Instant polygonatum and preparation method thereof

Abstract

The invention relates to the field of health food, in particular to an instant polygonatum and a preparation method thereof. The instant polygonatum is prepared from the following raw materials, measured by weight: 220-250 parts of polygonatum fermentation liquid, 1-5 parts of chitosan, 0.1-1 part of citric acid, 60-80 parts of microcrystalline cellulose, 30-40 parts of starch, 60-80 parts of maltodextrin, 0.1-0.5 parts of sodium trimetaphosphate and an appropriate amount of solvent. The instant polygonatum prepared by the invention is edible for people, can improve human physique, enhance immunity level, increase disease resistance and delay aging.

Description

Technical Field

[0001] The invention relates to the field of health food, in particular to an instant polygonatum odoratum and a preparation method thereof. Background Art

[0002] Polygonatum odoratum is a traditional Chinese medicine in my country, known as the Taoist fairy grass. It is mild in nature, sweet in taste, enters the spleen, lung, and kidney meridians, has the effects of tonifying the spleen and lungs, nourishing yin and moistening dryness, and is suitable for treating spleen and stomach qi deficiency, fatigue and weakness, and insufficient stomach yin. It has significant effects in health care products because Polygonatum odoratum has a good effect in promoting human health.

[0003] Raw Polygonatum has a numbing feeling on the tongue and is highly irritating to the throat. The skin of people who come into contact with raw Polygonatum and its juice will feel itchy, and the smell of it for a long time will feel irritating. The ancients used the "nine steaming and nine processing" method to process Polygonatum to remove irritation and side effects and increase its tonic effect, but the "nine steaming and nine processing" method is inefficient and time-consuming, labor-intensive and energy-consuming. Fermentation can degrade important plant tissue cells or macromolecules, increase the content of active ingredients in traditional Chinese medicine, and even produce new therapeutic effects. At present, there is little research on the fermentation process of Polygonatum, and making fermented Polygonatum into health products has considerable market prospects. Summary of the invention

[0004] Purpose of the invention: In view of the above technical problems, the present invention provides an instant polygonatum and a preparation method thereof.

[0005] The technical solutions adopted are as follows:

[0006] An instant polygonatum odoratum is prepared from the following raw materials in parts by weight:

[0007] 220-250 parts of polygonatum fermentation liquid, 1-5 parts of chitosan, 0.1-1 parts of citric acid, 60-80 parts of microcrystalline cellulose, 30-40 parts of starch, 60-80 parts of maltodextrin, 0.1-0.5 parts of sodium trimetaphosphate and an appropriate amount of solvent.

[0008] Furthermore, the preparation method of the polygonatum fermentation product is as follows:

[0009] Add raw polygonatum powder into deionized water, soak it fully, add compound enzyme for enzymolysis to obtain enzymolysis product, inoculate compound bacterial agent into the enzymolysis product for fermentation, filter after fermentation, collect and inactivate the filtrate to obtain polygonatum fermentation liquid.

[0010] Furthermore, the complex enzyme comprises cellulase, hemicellulase and papain.

[0011] Furthermore, the weight ratio of the cellulase, hemicellulase and papain is 1-5:1-5:1-5.

[0012] Furthermore, the dosage of the complex enzyme is 1-3% of the weight of the polygonatum sibiricum powder.

[0013] Furthermore, the composite bacterial agent includes Aspergillus oryzae, Bacillus subtilis, Saccharomyces cerevisiae and Weissella sinensis.

[0014] Furthermore, the inoculation amount of the composite bacterial agent is 0.1-0.5% of the weight of the polygonatum sibiricum powder.

[0015] Furthermore, the solvent is ethanol.

[0016] The present invention also provides a method for preparing instant polygonatum:

[0017] After premixing the Polygonatum sibiricum fermentation liquid with the solvent, chitosan and citric acid are added and mixed, followed by adding microcrystalline cellulose, starch, maltodextrin and sodium trimetaphosphate. After fully stirring, the resulting mixture is poured into a mold, pre-frozen and then freeze-dried at a negative pressure.

[0018] Furthermore, the pre-freezing temperature is -40 to -20°C, the pre-freezing time is 10 to 30 hours, the low-temperature freeze-drying temperature is -60 to -50°C, and the vacuum degree of the low-temperature freeze-drying is 0.1 to 1×10 -4 Pa, low temperature freeze drying time is 10-30h.

[0019] Beneficial effects of the present invention:

[0020] The invention provides an instant polygonatum, which is processed by a combined process of enzymolysis and fermentation to better retain the active ingredients of the raw polygonatum and has good antioxidant properties; chitosan molecular chains are rich in alkaline -NH2 groups, and citric acid is rich in acidic -COOH groups; electrostatic action between the chitosan molecular chains forms aerogel, thereby making the prepared instant polygonatum have better taste and preservation effects, and has a certain encapsulation effect on the active ingredients of the polygonatum; sodium trimetaphosphate and starch are added to form a composite, thereby improving stability, texture and taste; the instant polygonatum prepared by the invention can be eaten by people, can improve human physique, improve immunity, increase disease resistance and delay aging. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a physical picture of the instant polygonatum prepared in Example 1 of the present invention. DETAILED DESCRIPTION

[0022] If the specific conditions are not specified in the examples, the experiments were carried out under conventional conditions or the conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments is not specified, they are all conventional products that can be purchased commercially. The techniques not mentioned in the present invention are all referenced to the prior art. Unless otherwise specified, the following examples and comparative examples are parallel experiments, using the same processing steps and parameters.

[0023] Embodiment 1:

[0024] A preparation method of instant polygonatum:

[0025] 500 g of raw polygonatum powder was added to 5 L of deionized water, stirred for 30 min and then allowed to stand and soak for 30 min, then a composite enzyme consisting of 3.5 g of cellulase, 4 g of hemicellulase and 3 g of papain was added, and enzymolysis was performed at 50 ° C for 1 h to obtain an enzymolysis product, Aspergillus oryzae, Bacillus subtilis, Saccharomyces cerevisiae and Weissella sinus were mixed in a weight ratio of 0.5:2:1:1 to obtain a composite bacterial agent, and the composite bacterial agent was inoculated into the enzymolysis product for fermentation. The inoculation amount of the composite bacterial agent was 1:1. 0.25% of the weight of the refined powder, the fermentation temperature was 35 ° C, and the continuous fermentation was filtered after 10 days. The filtrate was collected and inactivated to obtain the Polygonatum sibiricum fermentation liquid. 240g of Polygonatum sibiricum fermentation liquid was pre-mixed with 50ml of ethanol, and then 5g of chitosan and 1g of citric acid were added and stirred again. Subsequently, 65g of microcrystalline cellulose, 35g of corn starch, 70g of maltodextrin and 0.25g of sodium trimetaphosphate were added. The mixture obtained after sufficient stirring was poured into a mold, pre-frozen at -30 ° C for 24h, and then frozen at 1×10 -4 It can be freeze-dried at -60℃ under negative pressure of 1.5 Pa for 24 hours.

[0026] Embodiment 2:

[0027] A preparation method of instant polygonatum:

[0028] 500 g of raw polygonatum powder was added to 5 L of deionized water, stirred for 30 min, and then allowed to stand and soak for 30 min. A composite enzyme consisting of 3.5 g of cellulase, 4 g of hemicellulase and 3 g of papain was added. After enzymolysis at 50 ° C for 1 h, an enzymolysis product was obtained. Aspergillus oryzae, Bacillus subtilis, Saccharomyces cerevisiae and Weissella sinus were mixed in a weight ratio of 0.5:2:1:1 to obtain a composite bacterial agent. The composite bacterial agent was inoculated into the enzymolysis product for fermentation. The inoculation amount of the composite bacterial agent was 1:1. 0.5% by weight of polygonatum powder, the fermentation temperature is 35 ° C, and the continuous fermentation is filtered after 10 days. The filtrate is collected and inactivated to obtain the polygonatum fermentation liquid. 250g of polygonatum fermentation liquid is mixed with 50ml of ethanol in advance, and then 5g of chitosan and 1g of citric acid are added and stirred again. Then 80g of microcrystalline cellulose, 40g of corn starch, 80g of maltodextrin and 0.25g of sodium trimetaphosphate are added. The mixture obtained after sufficient stirring is poured into a mold, pre-frozen at -30 ° C for 24h, and then frozen at 1×10 -4 It can be freeze-dried at -60℃ under negative pressure of 1.5 Pa for 24 hours.

[0029] Embodiment 3:

[0030] A preparation method of instant polygonatum:

[0031] 500 g of raw polygonatum powder was added to 5 L of deionized water, stirred for 30 min, and then allowed to stand and soak for 30 min. A composite enzyme consisting of 3.5 g of cellulase, 4 g of hemicellulase and 3 g of papain was added. After enzymolysis at 50 ° C for 1 h, an enzymolysis product was obtained. Aspergillus oryzae, Bacillus subtilis, Saccharomyces cerevisiae and Weissella sinus were mixed in a weight ratio of 0.5:2:1:1 to obtain a composite bacterial agent. The composite bacterial agent was inoculated into the enzymolysis product for fermentation. The inoculation amount of the composite bacterial agent was 1:1. 0.1% by weight of polygonatum powder, the fermentation temperature is 35 ° C, and the continuous fermentation is filtered after 10 days. The filtrate is collected and inactivated to obtain the polygonatum fermentation liquid. 220g of polygonatum fermentation liquid is pre-mixed with 50ml of ethanol, and then 5g of chitosan and 1g of citric acid are added and stirred again. Then 60g of microcrystalline cellulose, 30g of corn starch, 60g of maltodextrin and 0.25g of sodium trimetaphosphate are added. The mixture obtained after sufficient stirring is poured into a mold, pre-frozen at -30 ° C for 24h, and then frozen at 1×10 -4 It can be freeze-dried at -60℃ under negative pressure of 1.5 Pa for 24 hours.

[0032] Embodiment 4:

[0033] A preparation method of instant polygonatum:

[0034] 500 g of raw polygonatum powder was added to 5 L of deionized water, stirred for 30 min, and then allowed to stand and soak for 30 min. A composite enzyme consisting of 3.5 g of cellulase, 4 g of hemicellulase and 3 g of papain was added. After enzymolysis at 50 ° C for 1 h, an enzymolysis product was obtained. Aspergillus oryzae, Bacillus subtilis, Saccharomyces cerevisiae and Weissella sinus were mixed in a weight ratio of 0.5:2:1:1 to obtain a composite bacterial agent. The composite bacterial agent was inoculated into the enzymolysis product for fermentation. The inoculation amount of the composite bacterial agent was 1:1. 0.5% by weight of polygonatum powder, the fermentation temperature is 35 ° C, and the continuous fermentation is filtered after 10 days. The filtrate is collected and inactivated to obtain the polygonatum fermentation liquid. 220g of polygonatum fermentation liquid is pre-mixed with 50ml of ethanol, and then 5g of chitosan and 1g of citric acid are added and stirred again. Then 80g of microcrystalline cellulose, 30g of corn starch, 80g of maltodextrin and 0.25g of sodium trimetaphosphate are added. The mixture obtained after sufficient stirring is poured into a mold, pre-frozen at -30 ° C for 24h, and then frozen at 1×10 -4 It can be freeze-dried at -60℃ under negative pressure of 1.5 Pa for 24 hours.

[0035] Embodiment 5:

[0036] A preparation method of instant polygonatum:

[0037] 500 g of raw polygonatum powder was added to 5 L of deionized water, stirred for 30 min, and then allowed to stand and soak for 30 min. A composite enzyme consisting of 3.5 g of cellulase, 4 g of hemicellulase and 3 g of papain was added. After enzymolysis at 50 ° C for 1 h, an enzymolysis product was obtained. Aspergillus oryzae, Bacillus subtilis, Saccharomyces cerevisiae and Weissella sinus were mixed in a weight ratio of 0.5:2:1:1 to obtain a composite bacterial agent. The composite bacterial agent was inoculated into the enzymolysis product for fermentation. The inoculation amount of the composite bacterial agent was 1:1. 0.2% by weight of polygonatum powder, the fermentation temperature is 35 ° C, and the continuous fermentation is filtered after 10 days. The filtrate is collected and inactivated to obtain the polygonatum fermentation liquid. 250g of polygonatum fermentation liquid is mixed with 50ml of ethanol in advance, and then 5g of chitosan and 1g of citric acid are added and stirred again. Then 70g of microcrystalline cellulose, 35g of corn starch, 60g of maltodextrin and 0.25g of sodium trimetaphosphate are added. The mixture obtained after sufficient stirring is poured into a mold, pre-frozen at -30 ° C for 24h, and then frozen at 1×10 -4 It can be freeze-dried at -60℃ under negative pressure of 1.5 Pa for 24 hours.

[0038] Comparative Example 1:

[0039] The method is substantially the same as Example 1, except that Aspergillus oryzae is not added.

[0040] Comparative Example 2:

[0041] The method is substantially the same as Example 1, except that Bacillus subtilis is not added.

[0042] Comparative Example 3:

[0043] The method is substantially the same as Example 1, except that no brewer's yeast is added.

[0044] Comparative Example 4:

[0045] The method is substantially the same as Example 1, except that Weissella sinensis is not added.

[0046] Determination of active ingredient content:

[0047] The Polygonatum cyrtonema fermented broth prepared in Examples 1 to 5 of the present invention and Comparative Examples 1 to 4 were respectively freeze-dried to constant weight and then the active ingredient content was determined. The polysaccharide content was determined by the phenol-sulfuric acid method; the reducing sugar was determined according to GB / T5009.7-2008, and the antioxidant activity was determined by the DPPH free radical scavenging rate method.

[0048] The test results are shown in Table 1 below, with the active ingredient content in g / 100g dry product:

[0049] Table 1:

[0050]

[0051] As can be seen from Table 1 above, the active ingredients retained in the Polygonatum sibiricum fermentation liquid prepared by the method of the present invention are large, have good antioxidant properties, can be made into health products for human consumption, can improve human physique, enhance immunity, increase disease resistance, and delay aging.

[0052] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An instant polygonatum, characterized in that: It is prepared from the following raw materials in parts by weight: 220-250 parts of polygonatum fermented liquid, 1-5 parts of chitosan, 0.1-1 parts of citric acid, 60-80 parts of microcrystalline cellulose, 30-40 parts of starch, 60-80 parts of maltodextrin, 0.1-0.5 parts of sodium trimetaphosphate, and an appropriate amount of solvent; The preparation method of the polygonatum fermentation product is as follows: The raw polygonatum powder is added into deionized water, and after being fully soaked, a composite enzyme is added for enzymolysis to obtain an enzymolysis product, and a composite bacterial agent is inoculated into the enzymolysis product for fermentation. After the fermentation is completed, the mixture is filtered, and the filtrate is collected and inactivated to obtain the polygonatum fermentation liquid; The complex enzyme comprises cellulase, hemicellulase and papain; The weight ratio of the cellulase, hemicellulase and papain is 1-5:1-5:1-5; The dosage of the complex enzyme is 1-3% of the weight of the polygonatum powder; The composite bacterial agent is composed of Aspergillus oryzae, Bacillus subtilis, Saccharomyces cerevisiae and Weissella sinensis in a weight ratio of 0.5:2:1:1; The inoculation amount of the composite bacterial agent is 0.1-0.5% of the weight of the polygonatum sibiricum powder; The solvent is ethanol; The preparation method of the instant polygonatum odoratum is as follows: After premixing the Polygonatum sibiricum fermented liquid with the solvent, chitosan and citric acid are added and mixed, followed by adding microcrystalline cellulose, starch, maltodextrin and sodium trimetaphosphate, and the mixture obtained after sufficient stirring is poured into a mold, pre-frozen and then freeze-dried at low temperature under negative pressure; The pre-freezing temperature is -40 to -20°C, the pre-freezing time is 10 to 30 hours, the low-temperature freeze-drying temperature is -60 to -50°C, and the vacuum degree of low-temperature freeze-drying is 0.1 to 1×10 -4 Pa, low temperature freeze drying time is 10-30h.

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