Sea purslane phytoene dehydrogenase gene and construction of VIGS silencing system

Abstract

The invention discloses construction of a phytoene dehydrogenase gene and a VIGS silencing system, the VIGS silencing system of the phytoene dehydrogenase gene is constructed through a specific fragment, and the silencing system can effectively reduce the expression level of SpPDS gene in the sea horse, can realize virus-induced related gene silencing traits, and has a good application prospect. Meanwhile, the VIGS system constructed by the invention has good stability, can be used for verifying the function of the gene in the sedum lineare, and lays a foundation for excavating the sedum lineare gene with a stress resistance function and cultivating plant varieties with excellent soil remediation and saline-alkali soil transformation potential.

Description

technical field

[0001] The invention belongs to the field of genetic engineering, in particular to the construction of a hippocampal phytoene dehydrogenase gene and a VIGS silencing system. Background technique

[0002] Sesuvium portulacastrum, also known as seaside vegetable, belongs to the dicotyledonous plant class Caryophyllaceae, and is a succulent herbaceous halophyte that grows close to the tropical and subtropical coastal sandy land. Found on sandy beaches on tropical and subtropical coasts. In China, hippocampal teeth are mainly distributed in the coastal areas of Hainan, Guangdong, Fujian, Penghu Islands and central and southern Taiwan. As a member of the mangrove wetland ecosystem, hippocampus is an associated plant of mangroves. It reproduces rapidly and exhibits the natural characteristics of salt and alkali resistance, drought resistance, strong light and heavy metal resistance in various adversity environments. Therefore, it can be used as a Potential plants...

Examples

Embodiment 1

[0023] Embodiment 1: the cloning of hippocampus SpPDS gene-specific target fragment and the construction of expression vector

[0024] 1. Obtaining the full-length gene sequence of SpPDS

[0025] The Hippocampus plant material was collected from the vicinity of Hongsha Wharf, Jiyang District, Sanya City, Hainan Province. The experimental material was planted in the State Key Laboratory of Life Sciences, Hainan Normal University, and cultivated under normal outdoor conditions. Whole plants including roots, stems, leaves, flowers and other vegetative organs were selected as materials and used PacBio third-generation sequencing technology for transcriptome sequencing. The transcriptome sequencing results were searched and confirmed by bioinformatics tools such as local blast. The full-length gene sequence of the hippocampal phytoene dehydrogenase SpPDS gene cDNA was obtained, and the full-length nucleotide sequence thereof is shown in SEQ ID NO.1.

[0026] 2. SpPDS gene-specific...

technical field

[0001] The invention belongs to the field of genetic engineering, in particular to the construction of a hippocampal phytoene dehydrogenase gene and a VIGS silencing system. Background technique

[0002] Sesuvium portulacastrum, also known as seaside vegetable, belongs to the dicotyledonous plant class Caryophyllaceae, and is a succulent herbaceous halophyte that grows close to the tropical and subtropical coastal sandy land. Found on sandy beaches on tropical and subtropical coasts. In China, hippocampal teeth are mainly distributed in the coastal areas of Hainan, Guangdong, Fujian, Penghu Islands and central and southern Taiwan. As a member of the mangrove wetland ecosystem, hippocampus is an associated plant of mangroves. It reproduces rapidly and exhibits the natural characteristics of salt and alkali resistance, drought resistance, strong light and heavy metal resistance in various adversity environments. Therefore, it can be used as a Potential plants...

Examples

Embodiment 1

[0023] Embodiment 1: the cloning of hippocampus SpPDS gene-specific target fragment and the construction of expression vector

[0024] 1. Obtaining the full-length gene sequence of SpPDS

[0025] The Hippocampus plant material was collected from the vicinity of Hongsha Wharf, Jiyang District, Sanya City, Hainan Province. The experimental material was planted in the State Key Laboratory of Life Sciences, Hainan Normal University, and cultivated under normal outdoor conditions. Whole plants including roots, stems, leaves, flowers and other vegetative organs were selected as materials and used PacBio third-generation sequencing technology for transcriptome sequencing. The transcriptome sequencing results were searched and confirmed by bioinformatics tools such as local blast. The full-length gene sequence of the hippocampal phytoene dehydrogenase SpPDS gene cDNA was obtained, and the full-length nucleotide sequence thereof is shown in SEQ ID NO.1.

[0026] 2. SpPDS gene-specific...

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