Preparation method of seaweed oligosaccharide

Abstract

The invention relates to a preparation method of seaweed oligosaccharide. According to the preparation method disclosed by the invention, the seaweed is firstly soaked in water and sodium chloride, so that the osmotic pressure of the seaweed is improved, subsequent leaching of contents is facilitated, and enzymolysis is performed after crushing and homogenizing treatment by a homogenizer, so that the enzymolysis efficiency is effectively improved, and the enzymolysis extraction efficiency is further improved; the whole preparation process is simplified, the activity of main components cannot be lost in the enzymolysis process, the prepared seaweed oligosaccharide is high in purity and good in water solubility, has good adsorbability and water holding capacity, contains a small amount of cytex chitosan oligosaccharide, amino acid, trace elements and small molecule peptide which are beneficial components, can be applied to the fields of agricultural fertilizer, feed and the like, and has a wide application prospect. The application is wide.

Description

Technical Field

[0001] The invention relates to the technical field of seaweed oligosaccharide preparation, in particular to a method for preparing seaweed oligosaccharide. Background Art

[0003] Seaweed oligosaccharides are linear or branched compounds with a degree of polymerization of 2 to 10 obtained by degrading seaweed polysaccharides. They are widely found in seaweeds such as brown algae, red algae, green algae and blue algae. Seaweed oligosaccharides have the functions of improving the utilization rate of chemical fertilizers, regulating plant growth, improving plant stress resistance, and reducing the incidence of plant diseases and insect pests. As a new type of green and environmentally friendly biofertilizer, they play an important role in agricultural production. Seaweed oligosaccharides can also increase the number of soil microorganisms and promote changes in the structure of soil microbial communities. Using them in microbial fertilizers can help improve the colonization ability of functional bacteria in the soil. Summary of the invention

[0004] In view of the problems existing in the prior art, the present invention discloses a method for preparing seaweed oligosaccharides, comprising the following steps: Step 1: Soak the seaweed in water for 30-50 hours, then add 12% sodium chloride to the total amount of the seaweed, stir evenly, soak for 1-3 hours, and then wash the seaweed at least 3 times; Step 2: crush the seaweed obtained in step 1, add purified water at a volume ratio of 1:30, and prepare the seaweed slurry by homogenizing; Step 3: Put the seaweed slurry into an enzymatic hydrolysis tank, add 25-30% of the volume of the seaweed slurry alginate lyase, perform enzymatic hydrolysis, and obtain an enzymatic hydrolysis product; Step 4: The enzymatic hydrolysis product is separated by ultrafiltration, and the separated liquid is vacuum compressed and dried to obtain seaweed oligosaccharides.

[0005] The obtained seaweed oligosaccharide has a purity of 90%, and the remaining components are seaweed oligosaccharide, amino acids, trace elements and small molecule peptides.

[0006] As a preferred embodiment of the present invention, the homogenizing pressure of the homogenizer in step 2 is 220-280 MPa, the temperature is controlled at 30-38° C., and the homogenizing time is 20-30 minutes.

[0007] As a preferred embodiment of the present invention, the temperature of the enzymatic hydrolysis treatment in step three is 23-25° C., and the enzymatic hydrolysis time is 3-4 hours.

[0008] As a preferred embodiment of the present invention, the pore size of the filter membrane used in the ultrafiltration separation in step 4 is 0.9-1.2 nm.

[0009] As a preferred embodiment of the present invention, the vacuum compression pressure in step 4 is 75-80Kpa and the temperature is 40-45°C.

[0010] As a preferred embodiment of the present invention, the drying treatment in step 4 adopts microwave drying, and the drying temperature is 75-80°C.

[0011] The beneficial effects of the present invention are as follows: the present invention uses seaweed to be soaked in water and sodium chloride first, so as to increase the osmotic pressure of the seaweed, facilitate the subsequent leaching of the contents, and then perform enzymolysis after crushing and homogenization by a homogenizer, so as to effectively improve the enzymolysis efficiency, thereby further improving the enzymolysis extraction efficiency; the entire preparation process is simplified, the activity of the main components will not be lost during the enzymolysis process, and the prepared seaweed oligosaccharides have high purity, good water solubility, good adsorption and water holding capacity, and a small amount of seaweed oligosaccharides, amino acids, trace elements, and small molecule peptides contained therein are also beneficial ingredients, which can be applied to the fields of agricultural fertilizers, feeds, etc., and are widely used. DETAILED DESCRIPTION

[0012] Example 1

[0013] The present invention discloses a method for preparing seaweed oligosaccharides, comprising the following steps: Step 1: Soak seaweed in water for 30 hours, then add 12% sodium chloride to the total amount of seaweed, stir evenly, soak for 1 hour and then wash the seaweed at least 3 times; Step 2: crush the seaweed obtained in step 1, add purified water at a volume ratio of 1:30, and use a homogenizer to process the seaweed slurry; wherein the homogenizing pressure of the homogenizer is 220 MPa, the temperature is controlled at 30° C., and the homogenizing time is 20 minutes; Step 3: Put the seaweed slurry into an enzymolysis tank, add 25% of the volume of the seaweed slurry alginate lyase, and perform enzymolysis to obtain an enzymolysis product; the enzymolysis temperature is 23° C., and the enzymolysis time is 3 hours; Step 4: ultrafiltration separation of the enzymatic hydrolysis product, the pore size of the filter membrane used for ultrafiltration separation is 0.9 nm, the separation liquid is vacuum compressed, the pressure of the vacuum compression is 75 Kpa, the temperature is 40 ° C, and seaweed oligosaccharides are obtained by drying, the drying treatment adopts microwave drying, and the drying temperature is 75 ° C; the obtained seaweed oligosaccharides have a purity of 90%, and the remaining components are seaweed oligosaccharides, amino acids, trace elements, and small molecule peptides.

[0014] Example 2

[0015] The present invention discloses a method for preparing seaweed oligosaccharides, comprising the following steps: Step 1: Soak the seaweed in water for 50 hours, then add 12% sodium chloride to the total amount of the seaweed, stir evenly, soak for 3 hours, and then wash the seaweed at least 3 times; Step 2: crush the seaweed obtained in step 1, add purified water at a volume ratio of 1:30, and use a homogenizer to process the seaweed slurry; wherein the homogenizing pressure of the homogenizer is 280 MPa, the temperature is controlled at 38° C., and the homogenization time is 30 minutes; Step 3: Put the seaweed slurry into an enzymolysis tank, add 30% of the volume of the seaweed slurry alginate lyase, and perform enzymolysis to obtain an enzymolysis product; the enzymolysis temperature is 25° C., and the enzymolysis time is 4 hours; Step 4: ultrafiltration separation of the enzymatic hydrolysis product, the pore size of the filter membrane used for ultrafiltration separation is 1.2 nm, the separation liquid is vacuum compressed, the pressure of the vacuum compression is 80 Kpa, the temperature is 45 ° C, and seaweed oligosaccharides are obtained by drying, the drying treatment adopts microwave drying, and the drying temperature is 80 ° C; the obtained seaweed oligosaccharides have a purity of 90%, and the remaining components are seaweed oligosaccharides, amino acids, trace elements, and small molecule peptides.

[0016] Example 3

[0017] The present invention discloses a method for preparing seaweed oligosaccharides, comprising the following steps: Step 1: Soak the seaweed in water for 40 hours, then add 12% sodium chloride to the total amount of the seaweed, stir evenly, soak for 2 hours, and then wash the seaweed at least 3 times; Step 2: crush the seaweed obtained in step 1, add purified water at a volume ratio of 1:30, and use a homogenizer to process the seaweed slurry; wherein the homogenizing pressure of the homogenizer is 250 MPa, the temperature is controlled at 35° C., and the homogenization time is 25 minutes; Step 3: Put the seaweed slurry into an enzymolysis tank, add 27% of the volume of the seaweed slurry alginate lyase, and perform enzymolysis to obtain an enzymolysis product; the enzymolysis temperature is 24° C., and the enzymolysis time is 3.5 hours; Step 4: ultrafiltration separation of the enzymatic hydrolysis product, the pore size of the filter membrane used for ultrafiltration separation is 1 nm, the separation liquid is vacuum compressed, the pressure of the vacuum compression is 78 Kpa, the temperature is 43 ° C, and seaweed oligosaccharides are obtained by drying, the drying treatment is microwave drying, and the drying temperature is 78 ° C; the obtained seaweed oligosaccharides have a purity of 90%, and the remaining components are seaweed oligosaccharides, amino acids, trace elements, and small molecule peptides.

[0018] Components not described in detail herein are prior art.

[0019] Although the specific embodiments of the present invention are described in detail above, the present invention is not limited to the above embodiments. Various changes can be made within the knowledge of ordinary technicians in this field without departing from the purpose of the present invention, and modifications or deformations without creative labor are still within the protection scope of the present invention.

Claims

1. A method for preparing seaweed oligosaccharides, characterized in that: The steps include: Step 1: Soak the seaweed in water for 30-50 hours, then add 12% sodium chloride to the total amount of the seaweed, stir evenly, soak for 1-3 hours, and then wash the seaweed at least 3 times; Step 2: crush the seaweed obtained in step 1, add purified water at a volume ratio of 1:30, and prepare the seaweed slurry by homogenizing; Step 3: Put the seaweed slurry into an enzymatic hydrolysis tank, add 25-30% of the volume of the seaweed slurry alginate lyase, perform enzymatic hydrolysis, and obtain an enzymatic hydrolysis product; Step 4: The enzymatic hydrolysis product is separated by ultrafiltration, and the separated liquid is vacuum compressed and dried to obtain seaweed oligosaccharides.

2. The method for preparing seaweed oligosaccharides according to claim 1, characterized in that: The obtained seaweed oligosaccharide has a purity of 90%, and the remaining components are seaweed oligosaccharide, amino acids, trace elements and small molecule peptides.

3. The method for preparing seaweed oligosaccharides according to claim 1, characterized in that: The homogenizing pressure of the homogenizer in step 2 is 220-280Mpa, the temperature is controlled at 30-38°C, and the homogenizing time is 20-30 minutes.

4. The method for preparing seaweed oligosaccharides according to claim 1, characterized in that: The temperature of the enzymatic hydrolysis treatment in step 3 is 23-25° C., and the enzymatic hydrolysis time is 3-4 hours.

5. The method for preparing seaweed oligosaccharides according to claim 1, characterized in that: The pore size of the filter membrane used in the ultrafiltration separation in step 4 is 0.9-1.2nm.

6. The method for preparing seaweed oligosaccharides according to claim 1, characterized in that: The vacuum compression pressure in step 4 is 75-80Kpa and the temperature is 40-45°C.

7. The method for preparing seaweed oligosaccharides according to claim 1, characterized in that: The drying process in step 4 is carried out by microwave drying at a drying temperature of 75-80°C.