Effect-directed target/non-target androgen disruptor identification method

An androgenic, non-targeted technology, applied in biochemical equipment and methods, separation methods, chemical instruments and methods, etc., to reduce sample complexity, optimize high throughput, and reduce complexity

CN105891365AActive Publication Date: 2016-08-24NANJING UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2016-08-24

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Abstract

The invention discloses an effect-directed target / non-target identification method for androgen disruptors in complicated environment samples. The effect-directed target / non-target identification method includes the steps of pretreatment and primary separation of the samples, androgen activity test, high-throughput separation and preparation of toxic substances, active component scanning based on high-performance liquid chromatography-time-of-flight mass spectrometry, target androgen disruptor screening and non-target androgen disruptor identification and toxicant confirmation by combining mass spectrum, chromatography and toxic characteristics. The effect-directed target / non-target identification method has the advantages that an SPE (solid phase extraction) column and a preparation and separation series method are used for separation, a high-throughput separation and concentration method is optimized by DMSO (dimethyl sulfoxide) serving as a protective agent, target identification of key toxicants is achieved through a target database, and mass spectral characteristic identification based on a high-performance liquid chromatography-time-of-flight mass spectrometry library, chromatographic characteristic identification based on retention time and compound physico-chemical property and toxic characteristic identification based on a molecular dynamics simulation technology are conducted to achieve non-target identification / toxicant confirmation of the key androgen disruptors.
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Description

technical field

[0001] The present invention relates to an effect-oriented identification method of key androgen interfering substances, more specifically, relates to a screening and qualitative identification of androgen interfering substances based on reporter gene experiment toxicity test and fractional separation of compound polluted environmental samples and methods for quantitative confirmation. Background technique

[0002] With the continuous development of social economy, more and more chemical substances are released into the environment in industrial and agricultural production and daily life. Due to the wide variety of pollutants and complex components, environmental pollution is often caused by the interaction of multiple pollutants. Therefore, it is difficult to identify the pollutants in compound polluted environmental samples one by one by relying on the traditional single chemical analysis method, and it will consume a lot of resources. Time and manpower, i...

Examples

Embodiment 1

[0048] An effect-oriented target / non-target androgen disruptor identification method in this embodiment, the steps are:

[0049] (1) Sample extraction: The accelerated solvent extraction device Dionex ASE350 is used to extract the organic matter in the soil. During the extraction process, the mixed solvent of dichloromethane and n-hexane (1:1, v:v) is used for 3 rounds of extraction and collected. , and then extracted with pure methanol solvent for 3 rounds and collected separately.

[0050] (2) Solid-phase extraction: After the solid-phase extraction column is activated with 10mL of n-hexane, 10mL of dichloromethane and 10mL of methanol in advance, the extract from the accelerated solvent extraction is enriched and purified through the solid-phase extraction column, The flow rate is controlled at 1-2 drops / second.

[0051] (3) Elution and concentration: use 10mL of methanol:dichloromethane=1:1 mixed solution, 10mL of dichloromethane:n-hexane=4:1 mixed solution and 10mL of n-...