Model mouse with microglia-specific VDBP gene knocked out and construction method therefor

The vitamin D binding protein gene in microglia was specifically knocked out in a mouse model using the Cre-loxP recombination system, which solved the functional research difficulties caused by overall knockdown, achieved a knockdown efficiency of more than 80%, and supported more in-depth functional research.

WO2025213459A1 Publication Date: 2025-10-16SHENZHEN INST OF ADVANCED TECH
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Patent Information

Application Number
PCT/CN2024/087519
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Filing Date
2024-04-12
Publication Date
2025-10-16

AI Technical Summary

Technical Problem

Existing studies have knocked down the vitamin D binding protein gene as a whole in mice, making it difficult to study its function at specific locations.

Method used

Using the Cre-loxP recombination system, by constructing VDBPloxp/loxp gene mice and crossing them with Cre/ERT2 gene mice, the vitamin D binding protein gene in microglia was specifically knocked out, and the induced expression of the Cre gene was used to achieve specific knockdown of the vitamin D binding protein gene in microglia.

Benefits of technology

The specific knockdown efficiency of the vitamin D binding protein gene in microglia reached over 80%, which is conducive to more detailed research on the function of this molecule in the brain.

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Abstract

The present invention relates to a model mouse with a microglia-specific vitamin D binding protein (VDBP) gene knocked out and a construction method therefor. The construction method comprises the following steps: constructing a VDBPloxp / loxp gene mouse; hybridizing the VDBPloxp / loxp gene mouse with a Cre / ERT2 gene mouse to obtain a Cre+ / --VDBP+ / + gene mouse, wherein the microglia of the Cre / ERT2 gene mouse carry Cre gene; and inducing the expression of the Cre gene in the Cre+ / --VDBP+ / + gene mouse to obtain the model mouse with a microglia-specific VDBP gene knocked out. According to the construction method, a VDBP in the microglia of the model mouse can be specifically knocked out on the basis of Cre-loxP recombination, specific knockdown of the expression of the gene of the VDBP in the microglia is achieved, and the knockdown efficiency reaches 80% or more, facilitating more detailed study of the function of the molecule in the brain.
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