Whitening and spot-fading composition containing resveratrol

Abstract

The invention discloses a whitening and spot-fading composition containing resveratrol, and belongs to the field of cosmetics. The composition consists of resveratrol, a scutellaria baicalensis extract, a honeysuckle flower extract, a purslane extract, a achillea millefolium extract and a cassia occidentalis seed extract. According to the composition provided by the invention, all the raw materials are compounded for use and have a synergistic effect, so that excellent whitening and spot-fading effects are obtained.

Description

Technical Field

[0001] The present application relates to the field of cosmetics, and in particular to a whitening and freckle-removing composition containing resveratrol. Background Art

[0002] Currently, most whitening and freckle-removing products on the market rely on chemical synthetic ingredients. Although these ingredients have certain whitening effects, they also have some disadvantages. For example, some chemical ingredients may cause skin irritation or allergic reactions, especially for people with sensitive skin. In addition, although some ingredients such as 377 have significant whitening effects, they are cytotoxic and the concentration must be strictly controlled when used; and vitamin C, although highly safe, has poor stability and is easily oxidized and discolored, affecting the effect of use. Based on the above problems, researchers are eager to find a stable and safe ingredient with whitening and freckle-removing effects, and plant extracts are gradually coming into people's view.

[0003] Plant extracts, as cosmetic raw materials, have many advantages. First, plant extracts are derived from natural plants, which are safer, gentler, and suitable for all skin types, especially for consumers with sensitive skin. Secondly, the active ingredients contained in plant extracts, such as polyphenols, alkaloids, flavonoids, etc., have multiple biological activities such as antioxidant, anti-inflammatory, and antibacterial, which can give products multiple functions. In addition, plant extracts have a wide range of applications and can be used in whitening, moisturizing, anti-aging and other skin care fields. The natural properties of plant extracts meet consumers' pursuit of safety and health, and are also in line with the trend of sustainable development. The diversity of plant extracts and the richness of active ingredients provide more possibilities for the research and development of cosmetics, and help develop more efficient and safer whitening and spot-removing products. Therefore, the development of cosmetics based on plant extracts is not only a reflection of market demand, but also an inevitable trend of industry development. Summary of the invention

[0004] The purpose of the present invention is to provide a whitening and freckle-removing composition containing resveratrol, which is prepared by using plant extracts as raw materials, has high safety and has excellent whitening and freckle-removing effects.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] In a first aspect, the present invention provides a whitening and freckle-removing composition, comprising the following ingredients: resveratrol, scutellaria baicalensis extract, honeysuckle flower extract, purslane extract, yarrow extract and cassia seed extract.

[0007] Preferably, the whitening and spot-lightening composition is composed of the following components in parts by weight:

[0008] 0.1-0.5 parts of resveratrol, 3-6 parts of scutellaria baicalensis extract, 1-3 parts of honeysuckle flower extract, 1-3 parts of purslane extract, 3-6 parts of yarrow extract, and 2-5 parts of cassia seed extract.

[0009] Furthermore, the whitening and spot-removing composition is composed of the following components in parts by weight:

[0010] 0.3-0.5 parts of resveratrol, 3-5 parts of scutellaria baicalensis extract, 1-2 parts of honeysuckle flower extract, 2-3 parts of purslane extract, 3-5 parts of yarrow extract, and 3-5 parts of cassia seed extract.

[0011] Most preferably, the whitening and spot-lightening composition consists of the following components in parts by weight:

[0012] 0.3 parts of resveratrol, 4 parts of scutellaria baicalensis extract, 2 parts of honeysuckle extract, 2 parts of purslane extract, 4 parts of yarrow extract, and 4 parts of cassia seed extract.

[0013] In a second aspect, the present invention provides a whitening and spot-lightening cosmetic, wherein the whitening and spot-lightening cosmetic is composed of the following components in percentage by mass:

[0014] The whitening and spot-lightening composition comprises 3-5wt%, EDTA-2Na 0.1-0.5wt%, sodium hyaluronate 1-3wt%, glycerin 3-5wt%, propylene glycol laurate 0.1-0.5wt%, and the balance is water.

[0015] Preferably, the water is deionized water.

[0016] In a third aspect, the present invention provides a method for preparing a whitening and spot-lightening cosmetic, the specific steps of which are as follows:

[0017] S1. Add EDTA-2Na, glycerol and 2 / 5 of water into an emulsifying pot, heat to 80-85°C, keep warm for 10-15 minutes, stir evenly to obtain phase A;

[0018] S2. Add propylene glycol laurate, sodium hyaluronate, whitening and spot-removing composition and 1 / 2 of water into an emulsifying pot, heat to 35-40°C, keep warm for 10-15 minutes, stir evenly to obtain phase B;

[0019] S2. Add sodium hyaluronate, whitening and spot-removing composition and 1 / 2 of water into an emulsifying pot, heat to 35-40°C, keep warm for 10-15 minutes, stir evenly, and obtain phase B;

[0020] S3. Cool phase A and phase B to 25-30°C, add phase A, phase B and 1 / 10 of water into an emulsifying pot, stir evenly, filter the material through 400 mesh to obtain whitening and spot-lightening cosmetics.

[0021] Preferably, the water is deionized water.

[0022] Compared with the prior art, the present invention has the following beneficial effects: the present invention provides a whitening and freckle-removing composition containing resveratrol, the composition with whitening and freckle-removing effect is compounded by resveratrol, scutellaria extract, honeysuckle extract, purslane extract, yarrow extract, and cassia seed extract, and studies have found that there is a synergistic effect between resveratrol, scutellaria extract, honeysuckle extract, purslane extract, yarrow extract, and cassia seed extract; adding yarrow extract and cassia seed extract to the composition of resveratrol, scutellaria extract, honeysuckle extract, and purslane extract improves its antioxidant and tyrosinase activity inhibition effects. DETAILED DESCRIPTION

[0023] In order to better understand the present invention, the present invention is further described below in conjunction with specific application examples, wherein the terms used in the application examples are for describing specific implementation schemes and do not constitute limitations on the protection scope of the present invention.

[0024] The experimental methods in the following examples without specifying specific conditions are usually carried out under conventional conditions or conditions recommended by the manufacturer. Unless otherwise specified, the calculation is based on mass percentage and mass fraction.

[0025] Some raw materials and their sources are as follows:

[0026] Resveratrol: purchased from Merck KGaA, Darmstadt, Germany, CAS: 501-36-0;

[0027] Scutellaria baicalensis extract: purchased from Hunan Langlin Biological Resources Co., Ltd., catalog number: NAT-019;

[0028] Honeysuckle flower extract: purchased from Gansu Yishengxiang Biotechnology Co., Ltd.;

[0029] Portulaca oleracea extract: purchased from Hunan Langlin Biological Resources Co., Ltd., product number: NAT-241;

[0030] Yarrow extract: purchased from Shaanxi New Horizon Biotechnology Co., Ltd.;

[0031] Cassia seed extract: purchased from Shanyang Lianfeng Biotechnology Co., Ltd.;

[0032] Cassia obtusifolia seed extract: purchased from Shanyang Lianfeng Biotechnology Co., Ltd.;

[0033] Cassiae obtusifolia leaf extract: purchased from Xi'an Ruierli Bioengineering Co., Ltd.

[0034] The mass proportion of raw materials of the whitening and spot-removing composition is as follows:

[0035] Table 1 Whitening and spot-removing composition mass ratio

[0036]

[0037]

[0038] Note: The missing components of composition ①-⑦ are replaced by deionized water or the like. The whitening and spot-lightening cosmetics described in this application are specifically a whitening and spot-lightening essence. The mass percentage of the raw materials of the whitening and spot-lightening essence is as follows:

[0039]

[0040]

[0041] The preparation methods of the above application examples 1-5, application examples ①-⑦ and blank application examples are as follows:

[0042] S1. Add EDTA-2Na, glycerol and 2 / 5 of water into an emulsifying pot, heat to 80-85°C, keep warm for 10-15 minutes, stir evenly to obtain phase A;

[0043] S2. Add propylene glycol laurate, sodium hyaluronate, whitening and spot-removing composition and 1 / 2 of water into an emulsifying pot, heat to 35-40°C, keep warm for 10-15 minutes, stir evenly to obtain phase B;

[0044] S3. Cool phase A and phase B to 30°C, add phase A, phase B and 1 / 10 of water into an emulsifying pot, stir evenly, filter the material through 400 mesh to obtain a whitening and spot-removing essence.

[0045] Application Example ⑧ has the same formulation as Application Example 1 except for the preparation steps, which are as follows:

[0046] S1. Add EDTA-2Na, glycerin, propylene glycol laurate, sodium hyaluronate, whitening and spot-removing composition and water into an emulsifying pot, heat to 85°C, keep warm for 15 minutes, stir evenly, then cool to 30°C, filter out the material through 400 mesh, and obtain whitening and spot-removing essence.

[0047] Test 1 Tyrosinase inhibition test

[0048] The tyrosinase inhibition test was used to verify the tyrosinase inhibition ability of Application Examples 1-5, Application Examples ①-⑧ and Blank Application Example.

[0049] Tyrosinase is a key enzyme in the synthesis of melanin. Inhibiting it can effectively reduce the synthesis of melanin and play a whitening role.

[0050] The test method refers to T / GDCA 006-2021 "Guangdong Cosmetics Association Group Standard-Test Method for Tyrosinase Activity Inhibition of Cosmetic Raw Materials (In Vitro Method)".

[0051] Test samples: Application Examples 1-5, Application Examples ①-⑧ and Blank Application Examples, concentration: 10 g / L;

[0052] Kojic acid concentration: 0.005g / L.

[0053] Table 3 Tyrosinase inhibition rate

[0054]

[0055]

[0056] According to the results of Application Examples 1-5 in Table 3, the whitening and spot-removing composition provided in the embodiments of the present invention has a good effect in inhibiting tyrosinase, especially the effect of Application Example 1 is better, which shows that the whitening and spot-removing composition and whitening and spot-removing cosmetics of the present invention have a good effect in inhibiting tyrosinase.

[0057] Comparing the results of Application Example 1 with Application Examples ①-⑦, it can be seen that the use of the whitening and spot-removing composition with a specific combination and specific ratio of the present invention can significantly enhance the tyrosinase inhibition effect of the whitening composition, and the raw materials in the whitening and spot-removing composition of the present invention have a synergistic effect.

[0058] Comparing the results of Application Example 1 with those of Application Example ⑧, it can be seen that the whitening and spot-lightening cosmetics prepared using the preparation method provided by the present invention have better efficacy.

[0059] Test 2 Antioxidant Test

[0060] Fe 2+ The peroxidation model of very low density lipoprotein (VLDL) and low density lipoprotein (DL) superunsaturated fatty acids (PUFA) at the C-2 position of egg yolk phospholipids was induced, and the anti-lipid peroxidation effect of the samples was determined by the thiobarbituric acid method (TBA).

[0061] Yolk suspension: remove the white of fresh eggs, and prepare the egg yolk into a 1:1 suspension with an equal volume of pH 7.45, 0.1 mol / L sodium phosphate buffer (PBS). Stir magnetically for 10 min, and then dilute with PBS buffer to a suspension with a volume ratio of 1:25 (refrigerate for later use).

[0062] Determination method: Add 0.4mL of egg yolk suspension, 0.1mL of sample solution (concentration 10g / L), 0.4mL of FeSO4 (concentration 25mmol / L), and 3.1mL of PBS (concentration 0.1mo / L; pH 7.45) into a stoppered test tube, mix well and oscillate at 37℃ for 15min, take out and add 1mL of trichloroacetic acid aqueous solution (mass fraction 20%), centrifuge at 3500r / min for 10min, aspirate 2mL of supernatant, add 1mL of TBA (mass fraction 0.8%), stopper and place in a boiling water bath for 15min. After cooling, adjust to zero with a blank tube (replaced with 3mL of distilled water), and determine the absorbance colorimetrically at a wavelength of 532nm. The absorbance of the tube without sample (replace the sample with 0.1mL of Vc with a concentration of 1mg / mL) is A0, and the absorbance of the sample tube is A.

[0063]

[0064] Note: AOA is the peroxidation inhibition rate of the sample solution in the yolk lipoprotein lipid peroxidation system; A is the average absorbance after adding the sample to the reaction; A0 is the average absorbance of the blank group.

[0065] Table 4 Peroxidation inhibition rate

[0066] Experimental Group Inhibition rate Application Example 1 83％ Application Example 2 81％ Application Example 3 83％ Application Example 4 80％ Application Example 5 79％ Application example ① 58％ Application Example ② 32％ Application Example ③ 62％ Application Example ④ 59％ Application Example ⑤ 72％ Application Example ⑥ 74％ Application Example ⑦ 28％ Application Example⑧ 74％ VC 100％ blank 0

[0067] According to the results of Application Examples 1-5 in Table 4, the composition provided by the present invention has good antioxidant properties, especially Application Example 1 has a better effect.

[0068] Comparing the results of Application Example 1 with those of Application Examples ①-⑦, it can be seen that the combination of different components can produce a synergistic effect on the antioxidant effect, and the antioxidant effect achieved by the present invention cannot be achieved if any one of the components is omitted.

[0069] Comparing the results of Application Example 1 with those of Application Example ⑧, it can be seen that the whitening and spot-lightening cosmetics prepared using the preparation method provided by the present invention have better efficacy.

[0070] Test 3: Patch test:

[0071] The present invention experiments verify the biosafety of Application Examples 1-5, Application Examples ①-⑧ and blank application examples.

[0072] The 2015 "Technical Specifications for Safety of Cosmetics" was used as a reference standard for the irritation evaluation of cosmetics. The test method was a skin patch test, and 140 randomly distributed people aged 16-65 were tested.

[0073] Test method: Put the test substance into the patch tester, the dosage is 0.020-0.025g, cover the patch tester with the test substance on the back or forearm of the subject with non-irritating cloth-based tape, and gently press it with the palm to make it evenly adhere to the skin surface for 24 hours. After removing the test patch tester, observe the skin reaction after the indentation disappears 30 minutes later. If the result is negative, observe again 24 hours and 48 hours after the patch test.

[0074] Evaluation criteria:

[0075] Grade 0: negative reaction;

[0076] Grade 1: Suspicious reaction, only slight erythema;

[0077] Grade 2: weak positive reaction, erythema, infiltration, edema, and papules may be present;

[0078] Grade 3: Strong positive reaction, erythema, infiltration, edema, papules, and the reaction may extend beyond the test area;

[0079] Grade 4: Very strong positive reaction, obvious erythema, severe infiltration, edema, confluent herpes, and the reaction exceeds the test area.

[0080] Test results: Skin reactions were negative in all subjects.

[0081] Test 4 Human test on sensitive skin

[0082] Test substances: Application Examples 1-5, Application Examples ①-⑧ and Blank Application Examples.

[0083] Subject selection: healthy volunteers aged 21-44 years old, who meet the requirements of lactic acid sting test, voluntarily participate and sign the informed consent, and can complete the test as required during the test period are selected. Each group has 30 subjects.

[0084] Experimental method: Volunteers randomly divided both sides of their faces into test side and control side. After cleansing, the product was evenly applied to the facial skin of the test side according to the product usage instructions, and no product was used on the control side. After 28 days of trial, the volunteers reported local skin reactions. If there were any adverse reactions, dermatologists would evaluate the adverse reactions.

[0085] Result analysis: The grading of adverse skin reactions was determined according to the grading of adverse skin reactions in human trial tests specified in the "Technical Specifications for Safety of Cosmetics" (2015 edition) issued by the State Food and Drug Administration. The results are shown in Table 5.

[0086] Table 5 Human trial test results

[0087]

[0088] Combining the results of the human skin patch test in Experiment 3 with the sensitive skin test in this experiment, during the test period, no subjects in Application Examples 1-5, Application Examples ①-⑧ and Blank Application Examples experienced adverse skin reactions, indicating that Application Examples 1-5, Application Examples ①-⑧ and Blank Application Examples have good safety, are mild and non-irritating, and are suitable for people with sensitive skin.

[0089] The embodiments described above are part of the embodiments of the present application, rather than all of the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present application.

Claims

1. A whitening and spot-lightening composition, characterized in that: The whitening and spot-lightening composition is composed of the following components in parts by weight: Resveratrol 0.1-0.5 parts; 3-6 parts of Scutellaria baicalensis extract; 1-3 parts of honeysuckle flower extract; 1-3 parts of purslane extract; 3-6 parts of Yarrow extract; 2-5 parts of Cassia seed extract.

2. The whitening and spot-lightening composition according to claim 1, characterized in that: The whitening and spot-lightening composition is composed of the following components in parts by weight: Resveratrol 0.3-0.5 parts; 3-5 parts of Scutellaria baicalensis extract; 1-2 parts of honeysuckle extract; 2-3 parts of purslane extract; 3-5 parts of Yarrow extract; 3-5 parts of Cassia seed extract.

3. The whitening and spot-lightening composition according to claim 2, characterized in that: The whitening and spot-lightening composition is composed of the following components in parts by weight: Resveratrol 0.3 parts; 4 parts of Scutellaria baicalensis extract; 2 parts of honeysuckle flower extract; 2 parts of purslane extract; 4 parts of Yarrow extract; 4 parts of Cassia seed extract.

4. A whitening and spot-lightening cosmetic, characterized in that: The whitening and spot-lightening cosmetic comprises the whitening and spot-lightening composition according to any one of claims 1 to 3.

5. The whitening and spot-lightening cosmetic according to claim 4, characterized in that: The whitening and spot-lightening cosmetics are composed of the following components in percentage by mass: 3-5wt% of whitening and spot-lightening composition; EDTA-2Na 0.1-0.5wt%; Sodium hyaluronate 1-3wt%; Glycerol 3-5wt%; Propylene glycol laurate 0.1-0.5wt%; Water balance.

6. A method for preparing the whitening and spot-lightening cosmetic according to claim 5, characterized in that: The preparation method of the whitening and spot-lightening cosmetic is as follows: S1. Add EDTA-2Na, glycerol and 2 / 5 of water into an emulsifying pot, heat to 80-85°C, keep warm for 10-15 minutes, stir evenly to obtain phase A; S2. Add propylene glycol laurate, sodium hyaluronate, whitening and spot-removing composition and 1 / 2 of water into an emulsifying pot, heat to 35-40°C, keep warm for 10-15 minutes, stir evenly to obtain phase B; S3. Cool phase A and phase B to 25-30°C, add phase A, phase B, and 1 / 10 of water into an emulsifying pot, stir evenly, filter the material through 400 mesh to obtain whitening and spot-lightening cosmetics.

7. The whitening and spot-lightening cosmetic according to claim 5, characterized in that: The whitening and spot-lightening cosmetic dosage form is any one of an essence cream, an essence lotion, an essence milk, and an essence cream.

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