Cysteine quantitative detection device

Abstract

The invention relates to the technical field of cysteine detection, and provides a cysteine quantitative detection device which comprises a fixed rack, two middle hoppers, a sample storage assembly, an adding assembly, a cleaning assembly, a supporting frame, a detection assembly, a mixing assembly and a controller. The sample storage assemblies are installed on the upper portions of the two middle hoppers, the adding assemblies are installed at the discharging positions of the two middle hoppers, the cleaning assembly is installed on the fixed rack, the supporting frame is installed in the fixed rack, the detection assembly is arranged on the upper portion of the supporting frame, the mixing assembly is installed on the supporting frame, and the detection assembly is installed on the mixing assembly. By means of the technical scheme, the problem that in the prior art, due to residues existing in detection equipment, the accuracy of follow-up detection is reduced is solved.

Description

Technical Field

[0001] The invention relates to the technical field of cysteine ​​detection, and in particular to a cysteine ​​quantitative detection device. Background Art

[0002] L-cysteine ​​is a common amino acid in organisms and one of the sulfur-containing α-amino acids. It turns purple when it meets nitroprusside. It exists in many proteins and glutathione. It can form insoluble thiolates with metal ions such as Ag+, Hg+, and Cu+, namely RSM′, RSM″-SR, with the molecular formula C3H7NO2S. It is a colorless crystal, soluble in water, ethanol, acetic acid and ammonia water, insoluble in ether, acetone, ethyl acetate, benzene, carbon disulfide and carbon tetrachloride. It can be oxidized to cystine by air in neutral and weakly alkaline solutions.

[0003] Since cysteine ​​has multiple physiological functions in organisms, such as anti-oxidation, detoxification, and repair of DNA damage, changes in its concentration directly affect the health of the body. Quantitative detection of cysteine ​​can be used to evaluate whether the body is normal, which is of great value in some clinical application scenarios.

[0004] When detecting cysteine, the appropriate sample type is selected according to specific needs. For blood samples, venous blood is usually required to be drawn. For other liquid samples, such as urine, saliva, etc., the test can be performed by collecting liquid from the corresponding parts. In order to improve the accuracy and sensitivity of the test, it is necessary to perform pretreatment such as removing cell membranes and ribonuclease degradation after sample collection, and then extract it and remove possible interfering substances, so that the extracted samples can be quantitatively tested.

[0005] During the detection process, due to long-term use, some samples that have not been cleaned may remain in the detection equipment. These residues may affect the detection of subsequent samples, resulting in inaccurate test results. In addition, these residues may damage the detection elements, reduce their sensitivity and specificity, lead to misjudgment, and affect the accuracy of the test results. Summary of the invention

[0006] The present invention provides a cysteine ​​quantitative detection device, which solves the problem in the related art that the accuracy of subsequent detection is reduced due to the residue in the detection equipment.

[0007] The technical solution of the present invention is as follows: A cysteine ​​quantitative detection device, comprising: Fixed rack; An intermediate bucket, two intermediate buckets are symmetrically and fixedly mounted on the top of the fixed frame; A sample storage assembly, the sample storage assembly is installed on the upper part of the two middle buckets, and is used to store samples and reagents to be tested; An adding component is installed at the discharge of the two intermediate hoppers, and is used to add samples and reagents to be tested; A cleaning component, which is mounted on the fixed frame and is used to clean the intermediate bucket and the adding component; A support frame, the support frame is installed inside the fixed frame; A detection component, which is arranged on the upper part of the support frame and is used to detect the sample; A mixing component, the mixing component is mounted on the supporting frame, the detection component is mounted on the mixing component, and is used to mix the sample to be detected with the reagent; A controller is fixedly mounted on the fixed frame.

[0008] Based on the above scheme, the sample storage component includes: A connecting sleeve, the upper parts of the two intermediate buckets are detachably mounted with the connecting sleeve; A discharge port, wherein the discharge port is fixedly installed inside the two connecting sleeves; A sample bottle, the sample bottle is detachably mounted on the upper parts of the two connecting sleeves; An opening and closing part is installed on each of the two discharge ports to control the opening and closing of the discharge ports.

[0009] On the basis of the above scheme, the opening and closing part includes: Sliding sleeves, the bottoms of the two discharge ports are both slidably and sealingly mounted with the sliding sleeves; A spring, wherein the two sliding sleeves are fixedly mounted with the spring, the other end of the spring is fixedly connected to the connecting sleeve, and the discharge port is located inside the spring; A conical plug block, the top of each of the two discharge ports is provided with the conical plug block, and the conical plug block is adapted to the top of the discharge port; A connecting rod is fixedly mounted on the two sliding sleeves, and the top end of the connecting rod is fixedly connected to the conical plug block.

[0010] Based on the above solution, the additional components include: A first electric cylinder, the lower parts of the two intermediate buckets are fixedly mounted with the first electric cylinder, and the output end of the first electric cylinder is adapted to the bottom of the sliding sleeve; A discharge pipe, the bottoms of the two intermediate buckets are connected to the discharge pipe; A first control valve is fixedly installed on the two discharge pipes.

[0011] Based on the above solution, the cleaning component includes: A water tank, the water tank is fixedly mounted on the fixed frame; A water pump, wherein the water pump is fixedly mounted on the water tank; A water inlet pipe, the water inlet pipe being connected between an input end of the water pump and the water tank; An air pump, the air pump is fixedly mounted on the water tank; An exhaust pipe, the output end of the air pump is connected to the exhaust pipe; A cleaning part is installed on the middle bucket and is used for cleaning the middle bucket and the discharge pipe.

[0012] On the basis of the above scheme, the cleaning unit comprises: A one-way valve, wherein the output end of the water pump and the output end of the exhaust pipe are both connected with the one-way valve; A mixing shell, the mixing shell is arranged on the upper part of the water pump, and the mixing shell is communicated with the two one-way valves respectively; A drainage pipe, the two middle buckets are eccentrically connected with the drainage pipe; A connecting pipe, the top of the mixing housing is connected to the connecting pipe, and the two drain pipes are both connected to the connecting pipe; A second control valve is fixedly installed on the two drainage pipes.

[0013] Based on the above solution, the detection component includes: A load-bearing frame, the load-bearing frame is arranged on the upper part of the support frame; A fixing block, the fixing block is symmetrically arranged inside the supporting frame; A second electric cylinder, the second electric cylinder is fixedly mounted on both the front and rear sides of the carrier frame, and the output end of the second electric cylinder is fixedly connected to the fixing block; Photoelectric sensors, the photoelectric sensors are fixedly mounted on the left and right sides of the carrier, and the two photoelectric sensors are symmetrically arranged relative to the carrier; A quantitative unit is installed inside the carrier and is used to determine the quality of samples and reagents.

[0014] On the basis of the above scheme, the quantitative part includes: A weighing sensor, wherein the weighing sensor is fixedly mounted on the inner bottom wall of the bearing frame; A loading plate, the loading plate is fixedly mounted on the weighing sensor; A detection cup is detachably arranged on the carrier plate.

[0015] On the basis of the above scheme, the mixing assembly comprises: Slide rack, two slide racks are symmetrically and fixedly mounted on the bottom of the carrier rack; Slide grooves, two slide grooves are symmetrically provided on the two slide racks; A slider, each of the slide grooves is slidably mounted with the slider; A slide rail, wherein the support frame is symmetrically and fixedly mounted with the slide rail, the slide groove is vertically arranged with the slide rail, and the slider is slidably matched with the slide rail; A driving unit is installed on the support frame and is used to drive the bearing frame to move.

[0016] On the basis of the above scheme, the driving unit comprises: A connecting plate, the connecting plate is fixedly mounted on the bottom of the supporting frame; A motor, wherein the motor is fixedly mounted on the support frame; A driving gear, the driving gear being fixedly mounted at an output end of the motor; Driven gears: two driven gears are symmetrically and rotatably mounted on the support frame, and both driven gears are meshed with the driving gear; A rotating block, the upper parts of the two driven gears are both rotating and eccentrically mounted with the rotating block, and the two rotating blocks are both rotatably connected to the connecting plate; Wherein, the two rotating blocks are arranged symmetrically relative to the connecting plate.

[0017] The working principle and beneficial effects of the present invention are: 1. In the present invention, as the output end of the first electric cylinder is pushed out, the sliding sleeve can be pushed to move, so that the sample and reagent in the sample bottle are discharged into the corresponding intermediate bucket. Under the cooperation of the controller, the amount of the sample and reagent in the intermediate bucket entering the discharge pipe is controlled by the first control valve, and then the sample and reagent are discharged into the detection cup through the discharge pipe, thereby ensuring that the quality of each sample and reagent is the same, thereby ensuring the comparability of the results of each test on the same sample.

[0018] 2. In the present invention, the connection between the sample bottle and the connecting sleeve, and between the connecting sleeve and the intermediate bucket are all threaded connections. At the same time, the cooperation between the detection cup and the loading plate is also convenient for disassembly and assembly, so that the sample bottle and the detection cup can be easily detected, thereby improving the quality and efficiency of cleaning.

[0019] 3. In the present invention, when the intermediate bucket and the discharge pipe are cleaned, after the water pump delivers the cleaning liquid into the mixing shell, the air pump also inflates the mixing shell through the exhaust pipe. At this time, the air and liquid in the mixing shell are mixed, and then discharged into the connecting pipe and finally into the drain pipe. Since the connecting position between the drain pipe and the intermediate bucket is eccentrically arranged relative to the axial position of the intermediate bucket, the cleaning power of the water flow entering the intermediate bucket and the side wall of the intermediate bucket is improved, so that the intermediate bucket is cleaned more thoroughly. Through the setting of the one-way valve, it can be ensured that the water flow and air in the mixing shell will not affect the water pump and the air pump, thereby improving the safety during operation.

[0020] 4. In the present invention, by setting up the sample storage component, it is not only convenient to perform multiple tests on the same sample, but also the sample bottle can be conveniently cleaned after the test. By setting up the adding component and the detection component, the reagent and sample entering the detection cup can be accurately weighed, thereby quantifying the reagent and sample, improving the precision and accuracy of the test. By setting up the cleaning component, after the test, the place where the sample and reagent flow through can be quickly and thoroughly cleaned, reducing the generation of residues and greatly improving the accuracy of the test. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0022] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a schematic diagram of the overall structure from another angle in the present invention; Figure 3 This is a schematic diagram of the overall structure from another angle in the present invention; Figure 4 It is a cross-sectional structural schematic diagram of the cooperation between the sample storage component and the addition component in the present invention; Figure 5 is a schematic cross-sectional structural diagram of the sample storage assembly of the present invention; Figure 6 It is a schematic diagram of the cross-sectional structure of the opening and closing part of the present invention; Figure 7 It is a schematic diagram of the structure of the cleaning component in the present invention; Figure 8 It is a schematic diagram of the structure of the detection component and the mixing component in the present invention; Fig. 9 It is a schematic cross-sectional structural diagram of the detection component in the present invention; Fig.10 It is a cross-sectional structural schematic diagram of the detection component and the mixing component in the present invention; Fig.11is a schematic cross-sectional structural diagram of the mixing assembly in the present invention; Fig.12 It is a cross-sectional structural schematic diagram of the cooperation between the driving part and the supporting frame in the present invention; Fig.13 It is a schematic diagram of the structure of the driving part in the present invention.

[0023] The numbers in the figure represent: 1, fixed frame; 2, middle bucket; 3, support frame; 4, controller; 5, connecting sleeve; 6, discharge port; 7, sample bottle; 8, sliding sleeve; 9, spring; 10, tapered plug; 11, connecting rod; 12, first electric cylinder; 13, discharge pipe; 14, first control valve; 15, water tank; 16, water pump; 17, water inlet pipe; 18, air pump; 19, exhaust pipe; 20, one-way valve; 21 , mixing shell; 22, drain pipe; 23, connecting pipe; 24, second control valve; 25, load frame; 26, fixed block; 27, second electric cylinder; 28, photoelectric sensor; 29, weighing sensor; 30, loading plate; 31, detection cup; 32, slide; 33, slide groove; 34, slider; 35, slide rail; 36, connecting plate; 37, motor; 38, driving gear; 39, driven gear; 40, rotating block. DETAILED DESCRIPTION

[0024] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] like Figures 1 to 13 As shown, this embodiment proposes a cysteine ​​quantitative detection device, including a fixed frame 1, an intermediate bucket 2, a sample storage component, an addition component, a cleaning component, a support frame 3, a detection component, a mixing component and a controller 4, the fixed frame 1 has two intermediate buckets 2 symmetrically and fixedly installed on the top, the two intermediate buckets 2 are both installed with the sample storage component on the top for storing samples and reagents to be detected, the sample storage component includes a connecting sleeve 5, a discharge port 6, a sample bottle 7 and an opening and closing part, the connecting sleeves 5 are detachably installed on the top of the two intermediate buckets 2, the discharge port 6 is fixedly installed inside the two connecting sleeves 5, the sample bottle 7 is detachably installed on the top of the two connecting sleeves 5, and the opening and closing part is installed on the two discharge ports 6 for controlling the opening and closing of the discharge port 6.

[0026] Specifically, when detecting cysteine ​​in the collected sample, the sample to be detected is first pretreated to remove or reduce other interfering substances that may affect the detection result of cysteine, and then poured into one of the sample bottles 7. At the same time, the reagent is poured into the other sample bottle 7, and then the connecting sleeve 5 is respectively installed on the two sample bottles 7. The connection between the connecting sleeve 5 and the sample bottle 7 preferably adopts a threaded connection structure. After the connecting sleeve 5 is installed on the sample bottle 7, due to the setting of the opening and closing part, it can be ensured that the substance in the sample bottle 7 will not spill out. Then the two sample bottles 7 are turned upside down so that the discharge port 6 is set downward, and then the two sample bottles 7 are respectively moved to the top of the corresponding intermediate bucket 2. At this time, the corresponding sample bottle 7 can be connected to the intermediate bucket 2 through the setting of the connecting sleeve 5, wherein the connection between the connecting sleeve 5 and the intermediate bucket 2 is The connection method is also preferably implemented by a threaded connection structure. After the sample bottle 7 is installed, the substances in the two sample bottles 7 are taken out respectively through the setting of the adding component. At this time, through the cooperation of the detection component, under the control of the controller 4, the taken out samples and reagents are quantitatively added, and then the reagents and samples are mixed together through the mixing component, and then they can be detected by the detection component. After the end, the sample in the detection component is disposed of, and the measured absorbance value is substituted into the colorimetric equation to calculate the concentration of the sample. In order to ensure the accuracy and reliability of the results, multiple independent tests are usually required in the same sample, which will help reduce experimental errors and improve the accuracy of the test. At this time, repeat the above steps, and record and compare the values ​​measured each time to ensure the accuracy and consistency of the measurement results.

[0027] The above, such as Figure 4 , Figure 6 As shown, the opening and closing part includes a sliding sleeve 8, a spring 9, a conical plug block 10 and a connecting rod 11. The sliding sleeve 8 is slidably and sealingly installed on the bottom of the two discharge ports 6. The spring 9 is fixedly installed on the two sliding sleeves 8. The other end of the spring 9 is fixedly connected to the connecting sleeve 5. The discharge port 6 is located inside the spring 9. The conical plug block 10 is arranged on the top of the two discharge ports 6. The conical plug block 10 is adapted to the top of the discharge port 6. The connecting rod 11 is fixedly installed on the two sliding sleeves 8. The top of the connecting rod 11 is fixedly connected to the conical plug block 10.

[0028] Specifically, under normal conditions, the spring 9 is in a compressed state. At this time, the spring 9 applies pressure to the sliding sleeve 8. The sliding sleeve 8 will pull the conical plug block 10 through the setting of the connecting rod 11, so that the bottom of the conical plug block 10 is tightly fitted with the top of the discharge port 6, so that the material in the sample bottle 7 will not spill out from the discharge port 6. When the material in the sample bottle 7 needs to be taken out, a pressure opposite to the pressure of the spring 9 is applied to the sliding sleeve 8, so that the spring 9 is compressed and the sliding sleeve 8 moves on the discharge port 6. At this time, the sliding sleeve 8 lifts up the conical plug block 10 through the connecting rod 11, so that the material in the sample bottle 7 flows from the discharge port 6 into the corresponding intermediate bucket 2.

[0029] like Figure 4 As shown, the adding components are installed at the discharge points of the two intermediate buckets 2 for adding samples and reagents to be tested, and the adding components include a first electric cylinder 12, a discharge pipe 13 and a first control valve 14. The first electric cylinder 12 is fixedly installed at the lower part of the two intermediate buckets 2, and the output end of the first electric cylinder 12 is adapted to the bottom of the sliding sleeve 8. The bottoms of the two intermediate buckets 2 are connected to the discharge pipe 13, and the first control valve 14 is fixedly installed on the two discharge pipes 13.

[0030] Specifically, when it is necessary to discharge the sample or reagent in the sample bottle 7 into the intermediate bucket 2, the corresponding first electric cylinder 12 is started, and the output end of the first electric cylinder 12 abuts against the bottom of the sliding sleeve 8. As the output end of the first electric cylinder 12 is pushed out, the sliding sleeve 8 can be pushed to move, so that the sample and reagent in the sample bottle 7 are discharged into the corresponding intermediate bucket 2. With the cooperation of the controller 4, the first control valve 14 is used to control the amount of sample and reagent in the intermediate bucket 2 entering the discharge pipe 13, and then the sample and reagent are discharged into the detection cup 31 through the discharge pipe 13, thereby ensuring that the quality of each sample and reagent is the same, thereby ensuring the comparability of the results of each test on the same sample.

[0031] like Figure 7 As shown, the cleaning component is installed on the fixed frame 1, and is used for cleaning the intermediate bucket 2 and the adding component. The cleaning component includes a water tank 15, a water pump 16, a water inlet pipe 17, an air pump 18, an exhaust pipe 19 and a cleaning part. The water tank 15 is fixedly installed on the fixed frame 1, the water pump 16 is fixedly installed on the water tank 15, the water inlet pipe 17 is connected between the input end of the water pump 16 and the water tank 15, the air pump 18 is fixedly installed on the water tank 15, and the output end of the air pump 18 is connected to the exhaust pipe 19. The cleaning part is installed on the intermediate bucket 2, and is used for cleaning the intermediate bucket 2 and the discharge pipe 13.

[0032] Specifically, after each inspection, it is necessary to retract the first electric cylinder 12 so that the conical plug 10 is re-engaged with the discharge port 6. At this time, the intermediate bucket 2 and the discharge pipe 13 can be cleaned. At this time, the water pump 16 and the air pump 18 are started respectively. The water pump 16 draws out the cleaning liquid in the water tank 15 through the water inlet pipe 17, and then discharges it into the cleaning part. At the same time, the air pump 18 will pump air into the cleaning part through the exhaust pipe 19. At this time, the water flow and the air are mixed in the cleaning part. At this time, a certain amount of bubbles are mixed in the water flow. These water flows mixed with bubbles flush the intermediate bucket 2. At this time, the first control valve 14 is in an open state, and the water flow entering the intermediate bucket 2 will be discharged through the discharge pipe 13, thereby thoroughly cleaning the residual reagents and samples in the intermediate bucket 2 and the discharge pipe 13, and then the water pump 16 is turned off. At this time, the gas entering the intermediate bucket 2 and the discharge pipe 13 will blow out the residual cleaning liquid therein, so that the intermediate bucket 2 and the discharge pipe 13 are kept clean, and the next inspection can be started.

[0033] The above, such as Figure 7 As shown, the cleaning part includes a one-way valve 20, a mixing shell 21, a drain pipe 22, a connecting pipe 23 and a second control valve 24. The output end of the water pump 16 and the output end of the exhaust pipe 19 are both connected with the one-way valve 20. The mixing shell 21 is arranged on the upper part of the water pump 16. The mixing shell 21 is respectively connected with the two one-way valves 20. The two middle buckets 2 are eccentrically connected with the drain pipe 22. The top of the mixing shell 21 is connected with the connecting pipe 23. The two drain pipes 22 are both connected with the connecting pipe 23. The second control valve 24 is fixedly installed on the two drain pipes 22.

[0034] Specifically, after the water pump 16 delivers the cleaning liquid into the mixing shell 21, at the same time, the air pump 18 also inflates the mixing shell 21 through the exhaust pipe 19. At this time, the air and liquid in the mixing shell 21 are mixed, and then discharged into the connecting pipe 23, and finally enter the drain pipe 22. At this time, the second control valve 24 is in an open state. Since the connecting position between the drain pipe 22 and the intermediate bucket 2 is eccentrically arranged relative to the axial position of the intermediate bucket 2, the cleaning force of the water flow entering the intermediate bucket 2 and the side wall of the intermediate bucket 2 is improved, so that the intermediate bucket 2 is cleaned more thoroughly. Through the setting of the one-way valve 20, it can be ensured that the water flow and air in the mixing shell 21 will not affect the water pump 16 and the air pump 18, thereby improving the safety during operation.

[0035] like Figure 8 , Fig. 9As shown, the support frame 3 is installed inside the fixed frame 1, and the detection component is arranged on the upper part of the support frame 3 for detecting samples. The detection component includes a carrier frame 25, a fixed block 26, a second electric cylinder 27, a photoelectric sensor 28 and a quantitative unit. The carrier frame 25 is arranged on the upper part of the support frame 3, and the fixed block 26 is symmetrically arranged inside the carrier frame 25. The second electric cylinder 27 is fixedly installed on the front and rear sides of the carrier frame 25, and the output end of the second electric cylinder 27 is fixedly connected to the fixed block 26. The photoelectric sensor 28 is fixedly installed on the left and right sides of the carrier frame 25, and the two photoelectric sensors 28 are symmetrically arranged relative to the carrier frame 25. The quantitative unit is installed inside the carrier frame 25 to determine the quality of samples and reagents.

[0036] Specifically, when detecting cysteine ​​in the sample, the sample is first poured into the quantitative part to determine the mass of the sample, and then the reagent of the determined mass is poured into the quantitative part. Before observing the reagent and the sample to be detected, the sample and the reagent need to be mixed evenly. At this time, the second electric cylinder 27 is started, and the second electric cylinder 27 drives the fixed block 26 to move. Through the setting of the fixed block 26, spillage during the mixing process of the reagent and the sample can be avoided. After the mixing is completed, the photoelectric sensor 28 is started to observe the sample, and then the collected data is sent to the controller 4, and the data can be analyzed.

[0037] The above, such as Fig. 9 As shown, the quantitative part includes a weighing sensor 29, a loading plate 30 and a detection cup 31. The weighing sensor 29 is fixedly mounted on the inner bottom wall of the carrier 25, the loading plate 30 is fixedly mounted on the weighing sensor 29, and the detection cup 31 is detachably arranged on the loading plate 30.

[0038] Specifically, after pouring the sample in the sample bottle 7 into the detection cup 31, the mass of the sample entering the detection cup 31 can be determined by the setting of the weighing sensor 29. When the sample mass reaches the requirement, the weighing sensor 29 can be set. The weighing sensor 29 is provided with a signal transmitter, and the first control valve 14 is provided with a matching signal receiver. When the weighing sensor 29 is under the action of the controller 4, the mass of the sample in the detection cup 31 is detected in real time. After the sample mass reaches the set value, the first control valve 14 can be closed, and then the same steps are adopted to quantitatively add the reagent in another sampling bottle to the detection cup 31.

[0039] like Fig.10 , Fig.11As shown, the mixing component is installed on the supporting frame 3, the detecting component is installed on the mixing component, and is used to mix the sample and reagent to be detected, the controller 4 is fixedly installed on the fixed frame 1, the mixing component includes a slide 32, a slide groove 33, a slider 34, a slide rail 35 and a driving unit, the bottom of the supporting frame 25 is symmetrically and fixedly installed with two slides 32, two slide grooves 33 are symmetrically provided on the two slides 32, each of the slide grooves 33 is slidably installed with the slider 34, the supporting frame 3 is symmetrically and fixedly installed with the slide rail 35, the slide groove 33 and the slide rail 35 are vertically arranged, the slider 34 and the slide rail 35 are slidably matched, and the driving unit is installed on the supporting frame 3, and is used to drive the supporting frame 25 to move.

[0040] Specifically, after a certain amount of reagent and sample are poured into the detection cup 31, the driving unit is started, and the setting of the driving unit drives the carrier 25 to move. At this time, the carrier 25 drives the slide 32 to move back and forth along the direction of the slide rail 35, thereby driving the slider 34 to move back and forth on the slide rail 35. At the same time, the slider 34 and the slide groove 33 also reciprocate, so that the carrier 25 can be rotated, and the carrier 25 itself does not rotate, thereby reducing the possibility of the sample spilling out of the detection cup 31, and the sample and the reagent are evenly mixed together, and then the detection can be started.

[0041] The above, such as Fig.12 , Fig.13 As shown, the driving part includes a connecting plate 36, a motor 37, a driving gear 38, a driven gear 39 and a rotating block 40. The connecting plate 36 is fixedly mounted on the bottom of the carrier frame 25, the motor 37 is fixedly mounted on the support frame 3, the driving gear 38 is fixedly mounted on the output end of the motor 37, and two driven gears 39 are symmetrically and rotatably mounted on the support frame 3. Both of the two driven gears 39 are meshed with the driving gear 38. The upper parts of the two driven gears 39 are both rotatably and eccentrically mounted with the rotating blocks 40, and the two rotating blocks 40 are both rotatably connected to the connecting plate 36, wherein the two rotating blocks 40 are symmetrically arranged relative to the connecting plate 36.

[0042] Specifically, when driving the carrier 25 to move, the motor 37 is started, the motor 37 drives the driving gear 38 to rotate, the driving gear 38 drives the driven gear 39 to rotate, the driven gear 39 drives the rotating block 40 to rotate, thereby driving the connecting plate 36 to move, and the setting of the two rotating blocks 40 improves the stability when mixing samples and reagents.

[0043] In summary, when detecting cysteine ​​in the collected samples, the sample to be detected is first pretreated to remove or reduce other interfering substances that may affect the detection result of cysteine, and then poured into one of the sample bottles 7. At the same time, the reagent is poured into the other sample bottle 7, and then the connecting sleeve 5 is respectively installed on the two sample bottles 7. The connection between the connecting sleeve 5 and the sample bottle 7 first adopts a threaded connection structure.

[0044] Under normal conditions, the spring 9 is in a compressed state. At this time, the spring 9 applies pressure to the sliding sleeve 8. The sliding sleeve 8 will pull the conical plug block 10 through the setting of the connecting rod 11, so that the bottom of the conical plug block 10 is tightly fitted with the top of the discharge port 6, so that the material in the sample bottle 7 will not spill out from the discharge port 6. After installing the connecting sleeve 5 on the sample bottle 7, the two sample bottles 7 are turned upside down so that the discharge port 6 is set downward, and then the two sample bottles 7 are respectively moved to the top of the corresponding intermediate bucket 2. At this time, the corresponding sample bottle 7 can be connected to the intermediate bucket 2 through the setting of the connecting sleeve 5, wherein the connection method between the connecting sleeve 5 and the intermediate bucket 2 is also preferably realized by a threaded connection structure.

[0045] After installing the sample bottle 7, put the clean test cup 31 on the loading plate 30 in the carrier 25, and then add the reagents and samples in the two sample bottles 7 into the test cup 31 in a quantitative manner. At this time, pour the sample into the test cup 31 first, start the corresponding first electric cylinder 12, and the output end of the first electric cylinder 12 abuts against the bottom of the sliding sleeve 8. As the output end of the first electric cylinder 12 is pushed out, the sliding sleeve 8 can be pushed to move. By applying a pressure opposite to the pressure of the spring 9 to the sliding sleeve 8, the spring 9 is compressed, and the sliding sleeve 8 moves on the discharge port 6. At this time, the sliding sleeve 8 lifts up the conical plug 10 through the connecting rod 11, so that the sample in the sample bottle 7 flows into the corresponding intermediate bucket 2 from the discharge port 6, and then the first control valve 14 is started to allow the sample entering the corresponding intermediate bucket 2 to enter the corresponding discharge pipe 13.

[0046] After the sample in the sample bottle 7 is poured into the detection cup 31, the mass of the sample entering the detection cup 31 can be determined by the setting of the weighing sensor 29. When the sample mass reaches the requirement, the weighing sensor 29 can be set. The weighing sensor 29 is provided with a signal transmitter, and the first control valve 14 is provided with a matching signal receiver. When the weighing sensor 29 is under the action of the controller 4, the mass of the sample in the detection cup 31 is detected in real time. After the sample mass reaches the set value, the first control valve 14 can be closed. With the cooperation of the controller 4, the first control valve 14 is used to control the amount of sample and reagent in the middle bucket 2 entering the discharge pipe 13, thereby ensuring that the mass of each sample and reagent is the same, and ensuring the comparability of the results of each test on the same sample. Then, the same steps are adopted to add the reagent in another sampling bottle to the detection cup 31 in a quantitative manner.

[0047] Specifically, after a certain amount of reagent and sample are poured into the detection cup 31, the second electric cylinder 27 is started, and the second electric cylinder 27 drives the fixed block 26 to move. By setting the fixed block 26, the reagent and sample can be prevented from spilling out during the mixing process. Then the motor 37 is started, and the motor 37 drives the driving gear 38 to rotate, and the driving gear 38 drives the driven gear 39 to rotate, and the driven gear 39 drives the rotating block 40 to rotate, thereby driving the connecting plate 36 to move, and the carrier 25 can be driven to move. At this time, the carrier 25 drives the slide 32 to reciprocate along the direction of the slide rail 35, thereby driving the slider 34 to reciprocate on the slide rail 35. At the same time, the slider 34 and the slide groove 33 also reciprocate, so that the carrier 25 can be rotated, and the carrier 25 itself does not rotate, thereby reducing the possibility of the sample spilling out of the detection cup 31, and the sample and the reagent are evenly mixed together, and the photoelectric sensor 28 can be started to observe the sample, and then the collected data is sent to the controller 4, and the data can be analyzed.

[0048] After the detection is completed and the corresponding data is collected, the measured absorbance value is substituted into the colorimetric equation, the concentration of the sample is calculated, and a record is made. After the detection, the first electric cylinder 12 needs to be retracted to make the conical plug 10 abut against the discharge port 6 again. At this time, the intermediate bucket 2 and the discharge pipe 13 can be cleaned. At this time, the water pump 16 and the air pump 18 are started respectively. The water pump 16 draws the cleaning liquid out of the water tank 15 through the water inlet pipe 17. When the water pump 16 sends the cleaning liquid into the mixing shell 21, at the same time, the air pump 18 also inflates the mixing shell 21 through the exhaust pipe 19. At this time, the air and liquid in the mixing shell 21 are mixed. At this time, a certain amount of bubbles are mixed in the water flow, and then discharged into the connecting pipe 23, and finally into the drain pipe 22. At this time, the second control valve 24 is in an open state. The connecting position between the drain pipe 22 and the middle bucket 2 is eccentrically arranged relative to the axial position of the middle bucket 2. The water flow mixed with bubbles flushes the middle bucket 2, thereby improving the cleaning power of the water flow entering the middle bucket 2 and the side wall of the middle bucket 2. At this time, the first control valve 14 is in an open state, and the water flow entering the middle bucket 2 will be discharged through the discharge pipe 13, thereby thoroughly cleaning the reagents and samples remaining in the middle bucket 2 and the discharge pipe 13, and then the water pump 16 is turned off. At this time, the gas entering the middle bucket 2 and the discharge pipe 13 will blow out the remaining cleaning liquid therein, thereby keeping the middle bucket 2 and the discharge pipe 13 clean. Through the setting of the one-way valve 20, it can be ensured that the water flow and air in the mixing shell 21 will not affect the water pump 16 and the air pump 18, thereby improving the safety during operation.

[0049] These cleaning liquids will be discharged into the detection cup 31. At this time, the fixed frame 1 is opened, the second electric cylinder 27 is retracted, the detection cup 31 is taken out, the waste in the detection cup 31 is disposed of, and then the detection cup 31 is thoroughly cleaned. In order to ensure the accuracy and reliability of the results, multiple independent tests are usually required on the same sample, which will help reduce experimental errors and improve the accuracy of the test. At this time, repeat the above steps and record and compare the values ​​measured each time to ensure the accuracy and consistency of the measurement results.

[0050] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A cysteine ​​quantitative detection device, characterized in that: include: Fixed frame (1); An intermediate bucket (2), two intermediate buckets (2) being symmetrically and fixedly mounted on the top of the fixed frame (1); A sample storage assembly, the sample storage assembly being installed on the upper part of the two middle buckets (2) and being used for storing samples and reagents to be tested; An adding component, the adding component is installed at the discharge of the two intermediate buckets (2) and is used for adding samples and reagents to be tested; A cleaning component, the cleaning component is mounted on the fixed frame (1) and is used to clean the intermediate bucket (2) and the adding component; A support frame (3), the support frame (3) being installed inside the fixed frame (1); A detection component, the detection component is arranged on the upper part of the support frame (3) and is used to detect the sample; A mixing component, the mixing component is mounted on the support frame (3), the detection component is mounted on the mixing component, and is used to mix the sample to be detected with the reagent; A controller (4), wherein the controller (4) is fixedly mounted on the fixed frame (1).

2. A cysteine ​​quantitative detection device according to claim 1, characterized in that: The sample storage assembly comprises: A connecting sleeve (5), the connecting sleeve (5) being detachably mounted on the upper parts of the two intermediate buckets (2); A discharge port (6), wherein the discharge port (6) is fixedly installed inside the two connecting sleeves (5); A sample bottle (7), the sample bottle (7) being detachably mounted on the upper parts of the two connecting sleeves (5); An opening and closing part is installed on each of the two discharge ports (6) and is used to control the opening and closing of the discharge ports (6).

3. A cysteine ​​quantitative detection device according to claim 2, characterized in that: The opening and closing part comprises: A sliding sleeve (8), the sliding sleeve (8) being slidably and sealingly mounted on the bottom of the two discharge ports (6); A spring (9), wherein the spring (9) is fixedly mounted on each of the two sliding sleeves (8), the other end of the spring (9) is fixedly connected to the connecting sleeve (5), and the discharge port (6) is located inside the spring (9); A conical plug block (10), the top of each of the two discharge ports (6) being provided with the conical plug block (10), the conical plug block (10) being adapted to fit the top of the discharge port (6); A connecting rod (11), the connecting rod (11) being fixedly mounted on the two sliding sleeves (8), and the top end of the connecting rod (11) being fixedly connected to the conical plug block (10).

4. A cysteine ​​quantitative detection device according to claim 3, characterized in that: The added components include: a first electric cylinder (12), the first electric cylinder (12) being fixedly mounted on the lower part of the two intermediate buckets (2), the output end of the first electric cylinder (12) being adapted to the bottom of the sliding sleeve (8); A discharge pipe (13), the bottoms of the two intermediate buckets (2) are both connected to the discharge pipe (13); A first control valve (14), wherein the first control valve (14) is fixedly mounted on each of the two discharge pipes (13).

5. A cysteine ​​quantitative detection device according to claim 4, characterized in that: The cleaning component comprises: A water tank (15), the water tank (15) being fixedly mounted on the fixed frame (1); a water pump (16), the water pump (16) being fixedly mounted on the water tank (15); A water inlet pipe (17), the water inlet pipe (17) being connected between an input end of the water pump (16) and the water tank (15); an air pump (18), the air pump (18) being fixedly mounted on the water tank (15); an exhaust pipe (19), wherein the output end of the air pump (18) is connected to the exhaust pipe (19); A cleaning unit, the cleaning unit is mounted on the middle bucket (2) and is used to clean the middle bucket (2) and the discharge pipe (13).

6. A cysteine ​​quantitative detection device according to claim 5, characterized in that: The cleaning unit comprises: a one-way valve (20), wherein the output end of the water pump (16) and the output end of the exhaust pipe (19) are both connected to the one-way valve (20); A mixing housing (21), the mixing housing (21) being arranged on an upper portion of the water pump (16), the mixing housing (21) being respectively connected to the two one-way valves (20); A drainage pipe (22), the two intermediate buckets (2) being eccentrically connected to the drainage pipe (22); A connecting pipe (23), the top of the mixing housing (21) is connected to the connecting pipe (23), and the two drainage pipes (22) are both connected to the connecting pipe (23); A second control valve (24), wherein the second control valve (24) is fixedly mounted on each of the two drainage pipes (22).

7. A cysteine ​​quantitative detection device according to claim 6, characterized in that: The detection component comprises: A bearing frame (25), the bearing frame (25) being arranged on an upper portion of the supporting frame (3); A fixing block (26), the fixing block (26) being symmetrically arranged inside the supporting frame (25); a second electric cylinder (27), the second electric cylinder (27) being fixedly mounted on both the front and rear sides of the carrier frame (25), the output end of the second electric cylinder (27) being fixedly connected to the fixing block (26); Photoelectric sensors (28), the photoelectric sensors (28) being fixedly mounted on both left and right sides of the carrier (25), and the two photoelectric sensors (28) being symmetrically arranged relative to the carrier (25); A quantification unit, the quantification unit is installed inside the carrier (25) and is used to determine the mass of the sample and the reagent.

8. A cysteine ​​quantitative detection device according to claim 7, characterized in that: The quantitative unit comprises: A weighing sensor (29), the weighing sensor (29) being fixedly mounted on the inner bottom wall of the supporting frame (25); A loading plate (30), the loading plate (30) being fixedly mounted on the weighing sensor (29); A detection cup (31), wherein the detection cup (31) is detachably arranged on the object carrier plate (30).

9. A cysteine ​​quantitative detection device according to claim 8, characterized in that: The mixing assembly comprises: Slide racks (32), two slide racks (32) being symmetrically and fixedly mounted on the bottom of the carrier frame (25); A slide groove (33), wherein two slide grooves (33) are symmetrically provided on each of the two slide racks (32); A slider (34), each of the slide grooves (33) being slidably mounted with the slider (34); A slide rail (35), the support frame (3) is symmetrically and fixedly mounted with the slide rail (35), the slide groove (33) and the slide rail (35) are vertically arranged, and the slider (34) and the slide rail (35) are slidably matched; A driving unit, the driving unit is mounted on the support frame (3) and is used to drive the bearing frame (25) to move.

10. A cysteine ​​quantitative detection device according to claim 9, characterized in that: The driving unit comprises: A connecting plate (36), the connecting plate (36) being fixedly mounted on the bottom of the supporting frame (25); A motor (37), wherein the motor (37) is fixedly mounted on the support frame (3); A driving gear (38), the driving gear (38) being fixedly mounted on an output end of the motor (37); A driven gear (39), wherein two driven gears (39) are symmetrically and rotatably mounted on the support frame (3), and both of the driven gears (39) are meshed with the driving gear (38); A rotating block (40), the upper parts of the two driven gears (39) are both rotatable and eccentrically mounted with the rotating block (40), and the two rotating blocks (40) are both rotatably connected to the connecting plate (36); The two rotating blocks (40) are symmetrically arranged relative to the connecting plate (36).

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