Method for in vitro mass proliferation and culturing of vδ1 t cells without need for artificial antigen-presenting cells

Abstract

The present invention relates to a method for mass proliferation and culturing of Vδ1 T cells, Vδ1 T cells produced by the method, and a use thereof, the method comprising a step for activating lymphocytes from a biological sample containing the lymphocytes, a step for isolating Vδ1 T cells from the activated cell population, and a step for proliferating and culturing the isolated Vδ1 T cells by treating the isolated Vδ1 T cells with an anti-TCRVδ1 antibody. The method for mass proliferation and culturing according to the present invention makes it possible to produce Vδ1 T cells without artificial antigen-presenting cells, while maintaining an excellent proliferation rate and purity, and thus can be effectively used in related technical fields such as cancer treatment using Vδ1 T cells.

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