A bactericidal composition containing tetramycin and tricyclazole and its application

Abstract

The present invention relates to a tetramycin- and tricyclazole-containing bactericidal composition, comprising tetramycin and tricyclazole as active ingredients, wherein the mass ratio of tetramycin to tricyclazole is 50:1 to 1:300. The composition of the present invention is used for preventing and treating various plant diseases caused by pathogenic fungi and bacteria, reduces the dosage of pesticides, reduces the adverse effects of pesticides on the ecological environment, has important significance for the comprehensive management of rice disease resistance, and has huge economic and social benefits.

Description

Technical Field

[0001] The invention belongs to the technical field of plant protection, and specifically relates to a bactericidal composition containing tetramycin and tricyclazole and application thereof. Background Art

[0002] Rice is the most important food crop in the world, and more than half of the world's population relies on rice for food. The rice planting area in my country accounts for about 1 / 4 of the cultivated land area, and the annual output accounts for about 2 / 5 of the total grain output. During the rice production process, it is attacked by various diseases in nature. Among them, sheath blight, rice blast and bacterial leaf blight are the three most widely distributed and most destructive rice diseases in the world, which seriously affect the high and stable yield of rice.

[0003] At present, there are many varieties of pesticides used for plant protection, and the conventional pesticides for preventing and controlling rice diseases mainly include tricyclazole, carbendazim, jinggangmycin, hexaconazole, difenoconazole, thiophanate-methyl, azoxystrobin, prochloraz, tebuconazole, rice blast fungicide, etc. In recent years, with the increasing uniformity of planting structure, the occurrence of rice diseases has become more and more serious. Since most of the pesticides have made the pathogens resistant when used in the field or due to reuse and abuse, traditional prevention and control measures and methods have been difficult to control.

[0004] Tetramycin is the trade name of the compound oxadiazine. Its English name is tetramycin, and its CAS number is 11076-50-9. It includes four components: A1, A2, B and C. A1 and A2 are tetraene antibiotics of macrolides, B is a peptide pyrimidine nucleotide antibiotic, and C is a nitrogen-containing heterocyclic aromatic antibiotic. Tetramycin preparations contain a variety of antibiotics, including peptide pyrimidine nucleotide antibiotics, which prevent and control fungal diseases; and nitrogen-containing heterocyclic aromatic derivative antibiotics, which improve crop immunity. Tetramycin has systemic antibacterial activity, preventing pathogens from invading and spreading. It has a wide bactericidal spectrum and has a strong killing effect on 26 known pathogenic fungi in three major categories, including flagellates, ascomycetes and Ascomycetes.

[0005] Tricyclazole is a highly systemic protective triazole fungicide that can be quickly absorbed by plant roots, stems, and leaves, and transported to various parts of the plant, and conducted in the xylem and apoplast of the plant. Chemical name: 5-methyl-1,2,4-triazolo[3,4-b]benzothiazole, CAS registration number: 41814-78-2.

[0006] Long-term, repeated and exclusive application of one type of compound to control plant fungal and bacterial diseases will in many cases lead to rapid selectivity of pathogenic bacteria and fungal strains, and some strains will develop adaptive resistance to specific active compounds, resulting in a significant reduction in the control effect of the active compounds, or even complete loss. Compounding ingredients with different mechanisms of action is an effective way to delay pathogen resistance. After screening for indoor activity and field efficacy, the inventors found that a mixture of tetramycin and tricyclazole has excellent indoor activity and field efficacy against rice diseases. Summary of the invention

[0007] The purpose of the present invention is to provide a fungicide composition containing tetramycin and tricyclazole which has synergistic effect, low use cost and good prevention effect.

[0008] The technical scheme of the present invention is as follows: a fungicide composition containing tetramycin and tricyclazole, wherein the fungicide composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is tetramycin and the active ingredient B is tricyclazole;

[0009] Furthermore, the mass ratio of the active ingredient tetramycin to tricyclazole is 50:1 to 1:300;

[0010] Furthermore, the mass ratio of the active ingredient tetramycin to tricyclazole is 10:1 to 1:200;

[0011] Furthermore, the mass ratio of the active ingredient tetramycin to tricyclazole is 1:1 to 1:100;

[0012] Furthermore, the mass ratio of the active ingredient tetramycin to tricyclazole is 1:1 to 1:50;

[0013] Furthermore, the sum of the mass of the active ingredients tetramycin and tricyclazole accounts for 1% to 80% of the sterilization composition;

[0014] Furthermore, the sum of the masses of the active ingredients tetramycin and tricyclazole accounts for 5% to 60% of the sterilization composition.

[0015] A fungicide composition containing tetramycin and tricyclazole, which can be prepared into a formulation form permitted for use in agriculture from active ingredients and auxiliary ingredients of pesticide preparations;

[0016] Further, the auxiliary agent is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist and a carrier;

[0017] The wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, sodium dioctyl sulfosuccinate, alpha olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether, silkworm feces, saponin powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0018] The dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acid, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0019] The emulsifier is selected from one or more of calcium dodecylbenzene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, tristyrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol polyoxyethylene ether phosphate; and / or

[0020] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0021] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0022] The antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0023] The defoamer is selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of fatty alcohols; and / or

[0024] The solvent is selected from one or more of benzene, toluene, xylene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, acetophenone, N-methylpyrrolidone, alkylene carbonate, propylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and deionized water; and / or

[0025] The preservative is selected from one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0026] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0027] The synergist is selected from synergist phosphite, synergist ether; and / or

[0028] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives; and / or

[0029] The above substances are all commercially available.

[0030] A fungicide composition containing tetramycin and tricyclazole, which can be prepared into dosage forms permitted in agriculture, including powders, dispersible tablets, granules, soluble powders, soluble granules, soluble tablets, tablets, emulsions, emulsion granules, water-dispersible granules, wettable powders, microcapsule granules, large granules, water-dispersible tablets, microcapsule suspensions, dispersible liquids, emulsifiable concentrates, oil emulsions, water emulsions, microemulsions, dispersible oil suspensions, oil-dispersible powders, suspensions, suspoemulsions, soluble liquids, ultra-low volume liquids, aqueous preparations, suspended seed coatings, seed treatment dry powders, seed treatment dispersible powders, seed treatment suspensions, and seed treatment liquids;

[0031] Furthermore, the dosage form is any one of a soluble solution, a suspension, a microemulsion, and an aqueous solution.

[0032] Furthermore, the composition is prepared into a soluble solution, and the components and contents thereof are preferably: 0.1% to 10% tetramycin, 1% to 80% tricyclazole, 0% to 8% antifreeze, 0% to 2% wetting agent, and 100% polar organic solvent;

[0033] Further, the composition is prepared into a suspension, and the components and contents thereof are preferably: 0.1% to 10% tetramycin, 1% to 80% tricyclazole, 2% to 10% dispersant, 2% to 10% wetting agent, 0.01% to 2% defoamer, 0% to 2% thickener, 0% to 8% antifreeze agent, and deionized water is supplemented to 100%;

[0034] Further, the composition is prepared into a microemulsion, and the components and contents thereof are preferably: 0.1% to 10% tetramycin, 1% to 80% tricyclazole, 2% to 10% dispersant, 1% to 10% emulsifier, 2% to 10% stabilizer, and deionized water is supplemented to 100%;

[0035] Furthermore, the composition is prepared into an aqueous solution, comprising 0.1% to 10% tetramycin, 1% to 80% tricyclazole, 0% to 5% antifreeze, 0% to 5% wetting agent, and deionized water to 100%;

[0036] Application of a fungicide composition containing tetramycin and tricyclazole, wherein the fungicide composition is used for preventing and controlling plants, seeds, soil, areas, materials or spaces infected or invaded by harmful fungi and bacteria;

[0037] Furthermore, the fungicidal composition is used for preventing and treating plant fungal and bacterial diseases of cereals, rice, fruit trees, flowers or Chinese medicinal materials;

[0038] Furthermore, the fungicidal composition is used for preventing and treating fungal and bacterial diseases of rice;

[0039] Furthermore, the fungal diseases include diseases of Oomycetes, Ascomycetes, Basidiomycetes or Deuteromycetes;

[0040] Further, the Deuteromycetes among the pathogenic fungi include Rhizoctonia, Fusarium, Botrytis, Pyricularia, Cercospora, Bipolaris, and Colletptrichum;

[0041] Furthermore, the fungal and bacterial diseases of rice include: rice blast, rice sheath blight, rice false smut, rice bacterial leaf blight, rice bacterial leaf streak, rice bacterial brown streak, and rice bacterial basal rot;

[0042] Furthermore, the rice fungal disease is preferably rice blast;

[0043] Rice blast is also known as rice fever. The pathogen is gray Magnaporthe grisea, which belongs to Magnaporthe grisea of ​​the phylum Ascomycota. Rice blast can occur throughout the entire growth period of rice, harming seedlings, leaves, nodes, panicle necks, stalks and grains, which are called seedling blast, leaf blast, leaf pillow blast, node blast, panicle neck blast, stalk blast and grain blast respectively. The growth of the affected rice is stunted, the plant shrinks, the heading is abnormal, and the disease occurs during the heading stage. The main damage is that the neck of the rice panicle, the panicle axis and the stalks turn brown, and even necrotize or even break. The affected rice panicles die because the stalks die, and the grains cannot get nutrients, which often causes white panicles or shriveled grains. Rice blast is one of the most widely distributed and most serious rice diseases in rice-growing areas around the world. Rice blast can reduce rice yield and quality, causing serious production reduction or even crop failure, and has become an important factor restricting rice production.

[0044] Furthermore, the rice bacterial disease is preferably rice bacterial blight;

[0045] Rice bacterial blight is a sudden and explosive disease that can infect all organs of rice during the entire growth period. The symptoms can manifest in various forms under different circumstances, including leaf edge (blight) type, acute type, wilting type, midrib type, yellowing type, etc. Bacterial blight is caused by infection with the pathogenic variant of Xanthomonas oryzae pv.oryzae (Xoo for short) in Xanthomonas oryzae. It is currently the most important and most common bacterial disease that endangers rice production safety in Asia and my country.

[0046] The advantages of the present invention are:

[0047] 1) The bactericidal composition of the present invention has good synergistic and lasting effects within a certain range;

[0048] 2) Reducing the amount of pesticides used can reduce pesticide residues on crops and reduce environmental pollution;

[0049] 3) The fungicide composition of the present invention can enhance plant photosynthesis, improve crop disease resistance, increase yield and optimize crop quality. DETAILED DESCRIPTION

[0050] In order to make the purpose, technical solutions and advantages of the present invention more concise and clear, the present invention is described with the following specific embodiments, but the present invention is by no means limited to these embodiments. The embodiments described below are only preferred embodiments of the present invention, which can be used to describe the present invention and cannot be understood as limiting the scope of the present invention. It should be pointed out that any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

[0051] Preparation Examples

[0052] The preparation examples described below are merely preferred preparation examples of the present invention, and the present invention is not limited thereto.

[0053] Preparation Example 1:

[0054] 16% Tetramycin·Tricyclazole soluble solution (0.4+15.6)

[0055] Formula: tetramycin 0.4%, tricyclazole 15.6%, ethanol 10%, tristyrylphenol polyoxyethylene ether 10%, dimethyl sulfoxide 20%, and propylene carbonate to make up the balance to 100%.

[0056] Preparation method: The active ingredient and the solvent are mixed and stirred until the original drug is completely dissolved, and then an emulsifier is added and stirred evenly to obtain the soluble solution of the present invention.

[0057] Preparation Example 2:

[0058] 18% Tetramycin·Tricyclazole Soluble Solution (0.5+17.5)

[0059] Formula: Tetramycin 0.5%, tricyclazole 17.5%, ethanol 3%, dimethyl sulfoxide 10%, N-methylpyrrolidone 20%, tristyrylphenol polyoxyethylene ether 15%, N,N-dimethylformamide 20%, and cyclohexanone to make up the balance to 100%.

[0060] Preparation method: Same as Preparation Example 1.

[0061] Preparation Example 3:

[0062] 20.5% Tetramycin·Tricyclazole SC (0.5+20)

[0063] Formula: Tetramycin 0.5%, tricyclazole 20%, polyoxyethylene polyoxypropylene ether 4%, sodium lignin sulfonate 1.5%, polyoxyethylene styrene phenol ether phosphate ester salt 3%, xanthan gum 0.25%, magnesium aluminum silicate 1.5%, sodium benzoate 1%, propylene glycol 5%, silicone oil 0.5%, deionized water to make up the balance to 100%;

[0064] Preparation method: according to the above formula, water, dispersant and wetting agent are mixed uniformly, and defoamer and antifreeze agent are added successively under stirring, and mixed uniformly by high-speed shearing, and then tetramycin and tricyclazole are added, and shearing and mixing are continued, and then the material is put into a horizontal sand mill for grinding, so that 90% of the material particle size is below 5μm, and then a thickener is added, and shearing is uniformly obtained to obtain the suspension agent of the present invention.

[0065] Preparation Example 4:

[0066] 23% Tetramycin·Tricyclazole SC (0.5+22.5)

[0067] Formula: Tetramycin 0.5%, tricyclazole 22.5%, sodium polycarboxylate 1%, sodium lignin sulfonate 1%, sodium lauryl sulfate 2%, fatty alcohol polyoxyethylene ether phosphate 3%, ether diglycol 4%, glycerol 2%, organic silicon 2%, silicone oil 1%, gelatin 1%, xanthan gum 3%, deionized water to make up the balance;

[0068] Preparation method: Same as Preparation Example 4.

[0069] Preparation Example 5:

[0070] 4% Tetramycin·Tricyclazole Microemulsion (0.2+3.8)

[0071] Formula: 0.2% tetramycin, 3.8% tricyclazole, 20% acetophenone, 3% castor oil polyoxyethylene ether, 15% EO-PO block copolymer, 1% sodium sulfate of fatty alcohol polyoxyethylene ether, 5% ethylene glycol, 0.05% silicone oil, and water to 100%;

[0072] Preparation method: The active ingredients, solvents, emulsifiers, etc. in the above formula are mixed to obtain an oil phase, antifreeze and water are mixed to obtain an aqueous phase, the oil phase is added to the aqueous phase under stirring and stirred evenly, and shearing is continued for 10 minutes, and then a silicone oil defoamer is added and stirred evenly to obtain oil phase particles with small droplets of 0.01 to 0.1 μm, thereby obtaining the microemulsion described in the present invention.

[0073] Preparation Example 6:

[0074] 3.6% Tetramycin·Tricyclazole Microemulsion (0.1+3.5)

[0075] Formula: 0.1% tetramycin, 3.5% tricyclazole, 25% cyclohexanone, 3% castor oil polyoxyethylene ether, 15% EO-PO block copolymer, 1% fatty alcohol polyoxyethylene ether phosphate, 5% glycerol, 0.05% silicone oil, water to 100%;

[0076] Preparation method: Same as Preparation Example 5.

[0077] Preparation Example 7:

[0078] 2.2% tetramycin tricyclazole aqueous solution (0.2+2)

[0079] Formula: 0.2% tetramycin, 2% tricyclazole, 15% ethanol, 10% tristyrylphenol polyoxyethylene ether, 20% alkyl glycoside, and water to make up the balance to 100%.

[0080] Preparation method: Stir the active ingredient and the solvent until they are completely dissolved, then add the emulsifier and stir evenly, and then add water and stir evenly to prepare the aqueous solution of the present invention.

[0081] Preparation Example 8:

[0082] 5.2% tetramycin tricyclazole aqueous solution (0.2+5)

[0083] Formula: 0.2% tetramycin, 5% tricyclazole, 15% ethanol, 8% propylene carbonate, 10% tristyrylphenol polyoxyethylene ether, 20% alkyl glycoside, and water to make up the balance to 100%.

[0084] Preparation method: Same as Preparation Example 7.

[0085] Plant disease control test case

[0086] Test basis: This test was conducted in accordance with the Agricultural Industry Standards of the People's Republic of China (NY / T1156.8-2007 and NY / T1156.6-2006): "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides" Part 8 and Part 6.

[0087] Test reagents: 15% tetramycin stock, 95% tricyclazole technical, all of which are provided by the group's R&D center.

[0088] Test targets: Rice blast, rice sheath blight, rice bacterial leaf blight, and rice bacterial leaf streak.

[0089] Test crops: rice (variety: Jinjing 818), 20 seeds were sown in each pot after germination, and 10 rice seedlings with 3 leaves and 1 heart stage with consistent growth were selected for use.

[0090] Preparation of spore suspension: Culture the rice pathogen on a suitable culture medium, or culture the diseased tissue in a moisturizing manner. After spores are produced, wash the spores with sterile water and filter them through three layers of gauze to make a suspension with a concentration of 1*10 5 Prepare a suspension of spores / mL for later use.

[0091] Drug preparation: The two original drugs were dissolved in acetone, diluted with 0.1% Tween 80 aqueous solution, and set into 5 series of mass concentrations.

[0092] Chemical treatment: spray the liquid medicine evenly on the leaves until they are completely wet, and wait for the liquid medicine to dry naturally before use. Each treatment is 1 pot, repeated 4 times, and a treatment containing only solvent and surfactant but no active ingredient is set as a blank control.

[0093] Inoculation and cultivation: Inoculate 24 hours after treatment with the agent, and spray inoculate with spore suspension.

[0094] Data investigation: graded investigation based on the incidence of blank control.

[0095] The following grading method is used:

[0096] Level 0: no lesions;

[0097] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0098] Level 3: The lesion area accounts for 6% to 15% of the entire leaf area;

[0099] Level 5: The lesion area accounts for 16% to 25% of the entire leaf area;

[0100] Level 7: The lesion area accounts for 26% to 50% of the entire leaf area;

[0101] Level 9: The lesion area accounts for more than 50% of the entire leaf area.

[0102] Data statistics and analysis:

[0103] Based on the survey data, the disease index and prevention and control effect of each treatment were calculated.

[0104] The disease index is calculated according to formula (1):

[0105] X=[∑(Ni×i) / (N×9)]×100................................(1)

[0106] Where:

[0107] X—disease index;

[0108] N i —Number of diseased leaves at each level;

[0109] i—relative level value;

[0110] N—Total number of leaves surveyed.

[0111] The control effect is calculated according to formula (2):

[0112] P=[(CK-PT) / CK]×100................................................(2)

[0113] Where:

[0114] P——control effect, in percentage %;

[0115] CK——blank control disease index;

[0116] PT - drug treatment index.

[0117] In order to show the degree of synergistic effect, the expected value was calculated based on the Colby method, and when the measured value was larger than the expected value, it was determined that there was a synergistic effect.

[0118] The expected value is calculated according to formula (3):

[0119] (E)=P1+P2-P1P2 / 100................................(3)

[0120] Where:

[0121] P1—A single-dose control effect %;

[0122] P2-B single-dose control effect%.

[0123] Test Example 1: Test on the prevention and control of rice blast

[0124] Table 1 Rice blast

[0125]

[0126]

[0127] Test Example 2: Test on the prevention and control of rice false smut

[0128] Table 2 Rice false smut

[0129]

[0130]

[0131] Test Example 3: Test on Controlling Rice Bacterial Blight

[0132] Table 3 Rice bacterial blight

[0133]

[0134]

[0135] Test Example 4: Test on Controlling Rice Bacterial Leaf Streak Disease

[0136] Table 4 Rice bacterial leaf streak

[0137]

[0138]

[0139] Field efficacy

[0140] Rice field efficacy

[0141] Varieties tested in the experiment: Rice (Yanfeng 47);

[0142] Target of control: Rice blast;

[0143] The test site is Beijingzi Town, Donggang City, Liaoning Province. The area has a long history of rice cultivation. Rice blast, false smut and sheath blight are common diseases of local rice cultivation. Rice seedlings were raised in April in the area, and field management followed local practices.

[0144] Application time and weather: July 20, 2020, cloudy, with a maximum temperature of 29°C and a minimum temperature of 20°C; July 27, 2020, with a maximum temperature of 25°C and a minimum temperature of 21°C.

[0145] Application method: Use conventional spraying method to apply the pesticide, and apply the pesticide again 7 days after applying the pesticide, for a total of 2 times. Investigate the incidence base of rice leaf blast before applying the pesticide, and investigate the panicle neck blast at the yellow maturity stage of rice.

[0146] Experimental method: Randomized block arrangement, plot area 36m 2 , repeated 4 times. Five fixed sampling points were taken on the diagonal of each plot, and 5 plants were checked at each point to investigate the total number of ears, diseased ear rate, disease index, and calculate the control effect.

[0147] Grading method of ear neck blast: 9-level grading method

[0148] Level 0: no disease;

[0149] Level 1: More than 5% loss per ear (individual branches and stalks are diseased);

[0150] Level 3: 6% to 20% loss per ear (about 1 / 3 of the branches and stalks are diseased);

[0151] Level 5: 21% to 50% loss per ear (the neck or main axis of the ear is diseased, and the grains are half-shrunken);

[0152] Grade 7: ear loss 51% to 70% (ear neck disease, most of the grains are half-shrunken);

[0153] Level 9: Ear loss of 71% to 100% (white ears due to disease at the ear neck).

[0154] The efficacy is calculated as follows:

[0155] (1) Disease index = ∑[(number of diseased ears at each level × relative level value) / (total number of ears surveyed × 9)] × 100

[0156] (2) Control effect (%) = [1-(pre-drug disease index of blank control area × post-drug disease index of treated area) / (post-drug disease index of blank control area × pre-drug disease index of treated area)] × 100

[0157] Safety survey:

[0158] During the test, observe whether the crops have symptoms of drug damage. If so, record the extent of the damage and accurately describe the symptoms of drug damage to the crops (dwarfing, chlorosis, deformity, etc.). In addition, record the beneficial effects on the crops.

[0159] Table 5 Field efficacy of tetramycin and tricyclazole mixed to control rice diseases

[0160]

[0161] Field efficacy shows (see Table 5): Under the same environmental conditions, the overall field efficacy of different treatments for controlling rice diseases ranged from 56.77% to 75.16% 10 days after the first application of the pesticide, and from 62.63% to 76.25% 10 days after the second application of the pesticide.

[0162] Safety and production increase test

[0163] Each plot has an area of ​​36m 2 At harvest, 4 points were randomly selected from each treatment, with a length of 1 m 2 Measure actual production, convert it into output, and calculate the rate of increase.

[0164] Analysis of the effect of different treatments on increasing rice yield

[0165] Table 6 Comparison of rice yields under different treatments

[0166] deal with Pharmacy Plot yield / kg <![CDATA[Converted yield / kg·hm 2 > Compared with CK ± / % A 16% Tetramycin·Tricyclazole soluble solution (0.4+15.6) 23.06 6405.59A 11.83 B 18% Tetramycin·Tricyclazole Soluble Solution (0.5+17.5) 22.85 6347.25AB 10.81 C 20.5% Tetramycin·Tricyclazole SC (0.5+20) 22.94 6372.25A 11.25 D 23% Tetramycin·Tricyclazole SC (0.5+22.5) 22.58 6272.25AB 9.51 E 4% Tetramycin·Tricyclazole Microemulsion (0.2+3.8) 22.51 6252.81AB 9.17 F 3.6% Tetramycin·Tricyclazole Microemulsion (0.1+3.5) 21.99 6108.36C 6.64 G 2.2% tetramycin tricyclazole aqueous solution (0.2+2) 22.05 6125.03C 6.94 H 5.2% tetramycin tricyclazole aqueous solution (0.2+5) 23.11 6419.48A 12.08 I 0.3% tetramycin aqueous solution 21.22 5894.47D 2.91 J 75% tricyclazole water dispersible granules 21.03 5841.70D 1.99 CK Clear water control 20.62 5727.81E /

[0167] The test results show that the use of the above-mentioned test agents once at the rice head-breaking stage and the heading stage respectively is safe for rice, has a promoting effect on rice growth, has good control effects on rice blast and sheath blight, and has a significant yield-increasing effect compared with the control agent. It can be widely promoted and applied in rice production.

[0168] The results of the above indoor activity tests and field tests show that the tetramycin and tricyclazole compounded mixture of the present invention exhibits a good control effect on fungal diseases of crops, especially fungal diseases of semi-compartmental fungi. The fungicidal mixture obtained by compounding the present invention has a significant control effect, and has the characteristics of high efficiency, broad spectrum, long lasting effect, strong systemicity, etc. In addition, no compound agent was found to cause phytotoxicity to crops in the test, indicating that when the fungicidal synergistic effect of the obtained fungicidal mixture or preparation is improved, the production cost and use cost can be reduced, and it is safe for crops.

[0169] Although the present invention has been described in detail above with general instructions and specific implementation schemes, it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A bactericidal composition containing tetramycin and tricyclazole, characterized in that: The bactericidal composition consists of an active ingredient A and an active ingredient B, wherein the active ingredient A is tetramycin and the active ingredient B is tricyclazole; the mass ratio of the tetramycin to the tricyclazole is 1:1-1:

50.

2. The bactericidal composition according to claim 1, characterized in that The sum of the mass of the active ingredients tetramycin and tricyclazole accounts for 1% to 80% of the sterilization composition.

3. The bactericidal composition according to claim 1, characterized in that The sum of the mass of the active ingredients tetramycin and tricyclazole accounts for 5% to 60% of the sterilization composition.

4. The bactericidal composition according to any one of claims 1 to 3, characterized in that The bactericidal composition contains not only effective ingredients but also pesticide auxiliary ingredients.

5. The bactericidal composition according to claim 4, characterized in that The bactericidal composition can be prepared into a dosage form permitted in agriculture, and the dosage form is any one of a soluble solution, a suspension, a microemulsion, and an aqueous solution.

6. Use of the bactericidal composition according to claim 1, characterized in that: The bactericidal composition is used for preventing and treating fungal and bacterial diseases of rice, and the fungal and bacterial diseases of rice include rice blast, rice sheath blight, rice bacterial leaf blight and rice bacterial leaf streak.

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