Distribution liquid flow distribution plate for large-scale chromatographic column, distribution assembly and chromatographic column thereof

Abstract

The invention provides a liquid flow distribution plate for a large-scale chromatographic column, a distribution assembly and a chromatographic column thereof, the liquid flow distribution plate comprises a distribution plate, a plurality of inwards concave distribution grooves are uniformly distributed on one surface of the plate body, and the cross section of the opening of each distribution groove is larger than the cross section of the bottom; the plate body is provided with a plurality of shunting holes, and the shunting holes correspond to the distribution grooves and penetrate through the plate body to communicate the distribution grooves with the other surface of the plate body; the other surface of the plate body adopts an open flow channel or a distribution flow channel; the distribution assembly comprises the distribution plate, and the distribution plate is placed in a groove of a column flange or a piston. One surface of the distribution plate with the open flow channel or the distribution flow channel is close to the column flange or the piston, and a screen plate is placed on one surface of the distribution plate with the distribution groove; the distribution plate is detachably and fixedly connected with the flange or the piston; the chromatographic column comprises the distribution plate and the distribution assembly; the method has the advantages of good distribution effect and high efficiency.

Description

Technical Field

[0001] The present invention relates to a chromatographic device for separation and purification in the field of biomedicine, and in particular to a distribution liquid flow distribution plate, a distribution component and a chromatographic column thereof for a large chromatographic column. Background Art

[0002] In the field of biomedicine, downstream chromatography columns are mainly used to effectively separate target products and remove impurities to obtain final products, such as biomacromolecule proteins, peptides, polysaccharides, ADCs, DNA and RNA, etc. The performance evaluation criteria of chromatography columns are continuity, stability, uniformity and sealing. Therefore, the distribution plate involved in the chromatography column plays an important role. The diverter plates used in the prior art currently have guide grooves and liquid guide holes, which can ensure the uniform distribution of the mobile phase. However, the disadvantages of the prior art are that the guide grooves are directly processed and formed on the guide plates, and the liquid passing through the mobile phase inlet directly acts on the sieve plate assembly. The structure on the sieve plate or the sieve plate assembly changes the liquid from a vertical flow direction to a horizontal flow direction. If the medium under the sieve plate is not compacted or has gaps, the effect of this diversion structure will be compromised. Summary of the invention

[0003] In order to overcome the above-mentioned deficiencies of the prior art, the present invention provides a distribution liquid flow distribution plate, a distribution assembly and a chromatography column for a large chromatography column, which can facilitate the installation of the plunger or flange and the distribution plate, and can enable the mobile phase to be quickly distributed on the cross section of the filler in the chromatography column to improve the column efficiency.

[0004] In order to achieve the above-mentioned purpose, the technical solution adopted by the present invention is:

[0005] In response to the above technical problems, the first aspect of the present invention discloses a distribution liquid flow distribution plate for a large chromatography column, comprising a plate body, a plurality of inwardly recessed distribution grooves evenly distributed on one side of the plate body, and the opening cross-section of the distribution grooves is larger than the bottom cross-section; the plate body is provided with a plurality of diversion holes, the diversion holes correspond to the distribution grooves, penetrate the plate body and connect the distribution grooves with the other side of the plate body; the other side of the plate body adopts an open flow channel or a distribution flow channel.

[0006] In some embodiments, the distribution grooves are evenly distributed on the plate in a concentric circle manner, and there are gaps between the distribution grooves.

[0007] In some embodiments, the longitudinal cross-section of the distribution groove is semicircular, inverted trapezoidal or triangular.

[0008] In some embodiments, the distribution groove adopts a conical structure.

[0009] In some embodiments, the distribution channel includes an inwardly recessed central groove, a plurality of concentric circular channels radiating outward from the central groove, the diversion holes are evenly distributed on the concentric circular channels, and the central groove and the concentric circular channels are connected via radial channels.

[0010] In some embodiments, the diversion hole is located at the central axis position of the bottom of the distribution groove.

[0011] In response to the above technical problems, the second aspect of the present invention discloses a distribution assembly for a large chromatography column, comprising the distribution plate described above, which is placed in a groove of a column flange or a piston; one side of the distribution plate having an open flow channel or a distribution flow channel is close to the column flange or the piston, and a sieve plate is placed on one side of the distribution plate having a distribution groove; the distribution plate is detachably fixedly connected to the flange or the piston.

[0012] In some embodiments, the distribution plate is fixedly connected to the column flange or piston by screws, the screws pass through the column flange or piston and the distribution plate, and a sealing ring is arranged on the screws between the distribution plate and the column flange or piston. A sealing ring is arranged between the distribution plate and the column flange or piston and / or between the distribution plate and the screen plate.

[0013] In some embodiments, the sum of the thicknesses of the distribution plate body 1 and the screen plate 13 is equal to the depth of the groove 9 .

[0014] In view of the above technical problems, a third aspect of the present disclosure provides a chromatography column, comprising the distribution plate or distribution assembly described above.

[0015] Beneficial effects of the present invention:

[0016] The distribution groove used in the present invention adopts a design in which the opening diameter is larger than the bottom diameter, which can make the liquid phase more evenly distributed into the chromatography column, achieving the best distribution effect. The open flow channel structure is simple and easy to install;

[0017] The distribution flow channel adopts a concentric radial flow channel, which can enable the liquid phase coming out of the central groove to quickly reach the distribution groove far away from the central groove, thereby improving the efficiency of liquid phase flow. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a structural diagram of one side of the distribution plate of the present invention.

[0019] Figure 2 This is a structural diagram of another side of embodiment 1 of the distribution plate of the present invention.

[0020] Figure 3 This is a structural diagram of another embodiment 2 of the distribution plate of the present invention.

[0021] Figure 4 It is a cross-sectional view of a partial structure of a distribution plate of the present invention.

[0022] Figure 5 This is a schematic diagram of the distribution tank structure of the present invention.

[0023] Figure 6 It is a schematic diagram of the structure of the distribution component of the present invention. DETAILED DESCRIPTION

[0024] The present invention will be further described below in conjunction with the accompanying drawings.

[0025] The technical content of the present invention is described below by specific embodiments. Those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments. Those skilled in the art can make various modifications and changes without departing from the spirit of the present invention.

[0026] Before describing the specific embodiments of the present disclosure in detail, some terms used in the present disclosure are first explained.

[0027] Unless otherwise defined below, the meaning of all technical terms and scientific terms used herein is intended to be the same as those commonly understood by those skilled in the art. Mentioning the technology used in this article is intended to refer to the technology commonly understood in the art, including those changes in technology that are obvious to those skilled in the art or the replacement of equivalent technology. Although it is believed that the following terms are well understood by those skilled in the art, the following definitions are still set forth to better explain the present invention. When a trade name appears in this article, it is intended to refer to the corresponding commodity. All patents, published patent applications and publications cited herein are incorporated herein by reference.

[0028] Unless otherwise indicated herein, multiple references such as "a", "the", include plural references. The expression "one or more" or "at least one" may mean 1, 2, 3, 4, 5, 6, 7, 8, 9 or more.

[0029] As used herein, the terms “connected”, “connected”, “coupled” or “coupled” and the like are not limited to direct connections but also include indirect connections.

[0030] As used herein, the "liquid phase" refers to biomolecules, including proteins, nucleic acids, lipids, carbohydrates, small nucleotides, amino acids and their derivatives.

[0031] The position relationship words "up", "down", "left", "right", "front", "back", etc. involved in this article are determined according to the layout direction of the drawings in the specification. They are only used to indicate relative position relationships. When the absolute position of the object being described changes, the relative position relationship may also change accordingly.

[0032] Reference Figure 1 The liquid distribution plate for a large-scale chromatography column comprises a plate body 1, a plurality of inwardly recessed distribution grooves 2 are evenly distributed on one side of the plate body 1, Figure 1 It can be seen that the distribution groove is circular, and a diversion hole 3 is provided at the center of each corresponding distribution groove 2. The diversion hole runs through both sides of the plate body 1. The distribution grooves 2 are arranged in a concentric circle, radiating outward and evenly distributed on the plate body 1, and there are gaps between each distribution groove 2.

[0033] Reference Figure 2 The other side of the distribution plate is shown, which adopts an open flow channel, that is, the other side of the plate body 1 is a smooth plate surface, on which corresponding diversion holes 3 and screw holes 4 for fixed connection are evenly arranged, and there are 12 screw holes 4.

[0034] Reference Figure 3 The other side of the distribution plate shown is a distribution channel, including an inwardly recessed central groove 5, the central groove 5 radiates multiple concentric circular channels 6 outward, 8 of which are provided here, the diversion holes 3 are evenly distributed on the concentric circular channels 6, the central groove 5 is connected to the concentric circular channels through radial channels 7, and 12 radial channels 7 are provided.

[0035] Reference Figure 4 As shown, it includes a body 1, the upper and lower surfaces of the body 1 are connected through a diversion hole 2, the diversion hole 2 is located at the central axis position of the distribution groove 2, the opening cross section of the distribution groove 2 is larger than the bottom cross section, and forms a conical structure.

[0036] Reference Figure 5 As shown, the distribution groove 2 has a longitudinal section of an inverted trapezoidal structure, such as a terrace or a truncated cone, or a cross section of a semicircular structure, such as a hemisphere, or a cone with a triangular longitudinal section.

[0037] Reference Figure 6, a distribution assembly for a large chromatography column, comprising the distribution plate described above, the distribution plate being placed in the groove 9 of the column flange or piston 8, the side of the distribution plate body 1 having an open flow channel or a distribution flow channel being close to the column flange or piston 8, and a screen plate 13 being placed on the side of the plate body 1 having a distribution groove 2; the sum of the thickness of the distribution plate body 1 and the screen plate 13 being equal to the depth of the groove 9. The plate body 1 and the column flange or piston 8 are fixedly connected by screws 10, and the screws 10 pass through the column flange or piston 8 to reach the screw holes 4 of the body 1. A sealing ring 11 is arranged between the plate body 1 and the column flange or piston 8 and between the body 1 and the screen plate 13; a sealing ring 2 12 is arranged on the screws 10 between the plate body 1 and the column flange or piston 8.

Claims

1. A liquid flow distribution plate for a large chromatography column, comprising a plate body, a plurality of inwardly recessed distribution grooves evenly distributed on one side of the plate body, wherein the opening cross section of the distribution grooves is larger than the bottom cross section; the plate body is provided with a plurality of diversion holes, wherein the diversion holes correspond to the distribution grooves and penetrate the plate body to connect the distribution grooves with the other side of the plate body; and the other side of the plate body adopts an open flow channel or a distribution flow channel.

2. The distribution plate according to claim 1, characterized in that The distribution grooves are evenly distributed on the plate body in a concentric circle manner, and there are gaps between the distribution grooves.

3. The distribution plate according to claim 1, characterized in that The longitudinal section of the distribution groove is semicircular, inverted trapezoidal or triangular.

4. The distribution plate according to claim 1, characterized in that The distribution trough adopts a conical structure.

5. The distribution plate according to claim 1, characterized in that The distribution channel comprises an inwardly concave central groove, a plurality of concentric circular channels radiating outward from the central groove, the diversion holes are evenly distributed on the concentric circular channels, and the central groove and the concentric circular channels are connected via radial channels.

6. The distribution plate according to claim 1, characterized in that The diversion hole is located at the central axis position of the bottom of the distribution groove.

7. A distribution assembly for a large chromatography column, characterized in that It includes the distribution plate described in claims 1-5, the distribution plate is placed in a groove of a column flange or a piston; the distribution plate has an open flow channel or a distribution flow channel on one side close to the column flange or the piston, and a screen plate is placed on the side of the distribution plate having a distribution groove; the distribution plate and the flange or the piston are detachably fixedly connected.

8. The distribution assembly for a large chromatography column according to claim 6, characterized in that: The distribution plate is fixedly connected to the column flange or the piston by screws, the screws pass through the column flange or the piston and the distribution plate, and sealing rings are arranged on the screws between the distribution plate and the column flange or the piston.

9. The distribution assembly for a large chromatography column according to claim 6, characterized in that: A sealing ring is arranged between the distribution plate and the column flange or the piston and / or between the distribution plate and the screen plate.

10. The distribution assembly for a large chromatography column according to claim 6, characterized in that: The sum of the thickness of the distribution plate body 1 and the screen plate 13 is equal to the depth of the groove 9 .

11. A chromatography column, characterized in that It comprises the distribution plate described in claims 1 to 6 or the distribution assembly described in claims 7 to 10.

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