Method for preventing and treating flue-cured tobacco virus disease

Abstract

The present application discloses a method for preventing and treating flue-cured tobacco virus disease, belonging to the field of plant protection. The method comprises (1) prevention: applying agents during the seedling stage, before transplanting, after transplanting, and during the field stage of tobacco growth; during the seedling stage: applying guanidine-copper acetate before thinning, replanting, and weeding; applying amino oligosaccharides and antiviral microbial agents before sealing the trays; applying cinclostrobin acetate solution after sealing the trays and before cutting the leaves; applying ningnanmycin and amino oligosaccharides before and after transplanting; applying insecticides mixed with plant resistance inducers, antiviral microbial agents, trace elements, and foliar fertilizers from one week after transplanting the seedlings to the stage of clustering; (2) treatment of the disease. The method prevents flue-cured tobacco virus disease by applying antiviral agents early in the whole growth stage of tobacco; and combining the antiviral agents with other specific agents to improve tobacco plant resistance, reduce the incidence of viral diseases, and improve the cure rate of viral diseases.

Description

Technical Field

[0001] The present application belongs to the field of plant protection and relates to a method for preventing and treating flue-cured tobacco virus diseases. Background Art

[0002] Flue-cured tobacco virus disease is a disease that affects the entire growth period. To achieve better results, comprehensive measures should be taken from multiple aspects, such as selecting disease-resistant varieties, cultivating disease-free and virus-free seedlings, crop rotation, drug prevention, etc., to improve the resistance of tobacco plants.

[0003] Flue-cured tobacco virus diseases affect tobacco throughout its entire growth period. Currently, 16 tobacco virus diseases have been identified, with TMV, CMV, and PVY being the primary causes of tobacco mosaic virus. In most areas, viral diseases occur as a combination of several viruses, including cucumber mosaic virus (CMV) and common mosaic virus (TMV).

[0004] Current viral disease control agents, such as Ningnanmycin and guanidine hydrochloride, have an antiviral efficacy of between 20% and 60%. This is less than ideal. Therefore, a supplementary method is needed to further enhance the efficacy of viral disease control agents against flue-cured tobacco viruses.

[0005] The current prevention measures for tobacco virus diseases are relatively simple in terms of medication and are used too late, resulting in insignificant prevention effects. Indiscriminate use of drugs, drug damage, and excessive pesticide residues often occur. Summary of the Invention

[0006] In order to solve the above problems, a method for preventing and controlling flue-cured tobacco virus diseases is provided. This method prevents flue-cured tobacco virus diseases by applying antiviral agents at an early stage during the entire growth stage of flue-cured tobacco; and the antiviral agents are applied in combination with other specific agents to improve the resistance of tobacco plants, reduce the incidence of viral diseases and the cure rate of viral diseases.

[0007] The specific technical solutions of this application are as follows:

[0008] The present application provides a method for preventing and treating flue-cured tobacco virus diseases, comprising:

[0009] Step 1: Prevention

[0010] The pesticides were applied during the seedling stage, before transplanting, after transplanting, and during the clustering stage of tobacco in the field.

[0011] Seedling stage: Apply guanidine copper acetate before thinning, replanting, and weeding; apply amino oligosaccharides and antiviral microbial agents before closing the trays; apply cinclostrobin acetate solution after closing the trays and before cutting the leaves;

[0012] The medication is used 1 to 2 times in each stage;

[0013] Before and after transplanting: apply a mixture of Ningnanmycin, amino oligosaccharides, and imidacloprid; apply the mixture once before and after transplanting.

[0014] Apply insecticide from one week after transplanting the seedlings to the stage of clustering, and mix it with plant resistance inducers, antiviral microbial agents, trace elements, and foliar fertilizers. Apply the medicine 1-3 times.

[0015] From one week after the seedlings are transplanted to the clustering stage, the insecticides include imidacloprid and thiamethoxam; the plant resistance inducers include amino oligosaccharides, lentinan, and oligosaccharide-chain proteins; the antiviral microbial agent includes Rhodopseudomonas palustris PSB-S; the trace elements include sugar alcohol zinc trace element water-soluble fertilizer; the foliar fertilizer includes potassium dihydrogen phosphate, sugar alcohol zinc, and zinc sulfate;

[0016] Step 2: Treatment of the disease.

[0017] Optionally, during the seedling period of step 1, the active ingredient content of morphoguanidine copper acetate is 18-22%, and the application amount is 145-155 g / mu; the active ingredient content of amino oligosaccharides is 4-6%, and the application amount is 70-80 ml / mu; the antiviral microbial agent is prodigiosin, which is diluted 120-150 times and then sprayed, and the application amount is 200-300 ml / mu; the active ingredient content of cinclostrobin acetate is 7-9%, and it is diluted 3000 times and then applied, and the application amount is 70-80 ml / mu.

[0018] Preferably, the active ingredient content of the morphoguanidine copper acetate is 20%, and the application amount is 150g / mu;

[0019] The active ingredient content of the amino oligosaccharide is 5%, and the application amount is 75 ml / mu;

[0020] The active ingredient content of the octamidine acetate is 8%.

[0021] Optionally, before cutting the leaves, the cutting tools are disinfected with a 1500-fold dilution of octamidine acetate having an active ingredient content of 7-9%. Preferably, the active ingredient content of the octamidine acetate is 8%.

[0022] Optionally, before and after transplanting out of the greenhouse in step 1, the active ingredient content of Ningnanmycin is 7-9%, the solution is diluted 1600 times, and the application amount is 200-300 ml / mu; the active ingredient content of amino oligosaccharides is 4-6%, and the application amount is 70-80 ml / mu; the active ingredient content of imidacloprid is 65-75%, and the application amount is 1.5-2.5 g / mu.

[0023] Preferably, the active ingredient content of Ningnanmycin is 8%; the active ingredient content of amino oligosaccharides is 5%, and the application amount is 75 ml / mu; the active ingredient content of imidacloprid is 70%, and the application amount is 1.5-2.5 g / mu.

[0024] Optionally, apply the drug twice from one week after transplanting the seedlings in step 1 to the middle of the clustering stage;

[0025] The first application is of imidacloprid with an active ingredient content of 70% at a rate of 3-4 g / mu; the second application is of thiamethoxam with an active ingredient content of 25% at a rate of 4-8 g / mu; when applying, imidacloprid or thiamethoxam is mixed with amino oligosaccharides, Rhodopseudomonas palustris PSB-S, sugar alcohol zinc trace element water-soluble fertilizer, and potassium dihydrogen phosphate respectively.

[0026] Among them, the active ingredient content of amino oligosaccharides is 4-7%, and the application amount is 75-100 ml / mu; the active ingredient content of potassium dihydrogen phosphate used in small clusters is 0.1%, and the application amount is 15-20 grams / 15 kilograms of water. After large clusters, the active ingredient content is gradually increased to 0.3%, and the application amount is 40-50 grams / 15 kilograms of water; the sugar alcohol zinc trace element water-soluble fertilizer is diluted 1200-1500 times, and the application amount is 10-12.5 ml / 15 kilograms of water; the concentration of the bacterial agent of Pseudomonas palustris PSB-S is 210-230 million CFU / ml, and the application amount is 180-240 ml / mu; the method of applying the agent is to spray on the front and back sides of the leaves.

[0027] Preferably, the active ingredient content of the amino oligosaccharide is 5%; the active ingredient content of potassium dihydrogen phosphate used in small clumps is 0.1%, and the application amount is 15 grams / 15 kilograms of water. After large clumps, the active ingredient content is gradually increased to 0.3%, and the application amount is 45 grams / 15 kilograms of water; the bacterial agent concentration of Rhodopseudomonas palustris PSB-S is 200 million CFU / ml.

[0028] Optionally, the viral diseases targeted by the onset treatment include tobacco cucumber mosaic virus disease, tobacco common mosaic virus disease, and potato virus Y disease.

[0029] When the virus disease is tobacco cucumber mosaic virus or tobacco common mosaic virus, apply the following agents: 300-400 times diluted guanidine hydrochloride with an active ingredient content of 20%; 300 times diluted liquid nitrogen; 800-1200 times diluted pyraclostrobin with an active ingredient content of 25%; 1000 times diluted potassium dihydrogen phosphate and 1000-2000 times diluted brassinolide;

[0030] When the virus disease is potato virus Y, apply the following agents:

[0031] Virus inhibitors: 300-400 times diluted copper succinate and morpholine guanidine with an active ingredient content of 18-22%; 1200-1600 times diluted ningnanmycin with an active ingredient content of 7-9%; 800-1200 times diluted morphoguanidine and copper acetate with an active ingredient content of 18-22%; use the three alternately;

[0032] Foliar fertilizer: zinc sulfate foliar spray concentration is 0.1-0.2%; liquid nitrogen diluted 300-400 times;

[0033] New broad-spectrum fungicide: pyraclostrobin with an active ingredient content of 22-27%, 30-40 ml / mu;

[0034] Plant growth regulator: Brassinolide diluted 1000-2000 times, or S-absorbent;

[0035] Additive: Jijian, diluted 1500-2000 times;

[0036] Application method: Spray on both sides of the leaves.

[0037] The flue-cured tobacco virus diseases in this application include tobacco cucumber mosaic virus disease, tobacco common mosaic virus disease, and potato virus Y disease.

[0038] The present application also provides the application of the above method in improving the stress resistance of flue-cured tobacco plants.

[0039] Those skilled in the art will know that flue-cured tobacco virus diseases can occur in various growth stages of flue-cured tobacco. The present application applies different agents to flue-cured tobacco in various growth stages to improve the prevention of virus diseases.

[0040] In practice, the present application has obtained agents that are effective in preventing viral diseases in different growth stages; different agents applied in each growth stage effectively increase the incidence of viruses in each growth stage.

[0041] The beneficial effects of this application include but are not limited to:

[0042] 1. The combined preventive and therapeutic measures adopted by this application. This application improves the immunity of flue-cured tobacco against viral diseases by applying specific agents at various growth stages of flue-cured tobacco, effectively reducing the incidence of viruses at each growth stage. Furthermore, the use of specific agents in conjunction with antiviral agents can synergize with the antiviral agents to further improve the cure rate for flue-cured tobacco viral diseases, surpassing the currently effective antiviral agents (such as Ningnanmycin and Morphoguanidine hydrochloride) which have an antiviral effect of only 20%-60%.

[0043] 2. The applicant applied morphoguanidine-copper acetate before thinning, replanting, and weeding, which was more beneficial for preventing and treating viral diseases than applying it after thinning, replanting, and weeding. This is because the amino acid structure in the plant is more active before thinning, replanting, and weeding, and the amino and carboxyl groups in the amino acids can form hydrogen bonds with morphoguanidine-copper acetate molecules, thereby helping the plant better absorb and transport the pesticide. In this way, the goal of enhancing the antiviral ability of flue-cured tobacco is achieved.

[0044] Furthermore, the applicants have discovered that combining the application of morphoguanidine-copper acetate before seedling thinning, seedling replacement, and weeding with the subsequent application of amino oligosaccharides and prodigiosin before closing the seedling trays during the seedling raising period can further enhance the prevention and control of flue-cured tobacco virus diseases. This is because these two drug applications exhibit a synergistic effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0046] Figure 1 For this application, pictures of flue-cured tobacco plants at the growth and maturity stages are provided;

[0047] Figure 2 Another picture of the growth and maturity stage of flue-cured tobacco for this application;

[0048] Figure 3 Another picture of the growth and maturity stage of flue-cured tobacco for this application;

[0049] Figure 4 This is a picture of a single flue-cured tobacco plant at the mature stage of growth for this application;

[0050] Figure 5 This is a picture of flue-cured tobacco in the growth and maturity stage of comparative example 1 of this application;

[0051] Figure 6 This is a picture of flue-cured tobacco in the growth and maturity stage of comparative example 2 of this application;

[0052] Figure 7 This is a picture of flue-cured tobacco in the growth and maturity stage of comparative example 3 of this application;

[0053] Figure 8 This is a picture of the flue-cured tobacco in comparative example 6 of this application during its growth and maturity stage. DETAILED DESCRIPTION

[0054] The present invention is described in detail below with reference to the following examples, but the present invention is not limited to these examples. Reagents not specifically described in the present invention are all commercially available products.

[0055] Regarding the attached drawings, Figure 1For this application, pictures of flue-cured tobacco plants at the growth and maturity stages are provided; Figure 2 Another picture of the growth and maturity stage of flue-cured tobacco for this application; Figure 3 Another picture of the growth and maturity stage of flue-cured tobacco for this application; Figure 4 This is a picture of a single flue-cured tobacco plant at the mature stage of growth for this application;

[0056] Figure 5 This is a picture of flue-cured tobacco in comparative example 1 of the present application at the mature stage of growth (showing common mosaic virus disease), with the leaf edges curled downward, and the leaves wrinkled and twisted and deformed; Figure 6 This is the comparative example 2 of the present application. The flue-cured tobacco in the mature stage of growth (cucumber mosaic) has blister spots and curled leaf edges; Figure 7 This is a picture of the comparative example 3 of this application showing lightning-shaped necrosis "oak leaf pattern" in the mature stage of flue-cured tobacco (cucumber mosaic disease); Figure 8 This is a picture of flue-cured tobacco in comparative example 6 of this application at the mature stage. Several weeks after systemic infection with potato virus Y, tiny brown to white necrotic spots appeared on the leaves.

[0057] Example 1

[0058] Step 1: Prevention

[0059] The pesticide was applied during the seedling stage, before transplanting, after transplanting, and during the clustering stage (the ratio of tobacco plant width to height is about 2:1, and the plant shape is approximately spherical, which is called the clustering stage) of flue-cured tobacco growth.

[0060] (1) Seedling stage: Before thinning, replanting, and weeding, apply 20% morphoguanidine copper acetate at a rate of 150 g / mu. Before sealing the pots, apply 5% amino oligosaccharide at a rate of 75 ml / mu and spray with 200-300 ml / mu of prodigiosin diluted 120-150 times. After sealing the pots and before cutting leaves, apply 8% cinclorac-acetate solution diluted 3000 times at a rate of 70-80 ml / mu. Among them, leaf cutting tools should be disinfected with 8% cinclorac-acetate diluted 1500 times. Apply the medicine once at each stage.

[0061] (2) Before and after transplanting out of the greenhouse (transplanting to the field): apply 8% of Ningnanmycin, diluted 1600 times; 5% of amino oligosaccharide, applied at a rate of 75 ml / mu; 70% of imidacloprid, applied at a rate of 2 g / mu; apply the medicine once before and after transplanting.

[0062] (3) Apply the medicine twice from one week after transplanting the seedlings to the stage of clustering: the first application is imidacloprid with an active ingredient content of 70% at a rate of 3 g / mu; the second application is thiamethoxam with an active ingredient content of 25% at a rate of 5 g / mu; when applying imidacloprid or thiamethoxam, they are mixed with plant attractant - amino oligosaccharide, microbial agent Rhodopseudomonas palustris PSB-S, trace element - sugar alcohol zinc trace element water-soluble fertilizer, and foliar fertilizer - potassium dihydrogen phosphate;

[0063] Among them, the active ingredient content of amino oligosaccharides is 5%, and the application rate is 80 ml / mu; the active ingredient content of potassium dihydrogen phosphate is 0.1% when the plants are in small clusters, and the application rate is 15 grams / 15 kg of water. After the plants are in large clusters, the active ingredient content is gradually increased to 0.3%, and the application rate is 45 grams / 15 kg of water; the zinc saccharide is diluted about 1300 times, and the application rate is 12 ml / 15 kg of water; the concentration of the bacterial agent for Rhodopseudomonas palustris PSB-S is 200 million CFU / ml, and the application rate is 200 ml / mu;

[0064] The method of applying the agent is to spray on both sides of the leaves.

[0065] Step 2: Treatment of the disease

[0066] When the virus disease is tobacco cucumber mosaic virus disease or tobacco common mosaic virus disease, the following agents are applied: 20% active ingredient content of morpholinoguanidine hydrochloride diluted 300 times, liquid nitrogen diluted 300 times, 25% active ingredient content of pyraclostrobin diluted 1000 times, 3% active ingredient content of potassium dihydrogen phosphate diluted 300-500 times, and 1500 times dilution of brassinolide;

[0067] When the virus disease is potato virus Y, apply the following agents:

[0068] Virus inhibitors: 20% active ingredient content of copper succinate·morpholine guanidine, diluted 300 times; 8% active ingredient content of ningnanmycin, diluted 1500 times; 20% active ingredient content of morphoguanidine·copper acetate, diluted 1000 times; use the three alternately;

[0069] Foliar fertilizer: zinc sulfate foliar spray concentration is 0.15%; liquid nitrogen diluted 300 times;

[0070] New broad-spectrum fungicide: pyraclostrobin with an active ingredient content of 25%, applied at a rate of 30 ml / mu;

[0071] Plant growth regulator: Brassinolide diluted 2000 times;

[0072] The brassinolide can be any one of propionylbrassinolide, 28-homobrassinolide, 24-mixed epibrassinolide (24-epibrassinolide, tripebrassinolide), and 28-epihomobrassinolide. In this embodiment, propionylbrassinolide is used, and the same applies hereinafter.

[0073] Additive: Jijian, diluted 2000 times;

[0074] Application method: Spray on both sides of the leaves.

[0075] Example 2

[0076] Step 1: Prevention

[0077] The pesticide was applied during the seedling stage, before transplanting, after transplanting, and during the clustering stage (the ratio of tobacco plant width to height is about 2:1, and the plant shape is approximately spherical, which is called the clustering stage) of flue-cured tobacco growth.

[0078] (1) Seedling stage: Before thinning, replanting, and weeding, apply 20% morphoguanidine copper acetate at a rate of 150 g / mu. Before sealing the pots, apply 5% amino oligosaccharide at a rate of 75 ml / mu and spray with 200-300 ml / mu of prodigiosin diluted 120-150 times. After sealing the pots and before cutting leaves, apply 8% cinclorac-acetate solution diluted 3000 times at a rate of 70-80 ml / mu. Among them, leaf cutting tools should be disinfected with 8% cinclorac-acetate diluted 1500 times. Apply the medicine once at each stage.

[0079] (2) Before and after transplanting: apply Ningnanmycin with an active ingredient content of 8%, diluted 1600 times; amino oligosaccharide with an active ingredient content of 5%, applied at a rate of 75 ml / mu; imidacloprid with an active ingredient content of 70%, applied at a rate of 2 g / mu; apply the medicine once before and after transplanting.

[0080] Apply the drug twice from one week after transplanting the seedlings to the stage of clustering: the first application contains 70% imidacloprid at a rate of 3g / mu; when applying imidacloprid, mix it with the plant inducer - amino oligosaccharide, the microbial agent - Rhodopseudomonas palustris PSB-S, the trace element - sugar alcohol zinc trace element water-soluble fertilizer, and the foliar fertilizer - potassium dihydrogen phosphate;

[0081] The second application of thiamethoxam with an active ingredient content of 25% was conducted at a rate of 5 g / mu. Thiamethoxam was mixed with a plant inducer - lentinan, a microbial agent - Rhodopseudomonas palustris PSB-S, a trace element - sugar alcohol zinc water-soluble fertilizer, and a foliar fertilizer - sugar alcohol zinc.

[0082] Among them, the effective ingredient content of lentinan and amino oligosaccharides is 5%, and the application rate is 80 ml / mu. The effective ingredient content of potassium dihydrogen phosphate is 0.1% when the plants are in small clusters, and the application rate is 15 grams / 15 kg of water. After the plants are in large clusters, the effective ingredient content is gradually increased to 0.3% and the application rate is 45 grams / 15 kg of water. The zinc glycoside is diluted about 1300 times and the application rate is 12 ml / 15 kg of water. The concentration of the bacterial agent for Rhodopseudomonas palustris PSB-S is 200 million CFU / ml, and the application rate is 200 ml / mu.

[0083] The method of applying the agent is to spray on the front and back sides of the leaves;

[0084] Step 2: Treatment of the disease

[0085] When the virus disease is tobacco cucumber mosaic virus disease or tobacco common mosaic virus disease, the following agents are applied: 20% active ingredient content of morpholinoguanidine hydrochloride diluted 300 times, liquid nitrogen diluted 300 times, 25% active ingredient content of pyraclostrobin diluted 1000 times, 3% active ingredient content of potassium dihydrogen phosphate diluted 300-500 times, and 1500 times dilution of brassinolide;

[0086] When the virus disease is potato virus Y, apply the following agents:

[0087] Virus inhibitors: 20% active ingredient content of copper succinate·morpholine guanidine, diluted 300 times; 8% active ingredient content of ningnanmycin, diluted 1500 times; 20% active ingredient content of morphoguanidine·copper acetate, diluted 1000 times; use the three alternately;

[0088] Foliar fertilizer: zinc sulfate foliar spray concentration is 0.15%; liquid nitrogen diluted 300 times;

[0089] New broad-spectrum fungicide: pyraclostrobin with an active ingredient content of 25%, applied at a rate of 30 ml / mu;

[0090] Plant growth regulator: Brassinolide diluted 2000 times;

[0091] Additive: Jijian, diluted 2000 times;

[0092] Application method: Spray on both sides of the leaves.

[0093] Comparative Example 1:

[0094] The difference from Example 1 is that, in step 1, during the seedling raising period, morphoguanidine-copper acetate is applied after thinning the seedlings, replanting the seedlings, and weeding. The rest is the same as in Example 1.

[0095] Comparative Example 2:

[0096] Prodigiosin was applied before thinning, replanting and weeding, and amino oligosaccharides and morphoguanidine-copper acetate were applied before sealing the tray.

[0097] Comparative Example 3

[0098] The difference from Example 1 is that the antiviral microbial agent used in step 1 during the seedling raising period is different, namely, Trichoderma harzianum. The rest is the same as Example 1.

[0099] The difference between Comparative Example 4 and Example 1 is that the dosage of the drug used in the first step before and after transplanting is different.

[0100] In step 1 of this comparative example, before and after transplanting out of the greenhouse, 1600 times diluted ningnanmycin with an active ingredient content of 12%, 75 ml / mu of amino oligosaccharide with an active ingredient content of 5%, and 2 g / mu of imidacloprid with an active ingredient content of 62% were applied; the drugs were applied once before and after transplanting; the rest were the same as in Example 1.

[0101] The difference between Comparative Example 5 and Example 1 is that in step 1, a different plant resistance inducer, chlorpyrifos, is applied from one week after the seedlings are transplanted to the cluster stage. The rest is the same as in Example 1.

[0102] The difference between Comparative Example 6 and Example 1 is that, in step 2, when the viral disease is potato virus Y, the viral inhibitor applied is:

[0103] A solution of copper succinate·morpholineguanidine with an active ingredient content of 20% was diluted 300-500 times; a solution of morphoguanidine·copper acetate with an active ingredient content of 20% was diluted 1000 times; the two were used alternately; the rest was the same as in Example 1.

[0104] Virus inhibitors: 20% active ingredient content of copper succinate·morpholine guanidine, diluted 300 times; 8% active ingredient content of ningnanmycin, diluted 1500 times; 20% active ingredient content of morphoguanidine·copper acetate, diluted 1000 times; use the three alternately;

[0105] The seedling stage and field management conditions of the above-mentioned examples and comparative examples were the same. The incidence rates of tobacco cucumber mosaic virus, tobacco common mosaic virus, and potato virus Y during the vigorous growth period to the mature growth period of the flue-cured tobacco in the above-mentioned examples and comparative examples were observed and statistically analyzed, and the results are shown in Table 1 below.

[0106] Table 1 Effects of different medication measures on the prevention and treatment of viral diseases

[0107]

[0108]

[0109] As shown in Table 1, Example 2 had the lowest virus incidence rate and the best control effect. Compared with Example 2, Example 1 had a poorer virus control effect. The reason for this is that in Example 2, potassium dihydrogen phosphate and zinc glycosides were used alternately as foliar fertilizers, and amino oligosaccharides and lentinan were used alternately as plant resistance agents, which further enhanced the immune capacity of flue-cured tobacco (resistance to viral infection).

[0110] The difference in virus incidence between Comparative Example 1 and the embodiment shows that applying guanidine copper acetate before thinning, replanting, and weeding is more conducive to the prevention and control of viral diseases. The reason may be that the amino acid structure in the plant body is more active before thinning, replanting, and weeding, and the amino and carboxyl groups in the amino acids can form hydrogen bonds with guanidine copper acetate molecules, thereby helping the plants to better absorb and transport pesticides.

[0111] The difference in virus incidence between Comparative Example 2 and the Example demonstrates that combining the application of morphoguanidine-copper acetate before seedling thinning, seedling replacement, and weeding with the subsequent application of oligosaccharides and prodigiosin before closing the seedling trays during the seedling raising period further enhances the prevention and control of flue-cured tobacco virus diseases. This further demonstrates a synergistic effect between these two drug application methods.

[0112] The difference in virus incidence between Comparative Example 3 and the embodiment shows that among the antiviral microbial agents, Rhodopseudomonas palustris PSB-S has a better effect of improving plant immunity and preventing and controlling viral diseases when applied in combination with other agents in this application.

[0113] The difference in viral incidence between Comparative Example 4 and the Example demonstrates that a higher active ingredient content of the antiviral agent is not necessarily better. The selection of the active ingredient content of the antiviral agent Ningnanmycin before and after transplanting requires confirmation in conjunction with the combined effects of other agents. In practice, the applicant has determined the optimal concentration of Ningnanmycin and imidacloprid for optimal control of flue-cured tobacco virus disease.

[0114] The difference in viral disease incidence between Comparative Example 5 and Example 1 shows that when a single inducer is used, amino oligosaccharides have a better effect in preventing and treating viral diseases than other plant inducers. Further, according to Example 2, it can be seen that the effect is better when amino oligosaccharides and lentinan are used alternately.

[0115] The difference in virus incidence between Comparative Example 6 and the embodiment shows that the combined use of 20% copper succinate·morpholine guanidine, 8% ningnanmycin, and 20% morphoguanidine·copper acetate can significantly reduce the treatment rate of viral diseases compared to the combined use of 20% copper succinate·morpholine guanidine and 20% morphoguanidine·copper acetate, especially for potato virus Y disease of flue-cured tobacco. It has a very significant therapeutic effect, can inhibit the large-scale spread of potato virus Y disease, and reduce the infection rate between tobacco plants.

[0116] Table 2 Effects of different pesticide application measures on flue-cured tobacco growth

[0117] project Plant height / cm Effective leaf / piece Yield per mu (kg) Output value per mu (yuan) Example 1 110 20 160 5200 Example 2 120 22 170 5500 Comparative Example 1 106 19 154 4600 Comparative Example 2 104 20 152 4600 Comparative Example 3 107 18 158 4900 Comparative Example 4 106 17 162 5000 Comparative Example 5 102 19 165 5100 Comparative Example 6 104 19 160 5000

[0118] As shown in Table 2, compared to Comparative Examples 1-6, the flue-cured tobacco in the Examples showed better growth and higher economic benefits. This is because, on the one hand, the Examples had better viral disease prevention and control effects and lower viral morbidity, which in turn provided better soil and other growth conditions / environment for the tobacco plants (lower probability of soil infection by viruses), leading to better growth. On the other hand, the various agents applied at different times in the Examples further promoted the growth of the tobacco plants.

[0119] The foregoing is merely an embodiment of the present application, and the scope of protection of the present application is not limited by these specific embodiments, but is determined by the claims of the present application. For those skilled in the art, the present application may have various modifications and variations. Any modifications, equivalent substitutions, improvements, etc. made within the technical ideas and principles of the present application should be included in the scope of protection of the present application.

Claims

1. A method for preventing and treating flue-cured tobacco virus diseases, characterized in that: include: Step 1: Prevention The pesticides were applied during the seedling stage, before transplanting, after transplanting, and during the clustering stage of tobacco in the field. (1) Seedling stage: Apply guanidine copper acetate before thinning, replanting, and weeding; apply amino oligosaccharides and antiviral microbial agents before closing the trays; apply cinclostrobin acetate solution after closing the trays and before cutting the leaves; The medication is used 1 to 2 times in each stage; (2) Before and after transplanting: apply a mixture of Ningnanmycin, amino oligosaccharides, and imidacloprid; apply the mixture once before and after transplanting; (3) Apply insecticides from one week after transplanting to the clustering stage, and mix with plant resistance inducers, antiviral microbial agents, trace elements, and foliar fertilizers. Apply 1-3 times. From one week after the seedlings are transplanted to the stage of clustering, the insecticides include imidacloprid and thiamethoxam; the plant inducers include amino oligosaccharides, lentinan, and oligosaccharide-chain protein; the antiviral microbial agent includes Rhodopseudomonas palustris PSB-S; the trace elements include sugar alcohol zinc trace element water-soluble fertilizer; the foliar fertilizer includes potassium dihydrogen phosphate, sugar alcohol zinc, and zinc sulfate; During the seedling period of step 1, the active ingredient content of morphoguanidine copper acetate is 18-22%, and the application amount is 145-155 g / mu; the active ingredient content of amino oligosaccharides is 4-6%, and the application amount is 70-80 ml / mu; the antiviral microbial agent is prodigiosin, which is diluted 120-150 times and then sprayed at an application amount of 200-300 ml / mu; the active ingredient content of cinclostrobin acetate is 7-9%, and it is diluted 3000 times and then applied at an application amount of 70-80 ml / mu; Step 2: Treatment of the disease.

2. The method according to claim 1, characterized in that The active ingredient content of the morphoguanidine copper acetate is 20%, and the application amount is 150g / mu; The active ingredient content of the amino oligosaccharide is 5%, and the application amount is 75 ml / mu; The active ingredient content of the octamidine acetate is 8%.

3. The method according to claim 1, characterized in that Before cutting leaves, disinfect the cutting tools with a 1500-fold dilution of 7-9% oxadiazine acetate.

4. The method according to claim 3, characterized in that The active ingredient content of the octamidine acetate is 8%.

5. The method according to claim 1, wherein In step 1, before and after transplanting out of the greenhouse, the active ingredient content of Ningnanmycin is 7-9%, diluted 1600 times, and the application amount is 200-300 ml / mu; the active ingredient content of amino oligosaccharides is 4-6%, and the application amount is 70-80 ml / mu; the active ingredient content of imidacloprid is 65-75%, and the application amount is 1.5-2.5g / mu.

6. The method according to claim 5, characterized in that The active ingredient content of Ningnanmycin is 8%; the active ingredient content of amino oligosaccharides is 5%, and the application amount is 75 ml / mu; the active ingredient content of imidacloprid is 70%, and the application amount is 1.5-2.5g / mu.

7. The method according to claim 1, characterized in that Apply the medicine twice from one week after transplanting the seedlings in step 1 to the middle of the clustering stage; The first application is imidacloprid with an active ingredient content of 70% and an application amount of 3-4g / mu; the second application is thiamethoxam with an active ingredient content of 25% and an application amount of 4-8g / mu; when applying imidacloprid or thiamethoxam, they are mixed with amino oligosaccharides, Rhodopseudomonas palustris PSB-S, sugar alcohol zinc trace element water-soluble fertilizer, and potassium dihydrogen phosphate respectively.

8. The method according to claim 7, characterized in that The active ingredient content of amino oligosaccharides is 4-7%, and the application amount is 75-100 ml / mu; the active ingredient content of potassium dihydrogen phosphate used in small clusters is 0.1%, and the application amount is 15-20 g / 15 kg of water. After large clusters, the active ingredient content is gradually increased to 0.3%, and the application amount is 40-50 g / 15 kg of water; the sugar alcohol zinc trace element water-soluble fertilizer is diluted 1200-1500 times, and the application amount is 10-12.5 ml / 15 kg of water; the concentration of the bacterial agent of Pseudomonas palustris PSB-S is 210-230 million CFU / ml, and the application amount is 180-240 ml / mu; the method of applying the agent is to spray on the front and back sides of the leaves.

9. The method according to claim 8, characterized in that The active ingredient content of the amino oligosaccharide is 5%; the active ingredient content of potassium dihydrogen phosphate used in small clumps is 0.1%, and the application amount is 15 grams per 15 kilograms of water. After large clumps, the active ingredient content is gradually increased to 0.3%, and the application amount is 45 grams per 15 kilograms of water; the bacterial agent concentration of Pseudomonas palustris PSB-S is 200 million CFU / ml.

10. The method according to claim 1, characterized in that The viral diseases targeted by the onset treatment include tobacco cucumber mosaic virus disease, tobacco common mosaic virus disease, and potato virus Y disease; When the virus disease is tobacco cucumber mosaic virus or tobacco common mosaic virus, apply the following agents: 300-400 times diluted guanidine hydrochloride with an active ingredient content of 20%; 300 times diluted liquid nitrogen; 800-1200 times diluted pyraclostrobin with an active ingredient content of 25%; 1000 times diluted potassium dihydrogen phosphate, 1000-2000 times diluted brassinolide; When the virus disease is potato virus Y, apply the following agents: Virus inhibitors: 300-400 times diluted copper succinate-morpholine guanidine with an active ingredient content of 18-22%; 1200-1600 times diluted ningnanmycin with an active ingredient content of 7-9%; 800-1200 times diluted morphoguanidine-copper acetate with an active ingredient content of 18-22%; use the three alternately; Foliar fertilizer: zinc sulfate foliar spray concentration is 0.1-0.2%; liquid nitrogen diluted 300-400 times; New broad-spectrum fungicide: pyraclostrobin with an active ingredient content of 22-27%, 30-40 ml / mu; Plant growth regulator: Brassinolide diluted 1000-2000 times, or S-absorbent; Additive: Jijian, diluted 1500-2000 times; Application method: Spray on both sides of the leaves.

11. The method according to claim 1, wherein The flue-cured tobacco virus diseases include tobacco cucumber mosaic virus disease, tobacco common mosaic virus disease, and potato virus Y disease.

12. Use of the method according to claim 1 in improving the stress resistance of flue-cured tobacco plants.

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