Anti-oxidation composition based on plant extract and preparation process of anti-oxidation composition

Abstract

The invention relates to the technical field of cosmetics, in particular to an antioxidant composition based on plant extracts and a preparation process of the antioxidant composition. The antioxidant composition is prepared by compounding the following raw materials in parts by mass: 6.5 to 7.5 parts of mulberry root extract, 2.5 to 3.5 parts of guava extract, 0.5 to 1 part of glycerol, 8.5 to 11 parts of butanediol and 45 to 55 parts of purified water.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, in particular to an antioxidant composition based on plant extracts and a preparation process thereof. Background Art

[0002] With people's increasing attention to health and beauty, the demand for whitening skin care products continues to increase. Plant extracts have gradually become a research hotspot in the field of whitening skin care due to their rich bioactive ingredients and relatively few side effects. In the prior art, most antioxidant whitening skin care products rely on substances such as vitamin C. Although they have obvious effects, they are prone to adverse effects such as irritation and swelling for sensitive people such as those with intolerance; at the same time, they have the disadvantages of poor ingredient stability, low absorption efficiency, and poor safety. Therefore, researchers proposed the use of plant extracts to increase antioxidant properties and reduce irritation. However, the existing formulas of plant extracts have the disadvantages of limited antioxidant effects and low inhibition of tyrosinase activity.

[0003] In summary, it is of great significance to prepare an antioxidant composition based on plant extracts. Summary of the invention

[0004] The object of the present invention is to provide an antioxidant composition based on plant extracts and a preparation process thereof, so as to solve the problems raised in the prior art.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A preparation process of an antioxidant composition based on plant extracts: the antioxidant composition is compounded according to the following parts by mass, including: 6.5 to 7.5 parts of mulberry root extract, 2.5 to 3.5 parts of guava extract, 0.5 to 1 part of glycerol, 8.5 to 11 parts of butylene glycol, and 45 to 55 parts of purified water.

[0007] In the scheme, adding mulberry root extract and guava extract in a specific ratio can show the best antioxidant and tyrosinase inhibition effects. This ratio not only ensures the full release and complementarity of the active ingredients of the two, but also avoids skin irritation or adverse reactions that may be caused by excessive concentration, making the skin care effect more gentle and significant. Mulberry root extract and guava extract can form a synergistic effect in antioxidant and tyrosinase inhibition when compounded, mainly because the active ingredients contained in the two can complement each other and enhance each other's biological activity. Mulberry root extract contains a variety of antioxidant ingredients, and guava extract, especially root extract, has been proven to have strong antioxidant, hypoglycemic and tyrosinase inhibition activities. The combination of the two, by complementing each other's antioxidant ingredients, forms a powerful antioxidant network, effectively protects the skin from oxidative stress and maintains a healthy and youthful skin.

[0008] Among them, mulberry root extract is rich in sugars, organic acids, lipids, vitamins, tannic acid, anthocyanins, minerals, etc. It has good antioxidant properties and can inhibit the generation and activity of free radicals, thereby delaying skin aging. Mulberry root extract promotes the activation of luciferase and has anti-inflammatory effects, which can help reduce skin inflammation and relieve skin discomfort; guava extract shows strong inhibition of B-16 melanocyte activity, which means that it can effectively reduce the production of melanin, thereby playing a role in whitening and removing spots. Guava extract has a good anti-lipid peroxidation effect and can delay skin aging. At the same time, it is also a natural antioxidant that can protect the skin from damage by free radicals. Guava extract's inhibition of xanthine oxidase shows anti-inflammatory and astringent effects, which helps to tighten pores, reduce skin oil secretion, and make the skin tighter and smoother.

[0009] More optimally, the preparation process of the mulberry root extract comprises the following steps:

[0010] S1-1: crushing mulberry root to obtain fine powder, adding ethanol, thermally extracting at 65-68° C. for 10-12 hours, cooling and separating to obtain mulberry root filtrate A and filter residue;

[0011] S1-2: adding the filter residue to ethanol again, adding cellulase and pectinase, extracting at room temperature for 12 to 24 hours, filtering, and obtaining mulberry root filtrate B;

[0012] S1-3: Evenly mix the mulberry root filtrate A and the mulberry root filtrate B, and sequentially perform reduced pressure distillation, concentration, and freeze-drying to obtain a mulberry root extract.

[0013] More optimally, the volume ratio of fine powder to ethanol in the mulberry root filtrate A is 1:7-9.

[0014] More optimally, the mass ratio of the filter residue, ethanol, cellulase and pectinase in the mulberry root filtrate B is 1:5:0.05-0.06:0.02-0.03.

[0015] More optimally, the preparation process of the guava extract comprises the following steps:

[0016] S2-1: adding guava leaves to a low eutectic solvent and an extractant, crushing, ultrasonically treating at 30-38° C., filtering, and obtaining a supernatant and a filter residue;

[0017] S2-2: Adding the macroporous resin and the supernatant to the chromatography column in sequence, eluting with deionized water and 75% to 85% ethanol aqueous solution to obtain an eluate; concentrating the eluate, and freeze-drying it to obtain the guava extract.

[0018] More optimally, in the low eutectic solvent, glucose is used as a hydrogen donor and choline chloride is used as a hydrogen acceptor; the low eutectic solvent comprises glucose and choline chloride in a mass ratio of 1-1.2:1-1.2.

[0019] More optimally, the mass ratio of the guava leaves, the low eutectic solvent, and the auxiliary extraction agent is 1:5 to 7:0.3.

[0020] Among them, by using a low eutectic solvent as an extraction medium, the cell wall of guava cells can be effectively destroyed, promoting the release of active ingredients such as guava polyphenols. This solvent system has a low eutectic point and can accelerate solute diffusion through ultrasonic treatment under mild conditions to improve extraction efficiency. Mild extraction conditions (low temperature, ultrasound) help reduce the degradation of heat-sensitive components such as guava polyphenols and protect their biological activity. At the same time, low eutectic solvents usually have good biocompatibility and degradability, reduce pollution to the environment, and the extraction process is relatively simple, which is convenient for subsequent production.

[0021] More optimally, the preparation method of the auxiliary extraction agent comprises the following steps:

[0022] Cinnamic acid and p-toluenesulfonic acid are added to an ethanol aqueous solution, reacted at 35-45° C. for 2-4 hours, cooled, filtered, and purified to obtain an auxiliary extraction agent.

[0023] More optimally, the auxiliary extraction agent comprises the following raw materials in parts by weight: 5 to 6 parts of cinnamic acid, 0.5 to 1 part of p-toluenesulfonic acid, and 30 to 40 parts of ethanol aqueous solution.

[0024] Among them, the polyphenol compounds and alcohols are subjected to an esterification reaction. Since the esterified structure has better solubility, it can help the polyphenol compounds in guava leaves to dissolve more effectively in the low eutectic solvent. Through reasonable esterification modification, the biological activity of polyphenols may be maintained or even enhanced, which is of great significance for the biological activity of subsequent extracts. Components such as cinnamic acid and p-toluenesulfonic acid in the co-extractant can improve the solubility of these active ingredients in ethanol aqueous solution by interacting with the active ingredients in guava leaves, which helps to release and extract the target active ingredients from guava leaves more effectively. Its specific chemical structure and properties can more effectively combine with the active ingredients in guava leaves, thereby accelerating the extraction process. The use of co-extractants can also reduce the time and energy required for extraction and improve overall production efficiency. In addition, the components in the co-extractant have a stabilizing effect, which can protect the active ingredients in guava leaves from being destroyed or degraded during the extraction process. This makes the final guava extract have higher activity and purity. The presence of the co-extractant promotes the release of polyphenolic compounds from guava leaves into the solvent, thereby improving the extraction efficiency and also helps to reduce the interference of impurities and unwanted components, thereby improving the purity of the final extract.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] 1. The solution uses a specific method to extract and prepare mulberry root extract and guava extract, and first defines the ratio to achieve the best antioxidant and tyrosinase inhibition effects, ensure the complementary release of active ingredients, avoid high-concentration stimulation, and gently enhance skin care efficacy. The two work synergistically to enhance antioxidant capacity, protect the skin from oxidative damage, and maintain healthy and youthful skin.

[0027] 2. The scheme uses a low eutectic solvent as the extraction medium, which can effectively destroy the guava cell wall, promote the release of active ingredients, and improve the extraction efficiency. Mild conditions (low temperature, ultrasound) protect heat-sensitive components, and the solvent has good biocompatibility and is degradable, which reduces environmental pollution and simplifies the extraction process.

[0028] 3. The esterification reaction of polyphenol compounds with alcohols in the scheme improves their solubility, which helps guava leaf polyphenols to dissolve more effectively in deep eutectic solvents. Esterification modification may maintain or enhance the biological activity of polyphenols, and the extractant can improve the extraction efficiency. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] In the following scheme, the specific steps of preparing the auxiliary extraction agent are as follows: adding 5.5 parts of cinnamic acid and 0.8 parts of p-toluenesulfonic acid to 35 parts of ethanol aqueous solution, reacting at 40° C. for 3 hours, cooling, filtering and purifying to obtain the auxiliary extraction agent.

[0031] The deep eutectic solvent includes glucose and choline chloride in a mass ratio of 1:1.

[0032] The mass ratio of guava leaves, low eutectic solvent and auxiliary extraction agent is 1:6:0.3.

[0033] Example 1: A process for preparing an antioxidant composition based on plant extracts, comprising the following steps:

[0034] Step 1: Grind the mulberry root to obtain fine powder, add ethanol, the volume ratio of the fine powder to ethanol is 1:8, heat extract at 65°C for 12 hours, cool and separate to obtain mulberry root filtrate A and filter residue; add the filter residue to ethanol again, add cellulase and pectinase; the mass ratio of the filter residue, ethanol, cellulase and pectinase is 1:5:0.05:0.03; extract at room temperature for 24 hours, filter and obtain mulberry root filtrate B; mix mulberry root filtrate A and mulberry root filtrate B evenly, distill under reduced pressure, concentrate and freeze-dry in sequence to obtain mulberry root extract;

[0035] Step 2: adding guava leaves to a low eutectic solvent and an extractant, crushing, ultrasonically treating at 34° C., filtering to obtain a supernatant and a filter residue; adding a macroporous resin and a supernatant to a chromatography column in sequence, eluting with deionized water and an 80% ethanol-containing aqueous solution to obtain an eluent; concentrating the eluent, freeze-drying, and obtaining a guava extract;

[0036] Step 3: Evenly mix 7 parts of mulberry root extract, 3 parts of guava extract, 0.8 parts of glycerin, 10 parts of butylene glycol and 50 parts of purified water to obtain an antioxidant composition.

[0037] Example 2: A process for preparing an antioxidant composition based on plant extracts, comprising the following steps:

[0038] Step 1: Grind the mulberry root to obtain fine powder, add ethanol, the volume ratio of the fine powder to ethanol is 1:8, heat extract at 65°C for 12 hours, cool and separate to obtain mulberry root filtrate A and filter residue; add the filter residue to ethanol again, add cellulase and pectinase; the mass ratio of the filter residue, ethanol, cellulase and pectinase is 1:5:0.05:0.03; extract at room temperature for 24 hours, filter and obtain mulberry root filtrate B; mix mulberry root filtrate A and mulberry root filtrate B evenly, distill under reduced pressure, concentrate and freeze-dry in sequence to obtain mulberry root extract;

[0039] Step 2: adding guava leaves to a low eutectic solvent and an extractant, crushing, ultrasonically treating at 34° C., filtering to obtain a supernatant and a filter residue; adding a macroporous resin and a supernatant to a chromatography column in sequence, eluting with deionized water and an 80% ethanol-containing aqueous solution to obtain an eluent; concentrating the eluent, freeze-drying, and obtaining a guava extract;

[0040] Step 3: 6.5 parts of mulberry root extract, 2.5 parts of guava extract, 0.5 parts of glycerin, 8.5 parts of butylene glycol, and 45 parts of purified water are mixed uniformly to obtain an antioxidant composition.

[0041] Example 3: A process for preparing an antioxidant composition based on plant extracts, comprising the following steps:

[0042] Step 1: Grind the mulberry root to obtain fine powder, add ethanol, the volume ratio of the fine powder to ethanol is 1:8, heat extract at 65°C for 12 hours, cool and separate to obtain mulberry root filtrate A and filter residue; add the filter residue to ethanol again, add cellulase and pectinase; the mass ratio of the filter residue, ethanol, cellulase and pectinase is 1:5:0.05:0.03; extract at room temperature for 24 hours, filter and obtain mulberry root filtrate B; mix mulberry root filtrate A and mulberry root filtrate B evenly, distill under reduced pressure, concentrate and freeze-dry in sequence to obtain mulberry root extract;

[0043] Step 2: adding guava leaves to a low eutectic solvent and an extractant, crushing, ultrasonically treating at 34° C., filtering to obtain a supernatant and a filter residue; adding a macroporous resin and a supernatant to a chromatography column in sequence, eluting with deionized water and an 80% ethanol-containing aqueous solution to obtain an eluent; concentrating the eluent, freeze-drying, and obtaining a guava extract;

[0044] Step 3: 7.5 parts of mulberry root extract, 3.5 parts of guava extract, 1 part of glycerin, 11 parts of butylene glycol, and 55 parts of purified water are mixed uniformly to obtain an antioxidant composition.

[0045] Comparative Example 1: Based on Example 1, a single mulberry root extract, the rest of the process remains unchanged, and the specific modified steps are as follows:

[0046] Step 1: Grind the mulberry root to obtain fine powder, add ethanol, the volume ratio of the fine powder to ethanol is 1:8, heat extract at 65°C for 12 hours, cool and separate to obtain mulberry root filtrate A and filter residue; add the filter residue to ethanol again, add cellulase and pectinase; the mass ratio of the filter residue, ethanol, cellulase and pectinase is 1:5:0.05:0.03; extract at room temperature for 24 hours, filter and obtain mulberry root filtrate B; mix mulberry root filtrate A and mulberry root filtrate B evenly, distill under reduced pressure, concentrate and freeze-dry in sequence to obtain mulberry root extract;

[0047] Step 2: adding guava leaves to a low eutectic solvent and an extractant, crushing, ultrasonically treating at 34° C., filtering to obtain a supernatant and a filter residue; adding a macroporous resin and a supernatant to a chromatography column in sequence, eluting with deionized water and an 80% ethanol-containing aqueous solution to obtain an eluent; concentrating the eluent, freeze-drying, and obtaining a guava extract;

[0048] Step 3: Evenly mix 10 parts of mulberry root extract, 0.8 parts of glycerin, 10 parts of butylene glycol and 50 parts of purified water to obtain an antioxidant composition.

[0049] Comparative Example 2: Based on Example 1, the ratio of the two components was adjusted, and the other processes remained unchanged. The specific modified steps were:

[0050] Step 1: Grind the mulberry root to obtain fine powder, add ethanol, the volume ratio of the fine powder to ethanol is 1:8, heat extract at 65°C for 12 hours, cool and separate to obtain mulberry root filtrate A and filter residue; add the filter residue to ethanol again, add cellulase and pectinase; the mass ratio of the filter residue, ethanol, cellulase and pectinase is 1:5:0.05:0.03; extract at room temperature for 24 hours, filter and obtain mulberry root filtrate B; mix mulberry root filtrate A and mulberry root filtrate B evenly, distill under reduced pressure, concentrate and freeze-dry in sequence to obtain mulberry root extract;

[0051] Step 2: adding guava leaves to a low eutectic solvent and an extractant, crushing, ultrasonically treating at 34° C., filtering to obtain a supernatant and a filter residue; adding a macroporous resin and a supernatant to a chromatography column in sequence, eluting with deionized water and an 80% ethanol-containing aqueous solution to obtain an eluent; concentrating the eluent, freeze-drying, and obtaining a guava extract;

[0052] Step 3: Evenly mix 5 parts of mulberry root extract, 5 parts of guava extract, 0.8 parts of glycerin, 10 parts of butylene glycol, and 50 parts of purified water to obtain an antioxidant composition.

[0053] Comparative Example 3: Based on Example 1, pectinase was not added, and the other processes remained unchanged. The specific modified steps were:

[0054] Step 1: crush the mulberry root to obtain fine powder, add ethanol, the volume ratio of the fine powder to ethanol is 1:8, heat extract at 65°C for 12 hours, cool and separate to obtain mulberry root filtrate A and filter residue; add the filter residue to ethanol again, add cellulase; the mass ratio of the filter residue, ethanol and cellulase is 1:5:0.08; extract at room temperature for 24 hours, filter and obtain mulberry root filtrate B; mix mulberry root filtrate A and mulberry root filtrate B evenly, distill under reduced pressure, concentrate and freeze-dry in sequence to obtain mulberry root extract;

[0055] Step 2: adding guava leaves to a low eutectic solvent and an extractant, crushing, ultrasonically treating at 34° C., filtering to obtain a supernatant and a filter residue; adding a macroporous resin and a supernatant to a chromatography column in sequence, eluting with deionized water and an 80% ethanol-containing aqueous solution to obtain an eluent; concentrating the eluent, freeze-drying, and obtaining a guava extract;

[0056] Step 3: Evenly mix 7 parts of mulberry root extract, 3 parts of guava extract, 0.8 parts of glycerin, 10 parts of butylene glycol and 50 parts of purified water to obtain an antioxidant composition.

[0057] Comparative Example 4: Based on Example 1, no auxiliary extraction agent was added, and the other processes remained unchanged. The specific modified steps were:

[0058] Step 1: Grind the mulberry root to obtain fine powder, add ethanol, the volume ratio of the fine powder to ethanol is 1:8, heat extract at 65°C for 12 hours, cool and separate to obtain mulberry root filtrate A and filter residue; add the filter residue to ethanol again, add cellulase and pectinase; the mass ratio of the filter residue, ethanol, cellulase and pectinase is 1:5:0.05:0.03; extract at room temperature for 24 hours, filter and obtain mulberry root filtrate B; mix mulberry root filtrate A and mulberry root filtrate B evenly, distill under reduced pressure, concentrate and freeze-dry in sequence to obtain mulberry root extract;

[0059] Step 2: adding guava leaves to a low eutectic solvent, wherein the mass ratio of guava leaves to the low eutectic solvent is 1:6, crushing, ultrasonically treating at 34°C, filtering to obtain a supernatant and a filter residue; adding a macroporous resin and a supernatant to a chromatography column in sequence, eluting with deionized water and an 80% ethanol-containing aqueous solution to obtain an eluent; concentrating the eluent, and freeze-drying to obtain a guava extract;

[0060] Step 3: Evenly mix 7 parts of mulberry root extract, 3 parts of guava extract, 0.8 parts of glycerin, 10 parts of butylene glycol and 50 parts of purified water to obtain an antioxidant composition.

[0061] Detection experiment: The relevant properties of an antioxidant composition based on plant extracts prepared in Examples 1 to 3 and Comparative Examples 1 to 4 were detected; the process is as follows: The antioxidant composition based on plant extracts prepared in Examples 1 to 3 and Comparative Examples 1 to 4 was subjected to tyrosinase inhibition rate test and free radical scavenging rate test; specifically: (1) Tyrosinase inhibition rate test: The antioxidant composition prepared in Examples 1 to 3 and Comparative Examples 1 to 4 was referred to the HMC-WI-032 experimental method, the absorbance of the sample solution was measured at 475 nm, and the tyrosinase inhibition rate was calculated; (2) Free radical scavenging rate test: The antioxidant composition prepared in Examples 1 to 3 and Comparative Examples 1 to 4 was referred to the HMC-WI-030DPPH experimental method, the absorbance of the sample solution was measured at 517 nm, and the free radical scavenging rate was calculated.

[0062] The obtained data are shown in the following table:

[0063]

[0064] Table 1

[0065] Result analysis: According to the data analysis in Table 1, it can be seen that the present invention achieves the best antioxidant and tyrosinase inhibition effects by adding mulberry root extract and guava extract in a specific ratio, ensures the complementary release of active ingredients, avoids high concentration stimulation, and gently improves the skin care effect. The two work synergistically to enhance the antioxidant capacity, protect the skin from oxidative damage, and maintain healthy and young skin; using a low eutectic solvent as an extraction medium can effectively destroy the guava cell wall, promote the release of active ingredients, and improve the extraction efficiency. Mild conditions (low temperature, ultrasound) protect heat-sensitive components, and the solvent has good biocompatibility and is degradable, reduces environmental pollution, and simplifies the extraction process. The esterification reaction of polyphenol compounds with alcohols improves their solubility, which helps guava leaf polyphenols to dissolve more effectively in low eutectic solvents. Esterification modification may maintain or enhance the biological activity of polyphenols, and the auxiliary extractant can improve the extraction efficiency.

[0066] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.

Claims

1. A process for preparing an antioxidant composition based on plant extracts, characterized in that: The antioxidant composition is compounded according to the following parts by mass, including: 6.5-7.5 parts of mulberry root extract, 2.5-3.5 parts of guava extract, 0.5-1 part of glycerol, 8.5-11 parts of butylene glycol, and 45-55 parts of purified water.

2. The process for preparing an antioxidant composition based on plant extracts according to claim 1, characterized in that: The preparation process of the mulberry root extract comprises the following steps: S1-1: crushing mulberry root to obtain fine powder, adding ethanol, thermally extracting at 65-68° C. for 10-12 hours, cooling and separating to obtain mulberry root filtrate A and filter residue; S1-2: adding the filter residue to ethanol again, adding cellulase and pectinase, extracting at room temperature for 12 to 24 hours, filtering, and obtaining mulberry root filtrate B; S1-3: Evenly mix the mulberry root filtrate A and the mulberry root filtrate B, and sequentially perform reduced pressure distillation, concentration, and freeze-drying to obtain a mulberry root extract.

3. The process for preparing an antioxidant composition based on plant extracts according to claim 2, characterized in that: The volume ratio of fine powder to ethanol in the mulberry root filtrate A is 1:7-9.

4. The process for preparing the antioxidant composition based on plant extracts according to claim 2, characterized in that: The mass ratio of the filter residue, ethanol, cellulase and pectinase in the mulberry root filtrate B is 1:5:0.05-0.06:0.02-0.

03.

5. The process for preparing the antioxidant composition based on plant extracts according to claim 1, characterized in that: The preparation process of the guava extract comprises the following steps: S2-1: adding guava leaves to a low eutectic solvent and an extractant, crushing, ultrasonically treating at 30-38° C., filtering, and obtaining a supernatant and a filter residue; S2-2: Adding the macroporous resin and the supernatant to the chromatography column in sequence, eluting with deionized water and 75% to 85% ethanol aqueous solution to obtain an eluate; concentrating the eluate, and freeze-drying it to obtain the guava extract.

6. The process for preparing the antioxidant composition based on plant extracts according to claim 5, characterized in that: In the low eutectic solvent, glucose is used as a hydrogen donor and choline chloride is used as a hydrogen acceptor; the low eutectic solvent comprises glucose and choline chloride in a mass ratio of 1-1.2:1-1.

2.

7. The process for preparing an antioxidant composition based on plant extracts according to claim 5, characterized in that: The mass ratio of the guava leaves, the low eutectic solvent and the auxiliary extraction agent is 1:5 to 7:0.

3.

8. The process for preparing the antioxidant composition based on plant extracts according to claim 5, characterized in that: The preparation method of the auxiliary extraction agent comprises the following steps: Cinnamic acid and p-toluenesulfonic acid are added to an ethanol aqueous solution, reacted at 35-45° C. for 2-4 hours, cooled, filtered, and purified to obtain an auxiliary extraction agent.

9. The process for preparing the antioxidant composition based on plant extracts according to claim 8, characterized in that: The auxiliary extraction agent comprises the following raw materials in parts by weight: 5 to 6 parts of cinnamic acid, 0.5 to 1 part of p-toluenesulfonic acid, and 30 to 40 parts of ethanol aqueous solution.

10. An antioxidant composition prepared according to the process for preparing an antioxidant composition based on plant extracts according to any one of claims 1 to 9.

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