A probiotic composition with weight loss effect and preparation method thereof

Abstract

The invention discloses a probiotic composition with a weight-loss effect and a preparation method thereof, and relates to the technical field of probiotics; the probiotic composition with a weight-loss effect consists of a modified celery IDF multifunctional carrier, a fat-reducing gel protective material, a traditional Chinese medicine prebiotic complex and plant lactobacillus; the fat-reducing gel protective material consists of sodium alginate, pectin, citric acid, lecithin and calcium chloride; the traditional Chinese medicine prebiotic complex consists of ginseng, wolfberry, mulberry polyphenols and inulin; plant lactobacillus is enriched on the modified celery IDF multifunctional carrier for fermentation and culture, the obtained composite culture is synergistically matched with the traditional Chinese medicine prebiotic complex, and under the wrapping of the fat-reducing gel protective material, the activity of the probiotics is effectively improved, the tolerance to gastrointestinal digestive juice is enhanced, it is beneficial to the absorption and utilization of more probiotics and their metabolites in the colon, and the weight-loss effect is maximized.

Description

Technical Field

[0001] The invention belongs to the technical field of probiotics, and specifically refers to a probiotic composition with weight loss effect and a preparation method thereof. Background Art

[0002] The root cause of obesity is the accumulation of too much fat in the body, accompanied by a low-level systemic inflammatory response and bad behavioral patterns (including dietary trends, physical exercise, etc.), which results in an imbalance between energy input and consumption. The "source" of obesity is people's preference for high-fat and high-calorie foods, which leads to overnutrition, changes in human lipid metabolism, and causes disorders in the intestinal microbiome. Therefore, improving intestinal microorganisms through microbial intervention to reduce obesity and related metabolic diseases may become an important intervention method for obese people.

[0003] Gut microorganisms affect the "brain-gut" axis through intestinal hormones and vagus nerve connections (which affect energy consumption and areas in the central nervous system related to eating behavior), thereby changing host behavior. At the same time, intestinal microbial metabolites and their products can also act as signal molecules and regulate the hormone secretion of enteroendocrine cells, thereby regulating appetite, intestinal motility, energy absorption, storage, and energy consumption and other feeding-related behaviors, thereby affecting the development of obesity. Compared with traditional drugs, probiotics have lower side effects, higher safety and stability, and can reduce obesity to a certain extent, thereby achieving preventive and therapeutic effects.

[0004] The existing technology currently has the following problems:

[0005] Probiotics are easily affected by the external environment during storage, resulting in lower activity. After entering the digestive tract, they have poor tolerance to digestive juices, which further reduces the survival rate of the bacteria and limits the weight loss effect. Summary of the invention

[0006] In view of the above situation, in order to overcome the defects of the prior art, the present invention proposes a probiotic composition with weight loss effect, comprising the following components in parts by weight: 20-30 parts of modified celery IDF multifunctional carrier, 10-20 parts of fat-reducing gel protective material, 8-12 parts of traditional Chinese medicine prebiotic complex, and 1-2 parts of plant lactobacillus.

[0007] The modified celery IDF multifunctional carrier is a structural material obtained by jointly modifying celery insoluble fiber with subcritical water and alkaline hydrogen peroxide, and is used as a fermentation carrier of Lactobacillus plantarum.

[0008] The fat-reducing gel protective material comprises the following components in parts by weight: 15-18 parts of sodium alginate, 12-15 parts of pectin, 3-6 parts of citric acid, 6-9 parts of lecithin, and 6-9 parts of calcium chloride.

[0009] The traditional Chinese medicine prebiotic complex comprises the following components in parts by weight: 3-5 parts of ginseng, 3-5 parts of wolfberry, 1-2 parts of mulberry polyphenols, and 1-2 parts of inulin.

[0010] The preparation method of the modified celery IDF multifunctional carrier specifically comprises the following steps:

[0011] (1) washing and cutting celery, beating the celery in a material-water ratio of 1:1, passing the slurry through a 100-200 mesh screen, removing celery juice, and collecting celery residue. The celery residue mainly includes cellulose, hemicellulose, lignin, chitosan, etc. The structure of the water-insoluble fiber has a large number of dense vascular bundles, which are filamentous, rough on the surface, and large specific surface area, which is conducive to the attachment of probiotic bacteria. The celery water-insoluble dietary fiber also has the effects of increasing satiety, helping to lose weight, and reducing edema, thereby obtaining celery IDF;

[0012] (2) 8 g of the celery IDF described in step (1) is added to 80-100 mL of distilled water, stirred and mixed, and then placed in a multifunctional stirrer of a reactor, and treated at 180-220° C. for 10-30 min. After the treatment, the reactor is immediately cooled to room temperature with tap water, and the solid residue is collected using a vacuum pump, washed with distilled water until neutral, dried, and ground. Subcritical water modification does not require the addition of additional chemical reagents, so it has the advantages of a clean reaction process and high extraction efficiency. The high temperature degrades the cell wall polysaccharide, so that the celery IDF has a loose porous structure and exposes the adsorption site, thereby obtaining subcritical water modified celery IDF;

[0013] (3) 10 g of the subcritical water-modified celery IDF described in step (2) was added to 200 mL of hydrogen peroxide solution. A network structure appeared in the celery IDF after subcritical water modification, which reduced the interaction between cellulose, hemicellulose and lignin, and was more conducive to the entry of hydrogen peroxide into the interior to play a role, thereby facilitating a more thorough destruction of the connecting bonds between hemicellulose and lignin. The pH was adjusted to 11.0 with a 5 mol / L sodium hydroxide solution, and then placed in a constant temperature water bath and stirred for 3-4 h, the temperature was controlled at 70-80° C., and then the pH was adjusted to neutral with a 1 mol / L hydrochloric acid solution. After the end, a vacuum pump was used to collect the solid residue, which was washed with distilled water 2-3 times, dried, and ground. The combined modification treatment of subcritical water and hydrogen peroxide exposed a large amount of cellulose, thereby providing the celery IDF with more adsorption sites, opening the internal structure, facilitating the attachment of probiotics and playing a protective role, and further promoting the proliferation of probiotics, thereby obtaining a modified celery IDF multifunctional carrier;

[0014] Preferably, in step (3), the mass fraction of the hydrogen peroxide solution is 1.0-2.0%. The addition of hydrogen peroxide can degrade the biomass connecting bonds in the lignin, expose the internal structure and active groups, and enhance the adsorption capacity.

[0015] The preparation method of the traditional Chinese medicine prebiotics complex specifically comprises the following steps:

[0016] a. Add water to ginseng and wolfberry and soak them in a decoction machine for 2-4 hours. Soaking is beneficial to the leaching of effective ingredients of traditional Chinese medicine and improves the extraction efficiency. Decoction is performed 1-2 times, and the extraction is performed for 2-3 hours with slight boiling. The decoction is combined, and the filter residue is discarded. The collected filtrate is concentrated, and the vacuum pressure is -0.06MPa and the temperature is 40-50°C. The filtrate is dried and crushed. Ginseng and wolfberry contain active ingredients such as saponins and polysaccharides, which have significant prebiotic properties and can promote the growth of intestinal bacteria such as Lactobacillus and Bifidobacterium to obtain a traditional Chinese medicine extract;

[0017] b. Centrifuge the mulberry juice at 4000 rpm for 10 min at 4°C, slowly pour the supernatant into the chromatography column loaded with X-5 macroporous resin, and after the macroporous resin is saturated with adsorption, wash it with distilled water to remove sugar, acid and other water-soluble impurities, then elute it with acidic ethanol solution, collect the eluate, and decompress and concentrate it in a rotary evaporator until all the ethanol is recovered, control the concentration temperature at 38-42°C, and freeze-dry. Mulberry polyphenols have prebiotic properties, can regulate intestinal microecology, have the effect of promoting beneficial intestinal bacteria, and play a synergistic function through interaction with intestinal microorganisms. Mulberry polyphenols can also regulate the body's lipid metabolism, affect the proliferation, differentiation and apoptosis of fat cells, stimulate the body's heat production and accelerate energy consumption, thereby obtaining mulberry polyphenols;

[0018] c. Disperse 1-2 g of inulin in 10 mL of pure water, stir magnetically for 1-2 h, then add 1-2 g of the mulberry polyphenols described in step b and 3-5 g of the Chinese herbal extract described in step a. The combination of mulberry polyphenols, inulin and Chinese herbal extract can protect polyphenols from degradation and improve stability. Mulberry polyphenols and inulin also give the Chinese herbal extract multiple prebiotic effects. Continue magnetic stirring for 3-4 h, freeze-dry, and obtain a Chinese herbal prebiotic complex.

[0019] Preferably, in step b, in the acidic ethanol solution, the volume ratio of anhydrous ethanol, water and hydrochloric acid solution is 80:19:1, and the acidic ethanol solution can fully elute the phenolic substances in the macroporous resin.

[0020] The present invention also provides a method for preparing a probiotic composition having a weight loss effect, which specifically comprises the following steps:

[0021] S1. Inoculate 1-2g of plant lactobacillus into a blue-mouth bottle containing MRS culture medium and modified celery IDF multifunctional carrier and culture for 36h. The modified celery IDF multifunctional carrier can effectively enrich probiotics, has a good protective effect on the activity of probiotics, and reduces the adverse effects of the external environment on the activity of probiotics. Filter and remove the supernatant. Put the modified celery IDF multifunctional carrier containing plant lactobacillus into a fermentation tank, add MRS culture medium to reach 80% of the tank volume, and culture at 37°C for 7d. Replace the MRS culture medium in the tank every 1d. After the cultivation is completed, discard the supernatant and freeze-dry. At this time, the content of plant lactobacillus is 13-14LogCFU / g. Utilize the principle of biofilm enrichment of microorganisms to ferment and culture the modified celery IDF multifunctional carrier and probiotics together. Not only the probiotic effect of the culture is enhanced, but also the tolerance to gastrointestinal digestive juice is effectively improved, which is conducive to better maintaining the activity of probiotics, thereby achieving an ideal weight loss effect and obtaining a composite culture.

[0022] S2, add lecithin and calcium chloride to 50mL water, wherein the mass fraction of calcium chloride is 4-6%, stir magnetically to fully dissolve it, and set aside, then add 5-6g sodium alginate and 4-5g pectin to 80-100mL water, stir to completely hydrate it, add 1-2g citric acid for cross-linking, adjust the pH to 5.0-6.0, the cross-linking temperature to 40-50°C, and the cross-linking time to 30-50min. The cross-linking of citric acid enhances the stability of sodium alginate and pectin, which is beneficial to enhance the mechanical properties of the fat-reducing gel protective material and further enhances the coating of probiotics. Cool to room temperature, then add the composite culture and the Chinese medicine prebiotics complex described in step S1, stir evenly, drip into the calcium chloride lecithin solution at a speed of 1 mL / min using a syringe automatic propulsion pump, let stand for 5-10 minutes to solidify, filter with a 200-mesh gauze filter, freeze-dry the precipitate, and the fat-reducing gel protective material wraps the composite culture and the Chinese medicine prebiotics complex, further enhancing the protective effect on the probiotics, and can be released at a fixed point in the colon, reducing the damage of the gastrointestinal tract to the probiotics, thereby also improving the weight-loss effect of the probiotics, and obtaining a probiotic composition with a weight-loss effect;

[0023] Preferably, in step S2, the amount of lecithin added is 2-3 g. Lecithin helps to promote the uniform distribution between polysaccharide molecular chains, fill the coagulation structure of the fat-reducing gel protective material, improve stability and protection, and lecithin also has the function of decomposing emulsified oils.

[0024] The beneficial effects achieved by the present invention are as follows:

[0025] The present invention enriches plant lactobacillus and ferments and cultures it on a modified celery IDF multifunctional carrier, and the obtained composite culture is synergistically matched with a traditional Chinese medicine prebiotic complex. Moreover, under the wrapping of a fat-reducing gel protective material, the activity of the probiotics is effectively improved, and the tolerance to gastrointestinal digestive juice is enhanced, which is conducive to the absorption and utilization of more probiotics and their metabolites in the colon, thereby maximizing the weight-loss effect. In the modified celery IDF multifunctional carrier, a large amount of fiber of the celery IDF is modified by dual combined modification of subcritical water and hydrogen peroxide, The probiotics are exposed and have more adsorption sites, which is conducive to the attachment and enrichment of probiotics on the surface or in the cavity of the carrier, reducing the adverse effects of the external environment on the activity of probiotics, and celery IDF is not decomposed by digestive juices and enzymes, and has a certain digestibility, which can reduce the digestion and decomposition of probiotics after entering the gastrointestinal tract, thereby protecting the activity and number of probiotics; in the traditional Chinese medicine prebiotics complex, the traditional Chinese medicine prebiotics are combined with mulberry polyphenols and inulin, which gives multiple prebiotic effects, enhances antioxidant properties, and has a positive effect on the activity of probiotics. The invention discloses a kind of probiotic composition with weight loss effect. The invention discloses a probiotic composition with weight loss effect. The invention discloses a probiotic composition with weight loss effect. The invention discloses a probiotic composition with weight loss effect. The invention discloses a probiotic composition with weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. The probiotic composition has weight loss effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a scanning electron microscope image of the modified celery IDF multifunctional carrier prepared in Example 1 of the present invention;

[0027] Figure 2 This is a scanning electron microscope image of the probiotic composition with weight loss effect prepared in Example 1 of the present invention;

[0028] Figure 3 The results of the viable bacterial counts during the storage period of Examples 1-4 and Comparative Examples 1-3 of the present invention are shown in FIG.

[0029] Figure 4The figure is a result diagram of the number of viable bacteria after simulated gastrointestinal digestion of Examples 1-4 of the present invention and Comparative Examples 1-3;

[0030] Figure 5 The figure is a graph showing the weight results of mice in Examples 1-4 of the present invention and Comparative Examples 1-3. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0032] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein may be applied to the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only and are not intended to limit the content of this application.

[0033] The experimental methods in the following examples are conventional methods unless otherwise specified; the experimental materials used in the following examples are purchased from commercial channels unless otherwise specified.

[0034] The sources of the reagents used in the examples are as follows:

[0035] Lactobacillus plantarum brand Bncc, item number BNCC339790;

[0036] Sodium alginate CAS No: 9005-38-3, brand Innochem, product number A02570;

[0037] Pectin CAS No: 9000-69-5, brand Tci, item number P0024;

[0038] Citric acid CAS No: 77-92-9, brand Innochem, product number A24418;

[0039] Lecithin CAS No: 8002-43-5, brand Innochem, product number A71764;

[0040] Calcium chloride CAS No: 10043-52-4, brand Innochem, product number A06389;

[0041] Inulin CAS No: 9005-80-5, brand Apinno, product number GC79039;

[0042] Hydrochloric acid CAS No: 7647-01-0, brand Innochem, product number A04558;

[0043] Hydrogen peroxide CAS No: 7722-84-1, brand Acros, product number 202460010;

[0044] MRS culture medium brand Macklin, product number M775817-250g;

[0045] Sodium hydroxide CAS No: 1310-73-2, brand Innochem, product number A36865;

[0046] Anhydrous ethanol CAS No: 64-17-5, brand Innochem, product number G00004.

[0047] Example 1

[0048] This embodiment proposes a probiotic composition with a weight loss effect, comprising the following components in parts by weight: 30 parts of modified celery IDF multifunctional carrier, 20 parts of fat-reducing gel protective material, 12 parts of traditional Chinese medicine prebiotic complex, and 2 parts of plant lactobacillus.

[0049] The modified celery IDF multifunctional carrier is a structural material obtained by jointly modifying celery insoluble fiber with subcritical water and alkaline hydrogen peroxide, and is used as a fermentation carrier of Lactobacillus plantarum.

[0050] The fat-reducing gel protective material comprises the following components in parts by weight: 18 parts of sodium alginate, 15 parts of pectin, 6 parts of citric acid, 9 parts of lecithin and 9 parts of calcium chloride.

[0051] The traditional Chinese medicine prebiotic complex comprises the following components in parts by weight: 5 parts of ginseng, 5 parts of wolfberry, 2 parts of mulberry polyphenols and 2 parts of inulin.

[0052] The preparation method of the modified celery IDF multifunctional carrier specifically comprises the following steps:

[0053] (1) washing and cutting celery, beating the celery with a material-water ratio of 1:1, passing the slurry through a 200-mesh sieve, removing celery juice, and collecting celery residue. The celery residue mainly includes cellulose, hemicellulose, lignin, chitosan, etc. The structure of the water-insoluble fiber has a large number of dense vascular bundles, which are filamentous, rough on the surface, and large specific surface area, which is conducive to the attachment of probiotic bacteria. The celery water-insoluble dietary fiber also has the effects of increasing satiety, helping to lose weight, and reducing edema, thereby obtaining celery IDF;

[0054] (2) 8 g of the celery IDF described in step (1) was added to 100 mL of distilled water, stirred and mixed, and then placed in a multifunctional stirrer of a reactor, and treated at 220° C. for 30 min. After the treatment, the reactor was immediately cooled to room temperature with tap water, and solid residues were collected using a vacuum pump, washed with distilled water until neutral, dried, and ground. Subcritical water modification does not require the addition of additional chemical reagents, so it has the advantages of a clean reaction process and high extraction efficiency. High temperature degrades cell wall polysaccharides, so that the celery IDF has a loose porous structure and exposes adsorption sites, thereby obtaining subcritical water-modified celery IDF;

[0055] (3) 10 g of the subcritical water-modified celery IDF described in step (2) was added to 200 mL of hydrogen peroxide solution, the mass fraction of the hydrogen peroxide solution being 2.0%. The addition of hydrogen peroxide can degrade the biomass connecting bonds in lignin, expose the internal structure and active groups, and enhance the adsorption capacity. A network structure appeared in the celery IDF after subcritical water modification, which reduced the interaction between cellulose, hemicellulose and lignin, and was more conducive to the entry of hydrogen peroxide into the interior to play a role, thereby facilitating a more thorough destruction of the connecting bonds between hemicellulose and lignin. 5 mo l / L sodium hydroxide solution was used to adjust the pH to 11.0, and then placed in a constant temperature water bath and stirred for 4 hours, the temperature was controlled at 80℃, and then the pH was adjusted to neutral with 1mol / L hydrochloric acid solution. After the end, a vacuum pump was used to collect the solid residue, washed with distilled water 3 times, dried, ground, and modified by subcritical water and hydrogen peroxide. A large amount of cellulose was exposed, so that the celery IDF had more adsorption sites and the internal structure was opened, which was conducive to the attachment of probiotics and played a protective role, further promoting the proliferation of probiotics, and obtaining a modified celery IDF multifunctional carrier.

[0056] The preparation method of the traditional Chinese medicine prebiotic complex specifically comprises the following steps:

[0057] a. Add water to ginseng and wolfberry and soak them in a decoction machine for 4 hours. Soaking is beneficial to the leaching of effective ingredients of traditional Chinese medicine and improves the extraction efficiency. Decoction is performed twice, and the extraction is performed for 3 hours with slight boiling. The decoction is combined, and the filter residue is discarded. The collected filtrate is concentrated, and the vacuum pressure is -0.06MPa and the temperature is 50°C. The filtrate is dried and crushed. Ginseng and wolfberry contain active ingredients such as saponins and polysaccharides, which have significant prebiotic properties and can promote the growth of intestinal bacteria such as Lactobacillus and Bifidobacterium to obtain a traditional Chinese medicine extract;

[0058] b. Centrifuge the mulberry juice at 4000 rpm for 10 min at 4°C, slowly pour the supernatant into the chromatography column loaded with X-5 macroporous resin, and after the macroporous resin is saturated with adsorption, wash it with distilled water to remove sugar, acid and other water-soluble impurities, and then elute it with an acidic ethanol solution. In the acidic ethanol solution, the volume ratio of anhydrous ethanol, water and hydrochloric acid solution is 80:19:1. The acidic ethanol solution can fully elute the phenolic substances in the macroporous resin. Collect the eluate, and concentrate it under reduced pressure in a rotary evaporator until all ethanol is recovered. Control the concentration temperature at 42°C and freeze-dry. Mulberry polyphenols have prebiotic properties, can regulate intestinal microecology, have the effect of promoting beneficial intestinal bacteria, and play a synergistic function through interaction with intestinal microorganisms. Mulberry polyphenols can also regulate the body's lipid metabolism, affect the proliferation, differentiation and apoptosis of fat cells, stimulate the body's heat production and accelerate energy consumption, and obtain mulberry polyphenols;

[0059] c. Disperse 2 g of inulin in 10 mL of pure water and stir magnetically for 2 h. Then add 2 g of the mulberry polyphenols described in step b and 5 g of the traditional Chinese medicine extract described in step a. The combination of mulberry polyphenols, inulin and traditional Chinese medicine extract can protect polyphenols from degradation and improve stability. Mulberry polyphenols and inulin also give the traditional Chinese medicine extract multiple prebiotic effects. Continue magnetic stirring for 4 h and freeze-dry to obtain a traditional Chinese medicine prebiotic complex.

[0060] This embodiment provides a method for preparing a probiotic composition having a weight loss effect, which specifically comprises the following steps:

[0061] S1. Inoculate 2g of plant lactobacillus into a blue-mouth bottle containing MRS culture medium and modified celery IDF multifunctional carrier and culture for 36h. The modified celery IDF multifunctional carrier can effectively enrich probiotics, has a good protective effect on the activity of probiotics, and reduces the adverse effects of the external environment on the activity of probiotics. Filter and remove the supernatant. Put the modified celery IDF multifunctional carrier containing plant lactobacillus into a fermentation tank, add MRS culture medium to reach 80% of the tank volume, and culture at 37°C for 7d. Replace the MRS culture medium in the tank every 1d. After the cultivation is completed, discard the supernatant and freeze-dry. At this time, the content of plant lactobacillus is 14LogCFU / g. Utilize the principle of biofilm enrichment of microorganisms to ferment and culture the modified celery IDF multifunctional carrier and probiotics together. Not only the probiotic effect of the culture is enhanced, but also the tolerance to gastrointestinal digestive juice is effectively improved, which is conducive to better maintaining the activity of probiotics, thereby achieving an ideal weight loss effect and obtaining a composite culture.

[0062] S2, lecithin and calcium chloride are added to 50mL water, wherein the mass fraction of calcium chloride is 6%, and the amount of lecithin added is 3g. Lecithin helps to promote the uniform distribution between polysaccharide molecular chains, fills the coagulation structure of fat-reducing gel protective material, improves stability and protection, and lecithin also has the effect of decomposing emulsified oils and fats. Magnetic stirring is used to fully dissolve it for standby use. Then 6g sodium alginate and 5g pectin are added to 100mL water, stirred to fully hydrate it, 2g citric acid is added for cross-linking, the pH is adjusted to 6.0, the cross-linking temperature is 50°C, and the cross-linking time is 50min. The cross-linking of citric acid enhances the stability of sodium alginate and pectin, which is beneficial to enhancing the fat-reducing effect. The mechanical properties of the fat gel protective material further improve the coating of the probiotics. Cool to room temperature, then add the composite culture and the Chinese herbal prebiotic complex described in step S1, stir evenly, and use a syringe automatic propulsion pump to drip into the calcium chloride lecithin solution at a rate of 1 mL / min. Let it stand and solidify for 10 minutes, filter with a 200-mesh gauze filter, and freeze-dry the precipitate. The fat-reducing gel protective material wraps the composite culture and the Chinese herbal prebiotic complex, further enhancing the protective effect on the probiotics, and can be released at a fixed point in the colon, reducing the damage to the probiotics by the gastrointestinal tract, thereby also improving the weight-loss effect of the probiotics, and obtaining a probiotic composition with a weight-loss effect.

[0063] In this example, the prepared modified celery IDF multifunctional carrier and the probiotic composition with weight loss effect were subjected to scanning electron microscopy to observe their microscopic morphology. Figure 1 This is a SEM image of the modified celery IDF multifunctional carrier prepared in Example 1, magnified 1000 times. Figure 2 This is a SEM image of the probiotic composition with weight loss effect prepared in Example 1, magnified 400 times. Figure 1 The modified celery IDF multifunctional carrier prepared in this embodiment presents a porous hollow structure, such as Figure 2 The surface of the probiotic composition with weight loss effect prepared in this embodiment is coated with a condensation structure.

[0064] Example 2

[0065] This embodiment proposes a probiotic composition with a weight loss effect, comprising the following components in parts by weight: 20 parts of modified celery IDF multifunctional carrier, 10 parts of fat-reducing gel protective material, 8 parts of traditional Chinese medicine prebiotic complex, and 1 part of Lactobacillus plantarum.

[0066] The modified celery IDF multifunctional carrier is a structural material obtained by jointly modifying celery insoluble fiber with subcritical water and alkaline hydrogen peroxide, and is used as a fermentation carrier of Lactobacillus plantarum.

[0067] The fat-reducing gel protective material comprises the following components in parts by weight: 15 parts of sodium alginate, 12 parts of pectin, 3 parts of citric acid, 6 parts of lecithin and 6 parts of calcium chloride.

[0068] The traditional Chinese medicine prebiotic complex comprises the following components in parts by weight: 3 parts of ginseng, 3 parts of wolfberry, 1 part of mulberry polyphenols and 1 part of inulin.

[0069] The preparation method of the modified celery IDF multifunctional carrier specifically comprises the following steps:

[0070] (1) washing and cutting celery, beating the celery with a material-water ratio of 1:1, passing the slurry through a 100-mesh sieve, removing celery juice, and collecting celery residue. The celery residue mainly includes cellulose, hemicellulose, lignin, chitosan, etc. The structure of the water-insoluble fiber has a large number of dense vascular bundles, which are filamentous, rough on the surface, and large specific surface area, which is conducive to the attachment of probiotic bacteria. The celery water-insoluble dietary fiber also has the effects of increasing satiety, helping to lose weight, and reducing edema, thereby obtaining celery IDF;

[0071] (2) 8 g of the celery IDF described in step (1) was added to 80 mL of distilled water, stirred and mixed, and then placed in a multifunctional stirrer of a reactor, treated at 180° C. for 10 min. After the treatment, the reactor was immediately cooled to room temperature with tap water, and solid residues were collected using a vacuum pump, washed with distilled water until neutral, dried, and ground. Subcritical water modification does not require the addition of additional chemical reagents, so it has the advantages of a clean reaction process and high extraction efficiency. High temperature degrades cell wall polysaccharides, so that the celery IDF has a loose porous structure and exposes adsorption sites, thereby obtaining subcritical water-modified celery IDF;

[0072] (3) 10 g of the subcritical water-modified celery IDF described in step (2) was added to 200 mL of hydrogen peroxide solution, the mass fraction of the hydrogen peroxide solution being 1.0%. The addition of hydrogen peroxide can degrade the biomass connecting bonds in lignin, expose the internal structure and active groups, and enhance the adsorption capacity. A network structure appeared in the celery IDF after subcritical water modification, which reduced the interaction between cellulose, hemicellulose and lignin, and was more conducive to the entry of hydrogen peroxide into the interior to play a role, thereby facilitating a more thorough destruction of the connecting bonds between hemicellulose and lignin. 5 mo l / L sodium hydroxide solution was used to adjust the pH to 11.0, and then placed in a constant temperature water bath and stirred for 3 hours, the temperature was controlled at 70℃, and then the pH was adjusted to neutral with 1mol / L hydrochloric acid solution. After the end, a vacuum pump was used to collect the solid residue, washed twice with distilled water, dried, ground, and modified by subcritical water and hydrogen peroxide. A large amount of cellulose was exposed, so that the celery IDF had more adsorption sites and the internal structure was opened, which was conducive to the attachment of probiotics and played a protective role, further promoting the proliferation of probiotics, and obtaining a modified celery IDF multifunctional carrier.

[0073] The preparation method of the traditional Chinese medicine prebiotic complex specifically comprises the following steps:

[0074] a. Add water to ginseng and wolfberry and soak them in a decoction machine for 2 hours. Soaking is beneficial to the leaching of effective ingredients of traditional Chinese medicine and improves the extraction efficiency. Decoction is performed once, and the extraction is performed for 2 hours with slight boiling. The decoction is combined, and the filter residue is discarded. The collected filtrate is concentrated, and the vacuum pressure is -0.06MPa and the temperature is 40°C. The filtrate is dried and crushed. Ginseng and wolfberry contain active ingredients such as saponins and polysaccharides, which have significant prebiotic properties and can promote the growth of intestinal bacteria such as Lactobacillus and Bifidobacterium to obtain a traditional Chinese medicine extract;

[0075] b. Centrifuge the mulberry juice at 4000 rpm for 10 min at 4°C, slowly pour the supernatant into the chromatography column loaded with X-5 macroporous resin, and after the macroporous resin is saturated with adsorption, wash it with distilled water to remove sugar, acid and other water-soluble impurities, and then elute it with an acidic ethanol solution. In the acidic ethanol solution, the volume ratio of anhydrous ethanol, water and hydrochloric acid solution is 80:19:1. The acidic ethanol solution can fully elute the phenolic substances in the macroporous resin. Collect the eluate, and concentrate it under reduced pressure in a rotary evaporator until all ethanol is recovered. The concentration temperature is controlled at 38°C, and freeze-dried. Mulberry polyphenols have prebiotic properties, can regulate intestinal microecology, have the effect of promoting beneficial intestinal bacteria, and play a synergistic function through interaction with intestinal microorganisms. Mulberry polyphenols can also regulate the body's lipid metabolism, affect the proliferation, differentiation and apoptosis of fat cells, stimulate the body's heat production and accelerate energy consumption, and obtain mulberry polyphenols;

[0076] c. Disperse 1 g of inulin in 10 mL of pure water and stir magnetically for 1 h. Then add 1 g of the mulberry polyphenols described in step b and 3 g of the traditional Chinese medicine extract described in step a. The combination of mulberry polyphenols, inulin and traditional Chinese medicine extract can protect the polyphenols from degradation and improve stability. Mulberry polyphenols and inulin also give the traditional Chinese medicine extract multiple prebiotic effects. Continue magnetic stirring for 3 h, freeze-dry, and obtain a traditional Chinese medicine prebiotic complex.

[0077] This embodiment provides a method for preparing a probiotic composition having a weight loss effect, which specifically comprises the following steps:

[0078] S1. Inoculate 1g of plant lactobacillus into a blue-mouth bottle containing MRS culture medium and modified celery IDF multifunctional carrier and culture for 36 hours. The modified celery IDF multifunctional carrier can effectively enrich probiotics, has a good protective effect on the activity of probiotics, and reduces the adverse effects of the external environment on the activity of probiotics. Filter and remove the supernatant. Put the modified celery IDF multifunctional carrier containing plant lactobacillus into a fermentation tank, add MRS culture medium to reach 80% of the tank volume, and culture at 37°C for 7 days. Replace the MRS culture medium in the tank every 1 day. After the cultivation is completed, discard the supernatant and freeze-dry. At this time, the content of plant lactobacillus is 13LogCFU / g. Utilize the principle of biofilm enrichment of microorganisms to ferment and culture the modified celery IDF multifunctional carrier and probiotics together. Not only the probiotic effect of the culture is enhanced, but also the tolerance to gastrointestinal digestive juice is effectively improved, which is conducive to better maintaining the activity of probiotics, thereby achieving an ideal weight loss effect and obtaining a composite culture.

[0079] S2, lecithin and calcium chloride are added to 50mL water, wherein the mass fraction of calcium chloride is 4%, and the amount of lecithin added is 2g. Lecithin helps to promote the uniform distribution between polysaccharide molecular chains, fills the coagulation structure of fat-reducing gel protective material, improves stability and protection, and lecithin also has the function of decomposing emulsified oils and fats. Magnetic stirring is used to fully dissolve it for standby use. Then 5g sodium alginate and 4g pectin are added to 80mL water, stirred to fully hydrate it, 1g citric acid is added for cross-linking, the pH is adjusted to 5.0, the cross-linking temperature is 40°C, and the cross-linking time is 30min. The cross-linking of citric acid enhances the stability of sodium alginate and pectin, which is beneficial to enhance the fat-reducing effect. The mechanical properties of the fat gel protective material further improve the coating of the probiotics. Cool to room temperature, then add the composite culture and the Chinese herbal prebiotic complex described in step S1, stir evenly, and use a syringe automatic propulsion pump to drip into the calcium chloride lecithin solution at a rate of 1 mL / min. Let it stand and solidify for 5 minutes, filter with a 200-mesh gauze filter, and freeze-dry the precipitate. The fat-reducing gel protective material wraps the composite culture and the Chinese herbal prebiotic complex, further enhancing the protective effect on the probiotics, and can be released at a fixed point in the colon, reducing the damage to the probiotics by the gastrointestinal tract, thereby also improving the weight-loss effect of the probiotics, and obtaining a probiotic composition with a weight-loss effect.

[0080] Example 3

[0081] This embodiment proposes a probiotic composition with a weight loss effect, comprising the following components in parts by weight: 25 parts of modified celery IDF multifunctional carrier, 15 parts of fat-reducing gel protective material, 10 parts of traditional Chinese medicine prebiotic complex, and 1.5 parts of Lactobacillus plantarum.

[0082] The modified celery IDF multifunctional carrier is a structural material obtained by jointly modifying celery insoluble fiber with subcritical water and alkaline hydrogen peroxide, and is used as a fermentation carrier of Lactobacillus plantarum.

[0083] The fat-reducing gel protective material comprises the following components in parts by weight: 16.5 parts of sodium alginate, 13.5 parts of pectin, 4.5 parts of citric acid, 7.5 parts of lecithin and 7.5 parts of calcium chloride.

[0084] The traditional Chinese medicine prebiotic complex comprises the following components in parts by weight: 4 parts of ginseng, 4 parts of wolfberry, 1.5 parts of mulberry polyphenols and 1.5 parts of inulin.

[0085] The preparation method of the modified celery IDF multifunctional carrier specifically comprises the following steps:

[0086] (1) washing and cutting celery, beating the celery in a material-water ratio of 1:1, passing the slurry through a 150-mesh sieve, removing celery juice, and collecting celery residue. The celery residue mainly includes cellulose, hemicellulose, lignin, chitosan, etc. The structure of the water-insoluble fiber has a large number of dense vascular bundles, which are filamentous, rough on the surface, and large specific surface area, which is conducive to the attachment of probiotic bacteria. The celery water-insoluble dietary fiber also has the effects of increasing satiety, helping to lose weight, and reducing edema, thereby obtaining celery IDF;

[0087] (2) 8 g of the celery IDF described in step (1) was added to 90 mL of distilled water, stirred and mixed, and then placed in a multifunctional stirrer of a reactor, treated at 200° C. for 20 min. After the treatment, the reactor was immediately cooled to room temperature with tap water, and solid residues were collected using a vacuum pump, washed with distilled water until neutral, dried, and ground. Subcritical water modification does not require the addition of additional chemical reagents, so it has the advantages of a clean reaction process and high extraction efficiency. High temperature degrades cell wall polysaccharides, so that the celery IDF has a loose porous structure and exposes adsorption sites, thereby obtaining subcritical water-modified celery IDF;

[0088] (3) 10 g of the subcritical water-modified celery IDF described in step (2) was added to 200 mL of hydrogen peroxide solution, the mass fraction of the hydrogen peroxide solution being 1.5%. The addition of hydrogen peroxide can degrade the biomass connecting bonds in lignin, expose the internal structure and active groups, and enhance the adsorption capacity. A network structure appeared in the celery IDF after subcritical water modification, which reduced the interaction between cellulose, hemicellulose and lignin, and was more conducive to the entry of hydrogen peroxide into the interior to play a role, thereby facilitating a more thorough destruction of the connecting bonds between hemicellulose and lignin. 5 mol / L of sodium hydroxide solution was used to adjust the pH to 11.0, and then placed in a constant temperature water bath and stirred for 3.5 hours, the temperature was controlled at 75℃, and then the pH was adjusted to neutral with 1mol / L hydrochloric acid solution. After the end, a vacuum pump was used to collect the solid residue, washed twice with distilled water, dried, ground, and modified by subcritical water and hydrogen peroxide. A large amount of cellulose was exposed, so that the celery IDF had more adsorption sites and the internal structure was opened, which was conducive to the attachment of probiotics and played a protective role, further promoting the proliferation of probiotics, and obtaining a modified celery IDF multifunctional carrier.

[0089] The preparation method of the traditional Chinese medicine prebiotic complex specifically comprises the following steps:

[0090] a. Add water to ginseng and wolfberry and soak them in a decoction machine for 3 hours. Soaking is beneficial to the leaching of effective ingredients of traditional Chinese medicine and improves the extraction efficiency. Decoction is performed once, and the extraction is performed at a slight boiling point for 2.5 hours. The decoction is combined, and the filter residue is discarded. The collected filtrate is concentrated, and the vacuum pressure is -0.06MPa and the temperature is 45°C. The filtrate is dried and crushed. Ginseng and wolfberry contain active ingredients such as saponins and polysaccharides, which have significant prebiotic properties and can promote the growth of intestinal bacteria such as Lactobacillus and Bifidobacterium to obtain a traditional Chinese medicine extract;

[0091] b. Centrifuge the mulberry juice at 4000 rpm for 10 min at 4°C, slowly pour the supernatant into the chromatography column loaded with X-5 macroporous resin, and after the macroporous resin is saturated with adsorption, wash it with distilled water to remove sugar, acid and other water-soluble impurities, and then elute it with an acidic ethanol solution. In the acidic ethanol solution, the volume ratio of anhydrous ethanol, water and hydrochloric acid solution is 80:19:1. The acidic ethanol solution can fully elute the phenolic substances in the macroporous resin. Collect the eluate, and concentrate it under reduced pressure in a rotary evaporator until all ethanol is recovered. Control the concentration temperature at 40°C and freeze-dry. Mulberry polyphenols have prebiotic properties, can regulate intestinal microecology, have the effect of promoting beneficial intestinal bacteria, and play a synergistic function through interaction with intestinal microorganisms. Mulberry polyphenols can also regulate the body's lipid metabolism, affect the proliferation, differentiation and apoptosis of fat cells, stimulate the body's heat production and accelerate energy consumption, and obtain mulberry polyphenols;

[0092] c. Disperse 1.5 g of inulin in 10 mL of pure water and stir magnetically for 1.5 h. Then add 1.5 g of the mulberry polyphenols described in step b and 4 g of the traditional Chinese medicine extract described in step a. The combination of mulberry polyphenols, inulin and traditional Chinese medicine extract can protect the polyphenols from degradation and improve stability. Mulberry polyphenols and inulin also give the traditional Chinese medicine extract multiple prebiotic effects. Continue magnetic stirring for 3.5 h, freeze-dry, and obtain a traditional Chinese medicine prebiotic complex.

[0093] This embodiment provides a method for preparing a probiotic composition having a weight loss effect, which specifically comprises the following steps:

[0094] S1. Inoculate 1.5 g of plant lactobacillus into a blue-mouth bottle containing MRS culture medium and modified celery IDF multifunctional carrier and culture for 36 hours. The modified celery IDF multifunctional carrier can effectively enrich probiotics, has a good protective effect on the activity of probiotics, and reduces the adverse effects of the external environment on the activity of probiotics. Filter and remove the supernatant. Put the modified celery IDF multifunctional carrier containing plant lactobacillus into a fermentation tank, add MRS culture medium to reach 80% of the tank volume, and culture at 37°C for 7 days. Replace the MRS culture medium in the tank every 1 day. After the cultivation is completed, discard the supernatant and freeze-dry. At this time, the content of plant lactobacillus is 14LogCFU / g. Utilize the principle of biofilm enrichment of microorganisms to ferment and culture the modified celery IDF multifunctional carrier and probiotics together. Not only the probiotic effect of the culture is enhanced, but also the tolerance to gastrointestinal digestive juice is effectively improved, which is conducive to better maintaining the activity of probiotics, thereby achieving an ideal weight loss effect and obtaining a composite culture.

[0095] S2. Add lecithin and calcium chloride to 50 mL of water, wherein the mass fraction of calcium chloride is 5% and the amount of lecithin added is 2.5 g. Lecithin helps to promote the uniform distribution between polysaccharide molecular chains, fill the coagulation structure of the fat-reducing gel protective material, and improve stability and protection. Lecithin also has the function of decomposing and emulsifying oils. Stir magnetically to fully dissolve it for standby use. Then add 5.5 g of sodium alginate and 4.5 g of pectin to 90 mL of water, stir to fully hydrate it, add 1.5 g of citric acid for cross-linking, adjust the pH to 5.5, the cross-linking temperature to 45 ° C, and the cross-linking time to 40 min. The cross-linking of citric acid enhances the stability of sodium alginate and pectin, which is beneficial to The mechanical properties of the fat-reducing gel protective material are enhanced, and the coating of the probiotics is further improved. The material is cooled to room temperature, and then the composite culture and the Chinese herbal prebiotic complex described in step S1 are added, stirred evenly, and dripped into the calcium chloride lecithin solution at a rate of 1 mL / min using a syringe automatic propulsion pump. The material is allowed to stand and solidify for 7.5 minutes, filtered with a 200-mesh gauze filter, and the precipitate is freeze-dried. The fat-reducing gel protective material wraps the composite culture and the Chinese herbal prebiotic complex, further enhancing the protective effect on the probiotics, and can be released at a fixed point in the colon, reducing the damage to the probiotics by the gastrointestinal tract, thereby also improving the weight-loss effect of the probiotics, and obtaining a probiotic composition with a weight-loss effect.

[0096] Example 4

[0097] This embodiment proposes a probiotic composition with a weight loss effect, comprising the following components in parts by weight: 30 parts of modified celery IDF multifunctional carrier, 10 parts of fat-reducing gel protective material, 12 parts of traditional Chinese medicine prebiotic complex, and 2 parts of Lactobacillus plantarum.

[0098] The modified celery IDF multifunctional carrier is a structural material obtained by jointly modifying celery insoluble fiber with subcritical water and alkaline hydrogen peroxide, and is used as a fermentation carrier of Lactobacillus plantarum.

[0099] The fat-reducing gel protective material comprises the following components in parts by weight: 18 parts of sodium alginate, 15 parts of pectin, 3 parts of citric acid, 6 parts of lecithin and 9 parts of calcium chloride.

[0100] The traditional Chinese medicine prebiotic complex comprises the following components in parts by weight: 5 parts of ginseng, 5 parts of wolfberry, 1 part of mulberry polyphenols and 1 part of inulin.

[0101] The preparation method of the modified celery IDF multifunctional carrier specifically comprises the following steps:

[0102] (1) washing and cutting celery, beating the celery with a material-water ratio of 1:1, passing the slurry through a 200-mesh sieve, removing celery juice, and collecting celery residue. The celery residue mainly includes cellulose, hemicellulose, lignin, chitosan, etc. The structure of the water-insoluble fiber has a large number of dense vascular bundles, which are filamentous, rough on the surface, and large specific surface area, which is conducive to the attachment of probiotic bacteria. The celery water-insoluble dietary fiber also has the effects of increasing satiety, helping to lose weight, and reducing edema, thereby obtaining celery IDF;

[0103] (2) 8 g of the celery IDF described in step (1) was added to 100 mL of distilled water, stirred and mixed, and then placed in a multifunctional stirrer of a reactor, and treated at 220° C. for 10 min. After the treatment, the reactor was immediately cooled to room temperature with tap water, and solid residues were collected using a vacuum pump, washed with distilled water until neutral, dried, and ground. Subcritical water modification does not require the addition of additional chemical reagents, so it has the advantages of a clean reaction process and high extraction efficiency. High temperature degrades cell wall polysaccharides, so that the celery IDF has a loose porous structure and exposes adsorption sites, thereby obtaining subcritical water-modified celery IDF;

[0104] (3) 10 g of the subcritical water-modified celery IDF described in step (2) was added to 200 mL of hydrogen peroxide solution, the mass fraction of the hydrogen peroxide solution being 2.0%. The addition of hydrogen peroxide can degrade the biomass connecting bonds in lignin, expose the internal structure and active groups, and enhance the adsorption capacity. A network structure appeared in the celery IDF after subcritical water modification, which reduced the interaction between cellulose, hemicellulose and lignin, and was more conducive to the entry of hydrogen peroxide into the interior to play a role, thereby facilitating a more thorough destruction of the connecting bonds between hemicellulose and lignin. 5 mo l / L sodium hydroxide solution was used to adjust the pH to 11.0, and then placed in a constant temperature water bath and stirred for 3 hours, the temperature was controlled at 80℃, and then the pH was adjusted to neutral with 1mol / L hydrochloric acid solution. After the end, a vacuum pump was used to collect the solid residue, washed with distilled water 3 times, dried, ground, and modified by subcritical water and hydrogen peroxide. A large amount of cellulose was exposed, so that the celery IDF had more adsorption sites and the internal structure was opened, which was conducive to the attachment of probiotics and played a protective role, further promoting the proliferation of probiotics, and obtaining a modified celery IDF multifunctional carrier.

[0105] The preparation method of the traditional Chinese medicine prebiotic complex specifically comprises the following steps:

[0106] a. Add water to ginseng and wolfberry and soak them in a decoction machine for 2 hours. Soaking is beneficial to the leaching of effective ingredients of traditional Chinese medicine and improves the extraction efficiency. Decoction is performed twice, and the extraction is performed at a slight boiling temperature for 2 hours. The decoction is combined, and the filter residue is discarded. The collected filtrate is concentrated, and the vacuum pressure is -0.06MPa and the temperature is 50°C. The filtrate is dried and crushed. Ginseng and wolfberry contain active ingredients such as saponins and polysaccharides, which have significant prebiotic properties and can promote the growth of intestinal bacteria such as Lactobacillus and Bifidobacterium to obtain a traditional Chinese medicine extract;

[0107] b. Centrifuge the mulberry juice at 4000 rpm for 10 min at 4°C, slowly pour the supernatant into the chromatography column loaded with X-5 macroporous resin, and after the macroporous resin is saturated with adsorption, wash it with distilled water to remove sugar, acid and other water-soluble impurities, and then elute it with an acidic ethanol solution. In the acidic ethanol solution, the volume ratio of anhydrous ethanol, water and hydrochloric acid solution is 80:19:1. The acidic ethanol solution can fully elute the phenolic substances in the macroporous resin. Collect the eluate, and concentrate it under reduced pressure in a rotary evaporator until all ethanol is recovered. Control the concentration temperature at 42°C and freeze-dry. Mulberry polyphenols have prebiotic properties, can regulate intestinal microecology, have the effect of promoting beneficial intestinal bacteria, and play a synergistic function through interaction with intestinal microorganisms. Mulberry polyphenols can also regulate the body's lipid metabolism, affect the proliferation, differentiation and apoptosis of fat cells, stimulate the body's heat production and accelerate energy consumption, and obtain mulberry polyphenols;

[0108] c. Disperse 1 g of inulin in 10 mL of pure water and stir magnetically for 1 h. Then add 1 g of the mulberry polyphenols described in step b and 5 g of the traditional Chinese medicine extract described in step a. The combination of mulberry polyphenols, inulin and traditional Chinese medicine extract can protect the polyphenols from degradation and improve stability. Mulberry polyphenols and inulin also give the traditional Chinese medicine extract multiple prebiotic effects. Continue magnetic stirring for 3 h and freeze-dry to obtain a traditional Chinese medicine prebiotic complex.

[0109] This embodiment provides a method for preparing a probiotic composition having a weight loss effect, which specifically comprises the following steps:

[0110] S1. Inoculate 2g of plant lactobacillus into a blue-mouth bottle containing MRS culture medium and modified celery IDF multifunctional carrier and culture for 36h. The modified celery IDF multifunctional carrier can effectively enrich probiotics, has a good protective effect on the activity of probiotics, and reduces the adverse effects of the external environment on the activity of probiotics. Filter and remove the supernatant. Put the modified celery IDF multifunctional carrier containing plant lactobacillus into a fermentation tank, add MRS culture medium to reach 80% of the tank volume, and culture at 37°C for 7d. Replace the MRS culture medium in the tank every 1d. After the cultivation is completed, discard the supernatant and freeze-dry. At this time, the content of plant lactobacillus is 13LogCFU / g. Utilize the principle of biofilm enrichment of microorganisms to ferment and culture the modified celery IDF multifunctional carrier and probiotics together. Not only the probiotic effect of the culture is enhanced, but also the tolerance to gastrointestinal digestive juice is effectively improved, which is conducive to better maintaining the activity of probiotics, thereby achieving an ideal weight loss effect and obtaining a composite culture.

[0111] S2, lecithin and calcium chloride are added to 50mL water, wherein the mass fraction of calcium chloride is 6%, and the amount of lecithin added is 2g. Lecithin helps to promote the uniform distribution between polysaccharide molecular chains, fills the coagulation structure of the fat-reducing gel protective material, improves stability and protection, and lecithin also has the function of decomposing emulsified oils. Magnetic stirring is used to fully dissolve it for standby use. Then 6g sodium alginate and 5g pectin are added to 100mL water, stirred to fully hydrate it, 1g citric acid is added for cross-linking, the pH is adjusted to 6.0, the cross-linking temperature is 50°C, and the cross-linking time is 30min. The cross-linking of citric acid enhances the stability of sodium alginate and pectin, which is beneficial to enhance The mechanical properties of the fat-reducing gel protective material further improve the coating of probiotics. Cool to room temperature, then add the composite culture and Chinese herbal prebiotic complex described in step S1, stir evenly, and use a syringe automatic propulsion pump to drip into the calcium chloride lecithin solution at a rate of 1 mL / min. Let it stand and solidify for 5 minutes, filter with a 200-mesh gauze filter, and freeze-dry the precipitate. The fat-reducing gel protective material wraps the composite culture and the Chinese herbal prebiotic complex, further enhancing the protective effect on probiotics, and can be released at a fixed point in the colon, reducing the damage to the probiotics by the gastrointestinal tract, thereby also improving the weight-loss effect of the probiotics, and obtaining a probiotic composition with a weight-loss effect.

[0112] Comparative Example 1

[0113] This comparative example provides a probiotic composition with a weight loss effect, which is different from Example 1 in that the modified celery IDF multifunctional carrier does not contain subcritical water and alkaline hydrogen peroxide modification treatment; the preparation method of the modified celery IDF multifunctional carrier does not include steps (2) and (3); the preparation method of the Chinese medicine prebiotic complex is the same as that of Example 1; and the preparation method of the probiotic composition with a weight loss effect is the same as that of Example 1.

[0114] Comparative Example 2

[0115] This comparative example provides a probiotic composition with a weight loss effect, which is different from Example 1 in that the fat-reducing gel protective material does not contain pectin, lecithin, and citric acid; the preparation method of the modified celery IDF multifunctional carrier is the same as that of Example 1; the preparation method of the Chinese medicine prebiotic complex is the same as that of Example 1; and the preparation method of the probiotic composition with a weight loss effect is the same as that of Example 1.

[0116] Comparative Example 3

[0117] This comparative example provides a probiotic composition with a weight loss effect, which is different from Example 1 in that the probiotic composition with a weight loss effect does not contain pectin, lecithin, citric acid, and the celery IDF multifunctional carrier is not modified by subcritical water and alkaline hydrogen peroxide; the preparation method of the modified celery IDF multifunctional carrier does not include steps (2) and (3); the preparation method of the Chinese medicine prebiotic complex is the same as that of Example 1; and the preparation method of the probiotic composition with a weight loss effect is the same as that of Example 1.

[0118] Experimental Example 1

[0119] Storage activity test

[0120] Test sample: the probiotic composition with weight loss effect prepared in Examples 1-4 and Comparative Examples 1-3.

[0121] Test method: Collect 5 g of the test sample in a sterile glass bottle and store it at 25°C for 8 weeks. Take samples every 2 weeks and use the plate count method to determine the number of viable bacteria (LogCFU / g) in the sample at 0 weeks, 2 weeks, 4 weeks, 6 weeks, and 8 weeks.

[0122] Figure 3The results of the viable bacterial counts during the storage period of Examples 1-4 and Comparative Examples 1-3 are shown in FIG. As shown in the figure, the viable bacterial counts at 0w, 2w, 4w, 6w, and 8w of Examples 1-4 are 13.0-14.0LogCFU / g, 12.7-13.8LogCFU / g, 12.2-13.7LogCFU / g, 11.8-13.5LogCFU / g, and 11.5-13.3LogCFU / g, respectively, indicating that the storage period is beneficial. The probiotic activity was maintained well; the viable bacterial counts of 0w, 2w, 4w, 6w, and 8w of Comparative Examples 1-3 were 13.0-14.0LogCFU / g, 12.4-12.7LogCFU / g, 11.2-11.9LogCFU / g, 9.5-11.0LogCFU / g, and 8.5-10.2LogCFU / g, respectively, indicating that the probiotic activity was poor during storage; the modified celery IDF of Comparative Example 1 was more The functional carrier does not contain subcritical water and alkaline hydrogen peroxide modification treatment, which is not conducive to the enrichment of probiotics on the carrier surface or in the cavity, weakens the dual protective effect of the carrier and the fat-reducing gel protective material, and causes the probiotic activity to remain poor during the storage period; the fat-reducing gel protective material of comparative example 2 does not contain pectin, lecithin, and citric acid, which is not conducive to the uniform cross-linking of polysaccharides, weakens the mechanical properties and stability of the fat-reducing gel protective material, and is not conducive to effective isolation of the external environment, resulting in poor probiotic activity during the storage period; the probiotic composition with weight loss effect of comparative example 3 does not contain pectin, lecithin, citric acid, and the celery IDF multifunctional carrier is not modified with subcritical water and alkaline hydrogen peroxide, which is not conducive to the density and stability of the external protective film, and the enrichment protection of the modified celery IDF multifunctional carrier on probiotics cannot be exerted, and the dual protection effect is adversely affected, resulting in poor probiotic activity during the storage period.

[0123] Experimental Example 2

[0124] In vitro simulated gastrointestinal digestion experiment

[0125] Test sample: the probiotic composition with weight loss effect prepared in Examples 1-4 and Comparative Examples 1-3.

[0126] Test method: Preparation of simulated gastric digestive fluid: add 3.2 mg / mL of pepsin to a mixed solution containing 2 mg / mL NaCl and 7 mL / L HCl, and adjust the pH to 2.0 for standby use;

[0127] Preparation of simulated intestinal digestive fluid: weigh 36.7g calcium chloride and 218.7g sodium chloride, dissolve them in distilled water and make up to 1L for later use;

[0128] 5 g of the test sample was added to 9.9 mL of simulated gastric digestive fluid and placed in a water bath at 37°C and 100 r / min for 2 h. After gastric digestion, the pH value of the mixed solution was adjusted to 7.0 with 0.25 mol / L NaOH, and 1.5 mL of simulated intestinal digestive fluid, 189 mg of ox bile salt (dissolved in 3.5 mL of 5 mmol / L PBS buffer, pH = 7.0), 60 mg of lipase and 60 mg of trypsin (dissolved in 2.5 mL of 5 mmol / L PBS buffer, pH = 7.0) were added. The mixed solution was continued to be placed in a water bath at 37°C and 100 r / min for 2 h. The digestive fluids after gastric and intestinal digestion were taken respectively, and 0.5% sodium citrate solution was added to decapsulate the capsules, then diluted with physiological saline and spread on MRS culture medium. The colony count (Log CFU / g) was performed after aerobic culture at 37°C for 48 h.

[0129] Figure 4 The figure is a result diagram of the number of live bacteria after simulated gastrointestinal digestion of Examples 1-4 and Comparative Examples 1-3; as shown in the figure, the number of live bacteria after simulated gastrointestinal digestion of Examples 1-4 is 12.5-13.5LogCFU / g and 12.0-13.1LogCFU / g, respectively, indicating that the tolerance to gastrointestinal digestive juice is good; the number of live bacteria after simulated gastrointestinal digestion of Comparative Examples 1-3 is 9.7-11.3LogCFU / g and 8.2-10.1LogCFU / g, respectively, indicating that the tolerance to gastrointestinal digestive juice is poor; the modified celery IDF multifunctional carrier of Comparative Example 1 does not contain subcritical water and alkaline hydrogen peroxide modification, which is not conducive to the attachment of probiotics and weakens the The resistance of the gastrointestinal tract results in poor tolerance to gastrointestinal digestive juice; the fat-reducing gel protective material of Comparative Example 2 does not contain pectin, lecithin, and citric acid, which is not conducive to the density of the gel spherical structure, nor does it have colon targeting, which is not conducive to the protection of probiotics in the gastrointestinal tract, resulting in poor tolerance to gastrointestinal digestive juice; the probiotic composition with weight loss effect of Comparative Example 3 does not contain pectin, lecithin, citric acid, and the celery IDF multifunctional carrier is not modified by subcritical water and alkaline hydrogen peroxide. It has neither colon targeting effect nor can it reduce the decomposition of the digestive juice by the probiotics by attaching and enriching on the carrier surface or in the cavity, resulting in poor tolerance to the gastrointestinal digestive juice.

[0130] Experimental Example 3

[0131] Weight loss effect experiment

[0132] Test sample: the probiotic composition with weight loss effect prepared in Examples 1-4 and Comparative Examples 1-3.

[0133] Test method: 4-week-old KM mice were selected, half male and half female, a total of 35 mice, 5 mice in each group, and fed with a high-fat diet (the formula of the high-fat diet was 1 kg casein, 15 g L-cysteine, 0.75 kg maltodextrin, 0.35 kg sucrose, 0.25 kg cellulose, 0.15 kg soybean oil, and 1.25 kg lard). After 6 weeks of feeding, mice weighing less than 60 g were eliminated, and then the mice were divided into groups and given the corresponding test samples (500 mg / kg) by gavage for 6 consecutive weeks. During this period, they were still fed with a high-fat diet. The body weight was weighed in the 6th week and the average weight (kg) of each group of mice was calculated.

[0134] Figure 5 The weight results of mice in Examples 1-4 and Comparative Examples 1-3 are shown in the figure; as shown in the figure, the weight of mice in Examples 1-4 is 46-50kg, indicating that the weight loss effect is better; the weight of mice in Comparative Examples 1-3 is 53-58kg, indicating that the weight loss effect is not good; the modified celery IDF multifunctional carrier in Comparative Example 1 does not contain subcritical water and alkaline hydrogen peroxide modification treatment, and cannot effectively enrich probiotics, thereby reducing the activity of probiotics, thereby limiting its improvement on intestinal microbial flora, resulting in poor weight loss effect; the fat-reducing gel protective material in Comparative Example 2 does not contain Pectin, lecithin and citric acid weaken the protection of the external gel structure on the activity of probiotics, and cannot play the role of pectin, lecithin and citric acid in assisting fat reduction, resulting in poor weight loss effect; the probiotic composition with weight loss effect in comparative example 3 does not contain pectin, lecithin, citric acid, and the celery IDF multifunctional carrier is not modified by subcritical water and alkaline hydrogen peroxide, which can neither maintain high-activity probiotics nor be conducive to absorption and utilization in the colon after entering the human body, thereby weakening the conditioning effect of probiotics on the intestine, resulting in poor weight loss effect.

[0135] The above experimental results show that the storage activity, gastrointestinal tolerance and weight loss effect of Examples 1-4 of the present invention are significantly better than those of Comparative Examples 1-3 samples. Among them, Example 1 using the modified celery IDF multifunctional carrier and the fat-reducing gel protective material has better storage activity and gastrointestinal tolerance and better weight loss effect. The modified celery IDF multifunctional carrier has more adsorption sites, which is conducive to the attachment and enrichment of probiotics on the carrier surface or in the cavity, reducing the adverse effects of the external environment on the activity of probiotics, and has a certain digestibility resistance, which can reduce the digestion and decomposition of probiotics after entering the gastrointestinal tract, thereby achieving protection of the activity and quantity of probiotics. The dual protective effect of the modified celery IDF multifunctional carrier and the fat-reducing gel protective material significantly improves the activity of probiotics, reduces digestion and decomposition in the gastrointestinal tract, is conducive to the absorption and utilization of probiotics and prebiotics in the colon, and plays a weight loss effect by improving the growth and reproduction of intestinal flora.

[0136] While the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that many changes, modifications, substitutions and alterations may be made to the embodiments without departing from the principles and spirit of the invention.

[0137] The present invention and its implementation methods are described above, which is not restrictive. The drawings are only one of the implementation methods of the present invention, and the actual application is not limited thereto. In short, if ordinary technicians in the field are inspired by it and design methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention, they should all fall within the protection scope of the present invention.

Claims

1. A probiotic composition with weight loss effect, characterized in that: The probiotic composition with weight loss effect is made of the following components in parts by weight: 20-30 parts of modified celery IDF multifunctional carrier, 10-20 parts of fat-reducing gel protective material, 8-12 parts of traditional Chinese medicine prebiotic complex, and 1-2 parts of plant lactobacillus; the modified celery IDF multifunctional carrier is a structural material obtained by jointly modifying celery insoluble fiber with subcritical water and alkaline hydrogen peroxide, and is used as a fermentation carrier of plant lactobacillus; the fat-reducing gel protective material is made of the following components in parts by weight: 15-18 parts of sodium alginate, 12-15 parts of pectin, 3-6 parts of citric acid, 6-9 parts of lecithin, and 6-9 parts of calcium chloride; the traditional Chinese medicine prebiotic complex is made of the following components in parts by weight: 3-5 parts of ginseng, 3-5 parts of wolfberry, 1-2 parts of mulberry polyphenols, and 1-2 parts of inulin; The preparation method of the modified celery IDF multifunctional carrier specifically comprises the following steps: (1) Wash and cut the celery, and pulp it according to the material-water ratio of 1:

1. The pulp is passed through a 100-200 mesh sieve to remove the celery juice, and the celery residue is collected to obtain celery IDF; (2) adding 8 g of the celery IDF described in step (1) into 80-100 mL of distilled water, stirring and mixing, and then placing in a multifunctional stirrer of a reactor, treating at 180-220° C. for 10-30 min, and immediately cooling the reactor to room temperature with tap water after the treatment, collecting the solid residue with a vacuum pump, washing with distilled water until neutral, drying, and grinding to obtain subcritical water modified celery IDF; (3) Add 10 g of the subcritical water-modified celery IDF described in step (2) to 200 mL of hydrogen peroxide solution, adjust the pH to 11.0 with 5 mol / L sodium hydroxide solution, and then place it in a constant temperature water bath and stir for 3-4 h, control the temperature to 70-80 ° C, and then adjust the pH to neutral with 1 mol / L hydrochloric acid solution. After the end, use a vacuum pump to collect the solid residue, wash it with distilled water 2-3 times, dry it, and grind it to obtain a modified celery IDF multifunctional carrier; In step (3), the mass fraction of the hydrogen peroxide solution is 1.0-2.0%.

2. The probiotic composition with weight loss effect according to claim 1, characterized in that: The preparation method of the traditional Chinese medicine prebiotics complex specifically comprises the following steps: a. Add water to ginseng and wolfberry and soak them in a decoction machine for 2-4 hours, decoct them 1-2 times, extract them with slight boiling for 2-3 hours, combine the decoctions, discard the filter residue, concentrate the collected filtrate, dry and crush them at a vacuum pressure of -0.06MPa and a temperature of 40-50°C to obtain a Chinese medicine extract; b. Centrifuge the mulberry juice at 4000 rpm for 10 min at 4° C., slowly pour the supernatant into a chromatography column loaded with X-5 macroporous resin, wash with distilled water after the macroporous resin is saturated with adsorption, and then elute with an acidic ethanol solution, collect the eluate, and concentrate it under reduced pressure in a rotary evaporator until all the ethanol is recovered, control the concentration temperature at 38-42° C., and freeze-dry to obtain mulberry polyphenols; c. Disperse 1-2 g of inulin in 10 mL of pure water, stir magnetically for 1-2 h, then add 1-2 g of mulberry polyphenols described in step b and 3-5 g of the Chinese medicine extract described in step a, continue magnetic stirring for 3-4 h, and freeze-dry to obtain a Chinese medicine prebiotic complex; In step b, in the acidic ethanol solution, the volume ratio of anhydrous ethanol, water and hydrochloric acid solution is 80:19:

1.

3. A method for preparing a probiotic composition having a weight loss effect according to claim 1 or claim 2, characterized in that: The specific steps include: S1, inoculating 1-2g of plant lactobacillus into a blue-mouth bottle filled with MRS medium and modified celery IDF multifunctional carrier and culturing for 36h, filtering, removing the supernatant, placing the modified celery IDF multifunctional carrier containing plant lactobacillus into a fermentation tank, adding MRS medium to reach 80% of the tank volume, culturing at 37°C for 7d, replacing the MRS medium in the tank every 1d, after the cultivation is completed, discarding the supernatant, freeze-drying, at this time the content of plant lactobacillus is 13-14LogCFU / g, and obtaining a composite culture; S2, add lecithin and calcium chloride to 50mL water, wherein the mass fraction of calcium chloride is 4-6%, stir magnetically to fully dissolve it, and set aside, then add 5-6g sodium alginate and 4-5g pectin to 80-100mL water, stir to completely hydrate it, add 1-2g citric acid for cross-linking, adjust the pH to 5.0-6.0, the cross-linking temperature is 40-50°C, the cross-linking time is 30-50min, cool to room temperature, then add the composite culture and the Chinese medicine prebiotic complex described in step S1, stir evenly, use a syringe automatic propulsion pump to drop into the calcium chloride lecithin solution at a speed of 1mL / min, let it stand and solidify for 5-10min, filter with a 200-mesh gauze filter, and freeze-dry the precipitate to obtain a probiotic composition with a weight-loss effect.

4. The method for preparing the probiotic composition with weight loss effect according to claim 3, characterized in that: In step S2, the amount of lecithin added is 2-3 g.

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