Edible mushroom coffee for improving immunity as well as preparation method and application thereof

Abstract

The invention relates to the technical field of food, and particularly discloses edible mushroom coffee for improving immunity and a preparation method and application thereof, and the edible mushroom coffee is prepared from the following raw materials: coffee beans, lucid ganoderma, cordyceps militaris and poria cocos. The edible mushroom coffee has the beneficial effects that the ganoderma lucidum is used as a monarch drug, and the cordyceps militaris and the poria cocos are used as ministerial drugs; wherein the lucid ganoderma has the effects of tonifying qi, soothing the nerves, relieving cough and asthma, tonifying the lung and kidney, reinforcing qi, nourishing blood and the like, the cordyceps militaris has the effects of tonifying the lung and kidney, stopping bleeding, reducing phlegm, nourishing and protecting the liver and resisting fatigue, and the poria cocos has the effects of clearing damp, promoting diuresis, tonifying the spleen and nourishing the stomach and can harmonize all the medicines to achieve the effects of calming the heart, soothing the nerves, tonifying the kidney and tonifying the lung. The health-care functions of enhancing immunity, resisting bacteria, inducing diuresis and resisting tumors are realized; by adopting low-temperature extraction, effective components can be effectively prevented from being damaged, and by adopting a nanofiltration membrane separation technology, impurities in the product can be removed, and functional components in the product can be purified.

Description

Technical Field

[0001] The invention relates to the technical field of food, and in particular to edible fungus coffee for improving immunity, a preparation method thereof and applications thereof. Background Art

[0002] Traditional coffee drinks are mainly made by roasting and grinding coffee beans, and have functions such as refreshing and anti-fatigue. However, excessive intake of caffeine may have negative effects on the body, such as insomnia and anxiety. Therefore, it is necessary to develop a coffee drink that retains the coffee flavor and has health functions.

[0003] Edible fungi (such as Ganoderma lucidum, Cordyceps militaris, etc.) are rich in various bioactive ingredients and have various health benefits such as improving immunity, anti-oxidation, and anti-tumor. However, the taste of edible fungi eaten alone is poor, which limits its application range. Combining edible fungi with coffee can not only mask the odor of edible fungi, but also increase the nutritional value of coffee.

[0004] The Chinese patent with application number 202210297090.X discloses an edible fungus coffee companion and a preparation method, which belongs to the field of food processing technology. The present invention breaks the existing coffee companion formula, does not include hydrogenated vegetable oil, uses edible fungi, natural polysaccharides and milk pure natural ingredients as raw materials, and uses edible fungi to contain proteins, amino acids, minerals, vitamins, edible fungi polysaccharides, terpenes and other nutrients and biologically active ingredients to give edible fungi coffee companion health functions; natural polysaccharides are used to replace stabilizers, emulsifiers and anti-caking agents to encapsulate the effective ingredients of edible fungi, resist coagulation, slow release, avoid precipitation, and improve the brewing performance.

[0005] Although the edible fungus coffee companion provided in the above technical solution has certain health care effects, it has no therapeutic effect in improving immunity. Summary of the invention

[0006] The present invention is to solve the above problems and proposes an edible fungus coffee for improving immunity, a preparation method thereof and an application thereof.

[0007] The technical solution of the present invention is achieved in this way:

[0008] Edible fungus coffee for improving immunity is composed of the following raw materials: 30-40 parts of coffee beans, 20-30 parts of ganoderma lucidum, 15-25 parts of cordyceps militaris, and 10-20 parts of poria cocos. Ganoderma lucidum is used as the main drug, and cordyceps militaris and poria cocos are used as the auxiliary drugs; among them, ganoderma lucidum has the effects of replenishing qi and calming the mind, relieving cough and asthma, replenishing lungs and kidneys, replenishing qi and nourishing blood, etc. Cordyceps militaris has the effects of replenishing lungs and kidneys, stopping bleeding and resolving phlegm, nourishing and protecting the liver and resisting fatigue. Poria cocos not only has the effects of promoting water and removing dampness, strengthening the spleen and nourishing the stomach, but also can reconcile various drugs to achieve the effects of calming the mind and calming the mind, replenishing the kidney and lungs, and has the health care functions of enhancing immunity, antibacterial and diuretic, and anti-tumor.

[0009] Furthermore, it also includes 3-8 portions of Matsutake mushrooms, which have the effects of improving the body's immunity, anti-aging, replenishing qi and calming the mind. When used together with Ganoderma lucidum, it can further enhance immunity.

[0010] Furthermore, the invention also comprises 5-15 portions of edible fungus compositions, wherein the edible fungus compositions comprise one or more of shiitake mushrooms, straw mushrooms, black fungus, white fungus, hericium erinaceus, bamboo fungus, button mushroom, red mushroom, truffle, white mushroom, boletus, maitake mushroom, blazei matsutake, king oyster mushroom, morel, saddle mushroom, truffle, and enoki mushroom. Shiitake mushrooms can lower blood lipids, lower blood pressure, improve immunity, inhibit the growth of cancer cells, and enhance the body's anti-cancer effect; black fungus contains a high level of iron, which can prevent and treat iron deficiency anemia, improve the body's immune function, and increase the ability to resist diseases, and has a certain effect on preventing and fighting cancer. Effects: Tremella has the effects of strengthening the essence, nourishing the kidney, moistening the intestines, benefiting the stomach, replenishing qi, harmonizing blood, strengthening the heart, strengthening the body, nourishing the brain, refreshing, beautifying, rejuvenating the skin and prolonging life. It can enhance the body's immunity, has anti-tumor effects, and can also increase the tolerance of tumor patients to radiotherapy and chemotherapy; Hericium erinaceus can improve the body's immunity, protect the liver, lower blood lipids and blood sugar, and has anti-aging effects. Hericium erinaceus can also nourish the stomach, increase the barrier function of the gastric mucosa, and can be anti-inflammatory, anti-ulcer, and anti-tumor; Bamboo fungus can supplement the body with the nutrients it needs, thereby improving immunity and resisting disease The ability to prevent diseases; Oyster mushroom has the effects of lowering cholesterol, fighting cancer, preventing constipation and improving immunity; Red mushroom is rich in protein, which can enhance the body's immunity and improve the body's resistance to the outside world; Truffle, Boletus, Saddle mushroom and Truffle can enhance the body's resistance; White mushroom has anti-cancer and anti-cancer effects and can enhance the body's immune function; Grifola frondosa has the effects of invigorating qi and strengthening the spleen, replenishing deficiency and strengthening the body; Agaricus blazei can enhance the function of the mononuclear macrophage system, inhibit cell division and regulate the immune system response, thereby blocking the growth of interfering viruses and enhancing the body's own immune function Yes; King Oyster Mushroom has a good effect of strengthening the spleen and appetizing. Eating King Oyster Mushroom nourishes the spleen and stomach, which has a good effect of increasing appetite and promoting digestion for people with poor appetite and indigestion; Morel contains a variety of vitamins, as well as polysaccharides that inhibit tumors, and antibacterial and antiviral active ingredients. It has many functions such as enhancing the body's immunity, resisting fatigue, antiviral, and inhibiting tumors; Flammulina Enoki can inhibit the increase of blood lipids, lower cholesterol, and prevent and treat cardiovascular and cerebrovascular diseases; and Hericium erinaceus and Cordyceps militaris can assist edible fungus coffee in playing the health care role of improving immunity and resisting fatigue.

[0011] Furthermore, it also includes 3-8 portions of edible and medicinal Chinese medicine compositions, wherein the edible and medicinal Chinese medicine compositions include one or more of Dendrobium officinale, Astragalus, Polygonatum sibiricum, Ginseng, American Ginseng, Ophiopogon japonicus, Asparagus cochinchinensis, Rehmannia glutinosa, Cistanche deserticola, Codonopsis pilosula, and Cornus officinalis. Dendrobium candidum: sweet, slightly cold; enters the stomach and kidney meridians; functions and indications: nourishes the stomach and produces fluid, nourishes yin and clears heat; used for heat disease and fluid damage, dry mouth and thirst, insufficient stomach yin, poor appetite and dry retching, persistent deficiency heat after illness, excessive yin and fire, bone steaming and fatigue fever, blurred vision, and weak tendons and bones; Astragalus: sweet, slightly warm, enters the lung, spleen, liver, and kidney meridians; functions and indications: replenishes qi and produces yang, consolidates the exterior and stops sweating, promotes diuresis and reduces swelling, produces fluid and nourishes blood, relieves stagnation and relieves numbness, removes toxins and discharges pus, and heals sores and regenerates muscles; used for qi deficiency and fatigue, poor appetite and loose stools, sinking of qi in the middle, old diarrhea and prolapse of the anus, bloody stool and metrorrhagia, spontaneous sweating due to exterior deficiency, edema due to qi deficiency, internal heat and thirst, sallow complexion due to blood deficiency, hemiplegia, numbness due to numbness, carbuncle that is difficult to ulcerate, and long-term ulcer that does not heal; Polygonatum sibiricum: sweet, neutral; enters the spleen, lung, and kidney meridians; efficacy : Moisturizes the lungs and nourishes yin, tonifies the spleen and replenishes qi; Indications: dry cough caused by lung deficiency, chronic cough caused by overwork; soreness of waist and knees, premature graying of hair, dizziness and fatigue caused by kidney deficiency and essence deficiency, fatigue and weakness caused by qi deficiency, dry mouth and dry stool caused by yin deficiency; deficiency of both qi and yin, internal heat and thirst; Ginseng: sweet, slightly bitter, neutral in nature; enters the spleen, lung and heart meridians; Functions and Indications: greatly replenishes vital energy, restores pulse and consolidates collapse, replenishes the spleen and benefits the lungs, produces body fluid, and calms the mind; used for physical weakness and collapse, cold limbs and weak pulse, spleen deficiency, poor appetite and loose stools, shortness of breath and fatigue, lung deficiency, wheezing and coughing, thirst caused by body fluid loss, internal heat and thirst, long-term illness and weakness, palpitations and insomnia, impotence and cold uterus; heart failure, cardiogenic shock; American ginseng: bitter, slightly sweet, cold in nature; enters the heart, lung and kidney meridians; has the effects of replenishing qi and nourishing yin, clearing away heat and producing body fluid; Indications: deficiency of both qi and yin, deficiency of lung qi and lung yin Deficiency syndrome, fever, qi deficiency, loss of body fluid, thirst and polydipsia; American ginseng has the function of strengthening the central nervous system, protecting the cardiovascular system and improving immunity; American ginseng can reduce blood coagulation, inhibit platelet aggregation, resist atherosclerosis, promote red blood cell growth and increase hemoglobin; Ophiopogon japonicus: sweet, slightly bitter, slightly cold in nature; enters the heart, lung and stomach meridians, functions and indications: nourishing yin, promoting body fluid, moistening the lungs and relieving cough; treats dry cough due to lung dryness, thirst due to lack of body fluid, irritability, constipation and other symptoms; treats coronary heart disease and angina pectoris; Asparagus cochinchinensis: sweet, bitter, cold in nature; enters the lung meridian and kidney meridian; functions and indications: nourishing yin, moistening dryness, clearing the lungs and reducing fire; treats yin deficiency fever, cough and vomiting blood, pulmonary emphysema, lung abscess, sore throat, polydipsia and constipation; Rehmannia root: sweet, slightly bitter, slightly cold in nature; enters the lung meridian and kidney meridian; functions and indications: nourishing yin, moistening dryness, clearing the lungs and reducing fire; treats yin deficiency fever, cough and vomiting blood, pulmonary emphysema, lung abscess, sore throat, polydipsia and constipation; Warm; enters the liver and kidney meridians; functions and indications: nourishing yin and blood; treats yin deficiency and blood deficiency, waist and knee weakness, fatigue cough and bone steaming, spermatorrhea, metrorrhagia, irregular menstruation, thirst, frequent urination, deafness, blurred vision; Cistanche deserticola: sweet, salty, warm; enters the kidney and large intestine meridians; functions and indications: nourishing kidney yang, benefiting essence and blood, moistening intestines and relieving constipation; treats impotence due to kidney deficiency, spermatorrhea, cold pain in the waist and knees, dripping urine, hematuria, infertility due to uterine cold, leucorrhea, metrorrhagia, dry intestines and constipation; Codonopsis: sweet, neutral; enters the spleen and lung meridians; functions and indications: nourishing the middle and replenishing qi, promoting body fluid and nourishing blood; used for insufficient qi in the middle, poor appetite and loose stools, cough and shortness of breath, thirst due to loss of body fluid, sallow complexion due to blood deficiency, palpitations and dizziness; Cornus officinalis: sour, astringent, slightly warm; enters the liver and kidney meridians; functions and indications: nourishing the liver and kidney, astringing essence and consolidating deficiency;It is used for vertigo, tinnitus, sore waist and knees, impotence, spermatorrhea, enuresis, frequent urination, metrorrhagia, leukorrhea, sweating, exhaustion, internal heat and thirst; warms the liver and kidneys, eliminates all wind, stops menorrhagia, and treats frequent urination in the elderly; the Chinese medicine composition with the same origin of medicine and food has the effects of enhancing the body's immunity, nourishing and maintaining health, and regulating body functions. ;

[0012] Furthermore, the coffee bean is composed of the following raw materials in parts by weight: 30-40 parts of coffee beans, 20-30 parts of ganoderma lucidum, 15-25 parts of cordyceps militaris, 10-20 parts of tuckahoe, 3-8 parts of matsutake, 5-10 parts of hericium erinaceus, 5-10 parts of maitake mushroom, and 3-8 parts of dendrobium candidum. Ganoderma lucidum, cordyceps militaris, tuckahoe, maitake mushroom, hericium erinaceus and matsutake are complementary to each other, and coffee is used as the base material to achieve the remarkable effects of enhancing immunity, anti-aging, nourishing the lungs and kidneys, and calming the mind.

[0013] Furthermore, the invention is composed of the following raw materials in parts by weight: 35 parts of coffee beans, 20 parts of ganoderma lucidum, 15 parts of cordyceps militaris, 13 parts of tuckahoe, 4 parts of matsutake, 5 parts of hericium erinaceus, 5 parts of maitake mushroom, and 3 parts of dendrobium officinale.

[0014] A method for preparing edible fungus coffee for improving immunity comprises the following steps:

[0015] (1) Clean the raw materials and remove impurities and rotten and deteriorated raw materials;

[0016] (2) drying the cleaned raw materials and roasting the coffee beans;

[0017] (3) mixing and crushing the dried raw materials;

[0018] (4) extracting the crushed raw material with purified water at least once to obtain an extract;

[0019] (5) The extract is subjected to membrane separation to obtain intercepted liquid. The membrane separation technology can fully extract the effective ingredients of the raw materials without destroying the medicinal properties and ensure that the drink has a delicate taste;

[0020] (6) The separated intercepted liquid is concentrated, dried, crushed, and sieved to obtain edible fungus coffee.

[0021] Furthermore, in step (2), the raw material is dried at a temperature of 50-60° C. for 6-8 hours; the coffee beans are roasted at a temperature of 150-250° C. for 3-5 hours, thereby ensuring that the aroma of the coffee is fully released.

[0022] Furthermore, in step (3), the raw material is crushed into 5-20 meshes to ensure that the effective ingredients in the raw material are fully extracted.

[0023] Furthermore, in step (4), the extraction temperature is 50-60°C, and low-temperature extraction is performed to ensure that the nutrients are not destroyed. The extraction time is 5-10 hours to fully extract the effective ingredients in the raw materials.

[0024] Furthermore, the membrane separation technology in step (5) comprises the following steps:

[0025] 1) pre-treating the extract and filtering it through a 400-800 mesh sieve;

[0026] 2) Using nanofiltration membrane for filtration, the filtration conditions are: nanofiltration membrane space position 0.5-1nm, pressure 0.5-4MPa, temperature 20-40°C, and obtaining intercepted liquid after filtration through the nanofiltration membrane;

[0027] Furthermore, in the step (6), negative pressure vacuum concentration is adopted, and the concentration conditions are: pressure -0.08MPa, temperature 50-60°C; the concentrated liquid is dried at 50-60°C; and the concentrated liquid is sieved through an 80-mesh sieve.

[0028] The invention discloses an application of edible fungus coffee for improving immunity. The edible fungus coffee is added into drinking water or liquid beverage to obtain an edible fungus coffee beverage.

[0029] Furthermore, the addition amount of the edible fungus coffee is 5-10wt% of the mixed liquid.

[0030] Furthermore, the added amount of the edible fungus coffee is 8wt% of the mixed liquid.

[0031] The above technical scheme is adopted, and the beneficial effects of the present invention are as follows: the edible fungus coffee of the present application uses Ganoderma lucidum as the main medicine, and uses Cordyceps militaris and Poria cocos as the auxiliary medicines; among them, Ganoderma lucidum has the effects of replenishing qi and calming the mind, relieving cough and asthma, replenishing lungs and kidneys, replenishing qi and nourishing blood, etc. Cordyceps militaris has the effects of benefiting lungs and kidneys, stopping bleeding and resolving phlegm, nourishing liver and protecting liver, and resisting fatigue; Poria cocos not only has the effects of promoting diuresis and eliminating dampness, strengthening spleen and nourishing stomach, but also can reconcile various medicines to achieve the effects of calming the mind and calming the mind, replenishing kidney and benefiting lungs, and has the health care functions of enhancing immunity, antibacterial and diuretic, and anti-tumor; Matsutake has the effects of improving body immunity, anti-aging, replenishing qi and calming The combination of edible fungus composition, edible medicine and Chinese medicine composition with Cordyceps militaris can assist edible fungus coffee in improving immunity and anti-fatigue health care effects; low-temperature extraction can effectively ensure that the effective ingredients are not destroyed, and nanofiltration membrane separation technology can be used to remove impurities in the product and purify the effective ingredients in the product; the functional beverage made from the edible fungus coffee of the present invention has the functions of enhancing immunity, anti-aging, lowering blood sugar and blood lipids, and has a good effect of relieving neurasthenia, making people energetic. DETAILED DESCRIPTION

[0032] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] Embodiment 1:

[0034] The invention discloses edible fungus coffee for improving immunity, which is composed of the following raw materials in parts by weight: 35 parts of coffee beans, 20 parts of ganoderma lucidum, 15 parts of cordyceps militaris, 13 parts of tuckahoe, 7 parts of matsutake, 5 parts of hericium erinaceus and 5 parts of maitake mushroom.

[0035] A method for preparing edible fungus coffee for improving immunity comprises the following steps:

[0036] (1) Clean the raw materials and remove impurities and rotten and deteriorated raw materials;

[0037] (2) drying the cleaned raw materials at a drying temperature of 50° C. for 7 h, and roasting the coffee beans at a roasting temperature of 200° C. for 4 h;

[0038] (3) Mixing and crushing the dried raw materials into 20 mesh;

[0039] (4) extracting the crushed raw material with purified water at an extraction temperature of 60° C. for 8 h, and performing extraction twice to obtain an extract;

[0040] (5) subjecting the extract to membrane separation, wherein the membrane separation technology comprises the following steps:

[0041] 1) pre-treating the extract and filtering it through an 800-mesh sieve;

[0042] 2) Using nanofiltration membrane for filtration, the filtration conditions are: the nanofiltration membrane space is 1nm, the pressure is 2MPa, the temperature is 30°C, and the intercepted liquid is obtained after filtering through the nanofiltration membrane;

[0043] (6) The separated intercepted liquid is subjected to negative pressure vacuum concentration under the conditions of: pressure -0.08 MPa, temperature 50°C; the concentrated concentrated liquid is dried at a drying temperature of 60°C; then it is crushed by a grinder and finally passed through an 80-mesh sieve to obtain edible fungus coffee.

[0044] Embodiment 2:

[0045] The invention discloses edible fungus coffee for improving immunity, which is composed of the following raw materials in parts by weight: 30 parts of coffee beans, 25 parts of ganoderma lucidum, 20 parts of cordyceps militaris, 12 parts of tuckahoe, 3 parts of matsutake, 5 parts of hericium erinaceus and 5 parts of maitake mushroom.

[0046] Embodiment 3:

[0047] The invention discloses edible fungus coffee for improving immunity, which is composed of the following raw materials in parts by weight: 40 parts of coffee beans, 20 parts of ganoderma lucidum, 15 parts of cordyceps militaris, 12 parts of tuckahoe, 3 parts of matsutake, 5 parts of hericium erinaceus and 5 parts of maitake mushroom.

[0048] Embodiment 4:

[0049] The invention discloses edible fungus coffee for improving immunity, which is composed of the following raw materials in parts by weight: 40 parts of coffee beans, 20 parts of ganoderma lucidum, 15 parts of cordyceps militaris, 10 parts of tuckahoe, 3 parts of matsutake, 5 parts of hericium erinaceus, 4 parts of maitake mushroom and 3 parts of polygonatum.

[0050] Embodiment 5:

[0051] The invention discloses edible fungus coffee for improving immunity, which is composed of the following raw materials in parts by weight: 30 parts of coffee beans, 20 parts of ganoderma lucidum, 15 parts of cordyceps militaris, 12 parts of tuckahoe, 8 parts of matsutake, 5 parts of hericium erinaceus, 5 parts of maitake mushroom and 5 parts of dendrobium officinale.

[0052] Embodiment 6:

[0053] The invention discloses edible fungus coffee for improving immunity, which is composed of the following raw materials in parts by weight: 35 parts of coffee beans, 20 parts of ganoderma lucidum, 20 parts of cordyceps militaris, 15 parts of tuckahoe and 10 parts of maitake mushroom.

[0054] Comparative Example 1:

[0055] The invention discloses edible fungus coffee for improving immunity, which is composed of the following raw materials in parts by weight: 50 parts of coffee beans and 50 parts of ganoderma lucidum.

[0056] Comparative Example 2:

[0057] The invention discloses edible fungus coffee for improving immunity, which is composed of the following raw materials in parts by weight: 100 parts of coffee beans.

[0058] Comparative Example 3:

[0059] The invention discloses edible fungus coffee for improving immunity, which is composed of the following raw materials in parts by weight: 100 parts of ganoderma lucidum.

[0060] Comparative Example 4:

[0061] The invention discloses edible fungus coffee for improving immunity, which is composed of the following raw materials in parts by weight: 100 parts of Poria cocos.

[0062] Comparative Example 5:

[0063] The invention discloses edible fungus coffee for improving immunity, which is composed of the following raw materials in parts by weight: 100 parts of Hericium erinaceus.

[0064] The preparation methods of Examples 2-6 and Comparative Examples 1-5 are the same as that of Example 1.

[0065] The edible fungus coffee prepared in each embodiment and comparative example was added into the liquid in an amount of 8 wt % to obtain an edible fungus coffee functional beverage.

[0066] 1. Application Example 1:

[0067] 120 patients with hyperglycemia aged 51-65 were selected, including 65 females and 55 males, and all cases met the criteria of hyperglycemia (fasting blood glucose was above 7.0mmol / L). The above cases were randomly divided into 12 groups, one of which was a blank control group, and the remaining 11 groups drank the functional beverages prepared from the edible fungus coffee of each embodiment and comparative example, taking 150-200mL per day, and the average fasting blood glucose levels of each group of patients before and after taking it for 2 weeks were tested, and the test results are shown in Table 1.

[0068] project Fasting blood sugar before taking (mmol / L) Fasting blood glucose after 2 weeks of use (mmol / L) Blank control group 7.4 7.5 Example 1 7.3 6.2 Example 2 7.2 6.0 Example 3 7.1 6.1 Example 4 7.9 6.5 Example 5 7.8 6.1 Example 6 7.4 6.0 Comparative Example 1 7.5 7.0 Comparative Example 2 7.4 7.3 Comparative Example 3 7.3 7.4 Comparative Example 4 8.1 8.1 Comparative Example 5 7.7 7.9

[0069] Table 1

[0070] The results show that continuous consumption of the functional beverage prepared from the edible fungus coffee provided by the present invention can effectively lower blood sugar.

[0071] Application Example 2:

[0072] 150 patients with neurasthenia aged 45-65 were selected, including 75 males and 75 females, and all cases met the liver and kidney essence and blood deficiency type in the Chinese and Western medicine dialectical classification standard for neurosis. The above cases were randomly divided into 15 groups, one of which was a blank control group, and the other 14 groups drank the functional beverages prepared from the edible fungus coffee of each embodiment and comparative example, taking 150-200 mL per day, and the SCL-90 total score, sleep duration and TCM clinical symptom efficacy of each group of patients before and after taking it for 6 weeks were tested and diagnosed. 2. The results are shown in Table 2:

[0073] The results show that continuous consumption of the functional beverage prepared from the edible fungus coffee provided by the present invention can effectively improve the clinical symptoms of liver and kidney essence and blood deficiency, improve sleep quality, and treat neurasthenia.

[0074]

[0075] Table 2

[0076] 3. Results of the edible fungus coffee mouse experiment for improving immunity:

[0077] Objective: To evaluate the regulatory effect of edible fungus coffee (SYJKF) on the immune function of mice.

[0078] Experimental method: The ability of RAW264.7 cells to phagocytize neutral red was detected by colorimetry. In vivo experiments were conducted in a normal control group and a SYJKF-administered group; the normal control group was given normal saline, and the SYJKF 5, 10, 20 ml / kg (8 wt% of edible fungus coffee)-administered group was given for 28 days, after which the peripheral blood IgA and IgG levels were measured.

[0079] Test results:

[0080] Table 3 shows the effect of SYJKF on peripheral blood IgG and IgA antibodies in mice;

[0081] Table 4 shows the effect of SYJKF on the phagocytic function of RAW264.7 cells.

[0082] Group IgG (×103μg / ml) IgA (×104ng / ml) Blank control group 7.81±1.03 5.39±0.37 JJNS 5ml / kg 6.27±0.05* 4.07±0.23** JJNS10ml / kg 5.95±0.19** 3.55±0.27* JJNS20ml / kg 4.38±0.04* 3.08±0.32**

[0083] Table 3

[0084] Among them: *P<0.05, **P<0.01.

[0085] Group Absorbance value (A540nm) Neutral red phagocytosis index Blank control group 0.82±0.01 1.01±0.03 SYJKF 5ml / kg 0.86±0.04 1.1±0.12 SYJKF 10ml / kg 0.94±0.07* 1.21±0.06* SYJKF 20ml / kg 1.13±0.06* 1.33±0.08*

[0086] Table 4

[0087] Among them: *P<0.01.

[0088] From the above test data, it can be concluded that the results of the in vivo experiment showed that SYJKF significantly changed the secretion of IgG and IgA antibodies in the peripheral blood of mice. In the in vitro experiment, SYJKF can significantly improve the phagocytic ability of mouse RAW264.7 mononuclear macrophages to neutral red. According to the experimental judgment rules for enhancing immunity in the "Technical Specifications for Testing and Evaluation of Health Foods", the standard for judging the enhanced immune function is: if the results are positive in any two of the four aspects of cellular immune function, humoral immune function, mononuclear macrophage function, and NK cell activity, it can be confirmed that there is immune enhancement activity. Therefore, the edible fungus coffee has a significant regulatory effect on the immunity of mice.

[0089] 4. Raw material selection method:

[0090] 1. Ingredients selection:

[0091] Lingzhi is the dried fruiting body of the Polyporaceae fungus Ganoderma lucidum or Ganoderma rubrum. It has the effects of replenishing qi and calming the mind, relieving cough and asthma. It is mainly used to treat restlessness, insomnia, palpitations, cough, asthma, sputum, asthenia, etc. Therefore, this product uses Lingzhi as the main ingredient, coffee as the base material, and is supplemented with Cordyceps militaris, Poria cocos, Grifola frondosa, Hericium erinaceus, and Matsutake, in order to achieve the effect of improving the body's immunity.

[0092] 2. Product formula process design

[0093] 2.1 With Ganoderma lucidum as the main ingredient, a gradient test was designed to verify the effects of different proportions on product color, taste, flavor, etc.;

[0094] Ingredients ①Proportion / % ②Proportion / % ③Proportion / % ④Proportion / % ⑤Proportion of No. 5 / % Ganoderma 30 35 25 20 20 coffee 30 25 35 40 35 Cordyceps militaris 15 15 15 15 15 Poria 12 12 12 12 13 Grifola frondosa 5 5 5 5 5 Hericium erinaceus 5 5 5 5 5 Matsutake 3 3 3 3 7

[0095] The best solution was selected through test comparison. The product is brown in color, soft in taste, with a strong coffee aroma and mushroom flavor, and no special odor.

[0096] 2.2 In terms of product technology, by setting extraction parameters, verify the influence of different extraction processes on product leaching rate;

[0097] project Group ① Group ② Group ③ Group ④ Group ⑤ Group ⑥ Extraction time 8h / time 8h / time 5h / time 5h / time 8h / time 8h / time Number of extractions 1 time 2 times 2 times 1 time 1 time 2 times Extraction temperature 50-60℃ 50-60℃ 50-60℃ 50-60℃ 60-70℃ 60-70℃

[0098] The best solution was selected through experimental comparison, and the final product leaching rate was ≥15%.

[0099] 2.3 Comparative test of the amount of edible fungus coffee added to the product to verify the effect of different addition amounts on the finished product during the shelf life;

[0100]

[0101] Through accelerated tests, we verify the impact of edible fungus coffee on the stability of the finished product during the shelf life, and finally select the optimal solution to determine factors such as the product's color, taste, and effective ingredient consumption.

[0102] 3. Through the above scheme, experiments were carried out according to different ratios, different processes and different addition amounts, and finally the optimal formula and process of the product were determined. The optimal formula is 35 parts of coffee beans, 20 parts of Ganoderma lucidum, 15 parts of Cordyceps militaris, 13 parts of Poria cocos, 7 parts of Matsutake, 5 parts of Hericium erinaceus, and 5 parts of Grifola frondosa.

[0103] Technologies not described in detail herein are prior art.

[0104] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An edible fungus coffee for improving immunity, characterized in that: The invention is composed of the following raw materials: 30-40 parts of coffee beans, 20-30 parts of ganoderma lucidum, 15-25 parts of cordyceps militaris and 10-20 parts of tuckahoe.

2. The edible fungus coffee for improving immunity according to claim 1, characterized in that: Also includes 3-8 servings of Matsutake mushrooms.

3. The edible fungus coffee for improving immunity according to claim 2, characterized in that: It also includes 5-15 portions of edible fungus compositions, which include one or more of shiitake mushrooms, straw mushrooms, black fungus, white fungus, hericium erinaceus, bamboo fungus, button mushroom, red mushroom, truffle, white mushroom, boletus, maitake mushroom, blazei murill, king oyster mushroom, morel, saddle mushroom, truffle, and enoki mushroom.

4. The edible fungus coffee for improving immunity according to claim 3, characterized in that: It also includes 3-8 portions of edible and medicinal Chinese medicine compositions, wherein the edible and medicinal Chinese medicine compositions include one or more of Dendrobium officinale, Astragalus, Polygonatum sibiricum, ginseng, American ginseng, Ophiopogon japonicus, Asparagus cochinchinensis, Rehmannia glutinosa, Cistanche deserticola, Codonopsis pilosula, and Cornus officinalis.

5. The edible fungus coffee for improving immunity according to claim 4, characterized in that: The invention is composed of the following raw materials in parts by weight: 30-40 parts of coffee beans, 20-30 parts of ganoderma lucidum, 15-25 parts of cordyceps militaris, 10-20 parts of tuckahoe, 3-8 parts of matsutake, 5-10 parts of hericium erinaceus, 5-10 parts of maitake mushroom and 3-8 parts of dendrobium officinale.

6. The edible fungus coffee for improving immunity according to claim 5, characterized in that: The invention is composed of the following raw materials in parts by weight: 35 parts of coffee beans, 20 parts of ganoderma lucidum, 15 parts of cordyceps militaris, 13 parts of tuckahoe, 4 parts of matsutake, 5 parts of hericium erinaceus, 5 parts of maitake mushroom and 3 parts of dendrobium officinale.

7. A method for preparing edible fungus coffee for improving immunity, characterized in that: The following steps are involved: (1) Clean the raw materials and remove impurities and rotten and deteriorated raw materials; (2) drying the cleaned raw materials at a temperature of 50-60° C. for 6-8 hours, and roasting the coffee beans at a temperature of 150-250° C. for 3-5 hours; (3) Mixing and crushing the dried raw materials into 5-20 mesh; (4) extracting the crushed raw material with purified water at a temperature of 50-60° C. for 5-10 h, performing the extraction at least once, and obtaining an extract; (5) subjecting the extract to membrane separation, wherein the membrane separation technology comprises the following steps: 1) pre-treating the extract and filtering it through a 400-800 mesh sieve; 2) Using nanofiltration membrane for filtration, the filtration conditions are: nanofiltration membrane space position 0.5-1nm, pressure 0.5-4MPa, temperature 20-40°C, and obtaining intercepted liquid after filtration through the nanofiltration membrane; (6) The separated intercepted liquid is subjected to negative pressure vacuum concentration under the conditions of: pressure -0.08MPa, temperature 50-60°C; the concentrated concentrated liquid is dried at a drying temperature of 50-60°C; then it is crushed by a grinder and finally passed through an 80-mesh sieve to obtain edible fungus coffee.

8. An application of edible fungus coffee for improving immunity, characterized in that: The edible fungus coffee is added into drinking water or liquid beverage to obtain an edible fungus coffee beverage.

9. The use of edible fungus coffee for improving immunity according to claim 8, characterized in that: The added amount of the edible fungus coffee is 5-10wt% of the mixed liquid.

10. The use of edible fungus coffee for improving immunity according to claim 9, characterized in that: The added amount of the edible fungus coffee is 8wt% of the mixed liquid.

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