Cell nutrient for resisting aging and prolonging life of middle-aged and elderly pets as well as preparation method and application of cell nutrient

Abstract

The invention discloses an anti-aging and life-prolonging cell nutrient for middle-aged and elderly pets as well as a preparation method and application thereof, and relates to the technical field of pet food. The anti-aging and life-prolonging cell nutrient for the middle-aged and old pets comprises the following components in parts by weight: 1-10 parts of an edible mushroom ergothioneine extract, 1-10 parts of krill oil, 0.5-5 parts of collagen peptide and 0.5-5 parts of an antioxidant active component. The antioxidant active ingredients in the cell nutrient can form a synergistic effect with the edible mushroom ergothioneine extract, and the anti-aging effect can be enhanced; the ergothioneine and the krill oil are short in metabolism and transport path in vivo and can directly reach damaged cells of pet dogs and cats; the cell nutrient disclosed by the invention can improve skin barrier, remove cell free radicals, protect mitochondria, improve inflammation, improve immunity and prolong life of pets from inside to outside.

Description

Technical Field

[0001] The invention relates to the technical field of pet food, and in particular to a cell nutrient for resisting aging and prolonging life of middle-aged and elderly pets, and a preparation method and application thereof. Background Art

[0002] The rise of the pet-keeping craze in China was concentrated in 2015-2017. By 2023, the number of middle-aged and elderly pet dogs and cats in my country will reach 6 million and 1.4 million respectively, with middle-aged and elderly pets accounting for 6%-8%. In the future, the number of pets entering the middle-aged and elderly stage (>8 years old) will grow explosively, and it is estimated that it will reach 13 million+ in 2024. Pet health foods for anti-aging and longevity will receive more attention.

[0003] The free radical theory holds that aging is caused by damage to cellular components caused by excessive free radicals. When pets enter old age, the body's ability to purify free radicals begins to decline, and free radicals begin to accumulate excessively, causing cytotoxicity, phospholipid damage, telomere shortening, inflammation, and mitochondrial dysfunction, which ultimately leads to aging and death. All organs, from bones, internal organs, brain to skin, hair, and eyes, are affected, the body's immunity declines, and diseases such as the heart and urinary system enter an outbreak period.

[0004] In order to fight against the damage of these free radicals, our body has a complex antioxidant defense system. Antioxidant effect can be defined as the ability to remove the remaining reactive oxygen species or free radicals produced in the body, or to inhibit the adverse effects of these abilities on the organism. At present, the anti-aging effect is usually achieved by improving the body's antioxidant function.

[0005] Patent CN 118236304A discloses an anti-aging preparation of cat's claw bean ethanol extract and its preparation method and application. The cat's claw bean ethanol extract is used as the main effective ingredient, and the synergistic effect between the cat's claw bean ethanol extract and NMN (β-nicotinamide mononucleotide) is utilized. The anti-aging effect of the cat's claw bean ethanol extract and NMN is maximized by adjusting the addition ratio of the two. The cat's claw bean of the invention itself also has alkaloids, which can easily cause pet poisoning if not handled properly, and the safety of long-term consumption cannot be guaranteed.

[0006] Patent CN 116440162A provides a biological product with anti-aging effect on the cardiac system of mammals and its preparation method. The biological product contains exosomes and miRNA extracted from blood, and the source is simple and extensive, which is conducive to long-term, repeated and large-scale use. The invention is a biological product, mainly for intravenous injection, and its cost is too high for pets.

[0007] Patent CN 117204517 A discloses a high-protein cat food and its preparation method. The cat food can improve the resistance of elderly cats and play a role in delaying the aging of elderly cats. This method starts from the method of increasing protein to increase the nutritional value of elderly pets, and at the same time supplements vitamin E, spore oil and rosemary extract to enhance the antioxidant effect. This invention only supplements nutrition such as increasing protein, and the antioxidant used has poor stability and is easily oxidized. Summary of the invention

[0008] In order to solve the problems existing in the above-mentioned prior art, the purpose of the present invention is to provide a cellular nutrient for anti-aging and longevity of middle-aged and elderly pets, and a preparation method and application thereof. The cellular nutrient for anti-aging and longevity of middle-aged and elderly pets of the present invention has superior performance compared with commonly used antioxidants, is easy to obtain, and has good stability and high safety. It has high application value in anti-aging and longevity of middle-aged and elderly pets, and can be directly added in the batching stage.

[0009] The present invention provides the following technical solutions:

[0010] The invention provides a cell nutrient for middle-aged and elderly pets to resist aging and prolong life. The cell nutrient comprises the following components in parts by weight: 1-10 parts of edible fungus ergothioneine extract, 1-10 parts of krill oil, 0.5-5 parts of collagen peptides and 0.5-5 parts of antioxidant active ingredients.

[0011] Further, the preparation method of the edible fungus thioneine extract comprises the following steps:

[0012] (1) Vacuum freeze drying: freeze-dry fresh edible fungi at a temperature below -50°C and a vacuum degree below 20 Pa, and crush and screen to obtain edible fungi powder;

[0013] (2) Extraction and purification of thioneine: edible fungus powder and ethanol solution with a mass concentration of 40-70% are mixed in a mass ratio of 1: (20-30), and extracted in a constant temperature water bath at a water temperature of 45-65° C. for 20-40 min; then microwaved at 60-70° C. for 3-8 min; then ultrasonically extracted for 3-8 min to obtain an extract; the extract is centrifuged at room temperature, the supernatant is collected and purified and filtered to obtain an edible fungus thioneine purified liquid;

[0014] (3) Drying of thioneine: The purified thioneine solution of edible fungi is concentrated under negative pressure to a wall-hanging state, and then transferred to a vacuum freeze drying box for further freeze drying to obtain crystals, and then the crystals are ground to obtain the thioneine extract of edible fungi.

[0015] Furthermore, in the preparation method of the edible fungus ergothioneine extract, step (1) also includes first slicing the edible fungus and cooling it to below -10°C, taking it out and quickly placing it flat on a material rack of a pre-cooled vacuum freeze dryer, and then vacuum freeze-drying it.

[0016] Furthermore, in the preparation method of the edible fungus thioneine extract, the purification and filtration step is specifically to first filter with a 300-mesh filter cloth, and then purify and filter using a polytetrafluoroethylene microporous filter membrane with a pore size of 0.45 μm.

[0017] Furthermore, the edible fungus is selected from at least one of boletus, pleurotus eryngii, king oyster mushroom, enoki mushroom, oyster mushroom, shiitake mushroom, Agaricus blazei, Hericium erinaceus, Agrocybe bisporus and so on.

[0018] Further, described thioneine is the only natural antioxidant that can directly enter mitochondria and nucleus, can protect mitochondria from being damaged by oxidation while directly removing reactive oxygen free radicals, and thioneine can be enriched in body tissues and cells, can be accurately transported to required damaged cells and tissues by transporter.Thioneine can also react with divalent metal cations such as iron and copper to produce chelation, thereby reducing or preventing the ability of these metal ions to stimulate oxidative damage.In addition, because thioneine has special thionone structure and higher redox potential, it is not prone to self-oxidation under physiological pH conditions, more stable compared with other thiol antioxidants (such as: glutathione), better free radical scavenging activity than other common antioxidants such as glutathione, vitamin E analogs, and has very strong thermal stability.

[0019] Furthermore, the polypeptide molecular weight of the collagen peptide is less than 3000Da.

[0020] Furthermore, the collagen peptide is selected from one of ray collagen peptide, type II collagen peptide, avian cartilage collagen peptide, shark cartilage collagen peptide, and deep-sea fish collagen peptide.

[0021] Furthermore, the antioxidant active ingredient is selected from at least one of resveratrol, coenzyme Q10, glutathione, vitamin C, anthocyanin, astaxanthin, quercetin, lutein, rosemary extract, and grapefruit extract.

[0022] Furthermore, the total phospholipid content of the krill oil is ≥40%.

[0023] Furthermore, compared with fish oil, the omega-3 in krill oil is almost entirely in the form of phospholipids (phosphatidylcholine), which is emulsifiable and easily absorbed directly into the cell membrane, making it more conducive to digestion and absorption. Secondly, the phospholipid omega-3 in krill oil is directly transported to the liver through the portal vein and rapidly distributed to all cells in the body. In addition, krill oil also contains choline and astaxanthin. Choline is an essential nutrient that is crucial for nerve signaling and helps the liver, brain and muscle function; astaxanthin is a natural antioxidant that gives krill oil a ruby-like bright color and protects the product from oxidation without the need to add preservatives. The omega-3 in fish oil is in the form of triglycerides and cannot enter the liver directly, but must take a longer route through the lymphatic system.

[0024] Secondly, the present invention also provides a method for preparing the above-mentioned cell nutrients for anti-aging and life extension of middle-aged and elderly pets, comprising the following steps:

[0025] S1: stirring and mixing the edible fungus thioneine extract, collagen peptide, and antioxidant active ingredients in proportion to make them evenly mixed to obtain a mixed powder;

[0026] S2: Add the mixed powder into warm water at 40-60°C while stirring to fully dissolve it to obtain aqueous phase A; the mass ratio of the mixed powder to warm water is 1:(3-4);

[0027] S3: mixing the aqueous phase A and the krill oil, and obtaining an emulsion after high-speed shear emulsification;

[0028] S4: The obtained emulsion is subjected to high-pressure homogenization treatment to obtain cell nutrients for anti-aging and life-extending of middle-aged and elderly pets.

[0029] In addition, the present invention also provides an application of the above-mentioned cell nutrients for anti-aging and life-extending of middle-aged and elderly pets in pet food.

[0030] Finally, the present invention also provides a pet food prepared by using the above-mentioned cellular nutrients for anti-aging and prolonging life of middle-aged and elderly pets, wherein the added amount of the cellular nutrients for anti-aging and prolonging life of middle-aged and elderly pets is 0.5-5% of the mass of the pet food.

[0031] Furthermore, the pet food is selected from one of pet staple food, nutritional supplements, and pet snacks.

[0032] The present invention has the following technical effects:

[0033] The antioxidant active ingredients in the cell nutrients of the present invention can form a synergistic effect with the ergothioneine extract of edible fungi, and can enhance the anti-aging effect; ergothioneine and krill oil have short metabolism and transport pathways in the body and can directly reach the damaged cells of pet dogs and cats; the cell nutrients of the present invention can enhance the skin barrier from the inside out, remove cell free radicals, protect mitochondria, improve inflammation, enhance immunity, and prolong the life of pets. DETAILED DESCRIPTION

[0034] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] It should be understood that when used in this specification and the appended claims, the terms "include" and "comprises" indicate the presence of described features, integers, steps, operations, elements and / or components, but do not exclude the presence or addition of one or more other features, integers, steps, operations, elements, components and / or combinations thereof.

[0036] In order to more fully understand the technical content of the present invention, the technical solution of the present invention is further introduced and illustrated in conjunction with specific embodiments below.

[0037] Example 1

[0038] A cell nutrient for middle-aged and elderly pets for anti-aging and life extension comprises the following components in parts by weight: 7 parts of boletus ergothioneine extract, 8 parts of krill oil (total phospholipid content = 41%), 2 parts of ray collagen peptide (polypeptide molecular weight = 2000±1000Da), and 1 part of antioxidant active ingredient (rosemary extract: natural vitamin E = 2:1).

[0039] The preparation method of the boletus ergothioneine extract comprises the following steps:

[0040] (1) Vacuum freeze drying: Wash the fresh porcini, slice them, and place them in a quick freezer to quickly cool them to -20°C. Take them out and quickly place them flat on the material rack of the pre-cooled vacuum freeze dryer. Freeze dry them for 72 hours at a cold trap temperature of -30°C and a vacuum degree of 20Pa in the vacuum freeze dryer. Take out the dried porcini, place them in a high-speed grinder, fully grind them through a 70-mesh sieve, seal them, and place them in a -20°C refrigerator for later use.

[0041] (2) thioneine extraction and purification: the edible fungus powder prepared is mixed with 62% ethanol in a feed-liquid mass ratio of 1:25, extracted in 55 DEG C of constant temperature water baths for 30min, then placed in a microwave instrument at a power of 400W, and processed for 5min at a temperature of 70 DEG C, and finally extracted using an ultrasonic cell crusher at an ultrasonic power of 440W and an ultrasonic time of 5min to obtain an extract. The extract is centrifuged at room temperature (rotating speed is 8000r / min, and the time is 10min), and supernatant is collected as thioneine extract. First filter with 300 mesh filter cloth, then purify and filter by 0.45 μm PTFE microporous filter membrane with an aperture to obtain Bolete thioneine purified liquid.

[0042] (3) Drying of thioneine: The purified bolete thioneine solution was subjected to negative pressure rotary evaporation at 50°C, concentrated to a wall-hanging state, and transferred to a vacuum freeze drying oven for further freeze drying (temperature was -30°C, vacuum degree was 8 Pa) for 48 h to obtain crystals. The crystals were ground and passed through a 100 mesh sieve to obtain a bolete thioneine extract. The thioneine content thereof was 6.8 mg / g as detected by high performance liquid chromatography.

[0043] A method for preparing cell nutrients for anti-aging and life extension of middle-aged and elderly pets, comprising the following steps:

[0044] S1: Stir and mix all the nutrients except krill oil according to a certain proportion to make them evenly mixed to obtain a mixed powder.

[0045] S2: Add the mixed powder to 50°C warm water while stirring to dissolve it, so that it is fully dispersed and dissolved to obtain a water phase; the mass ratio of the mixed powder to warm water is 1:4.

[0046] S3: Colostrum is obtained by mixing the water phase and krill oil in a ratio of 1:1 and emulsifying them by high-speed shearing in a shearing machine.

[0047] S4: subjecting the obtained colostrum to high pressure homogenization to obtain emulsified cell nutrients.

[0048] Example 2

[0049] Different from Example 1, the raw material of the selected edible fungus thioneine extract is Agaricus bisporus, and the thioneine content of the dried thioneine powder is 0.41 mg / g by high performance liquid phase detection.

[0050] Example 3

[0051] Different from Example 1, the weight proportion of the selected edible fungus thioneine extract is 1 part.

[0052] Embodiment 4: The difference from Embodiment 1 is that the weight proportion of the selected edible fungus thioneine extract is 10 parts.

[0053] Example 5

[0054] The difference from Example 3 is that the weight proportion of the selected krill oil (total phospholipids ≥ 40%) is 10 parts.

[0055] Example 6

[0056] Different from Example 1, the selected collagen peptide is poultry collagen peptide, and the weight portion is 0.5 parts. The preparation method of the boletus ergothioneine extract and the preparation method of the cell nutrient for anti-aging and life extension of middle-aged and elderly pets are different.

[0057] The preparation method of the boletus ergothioneine extract comprises the following steps:

[0058] (1) Vacuum freeze drying: Wash the fresh porcini, slice them, and place them in a quick freezer to quickly cool them to -10°C. Take them out and quickly place them flat on the material rack of the pre-cooled vacuum freeze dryer. Freeze dry them for 72 hours at a cold trap temperature of -30°C and a vacuum degree of 20Pa in the vacuum freeze dryer. Take out the dried porcini, place them in a high-speed grinder, fully grind them through a 70-mesh sieve, seal them, and place them in a -20°C refrigerator for later use.

[0059] (2) thioneine extraction and purification: the edible fungus powder prepared is mixed with 40% ethanol in a feed-liquid mass ratio of 1:30, extracted in 65 DEG C of constant temperature water baths for 20min, then placed in a microwave instrument at a power of 400W, and processed for 8min at a temperature of 65 DEG C, and finally extracted using an ultrasonic cell crusher at an ultrasonic power of 440W and an ultrasonic time of 3min to obtain an extract. The extract is centrifuged at room temperature (rotating speed is 8000r / min, and the time is 10min), and supernatant is collected as thioneine extract. First filter with 300 mesh filter cloth, then purify and filter by 0.45 μm PTFE microporous filter membrane with an aperture to obtain Bolete thioneine purified solution.

[0060] (3) Drying of thioneine: The purified bolete thioneine solution was subjected to negative pressure rotary evaporation at 50°C, concentrated to a wall-hanging state, and transferred to a vacuum freeze drying oven for further freeze drying (temperature was -30°C, vacuum degree was 8 Pa) for 48 h to obtain crystals. The crystals were ground and passed through a 100 mesh sieve to obtain a bolete thioneine extract. The thioneine content thereof was 6.7 mg / g as detected by high performance liquid chromatography.

[0061] A method for preparing cell nutrients for anti-aging and life extension of middle-aged and elderly pets, comprising the following steps:

[0062] S1: Stir and mix all the nutrients except krill oil according to a certain proportion to make them evenly mixed to obtain a mixed powder.

[0063] S2: Add the mixed powder to 40°C warm water while stirring to dissolve it, so that it is fully dispersed and dissolved to obtain a water phase; the mass ratio of the mixed powder to warm water is 1:3.

[0064] S3: Colostrum is obtained by mixing the water phase and krill oil in a ratio of 1:1 and emulsifying them by high-speed shearing in a shearing machine.

[0065] S4: subjecting the obtained colostrum to high pressure homogenization to obtain emulsified cell nutrients.

[0066] Example 7

[0067] The difference from Example 1 is that the weight fraction of the selected antioxidant active ingredient is 0.5 parts. The preparation method of the boletus ergothioneine extract and the preparation method of the cell nutrient for anti-aging and life extension of middle-aged and elderly pets are different.

[0068] The preparation method of the boletus ergothioneine extract comprises the following steps:

[0069] (1) Vacuum freeze drying: Wash the fresh porcini, slice them, and place them in a quick freezer to quickly cool them to -20°C. Take them out and quickly place them flat on the material rack of the pre-cooled vacuum freeze dryer. Freeze dry them for 72 hours at a cold trap temperature of -30°C and a vacuum degree of 20Pa in the vacuum freeze dryer. Take out the dried porcini, place them in a high-speed grinder, fully grind them through a 70-mesh sieve, seal them, and place them in a -20°C refrigerator for later use.

[0070] (2) thioneine extraction and purification: the edible fungus powder prepared is mixed with 70% ethanol with a feed-liquid mass ratio of 120, extracted in 45 DEG C of constant temperature water baths for 30min, then placed in a microwave instrument at a power of 400W, and processed for 3min at a temperature of 60 DEG C, and finally extracted using an ultrasonic cell crusher at an ultrasonic power of 440W and an ultrasonic time of 8min to obtain an extract. The extract is centrifuged at room temperature (rotating speed is 8000r / min, and the time is 10min), and the supernatant is collected and is the thioneine extract. First filter with 300 mesh filter cloth, then filter by 0.45 μm PTFE microporous membrane purification and filtration to obtain Bolete thioneine purified liquid.

[0071] (3) Drying of thioneine: The purified bolete thioneine solution was subjected to negative pressure rotary evaporation at 50°C, concentrated to a wall-hanging state, and transferred to a vacuum freeze drying oven for further freeze drying (temperature was -30°C, vacuum degree was 8 Pa) for 48 h to obtain crystals. The crystals were ground and passed through a 100 mesh sieve to obtain a bolete thioneine extract. The thioneine content thereof was 6.71 mg / g as detected by high performance liquid chromatography.

[0072] A method for preparing cell nutrients for anti-aging and life extension of middle-aged and elderly pets, comprising the following steps:

[0073] S1: Stir and mix all the nutrients except krill oil according to a certain proportion to make them evenly mixed to obtain a mixed powder.

[0074] S2: Add the mixed powder to 60°C warm water while stirring to dissolve it, so that it is fully dispersed and dissolved to obtain a water phase; the mass ratio of the mixed powder to warm water is 1:3.5.

[0075] S3: Colostrum is obtained by mixing the water phase and krill oil in a ratio of 1:1 and emulsifying them by high-speed shearing in a shearing machine.

[0076] S4: subjecting the obtained colostrum to high pressure homogenization to obtain emulsified cell nutrients.

[0077] Example 8

[0078] Different from Example 1, the antioxidant active ingredients selected and the added ratio are astaxanthin: lutein: vitamin C = 1:4:20, and the total weight fraction is 1 part.

[0079] Example 9

[0080] The difference from Example 1 is that the weight portion of the krill oil used is 1 part; the weight portion of the collagen is 5 parts; and the weight portion of the antioxidant active ingredient is 5 parts.

[0081] Comparative Example 1

[0082] Different from Example 1, no antioxidant active ingredient was added.

[0083] Comparative Example 2

[0084] Different from Example 8, no boletus ergothioneine extract was added.

[0085] Comparative Example 3

[0086] The difference from Example 1 is that the weight fraction of the boletus ergothioneine extract is 0.9 parts.

[0087] Comparative Example 4

[0088] The difference from Example 1 is that the weight fraction of krill oil is 0.9 parts.

[0089] Comparative Example 5

[0090] Different from Example 2, the molecular weight of the ray protein peptide component is 8000±3000Da.

[0091] Comparative Example 6

[0092] Nicotinamide mononucleotide (NMN) is added to cat food, and the weight percentage of NMN in the cat food is 0.1%.

[0093] Comparative Example 7

[0094] The difference from Example 1 is that krill oil is replaced by an equal amount of fish oil (25%≤Ω3≤35%).

[0095] Comparative Example 8

[0096] Different from Example 4, no antioxidant active ingredient was added.

[0097] To further illustrate the technical effects of the present invention, the following tests were performed on the above-mentioned Examples 1-9 and Comparative Examples 1-8.

[0098] (I) Pet Testing

[0099] Test plan: 144 middle-aged and elderly dogs and cats (>6 years old, cats weighing 4±1kg, dogs weighing 10±2kg) were selected and randomly divided into 18 groups, 8 in each group, and tested individually in a quiet small room. A pre-experiment was conducted before the experiment, and the food needed to be fed one week in advance, and then the food was changed to the control food and the experimental food according to the 7-day food change schedule (day1-15%, day3-30%, day5-60%, day7-100%). Each repetition (cats were raised in a single pen in a pet cat cage, and dogs were raised in a single pen in a dog cage. They were released outdoors for free activities for 1 hour at 8:00, 13:00, and 16:00 every day). One group was fed with control food, and the rest were fed with experimental food. Immunization and deworming were carried out before the experiment. Free drinking water, change cat litter every day, clean the dog and cat house, clean up, and keep the pet pen clean. The entire test lasted 4 weeks, and the relevant indicators were measured at the end of the test.

[0100] Control group: fed with ordinary dog ​​and cat food.

[0101] Test groups 1-9: The cell nutrients prepared in Examples 1-9 were added to ordinary dog ​​and cat food, respectively, with the added amount being 1.8% of the weight of the puffed dog and cat food, and were added during the second mixing of the raw materials in the puffing process.

[0102] Test groups 10-17: Cell nutrients prepared in Comparative Examples 1-8 were added to ordinary dog ​​and cat food, respectively, with the added amount being 1.8% of the weight of the puffed dog and cat food, and were added during the second mixing of raw materials in the puffing process.

[0103] Measurement indicators (measurement frequency): food intake (daily); blood immune inflammatory response and antioxidant indicators (at the end of the experiment); skin barrier indicators (at the end of the experiment).

[0104] Among them, blood immune inflammatory response and antioxidant indicators include: superoxide dismutase (SOD) content, total antioxidant capacity (T-AOC), malondialdehyde (MDA) content, interleukin-6 (IL-6) content, tumor necrosis factor-α (TNF-α) content and blood triglyceride (TG) content.

[0105] Skin barrier indicators include: transepidermal water loss (TEWL) and skin hydration content (Sebum amount).

[0106] Among them, the test results for cats are shown in Table 1, and the test results for dogs are shown in Table 2.

[0107] Table 1 Cat effect test results

[0108]

[0109]

[0110] As can be seen from Table 1, when the cell nutrient solution in cat food does not add antioxidant active ingredients (Comparative Example 1, Comparative Example 8), its antioxidant properties are all decreased compared with Examples 1 and 4. When the cell nutrient solution in cat food does not contain edible fungus ergothioneine extract or its content is not within the scope of the present invention, its antioxidant property is greatly reduced, and the reduction effect of inflammatory factors and skin transepidermal water loss values ​​in the body is not as good as the technical scheme of the present invention. In addition, when the krill oil content of the present invention is changed, its antioxidant effect in cat body is not good, and its total antioxidant capacity is almost the same as the reference example. When the polypeptide molecular weight of collagen peptide is not within the scope of the present invention, compared with the present invention, its transepidermal water loss value is significantly improved, and the skin water content is also low. When fish oil is used to replace krill oil, the antioxidant capacity in the body is basically unchanged, but the reduction range of triglyceride levels and skin transepidermal water loss values ​​in its blood is obviously not as good as the scheme of the present invention. Comparative Example 6 uses an anti-aging ingredient NMN, and the results show that there is also a significant antioxidant effect, but the overall effect is weaker than the embodiment of the present invention, and the improvement of its barrier effect on the skin is weak when used alone.

[0111] Table 2 Dog effect test results

[0112]

[0113]

[0114] As can be seen from Table 2, the application effects of each experimental group in dogs are similar to those in cats. Further analysis combined with Table 1 shows that the antioxidant effect, blood immune inflammation reduction effect and skin barrier enhancement effect of the cell nutrition of the present invention are significantly enhanced compared with the control group, regardless of whether it is applied to cats or dogs. When the components or ratios of the present invention are changed, the effects thereof will be negatively affected to varying degrees. Therefore, there is a synergistic gain effect between the components of the present invention.

[0115] (II) Nematode lifespan test

[0116] In order to observe the effect of cellular nutrients on lifespan, Caenorhabditis elegans (nematodes) was selected as the object of observation to test its lifespan. The advantages of nematodes are that they have a short lifespan and are easy to control growth conditions. They can be used as a model for studying innate immunity and aging, and have been proven in academia to be an ideal model for solving biological problems in development and neurobiology.

[0117] The culture and processing methods of Caenorhabditis elegans (nematodes) are as follows:

[0118] After suspending the egg-laying nematodes in M9 buffer, pipette them into culture tubes, add lysis solution (5NNaOH and 5% sodium hypochlorite solution) to each tube of nematodes, lyse for 6 minutes, and centrifuge at 3500r / min for 1 minute. Discard the supernatant, wash repeatedly with M9 buffer 4 times, centrifuge again and discard the supernatant. Then transfer the precipitate to the nematode growth medium (NGM), and culture at 20℃ overnight to obtain L1 stage nematode larvae. After washing and centrifuging once with M9 buffer, transfer the nematode larvae to the NGM plate containing Escherichia coli OP50, and culture at 20℃ for 28-30h to obtain synchronized nematodes (i.e. L4 stage nematodes).

[0119] NMG solid culture medium plates were marked for 18 groups, and then 50 μg of each of the cell nutrients prepared in advance were applied to the plates. The blank group was replaced with pure water. Twenty synchronized nematodes were selected from each culture medium, sealed with sealing film, and cultured at 20°C. The number of surviving nematodes, nematodes that died of lack of water by crawling on the wall of the culture dish, and missing nematodes were counted at regular intervals every day. The nematodes that died of lack of water by crawling on the wall of the culture dish and the missing nematodes were removed, and the survival time data of the normally dead nematodes were finally obtained. The test results are shown in Table 3.

[0120] Table 3 Nematode life span test results

[0121] Average life span (days) Change rate Blank Group 21.6 / Example 1 27.2 25.90％ Example 2 25.1 16.20％ Example 3 25 15.70％ Example 4 27.9 29.20％ Example 5 24.8 14.80％ Example 6 27 25％ Example 7 26.8 24.10％ Example 8 27.5 27.30％ Example 9 24.6 13.9％ Comparative Example 1 26 20.40％ Comparative Example 2 22.4 3.70％ Comparative Example 3 23.1 6.90％ Comparative Example 4 25.7 19％ Comparative Example 5 24.6 13.90％ Comparative Example 6 23.8 10.20％ Comparative Example 7 25.6 18.50％ Comparative Example 8 26.8 24.10％

[0122] As shown in Table 3, the cell nutrients of the present invention can increase the life span of nematodes by more than 14%. In Comparative Examples 1-8, although some solutions also have a better effect on extending the life span of nematodes, their growth effect is still somewhat lower than that of the technical solution of the present application.

[0123] In summary, the cell nutrients of the present invention can significantly reduce the products of lipid peroxidation in middle-aged and elderly dogs and cats, improve SOD activity and total antioxidant capacity T-AOC; can help middle-aged and elderly dogs and cats to remove free radicals, play a role in delaying aging, and can reduce the level of inflammation in the body; the cell nutrients can enhance the skin barrier, such as improving the water retention capacity of the skin, and improving the fullness and barrier capacity of skin cells. At the same time, it has a lowering effect on triglycerides, keeps the cardiovascular system of middle-aged and elderly dogs and cats unobstructed, reduces the probability of cardiovascular disease, and has a positive effect on extending the life of middle-aged dogs and cats. In addition, the cell nutrients of the present invention have good thermal stability and can be added in the batching section of puffed staple food, baked staple food, staple food cans, cat strips, freeze-dried, etc.

[0124] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can easily think of various equivalent modifications or replacements within the technical scope disclosed by the present invention, and these modifications or replacements should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention shall be based on the protection scope of the claims.

Claims

1. A cell nutrient for anti-aging and life extension of middle-aged and elderly pets, characterized in that: The invention comprises the following components in parts by weight: 1-10 parts of edible fungus ergothioneine extract, 1-10 parts of krill oil, 0.5-5 parts of collagen peptide and 0.5-5 parts of antioxidant active ingredients.

2. The cell nutrient for anti-aging and life extension of middle-aged and elderly pets as claimed in claim 1, characterized in that: The preparation method of the edible fungus thioneine extract comprises the following steps: (1) Vacuum freeze drying: freeze-drying the edible fungi at a temperature below -50°C and a vacuum degree below 20 Pa, and crushing and screening to obtain edible fungi powder; (2) Extraction and purification of thioneine: edible fungus powder and ethanol solution with a mass concentration of 40-70% are mixed in a mass ratio of 1: (20-30), and extracted in a constant temperature water bath at a water temperature of 45-65° C. for 20-40 min; then microwaved at 60-70° C. for 3-8 min; then ultrasonically extracted for 3-8 min to obtain an extract; the extract is centrifuged at room temperature, the supernatant is collected and purified and filtered to obtain an edible fungus thioneine purified liquid; (3) Drying of thioneine: The purified thioneine solution of edible fungi is concentrated under negative pressure to a wall-hanging state, and then transferred to a vacuum freeze drying box for further freeze drying to obtain crystals, and the crystals are ground to obtain the thioneine extract of edible fungi.

3. The cell nutrient for anti-aging and life extension of middle-aged and elderly pets as claimed in claim 2, characterized in that: In the preparation method of the edible fungus thioneine extract, the purification and filtration step is specifically to first filter with a 300-mesh filter cloth, and then use a polytetrafluoroethylene microporous filter membrane with a pore size of 0.45 μm for purification and filtration.

4. The cell nutrient for anti-aging and life extension of middle-aged and elderly pets as claimed in claim 2, characterized in that: The edible fungus is selected from at least one of boletus, pleurotus eryngii, king oyster mushroom, enoki mushroom, oyster mushroom, shiitake mushroom, Agaricus blazei, Hericium erinaceus, Agrocybe bisporus and the like.

5. The cell nutrient for anti-aging and life extension of middle-aged and elderly pets as claimed in claim 1, characterized in that: The polypeptide molecular weight of the collagen peptide is less than 3000Da.

6. The cell nutrient for anti-aging and life extension of middle-aged and elderly pets as claimed in claim 1, characterized in that: The collagen peptide is selected from one of ray collagen peptide, type II collagen peptide, avian cartilage collagen peptide, shark cartilage collagen peptide and deep-sea fish collagen peptide.

7. The cell nutrient for anti-aging and life extension of middle-aged and elderly pets as claimed in claim 1, characterized in that: The antioxidant active ingredient is selected from at least one of resveratrol, coenzyme Q10, glutathione, vitamin C, anthocyanin, astaxanthin, quercetin, lutein, rosemary extract and grapefruit extract.

8. The cell nutrient for anti-aging and life extension of middle-aged and elderly pets as claimed in claim 1, characterized in that: The total phospholipid content of the krill oil is ≥40%.

9. The method for preparing the cell nutrients for anti-aging and life extension of middle-aged and elderly pets according to any one of claims 1 to 8, characterized in that: The following steps are involved: S1: stirring and mixing the edible fungus thioneine extract, collagen peptide, and antioxidant active ingredients in proportion to make them evenly mixed to obtain a mixed powder; S2: Add the mixed powder into warm water at 40-60°C while stirring to fully dissolve it to obtain aqueous phase A; the mass ratio of the mixed powder to warm water is 1:(3-4); S3: mixing the aqueous phase A and the krill oil, and obtaining an emulsion after high-speed shear emulsification; S4: The obtained emulsion is subjected to high-pressure homogenization treatment to obtain cell nutrients for anti-aging and life-extending of middle-aged and elderly pets.

10. Use of the cell nutrients for anti-aging and life-extending of middle-aged and elderly pets as claimed in any one of claims 1 to 8 in pet food.

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