A kind of compound and preparation method thereof and method for preparing polypeptide using the same
A technology of compounds and mixtures, which is applied in the field of compounds and their preparation, can solve problems such as polypeptides that need to be improved, and achieve the effect of reducing side reactions and improving reaction efficiency
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[0118] Step 1, synthesis of N-(9-fluorenylmethoxycarbonyl)-S-trifluoroacetylaminomethyl-L-cysteine (Fmoc-Cys(Tfacm)-OH, namely figure 1 compounds shown)
[0119] 1.1, preparation of N-(hydroxymethyl) trifluoroacetamide:
[0120] Put trifluoroacetamide (1.13 g, 10 mmol, 1 eq) and potassium carbonate (138 mg, 1 mmol, 0.1 eq) in a 25 ml round bottom flask, add 0.8 ml of 37% aqueous formaldehyde solution, and react at room temperature for 6 hours, spin dry and set aside;
[0121] 1.2. Preparation of Fmoc-L-cysteine:
[0122] Put fluorenylmethoxycarbonyl-S-trityl-L-cysteine (5.85 g, 10 mmol, 1 eq) in a 500 ml round bottom flask, add 20 ml trifluoroacetic acid, 20 ml dichloro Methane, 1 ml triphenylsilane mixed solution, react at room temperature for 1 hour, spin dry and wash with petroleum ether to remove triphenylmethane, and spin dry the remaining white solid to collect for later use;
[0123] 1.3. Preparation of N-(9-fluorenylmethoxycarbonyl)-S-trifluoroacetamidomethyl-L...
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