Intestinal flora targeted formula food capable of improving immunity and preparation method of intestinal flora targeted formula food

Abstract

The invention discloses an intestinal flora targeted formula food for improving immunity and a preparation method thereof. The intestinal flora targeted formula food comprises the following components in parts by mass: 75-85 parts of raw milk, 1-5 parts of white granulated sugar, 5-10 parts of inulin, 5-10 parts of oat, 5-10 parts of resistant dextrin and 3-8 parts of xylooligosaccharide. The fermentation strain is a Kefir strain, and the addition amount of the Kefir strain is 0.3-1.0% of the mass of the raw milk. According to the formula food disclosed by the invention, prebiotics such as the inulin rich in dietary fibers, the resistant dextrin and the like are used, and the probiotics are added, so that intestinal tract movement can be promoted, intestinal tract microorganism balance can be favorably maintained, the structure and diversity of intestinal flora can be regulated, the expression of sugar degradation carbohydrate active enzyme coded by microorganisms can be increased, and the production of short-chain fatty acid can be promoted; inflammation signals and vitality are reduced, and the immunity of the organism is improved; the cost is low, and the product is suitable for long-term consumption of vast patients with reduced resistance and low immunity.

Description

Technical Field

[0001] The present invention relates to a formula food for improving human immunity and a preparation method thereof, and in particular to an intestinal flora targeted formula food for improving immunity and a preparation method thereof. Background Art

[0002] Immunity is a defense mechanism of the human body. Its main function is to protect the body from invasion by foreign pathogens such as bacteria and viruses, and to remove abnormal cells in the body, such as cancerous cells, aging cells, damaged cells, etc. This defense is composed of immune cells and tissues, which work together to maintain the body's health. When immunity is too low, the human body is susceptible to various infections, and the risk of tumors may also increase. On the contrary, excessive immunity may also lead to immune system disorders, thereby causing autoimmune diseases. Therefore, maintaining a balanced and orderly state of immunity is essential for human health. In order to maintain the normal functioning of immunity, it is recommended to maintain a healthy lifestyle, such as a balanced diet, moderate exercise, keeping a good mood, and considering appropriate supplementation of probiotics. At the same time, maintaining healthy living habits is also very important for maintaining good immunity.

[0003] There is a close relationship between intestinal flora and body immunity. The intestine is one of the largest immune organs in the human body, and about 70% of immune cells are distributed in the intestine. The balance of intestinal flora plays an important role in the normal function of the immune system. Probiotics in the intestine, such as bifidobacteria and lactobacilli, can synthesize a variety of vitamins needed by the human body, help the absorption and digestion of food, promote the body's metabolism, and inhibit the growth of pathogenic bacteria and promote the growth of beneficial flora. These beneficial flora can also maintain the health of the intestine by producing antibacterial substances and inhibiting the growth of harmful bacteria. The intestinal microbiota can affect the maturation and function of immune cells, especially the development and differentiation of T cells. For example, intestinal microorganisms can induce intestinal dendritic cells to transport microbial antigens from the intestine to the thymus, and then induce the expansion of microbiota-specific T cells, enhancing the ability to recognize intestinal microorganisms and pathogens. The intestinal flora can also affect the immune response through metabolites such as short-chain fatty acids (SCFAs). SCFAs (such as acetate, propionate, and butyrate) can regulate the activity of immune cells, reduce inflammation, and promote an anti-inflammatory environment. The intestinal flora helps maintain the integrity of the intestinal barrier, preventing pathogens and harmful substances from entering the body through intestinal epithelial cells, thereby reducing inflammation and autoimmune responses. The composition of the intestinal flora is also related to resistance to various diseases, such as cancer. Certain intestinal bacteria such as Bacteroides fragilis may enhance the body's immunity to cancer through interactions with vitamin D. The intestinal flora is affected by factors such as diet, environment, and host, and our daily diet is the key to determining the structure and function of the intestinal flora community. At present, a large number of research results have shown that regulating the intestinal flora through diet, thereby regulating the physiological function of the host, is a recognized convenient and efficient nutritional intervention strategy.

[0004] Microbiota-directed food (MDF) is a product designed to change the intestinal microbial structure of consumers. Compared with traditional foods, MDF has a very clear goal. It mainly targets specific intestinal flora and provides substrates that can be converted by intestinal microbiota, thereby accurately and directedly regulating the intestinal flora, specifically changing the composition and structure of one or some intestinal microorganisms, affecting their functional characteristics and metabolites, and thus affecting the health of the body. At the 2024 International Food Safety and Health Conference, microbiota-targeted food was listed as one of the top ten research hotspots in global food safety and health. Existing microbiota-targeted foods that improve human immunity cannot meet market needs. Summary of the invention

[0005] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a targeted formula food for intestinal flora that improves immunity. As a special food, the purpose is to improve human immunity and enhance human physique; the second purpose is to provide a method for preparing a targeted formula food for intestinal flora that improves immunity.

[0006] In order to solve the above technical problems, the present invention adopts the following technical solutions: A targeted formula food for intestinal flora for improving immunity, characterized in that its components and their mass ratios are: 75-85 parts of raw milk, 1-5 parts of white sugar, 5-10 parts of inulin, 5-10 parts of oats, 5-10 parts of resistant dextrin, and 3-8 parts of xylooligosaccharides; the fermentation bacteria are added in an amount of 0.3-1.0% of the mass of the raw milk.

[0007] Furthermore: the fermentation bacteria is kefir bacteria.

[0008] Furthermore, the oats are oats that have been cooked or steamed to reach an edible state.

[0009] Furthermore, the inulin is natural inulin derived from chicory, with a degree of polymerization of 2-60.

[0010] Furthermore, the oat ripening treatment method is as follows: the oats are cleaned, water required by the process is added for cooking, and gelatinized at 100°C for 15-40 minutes, and then the gelatinized oat grains are fed into a double-roller sheet press to form uniform flakes, and then dried on a fluidized bed to reduce the moisture content to less than 11%, cooled with cold air, and finally sieved through a cave to remove lumps and fine particles to obtain ripe oat flakes.

[0011] A method for preparing an intestinal flora targeted formula food for improving immunity, characterized by comprising the following steps: 1) Preparing and adding auxiliary materials: preparing the components according to the process requirements; adding inulin, resistant dextrin, xylo-oligosaccharide and white sugar to the pretreated raw milk according to the product formula ratio, and mixing them thoroughly to obtain a raw milk mixture; 2) Treatment of raw milk mixture: The raw milk mixture is sterilized and disinfected; 3) Inoculation of bacteria: Under sterile conditions, inoculate the bacteria into the raw milk mixture according to the proportion required by the process, and mix so that the bacteria are evenly distributed; 4) Fermentation: The raw milk mixture inoculated with the bacteria is placed in a constant temperature environment for fermentation; the fermentation time is 12-24 hours, and the fermentation temperature is maintained at 24-28°C; 5) Add the cooked oats: When the fermentation is almost complete, add the pre-cooked oats and stir gently; 6) Product refrigeration: After the fermentation time is reached, the fermented product is refrigerated to stop the fermentation activity; 7) Product filling and storage: Fill the product into a clean container under sterile conditions and seal it to ensure it is airtight; store the filled product under refrigerated conditions.

[0012] Furthermore, in step 1), the fresh raw milk is pretreated, including acceptance and filtration; the acceptance criteria include sensory indicators, physical and chemical indicators and microbiological indicators; the sensory indicators include color, smell and state; the physical and chemical indicators include relative density, acidity, freezing point detection and alcohol test; the microbial indicators include bacterial overview and antibiotic residues.

[0013] Furthermore, in step 6), after the fermentation time is reached, the mixture is stored at 4°C for several hours to one day for post-fermentation treatment.

[0014] Furthermore, in step 1), the prepared white sugar, inulin, resistant dextrin and xylo-oligosaccharide are added to the raw milk in proportion and mixed and stirred, and then a secondary homogenization treatment is performed. The homogenization conditions are: the pressure is set between 150-200 bar and the temperature is between 50-65°C.

[0015] A method for consuming an intestinal flora targeted formula food for improving immunity. The food prepared by the method of the invention has an edible amount of 2 cups per day, between 700 and 1000 ml.

[0016] The intestinal flora targeted formula food for improving immunity and the preparation method thereof of the present invention have the following beneficial effects compared with the prior art: 1. The intestinal flora targeted formula food for improving immunity of the present invention is rich in dietary fiber, and prebiotics such as inulin and resistant dextrin are added with probiotics, which can promote intestinal peristalsis, help maintain the balance of intestinal microorganisms, regulate the structure and diversity of intestinal flora, increase the expression of sugar-degrading carbohydrate active enzymes encoded by microorganisms, promote the production of short-chain fatty acids, reduce inflammatory signals and vitality, and improve the body's immunity.

[0017] 2. The intestinal flora targeted formula food for improving immunity of the present invention has low cost and is suitable for long-term consumption by a wide range of patients with decreased resistance and low immunity.

[0018] 3. The preparation method of the present invention is simple and applicable, and is suitable for the needs of industrial production. DETAILED DESCRIPTION

[0019] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] The present invention discloses an intestinal flora targeted formula food for improving immunity, the components and mass ratio of which are: 75-85 parts of raw milk, 1-5 parts of white sugar, 5-10 parts of inulin, 5-10 parts of oats, 5-10 parts of resistant dextrin, and 3-8 parts of oligoxylose; the fermentation strains used can be selected as applicable, such as Kefir strains, and the addition amount is 0.3-1.0% of the mass of raw milk. The oats are instant oats, which are oats that have been cooked, steamed, or otherwise cooked to reach an edible state.

[0021] The inulin, resistant dextrin, xylooligosaccharide and white sugar are all commercially available brand products.

[0022] As a further improvement of the present invention, the inulin is natural inulin derived from chicory, with a degree of polymerization of 2-60.

[0023] The method for preparing the intestinal flora targeted formula food for improving immunity of the present invention comprises the following steps: 1) Preparation and addition of auxiliary materials: prepare the aforementioned components according to the process requirements; add inulin, resistant dextrin, xylo-oligosaccharide and white sugar to the pretreated raw milk according to the product formula ratio, mix well and evenly to obtain a raw milk mixture; 2) Treatment of the raw milk mixture: The raw milk mixture is sterilized and disinfected; usually pasteurization is performed, usually by heating at 72-75°C for 15-20 seconds and then rapidly cooling to the inoculation temperature (usually 24-28°C); 3) Inoculation of bacteria (kefir bacteria): Under sterile conditions, inoculate the bacteria into the raw milk mixture according to the proportion required by the process, ensuring that it is fully mixed so that the bacteria are evenly distributed; 4) Fermentation: The inoculated raw milk mixture is placed in a constant temperature environment for fermentation; the fermentation time is carried out according to the process requirements, usually the fermentation time is 12-24 hours, and the fermentation temperature is maintained at 24-28°C. The fermentation time or temperature is usually adjusted according to the required acidity and taste; during the fermentation process, lactic acid bacteria consume lactose to produce lactic acid, making the product acidic.

[0024] 5) Add the cooked oats: When the fermentation is almost complete, add the pre-cooked oats and stir gently; The oat ripening treatment is as follows: the oats are cleaned and a proper amount of water is added for ripening, such as cooking or steaming; the excess water is removed from the treated oats by filtering or centrifuging to achieve the degree of maturity and water content required by the process, and the oats are set aside; 6) Product refrigeration: After the fermentation time is reached, the fermented product is refrigerated to stop the fermentation activity and keep the product fresh and stable.

[0025] Furthermore, after the fermentation time is reached, a post-fermentation treatment can be selected to enhance the flavor as needed, usually by storing the mixture at a relatively low temperature (such as 4° C.) for several hours to a day for post-fermentation treatment.

[0026] 7) Product filling and storage: Fill the product into clean containers under sterile conditions and seal them to ensure airtightness. Store the filled product under refrigerated conditions to ensure stable product quality.

[0027] In step 1), fresh raw milk is pre-treated, including acceptance, filtration, etc.; Among them, the acceptance criteria include sensory indicators, physical and chemical indicators and microbiological indicators; sensory indicators include color, smell and state; physical and chemical indicators include relative density, acidity, freezing point test and alcohol test; microbiological indicators include bacterial overview and antibiotic residues. Other indicators such as protein content should not be less than 2.9%, fat content should not be less than 3.1%, non-fat milk solids content should not be less than 8.1%, and pollutant limits should comply with the corresponding national food safety standards.

[0028] In step 5), the oatmeal is cooked as follows: the oats (imported from Australia) are cleaned, an appropriate amount of water (in accordance with the process requirements) is added for cooking, and the oats are treated at 100°C for 15-40 minutes for gelatinization, and then the gelatinized oatmeal grains are fed into a double-roller sheet press to form uniform flakes, and then dried on a fluidized bed to reduce the moisture content to less than 11%, cooled with cold air, and finally graded through a kiln sieve to remove lumps and fine particles to obtain cooked oatmeal for later use.

[0029] Step 1) also includes mixing and homogenizing the ingredients; the prepared white sugar, inulin, resistant dextrin, and oligoxylose are added to the raw milk in proportion and mixed and stirred, and then a second homogenization is performed. The homogenization conditions are: the pressure is set between 150-200 bar and the temperature is between 50-65℃. Milk homogenization is to break the fat particles in the milk into smaller particles through high pressure to prevent the fat from floating up and causing stratification.

[0030] In steps 3) and 4), the specific operations of inoculation and fermentation are: inoculate the kefir strains into the milk mixture at the recommended ratio under sterile conditions, ensure sufficient mixing so that the strains are evenly distributed; place the inoculated milk mixture in a constant temperature environment for fermentation; the fermentation time is adjusted according to the required acidity and taste, usually the fermentation time is 12-24 hours, and the fermentation temperature is maintained at 24-28°C. During the fermentation process, lactic acid bacteria consume lactose to produce lactic acid, acidifying the product.

[0031] The intestinal flora targeted formula food for improving immunity of the present invention is ready to eat, and the recommended consumption is 2 cups per day, about 700-1000 ml.

[0032] Embodiment 1 The intestinal flora targeted formula food for improving immunity prepared by the present invention comprises the following raw materials by weight: 75 parts of raw milk, 2 parts of white sugar, 5 parts of inulin, 8 parts of instant oats, 5 parts of resistant dextrin, and 5 parts of oligoxylose. The amount of kefir bacteria added is 0.5% of the mass of raw milk. The food is prepared according to the above-mentioned preparation method.

[0033] Embodiment 2 The intestinal flora targeted formula food for improving immunity prepared by the present invention comprises the following raw materials by weight: 80 parts of raw milk, 1 part of white sugar, 5 parts of inulin, 5 parts of instant oats, 6 parts of resistant dextrin, and 4 parts of oligoxylose. The amount of kefir bacteria added is 0.6% of the mass of raw milk. The food is prepared according to the above-mentioned preparation method.

[0034] Embodiment 3 The intestinal flora targeted formula food for improving immunity prepared by the present invention comprises the following raw materials by weight: 85 parts of raw milk, 5 parts of white sugar, 10 parts of inulin, 10 parts of instant oats, 10 parts of resistant dextrin, and 8 parts of oligoxylose. The amount of kefir bacteria added is 0.8% of the mass of raw milk. The food is prepared according to the above-mentioned preparation method.

[0035] Embodiment 4 The intestinal flora targeted formula food for improving immunity prepared by the present invention comprises the following raw materials by weight: 85 parts of raw milk, 5 parts of white sugar, 2 parts of inulin, 5 parts of instant oats, 2 parts of resistant dextrin, and 1 part of xylo-oligosaccharide. The amount of kefir bacteria added is 0.5% of the mass of raw milk. The food is prepared according to the above-mentioned preparation method.

[0036] Animal experiment: Detect whether the intestinal flora targeted formula food prepared by the present invention has the effect of improving immunity.

[0037] Experimental process: 40 male dextran sulfate sodium (DSS)-induced enteritis C57BL / 6 mice aged 8 weeks were divided into 4 groups, 10 mice in each group, a control group and 3 experimental groups; the mice were given DSS solution by free drinking for 28 days to establish a chronic inflammation mouse model. Then the mice were gavaged with formula fermented yogurt, 2 ml / mouse per day; the control group had a normal diet; the test was performed after 30 days of feeding. The test results are as follows: index IL-10 IL-6 <![CDATA[CD4 + T cells]]> <![CDATA[Foxp3 + ]]> Myosin-1 Experimental Group 1 + + + Experimental Group 2 + + + + Experimental Group 3 + + + + + Note: Experimental groups 1, 2, and 3 correspond to the formula foods in Examples 1, 2, and 3 above, respectively. Columns with "+" in the table indicate a significant increase in expression abundance relative to the control group, and blanks without "+" indicate no significant change.

[0038] The experimental results show that after consuming the formula food prepared by the present invention, the expression of related factors and proteins in inflammatory mice increased significantly, showing a good anti-inflammatory effect and a more obvious effect of improving immunity.

[0039] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the technical solution. Those skilled in the art should understand that those modifications or equivalent substitutions of the technical solution of the present invention that do not depart from the purpose and scope of the technical solution should be included in the scope of the claims of the present invention.

Claims

1. A formula food targeting intestinal flora for improving immunity, characterized in that: Its components and their mass ratio are: 75-85 parts of raw milk, 1-5 parts of white sugar, 5-10 parts of inulin, 5-10 parts of oats, 5-10 parts of resistant dextrin, and 3-8 parts of xylooligosaccharide; the fermentation bacteria are added in an amount of 0.3-1.0% of the mass of the raw milk.

2. The intestinal flora targeted formula food for improving immunity according to claim 1, characterized in that: The fermentation bacteria is kefir bacteria.

3. The intestinal flora targeted formula food for improving immunity according to claim 1, characterized in that: The oats are oats that are cooked, steamed, and cooked to be edible.

4. The intestinal flora targeted formula food for improving immunity according to claim 1, characterized in that: The inulin is natural inulin derived from chicory, and has a degree of polymerization of 2-60.

5. The intestinal flora targeted formula food for improving immunity according to claim 2, characterized in that: The oat ripening treatment method is as follows: the oats are cleaned, water required by the process is added for cooking, and gelatinized at 100° C. for 15-40 minutes, and then the gelatinized oat grains are fed into a double-roller sheet press to form uniform flakes, and then dried on a fluidized bed to reduce the moisture content to less than 11%, cooled with cold air, and finally sieved through a cave to remove lumps and fine particles to obtain ripe oat flakes.

6. A method for preparing an intestinal flora targeted formula food for improving immunity, characterized in that The steps include: 1) Preparing and adding auxiliary materials: preparing the components according to the process requirements; adding inulin, resistant dextrin, xylo-oligosaccharide and white sugar to the pretreated raw milk according to the product formula ratio, and mixing them thoroughly to obtain a raw milk mixture; 2) Treatment of raw milk mixture: The raw milk mixture is sterilized and disinfected; 3) Inoculation of bacteria: Under sterile conditions, inoculate the bacteria into the raw milk mixture according to the proportion required by the process, and mix so that the bacteria are evenly distributed; 4) Fermentation: The raw milk mixture inoculated with the bacteria is placed in a constant temperature environment for fermentation; the fermentation time is 12-24 hours, and the fermentation temperature is maintained at 24-28°C; 5) Add the cooked oats: When the fermentation is almost complete, add the pre-cooked oats and stir gently; 6) Product refrigeration: After the fermentation time is reached, the fermented product is refrigerated to stop the fermentation activity; 7) Product filling and storage: Fill the product into a clean container under sterile conditions and seal it to ensure it is airtight; store the filled product under refrigerated conditions.

7. The method for preparing the intestinal flora targeted formula food for improving immunity according to claim 6, characterized in that: In step 1), fresh raw milk is pretreated, including acceptance and filtration; the acceptance criteria include sensory indicators, physical and chemical indicators and microbiological indicators; the sensory indicators include color, smell and state; the physical and chemical indicators include relative density, acidity, freezing point detection and alcohol test; the microbiological indicators include bacterial overview and antibiotic residues.

8. The method for preparing the intestinal flora targeted formula food for improving immunity according to claim 6, characterized in that: In step 6), after the fermentation time is reached, the mixture is stored at 4°C for several hours to one day for post-fermentation.

9. The method for preparing the intestinal flora targeted formula food for improving immunity according to claim 6, characterized in that: In step 1), the prepared white sugar, inulin, resistant dextrin and oligoxylose are added to the raw milk in proportion and mixed and stirred, and then a secondary homogenization treatment is performed. The homogenization conditions are: the pressure is set between 150-200 bar and the temperature is between 50-65°C.

10. A method for consuming a formula food targeting intestinal flora for improving immunity, characterized in that: The food prepared by the method according to any one of claims 6 to 9 has a consumption of 2 cups per day, between 700-1000 ml.