A pesticide composition containing trichlorfon, bronopol and cartap and its application

Abstract

The present invention discloses a pesticide composition containing trichlorfonazole, bronopol and cartap and its application, comprising effective ingredients trichlorfonazole, bronopol and cartap, wherein the mass ratio of trichlorfonazole and bronopol with fungicidal activity to cartap with insecticidal activity is 1-10:1-10:1-10. The present invention also discloses the application of the pesticide composition in preventing and treating rice seedling rot or / and rice seedling rot or / and rice dry tip nematode disease or / and rice bacterial base rot. The active ingredients of the pesticide composition of the present invention have different action mechanisms, expand the prevention and control spectrum, and have significant synergistic synergy. The resistance of rice seedling pathogens to fungicides such as carbendazim, prochloraz and cyanothiocarb can be effectively controlled, and the resistance control of rice seedling rot in my country has great application value. It is effective and has significant efficiency in preventing and controlling rice seedling rot, rice seedling blight, rice stem tip nematode disease and rice bacterial base rot. It can reduce the use of pesticides, has high quality and low price, and is environmentally friendly and safe.

Description

Technical Field

[0001] The invention relates to the field of pesticide compositions, in particular to a pesticide composition containing trichlorfon, bronopol and cartap. Background Art

[0002] Rice seedling rot occurs to varying degrees in all rice-producing areas in my country, and is particularly serious in northern rice-producing areas. Due to abnormal climate and improper management, many rice-producing areas often suffer from severe seedling rot, resulting in the destruction of seeds and re-sowing, seed waste, hindering variety layout, delaying farming time, and seriously affecting rice yield and quality. Rice seedling rot is a general term for the death of seeds, young sprouts and seedlings in the nursery field (i.e. rotten seeds, rotten sprouts and dead seedlings), which can be divided into two categories: physiological and infectious. Physiological seedling rot refers to diseases caused purely by adverse environmental conditions; infectious seedling rot is mainly caused by Achlya spp., Pythium spp. and Fusarium spp. In rice production, large-scale rotten sprouts and dead seedlings are mostly infectious diseases.

[0003] Rice bakanae disease, caused by the Gibberella fujikurois species complex, is a serious seed-borne disease of rice. The disease occurs in rice-growing areas around the world and can cause a 3.0% to 95.4% reduction in rice production, or even a complete loss of yield. In addition, the Gibberella fujikurois species complex strains also threaten human and animal health by producing toxins such as fumonisins. Due to the lack of effective disease-resistant varieties, the prevention and control of rice bakanae disease is still mainly based on seed treatment with chemical agents. In my country, benzimidazole fungicides such as carbendazim have been widely used in the prevention and control of rice bakanae disease since the 1970s, but their efficacy has been greatly reduced due to the resistance of the pathogen. In the 1980s, imidazole fungicides, especially prochloraz, were widely used in production because of their excellent efficacy against rice seedling disease and no cross-resistance with carbendazim. However, as the efficacy of the drugs decreased, the frequency of resistance of pathogenic bacteria strains in the field (MIC>3.125μg / mL) was higher than 80%. Cyanoacrylate fungicides, cyproconazole, were synthesized by the Jiangsu Pesticide Research Institute in 1998. They have extremely high selectivity and Fusarium specificity, no cross-resistance with carbendazim and prochloraz, and excellent efficacy against rice seedling disease. Pathogen resistance caused by myosin-5 point mutations has caused the frequency of resistance to cyproconazole in rice seedling pathogens in Zhejiang Province (Jinhua City, Shaoxing City and Jiaxing City) to increase from 18% in 2017 to 47% in 2018; cyproconazole has been used for less than 10 years, but rice seedling pathogens in many regions have developed obvious resistance and the resistant population is still expanding rapidly. At present, there are more than 190 pesticides (including single agents and compound agents) registered by the Pesticide Testing Institute of the Ministry of Agriculture and Rural Affairs for the prevention and control of rice seedling disease. However, about 85% of the active ingredients in the preparations are prochloraz, fludioxonil, carbendazim or thiram, and the types of active ingredients in the preparations are relatively small. The situation of effective prevention and control of rice seedling disease and resistance management is still very severe.

[0004] White tip nematode disease, caused by Aphelenchoides besseyi Christie, is an important seed-transmitted ectoparasitic nematode on rice. The typical symptoms of white tip disease are: the leaves on the upper part of the diseased plant, especially the tip of the sword leaf, turn pale yellow to pale, and then twist into an off-white tip, with a curved brown boundary line at the junction of the diseased and healthy leaves. The disease can cause the rice plant to become shorter, the sword leaf to shorten or twist into an off-white tip, the strong rice ears to become shorter, the number of grains to decrease, the number of barren grains to increase, and the "spikelet" phenomenon to appear, affecting the yield and quality of rice.

[0005] Rice bacterial foot rot, caused by Dickeyazeae (synonym Erwinia chrysanthemipv.zeae), is one of the most important diseases of rice. It has been reported in some Southeast Asian countries and 15 provinces (municipalities) in China, and some areas have suffered serious damage. Related studies have shown that rice bacterial foot rot can survive in soil, field water and diseased residues, but the main overwintering place is diseased rice straw and soil containing diseased residues. At the same time, infected seeds are also one of the main initial infection sources of rice bacterial foot rot. The typical symptoms of this disease are that the base of the plant stem and the root node turn brown or dark brown, accompanied by a foul odor. The disease can occur from the tillering stage to the ear stage. The diseased rice has fewer tillers, the base of the stem rots, and produces white ears and barren grains. Severe cases can cause large-scale rice harvest failure, which has affected rice production to varying degrees. Cultivating disease-free and strong seedlings is one of the key measures to prevent and control bacterial rot of rice. Therefore, proper seed treatment before rice sowing can economically and efficiently prevent the occurrence and harm of diseases.

[0006] At present, there are many pesticides that can be used to control the above-mentioned rice seedling rot, seedling blight, dry tip nematode disease, and rice bacterial basal rot separately, but there is no research progress on pesticides that can effectively control all the above diseases at the same time. Summary of the invention

[0007] Purpose of the invention: In order to solve the problems existing in the prior art, the present invention proposes a pesticide composition containing thiophanate-methyl, bronopol and cartap, the active ingredients have different action mechanisms, the control spectrum is expanded, the synergistic effect is significant, and the resistance of rice seedling rot fungi to fungicides such as carbendazim, prochloraz and cyanocobalamin can be effectively controlled, and the resistance control of rice seedling rot in my country has great application value, and the effect and synergy are obvious in preventing and controlling rice seedling rot, rice seedling rot, rice dry tip nematode disease and rice bacterial base rot can be significantly reduced, the amount of pesticides used can be reduced, the quality is high, the price is low, it is environmentally friendly and safe, and the economic, ecological and social benefits are significant.

[0008] Another object of the present invention is to provide the use of the pesticide composition in preventing and controlling rice seedling rot and / or rice seedling blight and / or rice stem tip nematode disease and / or rice bacterial basal rot.

[0009] Technical solution: To achieve the above purpose, the present invention adopts the following technical solution: a pesticide composition containing trichlorfonazole, bronopol and cartap, comprising the following effective ingredients: trichlorfonazole, bronopol and cartap; the mass ratio of trichlorfonazole, bronopol and cartap is 1-10:1-10:1-10.

[0010] Triticonazole is a triazole fungicide developed and manufactured by Bayer. It is an inhibitor of ergosterol biosynthesis and has the characteristics of high efficiency, broad spectrum, strong systemicity, and dual protective and therapeutic effects. At present, it is mainly used to control diseases of cereal crops, leguminous crops, and fruit trees, and is particularly effective against seed-borne diseases. However, there is no registered product in my country that uses triticonazole to control rice diseases such as rice seedling blight.

[0011] Bronopol, scientifically known as bronopol, is a broad-spectrum bactericide of the bromononitroalcohol class. It can inhibit the activity of dehydrogenase by oxidizing the sulfhydryl groups in pathogenic enzymes, thereby inhibiting the activity of pathogens. It has the characteristics of low dosage, high activity, good compatibility, good water solubility and a wide pH range of use. It is one of the cosmetic preservatives approved for use by the U.S. Food and Drug Administration (FDA). It has no irritation or allergic reaction to human skin and is also widely used in agricultural production.

[0012] Cartap, also known as Badan, is a nereis toxin insecticide with strong stomach poisoning effect. It also has contact killing and certain antifeedant and egg-killing effects. It knocks down pests quickly, has a long residual effect, and has a broad spectrum of insecticides. No carcinogenic, teratogenic, or mutagenic effects have been observed.

[0013] Preferably, the mass ratio of trichlorfonazole, bronopol and cartap is 1-2:1-4:1-6 or 1:1:10; further preferably, the mass ratio of trichlorfonazole, bronopol and cartap is 1:1-4:4.

[0014] Preferably, the pesticide composition further contains an adjuvant.

[0015] The percentage content of the active ingredient in the pesticide composition calculated by mass is 10% to 48%, preferably 20 to 32%.

[0016] Preferably, the pesticide composition is in the form of a wettable powder or a dispersible powder for seed treatment.

[0017] In some embodiments, for wettable powders, the adjuvant is selected from one or more of fillers, wetting agents, dispersants, penetrants, stabilizers, and color warning agents; for dispersible powders for seed treatment, the adjuvant is selected from one or more of fillers, wetting agents, dispersants, penetrants, film-forming agents, stabilizers, and color warning agents.

[0018] Preferably, in some embodiments, for wettable powders, the filler is selected from bentonite, the wetting agent is selected from sodium dodecylbenzene sulfonate, the dispersant is selected from calcium lignin sulfonate, the penetrant is selected from tridecanol polyoxyethylene ether, the stabilizer is selected from tributyl phosphate, and the color warning agent is selected from Hessul Green G1.

[0019] Preferably, in some embodiments, for the dispersible powder for seed treatment, the filler is selected from kaolin, the wetting agent is selected from pull open powder BX, the dispersant is selected from sodium styrene sulfonate, the penetrant is selected from sodium diethyl succinate sulfonate, the film-forming agent is selected from polyvinyl alcohol, the stabilizer is selected from isopropyl phosphate, and the color warning agent is selected from carmine.

[0020] In some embodiments, the wettable powder comprises, by mass percentage, 1% to 30% of trichlorfonazole, 1% to 22.5% of bronopol, 1.5% to 17% of cartap, 5% to 6% of wetting agent, 5% to 6% of dispersant, 0.2% to 0.5% of penetrant, 0.5% to 1% of stabilizer, 0.2% to 1% of color alarm agent, and 100% of filler; preferably, the wettable powder comprises 4% to 5% of trichlorfonazole, 8% to 10% of bronopol, 10% to 12% of cartap, and 10% to 15% of wetting agent. The wettable powder comprises: 1% to 2% of trichlorfon, 1% to 2% of bronopol, 15% to 17% of cartap, 5% to 6% of wetting agent, 5% to 6% of dispersant, 0.2% to 0.3% of penetrant, 0.5% to 0.7% of stabilizer, 0.2% to 0.5% of warning agent, and 100% of filler; or, the wettable powder comprises: 1% to 2% of trichlorfon, 1% to 2% of bronopol, 15% to 17% of cartap, 5% to 6% of wetting agent, 5% to 6% of dispersant, 0.2% to 0.3% of penetrant, 0.5% to 0.7% of stabilizer, 0.2% to 0.6% of warning agent, and 100% of filler;

[0021] In some embodiments, the seed treatment dispersible powder comprises, by mass percentage: 2% to 6% of trichlorfon, 2% to 6% of bronopol, 3% to 10% of cartap, 5% to 7% of wetting agent, 5% to 7% of dispersant, 0.2% to 0.5% of penetrant, 1% to 2% of film-forming agent, 0.5% to 1% of stabilizer, 0.2% to 1% of color warning agent, and the filler makes up 100%; preferably, the seed treatment dispersible powder comprises: 2.5% to 3% of trichlorfon, 2.5% to 3% of bronopol, 5% to 9% of cartap, 5% to 6% of wetting agent, 5% to 6% of dispersant, 0.3% to 0.5% of penetrant, 1.5% to 2% of film-forming agent, 0.8% to 1% of stabilizer, 0.4% to 0.8% of color warning agent, and the filler makes up 100%.

[0022] The present invention also provides the use of the pesticide composition in preventing and controlling rice seedling rot and / or rice seedling rot and / or rice stem tip nematode disease.

[0023] The invention also provides application of the pesticide composition in preventing and controlling rice bacterial basal rot.

[0024] The application comprises: diluting the pesticide composition and then soaking seeds.

[0025] Preferably, the rice seed treatment agent is diluted 400 to 800 times, 500 to 1000 times or 1000 to 2000 times with water and then soaked. After dilution, the concentration of the active ingredient is 125 to 250 μg / mL, 190 to 380 μg / mL, 220 to 440 μg / mL, or 120 to 240 μg / mL. The soaking time is 24 to 72 hours, and further 36 to 48 hours. The rice variety is Huaidao No. 11 and / or Yangfujing No. 8.

[0026] Beneficial effects:

[0027] Compared with the prior art, the pesticide composition containing trichlorfon, bronopol and cartap of the present invention has the following advantages:

[0028] (1) The product is novel and the product development is feasible: In recent years, with the implementation of my country's new pesticide registration policy, pesticide registration management has become more stringent and scientific. The pesticide composition provided by the present invention is compounded by chloranil, bronopol and cartap, and the active ingredients have different mechanisms of action, are safe and efficient, and currently no chloranil has been registered for the prevention and treatment of rice diseases such as rice seedling blight. The innovative introduction of chloranil in the prevention and treatment of rice diseases and the rational compounding of chloranil, bronopol and cartap are in line with my country's new pesticide registration policy, and the feasible development of new products can provide an economical and efficient agent selection for the prevention and treatment of diseases such as rice seedling rot and / or rice seedling blight and / or rice dry tip nematode disease and / or rice bacterial basal rot in China.

[0029] (2) Expand the control spectrum, reduce pesticides and increase efficacy: chloranil and bronopol have a killing effect on pathogens, but have little effect on nematodes; cartap has a high killing effect on rice stem tip nematodes, but has little effect on rice seedling rot and / or rice seedling rot and / or rice bacterial basal rot. Rice seed treatment agents containing chloranil, bronopol and cartap can simultaneously control rice seedling rot and / or rice seedling rot and / or rice stem tip nematode and / or rice bacterial basal rot, and both the fungicide and insecticide effects are significantly enhanced, which can reduce the amount of pesticides used, is environmentally friendly and safe, and has significant economic, ecological and social benefits.

[0030] (3) Delaying the generation and development of drug resistance, which is of great significance for the management of rice seedling disease resistance: The pesticide composition provided by the present invention is prepared by compounding chloranil, bronopol and cartap, which can effectively manage the resistance of rice seedling disease fungi to fungicides such as carbendazim, prochloraz and cyanothiocarb, and can be used in rotation with a variety of existing agents; at the same time, it reduces the potential resistance of rice seedling disease fungi such as chloranil and bronopol, respectively, and prolongs the service life of the agents, which is of great significance for the management of rice seedling disease resistance in my country.

[0031] (4) High quality and low price, with great value for promotion and application: Methionazole has the characteristics of high efficiency, broad spectrum, strong systemicity, dual effects of protection and treatment, and is particularly effective against seed-borne diseases. It still occupies an important position among triazole fungicides and is inexpensive. Bronopol is a bromonitroalcohol fungicide with high activity, good compatibility, good water solubility, a wide pH range of use, and good safety. It is also widely used in agricultural production and is inexpensive. Cartap still occupies a dominant position in the prevention and control of rice stem tip nematode disease. Reasonable compounding of the three and feasible development of new products can effectively solve the problems existing in the prevention and control of rice seed-borne diseases and rice bacterial basal rot, with low cost and great value for promotion and application. DETAILED DESCRIPTION

[0032] The present invention is further explained below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and are not used to limit the scope of the present invention. After reading the present invention, various equivalent forms of modifications to the present invention by those skilled in the art all fall within the scope defined by the claims attached to this application.

[0033] Bioassay Example 1: Indoor activity assay test.

[0034] Antifungal activity of single agents and ternary combinations of trichlorfonazole, bronopol and cartap against rice bakanae pathogen:

[0035] (1) Test strain: Fusarium fujikuroi, collected from rice fields in Jurong City, Jiangsu Province, isolated, identified, and stored by the Plant Protection Research Laboratory of Zhenjiang Agricultural Science Research Institute in the hilly area of ​​Jiangsu Province. Other strains of Fusarium fujikuroi can also be used. The strain was stored on potato sucrose agar (PSA) slant (4°C).

[0036] (2) Test agents: 96% (W / W) chlorpyrifos technical, Jiangsu Agricultural Hormone Engineering Technology Research Center Co., Ltd.; 99% (W / W) bronopol technical, Shandong Yousuo Chemical Technology Co., Ltd.; 98% (W / W) cartap technical, Lianyungang Liben Crop Technology Co., Ltd.

[0037] (3) Test method: With reference to the Guidelines for Pesticide Bioassays NY / T1156.2-2006, the mycelium growth rate method was used to test the toxicity of the single agent and the three-component combination agents of different ratios against the rice seedling pathogen.

[0038] (4) Data analysis: The DPS13.0 professional version data processing system was used to calculate the regression equation, EC and the inhibitory effect of single agent and three-component compound agents of different ratios on the mycelial growth of rice seedling pathogen. 50 and its 95% confidence limits.

[0039] According to the Wadley method, the synergistic coefficient (SR) was calculated. The synergistic coefficient (SR) was used to evaluate the type of combined action of mixed drugs, that is, SR < 0.5 was antagonistic, 0.5 ≤ SR ≤ 1.5 was additive, and SR > 1.5 was synergistic. SR = EC 50 (Eth) / EC 50 (Eob), EC 50 (Eth) = (a + b + c) / [(a / E 50 A)+(b / EC 50 B)+(c / EC 50 C)] where A, B, and C are the individual doses of the drug, a, b, and c are the proportions of the corresponding individual doses in the mixture, and EC 50 (Eth) is the mixed agent EC 50 Theoretical value, EC 50 (Eob) is the mixed EC 50 Measured value.

[0040] (5) Results and analysis:

[0041] Table 1 Test results of the biological activity (toxicity) of trichlorfonazole, bronopol, cartap and their mixtures against rice seedling pathogen

[0042]

[0043]

[0044] In Table 1, the mass ratio of chlorpyrifos:bromo:kill is the mass ratio of chlorpyrifos, bronopol and cartap.

[0045] The results of laboratory tests showed that the EC values ​​of trichlorfon, bronopol and cartap for inhibiting the mycelial growth of rice bakanae pathogen were 50 The values ​​were 0.1404, 13.3644 and 1049.1307 μg / mL, respectively. The mass ratio of trichlorfonazole, bronopol and cartap was between 1-10:1-10:1-10, and the synergistic coefficient of the compound against rice seedling pathogen was between 0.8230 and 2.1419, which had an additive or synergistic effect; when the mass ratio was between 1-4:1-4:1-4, the synergistic coefficient of the compound against rice seedling pathogen was greater than 1.5, which had a synergistic effect (Table 1). Based on the characteristics of trichlorfonazole, bronopol and cartap and the results of indoor ratio screening, it was preliminarily determined that the optimal mass ratio of trichlorfonazole, bronopol and cartap was set between 1-4:1-4:1-4.

[0046] Preparation Example 1: Preparation of 24% trichlorfonazole·bronopol·carbap WP.

[0047] Calculated by mass percentage, the distribution ratio of each component of the wettable powder is: 2% chlorpyrifos, 2% bronopol, 20% cartap, 6% sodium dodecylbenzene sulfonate (wetting agent), 5% calcium lignin sulfonate (dispersant), 0.5% tridecyl alcohol polyoxyethylene ether (penetrant), 0.5% tributyl phosphate (stabilizer), 0.2% hexachlorobenzene G1 (warning color agent), and bentonite (filler) makes up 100%.

[0048] Preparation method:

[0049] The materials were prepared according to the above ratio and processed by a conventional wettable powder preparation method to prepare 12% trichlorfonazole·bronopol·carbazone WP.

[0050] Preparation Example 2: Preparation of 36% trichlorfonazole·bronopol·carbap WP.

[0051] Calculated by mass percentage, the distribution ratio of each component of the wettable powder is: 30% chlorpyrifos, 3% bronopol, 3% cartap, 5% sodium dodecylbenzene sulfonate (wetting agent), 6% calcium lignin sulfonate (dispersant), 0.8% tridecyl alcohol polyoxyethylene ether (penetrant), 0.6% tributyl phosphate (stabilizer), 0.3% hexachlorobenzene G1 (warning agent), and bentonite (filler) makes up 100%.

[0052] Preparation method:

[0053] The materials were prepared according to the above ratio and processed by a conventional wettable powder preparation method to prepare 36% trichlorfonazole·bronopol·carbazone WP.

[0054] Preparation Example 3: Preparation of 48% trichlorfonazole·bronopol·carbap WP.

[0055] Calculated by mass percentage, the distribution ratio of each component of the wettable powder is: 4% chlorpyrifos, 40% bronopol, 4% cartap, 8% sodium dodecylbenzene sulfonate (wetting agent), 4% calcium lignin sulfonate (dispersant), 0.9% tridecyl alcohol polyoxyethylene ether (penetrant), 0.7% tributyl phosphate (stabilizer), 0.4% hexachloride G1 (warning color agent), and bentonite (filler) makes up 100%.

[0056] Preparation method:

[0057] The materials were prepared according to the above ratio and processed by a conventional wettable powder preparation method to prepare 48% trichlorfonazole·bronopol·carbazone WP.

[0058] Preparation Example 4: Preparation of 22% trichlorfonazole·bronopol·carbap WP.

[0059] Calculated by mass percentage, the distribution ratio of each component of the wettable powder is: 3% chlorpyrifos, 9% bronopol, 10% cartap, 7% sodium dodecylbenzene sulfonate (wetting agent), 5% calcium lignin sulfonate (dispersant), 1% tridecyl alcohol polyoxyethylene ether (penetrant), 0.8% tributyl phosphate (stabilizer), 0.5% hexachlorobenzene G1 (warning agent), and bentonite (filler) makes up 100%.

[0060] Preparation method:

[0061] The materials were prepared according to the above ratio and processed by a conventional wettable powder preparation method to prepare 22% trichlorfonazole·bronopol·carbazone WP.

[0062] Preparation Example 5: Preparation of 10% trichlorfonazole·bronopol·carbapide ZF.

[0063] Calculated by mass percentage, the distribution ratio of each group of the dispersible powder for seed treatment is: 2% thiazolinone, 3% bronopol, 5% cartap, 7% sodium styrene sulfonate (dispersant), 5% lakai powder BX (wetting agent), 0.4% sodium diethyl succinate sulfonate (penetrant), 1.5% polyvinyl alcohol (film-forming agent), 0.5% isopropyl phosphate (stabilizer), 0.4% carmine (warning color agent), and kaolin (filler) makes up 100%.

[0064] Preparation method:

[0065] The materials were prepared according to the above ratio and processed by a conventional seed treatment dispersible powder preparation method to prepare 10% trichlorfonazole·bronopol·carbapide ZF.

[0066] Preparation Example 6: Preparation of 19% trichlorfonazole·bronopol·carbapide ZF.

[0067] Calculated by mass percentage, the distribution ratio of each group of the dispersible powder for seed treatment is: 3% thiazolinone, 6% bronopol, 10% cartap, 8% sodium styrene sulfonate (dispersant), 6% lakai powder BX (wetting agent), 0.6% sodium diethyl succinate sulfonate (penetrant), 1.2% polyvinyl alcohol (film-forming agent), 0.8% isopropyl phosphate (stabilizer), 0.5% carmine (warning color agent), and kaolin (filler) makes up 100%.

[0068] Preparation method:

[0069] The materials were prepared according to the above ratio and processed by a conventional seed treatment dispersible powder preparation method to prepare 19% trichlorfonazole·bronopol·carbapide ZF.

[0070] Preparation Example 7: Preparation of 24% trichlorfonazole·bronopol·carbapide ZF.

[0071] Calculated by mass percentage, the distribution ratio of each group of the dispersible powder for seed treatment is: 8% thiazolinone, 8% bronopol, 8% cartap, 9% sodium styrene sulfonate (dispersant), 7% lakai powder BX (wetting agent), 0.8% sodium diethyl succinate sulfonate (penetrant), 1.5% polyvinyl alcohol (film-forming agent), 0.5% isopropyl phosphate (stabilizer), 0.3% carmine (warning color agent), and kaolin (filler) makes up 100%.

[0072] Preparation method:

[0073] The materials were prepared according to the above ratio and processed by a conventional seed treatment dispersible powder preparation method to prepare 24% trichlorfonazole·bronopol·carbapide ZF.

[0074] Field efficacy example 1: Field efficacy test of a pesticide composition containing trichlorfon, bronopol and cartap against rice seedling rot, seedling blight and dry tip nematode disease.

[0075] The pesticide compositions containing trichlorfonazole, bronopol and cartap in Preparation Examples 1-7 were respectively compared with 20% trichlorfonazole·carb WP (trichlorfonazole and cartap original drugs were prepared in a 1:1 ratio according to the method for preparing a wettable powder in Example 4) and 20% bronopol·carb WP (bronopol and cartap original drugs were prepared in a 1:1 ratio according to the method for preparing a wettable powder in Example 5) as binary compound agents, and 28% trichlorfonazole FS (Shanghai Yuelian Biotechnology Co., Ltd.), 20% bronopol WP (Dandong Mingzhu Technology Co., Ltd.) and 6% cartap AS (Jiangsu Green Shield Plant Protection Pesticide Experiment Co., Ltd.) were used as control agents to carry out field efficacy tests for preventing and controlling rice seedling rot, seedling rot and dry tip nematode disease.

[0076] Experimental treatment method: The experimental site is located in the experimental field in the Agricultural Science and Technology Innovation Center Park of Zhenjiang Agricultural Science Research Institute in the hilly area of ​​Jiangsu Province, Baitu Town, Jurong City, Jiangsu Province. The soil is horse liver soil with medium fertility and an organic matter content of about 1.85%. The rice variety is Huaidao No. 11. The experiment is set up with 13 treatments, namely: Preparation Examples 1-7, the dosage of the drug treatment is set to be 125 μg / mL (based on the mass concentration of the main bactericidal components of chloranil and bronopol as 125 μg / mL), 20% chloranil·carbamido WP, 20% bronopol·carbamido WP, 28% chloranil FS, 20% bronopol WP and 6% carbamido AS treatment dosages are based on chloranil, bronopol, chloranil, bronopol and carbamido mass concentrations of 125 μg / mL, respectively, and clear water is used as a blank control. 500g of naturally diseased 'Huaidao No. 11' rice seeds were soaked in 1L of the above 13 treatment solutions or water for 48h, and then germinated for about 48h until the buds were half a grain long and sown in a 5m×1m seedbed. Each treatment was repeated 3 times. When the seedlings were 30 days old, the seedlings of each treatment were transplanted to a 20m×2m field plot with a row spacing of about 20cm×15cm, and each treatment was randomly arranged. During the rice growth period, fertilizer, water and other pests and diseases were managed as usual. The rate of rice seedling rot was investigated before transplanting, and the rate of rice seedling rot and dry tip nematode were investigated during the heading and filling period. The control effect was calculated and the DPSv7.05 data processing system was used to perform variance analysis of the control effect using the Duncan new multiple range method. The calculation formulas for diseased plant rate and control effect are: diseased plant rate = number of diseased plants / total number of plants surveyed × 100%; control effect = [(control diseased plant rate - treatment diseased plant rate) / control diseased plant rate] × 100%.

[0077] The test results show that the field control effects of the 12 agents on rice seedling rot and seedling bakan are as follows from high to low: Preparation Example 7, Preparation Example 5, Preparation Example 6, Preparation Example 4, Preparation Example 1, Preparation Example 2, Preparation Example 3, 28% trichlorfonazole FS, 20% trichlorfonazole·cartap WP, 20% bronopol·cartap WP, 20% bronopol WP and 6% cartap AS. The field control effects of Preparation Examples 1-7 on rice seedling rot and seedling bakan are higher than the control effects of the binary ratio agents 20% trichlorfonazole·cartap WP and 20% bronopol·cartap WP and the control agents 28% trichlorfonazole FS, 20% bronopol WP and 6% cartap AS, and the differences are significant (Table 2). The protective effects of Preparation Example 1, Preparation Example 5 and Preparation Example 6 on rice stem tip nematode disease were higher than those of 20% chlorfenapyr·cartap WP, 20% bronopol·cartap WP and 6% cartap AS, and the differences were significant; because the effective amount of cartap, an insecticidal active ingredient, was significantly lower, the protective effects of Preparation Examples 2, 3, 4 and 7 on rice stem tip nematode disease were lower than those of 20% chlorfenapyr·cartap WP, 20% bronopol·cartap WP and 6% cartap AS, and the differences were significant; 28% chlorfenapyr FS and 20% bronopol WP had basically no protective effect on rice stem tip nematode disease (Table 2).

[0078] This shows that the pesticide composition containing trichlorfonazole, bronopol and cartap has excellent control effect on rice seedling rot, seedling bakan and dry tip nematode disease, can play a dual effect of sterilization and insecticide, and after compounding in an optimal ratio, the sterilization effect and insecticide effect are significantly enhanced compared with the binary compound of trichlorfonazole and cartap or bronopol and cartap and the single agent. It can be seen that the pesticide composition of the present invention reduces the use of drugs and increases the efficiency, controls pests on a wide spectrum, can delay the generation of drug resistance of diseases, and has great significance for the comprehensive prevention and control of diseases such as rice seedling rot, seedling bakan and dry tip nematode disease.

[0079] Table 2 Field efficacy of the agent prepared in the examples of the present invention against rice seedling rot, seedling blight and dry tip nematode disease

[0080]

[0081] Note: The treatment dosages of Preparation Examples 1-7 were calculated based on the sum of the mass concentrations of trichlorfonazole and bronopol as 125 μg / mL; the treatment dosages of 20% trichlorfonazole·cartap WP, 20% bronopol·cartap WP, 28% trichlorfonazole FS, 20% bronopol WP and 6% cartap AS were calculated based on the mass concentrations of trichlorfonazole, bronopol, trichlorfonazole, bronopol and cartap as 125 μg / mL, respectively; data in the same column marked with different lowercase letters indicate significant differences (P<0.05).

[0082] Field efficacy example 2: Field efficacy test of a composition containing a preferred ratio of trichlorfon, bronopol and cartap against rice seedling rot, seedling rot and dry tip nematode disease.

[0083] The preferred pesticide compositions containing trichlorfonazole, bronopol and cartap of Preparation Examples 4-7 were respectively prepared with 20% trichlorfonazole·carb WP (trichlorfonazole and cartap original drugs were prepared in a 1:1 ratio according to the method for preparing a wettable powder in Example 4) and 20% bronopol·carb WP (bronopol and cartap original drugs were prepared in a 1:1 ratio according to the method for preparing a wettable powder in Example 5) as binary compound agents for comparison, and 16% prochloraz·carb WP (prochloraz: cartap = 1:3) (Jiangsu Green Shield Plant Protection Pesticide Experiment Co., Ltd.) and 20% cyproconazole·carb WP (cyproconazole: cartap = 1:1) (Jiangsu Green Shield Plant Protection Pesticide Experiment Co., Ltd.) were used as control agents to carry out field efficacy tests for the prevention and control of rice seedling rot, seedling rot and dry tip nematode disease.

[0084] Experimental treatment method: The experimental site is located in the experimental field in the Agricultural Science and Technology Innovation Center Park of Zhenjiang Agricultural Science Research Institute in the Jiangsu Hilly Area of ​​Baitu Town, Jurong City, Jiangsu Province. The soil is horse liver soil with medium fertility and an organic matter content of about 1.85%. The rice variety is Huaidao No. 11. The experiment has 13 treatments, namely: 500 and 1000 times of Preparation Example 4, 400 and 800 times of Preparation Example 5, 500 and 1000 times of Preparation Example 6, 1000 and 2000 times of Preparation Example 7, 20% trichlorfonazole·carbamido WP 2000 times, 20% bronopol·carbamido WP 200 times, 16% prochloraz·carbamido WP 500 times (recommended dose), 20% cyanothiocarb·carbamido WP 1000 times (recommended dose) and clear water blank control. 500g of naturally diseased 'Huaidao No. 11' rice seeds were soaked in 1L of the above 13 treatment solutions or water for 48h, and then germinated for about 48h until the buds were half a grain long and sown in a 5m×1m seedbed. Each treatment was repeated 3 times. When the seedlings were 30 days old, the seedlings of each treatment were transplanted to a 20m×2m field plot with a row spacing of about 20cm×15cm, and each treatment was randomly arranged. During the rice growth period, fertilizer, water and other pests and diseases were managed as usual. The rate of rice seedling rot was investigated before transplanting, and the rate of rice seedling rot and dry tip nematode were investigated during the heading and filling period. The control effect was calculated and the DPSv7.05 data processing system was used to perform variance analysis of the control effect using the Duncan new multiple range method. The calculation formulas for diseased plant rate and control effect are: diseased plant rate = number of diseased plants / total number of plants surveyed × 100%; control effect = [(control diseased plant rate - treatment diseased plant rate) / control diseased plant rate] × 100%.

[0085] The test results show that the field control effects of the 12 drug treatments on rice seedling rot and seedling blight are as follows from high to low: Preparation Example 4, 500 times liquid; Preparation Example 5, 400 times liquid; Preparation Example 6, 500 times liquid; Preparation Example 7, 1000 times liquid; Preparation Example 4, 1000 times liquid; Preparation Example 5, 800 times liquid; Preparation Example 6, 1000 times liquid; Preparation Example 7, 2000 times liquid; 20% chlorpyrifos·carb WP 2000 times liquid; 20% cyanoacrylate·carb WP 1000 times liquid; 20% bronopol·carb WP 200 times liquid; and 16% prochloraz·carb WP 500 times liquid. The field control effects of 500 and 1000 times of Preparation Example 4, 400 and 800 times of Preparation Example 5, 500 and 1000 times of Preparation Example 6 and 1000 and 2000 times of Preparation Example 7 on rice seedling rot and seedling rot were higher than those of the binary compound agent 20% trichlorfonazole·cartap WP 2000 times and 20% bronopol·cartap WP 200 times and the control agent 20% cyproconazole·cartap WP 1000 times and 16% prochloraz·cartap WP 500 times, and the difference was significant. Due to the high effective content of cartap, the control effect of 20% bronopol·cartap WP 200 times on dry tip nematode disease reached 100%. The 500 and 1000 times liquid of Preparation Example 4; the 400 and 800 times liquid of Preparation Example 5; the 500 and 1000 times liquid of Preparation Example 6 and the 1000 and 2000 times liquid of Preparation Example 7 have higher control effects on rice stem tip nematode disease than the control agent 20% trichlorfonazole·carbamido WP 2000 times liquid and the control agent 20% cyanobacteryl·carbamido WP 1000 times liquid, and the difference is significant. The 500 and 1000 times liquid of Preparation Example 4; the 400 times liquid of Preparation Example 5; the 500 and 1000 times liquid of Preparation Example 6 have higher control effects on rice stem tip nematode disease than the control agent 16% prochloraz·carbamido WP 500 times liquid. Since the effective dosage of the insecticidal active ingredient cartap is significantly lower, the control effect of the 800-fold solution of Preparation Example 5 and the 1000- and 2000-fold solutions of Preparation Example 7 on rice stem nematode disease is lower than that of the 500-fold solution of 16% prochloraz·cartap WP (Table 3).

[0086] This shows that the pesticide composition containing chlorpyrifos, bronopol and cartap has good control effect on rice seedling rot, seedling blight and dry tip nematode disease. When the application dosage is the same, the control effect is significantly better than the control effect of the binary combination 20% chlorpyrifos·carb WP 2000 times solution and 20% bronopol·carb WP 200 times solution, and the control effect when applied at a certain safe dose is significantly better than the control effect of the conventional agents 20% cyproconazole·carb WP and 16% prochloraz·carb WP applied at the recommended dose. It reduces the use of pesticides and increases the efficacy, has high quality and low price, is environmentally friendly and safe, is of great significance for the comprehensive control of rice seedling blight and other diseases, and has significant economic, ecological and social benefits.

[0087] Table 3 Field efficacy of the preferred compositions prepared in the examples of the present invention against rice seedling rot, seedling blight and dry tip nematode disease

[0088]

[0089]

[0090] Note: Data in the same column followed by different lowercase letters indicate significant differences (P<0.05).

[0091] Field efficacy example 3: Field efficacy test of a pesticide composition containing trichlorfon, bronopol and cartap against bacterial basal rot of rice.

[0092] Experimental agent design: same as field efficacy example 1.

[0093] Experimental treatment method: The experimental site is located in the experimental field in the Agricultural Science and Technology Innovation Center Park of Zhenjiang Agricultural Science Research Institute in the Jiangsu Hilly Area of ​​Baitu Town, Jurong City, Jiangsu Province. The soil is horse liver soil with medium fertility and an organic matter content of about 1.80%. The rice variety is Yangfujing No. 8. The experiment has 13 treatments, the same as field efficacy example 1. 500g of 'Yangfujing No. 8' rice seeds are soaked in 1L of the above 13 treatments or water for 48h, and the seeds are germinated for about 48h after soaking until the buds are half a grain long and sown in a 5m×1m seedbed. Each treatment is repeated 3 times. When the seedlings are 30 days old, the seedlings of each treatment are transplanted to a 20m×2m field plot with a row spacing of about 20cm×15cm, and each treatment is randomly arranged. Fertilizer, water and other pests and diseases during the rice growth period are managed as usual. The bacterial basal rot disease rate was investigated during the heading and filling period of rice, and the control effect was calculated and analyzed by variance analysis of the control effect using the DPSv7.05 data processing system and Duncan's new multiple range method. The calculation formulas for the diseased plant rate and control effect are: diseased plant rate = number of diseased plants / total number of investigated plants × 100%; control effect = [(control diseased plant rate - treatment diseased plant rate) / control diseased plant rate] × 100%.

[0094] The test results show that the field control effects of the 12 agents on rice bacterial base rot are ranked from high to low as follows: Preparation Example 5, Preparation Example 6, Preparation Example 4, Preparation Example 7, Preparation Example 1, Preparation Example 3, Preparation Example 2, 20% bronopol·cartap WP, 20% bronopol WP, 20% trichlorfonazole·cartap WP, 28% trichlorfonazole FS and 6% cartap AS. The field control effects of Preparation Examples 1-7 on rice bacterial base rot are higher than the control effects of the binary ratio agents 20% bronopol·cartap WP and 20% trichlorfonazole·cartap WP and the control agents 28% trichlorfonazole FS, 20% bronopol WP and 6% cartap AS, and the differences are significant (Table 4).

[0095] This indicates that the pesticide composition containing trichlorfonazole, bronopol and cartap has excellent control effect on rice bacterial basal rot, and the field control effect after the three-way compound is significantly enhanced compared with the two-way compound of bronopol and cartap or trichlorfonazole and cartap and the single agent. It can be seen that the pesticide composition of the present invention has great significance for the comprehensive prevention and control of rice bacterial basal rot by reducing the amount of pesticides and increasing the efficacy.

[0096] Table 4 Field efficacy of the agents prepared in the examples of the present invention against bacterial rot of rice

[0097] Test drug Treatment dose / (μg / mL) Disease rate / (%) Prevention effect / (%) Preparation Example 5 125 0.19g 93.99a Preparation Example 6 125 0.21fg 93.35ab Preparation Example 4 125 0.24efg 92.41bc Preparation Example 7 125 0.25defg 92.09cd Preparation Example 1 125 0.28def 91.14de Preparation Example 3 125 0.29de 90.82e Preparation Example 2 125 0.31d 90.19e 20% Bronopol·Cartap WP 125 0.67c 78.80f 20% Bronopol WP 125 0.72c 77.22g 20% trichlorfonazole·carbamido WP 125 0.81b 74.37h 28% trichlorfonazole FS 125 0.85b 73.10i 6% Cartap AS 125 3.17a -0.32j CK 3.16a

[0098] Note: The treatment dosages of Preparation Examples 1-7 were calculated based on the sum of the mass concentrations of trichlorfonazole and bronopol as 125 μg / mL; the treatment dosages of 20% trichlorfonazole·cartap WP, 20% bronopol·cartap WP, 28% trichlorfonazole FS, 20% bronopol WP and 6% cartap AS were calculated based on the mass concentrations of trichlorfonazole, bronopol, trichlorfonazole, bronopol and cartap as 125 μg / mL, respectively; data in the same column marked with different lowercase letters indicate significant differences (P<0.05).

[0099] Field efficacy example 4: Field efficacy test of a composition containing a preferred ratio of trichlorfon, bronopol and cartap against bacterial basal rot of rice.

[0100] Experimental agent design: same as field efficacy example 2.

[0101] Experimental treatment method: The experimental site is located in the experimental field in the Agricultural Science and Technology Innovation Center Park of Zhenjiang Agricultural Science Research Institute in the Jiangsu Hilly Area of ​​Baitu Town, Jurong City, Jiangsu Province. The soil is horse liver soil with medium fertility and an organic matter content of about 1.80%. The rice variety is Yangfujing No. 8. The experiment has 13 treatments, the same as field efficacy embodiment 2. 500g of 'Yangfujing No. 8' rice seeds are soaked in 1L of the above 13 treatments or water for 48h, and the seeds are germinated for about 48h after soaking until the buds are half a grain long and sown in a 5m×1m seedbed. Each treatment is repeated 3 times. When the seedlings are 30 days old, the seedlings of each treatment are transplanted to a 20m×2m field plot with a row spacing of about 20cm×15cm, and each treatment is randomly arranged. Fertilizer, water and other pests and diseases during the rice growth period are managed as usual. The bacterial basal rot disease rate was investigated during the heading and filling period of rice, and the control effect was calculated and analyzed by variance analysis of the control effect using the DPSv7.05 data processing system and Duncan's new multiple range method. The calculation formulas for the diseased plant rate and control effect are: diseased plant rate = number of diseased plants / total number of investigated plants × 100%; control effect = [(control diseased plant rate - treatment diseased plant rate) / control diseased plant rate] × 100%.

[0102] The test results show that the field control effects of the 12 agent treatments on rice bacterial base rot are as follows from high to low: Preparation Example 4, 500 times liquid; Preparation Example 5, 400 times liquid; Preparation Example 6, 500 times liquid; Preparation Example 7, 1000 times liquid; Preparation Example 4, 1000 times liquid; Preparation Example 5, 800 times liquid; Preparation Example 6, 1000 times liquid; Preparation Example 7, 2000 times liquid; 20% bronopol·carb WP 200 times liquid; 20% chlorpyrifos·carb WP 2000 times liquid; 16% prochloraz·carb WP 500 times liquid; and 20% cyanoacrylate·carb WP 1000 times liquid. The field control effects of 500 and 1000 times of Preparation Example 4, 400 and 800 times of Preparation Example 5, 500 and 1000 times of Preparation Example 6 and 1000 and 2000 times of Preparation Example 7 on bacterial base rot of rice were higher than those of the binary compound agents 20% bronopol·carb WP 200 times and 20% trichlorfonazole·carb WP 2000 times and the control agents 16% prochloraz·carb WP 500 times and 20% cyproconazole·carb WP 1000 times, and the differences were significant (Table 5).

[0103] This shows that the pesticide composition containing chlorfenapyr, bronopol and cartap has excellent control effect on rice bacterial basal rot, and when the application dosage is the same, the control effect is significantly better than the control effect of the binary compound agent 20% bronopol·carb WP 200 times solution and 20% chlorfenapyr·carb WP 2000 times solution, and the control effect when applied at a certain safe dose is significantly better than the control effect of 20% cyanobacteryl·carb WP and 16% prochloraz·carb WP at the recommended dose. The pesticide composition containing chlorfenapyr, bronopol and cartap can provide more drug options for the comprehensive prevention and control of rice bacterial basal rot, reduce the use of drugs and increase the efficacy, have high quality and low price, are environmentally friendly and safe, and have good application prospects.

[0104] Table 5 Field efficacy of the preferred compositions prepared in the examples of the present invention against bacterial rot of rice

[0105]

[0106]

[0107] Note: Data in the same column followed by different lowercase letters indicate significant differences (P<0.05).

Claims

1. A pesticide composition containing trichlorfon, bronopol and cartap, characterized in that The invention comprises the following effective ingredients: trichlorfonazole, bronopol and cartap; the mass ratio of trichlorfonazole, bronopol and cartap is 1-4:1-4:1-4.

2. The pesticide composition containing trichlorfon, bronopol and cartap according to claim 1, characterized in that: The mass ratio of trichlorfon, bronopol and cartap is 1-2:1-4:1-4.

3. The pesticide composition containing trichlorfon, bronopol and cartap according to claim 2, characterized in that: The mass ratio of trichlorfon, bronopol and cartap is 1:1-4:

4.

4. The pesticide composition containing trichlorfon, bronopol and cartap according to claim 1, characterized in that Also includes adjuvants.

5. The pesticide composition containing trichlorfon, bronopol and cartap according to claim 4, characterized in that: The percentage content of the active ingredient in the pesticide composition is 10% to 48% by mass.

6. The pesticide composition containing trichlorfon, bronopol and cartap according to claim 5, characterized in that: The dosage form is wettable powder or dispersible powder for seed treatment.

7. The pesticide composition containing trichlorfon, bronopol and cartap according to claim 6, characterized in that: For wettable powders, the auxiliary agent is selected from one or more of fillers, wetting agents, dispersants, penetrants, stabilizers, and warning agents; for dispersible powders for seed treatment, the auxiliary agent is selected from one or more of fillers, wetting agents, dispersants, penetrants, film-forming agents, stabilizers, and warning agents; for wettable powders, the filler is selected from bentonite, the wetting agent is selected from sodium dodecylbenzene sulfonate, the dispersant is selected from calcium lignin sulfonate, the penetrant is selected from tridecanol polyoxyethylene ether, the stabilizer is selected from tributyl phosphate, and the warning agent is selected from Haishu Green G1; for dispersible powders for seed treatment, the filler is selected from kaolin, the wetting agent is selected from Lakai powder BX, the dispersant is selected from sodium styrene sulfonate, the penetrant is selected from sodium diethyl succinate sulfonate, the film-forming agent is selected from polyvinyl alcohol, the stabilizer is selected from isopropyl phosphate, and the warning agent is selected from carmine.

8. The pesticide composition containing trichlorfon, bronopol and cartap according to claim 7, characterized in that: In terms of mass percentage, the wettable powder comprises: 1% to 30% of trichlorfon, 1% to 22.5% of bronopol, 1.5% to 17% of cartap, 5% to 6% of wetting agent, 5% to 6% of dispersant, 0.2% to 0.5% of penetrant, 0.5% to 1% of stabilizer, 0.2% to 1% of color warning agent, and filler to make up 100%; Measured in percentage by mass, the dispersible powder for seed treatment comprises: 2%-6% of trichlorfon, 2%-6% of bronopol, 3%-10% of cartap, 5%-7% of wetting agent, 5%-7% of dispersant, 0.2%-0.5% of penetrant, 1%-2% of film-forming agent, 0.5%-1% of stabilizer, 0.2%-1% of color warning agent, and 100% of filler.

9. Use of the pesticide composition containing trichlorfon, bronopol and cartap according to any one of claims 1 to 8 in preventing and controlling rice seedling rot and / or rice seedling bakanae disease and / or rice stem tip nematode disease.

10. Use of the pesticide composition containing trichlorfon, bronopol and cartap according to any one of claims 1 to 8 in preventing and controlling bacterial basal rot of rice.

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