Industrial-scale preparation system for enzymatically hydrolyzed casein polypeptides, and control method therefor

Abstract

Provided are an industrial-scale preparation system for enzymatically hydrolyzed casein polypeptides, and a control method therefor. The preparation system comprises one or more enzymatic hydrolysis tanks and one or more first external circulation pipes. Each enzymatic hydrolysis tank comprises a heat exchange jacket and a differential stirring device. Each differential stirring device comprises a main shaft, a plurality of main impellers, a plurality of auxiliary shafts, a plurality of auxiliary impellers and an electric motor. The main shafts and the auxiliary shafts have different rotational speeds to create differential rotation for slowing down the formation of casein micelles. Two ends of each first external circulation pipe are respectively connected to the bottom and the upper part of each enzymatic hydrolysis tank, such that a material in each enzymatic hydrolysis tank is extracted from the bottom of each enzymatic hydrolysis tank and then introduced into each enzymatic hydrolysis tank from the upper part of each enzymatic hydrolysis tank, thereby creating circulation of the material in the vertical direction and outside of each enzymatic hydrolysis tank to improve the enzymatic hydrolysis efficiency. By means of providing the main shafts and the auxiliary shafts that have different rotational speeds, and the first external circulation pipes, the preparation system can effectively enhance the fluidity of the material in the enzymatic hydrolysis tanks, slow down the formation of the casein micelles and improve the enzymatic hydrolysis efficiency.

Description

Industrial preparation system of enzymatic casein polypeptide and control method thereof TECHNICAL FIELD

[0001] The present application relates to the technical field of enzymatic device, and particularly relates to an industrial preparation system of enzymatic casein polypeptide and a control method thereof. BACKGROUND

[0002] Milk protein is a general term for a variety of protein mixtures in a milk system, and milk protein is mainly composed of casein and whey protein. Among them, casein can be used to make cheese, and the main consumption form of cheese is original cheese, reprocessed cheese and cheese products mainly as snack foods, and the market scale is relatively small. In contrast, the formula milk powder market is broad, and the consumption of whey protein is huge. Based on the above objective reasons, casein has become an important by-product of the dairy deep processing industry, and the high value-added application of casein has become a major strategic project for the dairy industry in the future.

[0003] Small molecule peptides produced by casein hydrolysis have multiple functions in vivo, such as participating in immune regulation, scavenging free radicals, regulating blood pressure balance, anti-thrombosis and strengthening bone health, and casein has low allergenicity and easy absorption, so it has high nutritional value and market value. However, the hydrolysis process of casein is very unstable, and it is easy to form casein micelles and precipitate, and casein hydrolysis will cause a large number of hydrophobic amino acids to be exposed, resulting in a bitter taste of the hydrolysis product, and any carelessness in the production process will cause the casein final product to be unusable. Therefore, the taste, molecular weight distribution, polypeptide content and other indicators of enzymatic casein polypeptide in the production of casein enzymolysis (enzymolysis: hydrolysis under the action of protease) must have high batch-to-batch stability in industrial application.

[0004] However, in the current large-scale industrial production of casein polypeptide, there is no special enzymatic system for casein polypeptide, and the existing enzymatic equipment has low control precision of reaction conditions such as pH value and temperature, poor in-tank circulation and stirring shear force, slow enzyme inactivation heating and other problems. Specifically, the existing general enzymatic equipment commonly used in the existing process scheme mainly has the following problems: 1. During the enzymatic process, casein is easily aggregated to form larger casein micelles, which can cause a series of problems such as lumping, precipitation and wall sticking, resulting in low enzymatic efficiency, poor product solubility, and large fluctuations in degree of hydrolysis and molecular weight distribution between batches. 2. During the pH adjustment process, the addition amount of acid and alkali is not uniform, which can easily cause local pH to be too high or too low in the solution system, resulting in denaturation and precipitation of casein. 3. Low enzymatic reaction efficiency. Since the substrate concentration of the enzymatic solution is generally between 10% and 20%, the traditional enzymatic system mainly relies on the disturbance generated by the stirring paddle in the tank to make the enzyme react with the substrate, resulting in low enzymatic efficiency and large enzyme consumption. 4. Slow system heating rate. The existing system generally needs more than 30 minutes to raise the temperature from 60°C to 90°C. Since the overall enzymatic time is generally about 40 minutes, longer heating time can easily lead to unstable product batches in the enzymatic casein polypeptide production process.

[0005] Based on the above problems of the prior art, it is necessary to optimize the existing preparation system to adapt to the industrial production of large-scale enzymatic casein, and at least to improve some of the technical problems of the prior art. SUMMARY

[0006] The present application is made in view of the above state of the art. The purpose of the present application is to provide an industrial preparation system for enzymatic casein polypeptide to reduce the generation of casein micelles in the enzymatic casein process and improve the enzymatic efficiency.

[0007] The present application also provides a control method for the above-mentioned industrial preparation system for enzymatic casein polypeptide.

[0008] The application provides an industrial preparation system for enzymatic hydrolysis of casein polypeptide, which comprises one or more enzymatic hydrolysis tanks and one or more first external circulation pipelines, the enzymatic hydrolysis tank comprises a heat exchange jacket arranged on the wall of the enzymatic hydrolysis tank for regulating the temperature of the material in the enzymatic hydrolysis tank and a differential speed stirring device, the differential speed stirring device comprises a main shaft, a plurality of main paddles connected to the main shaft, a plurality of auxiliary shafts, a plurality of auxiliary paddles connected to the auxiliary shafts and a motor, the motor is connected to the main shaft or one of the auxiliary shafts, at least one of the auxiliary shafts is in driving connection with the main shaft, so that one of the motors drives the main shaft and the auxiliary shafts to rotate, the rotation speed of the main shaft is different from that of the auxiliary shafts, so as to form differential speed rotation for slowing down the formation of casein micelles, the two ends of the first external circulation pipeline are connected to the bottom and the upper part of the enzymatic hydrolysis tank respectively, and the first external circulation pipeline is provided with a first circulation pump, so that the material in the enzymatic hydrolysis tank is extracted from the bottom of the enzymatic hydrolysis tank and then introduced into the enzymatic hydrolysis tank from the upper part of the enzymatic hydrolysis tank, thereby forming the circulation of the material in the upward and downward directions and the outside of the enzymatic hydrolysis tank to improve the enzymatic hydrolysis efficiency.

[0009] In at least one possible implementation, the motor is connected to the main shaft, the auxiliary shafts are connected to the main shaft through connecting rods, so that the auxiliary shafts can rotate around the main shaft, the differential speed stirring device comprises a main wheel connected to the main shaft, an auxiliary wheel connected to the auxiliary shafts and a transmission belt or a transmission chain, the transmission belt is sleeved on the main wheel and the auxiliary wheel, so that the auxiliary wheel rotates with the rotation of the main wheel, the diameter of the main wheel is greater than that of the auxiliary wheel, so that the rotation speed of the main shaft is less than that of the auxiliary shafts, and the main shaft is arranged closer to the central axis of the enzymatic hydrolysis tank than the auxiliary shafts.

[0010] In at least one possible implementation, the differential speed stirring device further comprises a scraper connected to the main shaft and rotating with the main shaft, and the scraper abuts against the inner wall of the enzymatic hydrolysis tank to clean the material on the inner wall of the enzymatic hydrolysis tank.

[0011] In at least one possible implementation, the first external circulation pipeline is further provided with a pipeline type extrusion impactor, the pipeline type extrusion impactor comprises joints arranged at both ends of the pipeline type extrusion impactor to connect pipelines, a variable diameter pipe arranged inside the joint, an extrusion head arranged inside the variable diameter pipe and having a nozzle capable of extruding material, and a turbulence groove arranged in the middle of the pipeline type extrusion impactor.

[0012] In at least one possible implementation, the industrial preparation system for enzymatic hydrolysis of casein polypeptides further comprises a second external circulation pipeline, which is provided with a second circulation pump and an external heat exchanger, and two ends of the second external circulation pipeline are connected to the bottom and the upper part of the enzymatic hydrolysis tank respectively, so that the material in the enzymatic hydrolysis tank is extracted from the bottom of the enzymatic hydrolysis tank, and then the temperature of the material is regulated by the external heat exchanger, and finally the material is introduced into the enzymatic hydrolysis tank from the upper part of the enzymatic hydrolysis tank.

[0013] In at least one possible implementation, the industrial preparation system for enzymatic hydrolysis of casein polypeptides comprises a plurality of the enzymatic hydrolysis tanks and a plurality of the first external circulation pipelines, each of the first external circulation pipelines is connected to one of the enzymatic hydrolysis tanks, the plurality of the enzymatic hydrolysis tanks are connected in parallel, and the plurality of the enzymatic hydrolysis tanks are connected to one of the second external circulation pipelines.

[0014] In at least one possible implementation, the industrial preparation system for enzymatic hydrolysis of casein polypeptides further comprises an acid-alkali configuration device, which is connected to the enzymatic hydrolysis tank, and a three-fluid atomizing nozzle is arranged at the end of the pipeline connected to the enzymatic hydrolysis tank, the three-fluid atomizing nozzle comprises an inner air pipe, an acid-alkali liquid pipe and an outer air pipe, the inner air pipe is located radially inside the acid-alkali liquid pipe, the acid-alkali liquid pipe is located radially inside the outer air pipe, an end of the inner air pipe is provided with a cyclone plate to form a gas cyclone, the acid-alkali liquid pipe can pass acid or alkali liquid, and the inner air pipe and the outer air pipe can pass air, so that the acid or alkali liquid can form atomized droplets under the action of air flow.

[0015] In at least one possible implementation, the industrial preparation system for enzymatic hydrolysis of casein polypeptides further comprises a sterilization device and one or more enzyme addition tanks, the sterilization device is connected to the enzymatic hydrolysis tank to introduce casein solution treated by sterilization into the enzymatic hydrolysis tank, and the enzyme addition tank is connected to the enzymatic hydrolysis tank to introduce enzyme preparation into the enzymatic hydrolysis tank.

[0016] The application provides a control method of an industrial preparation system for enzymatic hydrolysis of casein polypeptides, which is applied to the above-mentioned industrial preparation system for enzymatic hydrolysis of casein polypeptides, and in the enzymolysis process of casein polypeptides, the differential speed stirring device of the enzymatic hydrolysis tank and the first external circulation pipeline are operated at least partially simultaneously to make the material in the enzymatic hydrolysis tank flow.

[0017] The application also provides a control method of an industrial preparation system for enzymatic hydrolysis of casein polypeptides, which is applied to the above-mentioned industrial preparation system for enzymatic hydrolysis of casein polypeptides, and in the enzyme inactivation process of casein polypeptides, the heat exchange jacket of the enzymatic hydrolysis tank and the second external circulation pipeline are operated simultaneously to make the material in the enzymatic hydrolysis tank warm up.

[0018] The enzymatic casein polypeptide industrial preparation system and the control method thereof provided by the application can effectively enhance the flowability of the material in the enzyme hydrolysis tank, thereby enhancing the shearing force on the casein, slowing down the formation of casein micelles, and reducing or avoiding the formation of larger casein micelles in the enzyme hydrolysis tank. At the same time, the technical solution can also improve the enzyme hydrolysis efficiency of the enzyme hydrolysis tank, thereby reducing the enzyme dosage and improving the production efficiency of the casein polypeptide product. BRIEF DESCRIPTION OF DRAWINGS

[0019] FIG. 1 is a structural schematic diagram of a preparation system according to an embodiment of the application.

[0020] FIG. 2 is a structural schematic diagram of an enzyme addition tank according to an embodiment of the application.

[0021] FIG. 3 is a structural schematic diagram of an acid-alkali configuration device according to an embodiment of the application.

[0022] FIG. 4 is a structural schematic diagram of an enzyme hydrolysis tank group according to an embodiment of the application.

[0023] FIG. 5 is a structural schematic diagram of an enzyme hydrolysis tank according to an embodiment of the application.

[0024] FIG. 6 is a structural schematic diagram of the internal structure of an enzyme hydrolysis tank according to an embodiment of the application.

[0025] FIG. 7 is a structural schematic diagram of a partial structure of an enzyme hydrolysis tank according to an embodiment of the application.

[0026] FIG. 8 is a structural schematic diagram of a partial structure of an enzyme hydrolysis tank from another perspective according to an embodiment of the application.

[0027] FIG. 9 is a structural schematic diagram of a pipeline type extrusion impactor according to an embodiment of the application.

[0028] FIG. 10 is a structural schematic diagram of a three-fluid atomizing nozzle according to an embodiment of the application.

[0029] FIG. 11 is a structural schematic diagram of a partial structure of a three-fluid atomizing nozzle according to an embodiment of the application.

[0030] Legend 10 sterilization equipment 20 enzyme addition tank 30 acid and alkali preparation equipment 31 concentrated acid tank 32 concentrated alkali tank 33 dilute alkali tank 34 dilute acid tank 40 enzymatic hydrolysis tank 41 heat exchange jacket 42 differential speed stirring device 421 main shaft 422 main paddle 423 auxiliary shaft 424 auxiliary paddle 425 motor 426 main wheel 427 auxiliary wheel 428 transmission belt 429 scraper 50 external heat exchanger 61 first external circulation pipeline 611 pipeline extrusion impactor 6111 joint 6112 variable diameter pipe 6113 extrusion head 6114 turbulence groove 62 second external circulation pipeline 71 first circulation pump 72 second circulation pump 80 three-fluid atomizing nozzle 81 air inner tube 82 acid and alkali liquid tube 83 air outer tube 84 rotating vane DETAILED DESCRIPTION

[0031] The exemplary embodiments of the present application will be described below with reference to the accompanying drawings. It is to be understood that the specific description is only for teaching those skilled in the art how to implement the present application, and is not intended to exhaust all possible ways of the present application, nor to limit the scope of the present application.

[0032] The connection referred to in the present application includes direct connection and indirect connection through pipes, lines, transmission members, etc.

[0033] The embodiments of the present application provide an industrial preparation system for enzymatic hydrolysis of casein polypeptide (hereinafter, sometimes referred to as "preparation system" for short), as shown in FIG. 1, which can include sterilization equipment 10, enzyme addition tank 20, acid and alkali preparation equipment 30, and one or more enzymatic hydrolysis tanks 40.

[0034] The sterilization equipment 10 can be a pasteurization equipment, which can be understood to be suitable for sterilization of dairy products and emulsion by-products. As shown in FIG. 1, the sterilization equipment 10 can be connected to the enzymatic hydrolysis tank 40 through a pipe, and the casein solution can enter the enzymatic hydrolysis tank 40 after being subjected to (pasteurization) sterilization treatment by the sterilization equipment 10.

[0035] As shown in FIGS. 1 and 2, the preparation system can include one or more enzyme addition tanks 20, and in the example, two enzyme addition tanks 20 are provided in the embodiments. The plurality of enzyme addition tanks 20 can be connected in parallel. Enzyme preparation and water can be added to the enzyme addition tank 20 to allow the enzyme preparation to be fully dissolved in the enzyme addition tank 20. It can be understood that the water used in the embodiments can be reverse osmosis water (i.e., RO water, which is filtered by reverse osmosis through a semi-permeable membrane, and can remove most of the ions, microorganisms, and other impurities in the water). The enzyme addition tank 20 can be connected to the enzymatic hydrolysis tank 40 by a pump (particularly a centrifugal pump) to transport the dissolved enzyme preparation. The enzyme addition tank 20 can also be provided with a high liquid level indicator (LSH), a low liquid level indicator (LSL), a weight transmitter (WT), etc.

[0036] As shown in FIG. 1 and FIG. 3, the acid-base configuration device 30 can include a concentrated acid tank 31, a concentrated alkali tank 32, a dilute alkali tank 33 and a dilute acid tank 34. Reverse osmosis water and nitric acid solution can be added to the concentrated acid tank 31, and reverse osmosis water and food-grade sodium hydroxide (solid) can be added to the concentrated alkali tank 32, respectively, and fully stirred and dissolved. The concentrated acid tank 31 can be connected to the dilute acid tank 34 by a pump (in particular, a diaphragm pump), and the concentrated alkali tank 32 can be connected to the dilute alkali tank 33 by a pump (in particular, a diaphragm pump) to pass the concentrated acid solution and the concentrated alkali solution into the dilute acid tank 34 and the dilute alkali tank 33, respectively, for accurate configuration. The concentrated acid tank 31 and the concentrated alkali tank 32 can be respectively provided with a weight transmitter (WT).

[0037] The dilute alkali tank 33 and the dilute acid tank 34 can be respectively connected to the enzymolysis tank 40 to regulate the pH value (acidity and alkalinity) of the enzymolysis solution in the enzymolysis tank 40. The dilute alkali tank 33 and the dilute acid tank 34 can respectively pass reverse osmosis water, and be respectively provided with a high liquid level indicator (LSH), a low liquid level indicator (LSL), a weight transmitter (WT) and a conductivity transmitter (CT for measuring ion concentration) to achieve accurate pH value regulation. The dilute alkali tank 33 and the dilute acid tank 34 can be respectively connected to the enzymolysis tank 40 by a pump (in particular, a centrifugal pump), and a flow meter (in particular, an electromagnetic flow meter) can be further provided in the connecting pipeline to respectively measure the flow of the acid solution and the alkali solution.

[0038] As shown in FIG. 1, FIG. 4 and FIG. 5, the enzymolysis tank 40 can be provided with a heat exchange jacket 41 and a differential speed stirring device 42. The heat exchange jacket 41 can be arranged on the wall of the enzymolysis tank 40 to regulate the temperature of the material in the enzymolysis tank 40. Preferably, the heat exchange jacket 41 can be a steam heating jacket, and the temperature in the enzymolysis tank 40 can be raised by passing steam into the heat exchange jacket 41. The heat exchange jacket 41 can be provided with a steam inlet (LS) and a condensate outlet (SC) for passing steam and discharging steam condensate, respectively. The differential speed stirring device 42 can stir the material in the enzymolysis tank 40 by a plurality of stirring paddles with different rotation speeds (in FIG. 5, the differential speed stirring device is indicated by a symbol). The enzymolysis tank 40 can be provided with a high liquid level indicator (LSH), a low liquid level indicator (LSL), a pH value transmitter (PH) and a temperature transmitter (TT). A plurality of enzymolysis tanks 40 can be connected in parallel to form an enzymolysis tank group. For example, three parallel enzymolysis tanks 40 are provided in the embodiment, and it can be understood that the parallel enzymolysis tanks 40 can share part of the pipeline.

[0039] Specifically, as shown in FIGS. 6, 7 and 8, the differential stirring device 42 can include a main shaft 421, main paddles 422, a secondary shaft 423, secondary paddles 424, a motor 425, a main wheel 426, a secondary wheel 427 and a transmission belt 428. The central shaft 421 can be connected to a plurality of main paddles 422. For example, the central shaft 421 in the present embodiment is connected to 10 main paddles 422 (including 5 groups of main paddle groups arranged at intervals, and each main paddle group includes 2 main paddles). The secondary shaft 423 can be connected to a plurality of secondary paddles 424. For example, the secondary shaft 423 in the present embodiment is connected to 8 secondary paddles 424 (including 4 groups of secondary paddle groups arranged at intervals, and each secondary paddle group includes 2 secondary paddles). The motor 425 can be connected to the main shaft 421 to drive the main shaft 421 to rotate. The secondary shaft 423 can be connected to the main shaft 421 through a connecting rod, so that when the main shaft 421 rotates, the secondary shaft 423 can rotate around the main shaft 421 (i.e., the secondary shaft 423 can perform a revolution motion around the main shaft 421).

[0040] The main wheel 426 can be arranged on the main shaft 421, and the main wheel 426 can rotate with the main shaft 421. The secondary wheel 427 can be arranged on the secondary shaft 423, and the secondary wheel 427 can rotate with the secondary shaft 423. The main wheel 426 and the secondary wheel 427 can be connected through a transmission belt 428, and the transmission belt 428 can be sleeved on the main wheel 426 and the secondary wheel 427 to drive the secondary wheel 427 to rotate with the main wheel 426, thereby driving the main shaft 421 and the secondary shaft 423 to rotate together, i.e., the secondary shaft 423 performs a revolution motion around its own central axis. It can be understood that the revolution of the secondary shaft 423 is driven by the transmission belt 428, and the revolution of the secondary shaft 423 around the main shaft 421 is driven by the connecting rod between the main shaft 421 and the secondary shaft 423. Removing the transmission belt 428 can make the secondary shaft 423 only perform a revolution motion around the main shaft 421 through the connecting rod, and no longer perform a revolution motion.

[0041] The number of the secondary shafts 423, the secondary wheels 427 and the transmission belts 428 can be multiple (two or more), and in the embodiment, two secondary shafts 423, two secondary wheels 427 and two transmission belts 428 are provided. The diameters of the primary wheel 426 and the secondary wheel 427 can be different, so that the rotation speeds of the primary shaft 421 and the secondary shaft 423 are different (in particular, the rotation speed of the secondary shaft can be different from that of the primary shaft). The primary shaft 421 can be arranged on the central axis of the enzymolysis tank 40, or the primary shaft 421 can be arranged close to the central axis of the enzymolysis tank 40 relative to the secondary shaft 423. The multiple secondary shafts 423 can be uniformly distributed along the circumference of the enzymolysis tank 40. The differential stirring device 42 can form the rotation of the primary shaft 421, the revolution of the secondary shaft 423 and the rotation of the secondary shaft 423 in the enzymolysis tank 40 when stirring the casein, which can effectively enhance the shearing force on the casein, slow down the formation of casein micelles, and avoid or reduce the formation of large casein lumps in the enzymolysis tank 40. It can be understood that in a variant, the motor 425 can also be connected to a secondary shaft 423, and the remaining secondary shafts 423 which are not directly connected to the motor 425 can be connected to the primary shaft 421 or the secondary shaft 423 connected to the motor.

[0042] Preferably, the diameter of the primary wheel 426 can be greater than that of the secondary wheel 427. More preferably, the diameter of the primary wheel 426 can be 1.5 to 2.5 times that of the secondary wheel 427.

[0043] It can be understood that the paddle arrangement and type of the primary shaft 421 and the secondary shaft 423 are not particularly limited and can be set according to the actual production needs. The primary wheel 426 and the secondary wheel 427 can be belt wheels, and the transmission belt 428 can be a transmission belt. The primary wheel 426, the secondary wheel 427 and the transmission belt 428 can also be other synchronous belt wheel systems; or the transmission belt 428 can also be replaced by a transmission chain, and the primary wheel 426 and the secondary wheel 427 can also be sprocket wheels, i.e., the transmission chain wheel system is used to replace the above-mentioned belt wheel system.

[0044] Preferably, the differential stirring device 42 can further comprise a scraper 429 connected to the primary shaft 421. The scraper 429 can be close to (abut) the inner wall of the enzymolysis tank 40, and when the primary shaft 421 rotates, the scraper 429 can move along the inner wall of the enzymolysis tank 40, i.e., the scraper 429 can rotate (revolve) around the primary shaft 421 to scrape the material on the inner wall of the enzymolysis tank 40. The scraper 429 can be a strip-shaped scraper, a sheet-shaped scraper and a cylindrical scraper, etc.

[0045] Further, as shown in FIG. 4 and FIG. 5, the enzymolysis tank 40 can be connected with a first external circulation pipeline 61. Two ends of the first external circulation pipeline 61 can be connected to the bottom and the upper part of the enzymolysis tank 40 respectively, so as to extract the material in the enzymolysis tank 40 from the bottom and then enter from the upper part, so as to realize the circulation of the material in the enzymolysis tank 40 in the up-down direction of the enzymolysis tank and outside. The first external circulation pipeline 61 can be provided with a first circulation pump 71, and preferably, the first circulation pump 71 can be a centrifugal pump. It can be understood that the setting of the first external circulation pipeline 61 can further enhance the flowability of the material in the enzymolysis tank 40, which can further slow down the formation of casein micelles in combination with the differential speed stirring device 42 described above, avoid or reduce the formation of larger casein micelles in the enzymolysis tank 40, and at the same time, can also increase the enzymolysis efficiency and reduce the amount of enzyme used.

[0046] As shown in FIG. 5, FIG. 6 and FIG. 9, the first external circulation pipeline 61 can be provided with a pipeline type extrusion impactor 611. The pipeline type extrusion impactor 611 can be arranged in the transverse pipeline (horizontally arranged pipeline) of the first external circulation pipeline 61. The pipeline type extrusion impactor 611 can include a joint 6111, a variable diameter pipe 6112, an extrusion head 6113 and a turbulence groove 6114. The joint 6111 can be arranged at both ends of the pipeline type extrusion impactor 611, used to connect the pipeline type extrusion impactor 611 to the pipeline, and the joint 6111 can be a threaded joint. The variable diameter pipe 6112 can be one or two (exemplarily, two variable diameter pipes 6112 are included in the present embodiment), which can be connected to the joint 6111 and arranged inside the joint 6111 (close to one side of the middle part of the pipeline type extrusion impactor), and at least part of the pipe diameter can be changed (in particular, at least part of the pipe diameter can be uniformly changed), so as to change the pressure of the material flowing through the pipeline type extrusion impactor 611. The extrusion head 6113 can be arranged inside the variable diameter pipe 6112 on the inlet side, which has a smaller pipe diameter, and can further extrude the material, used to break the casein micelles. The turbulence groove 6114 can be arranged in the middle part of the pipeline type extrusion impactor 611, and the turbulence groove 6114 can form an irregular flow path (a flow path with a variable pipe diameter), used to further press the material to break the casein micelles. It can be understood that the casein micelles in the material will be subjected to a larger pressure and shear force when passing through the variable diameter pipe 6112, the extrusion head 6113 and the turbulence groove 6114, which can break the casein micelles, reduce the problems of casein agglomeration, precipitation and wall sticking and the like.

[0047] It can be understood that under the joint action of the differential speed stirring device 42 and the first external circulation pipeline 61, the material in the enzymolysis tank 40 can flow sufficiently, improve the action probability of the enzyme and the substrate, and increase the efficiency of protease hydrolysis. That is, under the same enzymolysis time and other conditions, the amount of enzyme required by the enzymolysis tank 40 provided by the present embodiment is less.

[0048] As shown in Fig. 4, the enzymatic tank 40 can also be connected with a second external circulation pipeline 62, which can be provided with an external heat exchanger 50 and a second circulation pump 72. The external heat exchanger 50 can include a heating mode and a cooling mode, which are respectively used to heat or cool the material entering the second circulation pump 72. The second circulation pump 72 can be a centrifugal pump. It can be understood that multiple enzymatic tanks 40 can share part of the second external circulation pipeline 62, including sharing part of the pipeline and sharing one external heat exchanger 50. The two ends of the second external circulation pipeline 62 can be respectively connected to the bottom and the upper part of the enzymatic tank 40, so that the material in the enzymatic tank 40 is extracted from the bottom of the enzymatic tank 40, and then enters the enzymatic tank 40 from the upper part of the enzymatic tank 40 after the temperature of the material is regulated by the external heat exchanger 50. For example, as shown in Fig. 4, three enzymatic tanks 40 can be connected in parallel, and the three enzymatic tanks 40 can be connected to one second external circulation pipeline 62. Preferably, the external heat exchanger 50 can be a tube heat exchanger. It can be understood that the external heat exchanger 50 provided in the second external circulation pipeline 62 can jointly regulate the temperature of the material in the enzymatic tank with the heat exchange jacket 41 of the enzymatic tank 40, especially when the end of the enzymolysis stage enters the enzyme inactivation stage, the external heat exchanger 50 can rapidly increase the temperature of the material in the enzymatic tank 40 with the heat exchange jacket 41. By reducing the temperature transition time from the enzymolysis stage to the enzyme inactivation stage, the stability of the quality of the enzymatic casein polypeptide product can be improved.

[0049] Preferably, as shown in Figs. 5 and 6, the acid-base configuration device 30 (dilute alkali tank 33 and dilute acid tank 34) connected to the pipeline outlet of the enzymatic tank 40 can be provided with a three-fluid atomizing nozzle 80. As shown in Figs. 10 and 11, the three-fluid atomizing nozzle 80 has a triple sleeve structure, which can include an air inner tube 81, an acid-base liquid tube 82 and an air outer tube 83. The air inner tube 81 can be arranged at the innermost side of the three-fluid atomizing nozzle 80 (i.e., the radial inner side of the acid-base liquid tube), and the air outer tube 83 can be arranged at the outermost side of the three-fluid atomizing nozzle 80 (i.e., the radial outer side of the acid-base liquid tube), both of which can be connected to compressed gas. The acid-base liquid tube 82 can be arranged in the middle layer of the three-fluid atomizing nozzle 80, which is used to connect to acid or alkali liquid. The end of the outlet of the air inner tube 81 can be provided with a cyclone sheet 84 to form a cyclone at the outlet of the air inner tube 81. Under the joint action of the compressed gas of the air inner tube 81 and the air outer tube 83, the acid or alkali liquid at the outlet of the acid-base liquid tube 82 can be rapidly atomized into fine droplets and mixed with the material in the enzymatic tank 40, and the distribution of the acid-base liquid droplets is uniform, which can avoid the local over-high or over-low pH of the material to denature the protein. It can be understood that the three-fluid atomizing nozzle 80 can be connected to a compressed gas pipeline or a compressed gas device. Here, the compressed gas can be compressed air (the oxygen in the air has little effect on the reaction in the enzymatic tank), and the compressed gas can also be inert gas such as nitrogen.

[0050] The preparation system can further comprise a cleaning-in-place (CIP) unit for cleaning the devices and pipelines of the preparation system.

[0051] The embodiments of the present application also provide a control method of the industrial preparation system of the above-mentioned enzymatic casein polypeptide.

[0052] Firstly, a casein solution with a solid content of 10%-20% can be selected and subjected to pasteurization by the sterilization device 10. The sterilization temperature can be 75-85°C, and the sterilization holding time can be 15-30s. The material after pasteurization can be cooled to 4-6°C and then temporarily stored in the enzyme hydrolysis tank 40.

[0053] The enzyme preparation is accurately weighed and added into the enzyme addition tank 20, and RO water is added to fully dissolve the enzyme preparation.

[0054] The nitric acid solution, the food-grade sodium hydroxide solid, and the concentrated acid tank 31 and the concentrated alkali tank 32 are weighed and added, respectively, and after fully dissolved by stirring, the acid solution and the alkali solution are pumped into the dilute acid tank 34 and the dilute alkali tank 33, respectively, for accurate preparation.

[0055] The heat exchange jacket of the enzyme hydrolysis tank 40 is started to heat and warm the material in the enzyme hydrolysis tank 40. The saturated steam pressure of the steam entering the heat exchange jacket 41 can be 0.2-0.5Mpa, and the saturated steam temperature can be 132-158°C. The material is warmed from 4-6°C to 40-60°C, so that the material is at a suitable enzyme hydrolysis temperature, and the warming time can be less than or equal to 30min.

[0056] During the above-mentioned warming process, the differential speed stirring device 42 can be started, the main shaft 421 of the differential speed stirring device 42 can rotate at a speed of 10-30r / min, and the secondary shaft 423 can rotate at a speed of 15-75r / min. At the same time, the first circulating pump 71 of the first external circulation pipeline 61 can be started to make the material continuously flow through the first external circulation pipeline.

[0057] The pH control range of the enzyme hydrolysis liquid is set, the compressed gas valve connected to the three-fluid atomizing nozzle 80 is opened, and compressed gas is introduced into the air inner pipe 81 and the air outer pipe 83, and the pressure of the compressed gas can be 0.1-0.6Mpa. The accurately prepared acid solution or alkali solution is introduced into the acid-alkali liquid pipe 82, so that the acid solution or alkali solution is rapidly atomized into small droplets of 0.03-0.1μm under the action of the inner and outer compressed gas and the cyclone plate 84 and uniformly dispersed in the material.

[0058] During the enzymatic hydrolysis process, the first circulation pump 71 is continuously turned on, and the material can be continuously circulated through the first external circulation pipeline 61. During the circulation process, the material flowing through the pipeline type extrusion impactor 611 can break the casein micelles. The differential speed stirring device 42 of the enzymatic hydrolysis tank 40 is operated at least partially simultaneously with the first external circulation pipeline 61 for a period of time, so that the material in the enzymatic hydrolysis tank 40 is fully flowed.

[0059] After the enzymatic hydrolysis process is completed, the first circulation pump 71 is turned off, the steam pressure of the heat exchange jacket 41 is increased, the second circulation pump 72 and the heating mode of the external heat exchanger 50 are started, and the enzymatic hydrolysis liquid in the enzymatic hydrolysis tank 40 is heated by the "double heating" mode of the steam heating of the heat exchange jacket 41 and the simultaneous heating of the external heat exchanger 50. The temperature of 1000-5000 kg (kilograms) of material can be increased from 40-60°C to 85-95°C in 2-6 min. It can be understood that if the enzyme is not inactivated after the enzymatic hydrolysis process, the polypeptides formed after the casein hydrolysis may be further hydrolyzed, which affects the quality of the product.

[0060] After the enzyme inactivation temperature is reached, the second circulation pump 72 and the heating mode of the external heat exchanger 50 can be turned off, and the steam pressure of the heat exchange jacket 42 can be reduced, while the differential speed stirring device 42 can continue to operate.

[0061] After the enzyme inactivation process is completed, the second circulation pump 72 and the cooling mode of the external heat exchanger 50 can be started, and the material can be cooled to 4-6°C or 40-45°C according to the requirements of the subsequent production process, and then poured into the storage tank for standby.

[0062] Optionally, the method for controlling the pH of the enzymatic hydrolysis liquid can be continuously adjusted to maintain a constant pH during the enzymatic hydrolysis process, or it can be adjusted once before the enzymatic hydrolysis process.

[0063] Optionally, the enzyme inactivation process after the enzymatic hydrolysis process, such as the mass of the material is in the range of 1000-2000 kg, can also use only the steam heating of the heat exchange jacket 41.

[0064] Three embodiments of specific parameter settings under the above control method are given below.

[0065] First embodiment

[0066] The casein solution with a solid content of 15% is selected, the sterilization temperature is 80°C, and the sterilization holding time is 24s;

[0067] During the enzymatic hydrolysis temperature increasing process, the saturated steam pressure of the heat exchange jacket 41 for heating the material is 0.3Mpa, the saturated steam temperature is 142°C, and the material is heated from 4-6°C to 60°C for enzymatic hydrolysis. The temperature increasing time is 27.3 min;

[0068] The main shaft 421 rotates at 20 r / min, and the secondary shaft 423 rotates at 40 r / min.

[0069] The compressed gas pressure is 0.3 Mpa.

[0070] During the enzyme inactivation process, 3000 kg of material is heated from the enzyme hydrolysis temperature of 60℃ to the enzyme inactivation temperature of 90℃, and the time consumption is 4.2 min.

[0071] Second embodiment

[0072] The casein solution with a solid content of 10% is selected, the sterilization temperature is 75℃, and the sterilization holding time is 30 s.

[0073] During the enzyme hydrolysis heating process, the saturated steam pressure of the heat exchange jacket 41 for heating the material is 0.2 Mpa, and the saturated steam temperature is 132℃. The material is heated from 4-6℃ to the enzyme hydrolysis temperature of 40℃, and the heating time is 24.6 min.

[0074] The main shaft 421 rotates at 10 r / min, and the secondary shaft 423 rotates at 15 r / min.

[0075] The compressed gas pressure is 0.1 Mpa.

[0076] During the enzyme inactivation process, 1000 kg of material is heated from the enzyme hydrolysis temperature of 40℃ to the enzyme inactivation temperature of 85℃, and the time consumption is 2.3 min.

[0077] Third embodiment

[0078] The casein solution with a solid content of 20% is selected, the sterilization temperature is 85℃, and the sterilization holding time is 15 s.

[0079] During the enzyme hydrolysis heating process, the saturated steam pressure of the heat exchange jacket 41 for heating the material is 0.5 Mpa, and the saturated steam temperature is 158℃. The material is heated from 4-6℃ to the enzyme hydrolysis temperature of 60℃, and the heating time is 29.6 min.

[0080] The main shaft 421 rotates at 30 r / min, and the secondary shaft 423 rotates at 75 r / min.

[0081] The compressed gas pressure is 0.6 Mpa.

[0082] During the enzyme inactivation process, 5000 kg of material is heated from the enzyme hydrolysis temperature of 60℃ to the enzyme inactivation temperature of 95℃, and the time consumption is 5.6 min.

[0083] It should be understood that some aspects of the above embodiments can be appropriately combined.

[0084] The advantages of the above technical solutions compared with the prior art are illustrated below with examples and comparative examples.

[0085] 1. Comparison of material state after enzymatic process and enzyme inactivation

[0086] In the above Table 1, it can be seen that the material state of each embodiment of the present application is better than the technical solutions in the above comparative examples.

[0087] Table 1: Comparison of material state of each embodiment and comparative examples one and two

[0088] In the above Table 1, it can be seen that the material state of each embodiment of the present application is better than the technical solutions in the above comparative examples.

[0089] 2. Comparison of system heating efficiency

[0090] Table 2: Comparison of heating efficiency of the first embodiment and comparative examples three, four and five

[0091] In the above Table 2, it can be seen that the heating speed of the first embodiment of the present application is faster than the heating speed of the above comparative examples.

[0092] The following briefly describes some beneficial effects of the above embodiments of the present application.

[0093] 1. The enzymatic casein polypeptide industrial preparation system provided by the embodiment adopts a differential stirring device in the enzyme hydrolysis tank, which greatly enhances the shearing force on casein, slows down the formation of casein micelles, and eliminates or reduces the formation of larger casein micelles in the enzyme hydrolysis tank.

[0094] 2. The enzymatic casein polypeptide industrial preparation system provided by the embodiment adopts a pipeline type extrusion impactor, which can be installed on the material external circulation horizontal circulation pipeline during the enzyme hydrolysis process to fully break the formed casein micelles.

[0095] 3. The pH adjustment of the enzymatic casein polypeptide industrial preparation system provided by the embodiment is more moderate. Through the three-fluid atomizing nozzle, the acid and alkali liquid can form small droplets of 0.05-0.1 μm, which are uniformly distributed in the liquid, avoiding the formation of a local pH that is too high or too low in the solution system, causing casein denaturation and precipitation.

[0096] 4. The enzymatic casein polypeptide industrial preparation system provided by the embodiment has high enzyme hydrolysis reaction efficiency. The technical solution adopts a double circulation mode of tank stirring paddle disturbance plus external circulation to make the liquid flow fully, increase the probability of enzyme and substrate interaction, and improve the enzyme hydrolysis efficiency. Under the same enzyme hydrolysis time conditions, the amount of enzyme can be reduced.

[0097] 5、The enzymatic casein polypeptide industrial preparation system provided by the embodiment has a fast heating rate, adopts a "double heating" mode of heat exchange jacket steam heating and external heat exchanger heating. Under experimental conditions, it only takes 5-6 minutes to raise the temperature of 5000 kg of material in a single tank from the enzymolysis temperature of 60 DEG C to the enzyme inactivation temperature of 90 DEG C, greatly increasing the batch-to-batch stability of the mild and moderate enzymolysis casein polypeptide product.

[0098] It can be understood that, in the present application, the number of components or members is one or more when not particularly limited, and the plurality herein refers to two or more. For the case where the number of components or members is a specific number such as two, three, four, etc. shown in the drawings and / or described in the specification, the specific number is generally exemplary rather than limiting, and can be understood as a plurality, i.e. two or more, but this does not mean that the present application excludes the case of one.

[0099] It should be understood that the above embodiments are only exemplary and are not used to limit the present application. Those skilled in the art can make various modifications and changes to the above embodiments under the teaching of the present application without departing from the scope of the present application.

Claims

1. An industrial system for the preparation of casein polypeptides by enzymatic hydrolysis, characterized in that, The system comprises one or more enzymatic hydrolysis tanks (40) and one or more first external circulation pipelines (61), the enzymatic hydrolysis tank (40) comprises a heat exchange jacket (41) arranged on the wall of the enzymatic hydrolysis tank (40) for regulating the temperature of the material in the enzymatic hydrolysis tank (40), The differential speed stirring device (42) comprises a main shaft (421), a plurality of main paddles (422), a plurality of auxiliary shafts (423), a plurality of auxiliary paddles (424) and a motor (425), The plurality of main paddles (422) are connected to the main shaft (421), the plurality of auxiliary paddles (424) are connected to the auxiliary shafts (423); the motor (425) is connected to the main shaft (421) or one of the auxiliary shafts (423), at least one of the auxiliary shafts (423) is in driving connection with the main shaft (421), so that one of the motors drives the main shaft and the auxiliary shafts (423) to rotate, The rotation speed of the main shaft (421) is different from that of the auxiliary shafts (423) to form differential speed rotation for slowing down the formation of casein micelles, The first external circulation pipeline (61) is connected to the bottom and the upper part of the enzymatic hydrolysis tank (40) respectively, and is provided with a first circulation pump (71) to draw the material in the enzymatic hydrolysis tank (40) from the bottom and then introduce it into the enzymatic hydrolysis tank (40) from the upper part, forming circulation of the material in the enzymatic hydrolysis tank (40) in the upward and downward directions and externally to improve the enzymatic hydrolysis efficiency.

2. The industrial preparation system of enzymatic casein polypeptide according to claim 1, characterized in that, The motor (425) is connected to the main shaft (421), The auxiliary shafts (423) are connected to the main shaft (421) through connecting rods, so that the auxiliary shafts (423) can rotate around the main shaft (421), The differential speed stirring device (42) comprises a main wheel (426), an auxiliary wheel (427) and a transmission belt (428) or a transmission chain, The main wheel (426) is connected to the main shaft (421), the auxiliary wheel (427) is connected to the auxiliary shafts (423), and the transmission belt (428) or the transmission chain is sleeved on the main wheel (426) and the auxiliary wheel (427), so that the auxiliary wheel (427) rotates with the rotation of the main wheel (426), The diameter of the main wheel (426) is greater than that of the auxiliary wheel (427), so that the rotation speed of the main shaft (421) is less than that of the auxiliary shafts (423), and the main shaft (421) is arranged closer to the central axis of the enzymatic hydrolysis tank (40) than the auxiliary shafts (423).

3. The industrial preparation system for enzymatic hydrolysis of casein polypeptides according to claim 1 or 2, characterized in that The differential speed stirring device (42) further comprises a scraper (429) connected to the main shaft (421) and rotating with the main shaft (421), and the scraper (429) abuts against the inner wall of the enzymatic hydrolysis tank (40) to clean the material on the inner wall of the enzymatic hydrolysis tank (40).

4. The industrial preparation system of enzymatic casein polypeptide according to any one of claims 1 to 3, characterized in that, The first outer circulation pipeline (61) is also provided with a pipeline extrusion impactor (611), The pipeline extrusion impactor (611) comprises a joint (6111), a variable diameter pipe (6112), an extrusion head (6113) and a turbulence groove (6114), The joint (6111) is arranged at both ends of the pipeline extrusion impactor (611) to connect the pipeline, the variable diameter pipe (6112) is arranged inside the joint (6111), and at least part of the variable diameter pipe (6112) is variable in diameter, The extrusion head (6113) is arranged inside the variable diameter pipe (6112) and has a nozzle capable of extruding the material, and the turbulence groove (6114) is arranged in the middle of the pipeline extrusion impactor (611).

5. The industrial preparation system of enzymatic casein polypeptide according to any one of claims 1 to 4, characterized in that, A second outer circulation pipeline (62) is also included, which is provided with a second circulation pump (72) and an external heat exchanger (50), Both ends of the second outer circulation pipeline (62) are connected to the bottom and the upper part of the enzymatic tank (40), respectively, so that the material in the enzymatic tank (40) is extracted from the bottom of the enzymatic tank (40), then the temperature of the material is adjusted by the external heat exchanger (50), and finally the material is introduced into the enzymatic tank (40) from the upper part of the enzymatic tank (40).

6. The industrial preparation system of enzymatic casein polypeptide according to claim 5, characterized in that, A plurality of enzymatic tanks (40) and a plurality of first outer circulation pipelines (61) are included, and the plurality of first outer circulation pipelines (61) are each connected to one of the plurality of enzymatic tanks (40), The plurality of enzymatic tanks (40) are connected in parallel, and each of the plurality of enzymatic tanks (40) is connected to the second outer circulation pipeline (62).

7. The industrial preparation system of enzymatic casein polypeptide according to any one of claims 1 to 6, characterized in that, An acid-base configuration device (30) is also included, which is connected to the enzymatic tank (40), The acid-base configuration device (30) is connected to the pipeline end of the enzymatic tank (40), and a three-fluid atomizing nozzle (80) is arranged at the pipeline end, The three-fluid atomizing nozzle (80) comprises an air inner pipe (81), an acid-base liquid pipe (82) and an air outer pipe (83), the air inner pipe (81) is located radially inside the acid-base liquid pipe (82), the acid-base liquid pipe (82) is located radially inside the air outer pipe (83), and the end of the air inner pipe (81) is provided with a cyclone vane (84) to form a gas cyclone, The acid-base liquid pipe (82) can introduce acid or alkali, and the air inner pipe (81) and the air outer pipe (83) can introduce air, so that the acid or alkali can form atomized droplets under the action of the gas flow.

8. The industrial preparation system of enzymatic casein polypeptide according to any one of claims 1 to 7, characterized in that, A sterilization device (10) and one or more enzyme addition tanks (20) are also included, The sterilization device (10) is connected to the enzymatic tank (40) to introduce a sterilized casein solution into the enzymatic tank (40), The enzyme addition tank (20) is connected to the enzymatic tank (40) to introduce an enzyme preparation into the enzymatic tank (40).

9. A control method of an industrial production system of casein polypeptide by enzymatic hydrolysis, characterized by, The enzyme hydrolysis casein polypeptide industrial preparation system of any one of claims 1 to 8, In the enzymatic hydrolysis process of casein, the differential stirring device (42) of the enzyme hydrolysis tank (40) is at least partially operated simultaneously with the first external circulation pipeline (61) to make the material in the enzyme hydrolysis tank (40) flow.

10. A control method of an industrial production system of casein polypeptide by enzymatic hydrolysis, characterized by, The enzyme hydrolysis casein polypeptide industrial preparation system of claim 5 or 6, In the enzyme inactivation process of casein, the heat exchange jacket (41) of the enzyme hydrolysis tank (40) is operated simultaneously with the second external circulation pipeline (62) to make the material in the enzyme hydrolysis tank (40) warm up.

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