Eye care solution for relieving asthenopia and preparation method and application thereof

Abstract

The invention relates to the technical field of eye care liquid, in particular to eye care liquid for relieving asthenopia and a preparation method and application thereof, and the eye care liquid is prepared from peptide selenium polysaccharide, hyaluronic acid, vitamin B2, oat extract and water. The effective component of the eye mask is peptide selenium polysaccharide, so that the eye mask has a good anti-oxidation effect and can effectively remove free radicals in eyes, protect cell membranes of the eyes and maintain normal physiological functions of optic nerves. Peptide selenium polysaccharide can protect the retina and eye tissues from being damaged by the external environment and blue light, and the eye aging process is delayed. Meanwhile, metabolism of the eyes is promoted, and the normal function and health state of the eyes can be maintained. Eye protection components such as hyaluronic acid, vitamin B2 and oat essence are also added, so that eye functions can be adjusted, and asthenopia can be relieved. The traditional Chinese medicine composition can effectively improve asthenopia, and is suitable for relieving or relieving discomfort such as asthenopia, eye dryness, eye pain and the like caused by overuse of eyes such as work, study, humidification, staying up late and the like.

Description

Technical Field

[0001] The invention relates to the technical field of eye care solutions, in particular to an eye care solution for relieving visual fatigue, and a preparation method and application thereof. Background Art

[0002] With the changes in modern lifestyles, the widespread use of electronic products has led to a significant increase in people's eye-use time, and the problem of visual fatigue has become increasingly serious. Symptoms of visual fatigue include dry eyes, fatigue, blurred vision, and eye discomfort, which not only affects people's work efficiency, but may also have long-term effects on eye health. Although there are many types of existing eye care products, they are limited in their effectiveness in relieving visual fatigue, and some products may have irritation or safety issues. Therefore, the development of a safe and effective eye care solution to relieve visual fatigue has become a technical problem that needs to be solved urgently. Summary of the invention

[0003] The technical problem to be solved by the present invention is: in view of the deficiencies of the prior art, an eye care solution for relieving visual fatigue is provided, which promotes eye nutrition by adding raw materials such as peptide selenium polysaccharides and hyaluronic acid, while moisturizing the eyes, effectively relieving visual fatigue and avoiding the occurrence of dry eyes.

[0004] In order to achieve the above technical purpose, the technical solution provided by the present invention is as follows:

[0005] An eye care solution for relieving visual fatigue, the eye care solution consists of peptide selenium polysaccharide, hyaluronic acid, vitamin B2, oat extract and water.

[0006] As an improvement, the eye care solution is composed of the following components in parts by mass: 1 to 10 parts of peptide selenium polysaccharide, 0.01 to 10 parts of hyaluronic acid, 0.0001 to 0.01 parts of vitamin B2, 0.1 to 5 parts of oat extract and 1000 parts of water.

[0007] As an improvement, the peptide selenium polysaccharide is a complex of bioactive peptides and selenium.

[0008] As an improvement, the molecular weight of the hyaluronic acid is 1200-1500 kDa.

[0009] As an improvement, the oat extract is obtained by a water extraction method.

[0010] As an improvement, the vitamin B2 is of pharmaceutical grade.

[0011] The preparation method of the preparation method of the present invention comprises the following steps:

[0012] (1) dissolving hyaluronic acid and peptide selenium polysaccharide in water and stirring to obtain solution A;

[0013] (2) adding oat extract and vitamin B2 to the solution A described in step (1) to obtain solution B;

[0014] (3) Solution B is filled under sterile conditions to obtain an eye care solution.

[0015] The preparation method of peptide selenium polysaccharide in the present invention comprises the following steps:

[0016] S1. Selection and preparation of raw materials

[0017] Select a selenium source and a peptide source; the selenium source includes selenium yeast or selenium salt, and the selenium source is crushed into fine powder to increase the surface area and improve the extraction efficiency; the peptide source includes whey protein, soy protein or microbial protein;

[0018] S2. Extraction and purification of selenium

[0019] The selenium source is soaked in a dilute acid solution at room temperature to extract selenium, the extract is heated to 40°C and maintained for 1 hour, then cooled to room temperature, the solid residue is separated by centrifugation, the selenium is further purified by an ion exchange resin, the pH is adjusted to 6.0, and the selenium is adsorbed and eluted by a resin column;

[0020] S3. Peptide Extraction and Purification

[0021] The peptide source is enzymatically hydrolyzed by protease at pH 8.0 and 37°C for 4-6 hours; the enzymatic hydrolyzate is purified by ultrafiltration technology, and the permeate rich in bioactive peptides is collected; the bioactive peptides are further purified by reverse phase chromatography, the mobile phase A is 0.1% trifluoroacetic acid aqueous solution, the mobile phase B is 0.1% trifluoroacetic acid acetonitrile solution, the gradient elution conditions are 0-30 minutes 5% B, 30-60 minutes 30% B, 60-90 minutes 50% B, and the flow rate is 1 ml / min;

[0022] S4. Binding of peptides to selenium

[0023] The purified selenium solution was mixed with the bioactive peptide solution at pH 7.0 and 4°C to prevent the degradation of the peptide and the reduction of selenium; the mixed solution was gradually heated to room temperature under stirring and maintained for 24 hours to promote the binding of selenium to the peptide; a reducing agent and a chelating agent were added during the reaction to fix the binding of selenium to the peptide to form a stable peptide selenopolysaccharide;

[0024] S5: Purification of Peptide Selenose Polysaccharide

[0025] The peptide selenopolysaccharide was further purified using gel permeation chromatography (GPC) to remove unreacted peptide or selenium.

[0026] The advantages of the present invention are:

[0027] 1. The active ingredient of the present invention is peptide selenium polysaccharide, which has a good antioxidant effect, can effectively remove free radicals in the eye, protect the cell membrane of the eye and maintain the normal physiological function of the optic nerve. Peptide selenium polysaccharide helps to protect the retina and eye tissue from damage by the external environment and blue light, delays the aging process of the eye, maintains good vision, and at the same time, promotes eye metabolism, which helps to maintain the normal function and health of the eye.

[0028] 2. The present invention also adds eye protection ingredients such as hyaluronic acid, vitamin B2 and oat essence. Hyaluronic acid has the function of moisturizing and locking water, which can effectively maintain the moisture of the eyeball, promote the health of eye tissues, and relieve dryness and discomfort caused by long-term use of eyes. Vitamin B2 is an essential coenzyme in cellular energy metabolism, which helps to maintain the normal function and energy supply of eye cells, thereby supporting the normal work of the eyes. At the same time, vitamin B2 helps to promote blood circulation in the eyes, improve the oxygen and nutrient supply of eye tissues, and relieve eye fatigue caused by long-term use of eyes. Oat extract contains anti-inflammatory ingredients, such as oat alkaloids, which can help reduce eye inflammation and redness, and relieve discomfort caused by visual fatigue. Oat extract also has the characteristics of soothing the skin, which can reduce eye irritation and itching, and provide a comfortable use experience. DETAILED DESCRIPTION

[0029] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments.

[0030] The invention discloses an eye care solution for alleviating visual fatigue. The eye care solution consists of peptide selenium polysaccharide, hyaluronic acid, vitamin B2, oat extract and water.

[0031] The specific ingredients of the eye care solution are composed of the following components in parts by mass: 1 to 10 parts of peptide selenium polysaccharide, 0.01 to 10 parts of hyaluronic acid, 0.0001 to 0.01 parts of vitamin B2, 0.1 to 5 parts of oat extract and 1000 parts of water.

[0032] Among them, peptide selenium polysaccharide is a complex of bioactive peptide and selenium, and its preparation method is as follows:

[0033] S1. Selection and preparation of raw materials

[0034] A selenium source and a peptide source are selected; the selenium source includes selenium yeast or selenium salt, and the selenium source is crushed into fine powder to increase the surface area and improve the extraction efficiency; the peptide source includes whey protein, soy protein or microbial protein.

[0035] S2. Extraction and purification of selenium

[0036] The selenium source is soaked in 0.1M HCl solution at room temperature to extract selenium, the extract is heated to 40°C and maintained for 1 hour, then cooled to room temperature, the solid residue is separated by centrifugation, the selenium is further purified by ion exchange resin, the pH is adjusted to 6.0, and the selenium is adsorbed and eluted by a resin column;

[0037] S3. Peptide Extraction and Purification

[0038] The peptide source was hydrolyzed by trypsin at pH 8.0 and 37°C for 4-6 hours; the hydrolyzate was purified by ultrafiltration technology, and the permeate rich in bioactive peptides was collected; the bioactive peptides were further purified by reverse phase chromatography, the mobile phase A was 0.1% trifluoroacetic acid aqueous solution, the mobile phase B was 0.1% trifluoroacetic acid acetonitrile solution, the gradient elution conditions were 0-30 minutes 5% B, 30-60 minutes 30% B, 60-90 minutes 50% B, and the flow rate was 1 ml / min;

[0039] S4. Binding of peptides to selenium

[0040] The purified selenium solution was mixed with the bioactive peptide solution at pH 7.0 and 4°C to prevent the degradation of the peptide and the reduction of selenium; the mixed solution was gradually heated to room temperature under stirring and maintained for 24 hours to promote the binding of selenium to the peptide; a reducing agent (ascorbic acid) and a chelating agent (EDTA) were added during the reaction to fix the binding of selenium to the peptide to form a stable peptide selenopolysaccharide;

[0041] S5: Purification of Peptide Selenose Polysaccharide

[0042] The peptide selenopolysaccharide was further purified using gel permeation chromatography (GPC) to remove unreacted peptide or selenium.

[0043] Example 1

[0044] Weigh 1 g of peptide selenium polysaccharide and 1 g of hyaluronic acid, dissolve them in 1000 mL of water in turn, and stir to obtain solution A; then add 1 mg of vitamin B2 and 0.1 g of oat extract to solution A to obtain solution B, and fill solution B under sterile conditions to obtain eye care solution.

[0045] Example 2

[0046] Weigh 10 g of peptide selenium polysaccharide and 10 g of hyaluronic acid, dissolve them in 1000 mL of water in turn, and stir to obtain solution A; then add 10 mg of vitamin B2 and 5 g of oat extract to solution A to obtain solution B, and fill solution B under sterile conditions to obtain eye care solution.

[0047] Example 3

[0048] Weigh 5 g of peptide selenium polysaccharide and 6 g of hyaluronic acid, dissolve them in 1000 mL of water in turn, and stir to obtain solution A; then add 0.1 mg of vitamin B2 and 3 g of oat extract to solution A to obtain solution B, and fill solution B under sterile conditions to obtain eye care solution.

[0049] The care solution prepared in Example 2 was used to conduct an experimental test on anti-eye fatigue.

[0050] Purpose:

[0051] To evaluate the effectiveness and comfort of anti-eye fatigue eye care solution in actual use for people who use electronic products or wear contact lenses for a long time.

[0052] Experimental design:

[0053] 60 volunteers were selected to participate in the clinical trial, including people who often wear contact lenses or use electronic products such as computers and mobile phones for a long time. The volunteers were divided into two groups, of which 30 people in the experimental group used the eye protection solution prepared in Example 2, and 30 people in the control group used a certain lutein eye protection solution currently available on the market. The test period was set to 5 days.

[0054] Test method:

[0055] 1) Volunteers first clean the container cup of eye care solution.

[0056] 2) Pour 5-10ml of anti-eye fatigue eye care solution into the cup.

[0057] 3) The volunteer lies face down, places the container cup tightly around the eye, and then slowly tilts the head back to allow the care solution to come into contact with the eye.

[0058] 4) Keep the eyes moist for about 10 seconds, then lower your head to remove the container cup and pour out the used care solution.

[0059] 5) Clean the container cup and repeat the above steps for the other eye. After use, wash the container cup and air dry it for later use.

[0060] Volunteers used the eye care solution 1-2 times a day.

[0061] Observation indicators include:

[0062] 1. Improvement in visual clarity; 2. Changes in eye comfort; 3. Eye moisturizing; 4. Relief of dry eye symptoms; 5. Reduction of foreign body sensation in the eyes; 6. Reduction and alleviation of red bloodshot eyes.

[0063] Volunteers gave subjective scores on a scale of 1-5 based on their physical sensations and symptom improvement.

[0064] Scoring Rules:

[0065] 1 point - no improvement or worsening:

[0066] Volunteers did not feel any improvement after using the product, or their symptoms worsened. Visual clarity did not change or became worse. Eye comfort and moisturization did not improve, or felt more uncomfortable. Dry eyes did not get better, or felt drier. Redness in the eyes did not decrease, or looked more obvious.

[0067] 2 points - Slight improvement:

[0068] Volunteers felt slight improvements after using the product. Visual clarity improved slightly, but the effect was not significant. Eye comfort and moisturization improved, but the improvement was limited. Dry eyes were relieved, but the effect was not significant. Eye redness was reduced, but the reduction was not significant.

[0069] 3 points - Moderate improvement:

[0070] Volunteers felt moderate improvement after using the product. Visual clarity was significantly improved, but not optimal. Eye comfort and moisturizing were significantly improved, and they felt relatively satisfied. Dry eyes were significantly relieved, but dryness still occurred occasionally. Redness in the eyes was moderately reduced, and the improvement effect was obvious.

[0071] 4 points - Significant improvement:

[0072] Volunteers felt significant improvement after using the product. Visual clarity was significantly improved, close to or reaching the optimal state. Eye comfort and moisturizing were very high, and they felt very satisfied. Dry eye symptoms were significantly relieved, and they almost no longer felt dry. Red bloodshot eyes were significantly reduced, and the improvement effect was very obvious.

[0073] 5 points - Completely improved:

[0074] The volunteers felt completely improved after using the product, and all symptoms were resolved. Visual clarity was completely restored without any discomfort. Eye comfort and moisturizing were optimal, and they felt extremely satisfied. Dry eye symptoms were completely relieved without any dry feeling. Redness in the eyes completely disappeared, and the eyes looked very healthy.

[0075] Test results:

[0076] Observation Project Average score of experimental group The average score of the control group Improved visual clarity 3.7 3.3 Changes in eye comfort 4.5 3.8 Eye moisturizing 4.4 3.7 Degree of relief of dry eyes symptoms 4.0 3.2 Relief of foreign body sensation in the eye 4.2 3.1 Reduce and alleviate red bloodshot eyes 4.1 3.9 Total average rating 4.15 3.5

[0077] From the above results, it can be seen that the use sensation of the eye drops of the present invention in the control test is significantly higher than that of the control group. The visual clarity of the volunteers is significantly improved, the eye comfort and moisturizing degree are improved, the symptoms of dry eyes will be relieved, the red bloodshot eyes will be reduced, and the foreign body sensation will be alleviated. This shows that the present invention can effectively improve visual fatigue, and is suitable for relieving or alleviating visual fatigue, dry eyes, pain and other discomforts caused by excessive eye use such as work, study, humidification, staying up late, etc.

[0078] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. An eye care solution for relieving visual fatigue, characterized in that: The eye care solution consists of peptide selenium polysaccharide, hyaluronic acid, vitamin B2, oat extract and water.

2. The eye care solution for relieving visual fatigue according to claim 1, characterized in that: The eye care solution is composed of the following components in parts by mass: 1 to 10 parts of peptide selenium polysaccharide, 0.01 to 10 parts of hyaluronic acid, 0.0001 to 0.01 parts of vitamin B2, 0.1 to 5 parts of oat extract and 1000 parts of water.

3. The eye care solution for relieving visual fatigue according to claim 2, characterized in that: The peptide selenium polysaccharide is a complex of bioactive peptides and selenium.

4. The eye care solution for relieving visual fatigue according to claim 2, characterized in that: The molecular weight of the hyaluronic acid is 1200-1500 kDa.

5. The eye care solution for relieving visual fatigue according to claim 2, characterized in that: The oat extract is obtained by a water extraction method.

6. The eye care solution for relieving visual fatigue according to claim 2, characterized in that: The vitamin B2 is of pharmaceutical grade.

7. A method for preparing the eye care solution for relieving visual fatigue according to claim 1, characterized in that: The following steps are involved: (1) dissolving hyaluronic acid and peptide selenium polysaccharide in water and stirring to obtain solution A; (2) adding oat extract and vitamin B2 to the solution A described in step (1) to obtain solution B; (3) Solution B is filled under sterile conditions to obtain an eye care solution.

8. Use of the eye care solution according to any one of claims 1 to 7 in daily eye care.

9. A method for preparing peptide selenium polysaccharide according to claim 3, characterized in that: The steps include: S1. Selection and preparation of raw materials Selecting a selenium source and a peptide source; the selenium source includes selenium yeast or selenium salt, and crushing the selenium source into fine powder to increase the surface area and improve the extraction efficiency; The peptide source includes: whey protein, soy protein or microbial protein; S2. Extraction and purification of selenium The selenium source is soaked in a dilute acid solution at room temperature to extract selenium, the extract is heated to 40°C and maintained for 1 hour, then cooled to room temperature, the solid residue is separated by centrifugation, the selenium is further purified by an ion exchange resin, the pH is adjusted to 6.0, and the selenium is adsorbed and eluted by a resin column; S3. Peptide Extraction and Purification The peptide source is enzymatically hydrolyzed by protease at pH 8.0 and 37°C for 4-6 hours; the enzymatic hydrolyzate is purified by ultrafiltration technology, and the permeate rich in bioactive peptides is collected; the bioactive peptides are further purified by reverse phase chromatography, the mobile phase A is 0.1% trifluoroacetic acid aqueous solution, the mobile phase B is 0.1% trifluoroacetic acid acetonitrile solution, the gradient elution conditions are 0-30 minutes 5% B, 30-60 minutes 30% B, 60-90 minutes 50% B, and the flow rate is 1 ml / min; S4. Binding of peptides to selenium The purified selenium solution was mixed with the bioactive peptide solution at pH 7.0 and 4°C to prevent peptide degradation and selenium reduction; Under stirring conditions, the mixed solution is gradually heated to room temperature and maintained for 24 hours to promote the binding of selenium to the peptide; during the reaction, a reducing agent and a chelating agent are added to fix the binding of selenium to the peptide to form a stable peptide selenopolysaccharide; S5: Purification of Peptide Selenoselenate Polysaccharide The peptide selenopolysaccharide was further purified using gel permeation chromatography (GPC) to remove unreacted peptide or selenium.