Wireless portable compact cell incubator

Abstract

The present invention particularly relates to a wireless portable compact cell incubator. The wireless portable compact cell incubator comprises an incubator body, a control module and a power source, wherein a culture chamber, which is used for the placement of a cell culture dish, and a control chamber, which is used for the arrangement of the control module, are provided in the incubator body; the top and bottom of the incubator body that correspond to the culture chamber are both transparent; a measurement module and an environment regulation module, which are connected to the control module, are provided in the control chamber, the measurement module is used for measuring information about the temperature, the humidity and the content of bacteria in the culture chamber and transmitting same to the control module, and the control module controls the environment regulation module on the basis of the temperature and information about the content of bacteria in the culture chamber, so as to ensure that the temperature and the content of bacteria in the culture chamber are at set values; and the power source is electrically connected to the control module, the measurement module and the environment regulation module. By means of the technical solution, the present invention has the advantages of being compact, ensuring stable cell growth, reducing interference from an external environment, reducing cross-contamination, acquiring accurate and reproducible experimental results, and facilitating automated operations.

Description

Wireless portable small cell culture box TECHNICAL FIELD

[0001] The present application belongs to the technical field of cell culture, and particularly relates to a wireless portable small cell culture box. BACKGROUND

[0002] Cell experiments are conducted to study and understand the physiological, biochemical and genetic characteristics and functions of cells, and are one of important research means in the fields of biology and medicine, and are widely applied to disease research, biological research, immunological research and cell engineering, etc. In order to scientific research and medical development, a large number of cell culture experiments need to be conducted.

[0003] In the current laboratory, cell culture is mainly collective culture, that is, cells are placed in culture dishes to form cell monolayers or multilayers, and multilayer culture dishes are placed in a culture box for culture and research. In collective culture, the environment in the culture box is not uniform, and it is impossible to ensure that the temperature, humidity, gas environment and other conditions of each layer of culture dishes are completely consistent, which increases the heterogeneity of cell phenotypes and greatly affects the reproducibility of cell experiments.

[0004] In addition, during the process of cell culture, culture dishes need to be frequently taken out for observation. By observing the morphological, size, color and other characteristic changes of cells, the state of cell growth and differentiation can be understood, and the time for processing can be determined in a timely manner by finding out possible mutations or variations of cells. However, each time the culture dish is taken out for observation, the cells and culture are exposed to the external environment, which increases the risk of contamination by bacteria, fungi, viruses and other microorganisms, and further may lead to failure or variation of cell culture. Taking out the culture dish needs to open the culture box or incubator, which interrupts the growth and replication of cells in a constant environment. Cells cause stress reaction under the condition of changes in external environment and rapid changes in temperature and humidity, which may lead to cell damage, apoptosis or functional abnormalities, and finally leads to uncertainty of experiments. SUMMARY

[0005] In order to solve the problems in the related art, the present application provides a wireless portable small cell culture box, which solves the problems of environmental changes affecting normal growth and differentiation of cells and cells cultured in different growth environments affecting accuracy of experimental results.

[0006] The technical scheme is as follows:

[0007] The utility model provides a wireless portable small cell culture box, its characteristics are including box, control module and power, the culture cavity for placing cell culture dish and the control room for setting up control module are set up in the box, the culture cavity is correspondingly transparently set up in the top and bottom of the box, the detection module and the environmental regulation module who respectively with control module are set up in the control room, the detection module is used for detecting the temperature, humidity and bacterial content information in the culture cavity and passes temperature, humidity and bacterial content information to control module, and control module controls the environmental regulation module work according to temperature and bacterial content information in the culture cavity, ensures that the temperature and bacterial content in the culture cavity are at set value,

[0008] The power supply is a mobile power supply, which is electrically connected with the control module, the detection module and the environmental regulation module, and is used for providing working power for the control module, the detection module and the environmental regulation module.

[0009] Through the above technical scheme, by the transparent setting of the top and bottom of the box, when the cultured cells need to be observed in the middle, the cell culture dish does not need to be taken out, and the cells can be directly observed through the transparent top and bottom of the box, so that the growth environment of the subsequent cell culture can be ensured not to change, thereby avoiding the influence of environmental change on experimental data. At the same time, the control module obtains the temperature, humidity and bacterial content information in the culture cavity, and controls the environmental regulation module to adjust the temperature and bacterial content according to the information, so as to accurately ensure that all cells in the culture container are in the same growth environment, ensure that all cells are in a normal growth or differentiation state, and thus ensure the reliability of cell data.

[0010] Further, a base for charging the mobile power supply is further included, the top end of the base is provided with a clamping mechanism and an inflation mechanism, the bottom of the box corresponding to the culture cavity is provided with an inflation port, and the clamping mechanism is used to realize the connection between the box and the base, so that the inflation port is connected with the inflation mechanism.

[0011] Further, the box includes a cover plate rotatably arranged on the top of the culture cavity, the cover plate includes a support frame and a glass plate arranged in the support frame, the environmental regulation module includes a first heating plate and a second heating plate, the first heating plate is fixedly installed in the support frame below the glass plate in a transparent manner, and the second heating plate is fixedly installed on the bottom of the box corresponding to the culture cavity in a transparent manner.

[0012] Further, an air inlet communicating with the inflation port is arranged on one side vertical surface in the culture cavity, an air outlet is arranged on the other side vertical surface in the culture cavity, and a breathing valve is arranged on each of the air inlet and the air outlet.

[0013] Further, the front end and the rear end of the box are provided with clamping openings for facilitating the gripping of the mechanical hand.

[0014] Further, a moisturizing groove is arranged along the periphery of the inner bottom of the culture chamber, and a culture dish placement rack is arranged on the periphery of the inner wall of the culture chamber above the moisturizing groove.

[0015] Further, the control module comprises a touch screen arranged on the top of the box in the control chamber, a communication interface chip U37 and a main control chip arranged in the control chamber; the communication interface chip U37 and the main control chip are adaptively connected, the touch screen realizes communication interaction through the communication interface chip U37 and the main control chip; the environment adjusting module comprises two UVC ultraviolet lamps arranged in the culture chamber, and each of the two UVC ultraviolet lamps is connected with a communication isolation unit; the main control chip controls the opening and closing of the two UVC ultraviolet lamps by controlling the two communication isolation units.

[0016] Further, the main control chip is adaptively connected with a 485 communication unit one and a 485 communication unit two; the first heating plate and the second heating plate are respectively connected with a temperature control unit one and a temperature control unit two, the temperature control unit one is connected with the port P7 of the 485 communication unit one, and the temperature control unit two is connected with the port P14 of the 485 communication unit two; the main control chip controls the 485 communication unit one and the 485 communication unit two to work, so as to control the opening and closing of the first heating plate and the second heating plate.

[0017] Further, a one-key switch and a sterilization switch are arranged on one side of the top of the box, the one-key switch is connected with the control module, the detection module and the environment adjusting module, and the sterilization switch is connected with the UVC ultraviolet lamp.

[0018] Further, the base is provided with a gas path pipeline for providing a gas source for the inflation mechanism.

[0019] In summary, the beneficial effects of the present application are that, by arranging the top and bottom of the box to be transparent, when it is necessary to observe the cultured cells in the middle, the cells can be directly observed through the transparent top and bottom of the box without taking out the cell culture dish, so that the growth environment of the subsequent cell culture can be ensured not to change, thereby avoiding the influence of environmental changes on experimental data. At the same time, the control module obtains the temperature, humidity and bacterial content information in the culture chamber, and adjusts the temperature and bacterial content according to the information, so as to accurately ensure that all cells in the culture container are in the same growth environment, ensure that all cells are in a normal growth or differentiation state, and ensure the reliability of cell data.

[0020] It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the application, as claimed. BRIEF DESCRIPTION OF DRAWINGS

[0021] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the application and serve to explain the principles of the application, in which, like reference numerals designate corresponding parts throughout the several views.

[0022] FIG. 1 is a structural schematic diagram of the present application;

[0023] FIG. 2 is a top view of the box in the present application;

[0024] FIG. 3 is a sectional view in the direction of A-A in FIG. 2;

[0025] FIG. 4 is a structural schematic diagram of the box in the present application;

[0026] FIG. 5 is a structural schematic diagram of the base in the present application;

[0027] FIG. 6 is a schematic diagram of the inside of the culture chamber in the present application;

[0028] FIG. 7 is a schematic block diagram of the control module in the present application;

[0029] FIG. 8 is an internal schematic diagram of the main control chip in the present application;

[0030] FIG. 9 is an internal connection schematic diagram of the 485 communication unit one and the 485 communication unit two in the present application;

[0031] FIG. 10 is an internal connection schematic diagram of the touch screen in the present application;

[0032] FIG. 11 is an internal connection schematic diagram of the UVC ultraviolet lamp in the present application;

[0033] In the drawings, 1 is the box; 2 is the base; 3 is the culture chamber; 4 is the control chamber; 5 is the cover plate; 501 is the frame; 502 is the glass one; 6 is the clamping mechanism; 7 is the inflation port; 8 is the breather valve; 9 is the clamping port; 10 is the culture vessel mounting rack; 11 is the touch screen; 12 is the one-key switch; 13 is the sterilization button; 14 is the first heating plate; 15 is the second heating plate; 16 is the humidification tank. DETAILED DESCRIPTION

[0034] The exemplary embodiments will be described in detail herein below with reference to the drawings. In the following description, the same drawings reference numbers are used to denote like or similar elements unless otherwise denoted. The embodiments described in the following exemplary embodiments are not representative of all embodiments consistent with the present application. Rather, they are merely examples of devices and methods consistent with some aspects of the present application as detailed in the appended claims.

[0035] In one possible embodiment, as shown in Figures 1-11, a wireless portable small cell culture box includes a box body 1, a control module and a power supply, the box body 1 is provided with a culture chamber 3 for placing cell culture dishes and a control chamber 4 for setting the control module; the culture chamber 3 is transparently arranged at the top and bottom of the box body 1; the control chamber 4 is provided with a detection module and an environmental adjustment module connected with the control module respectively, the detection module is used to detect the temperature, humidity and bacterial content information in the culture chamber 3, and transmit the temperature, humidity and bacterial content information to the control module, the control module controls the environmental adjustment module to work according to the temperature and bacterial content information in the culture chamber 3, to ensure that the temperature and bacterial content in the culture chamber 3 are at the set value;

[0036] The power supply is a mobile power supply, which is electrically connected with the control module, the detection module and the environmental adjustment module, and is used to provide working power for the control module, the detection module and the environmental adjustment module. The mobile power supply is a rechargeable power supply.

[0037] The front end and the rear end of the box body 1 are provided with clamping openings 9 for facilitating the gripping of the mechanical hand, which facilitates the gripping and moving of the culture box by the mechanical arm.

[0038] A humidifying groove 16 is arranged along the bottom periphery of the culture chamber 3, and a culture dish mounting rack 10 is arranged on the periphery of the inner wall of the culture chamber 3 above the humidifying groove 16. The detection module includes a temperature sensor, a humidity sensor and a gas sensor. When the humidity value in the culture chamber 3 is detected to be less than the set threshold value of the control module, water is manually added to the humidifying groove 16.

[0039] The box body 1 includes a cover plate 5 rotatably arranged at the top of the culture chamber, the cover plate 5 includes a support frame 501 and a glass 502 arranged in the support frame 501; the environmental adjustment module includes a first heating plate 14 and a second heating plate 15, the first heating plate 14 is fixedly installed in the support frame 501 below the glass 502, and the second heating plate 15 is fixedly installed at the bottom of the box body 1 corresponding to the culture chamber 3.

[0040] An air inlet is arranged on one side of the vertical surface in the culture chamber 3 and communicates with the air inlet 7, and an air outlet is arranged on the other side of the vertical surface in the culture chamber 3, a breathing valve 8 is arranged on the air inlet and the air outlet respectively, the specific structure and installation method of the breathing valve 8 belong to the prior art, and will not be described in detail here; the model selected can only be breathable and cannot pass through liquid, which belongs to the public knowledge and will not be described here; the breathing valve 8 includes a mounting frame, one or more first holes are arranged in the mounting frame. The first hole is adapted to the air inlet, a breathable membrane is arranged in the first hole, and a sealing ring is arranged along the periphery of the first hole. A handle is fixedly arranged on one side of the first hole, and a second hole adapted to the handle is arranged on the top of the air inlet and the air outlet.

[0041] The control module comprises a touch screen 11 arranged at the top of the box 1 corresponding to the control chamber 4, a communication interface chip U37 and a main control chip arranged in the control chamber 4; the communication interface chip U37 and the main control chip are adaptively connected, and the touch screen 11 realizes communication interaction through the communication interface chip U37 and the main control chip; the internal structure and specific connection of the communication interface chip U37 and the main control chip are shown in Figures 10 and 8, which belong to the prior art and will not be described in detail here.

[0042] The environmental regulation module comprises two UVC ultraviolet lamps arranged in the culture chamber 3, and each of the two UVC ultraviolet lamps is connected with a communication isolation unit; the main control chip controls the opening and closing of the two UVC ultraviolet lamps by controlling the two communication isolation units; the internal structure and specific connection of the two communication isolation units and the main control chip are shown in Figures 9 and 8, which belong to the prior art and will not be described in detail here.

[0043] The main control chip is adaptively connected with a 485 communication unit one and a 485 communication unit two; the first heating plate 14 and the second heating plate 15 are respectively connected with a temperature control unit one and a temperature control unit two, and the port P7 of the temperature control unit one and the 485 communication unit one are connected; the port P14 of the temperature control unit two and the 485 communication unit two are connected; the specific internal principle diagram of the 485 communication unit one and the 485 communication unit two is shown in Figure 11, and the connection of the main control chip and the first heating plate 14 and the second heating plate 15 all belong to the prior art and will not be described in detail here.

[0044] A one-key switch 12 and a sterilization switch are arranged at the top of the box 1 on one side of the touch screen 11; the one-key switch 12 is connected with the control module, the detection module and the environmental regulation module, and the sterilization switch is connected with the UVC ultraviolet lamp.

[0045] The sterilization button 13 is used for one-key opening and closing of the UVC ultraviolet lamp, and the one-key switch 12 is used for one-key control of the opening and closing of the touch screen 11, the temperature sensor, the humidity sensor, the gas sensor, the UVC ultraviolet lamp, the communication chip P7 and the communication chip P14.

[0046] It also comprises a base 2 for charging the mobile power supply, the top end of the base 2 is provided with a clamping mechanism 6 and an inflation mechanism, and the bottom of the culture chamber 3 corresponding to the box 1 is provided with an inflation port 7; the clamping mechanism is used to realize the connection of the box 1 and the base 2, so that the inflation port 7 and the inflation mechanism are connected.

[0047] The clamping mechanism 6 comprises two limiting cones symmetrically arranged on the top of the base 2, and the bottom of the box 1 is correspondingly provided with limiting holes; the charging mechanism comprises a quick connector arranged between the two limiting cones and a rubber ring arranged along the periphery of the quick connector; the quick connector penetrates through the base 2 and is provided with a circular groove along the periphery of the inflation port 7, the circular groove and the rubber ring are matched, and a pipeline for inflating the quick connector is arranged in the base 2; the charging mechanism comprises a fixing sheet arranged on the top of the base 2, spring pins arranged on the top of the fixing sheet, and charging sockets arranged between the spring pins; the fixing sheet is arranged on one side of one of the limiting cones, one end of the charging socket is connected with an external power supply, and the other end of the charging socket is used for charging the mobile power supply; the box 1 at the bottom of the mobile battery is provided with a charging port corresponding to the position of the charging socket and matched with the charging socket; when charging is needed, the limiting hole is placed on the limiting cone, at this time, the quick connector and the charging port are connected, and the spring pin and the charging port are connected.

[0048] A gas pipeline for providing gas source for the inflation mechanism is arranged in the base 2.

[0049] Principle: Close the cover plate 5 to make the culture chamber 3 in a closed state. Click the one-key switch 12 to make the box 1 start running. The culture chamber 3 detects the temperature, humidity and bacterial composition in the culture chamber 3 in the running state, and sends the detection data to the control module through the wireless module.

[0050] The data detected by the detection module is displayed to the user through the touch screen 11, the control module compares the detection data with the set value to adjust the temperature and bacterial information in the culture chamber 3, and then slides the touch screen 11 to issue an adjustment instruction to the control module to issue a control instruction, and then controls the power-on and power-off of the first heating plate 14 and the second heating plate 15, and the opening and closing of the UVC ultraviolet lamp; according to the humidity information detected by the detection module, water is added to the humidifying tank 16 by manual.

[0051] During normal growth and differentiation of cells, the whole device is placed under the observation device by using an external mechanical arm to clamp the clamping port 9. In addition, since the top and bottom glasses in the device are transparent glasses, when it is necessary to observe the cultured cells in the middle, the cells can be directly observed through the transparent box 1 top, so that the growth environment of the subsequent cell culture can be ensured not to change, thereby avoiding the influence of environmental changes on experimental data.

[0052] The single-layer culture dish and the environmental regulation module are arranged to allow the control module to obtain the temperature, humidity and gas composition information in the culture chamber 3, and control the environmental regulation module according to the information, so as to accurately ensure that all cells on the culture dish are in the same growth environment, and then ensure that all cells can grow and differentiate normally, thereby ensuring the reliability of the cell experimental data.

[0053] Other embodiments of the application will be apparent to those skilled in the art from consideration of the specification and practice of the application disclosed herein. It is intended that the application be construed as including any patent, any patent applications, and any patent publications to the extent that such alias are equivalent to the content of the acknowledged but not yet granted patents or patent applications. It is intended and it is specifically disclosed that various modifications and variations can be made in the details of design and implementation of the present application without departing from the spirit or scope of this application. Further, it is to be understood that the application can be practiced otherwise than is specifically described in the accompanying examples and that any or all activities specifically described in the examples can be employed as combinations with one another in the practice of the application, unless otherwise specified herein. It is therefore intended that the specification and examples be considered as exemplary only, with the true scope and spirit of the application being indicated by the following claims.

[0054] It is to be understood that the application is not limited to the precise details of design and operation as herein described and illustrated in the drawings; various modifications and changes can be made within the scope and spirit of the application. The scope of the application is limited only by the claims that follow.

Claims

1. A wireless portable compact cell culture incubator, characterized by: The application relates to a cell culture box, which comprises a box body, a control module and a power supply, a culture cavity for placing cell culture dishes and a control chamber for arranging the control module are arranged in the box body; the culture cavity is transparently arranged at the top and the bottom of the corresponding box body; a detection module and an environment adjusting module which are connected with the control module are arranged in the control chamber; the detection module is used for detecting the temperature, humidity and bacterial content information in the culture cavity and transmitting the temperature, humidity and bacterial content information to the control module; the control module controls the environment adjusting module to work according to the temperature and the bacterial content information in the culture cavity, so that the temperature and the bacterial content in the culture cavity are kept at the set values; The power supply is a mobile power supply which is electrically connected with the control module, the detection module and the environment adjusting module and is used for providing working power for the control module, the detection module and the environment adjusting module.

2. The wireless portable compact cell culture incubator according to claim 1, wherein, The application further relates to a base used for charging the mobile power supply, a clamping mechanism and an inflation mechanism are arranged at the top end of the base; an inflation port is arranged at the bottom of the corresponding box body of the culture cavity; the clamping mechanism is used for connecting the box body and the base so that the inflation port is connected with the inflation mechanism.

3. The wireless portable compact cell culture incubator of claim 1, wherein, The box body comprises a cover plate which is rotatably arranged at the top of the culture cavity; the cover plate comprises a support frame and a glass I arranged in the support frame; The environment adjusting module comprises a first heating plate and a second heating plate; the first heating plate is fixedly arranged in the support frame below the glass I in a transparent mode; the second heating plate is fixedly arranged at the bottom of the corresponding box body of the culture cavity in a transparent mode.

4. The wireless portable compact cell culture incubator according to claim 2, wherein, An air inlet which is communicated with the inflation port is arranged on one side vertical surface in the culture cavity; an air outlet is arranged on the other side vertical surface in the culture cavity; a breathing valve is arranged on the air inlet and the air outlet respectively.

5. The wireless portable compact cell culture incubator of claim 1, wherein, Clamping ports which are convenient for being gripped by a mechanical hand are arranged at the front end and the rear end of the box body.

6. The wireless portable compact cell culture incubator of claim 1, wherein, A humidifying groove is arranged along the bottom periphery of the culture cavity; an inner wall periphery of the culture cavity above the humidifying groove is provided with a culture dish placing rack.

7. The wireless portable compact cell culture incubator according to claim 3, wherein The control module comprises a touch screen arranged at the top of the corresponding box body of the control chamber, a communication interface chip and a main control chip arranged in the control chamber; The communication interface chip and the main control chip are adaptively connected; the touch screen realizes communication interaction through the communication interface chip and the main control chip; The environment adjusting module comprises two UVC ultraviolet lamps arranged in the culture cavity; the two UVC ultraviolet lamps are connected with communication isolation units; the main control chip controls the opening and closing of the two UVC ultraviolet lamps through the two communication isolation units.

8. A wireless portable compact cell culture incubator according to claim 7, wherein, The main control chip is connected with the first heating plate through a 485 communication unit one; the main control chip controls the working or stopping of the first heating plate through the 485 communication unit one; the main control chip is connected with the second heating plate through a 485 communication unit two; the main control chip controls the working or stopping of the second heating plate through the 485 communication unit two.

9. The wireless portable compact cell culture incubator of claim 7, wherein, A one-key switch and a sterilization switch are arranged at the top of the box body on one side of the touch screen; the one-key switch is connected with the control module, the detection module and the environment adjusting module; the sterilization switch is connected with the UVC ultraviolet lamp.

10. The wireless portable compact cell culture incubator of claim 2, wherein, The base is provided with a gas path pipeline for providing a gas source for the inflating mechanism.

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