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Application of etonogestrel in the preparation of anti-B-cell lymphoma products

A B-cell and lymphoma technology, applied in the application field of preparing anti-B-cell lymphoma products, can solve the problems of large genetic variation of individual tumors, long cycle, and ineffective anti-tumor drugs.

Active Publication Date: 2019-01-22
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, with the rapid development of tumor genomics and molecular pharmacology, the research and development of new anti-tumor drugs has achieved good results, but due to the large investment in new drug research and development, the bottlenecks such as long cycle cannot be overcome, and the genetic variation of individual tumors is large, etc. characteristics, leading to the ineffectiveness of many traditional antineoplastic drugs, expensive new drugs, and unknown side effects

Method used

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  • Application of etonogestrel in the preparation of anti-B-cell lymphoma products
  • Application of etonogestrel in the preparation of anti-B-cell lymphoma products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1, CELLTITER-GLO detection of Etonogestrel anti-B cell lymphoma

[0046] 1. Preparation of orifice plate to be tested

[0047] 1. Cell plating

[0048] a) Prepare the complete medium required for each cell.

[0049] b) Before the start of the experiment, confirm the drug screening cell name, medium, subculture time, generation and other information marked on the 100mm culture dish to ensure that the experiment is correct.

[0050] c) For the operation of adherent cells, refer to steps d) to i), for the operation of suspension cells, refer to steps j) to l).

[0051] d) Use a vacuum pump to suck up the cell culture medium during aseptic operation.

[0052] e) Rinse the cell surface with 2 ml of sterile PBS solution, and then suck out the PBS waste liquid with a vacuum pump.

[0053] f) Gently add 1ml 0.25% (w / v) Trypsin-0.038% (w / v) EDTA solution to the culture dish to digest the cells, mix gently for a few times, the solution covers the cell surface, and obs...

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Abstract

A use of etonogestrel in the preparation of anti-B cell lymphoma products.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an application of Etonogestrel in the preparation of anti-B cell lymphoma products. Background technique [0002] Tumor is the most common and serious disease that threatens human health, and the development of highly effective anti-tumor drugs is crucial to prolonging the survival period of patients. In recent years, with the rapid development of tumor genomics and molecular pharmacology, the research and development of new anti-tumor drugs has achieved good results, but due to the large investment in new drug research and development, the bottlenecks such as long cycle cannot be overcome, and the genetic variation of individual tumors is large, etc. Many traditional antineoplastic drugs are ineffective, new drugs are expensive, and side effects are unknown. [0003] Etonogestrel is a subcutaneous implant primarily used for contraception and belongs to the class of steroidal proges...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/567A61P35/00
CPCA61K31/567
Inventor 师咏勇宋智健
Owner SHANGHAI JIAO TONG UNIV
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