A liver protection compound composition, preparation method and application thereof

Abstract

The present application discloses a liver protection compound composition, a preparation method and its application, and belongs to the technical field of health food and functional food research and development. The compound composition comprises the following parts by weight: 100-350 parts of milk thistle seed oil suspension, 100-400 parts of high phospholipid krill oil, and 10-50 parts of rice bran fatty alkyl alcohol. The liver protection compound composition of the present invention has a scientific proportion of contents, and each active ingredient cooperates to sober up and protect the liver. It uses natural food raw materials, is environmentally friendly and healthy, has no toxic side effects, and is non-dependent.

Description

Technical Field

[0001] The present application belongs to the technical field of research and development of health food and functional food, and specifically relates to a liver protection compound composition, a preparation method and its application. Background Art

[0002] Alcoholic liver disease (ALD) is the leading cause of death among young adults in developed countries. Long-term excessive drinking can lead to alcohol accumulation in the body, causing varying degrees of liver damage, and ultimately leading to alcoholic liver disease. Alcoholic liver disease can be divided into alcoholic fatty liver, alcoholic hepatitis, alcoholic cirrhosis, etc., and there is a possibility that two or more lesions exist at the same time. In Western countries, alcoholic liver disease has been listed as one of the main causes of liver disease-related diseases and deaths. In my country, with the development of the economy and the improvement of people's living standards, the alcohol consumption group is becoming increasingly large, and the incidence of alcoholic liver disease is gradually increasing. The development of drugs and health foods for the prevention and treatment of alcoholic liver disease has received widespread attention from the pharmaceutical and food industries. At present, most of the drugs for the treatment of liver disease on the market are synthetic drugs, but Western medicine has many adverse reactions, high prices, and long-term use has defects such as dependence.

[0003] The liver is an important detoxification organ in the human body. The detoxification and metabolism of alcohol is mainly carried out in the liver. Long-term and large-scale intake of alcoholic beverages causes the liver to exceed its normal physiological metabolic and detoxification capacity, leading to many important physiological, biochemical, and metabolic imbalances in the body, severely damaging liver cells and causing alcoholic liver disease. The market treatment of alcoholic liver disease is mainly based on Western medicine and traditional Chinese medicine. Long-term use of drugs will increase the burden on the liver.

[0004] The Chinese patent number CN 112586698 A discloses a liver protection composition and preparation method, which includes the following raw materials: kiwi fruit powder, corn oligopeptides, artichoke powder, vitamin C, rice bran fatty alkyl alcohol, oyster peptide powder, yeast extract, D-mannitol, oligoxylose and auxiliary materials. However, the invention has too many ingredients, and long-term consumption will increase the gastrointestinal burden and cause gastrointestinal dysfunction. At the same time, the cost of the composition is relatively high.

[0005] Antarctic krill mainly live in the waters around Antarctica. They have a large amount of biological resources, are rich in nutrients and have a high content of high-quality protein. Antarctic krill oil is a kind of oil extracted from Antarctic krill. It is rich in unsaturated fatty acids, vitamins and other nutrients. It has the functions and effects of assisting in lowering blood lipids and assisting in protecting cardiovascular and cerebrovascular vessels. It can also play a role in assisting in enhancing human immunity. However, there are few reports on the liver protection effect of Antarctic krill oil. Therefore, the study of a non-toxic, natural and inexpensive liver protection compound composition containing Antarctic krill oil can provide a technical reference for research and application in this field. Summary of the invention

[0006] In order to solve the above problems, the present invention provides a liver protection compound composition, a preparation method and an application thereof. The contents of the liver protection compound composition of the present invention are scientifically proportioned, and the active ingredients synergistically relieve alcohol and protect the liver. Natural food raw materials are used, which is environmentally friendly, healthy, non-toxic, non-addictive, and has no side effects.

[0007] On one hand, the present invention provides a liver protection compound composition, which comprises the following parts by weight: 100-350 parts of milk thistle seed oil suspension, 100-400 parts of high-phospholipid krill oil, and 10-50 parts of rice bran fatty alkanol.

[0008] Another aspect of the present invention provides a method for preparing the liver protection compound composition, the preparation method comprising the following steps:

[0009] (1) washing and screening the milk thistle seeds, drying, heating to 30-80° C. to soften and squeeze, and then alkali washing, water washing, decolorizing, deodorizing, cooling, and filtering to obtain milk thistle seed oil and remaining milk thistle seed oil residue;

[0010] (2) extracting the remaining milk thistle seed oil residue after crushing with 50%-90% ethanol, vacuum filtering the supernatant to recover the solvent to obtain an extract, and mixing the extract with the milk thistle seed oil to obtain a milk thistle seed oil suspension;

[0011] (3) grinding the freeze-dried Antarctic krill with a grinder to obtain Antarctic krill powder, extracting it with a subcritical extraction method to obtain krill oil, adding anhydrous ethanol to the krill oil for re-dissolution, filtering it with a microporous filter membrane, and recovering the ethanol to obtain high-phospholipid krill oil;

[0012] (4) heating the milk thistle seed oil suspension to 50-80° C., adding rice bran fatty alcohol and stirring to dissolve, to obtain a fat mixture 1, and cooling to 30-40° C.;

[0013] (5) adding the high phospholipid krill oil to the oil mixture 1, stirring and homogenizing for 10-50 minutes to obtain the oil mixture 2;

[0014] (6) The oil mixture 2 is allowed to stand, vacuum degassed, and filtered to obtain a liver protection compound composition.

[0015] Optionally, in the subcritical extraction method of step (3), n-hexane is used as the extractant, the solid-liquid ratio of Antarctic krill powder to n-hexane is 1:3-8, and the extraction is carried out at a pressure of 0.3-0.8 MPa and a temperature of 20-50°C.

[0016] Optionally, the vacuum degassing time in step (6) is 1-5 h, the vacuum pressure is 0.1-0.3 MPa, and the temperature is 10-40°C.

[0017] In another aspect, the present invention provides a liver-protecting soft capsule, wherein the soft capsule comprises the liver-protecting compound composition or the liver-protecting compound composition prepared by the above-mentioned preparation method.

[0018] Optionally, the liver-protecting soft capsule further comprises gelatin, purified water, glycerin and sorbitol.

[0019] In another aspect, the present invention provides a liver-protecting hard capsule, wherein the hard capsule comprises the liver-protecting compound composition or the liver-protecting compound composition prepared by the above-mentioned preparation method.

[0020] Optionally, the liver protection hard capsule further comprises mono- and di-glycerol fatty acid esters and sodium caseinate.

[0021] Another aspect of the present invention provides a liver-protecting jelly, characterized in that the liver-protecting jelly comprises the above-mentioned liver-protecting compound composition or the liver-protecting compound composition prepared by the above-mentioned preparation method.

[0022] Optionally, the liver-protecting jelly further comprises sorbitol, gelatin, xanthan gum, carrageenan, agar, pectin, glycerin, potassium sorbate, and sodium hydroxymethylcellulose.

[0023] The beneficial effects of this application include but are not limited to:

[0024] 1. The liver protection compound composition provided by the present invention has a scientifically proportioned content. Antarctic krill oil can improve the bioavailability of other ingredients and cooperate with other ingredients to sober up and protect the liver;

[0025] 2. The liver protection compound composition provided by the present invention adopts natural food raw materials, which is environmentally friendly, healthy, non-toxic, non-addictive;

[0026] 3. The liver protection compound composition provided by the present invention can be made into three effective dosage forms: soft capsules, hard capsules and jelly through innovative processes;

[0027] 4. The preparation method of the liver protection compound composition provided by the present invention can maximize the effects of the raw materials themselves in sobering up, protecting the liver and improving alcoholic fatty liver;

[0028] 5. The preparation method of the present invention adopts batch raw materials and industrial equipment, has strong versatility, is easy to mass produce, and has good application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0030] Figure 1A histogram of ALT activity levels in the serum of mice in the embodiments and comparative examples of the present application;

[0031] Figure 2 The bar graph of AST activity level in the serum of mice in the embodiments and comparative examples involved in this application;

[0032] Figure 3 The figure is a bar graph showing the TG activity level in the serum of mice in the examples and comparative examples involved in the present application. DETAILED DESCRIPTION

[0033] In order to more clearly illustrate the overall concept of the present invention, a detailed description is given below by way of examples.

[0034] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Therefore, the protection scope of the present invention is not limited to the specific embodiments disclosed below.

[0035] In the present invention, unless otherwise clearly specified and limited, the first feature "on" or "under" the second feature may be that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "examples", "specific examples" or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in an appropriate manner in any one or more embodiments or examples.

[0036] Hereinafter, a liver protection compound composition, a preparation method and applications thereof of the present invention will be described in detail in conjunction with exemplary embodiments.

[0037] In an exemplary embodiment of the present invention, the liver protection compound composition can be prepared by the following method, and the specific steps are as follows:

[0038] (1) The milk thistle seeds are cleaned, screened, dried, heated to 30-80° C. for softening, pressed, and then alkali washed, washed with water, decolorized, deodorized, cooled, and filtered to obtain milk thistle seed oil and remaining milk thistle seed oil residue.

[0039] (2) extracting the remaining milk thistle seed oil residue after crushing with 50%-90% ethanol, vacuum filtering the supernatant to recover the solvent to obtain an extract, and mixing the extract with the milk thistle seed oil to obtain a milk thistle seed oil suspension.

[0040] Specifically, silymarin is an effective ingredient for protecting the liver. The silymarin can be extracted by treating the silymarin seed residue with an ethanol solution, with an extraction rate of about 1%-5%.

[0041] (3) The freeze-dried Antarctic krill is crushed with a pulverizer to obtain Antarctic krill powder, and the subcritical extraction method is used to extract to obtain shrimp oil, anhydrous ethanol is added to the shrimp oil for re-dissolution, and the ethanol is recovered after filtering through a microporous filter membrane to obtain high-phospholipid krill oil. Specifically, the subcritical extraction method uses n-hexane as an extractant, the solid-liquid ratio of Antarctic krill powder to n-hexane is 1:3-8, and the extraction is carried out under the conditions of a pressure of 0.3-0.8MPa and a temperature of 20-50°C. The phospholipid content of the high-phospholipid krill oil is 40%-80%.

[0042] Specifically, high-phospholipid krill oil has a significant liver-protecting effect. Krill oil can be effectively extracted through subcritical extraction, and the obtained krill oil has a high phospholipid content.

[0043] (4) 100-350 parts of milk thistle seed oil suspension are heated to 50-80° C., 10-50 parts of rice bran fatty alkanol are added and stirred to dissolve to obtain a fat mixture 1, and the mixture is cooled to 30-40° C.

[0044] Specifically, rice bran fatty alkyl alcohols need to be heated to dissolve, but krill oil easily loses its activity at high temperatures, so it needs to be cooled before adding krill oil.

[0045] (5) Add 100-400 parts of high-phospholipid krill oil to the oil mixture 1, stir and homogenize for 10-50 minutes to obtain the oil mixture 2.

[0046] (6) The oil mixture 2 is allowed to stand, vacuum degassed, and filtered to obtain a liver protection compound composition. Specifically, the vacuum degassing time is 1-5 hours, the vacuum pressure is 0.1-0.3 MPa, preferably, the vacuum pressure is 0.1 MPa, and the temperature is 10-40°C.

[0047] Specifically, if there are too many bubbles during the preparation process, the product filling amount will be unstable. The purpose of degassing is to stabilize the product filling amount and maintain the fluidity of the product at the temperature of this application.

[0048] Another aspect of the present invention provides a liver-protecting soft capsule, which further comprises 200-500 parts of gelatin, 200-500 parts of purified water, 100-200 parts of glycerol, and 30-80 parts of sorbitol. The specific preparation steps are as follows:

[0049] (1) 100-350 parts of milk thistle seed oil suspension are heated to 50-80° C., 10-50 parts of rice bran fatty alkanol are added and stirred to dissolve to obtain a fat mixture 1, and the mixture is cooled to 30-40° C.

[0050] (2) Add 100-400 parts of high-phospholipid krill oil, stir and homogenize for 30 minutes to obtain oil mixture 2.

[0051] (3) After the oil mixture 2 is allowed to stand, it is vacuum degassed at a vacuum pressure of 0.1-0.3 MPa and a temperature of 35° C. for 2 h, and filtered to obtain a liver protection compound composition.

[0052] (4) Add 100-200 parts of glycerol and 30-80 parts of sorbitol to 200-500 parts of purified water, dilute and stir evenly, then put the liquid and purified water into a gelatinization tank, heat and stir at a temperature of 50-75°C, then add 200-500 parts of gelatin, stir and keep warm for 30-60 minutes, wait until the gelatin is completely dissolved, mix evenly, then start the automatic degassing system for degassing, and keep the vacuum degree no more than 0.075MPa during degassing. After degassing, press the gelatin liquid and the contents into soft capsule particles, and dry them for final shaping.

[0053] In another aspect, the present invention provides a liver-protecting hard capsule, which further comprises 0.5% to 5% by mass of mono- and di-glycerol fatty acid esters and 1% to 10% by mass of sodium caseinate. The specific preparation steps are as follows:

[0054] (1) 100-350 parts of milk thistle seed oil suspension and 100-400 parts of high phospholipid krill oil are uniformly mixed to form a milk thistle seed oil and krill oil mixture.

[0055] (2) Add 0.5%-5% of mono- and di-glycerol fatty acid esters and 1%-10% of sodium caseinate to the mixture of milk thistle seed oil and krill oil, heat to 50-80° C. to dissolve, and then stir thoroughly to form a uniform mixture.

[0056] (3) homogenizing the mixture for 10-30 minutes, and then freeze-drying to form milk thistle seed oil krill microcapsule powder, with the freezing time being 1-5 hours and the drying time being 5-20 hours.

[0057] (4) Add 10-50 parts of rice bran fatty alkyl alcohol to the milk thistle seed oil and krill oil microcapsule powder, mix well and load into hard capsule particles.

[0058] Another aspect of the present invention provides a liver-protecting jelly, characterized in that the liver-protecting jelly comprises the above-mentioned liver-protecting compound composition or the liver-protecting compound composition prepared by the above-mentioned preparation method. It also comprises 500-1000 parts of sorbitol, 100-300 parts of gelatin, 100-250 parts of xanthan gum, 100-200 parts of carrageenan, 50-200 parts of agar, 50-100 parts of pectin, 10-50 parts of glycerol, 0.5-5 parts of potassium sorbate, and 0.1-0.5 parts of sodium hydroxymethylcellulose. The specific preparation steps are as follows:

[0059] (1) Mix gelatin and xanthan gum in a mass ratio of 1:1-5, add 10-20 times of purified water, heat to 10-80°C, stir and dissolve for 10-50 minutes, and cool to 20-50°C to obtain a gelatin-xanthan gum mixture.

[0060] (2) 10-50 parts of rice bran fatty alkyl alcohol are added to 100-350 parts of milk thistle seed oil suspension, heated to 65° C. to dissolve, cooled to 40° C., and then 100-400 parts of high phospholipid krill oil, 10-50 parts of glycerol and gelatin xanthan gum mixture are added, and homogenized for 10 minutes to form a uniform krill oil emulsion.

[0061] (3) After mixing carrageenan, agar and pectin in a mass ratio of 2:1:1, add 5-10 times of sorbitol, first fully swell in 80-100 times of purified water at 15°C for 30 minutes, then boil the gel at 100°C for 20 minutes, and cool to 40°C for use.

[0062] (4) Add krill oil emulsion, sodium hydroxymethyl cellulose and potassium sorbate to the above glue solution, stir evenly, put into a mold, seal it and cool it to room temperature.

[0063] The liver protection compound composition of the present invention can be used to prepare liver protection soft capsules, liver protection hard capsules and liver protection jelly, and can also be used in other foods and health products.

[0064] Example 1

[0065] This example uses the above preparation method to prepare a liver-protecting soft capsule, and the specific steps are as follows:

[0066] (1) Wash and screen the milk thistle seeds, dry them, heat them to 60° C. to soften them, squeeze them, and then wash them with alkali, wash them with water, decolorize them, deodorize them, cool them, and filter them to obtain milk thistle seed oil and residual milk thistle seed oil residue. Use 80% ethanol to extract the crushed residual milk thistle seed oil residue, vacuum filter the supernatant to recover the solvent, and obtain an extract. The extract is mixed with the milk thistle seed oil to obtain a milk thistle seed oil suspension.

[0067] (2) The freeze-dried Antarctic krill is crushed with a pulverizer to obtain Antarctic krill powder, and n-hexane is used as an extractant. The solid-liquid ratio of Antarctic krill powder to n-hexane is 1:7. The extraction is carried out at a pressure of 0.5 MPa and a temperature of 30° C. for 2 h to obtain shrimp oil. Anhydrous ethanol is added to the shrimp oil for re-dissolution, and the ethanol is recovered after filtering through a microporous filter membrane to obtain high-phospholipid krill oil.

[0068] (3) The 280 parts of the milk thistle seed oil suspension were heated to 70° C., 20 parts of rice bran fatty alcohol were added and stirred to dissolve, to obtain a fat mixture 1, and the mixture was cooled to 40° C.

[0069] (4) Add 200 parts of high-phospholipid krill oil to the oil mixture 1, stir and homogenize for 30 minutes to obtain the oil mixture 2.

[0070] (5) The oil mixture 2 was allowed to stand, and vacuum degassed for 2 h at a vacuum pressure of 0.1 MPa and a temperature of 35° C., and filtered to obtain a liver protection compound composition.

[0071] (6) Add 130 parts of glycerol and 60 parts of sorbitol to 300 parts of purified water, dilute and stir evenly, then put the liquid and purified water into a gelatinization tank, heat and stir at 75°C, then add 300 parts of gelatin, stir and keep warm for 60 minutes, wait until the gelatin is completely dissolved, mix evenly, then start the automatic degassing system for degassing, and keep the vacuum degree no more than 0.075 MPa during degassing. After degassing, press the gelatin liquid and the contents into soft capsule particles with a specification of 500 mg / granule, shape and dry to obtain liver-protecting soft capsules.

[0072] Example 2

[0073] In this embodiment, another liver-protecting soft capsule is prepared by the above-mentioned preparation method, and the specific steps are as follows:

[0074] (1) Wash and screen the milk thistle seeds, dry them, heat them to 55° C. to soften them, squeeze them, and then wash them with alkali, wash them with water, decolorize them, deodorize them, cool them, and filter them to obtain milk thistle seed oil and residual milk thistle seed oil residue. Use 70% ethanol to extract the crushed residual milk thistle seed oil residue, vacuum filter the supernatant to recover the solvent, and obtain an extract. The extract is mixed with the milk thistle seed oil to obtain a milk thistle seed oil suspension.

[0075] (2) The freeze-dried Antarctic krill is crushed with a pulverizer to obtain Antarctic krill powder, and n-hexane is used as an extractant. The solid-liquid ratio of Antarctic krill powder to n-hexane is 1:5. The extraction is carried out at a pressure of 0.3 MPa and a temperature of 35° C. for 3 h to obtain shrimp oil. Anhydrous ethanol is added to the shrimp oil for re-dissolution, and the ethanol is recovered after filtering through a microporous filter membrane to obtain high-phospholipid krill oil.

[0076] (3) The 300 parts of milk thistle seed oil suspension were heated to 70° C., 30 parts of rice bran fatty alcohol were added and stirred to dissolve, to obtain a fat mixture 1, and the mixture was cooled to 40° C.

[0077] (4) Add 300 parts of high-phospholipid krill oil to the oil mixture 1, stir and homogenize for 30 minutes to obtain the oil mixture 2.

[0078] (5) The oil mixture 2 was allowed to stand, and vacuum degassed for 2 h at a vacuum pressure of 0.1 MPa and a temperature of 35° C., and filtered to obtain a liver protection compound composition.

[0079] (6) Add 130 parts of glycerol and 60 parts of sorbitol to 300 parts of purified water, dilute and stir evenly, then put the liquid and purified water into a gelatinization tank, heat and stir at 75°C, then add 300 parts of gelatin, stir and keep warm for 60 minutes, wait until the gelatin is completely dissolved, mix evenly, then start the automatic degassing system for degassing, and keep the vacuum degree no more than 0.075 MPa during degassing. After degassing, press the gelatin liquid and the contents into soft capsule particles with a specification of 500 mg / granule, shape and dry to obtain liver-protecting soft capsules.

[0080] Example 3

[0081] In this embodiment, another liver-protecting soft capsule is prepared by the above-mentioned preparation method, and the specific steps are as follows:

[0082] (1) Wash and screen the milk thistle seeds, dry them, heat them to 70° C. to soften them, squeeze them, and then wash them with alkali, wash them with water, decolorize them, deodorize them, cool them, and filter them to obtain milk thistle seed oil and residual milk thistle seed oil residue. Use 60% ethanol to extract the crushed residual milk thistle seed oil residue, vacuum filter the supernatant to recover the solvent, and obtain an extract. The extract is mixed with the milk thistle seed oil to obtain a milk thistle seed oil suspension.

[0083] (2) The freeze-dried Antarctic krill is crushed with a pulverizer to obtain Antarctic krill powder, and n-hexane is used as an extractant. The solid-liquid ratio of Antarctic krill powder to n-hexane is 1:3. The extraction is carried out at a pressure of 0.3 MPa and a temperature of 40° C. for 3 h to obtain shrimp oil. Anhydrous ethanol is added to the shrimp oil for re-dissolution, and the ethanol is recovered after filtering through a microporous filter membrane to obtain high-phospholipid krill oil.

[0084] (3) The 320 parts of the milk thistle seed oil suspension were heated to 70° C., 40 parts of rice bran fatty alcohol were added and stirred to dissolve, to obtain a fat mixture 1, and the mixture was cooled to 40° C.

[0085] (4) Add 350 parts of high-phospholipid krill oil to the oil mixture 1, stir and homogenize for 30 minutes to obtain the oil mixture 2.

[0086] (5) The oil mixture 2 was allowed to stand, and vacuum degassed for 2 h at a vacuum pressure of 0.1 MPa and a temperature of 35° C., and filtered to obtain a liver protection compound composition.

[0087] (6) Add 130 parts of glycerol and 60 parts of sorbitol to 300 parts of purified water, dilute and stir evenly, then put the liquid and purified water into a gelatinization tank, heat and stir at 75°C, then add 300 parts of gelatin, stir and keep warm for 60 minutes, wait until the gelatin is completely dissolved, mix evenly, then start the automatic degassing system for degassing, and keep the vacuum degree no more than 0.075 MPa during degassing. After degassing, press the gelatin liquid and the contents into soft capsule particles with a specification of 500 mg / granule, shape and dry to obtain liver-protecting soft capsules.

[0088] Example 4

[0089] This embodiment adopts the above preparation method to prepare a liver protection hard capsule, and the specific steps are as follows:

[0090] (1) Wash and screen the milk thistle seeds, dry them, heat them to 55° C. to soften them, squeeze them, and then wash them with alkali, wash them with water, decolorize them, deodorize them, cool them, and filter them to obtain milk thistle seed oil and residual milk thistle seed oil residue. Use 80% ethanol to extract the crushed residual milk thistle seed oil residue, vacuum filter the supernatant to recover the solvent, and obtain an extract. The extract is mixed with the milk thistle seed oil to obtain a milk thistle seed oil suspension.

[0091] (2) The freeze-dried Antarctic krill is crushed with a pulverizer to obtain Antarctic krill powder, and n-hexane is used as an extractant. The solid-liquid ratio of Antarctic krill powder to n-hexane is 1:8. The extraction is carried out at a pressure of 0.3 MPa and a temperature of 35° C. for 3 h to obtain shrimp oil. Anhydrous ethanol is added to the shrimp oil for re-dissolution, and the ethanol is recovered after filtering through a microporous filter membrane to obtain high-phospholipid krill oil.

[0092] (3) 340 parts of the milk thistle seed oil suspension and 150 parts of the high-phospholipid krill oil were uniformly mixed to form a milk thistle seed oil-krill oil mixture.

[0093] (4) Add 2.5% of mono- and di-glycerol fatty acid esters and 4% of sodium caseinate to the mixture of milk thistle seed oil and krill oil, heat to 70° C. to dissolve, and then stir thoroughly to form a uniform mixture.

[0094] (5) The mixture was homogenized for 10 minutes, and then freeze-dried to form milk thistle seed oil krill microcapsule powder. The freezing time was 5 hours and the drying time was 14 hours.

[0095] (6) Add 10 parts of rice bran fatty alkyl alcohol to the milk thistle seed oil and krill oil microcapsule powder, mix well and put into No. 0 hard capsule particles (content is about 500 mg) to obtain liver protection hard capsules.

[0096] Example 5

[0097] This embodiment adopts the above preparation method to prepare a liver-protecting jelly, and the specific steps are as follows:

[0098] (1) Wash and screen the milk thistle seeds, dry them, heat them to 70° C. to soften them, squeeze them, and then wash them with alkali, wash them with water, decolorize them, deodorize them, cool them, and filter them to obtain milk thistle seed oil and residual milk thistle seed oil residue. Use 90% ethanol to extract the crushed residual milk thistle seed oil residue, vacuum filter the supernatant to recover the solvent, and obtain an extract. The extract is mixed with the milk thistle seed oil to obtain a milk thistle seed oil suspension.

[0099] (2) The freeze-dried Antarctic krill is crushed with a pulverizer to obtain Antarctic krill powder, and n-hexane is used as an extractant. The solid-liquid ratio of Antarctic krill powder to n-hexane is 1:7. The extraction is carried out at a pressure of 0.3 MPa and a temperature of 35° C. for 3 h to obtain shrimp oil. Anhydrous ethanol is added to the shrimp oil for re-dissolution, and the ethanol is recovered after filtering through a microporous filter membrane to obtain high-phospholipid krill oil.

[0100] (3) Mix 170 parts of gelatin and 170 parts of xanthan gum evenly, add 20 times of purified water, heat to 60°C and stir to dissolve for 20 minutes, and cool to 40°C to obtain a gelatin-xanthan gum mixture.

[0101] (4) 10 parts of rice bran fatty alkyl alcohols were added to 100 parts of milk thistle seed oil suspension and heated to 65°C to dissolve, then cooled to 40°C and added with 100 parts of high phospholipid krill oil, 35 parts of glycerol and gelatin xanthan gum mixture, and homogenized for 10 minutes to form a uniform krill oil emulsion.

[0102] (5) After 170 parts of carrageenan, 90 parts of agar, and 65 parts of pectin are mixed evenly, 900 parts of sorbitol are added, and the mixture is first fully swollen in 100 times purified water at 15°C for 30 minutes, then boiled at 100°C for 20 minutes, and cooled to 40°C for use.

[0103] (6) Add krill oil emulsion, 0.2 parts of sodium hydroxymethylcellulose, and 3 parts of potassium sorbate to the above-mentioned glue, stir evenly, put into a mold, seal, and cool to room temperature to obtain liver-protecting jelly containing about 500 mg of krill oil emulsion per piece.

[0104] Comparative Example 1

[0105] In this comparative example, a comparative liver protection soft capsule was prepared by the same preparation method as in Example 1, except that step (1) was omitted and commercially available silybum seed oil was used instead of the silybum seed oil suspension prepared in the present application.

[0106] Comparative Example 2

[0107] In this comparative example, a comparative liver protection soft capsule was prepared by the same preparation method as in Example 1, except that step (2) was omitted and commercially available krill oil was used instead of the high phospholipid krill oil prepared in the present application.

[0108] Comparative Example 3

[0109] In this comparative example, another liver-protecting soft capsule was prepared by the same preparation method as in Example 1, except that rice bran fatty alkyl alcohol was not added in step (3).

[0110] Test 1: Human hangover test

[0111] 1.1. The soft capsules of Examples 1-3, the hard capsules of Example 4, the jelly of Example 5 and the soft capsules of Comparative Examples 1-3 were tested on human subjects.

[0112] 1.2. Experimenters: 48 people were selected based on their drinking habits, drinking amount, drinking status, etc., including 24 males and 24 females.

[0113] 1.3 Experimental methods:

[0114] Three males and three females formed a group and were divided into eight groups. One hour after lunch that afternoon, the first group took 2 soft capsules of Example 1, the second group took 2 soft capsules of Example 2, the third group took 2 soft capsules of Example 3, the fourth group took 2 hard capsules of Example 4, the fifth group took 2 jellies of Example 5, the sixth group took 2 soft capsules of Comparative Example 1, the seventh group took 2 soft capsules of Comparative Example 2, and the eighth group took 2 soft capsules of Comparative Example 3. The females drank 180 mL and the males drank 360 mL of alcohol (shochu). After 2 hours, the alcohol concentration in the breath and the hangover questionnaire were measured.

[0115] Table 1 Alcohol concentration in breath

[0116] Group Corresponding embodiment Alcohol test after 2 hours Group 1 Example 1 Alcohol was detected in both persons Group 2 Example 2 Alcohol was detected in all three people Group 3 Example 3 Alcohol was detected in all three people Group 4 Example 4 Alcohol was detected in all three people Group 5 Example 5 Alcohol was detected in all 4 people Group 6 Comparative Example 1 Alcohol was detected in all 5 people Group 7 Comparative Example 2 Alcohol was detected in all 5 people Group 8 Comparative Example 3 Alcohol was detected in all 6 people

[0117] Table 2 Hangover Questionnaire

[0118]

[0119]

[0120] As shown in Table 1 and Table 2, it can be seen from the comparison that the soft capsules prepared in Examples 1-3, the hard capsules prepared in Example 4, and the liver-protecting jelly prepared in Example 5 can effectively degrade the breath alcohol content, and have a good alleviating effect on symptoms such as drowsiness and headache after drinking. In particular, the soft capsules prepared in Example 1 have the best effect. By comparison, it can be seen that, compared with Example 1, Comparative Example 1 lacks the step of preparing the milk thistle seed oil suspension, but is replaced by milk thistle seed oil available on the market. Compared with Example 1, Comparative Example 2 lacks the step of preparing high phospholipid krill oil, but is replaced by krill oil available on the market. The soft capsules finally prepared are poor in the effect of degrading the breath alcohol content and relieving the symptoms after drinking, indicating that the milk thistle seed oil suspension and high phospholipid krill oil obtained by the preparation method of the present application have a significant effect on improving alcoholic fatty liver. Comparative Example 3 Compared with Example 1, no rice bran fatty alkanol was added, and the obtained soft capsules were less effective in degrading the breath alcohol content and relieving the symptoms after drinking, indicating that rice bran fatty alkanol can significantly reduce the cholesterol content in the blood, relieve hangovers, and significantly improve hangover reactions as well as symptoms such as listlessness, drowsiness, and sticky feeling in the mouth after waking up from alcohol.

[0121] Test 2: Liver protection animal experiment

[0122] 2.1 Research methods: Kunming mice were raised and blood and tissues were collected for testing after dissection.

[0123] 2.2 Grouping: divided into blank control group, model group, Example 1 low dose, Example 1 group high dose, Example 2 group high dose, Example 3 group high dose, Example 4 group high dose, Example 5 group high dose, Comparative Example 1 group high dose, Comparative Example 2 group high dose, Comparative Example 3 group high dose. Among them, the blank control group did not receive any treatment, and the model group was an acute drunkenness model.

[0124] 2.3 An acute intoxication model was established by intragastric administration of alcohol, and the biochemical indices in the serum of each group were measured to evaluate the protective effect on acute liver injury.

[0125] 2.4 Experimental results:

[0126] Table 3. Activity levels of factors in mouse serum

[0127]

[0128]

[0129] From Table 3 and Figure 1-3It can be seen that compared with the blank control group, the serum factors ALT, AST, and TG of the mice in the model group increased, and decreased after administration. Among them, the high dose of Example 1 group was better than other groups and had the best effect, indicating that the high dose of Example 1 group had a significant protective effect on acute alcohol liver injury in mice.

[0130] The above is only the embodiment of the present application, and the protection scope of the present application is not limited by these specific embodiments, but is determined by the claims of the present application. For those skilled in the art, the present application can have various changes and variations. Any modification, equivalent replacement, improvement, etc. made within the technical ideas and principles of the present application should be included in the protection scope of the present application.

Claims

1. A liver protection compound composition, characterized in that: The compound composition is composed of the following parts by mass: 280 parts of milk thistle seed oil suspension, 200 parts of high-phospholipid krill oil, and 20 parts of rice bran fatty alkanol; The method for preparing the compound composition comprises the following steps: (1) washing and screening milk thistle seeds, drying, heating to 60° C. to soften, pressing, and then alkali washing, water washing, decolorizing, deodorizing, cooling, and filtering to obtain milk thistle seed oil and remaining milk thistle seed oil residue; (2) extracting the remaining milk thistle seed oil residue after crushing with 80% ethanol, vacuum filtering the supernatant to recover the solvent to obtain an extract, and mixing the extract with the milk thistle seed oil to obtain a milk thistle seed oil suspension; (3) grinding the freeze-dried Antarctic krill with a grinder to obtain Antarctic krill powder, extracting it with a subcritical extraction method to obtain krill oil, adding anhydrous ethanol to the krill oil for re-dissolution, filtering it with a microporous filter membrane, and recovering the ethanol to obtain high-phospholipid krill oil; (4) heating the milk thistle seed oil suspension to 70° C., adding rice bran fatty alkanol and stirring to dissolve, to obtain a fat mixture 1, and cooling to 40° C.; (5) adding the high phospholipid krill oil to the oil mixture 1, stirring and homogenizing for 30 minutes to obtain the oil mixture 2; (6) allowing the oil mixture 2 to stand, vacuum degassing, and filtering to obtain a liver protection compound composition; The subcritical extraction method in step (3) uses n-hexane as the extractant, the solid-liquid ratio of Antarctic krill powder to n-hexane is 1:7, and the extraction is performed under the conditions of a pressure of 0.5 MPa and a temperature of 30° C.; The vacuum degassing time in step (6) is 2 hours, the vacuum pressure is 0.1 MPa, and the temperature is 35°C.

2. A liver-protecting soft capsule, characterized in that: The soft capsule comprises the liver protecting compound composition according to claim 1.

3. The liver-protecting soft capsule according to claim 2, characterized in that: The liver-protecting soft capsule further comprises gelatin, purified water, glycerol and sorbitol.

4. A liver-protecting hard capsule, characterized in that: The hard capsule comprises the liver protecting compound composition according to claim 1.

5. The liver-protecting hard capsule according to claim 4, characterized in that: The liver-protecting hard capsule also includes mono- and di-glycerol fatty acid esters and sodium caseinate.

6. A liver-protecting jelly, characterized in that: The liver-protecting jelly comprises the liver-protecting compound composition as claimed in claim 1.

7. The liver-protecting jelly according to claim 6, characterized in that: The liver-protecting jelly also includes sorbitol, gelatin, xanthan gum, carrageenan, agar, pectin, glycerol, potassium sorbate and sodium hydroxymethylcellulose.

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