Application of etonogestrel in the preparation of anti-B-cell lymphoma products

Abstract

A use of etonogestrel in the preparation of anti-B cell lymphoma products.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to an application of Etonogestrel in the preparation of anti-B cell lymphoma products. Background technique

[0002] Tumor is the most common and serious disease that threatens human health, and the development of highly effective anti-tumor drugs is crucial to prolonging the survival period of patients. In recent years, with the rapid development of tumor genomics and molecular pharmacology, the research and development of new anti-tumor drugs has achieved good results, but due to the large investment in new drug research and development, the bottlenecks such as long cycle cannot be overcome, and the genetic variation of individual tumors is large, etc. Many traditional antineoplastic drugs are ineffective, new drugs are expensive, and side effects are unknown.

[0003] Etonogestrel is a subcutaneous implant primarily used for contraception and belongs to the class of steroidal proges...

Examples

Embodiment 1

[0045] Example 1, CELLTITER-GLO detection of Etonogestrel anti-B cell lymphoma

[0046] 1. Preparation of orifice plate to be tested

[0047] 1. Cell plating

[0048] a) Prepare the complete medium required for each cell.

[0049] b) Before the start of the experiment, confirm the drug screening cell name, medium, subculture time, generation and other information marked on the 100mm culture dish to ensure that the experiment is correct.

[0050] c) For the operation of adherent cells, refer to steps d) to i), for the operation of suspension cells, refer to steps j) to l).

[0051] d) Use a vacuum pump to suck up the cell culture medium during aseptic operation.

[0052] e) Rinse the cell surface with 2 ml of sterile PBS solution, and then suck out the PBS waste liquid with a vacuum pump.

[0053] f) Gently add 1ml 0.25% (w / v) Trypsin-0.038% (w / v) EDTA solution to the culture dish to digest the cells, mix gently for a few times, the solution covers the cell surface, and obs...

technical field

[0001] The invention relates to the field of biotechnology, in particular to an application of Etonogestrel in the preparation of anti-B cell lymphoma products. Background technique

[0002] Tumor is the most common and serious disease that threatens human health, and the development of highly effective anti-tumor drugs is crucial to prolonging the survival period of patients. In recent years, with the rapid development of tumor genomics and molecular pharmacology, the research and development of new anti-tumor drugs has achieved good results, but due to the large investment in new drug research and development, the bottlenecks such as long cycle cannot be overcome, and the genetic variation of individual tumors is large, etc. Many traditional antineoplastic drugs are ineffective, new drugs are expensive, and side effects are unknown.

[0003] Etonogestrel is a subcutaneous implant primarily used for contraception and belongs to the class of steroidal proges...

Examples

Embodiment 1

[0045] Example 1, CELLTITER-GLO detection of Etonogestrel anti-B cell lymphoma

[0046] 1. Preparation of orifice plate to be tested

[0047] 1. Cell plating

[0048] a) Prepare the complete medium required for each cell.

[0049] b) Before the start of the experiment, confirm the drug screening cell name, medium, subculture time, generation and other information marked on the 100mm culture dish to ensure that the experiment is correct.

[0050] c) For the operation of adherent cells, refer to steps d) to i), for the operation of suspension cells, refer to steps j) to l).

[0051] d) Use a vacuum pump to suck up the cell culture medium during aseptic operation.

[0052] e) Rinse the cell surface with 2 ml of sterile PBS solution, and then suck out the PBS waste liquid with a vacuum pump.

[0053] f) Gently add 1ml 0.25% (w / v) Trypsin-0.038% (w / v) EDTA solution to the culture dish to digest the cells, mix gently for a few times, the solution covers the cell surface, and obs...

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