A Mass Spectrometry Ionization Method Based on Tesla Coil Principle

Abstract

This invention discloses a mass spectrometry ionization method based on the Tesla coil principle. It utilizes a Tesla coil to generate a micro-plasma beam, which serves as the ion source. Liquid samples are desorbed and introduced into the micro-plasma beam region for ionization, or gas samples are directly introduced into the micro-plasma beam region for ionization. This invention, by creatively using a Tesla coil-generated micro-plasma beam as the ion source, not only achieves strong ionization capability and good ionization effect on volatile gas samples and low-polarity or non-polar liquid samples, but also requires sample volumes as low as picoliters to microliters, exhibits good solvent compatibility and strong salt resistance, and further possesses advantages such as simple operation, low cost, ease of implementation, and broad applicability.

Description

Technical Field

[0001] This invention relates to a mass spectrometry ionization method based on the principle of Tesla coils, belonging to the field of mass spectrometry analysis technology. Background Technology

[0002] Plasma is the fourth state of matter, in addition to solid, liquid, and gas. Since the term "plasma" was first coined by American chemist and physicist Irving Langmuir in 1928, plasma-based technologies have been applied to daily life and production for over a century, and in-depth research into its fundamental principles and properties has remained a hot topic in scientific research. In the field of mass spectrometry, the property of plasma's numerous active species (excited-state atoms, molecules, ions, etc.) to ionize compounds is of great value in the development of ion sources. Therefore, a series of atmospheric pressure ionization technologies based on plasma principles have been reported, such as Direct Artificial Ion Source (DART), Flow Afterglow Atmospheric Pressure Glow Discharge Ion Source (FA-APGD), Dielectric Barrier Discharge Ionization Source (DBDI), Low Temperature Plasma Ion Source (LTP), Atmospheric Pressure Flame Ion Source (AFI), and Carbon Fiber Ion Source (CFI), etc. These technologies typically involve the generation of active plasma species caused by factors such as discharge, illumination, or high heat, utilizing their energy to achieve the desorption and further ionization of target compounds. Furthermore, these atmospheric pressure ionization sources do not require sample pretreatment when used for sample analysis, can achieve a solvent-free process, have high sensitivity and high test throughput, and can simultaneously obtain online rapid detection of different types of organic molecules in the sample.

[0003] Although a wide variety of plasma-based ion sources have been developed, most of them are good for surface analysis and produce plasma beams that are too large to achieve good ionization analysis results for small samples.

[0004] A Tesla coil, also known as a Tesla coil, consists of an induction coil, a transformer, a igniter, two large capacitors, and a transformer with only a few turns of the primary coil. Its principle is to use a transformer to boost ordinary voltage, and then discharge it from the discharge terminal through the two pole coils. Currently, it is mainly used in games or art. To date, there have been no reports on mass spectrometry ionization technology based on the Tesla coil principle. Summary of the Invention

[0005] In view of the above-mentioned problems in the existing technology, the purpose of this invention is to provide a mass spectrometry ionization method based on the Tesla coil principle.

[0006] To achieve the above-mentioned objectives, the present invention adopts the following technical solution:

[0007] A mass spectrometry ionization method based on the Tesla coil principle involves generating a micro-plasma beam using a Tesla coil. This micro-plasma beam serves as the ion source, causing liquid samples to be desorbed and introduced into the micro-plasma beam region for ionization, or causing gas samples to be introduced into the micro-plasma beam region for direct ionization.

[0008] One embodiment of the mass spectrometry ionization method based on the Tesla coil principle includes the following operations: introducing a liquid sample or a gas sample through a sample introduction device, the outlet end of which is located in front of the mass spectrometer inlet port, wherein the sample introduction device for introducing the liquid sample has a desorption function; generating a micro-plasma beam using a Tesla coil, and positioning the outlet end of the sample introduction device within or around the micro-plasma beam generated by the Tesla coil, so that the liquid sample is desorbed and introduced into the micro-plasma beam region for ionization, or the gas sample is introduced into the micro-plasma beam region for direct ionization.

[0009] In one embodiment, the temperature of the miniature plasma beam generated by the Tesla coil is 20–40°C.

[0010] In one embodiment, the volume of the miniature plasma beam generated by the Tesla coil is 0.5 to 1 cubic millimeter.

[0011] In one embodiment, when the sample is a liquid sample, the sample introduction device is an electrospray needle, an atomizing plate, or a sample introduction capillary with a metal coating on its inner wall.

[0012] In a preferred embodiment, when the sample is a gaseous sample, the sample introduction device is a reagent bottle.

[0013] In one embodiment, the distance between the outlet end of the sample introduction device and the port of the mass spectrometer injection port is 3-5 mm.

[0014] In one embodiment, the distance between the central axis of the micro plasma beam generated by the Tesla coil and the exit end of the sample introduction device is 1 to 3 mm.

[0015] In one embodiment, the distance between the central axis of the miniature plasma beam generated by the Tesla coil and the port of the mass spectrometer inlet is 3-5 mm.

[0016] Compared with the prior art, the beneficial technical effects of the present invention are as follows:

[0017] This invention creatively uses a miniature plasma beam generated by a Tesla coil as an ion source, achieving mass spectrometry ionization analysis with strong ionization capability and good ionization effect on volatile gas samples and low polarity or non-polar liquid samples. The required sample volume can be as low as picoliter to microliter, with good solvent compatibility and strong salt resistance. In addition, it has the advantages of simple operation, low cost, ease of implementation, and strong universality. Therefore, this invention represents a significant advancement compared to the prior art. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the principle of analyzing liquid samples using the mass spectrometry ionization method based on the Tesla coil principle described in this invention, wherein the sample introduction device is an injection capillary.

[0019] Figure 2 This is a schematic diagram of the principle of mass spectrometry ionization method based on Tesla coil principle described in this invention for analyzing volatile samples, wherein the sample introduction device is a reagent bottle;

[0020] Figure 3 This is the mass spectrum of limonene obtained in Example 1 using the mass spectrometry ionization method based on the Tesla coil principle described in this invention;

[0021] Figure 4 This is the mass spectrum of n-decane obtained by the mass spectrometry ionization method based on the Tesla coil principle described in this invention in Example 2 of this invention;

[0022] Figure 5 This is the mass spectrum of dodecaldehyde obtained by the mass spectrometry ionization method based on the Tesla coil principle described in this invention in Embodiment 3 of the present invention;

[0023] Figure 6 This is the mass spectrum of ferrocene obtained by using the mass spectrometry ionization method based on the Tesla coil principle described in this invention in Embodiment 4 of the present invention;

[0024] Figure 7 This is the mass spectrum of cholesterol obtained by the mass spectrometry ionization method based on the Tesla coil principle described in Embodiment 5 of the present invention;

[0025] Figure 8 This is the mass spectrum of caffeine with added sodium ions obtained by using the mass spectrometry ionization method based on the Tesla coil principle described in Embodiment 6 of the present invention.

[0026] Figure 9 This is a mass spectrum of caffeine with added sodium ions obtained by nano-ESI, which is a comparative example.

[0027] The labels in the diagram are as follows: 1. Mass spectrometer inlet; 2. Miniature plasma beam; 3. Tesla coil; 4. Liquid sample; 5. Gas sample; 6. Inlet capillary; 7. Reagent bottle; d1, Distance between the outlet of the inlet capillary and the port of the mass spectrometer inlet; d2, Distance between the central axis of the miniature plasma beam generated by the Tesla coil and the outlet of the inlet capillary; d3, Distance between the central axis of the miniature plasma beam generated by the Tesla coil and the port of the mass spectrometer inlet. Detailed Implementation

[0028] The technical solution of the present invention will be further described in detail and completely below with reference to the accompanying drawings.

[0029] Please see Figure 1 and Figure 2 As shown: An apparatus for implementing a mass spectrometry ionization method based on the Tesla coil principle described in this invention includes a mass spectrometer inlet 1, a Tesla coil 3 for generating a micro plasma beam 2, and a liquid sample 4 (e.g., ...) for introducing the liquid sample. Figure 1 (as shown) or gas sample 5 (e.g.) Figure 2 The sample introduction device (shown) has its outlet end located in front of the mass spectrometer inlet 1, and its outlet end located in or around the micro plasma beam 2 generated by the Tesla coil 3.

[0030] The output end of the Tesla coil 3 is located near the outlet end of the sample introduction device.

[0031] The device is compatible with common mass spectrometers (such as triple quadrupole mass spectrometers, time-of-flight mass spectrometers, ion trap mass spectrometers, etc.) and can also be applied to other mass spectrometry analyses. When used for mass spectrometry analysis, it can be used in conjunction with common mass spectrometers. It has a wide range of applications and strong practicality.

[0032] The method of achieving mass spectrometry ionization using the above-mentioned device is to generate a miniature plasma beam 2 by using a Tesla coil 3 as an ion source, so that the liquid sample 4 is desorbed and introduced into the region of the miniature plasma beam 2 for ionization, or the gas sample 5 is introduced into the region of the miniature plasma beam 2 for direct ionization.

[0033] For details, please see Figure 1 and Figure 2 As shown,

[0034] Liquid sample 4 (e.g.) is introduced through the sample introduction device. Figure 1 (as shown) or gas sample 5 (e.g. Figure 2As shown), the sample introduction device for introducing liquid sample 4 has a desorption function; the Tesla coil 3 generates a micro plasma beam 2 (since the outlet end of the sample introduction device is located in front of the port of the mass spectrometer inlet 1, and the output end of the Tesla coil 3 is located near the outlet end of the sample introduction device, it is equivalent to generating a micro plasma beam 2 region in front of the port of the mass spectrometer inlet 1), so that the liquid sample 4 is desorbed and introduced into the micro plasma beam 2 region for ionization, or the gas sample 5 is introduced into the micro plasma beam 2 region for direct ionization; the generated sample ions enter the mass spectrometer through the mass spectrometer inlet 1, thus realizing the mass spectrometry analysis of liquid sample 4 or gas sample 5.

[0035] In this invention, the temperature of the miniature plasma beam 2 generated by the Tesla coil 3 is 20-40°C. This ensures that volatile gas samples 5 and low-polarity or non-polar liquid samples 4 can be ionized, while avoiding oxidation, degradation, polymerization, etc. caused by excessively high temperatures, thus effectively guaranteeing the ionization effect of the samples.

[0036] In this invention, the volume of the miniature plasma beam 2 generated by the Tesla coil 3 is 0.5 to 1 cubic millimeter.

[0037] In this invention, when the sample introduced by the sample introduction device is a liquid sample 4, the sample introduction device can be an electrospray needle, an atomizing plate, or a sample introduction capillary 6 with a metal coating on its inner wall (e.g., Figure 1 As shown), the preferred sample inlet capillary 6 is a nanoliter electrospray capillary. The electrospray needle desorbs the liquid sample 4 during spraying, the atomizing plate atomizes and desorbs the liquid sample 4, and the sample inlet capillary 6 with a metal coating on its inner wall can form a discharge structure with the micro plasma beam 2 to desorb the liquid sample 4.

[0038] At this time, the process of realizing the mass spectrometry ionization method of the present invention is as follows: a liquid sample 4 is introduced through a sample introduction capillary 6 with a metal coating on its inner wall, and the outlet end of the sample introduction capillary 6 is located in front of the port of the mass spectrometer inlet 1; a miniature plasma beam 2 is generated by the Tesla coil 3, and the generated miniature plasma beam 2 is brought into contact with the outer wall near the outlet end of the sample introduction capillary 6 to form a discharge structure. The liquid sample 4 in the sample introduction capillary 6 is desorbed under the action of the discharge structure. The desorbed liquid sample 4 is introduced into the region of the miniature plasma beam 2 through the outlet end of the sample introduction capillary 6, and is ionized under the action of the miniature plasma beam 2 (the miniature plasma beam 2 is in contact with the outer wall near the outlet end of the sample introduction capillary 6, which is equivalent to the outlet end of the sample introduction capillary 6 being located around the miniature plasma beam 2. Therefore, when the desorbed liquid sample 4 is introduced to the outlet end of the sample introduction capillary 6, it can be ionized under the action of the miniature plasma beam 2).

[0039] Please see again. Figure 2 As shown, when the sample introduced by the sample introduction device is a gas sample 5, the sample introduction device is a reagent bottle 7. In this case, the method for achieving ionization using the mass spectrometry ionization device of the present invention is as follows: open the cap of the reagent bottle 7 containing the gas sample 5, and position the outlet end of the reagent bottle 7 in front of the port of the mass spectrometer inlet 1; generate a micro-plasma beam 2 using the Tesla coil 3, and position the outlet end of the reagent bottle 7 within or around the micro-plasma beam 2; when the gas sample 5 evaporates from the reagent bottle 7 into the region of the micro-plasma beam 2, the gas sample 5 is ionized under the action of the micro-plasma beam 2.

[0040] In this invention, the distance between the outlet end of the sample introduction device and the port of the mass spectrometer injection port 1 is 3-5 mm, for example, as shown below. Figure 1 As shown, the distance d1 between the outlet end of the injection capillary 6 and the port of the mass spectrometer injection port 1 is 3 mm.

[0041] In this invention, the distance d2 between the central axis of the micro plasma beam 2 generated by the Tesla coil 3 and the exit end of the sample introduction device is 1-3 mm, for example, as shown in the figure. Figure 1 As shown, the distance d2 between the central axis of the miniature plasma beam 2 generated by the Tesla coil 3 and the outlet end of the sample introduction capillary 6 is 1 mm.

[0042] In this invention, the distance d3 between the central axis of the miniature plasma beam 2 generated by the Tesla coil 3 (corresponding to the central axis of the output end of the Tesla coil 3) and the port of the mass spectrometer inlet 1 is 3-5 mm. For example, as shown in the figure... Figure 2 As shown, d3 is 3mm.

[0043] The technical effects achievable by the present invention will be further illustrated below with reference to specific application examples.

[0044] Example 1

[0045] use Figure 2 The apparatus shown is coupled with a mass spectrometer (triple quadrupole mass analyzer) for the analysis of volatile small molecule limonene (MW = 136). Perform mass spectrometry analysis:

[0046] Open the cap of reagent bottle 7 containing limonene and position the bottle opening (i.e., the outlet end) in front of the mass spectrometer inlet 1, directly injecting the sample using the gas evaporation method; generate a miniature plasma beam 2 using Tesla coil 3, and maintain a distance of approximately 3 mm between the miniature plasma beam 2 and the mass spectrometer inlet 1; after the limonene gas sample evaporates from reagent bottle 7 into the region of the miniature plasma beam 2, it undergoes ionization under the action of the miniature plasma beam 2. The resulting sample ions enter the mass spectrometer detector through the mass spectrometer inlet 1 for detection and analysis. The analysis results are as follows: Figure 3 As shown.

[0047] Figure 3 This embodiment shows the mass spectrum obtained by analyzing limonene using the Tesla coil-based mass spectrometry ionization method described in this invention. The spectrum shows the protonated molecular ion peak [M+H] representing the limonene molecule. + =137, indicating that the present invention can achieve good ionization effect on low-polarity volatile small molecules, and the detection is convenient and fast.

[0048] Example 2

[0049] use Figure 2 The apparatus shown is coupled with a mass spectrometer (triple quadrupole mass analyzer) for the analysis of volatile small molecule n-decane (MW = 142). Perform mass spectrometry analysis:

[0050] Open the cap of reagent bottle 7 containing n-decane and position the bottle opening in front of the mass spectrometer inlet 1 for direct gas evaporation injection. Generate a miniature plasma beam 2 using Tesla coil 3, ensuring the distance between the miniature plasma beam 2 and the mass spectrometer inlet 1 is approximately 3 mm. After the n-decane gas sample evaporates from reagent bottle 7 into the region of the miniature plasma beam 2, it undergoes ionization under the influence of the beam. The resulting sample ions enter the mass spectrometer detector through the mass spectrometer inlet 1 for detection and analysis. The analysis results are as follows: Figure 4 As shown.

[0051] Figure 4 This embodiment shows the mass spectrum obtained by analyzing n-decane using the Tesla coil-based mass spectrometry ionization method described in this invention. The spectrum shows the signal peak [M+O-3H] representing the n-decane molecule. + =155 indicates that the present invention can achieve good ionization effect on nonpolar hydrocarbon compounds, without producing fragments, and can obtain the ion signal generated by the complete molecule, and the detection is convenient and fast.

[0052] Example 3

[0053] use Figure 2The apparatus shown is coupled with a mass spectrometer (triple quadrupole mass analyzer) for the analysis of volatile small molecule dodecaldehyde (MW = 184). Perform mass spectrometry analysis:

[0054] Open the cap of reagent bottle 7 containing dodecaldehyde and position the bottle opening in front of the mass spectrometer inlet 1 for direct gas evaporation injection. Generate a miniature plasma beam 2 using Tesla coil 3, ensuring the distance between the miniature plasma beam 2 and the mass spectrometer inlet 1 is approximately 3 mm. After the dodecaldehyde gas sample evaporates from reagent bottle 7 into the region of the miniature plasma beam 2, it undergoes ionization under the influence of the beam. The resulting sample ions enter the mass spectrometer detector through the mass spectrometer inlet 1 for detection and analysis. The analysis results are as follows: Figure 5 As shown.

[0055] Figure 5 This embodiment shows the mass spectrum obtained by analyzing dodecanoic acid using the Tesla coil-based mass spectrometry ionization method described in this invention. The spectrum shows the protonated molecular ion peak [M+H] representing the dodecanoic acid molecule. + =185, indicating that the present invention can achieve good ionization effect on low polarity compounds with poor detection performance of ESI ion source, and the detection is convenient and fast.

[0056] Example 4

[0057] use Figure 1 The apparatus shown is coupled with a mass spectrometer (with a triple quadrupole mass analyzer) for the analysis of the nonpolar compound ferrocene (MW = 186). Perform mass spectrometry analysis:

[0058] Ferrocene was prepared into a sample solution of approximately 50 μg / mL using toluene solvent and set aside. Approximately 1 μL of the ferrocene solution was introduced through a nano-electrospray capillary 6 with a metal-coated inner wall, with the outlet end of the capillary 6 positioned in front of the mass spectrometer inlet 1. A miniature plasma beam 2 was generated by a Tesla coil 3 and brought into direct contact with the outer wall of the capillary 6, forming a discharge structure. This discharge structure desorbed the ferrocene solution within the capillary 6. The distance between the contact point and the tip (i.e., the outlet end) of the capillary 6 was approximately 1 mm, while the distance between the tip of the capillary 6 and the mass spectrometer inlet 1 was approximately 3 mm. When the ferrocene solution reached the tip of the capillary 6, it was located within the region of the miniature plasma beam 2. Under the influence of the miniature plasma beam 2, the ferrocene solution was desorbed from the tip of the capillary 6 and ionized. The resulting sample ions entered the mass spectrometer detector through the mass spectrometer inlet 1 for detection and analysis. The analysis results are as follows: Figure 6 As shown.

[0059] Figure 6This is the mass spectrum obtained by analyzing ferrocene using the Tesla coil-based mass spectrometry ionization method described in this invention. The spectrum shows the molecular ion peak M+ = 186, representing the ferrocene molecule. This indicates that the present invention can achieve good ionization of nonpolar compounds with poor ESI ion source detection performance, and it also has good compatibility with toluene, a poor ESI solvent, making the detection process convenient and fast.

[0060] Example 5

[0061] use Figure 1 The apparatus shown is coupled with a mass spectrometer (triple quadrupole mass analyzer) for the analysis of the low-polarity compound cholesterol (MW = 386). Perform mass spectrometry analysis:

[0062] A cholesterol sample solution of approximately 50 μg / mL was prepared using dichloromethane solvent and set aside. Approximately 1 μL of the cholesterol solution was introduced through a nano-electrospray capillary 6 with a metal-coated inner wall, with the outlet end of the capillary 6 positioned in front of the mass spectrometer inlet 1. A miniature plasma beam 2 was generated by a Tesla coil 3 and brought into direct contact with the outer wall of the capillary 6, forming a discharge structure. This discharge structure desorbed the cholesterol solution within the capillary 6. The distance between the contact point and the tip (i.e., the outlet end) of the capillary 6 was approximately 1 mm, while the distance between the tip of the capillary 6 and the mass spectrometer inlet 1 was approximately 3 mm. When the cholesterol solution reached the tip of the capillary 6, it was located in the region of the miniature plasma beam 2. Under the action of the miniature plasma beam 2, the cholesterol solution was desorbed from the tip of the capillary 6 and ionized. The resulting sample ions entered the mass spectrometer detector through the mass spectrometer inlet 1 for detection and analysis. The analysis results are as follows: Figure 7 As shown.

[0063] Figure 7 This embodiment shows the mass spectrometry analysis of cholesterol obtained using the Tesla coil-based mass spectrometry ionization method described in this invention. The spectrum shows the signal peak [M+H-H2O] representing the cholesterol molecule. + =369, indicating that the present invention can achieve good ionization effect on steroidal compounds with poor ESI ion source detection performance, and has good compatibility with dichloromethane, a poor ESI solvent, and the detection process is convenient and fast.

[0064] Example 6

[0065] use Figure 1 The apparatus shown was coupled to a mass spectrometer (triple quadrupole mass analyzer) to analyze a caffeine solution containing sodium ions (MW = 194). Perform mass spectrometry analysis:

[0066] A sample solution of approximately 10 μg / mL caffeine was prepared using methanol as solvent. Ammonium acetate was added to achieve a sodium ion concentration of approximately 100 μg / mL. Approximately 1 μL of the sodium-added caffeine solution was introduced through a nano-electrospray capillary 6 with a metal-coated inner wall, with the outlet of the capillary 6 positioned in front of the mass spectrometer inlet 1. A miniature plasma beam 2 was generated by a Tesla coil 3 and brought into direct contact with the outer wall of the capillary 6 to form a discharge structure. This discharge structure... The caffeine solution is desorbed, with the contact point approximately 1 mm from the tip of capillary 6 (i.e., the outlet end), and the distance between the tip of capillary 6 and the port of mass spectrometer inlet 1 approximately 3 mm. When the caffeine solution containing sodium ions is introduced to the tip of capillary 6, it is located in the region of micro-plasma beam 2. Under the action of micro-plasma beam 2, the caffeine solution containing sodium ions is desorbed from the tip of capillary 6 and ionized. The resulting sample ions enter the mass spectrometer detector through mass spectrometer inlet 1 for detection and analysis. The analysis results are as follows: Figure 8 As shown.

[0067] Depend on Figure 8 As can be seen in the spectrum, in addition to the protonated ion peak [M+H] associated with the compound caffeine, there is also an abnormality. + =195, there is no interference from other signal peaks.

[0068] Comparative Example

[0069] The caffeine solution containing a high concentration of sodium ions in Example 6 was analyzed by mass spectrometry using nano-ESI coupled with a triple quadrupole mass spectrometer (mass analyzer). The analytical results are as follows: Figure 9 As shown.

[0070] Depend on Figure 9 As shown in the figure, mass spectrometry analysis of caffeine solutions containing high concentrations of sodium ions using nano-electrospray ionization (SES) revealed a significant sodium ion addition signal peak [M+Na]. + =217, which shows that the method of the present invention can effectively reduce the severe phenomenon of alkali metal ions caused by the presence of salt, and shows that the present invention has important value and significant progress in identifying and accurately quantifying the spectral signal peaks of target compounds with high concentrations of added salt ions.

[0071] Finally, it should be pointed out that the above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any changes or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in the present invention should be included within the scope of protection of the present invention.

Claims

1. A method of mass spectrometry ionization based on the principle of Tesla coil, characterized in that, The method comprises the following steps: introducing a liquid sample or a gas sample through a sample introduction device, the outlet end of the sample introduction device being located in front of a port of a mass spectrometer sampling inlet at a distance of 3-5 mm from the port, wherein the sample introduction device for introducing the liquid sample has a desorption function; generating a micro plasma beam with a temperature of 20-40 DEG C and a volume of 0.5-1 cubic millimeter by a Tesla coil, and locating the outlet end of the sample introduction device in or around the micro plasma beam generated by the Tesla coil, and the distance between the central axis of the micro plasma beam generated by the Tesla coil and the outlet end of the sample introduction device being 1-3 mm, and the distance between the central axis of the micro plasma beam generated by the Tesla coil and the port of the mass spectrometer sampling inlet being 3-5 mm, so that the liquid sample is desorbed and introduced into the micro plasma beam region for ionization, or the gas sample is directly introduced into the micro plasma beam region for ionization.

2. The method of ionization for mass spectrometry based on the principle of Tesla coil according to claim 1, characterized in that: When the sample is a liquid sample, the sample introduction device is an electrospray needle, an atomizing sheet or a sample introduction capillary with a metal coating on the inner wall.

3. The method for ionization of mass spectrometry based on Tesla coil principle according to claim 1, characterized in that: When the sample is a gas sample, the sample introduction device is a reagent bottle.

Images