Effect-directed target/non-target androgen disruptor identification method

Abstract

The invention discloses an effect-directed target/non-target identification method for androgen disruptors in complicated environment samples. The effect-directed target/non-target identification method includes the steps of pretreatment and primary separation of the samples, androgen activity test, high-throughput separation and preparation of toxic substances, active component scanning based on high-performance liquid chromatography-time-of-flight mass spectrometry, target androgen disruptor screening and non-target androgen disruptor identification and toxicant confirmation by combining mass spectrum, chromatography and toxic characteristics. The effect-directed target/non-target identification method has the advantages that an SPE (solid phase extraction) column and a preparation and separation series method are used for separation, a high-throughput separation and concentration method is optimized by DMSO (dimethyl sulfoxide) serving as a protective agent, target identification of key toxicants is achieved through a target database, and mass spectral characteristic identification based on a high-performance liquid chromatography-time-of-flight mass spectrometry library, chromatographic characteristic identification based on retention time and compound physico-chemical property and toxic characteristic identification based on a molecular dynamics simulation technology are conducted to achieve non-target identification/toxicant confirmation of the key androgen disruptors.

Description

technical field [0001] The present invention relates to an effect-oriented identification method of key androgen interfering substances, more specifically, relates to a screening and qualitative identification of androgen interfering substances based on reporter gene experiment toxicity test and fractional separation of compound polluted environmental samples and methods for quantitative confirmation. Background technique [0002] With the continuous development of social economy, more and more chemical substances are released into the environment in industrial and agricultural production and daily life. Due to the wide variety of pollutants and complex components, environmental pollution is often caused by the interaction of multiple pollutants. Therefore, it is difficult to identify the pollutants in compound polluted environmental samples one by one by relying on the traditional single chemical analysis method, and it will consume a lot of resources. Time and manpower, i...

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problems of extraction, separation, screening and identification of key endocrine disruptors in complex polluted environmental samples that people are increasingly concerned about, the present invention provides a method for the identification of main androgen disruptors in complex polluted environmental samples that is oriented to the effect of androgen activity. The identification method, the invention includes the extraction of environmental samples, high-throughput separation and preparation, qualitative identification, screening and confirmation of high-resolution mass spectrometry combining targeted and non-targeted, so as to identify key androgen interference in compound polluted environmental samples Substances provide a scientific basis for the risk assessment of complex polluted environments

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