A prescription for treating ulcerative colitis and its application

Abstract

The invention belongs to the field of medical technology, and specifically relates to a prescription for treating ulcerative colitis and its application. The prescription comprises rhododendron australis, cardamom and galangal. The prescription and the prescription extract and medicine based on the prescription can effectively restore the intestinal length of the body, protect the integrity of the intestinal barrier of the body, regulate the imbalance of the intestinal acid-base homeostasis of the body, inhibit the death of intestinal epithelial cells of the body and reduce the level of ROS in the intestinal tract of the body, thereby achieving the purpose of treating ulcerative colitis.

Description

Technical Field

[0001] The invention belongs to the technical field of medicines, and in particular relates to a prescription for treating ulcerative colitis and an application thereof. Background Art

[0002] Ulcerative colitis (UC) is a chronic nonspecific inflammatory disease of the colon and rectum, with lesions limited to the mucosa and submucosa of the large intestine. The lesions are mostly located in the sigmoid colon and rectum, and can also extend to the descending colon or even the entire colon. The main symptoms of ulcerative colitis include persistent diarrhea of ​​varying severity, intermittent bloody stools, abdominal pain and systemic symptoms, which may last from weeks to years. In addition, symptoms such as mucus and blood in the stool, abdominal distension, and fever may also occur.

[0003] At present, the most common treatment for UC is drug therapy, including glucocorticoids, aminosalicylic acid, immunosuppressants and biological agents, but many drugs are limited in clinical application. For example, long-term use of prednisone or dexamethasone hormone maintenance cannot prevent UC recurrence, and may even bring a series of problems such as liver and kidney toxicity, drug dependence and repeated attacks after drug withdrawal. In addition, some biological agents are expensive and difficult for ordinary patients to afford. Therefore, seeking new drugs for UC treatment with definite efficacy, few side effects and appropriate prices has become an urgent clinical need. With the development of traditional Chinese medicine and ethnic medicine in my country, Tibetan medicine has shown great advantages in the prevention and treatment of chronic diseases, especially in the treatment of UC. Summary of the invention

[0004] The present invention develops a formula that can treat ulcerative colitis. The ethanol extract and the drug based on the formula can achieve the purpose of treating ulcerative colitis by restoring the intestinal length of the body, protecting the integrity of the intestinal barrier of the body, regulating the imbalance of the intestinal acid-base homeostasis of the body, inhibiting the death of intestinal epithelial cells of the body and reducing the level of ROS in the intestinal tract of the body.

[0005] In order to achieve the above object, the present invention can adopt the following technical solutions:

[0006] On one hand, the present invention provides a prescription for treating ulcerative colitis, which includes Rhododendron anthopogonoides Maxim, Elettaria cardamomum (L.) Maton and Alpinia officinarum Hance. It should be noted that according to Tibetan medicine theory, the three-flavor Rhododendron anthopogonoides Maxim prescription not only has the functions of soothing the liver and invigorating the stomach and strengthening the body and strengthening the heart, but also has a good effect in treating ulcerative colitis. It has the functions of soothing the liver and invigorating the stomach and strengthening the body and strengthening the heart. In addition, the prescription is simple and 2 of the 3 medicinal materials are medicinal materials of the same origin as food, and the drugability is very good.

[0007] Furthermore, the weight ratio of the above-mentioned Rhododendron australis, cardamom and galangal is 2:1:1, 1:1:1 or 20:15:8.

[0008] It should be noted that the weight ratio of Rhododendron odoriferum, Cardamom and Alpinia officinalis can be adjusted according to specific circumstances. In the present invention, the preferred weight ratio is 2:1:1. The alcohol extract at this weight ratio has a better therapeutic effect than other ratios.

[0009] Another aspect of the present invention provides a formula extract for treating ulcerative colitis, which is obtained by extracting the above formula using ethanol or water.

[0010] It should be noted that the above-mentioned formula can be extracted with ethanol and then used as the medicine, and the extraction method can be carried out according to methods known in the art.

[0011] Furthermore, the preparation method of the above-mentioned formula extract includes one of the following methods: (1) crushing rhododendron odoriferum, cardamom and galangal, using ethanol hot reflux extraction, centrifuging, rotary evaporation and freeze-drying to obtain the formula extract; wherein the weight ratio of rhododendron odoriferum, cardamom and galangal is 2:1:1 (2) crushing rhododendron odoriferum, cardamom and galangal, using hydrothermal reflux extraction, centrifuging, rotary evaporation and freeze-drying to obtain the formula extract; wherein the weight ratio of rhododendron odoriferum, cardamom and galangal is 2:1:1, 1:1:1 or 20:15:8.

[0012] It should be noted that the ethanol extraction formula as described above can be extracted using methods known in the art, and the above method is preferred in the present invention. The extract extracted according to the above method has better therapeutic effect than other methods. More preferably, the above preparation method may include: crushing Rhododendron australis, cardamom and galangal into 80 mesh, extracting twice with water or 80% ethanol by hot reflux, combining the filtrate, centrifuging, rotary evaporating and freeze-drying to obtain the formula extract.

[0013] In another aspect, the present invention provides a medicine for treating ulcerative colitis, which comprises the above-mentioned formula extract.

[0014] It should be noted that the extract of the above-mentioned formula can be prepared into a medicine for treating ulcerative colitis. Of course, the extract of the formula alone can also be used directly.

[0015] Furthermore, the above-mentioned medicine may also include other effective active ingredients for treating or improving ulcerative colitis.

[0016] It should be noted that, in order to increase the efficacy, the extract of the formula of the present invention can be combined with other effective active ingredients that can treat or improve ulcerative colitis to prepare a medicine for treating ulcerative colitis. It should be understood that other effective active ingredients that can treat or improve ulcerative colitis are well known in the art, such as 5-aminosalicylic acid drugs, rhubarb, aloe, senna and other traditional Chinese medicines or traditional Chinese medicine polyphenols, alkaloids, quinones, terpenes and other active ingredients.

[0017] Furthermore, the above-mentioned medicine may also include a pharmaceutically acceptable carrier.

[0018] It should be noted that, in order to prepare the above-mentioned drugs into different dosage forms, pharmaceutically acceptable carriers can be added for preparation, and the pharmaceutically acceptable carriers are suitable for powders, granules, tablets, pills, capsules, oral liquids or ointments. In addition, those skilled in the art can select pharmaceutically acceptable carriers according to the specific dosage form.

[0019] In another aspect, the present invention provides a use of the above-mentioned formula for treating ulcerative colitis or the extract of the above-mentioned formula in the preparation of a medicine for treating ulcerative colitis.

[0020] Furthermore, the above-mentioned applications include one or more of the following applications: (1) application of a formula or a formula extract for treating ulcerative colitis in the preparation of a drug for restoring the length of the intestine of the body; (2) application of a formula or a formula extract for treating ulcerative colitis in the preparation of a drug for protecting the integrity of the intestinal barrier of the body; (3) application of a formula or a formula extract for treating ulcerative colitis in the preparation of a drug for regulating the imbalance of the intestinal acid-base homeostasis of the body; (4) application of a formula or a formula extract for treating ulcerative colitis in the preparation of a drug for inhibiting the death of intestinal epithelial cells of the body; (5) application of a formula or a formula extract for treating ulcerative colitis in the preparation of a drug for reducing the level of ROS in the intestine of the body.

[0021] Furthermore, the dosage form of the drug in the above application may include powder, granules, tablets, pills, capsules, oral liquid or ointment.

[0022] The beneficial effects of the present invention include at least: the prescription, prescription extract and medicine for treating ulcerative colitis provided by the present invention can effectively restore the length of the body's intestine, protect the integrity of the body's intestinal barrier, regulate the imbalance of the body's intestinal acid-base homeostasis, inhibit the body's intestinal epithelial cell death and reduce the body's intestinal ROS level, thereby achieving the purpose of treating ulcerative colitis. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1Effects of the alcohol extract of Tibetan medicine Sanwei Dujuan Fang (2:1:1) on the viability of RLE-6TN cells, n=6; compared with the normal control group, **P<0.01, *P<0.05;

[0024] Figure 2 The effect of the water extract of Sanwei Dujuan Fang (1:1:1) on the viability of RLE-6TN cells, n = 6; compared with the normal control group, **P < 0.01, *P < 0.05;

[0025] Figure 3 The effect of the Tibetan medicine Sanwei Dujuan Fang water extract (2:1:1) on the viability of RLE-6TN cells, n = 6; compared with the normal control group, **P < 0.01, *P < 0.05;

[0026] Figure 4 The effect of the Tibetan medicine Sanwei Dujuan Fang water extract (20:15:8) on the viability of RLE-6TN cells, n = 6; compared with the normal control group, **P < 0.01, *P < 0.05;

[0027] Figure 5 is the survival rate of female fruit flies in each group (Percent survival-Female);

[0028] Figure 6 is the survival rate of male fruit flies in each group (Percent survival-Mmale);

[0029] Figure 7 The motor ability of each group of fruit flies;

[0030] Figure 8 The excretion capacity of each group of fruit flies;

[0031] Fig. 9 To use the real fruit flies after the "Smurf" experiment;

[0032] Fig.10 The percentage of "Smurf" fruit flies obtained for the "Smurf" experiment;

[0033] Fig.11 The intestinal length of Drosophila after 72 hours of DSS modeling at different concentrations;

[0034] Fig.12 Different intestinal acid-base homeostasis imbalances in UC flies;

[0035] Fig.13 The proportion of UC fruit flies with different intestinal acid-base homeostasis imbalances regulated by different concentrations of the Tibetan medicine Sanwei Dujuan Fang alcohol extract;

[0036] Fig.14 The improvement of intestinal cells by the alcohol extract of Tibetan medicine Sanwei Dujuan Fang marked with trypan blue dye;

[0037] Fig.15 The improvement of intestinal cells by different concentrations of the Tibetan medicine Sanwei Dujuan Fang alcohol extract;

[0038] Fig.16 This is the fluorescently labeled fly intestinal epithelial cells after administration of the Tibetan medicine Sanwei Dujuan Fang alcohol extract;

[0039] Fig.17 is the fluorescence intensity of different groups of experiments;

[0040] Fig.18 The fluorescence of dihydroethidium (DHE) dye labeling in different groups of experiments;

[0041] Fig.19 The results of different experimental groups on the oxidative damage of the intestine of UC fruit flies. DETAILED DESCRIPTION

[0042] The examples are provided to better illustrate the present invention, but the present invention is not limited to the examples. Therefore, those skilled in the art may make non-essential improvements and adjustments to the implementation scheme according to the above invention content, which still fall within the protection scope of the present invention.

[0043] The terms used herein are only used to describe specific embodiments and are not intended to limit the present disclosure. Unless the context has a significantly different meaning, expressions in the singular include expressions in the plural. As used herein, it should be understood that terms such as "include", "have", "include" are intended to indicate the presence of features, numbers, operations, components, parts, elements, materials or combinations. The terms of the present invention are disclosed in the specification, and are not intended to exclude the possibility that one or more other features, numbers, operations, components, parts, elements, materials or combinations thereof may exist or may be added. As used herein, " / " may be interpreted as "and" or "or", depending on the circumstances.

[0044] In order to better understand the present invention, the content of the present invention is further explained below in conjunction with specific examples, but the content of the present invention is not limited to the following examples.

[0045] 1. Preparation of Tibetan medicine three-flavor azalea prescription extract

[0046] Example 1 Preparation of 2:1:1 alcohol extract of Tibetan medicine Sanwei Dujuan prescription

[0047] Rhododendron australis, cardamom and galangal were mixed in a mass ratio of 2:1:1 and crushed into 80 mesh, extracted twice with 80% ethanol under hot reflux, filtered at 0.45 μm, the filtrates were combined, centrifuged at 5000 rad / min, the supernatant was evaporated under reduced pressure at 45°C and -0.075 MPa, the concentrate was stored at -80°C for 72 hours, and freeze-dried at -86.8°C and 8 Pa for 60 hours to obtain the Tibetan medicine three-flavor rhododendron recipe ethanol extract (2:1:1).

[0048] Example 2 Preparation of 1:1:1 water extract of Tibetan medicine Sanwei Dujuan Fang

[0049] Rhododendron australis, cardamom and galangal were mixed in a mass ratio of 1:1:1 and crushed into 80 mesh size. The mixture was extracted with distilled water under hot reflux twice, filtered at 0.45 μm, the filtrates were combined, centrifuged at 5000 rad / min, the supernatant was evaporated at 60°C and -0.075 MPa under reduced pressure, the concentrate was stored at -80°C for 72 hours, and freeze-dried at -86.8°C and 8 Pa for 60 hours to obtain the Tibetan medicine three-flavor rhododendron recipe water extract (1:1:1).

[0050] Example 3 Preparation of 2:1:1 water extract of Tibetan medicine Sanwei Dujuan Fang

[0051] Rhododendron australis, cardamom and galangal were mixed in a mass ratio of 2:1:1 and crushed into 80 mesh. The mixture was extracted with distilled water under hot reflux twice, filtered at 0.45 μm, the filtrates were combined, centrifuged at 5000 rad / min, the supernatant was evaporated at 60°C and -0.075 MPa under reduced pressure, the concentrate was stored at -80°C for 72 hours, and freeze-dried at -86.8°C and 87 Pa for 60 hours to obtain the Tibetan medicine three-flavor rhododendron recipe water extract (2:1:1).

[0052] Example 4 Preparation of Tibetan medicine three-flavor azalea prescription 20:15:8 water extract

[0053] Rhododendron australis, cardamom and galangal were mixed in a mass ratio of 20:15:8 and crushed into 80 mesh, extracted twice with distilled water, filtered at 0.45 μm, the filtrate was combined, centrifuged at 5000 rad / min, the supernatant was evaporated at 60°C, -0.075 MPa, the concentrate was stored at -80°C for 72 h, and freeze-dried at -86.8°C, 8 Pa for 60 h to obtain the Tibetan medicine three-flavor rhododendron formula water extract (20:15:8). 2. Toxicity experiment of drug-containing intestinal absorption solution with different prescription ratios on RLE-6TN cells

[0054] The above-prepared Tibetan medicine Sanwei Dujuan prescription alcohol extract (2:1:1), Sanwei Dujuan prescription water extract (1:1:1), Tibetan medicine Sanwei Dujuan prescription water extract (2:1:1) and Tibetan medicine Sanwei Dujuan prescription water extract (20:15:8) were respectively prepared into solutions with concentrations of 0.032 mg / ml, 0.16 mg / ml, 0.8 mg / ml and 4 mg / ml. The extracts of different concentrations and different prescription ratios were added to RLE-6TN cells and cultured for 24 hours. The RLE-6TN cell viability was determined using a CCK-8 kit. Each extract and each concentration were repeated 6 times to evaluate the effects of extracts of different prescription ratios on the viability of RLE-6TN cells. The results are as follows Figures 1 to 4 As shown, the results showed that each ratio of the formula and its different extracts had no obvious toxicity to cells at a concentration of 0.8 mg / ml, among which the ethanol extract of the Tibetan medicine Sanwei Rhododendron formula (2:1:1) had the most ideal toxicity.

[0055] 3. Comparative study on the efficacy of different proportions of Sanwei Dujuan prescription intestinal absorption liquid against ulcerative colitis

[0056] The median effective dose (EC50) of the above-prepared Tibetan medicine Sanwei Dujuan prescription ethanol extract (2:1:1), Sanwei Dujuan prescription water extract (1:1:1), Tibetan medicine Sanwei Dujuan prescription water extract (2:1:1) and Tibetan medicine Sanwei Dujuan prescription water extract (20:15:8) was tested respectively. 50 ) found that the intestinal absorption liquid containing Sanwei Dujuan prescription with different ratios had good anti-ulcerative colitis effect, among which the EC of the alcohol extract of Sanwei Dujuan prescription (2:1:1) 50 The maximum indicates that it has the best safety and effectiveness. The results are shown in Table 1 below.

[0057] Table 1 The median effective dose of different formulas and extracts

[0058] Three-flavor azalea medicine-containing intestinal absorption liquid EC50(mg / ml) 1:1:1 Three-flavor Rhododendron water extract 0.4232 20:15:8 Three kinds of rhododendron water extract 0.5196 2:1:1 Three-flavor Rhododendron water extract 0.4065 2:1:1 Three-flavor Rhododendron alcohol extract 0.5385

[0059] 4. The Tibetan medicine Sanwei Dujuan prescription alcohol extract has a protective effect on the physiological function damage caused by Drosophila ulcerative colitis (UC) model

[0060] In the present embodiment, in order to clarify the anti-inflammatory effect of the alcohol extract of Tibetan medicine Sanwei Dujuan Fang (2:1:1) (hereinafter referred to as the alcohol extract of Tibetan medicine Sanwei Dujuan Fang), the survival rate of male and female fruit flies under the stimulation of dextran sulfate sodium salt (DSS) was measured. 1118 Drosophila strains, 20 per bottle, starved for 30 min, and then the flies in different treatment groups were moved to filter paper culture medium dripped with 4% DSS (5% sucrose). The number of dead flies was recorded every 6 or 12 h, and the filter paper was replaced every 72 h. Each group was repeated for 9 bottles in total. The results are shown in Figure 5 and Figure 6 The results showed that the ethanol extract of Tibetan medicine Sanwei Dujuan Fang significantly prolonged the survival rate of DSS-induced UC fruit flies, indicating that the ethanol extract of Tibetan medicine Sanwei Dujuan Fang has a significant anti-inflammatory effect. Figure 5 and Figure 6 As shown, the results showed that the ethanol extract of Tibetan medicine Sanwei Dujuan Fang significantly prolonged the survival rate of DSS-induced UC fruit flies, indicating that the ethanol extract of Tibetan medicine Sanwei Dujuan Fang has a significant anti-inflammatory effect.

[0061] In the embodiment of the present invention, the locomotor ability and excretion ability of fruit flies are further determined to clarify the effect of the alcohol extract of the Tibetan medicine Sanwei Dujuan Fang on the overall physiological function of DSS-induced UC fruit flies. The locomotor ability determination operation is as follows: 20 fruit flies that have emerged for 3 days in ordinary culture medium and drug-containing culture medium are randomly collected, and fed with a solution with or without 4% DSS (5% sucrose) for 24 hours. Gently shake the fruit flies to the bottom of the test tube and plug the bottle cap. Record the number of fruit flies that climbed more than 8 cm in height within 5 seconds. The experiment was repeated 5 times in an environment of 25°C and 60% humidity. The excretion ability determination operation is as follows: Fruit flies that have emerged for 2-4 days in ordinary culture medium and drug-containing culture medium are randomly collected in agar culture medium and starved for 6 hours, 20 in each group, and then the fruit flies are placed in 2ml centrifuge tubes containing 5% sucrose + 2% bromophenol blue reagent 200ul, and fed for 12 hours. After feeding, place the 2 ml centrifuge tube in a 5 ml centrifuge tube and shake, take 150 ul into a 96-well plate, and measure the OD 625 The analysis results are as follows Figure 7 and Figure 8 As shown in the results, compared with the model group, the Tibetan medicine Sanwei Dujuan Fang ethanol extract can improve the movement ability and excretion volume of UC fruit flies after administration. Therefore, the Tibetan medicine Sanwei Dujuan Fang ethanol extract has a protective effect on the physiological function damage caused by the Drosophila UC model.

[0062] 5. The Tibetan medicine Sanwei Dujuan prescription alcohol extract has an improving effect on the intestinal morphology and acid-base disorder of UC fruit flies

[0063] Randomly collect w 2-4 days after eclosion 1118 The female fruit flies of the strain were fed in ordinary culture medium and drug-containing culture medium for 7 days, and the model was established with 4% DSS for 72 hours. The fruit flies of the corresponding groups were collected and starved in agar culture medium for 6 hours; (1) The above fruit flies were placed in a fruit fly tube containing 5% sucrose + 2% bromophenol blue reagent and fed for 18 hours; the number of "Blue Smurfs" was counted after selection under a microscope. (2) 15-20 complete intestines of the above fruit flies were separated under a microscope and placed in PBS. The changes in intestinal morphology were observed under a microscope and the length of the intestine was measured with a vernier caliper. (3) The above fruit flies were placed in a fruit fly tube containing 5% sucrose + 4% pH reagent and fed for 4 hours. 15-20 complete fruit fly intestines were separated under a microscope and the copper cell area was observed. The above experiments were repeated 3 times for each group.

[0064] The integrity of the intestinal barrier was evaluated by the "Smurf" experiment. The results showed that after 72 hours of 4% DSS modeling, the fruit flies showed obvious "Smurf" phenomenon, indicating that the intestinal barrier was damaged. The alcohol extract of the Tibetan medicine Sanwei Dujuan Fang played a protective role on the intestinal barrier of the fruit flies ( Fig. 9 and Fig.10 ). After 72 hours of modeling with 4% DSS, the intestinal length of Drosophila was significantly shortened. After administration of the Tibetan medicine Sanwei Dujuan Fang alcohol extract, the intestinal length of Drosophila was significantly restored ( Fig.11 ).

[0065] In addition, the intestinal pH test of Drosophila showed that the alcohol extract of Sanwei Dujuan Fang, a Tibetan medicine, could regulate the imbalance of intestinal acid-base homeostasis in UC Drosophila ( Fig.12 and Fig.13 ).

[0066] In summary, the ethanol extract of Tibetan medicine Sanwei Dujuan Fang can restore the intestinal length of Drosophila, protect the integrity of the intestinal barrier, and regulate acid-base homeostasis imbalance.

[0067] 6. Tibetan medicine Sanwei Dujuan prescription alcohol extract can inhibit the death of Drosophila intestinal epithelial cells

[0068] When UC occurs, programmed cell death of intestinal epithelial cells increases, thereby destroying the intestinal barrier. Fruit flies that were 2-4 days old at eclosion were randomly collected, and after 2 days of recovery, they were starved for 2 hours and modeled with 4% DSS for 72 hours. The fruit flies were then transferred to 0.4% trypan blue food and fed for 4 hours. 15-20 complete fruit fly intestines were separated under a microscope and observed under a microscope. Each group of experiments was repeated 3 times. Trypan blue dye was used to mark dead intestinal cells. After 72 hours of 4% DSS modeling, the blue degree of the fruit fly midgut increased significantly, indicating that DSS would cause intestinal epithelial cell death. After administration of the Tibetan medicine Sanwei Dujuan Fang alcohol extract, the degree of intestinal epithelial cell death was improved ( Fig.14 and Fig.15 ).

[0069] To further verify the death of intestinal epithelial cells in Drosophila, Myo1A-Gal4;UAS-GFP strain Drosophila that had emerged for 2-4 days were randomly collected. After 2 days of recovery, they were starved for 2 hours and modeled with 4% DSS for 72 hours. 15-20 complete Drosophila intestines were separated under a microscope and placed in 4% paraformaldehyde, fixed at room temperature for 30 minutes, washed with PBS 3 times, 5 minutes each time, stained with DAPI for 10 minutes, washed with PBS 3 times, 5 minutes each time, and observed and photographed under a fluorescence microscope after glycerol sealing. Each group of experiments was repeated 3 times. Using Myo1A-Gal4;UAS-GFP transgenic Drosophila that marked intestinal epithelial cells, 4% DSS modeling was performed for 72 hours. After the administration of Tibetan medicine Sanwei Dujuan Fang ethanol extract, the fluorescence intensity in the intestine was significantly enhanced compared with the model group, indicating that the Tibetan medicine Sanwei Dujuan Fang ethanol extract can inhibit the death of Drosophila intestinal epithelial cells ( Fig.16 and Fig.17 ).

[0070] VII. The alcohol extract of Tibetan medicine Sanwei Dujuan Fang can improve the intestinal oxidative damage test of Drosophila

[0071] UC is usually associated with excessive production of ROS. The peroxide indicator dihydroethidium (DHE) dye can be used to measure the content of ROS in the intestine. The specific operation is as follows: randomly collect fruit flies that have ecloded for 2-4 days in ordinary culture medium, starve for 2 hours after recovery for 2 days, and model with 4% DSS for 72 hours. Separate 15-20 complete fruit fly intestines under a microscope, place them in PBS, add 1:200 diluted DHE to stain at room temperature for 30 minutes, and wash 3 times with PBS for 5 minutes each time. Fix with 4% formaldehyde for 30 minutes, wash 3 times with PBS for 5 minutes each time, stain with DAPI for 10 minutes, and wash 3 times with PBS for 5 minutes each time. Observe under a fluorescence microscope after sealing with glycerol, and repeat each group of experiments 3 times.

[0072] The results are as follows Fig.18 and Fig.19 As shown, the results showed that after 48 hours of 4% DSS modeling, the intensity of DHE in the intestine increased significantly. After administering the Tibetan medicine Sanwei Dujuan Fang ethanol extract, the intensity of DHE in the intestine decreased, indicating that the Tibetan medicine Sanwei Dujuan Fang ethanol extract can reduce the ROS level in the fruit fly intestine.

[0073] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the purpose and scope of the technical solution of the present invention, which should be covered by the scope of the claims of the present invention.

Claims

1. Use of a composition extract in the preparation of a drug for treating ulcerative colitis, wherein the composition comprises Rhododendron australis, Elaeagnus cardamomum and Alpinia officinalis in a mass ratio of 2:1:1, and the composition extract is obtained by ethanol extraction.

2. The use according to claim 1, characterized in that: Applications include one or more of the following: (1) Use of the extract of the combination in the preparation of a drug for restoring the length of the intestine of the body; (2) Use of the extract of the combination in the preparation of a drug for protecting the integrity of the intestinal barrier of the body; (3) Use of the extract of the combination in the preparation of a drug for regulating the imbalance of the acid-base homeostasis of the body's intestine; (4) Use of the extract of the combination in the preparation of a drug for inhibiting the death of intestinal epithelial cells of the body; (5) Use of the extract of the combination in the preparation of a drug for reducing the level of ROS in the intestine of the body.

3. The use according to claim 1 or 2, characterized in that: The preparation method of the composite extract comprises one of the following methods: (1) crushing Rhododendron australis, cardamom and Alpinia galanga, extracting with ethanol hot reflux, centrifuging, rotary evaporating and freeze-drying to obtain the composite extract.

4. The use according to claim 3, characterized in that: The preparation method of the composite extract comprises: crushing rhododendron australis, cardamom and galangal into 80 meshes, extracting twice with water or 80% ethanol by hot reflux, combining filtrates, centrifuging, rotary evaporating and freeze-drying to obtain the composite extract.

5. The use according to any one of claims 1, 2 or 4, characterized in that: The medicament also includes a pharmaceutically acceptable carrier.

6. The use according to claim 5, characterized in that: The pharmaceutically acceptable carrier is suitable for powders, granules, tablets, pills, capsules, oral solutions or ointments.

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