used to achieve focusing and signal acquisition on the first sequencing slide.

12. The gene sequencing system according to claim 11, characterized in that: When there are multiple areas in the first sequencing slide that require signal acquisition, the computer controls the XY platform to move successively through the fifth instruction so that the second target areas are located directly below the objective lens in the Z direction, wherein the second target areas are multiple areas in the first sequencing slide that require signal acquisition.

13. The gene sequencing system according to claim 12, characterized in that: The second sequencing process also includes: After completing signal acquisition for all regions requiring signal acquisition in the first sequencing slide, releasing the XY platform and determining whether all cycles of gene sequencing are completed, wherein the gene sequencing cycle includes step S22 and step S23; If all the gene sequencing cycles are not completed, return to step S22 to continue gene sequencing of the sample on the first sequencing slide. If all the gene sequencing cycles are completed, execute step S24. Step S24: Generate sequencing results of the first sequencing slide.

14. The gene sequencing system according to claim 7, characterized in that: The third sequencing process is a process for performing gene sequencing on a third sequencing slide in any stage of performing gene sequencing on the second sequencing slide in the sequencing slides, wherein the stages of gene sequencing include a sequencing preparation stage, a biochemical reaction stage, a signal acquisition stage, and a sequencing result generation stage.

15. The gene sequencing system according to claim 14, characterized in that: The gene sequencing process corresponding to the second sequencing slide includes: Step S31, the computer receives sequencing information entered by the target subject, and controls the lifting and lowering of the lifting screen according to the sequencing information so that the target subject is placed in the sequencing reagent tank and the second sequencing slide in the gene sequencing system; Step S32: The computer sends a seventh instruction via the control device to the fluidic device in the first target biochemical reaction and detection platform where the second sequencing slide is located, wherein the seventh instruction is used to control the fluidic device to pump reagents required for the biochemical reaction from the sequencing reagent reservoir into the second sequencing slide so that the sample to be tested undergoes a biochemical reaction and enters the current gene sequencing cycle, wherein the first target biochemical reaction and detection platform is either the first biochemical reaction and detection platform or the second biochemical reaction and detection platform. The computer detects the occupancy status of the XY platform in the gene sequencing system within a preset time before the biochemical reaction is completed; When the XY platform is occupied by another sequencing slide, after waiting for the XY platform to be released, the second sequencing slide occupies the XY platform, and the computer sends an eighth instruction to the XY platform in the gene sequencing system through the control device, wherein the eighth instruction is used to control the XY platform to move to a third position so that the area of ​​the second sequencing slide requiring signal detection is directly below the objective lens in the Z direction, and the objective lens is in the data acquisition module; Step S33: After the biochemical reaction is completed, the computer sends a ninth instruction to the data acquisition module through the control device, wherein the ninth instruction is used to achieve focusing and signal acquisition on the second sequencing slide.

16. The gene sequencing system according to claim 15, characterized in that: The gene sequencing process corresponding to the second sequencing slide also includes: when there are multiple areas in the second sequencing slide that require signal acquisition, the computer controls the XY platform to move successively through the eighth instruction so that the third target area is successively located directly below the objective lens in the Z direction, wherein the third target area is a plurality of areas in the second sequencing slide that require signal acquisition.

17. The gene sequencing system according to claim 15, characterized in that: The gene sequencing process corresponding to the second sequencing slide also includes: After completing signal acquisition for all regions requiring signal acquisition in the second sequencing slide, releasing the XY platform and determining whether all cycles of gene sequencing are completed, wherein the gene sequencing cycle includes step S32 and step S33; If all the gene sequencing cycles are not completed, return to step S32 to continue gene sequencing of the sample on the second sequencing slide. If all the gene sequencing cycles are completed, execute step S34. Step S34: Generate sequencing results for the second sequencing slide.

18. The gene sequencing system according to claim 14, characterized in that: The gene sequencing process corresponding to the third sequencing slide includes: Step S41, receiving sequencing information filled in by the target subject, and detecting the occupancy status of the XY platform in the gene sequencing system; When the XY platform is occupied by another sequencing slide, the third sequencing slide occupies the XY platform after waiting for the XY platform to be released, and the lifting and lowering of the lifting screen is controlled according to the sequencing information so that the target object places the sequencing reagent tank and sequencing slide in the gene sequencing system, and the XY platform is released after the sequencing reagent tank and sequencing slide are placed in the gene sequencing system; In step S42, the computer sends a tenth instruction via the control device to the fluid device in the second target biochemical reaction and detection platform where the third sequencing slide is located, wherein the tenth instruction is used to control the fluid device to pump reagents required for the biochemical reaction from the sequencing reagent tank into the third sequencing slide so that the sample to be tested undergoes a biochemical reaction and enters the current gene sequencing cycle, wherein the second target biochemical reaction and detection platform is either the first biochemical reaction and detection platform or the second biochemical reaction and detection platform. The computer detects the occupancy status of the XY platform in the gene sequencing system within a preset time before the biochemical reaction is completed; When the XY platform is occupied by another sequencing slide, the third sequencing slide occupies the XY platform after waiting for the XY platform to be released, and the computer sends an eleventh instruction to the XY platform in the gene sequencing system through the control device, wherein the eleventh instruction is used to control the XY platform to move to a fourth position so that the area of ​​the third sequencing slide requiring signal detection is directly below the objective lens in the Z direction, and the objective lens is in the data acquisition module; Step S43: After the biochemical reaction is completed, the computer sends a twelfth instruction to the data acquisition module through the control device, wherein the twelfth instruction is used to achieve focusing and signal acquisition on the third sequencing slide.

19. The gene sequencing system according to claim 18, characterized in that: The gene sequencing process corresponding to the third sequencing slide also includes: when there are multiple areas in the third sequencing slide that need to perform signal acquisition, the computer controls the XY platform to move successively through the eleventh instruction so that the fourth target area is located directly below the objective lens in the Z direction in sequence, wherein the fourth target area is a plurality of areas in the third sequencing slide that need to perform signal acquisition.

20. The gene sequencing system according to claim 18, characterized in that The gene sequencing process corresponding to the third sequencing slide also includes: After completing signal acquisition for all regions requiring signal acquisition in the third sequencing slide, releasing the XY platform and determining whether all cycles of gene sequencing are completed, wherein the gene sequencing cycle includes step S42 and step S43; If all the gene sequencing cycles are not completed, return to step S42 to continue gene sequencing the sample on the third sequencing slide. If all the gene sequencing cycles are completed, execute step S44. Step S44: Generate the sequencing results of the third sequencing slide.

21. A gene sequencing method, characterized in that: include: Obtaining the number of sequencing slides to be sequenced; Determining a sequencing process based on the number of sequencing slides, wherein the sequencing process is used to control a first biochemical reaction and detection platform and a second biochemical reaction and detection platform to respectively perform synchronous or asynchronous sequencing processes through a control device in a gene sequencing system, wherein the gene sequencing system includes the control device, the first biochemical reaction and detection platform, and the second biochemical reaction and detection platform; The first sequencing process is performed when the number of sequencing slides is 1, the second sequencing process is performed when the number of sequencing slides is greater than 1 and the sequencing slides are sequenced simultaneously, and the third sequencing process is performed when the number of sequencing slides is greater than 1 and the sequencing slides are not sequenced simultaneously.

22. The method according to claim 21, characterized in that The first sequencing process includes: Step S11, receiving sequencing information filled in by a target subject, and controlling the lifting and lowering of a lifting screen according to the sequencing information so that the target subject can place a sequencing reagent tank and a sequencing slide in the gene sequencing system; Step S12: sending a first instruction to the fluid device in the first biochemical reaction and detection platform or the second biochemical reaction and detection platform via the control device, wherein the first instruction is used to control the fluid device to pump reagents required for the biochemical reaction from the sequencing reagent tank into the sequencing slide, so that the sample to be tested undergoes a biochemical reaction and enters the current gene sequencing cycle; Within a preset time before the biochemical reaction is completed, the control device sends a second instruction to the XY platform in the gene sequencing system, wherein the second instruction is used to control the XY platform to move to the first position so that the area of ​​the sequencing slide where signal detection is required is directly below the objective lens in the Z direction. The objective lens is in a data acquisition module in the gene sequencing system; Step S13: After the biochemical reaction is completed, a third instruction is sent to the data acquisition module through the control device, wherein the third instruction is used to achieve focusing and signal acquisition on the sequencing slide.

23. The method according to claim 22, characterized in that When there are multiple areas in the sequencing slide that require signal collection, the second instruction controls the XY platform to move successively to positions where the first target areas are located directly below the objective lens in the Z direction, wherein the first target areas are multiple areas in the sequencing slide that require signal collection.

24. The method according to claim 22, characterized in that The first sequencing process also includes: After completing signal acquisition for all regions requiring signal acquisition in the sequencing slide, determining whether all cycles of gene sequencing cycles are completed, wherein the gene sequencing cycles include step S12 and step S13; If all the gene sequencing cycles are not completed, return to step S12 to continue gene sequencing of the sample to be tested. If all the gene sequencing cycles are completed, execute step S14. Step S14: generating the sequencing results of the sequencing slide.

25. The method according to claim 21, characterized in that The second sequencing process includes: Step S21, receiving sequencing information filled in by a target subject, and controlling the lifting and lowering of a lifting screen according to the sequencing information so that the target subject can place a sequencing reagent tank and a sequencing slide in the gene sequencing system; Step S22: Sending a fourth instruction via the control device to the first fluid device in the first biochemical reaction and detection platform and the second fluid device in the second biochemical reaction and detection platform, wherein the fourth instruction is used to control the first fluid device and the second fluid device to pump reagents required for the biochemical reaction from the sequencing reagent tank into the corresponding sequencing slide, so that the sample to be tested undergoes a biochemical reaction and enters the current gene sequencing cycle. Each fluid device corresponds to one sequencing slide. detecting the occupancy status of the XY platform in the gene sequencing system within a preset time before the biochemical reaction is completed; In a state where the XY platform is occupied by other sequencing slides, after waiting for the XY platform to be released, the first sequencing slide among the sequencing slides occupies the XY platform, and the computer in the gene sequencing system sends a fifth instruction to the XY platform in the gene sequencing system through the control device, wherein the fifth instruction is used to control the XY platform to move to the second position so that the area of ​​the first sequencing slide that needs to be detected by signal is directly below the objective lens in the Z direction, the objective lens is in the data acquisition module of the gene sequencing system, and the first sequencing slide is connected to the first biochemical reaction and detection platform or the Any sequencing slide corresponding to the second biochemical reaction and the detection platform; Step S23: After the biochemical reaction is completed, a sixth instruction is sent to the data acquisition module through the control device, wherein the sixth instruction is used to achieve focusing and signal acquisition on the first sequencing slide.

26. The method according to claim 25, characterized in that When there are multiple areas in the first sequencing slide that require signal collection, the fifth instruction controls the XY platform to move successively to positions where the second target areas are located directly below the objective lens in the Z direction, wherein the second target areas are multiple areas in the first sequencing slide that require signal collection.

27. The method according to claim 25, characterized in that The second sequencing process also includes: After completing signal acquisition for all regions requiring signal acquisition in the first sequencing slide, releasing the XY platform and determining whether all cycles of gene sequencing are completed, wherein the gene sequencing cycle includes step S22 and step S23; If all the gene sequencing cycles are not completed, return to step S22 to continue gene sequencing of the sample on the first sequencing slide. If all the gene sequencing cycles are completed, execute step S24. Step S24: Generate sequencing results of the first sequencing slide.

28. The method according to claim 21, wherein The third sequencing process is a process for performing gene sequencing on a third sequencing slide in any stage of performing gene sequencing on the second sequencing slide in the sequencing slides, wherein the stages of gene sequencing include a sequencing preparation stage, a biochemical reaction stage, a signal acquisition stage, and a sequencing result generation stage.

29. The method according to claim 28, characterized in that The gene sequencing process corresponding to the second sequencing slide includes: Step S31, receiving sequencing information filled in by a target subject, and controlling the lifting and lowering of a lifting screen according to the sequencing information so that the target subject is placed in a sequencing reagent tank and a second sequencing slide in the gene sequencing system; Step S32: Sending, by the control device, a seventh instruction to the fluidic device in the first target biochemical reaction and detection platform where the second sequencing slide is located, wherein the seventh instruction is used to control the fluidic device to pump reagents required for the biochemical reaction from the sequencing reagent reservoir into the second sequencing slide so that the sample to be tested undergoes a biochemical reaction and enters the current gene sequencing cycle, wherein the first target biochemical reaction and detection platform is either the first biochemical reaction and detection platform or the second biochemical reaction and detection platform; Within a preset time before the biochemical reaction is completed, the XY platform in the gene sequencing system is detected. Occupancy status; When the XY platform is occupied by another sequencing slide, after waiting for the XY platform to be released, the second sequencing slide occupies the XY platform, and the computer in the gene sequencing system sends an eighth instruction to the XY platform in the gene sequencing system through the control device, wherein the eighth instruction is used to control the XY platform to move to a third position so that the area of ​​the second sequencing slide requiring signal detection is directly below the objective lens in the Z direction, and the objective lens is in the data acquisition module of the gene sequencing system; Step S33: After the biochemical reaction is completed, a ninth instruction is sent to the data acquisition module via the control device, wherein the ninth instruction is used to achieve focusing and signal acquisition on the second sequencing slide.

30. The method according to claim 29, wherein The gene sequencing process corresponding to the second sequencing slide also includes: when there are multiple areas in the second sequencing slide that require signal acquisition, the computer controls the XY platform to move successively through the eighth instruction so that the third target area is successively located directly below the objective lens in the Z direction, wherein the third target area is a plurality of areas in the second sequencing slide that require signal acquisition.

31. The method according to claim 29, wherein The gene sequencing process corresponding to the second sequencing slide also includes: After completing signal acquisition for all regions requiring signal acquisition in the second sequencing slide, releasing the XY platform and determining whether all cycles of gene sequencing are completed, wherein the gene sequencing cycle includes step S32 and step S33; If all the gene sequencing cycles are not completed, return to step S32 to continue gene sequencing of the sample on the second sequencing slide. If all the gene sequencing cycles are completed, execute step S34. Step S34: Generate sequencing results for the second sequencing slide.

32. The method according to claim 28, wherein The gene sequencing process corresponding to the third sequencing slide includes: Step S41, receiving sequencing information filled in by the target subject, and detecting the occupancy status of the XY platform in the gene sequencing system; When the XY platform is occupied by another sequencing slide, the third sequencing slide occupies the XY platform after waiting for the XY platform to be released, and the lifting and lowering of the lifting screen is controlled according to the sequencing information so that the target object places the sequencing reagent tank and sequencing slide in the gene sequencing system, and the XY platform is released after the sequencing reagent tank and sequencing slide are placed in the gene sequencing system; Step S42: Sending a tenth instruction via the control device to the fluidic device in the second target biochemical reaction and detection platform where the third sequencing slide is located, wherein the tenth instruction is used to control the fluidic device to pump reagents required for the biochemical reaction from the sequencing reagent reservoir into the third sequencing slide so that the sample to be tested undergoes a biochemical reaction and enters the current gene sequencing cycle, wherein the second target biochemical reaction and detection platform is either the first biochemical reaction and detection platform or the second biochemical reaction and detection platform; detecting the occupancy status of the XY platform in the gene sequencing system within a preset time before the biochemical reaction is completed; When the XY platform is occupied by another sequencing slide, after waiting for the XY platform to be released, the third sequencing slide occupies the XY platform, and the computer in the gene sequencing system sends an eleventh instruction to the XY platform in the gene sequencing system through the control device, wherein the eleventh instruction is used to control the XY platform to move to a fourth position so that the area of ​​the third sequencing slide requiring signal detection is directly below the objective lens in the Z direction, and the objective lens is in the data acquisition module of the gene sequencing system; Step S43: After the biochemical reaction is completed, the control device sends a twelfth instruction to the data acquisition module, wherein the twelfth instruction is used to achieve focusing and signal acquisition on the third sequencing slide.

33. The method according to claim 32, characterized in that The gene sequencing process corresponding to the third sequencing slide also includes: when there are multiple areas in the third sequencing slide that need to perform signal acquisition, the computer controls the XY platform to move successively through the eleventh instruction so that the fourth target area is located directly below the objective lens in the Z direction in sequence, wherein the fourth target area is a plurality of areas in the third sequencing slide that need to perform signal acquisition.

34. The method according to claim 32, wherein The gene sequencing process corresponding to the third sequencing slide also includes: After completing signal acquisition for all regions requiring signal acquisition in the third sequencing slide, releasing the XY platform and determining whether all cycles of gene sequencing are completed, wherein the gene sequencing cycle includes step S42 and step S43; If all the gene sequencing cycles are not completed, return to step S42 to continue gene sequencing the sample on the third sequencing slide. If all the gene sequencing cycles are completed, execute step S44. Step S44: Generate the sequencing results of the third sequencing slide.

35. A gene sequencing device, characterized in that: include: a memory for storing program instructions; A processor, connected to the memory, and configured to execute the gene sequencing method according to any one of claims 21 to 34.

36. A non-volatile storage medium, characterized in that: The non-volatile storage medium includes a stored computer program, wherein the device where the non-volatile storage medium is located executes the gene sequencing method described in any one of claims 21 to 34 by running the computer program.

37. A computer program product comprising computer instructions, characterized in that When the computer instructions are executed by a processor, the gene sequencing method according to any one of claims 21 to 34 is implemented.

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