Eye-protecting composition of lutein ester and / or zeaxanthin as well as preparation method and application of eye-protecting composition

Abstract

The invention discloses a lutein ester and / or zeaxanthin eye protection composition and a preparation method and application, and particularly relates to the technical field of nutritional agent preparation, the lutein ester and / or zeaxanthin eye protection composition comprises lutein ester and / or zeaxanthin, vitamin C, blueberry powder, D-mannitol, astaxanthin, vitamin E, selenium, glutathione and lactoferrin; according to the invention, high-purity lutein ester and / or zeaxanthin are / is used as main active components, and a plurality of auxiliary components are combined; the plant eye protection composition can significantly improve the eye protection effect and effectively prevent and improve the eye health problems such as lacrimation, crystalline lenses and maculopathy, the lutein ester and / or the zeaxanthin are / is dispersed to the nanoscale through the combination of the nano-dispersion technology and the microencapsulation technology, microcapsules are formed through wall material wrapping, and the eye protection effect of the plant eye protection composition is improved. The stability and solubility of the composition are improved, and the residence time of the active ingredients in the body is prolonged, so that the durability of the eye protection effect is enhanced.

Description

Technical Field

[0001] The present invention relates to the technical field of nutrient preparation, and in particular to an eye protection composition of lutein ester and / or zeaxanthin, and a preparation method and application thereof. Background Art

[0002] With the changes in modern lifestyles, pets, like humans, are increasingly exposed to various environmental factors in their daily activities, especially the effects of ultraviolet rays from the sun during outdoor activities, which poses new challenges to their eye health. In addition, stress, genetic factors and nutritional intake considerations all work together to affect pets' eye health, increasing the risk of tearing, lens changes and age-related eye diseases. Traditionally, some basic nutrients such as vitamins A, C and E may be added to pet food to support eye health, but these ingredients have limitations in improving eye protection effectiveness.

[0003] Lutein esters and zeaxanthin, two ingredients naturally present in the macular area of ​​the eye, are also vital for pets. They not only have strong antioxidant capacity and can effectively resist the damage of free radicals in the eye, but also help filter harmful blue light and reduce light damage to the eyes, thereby maintaining the eye health of pets. Unfortunately, the purity of lutein esters and zeaxanthin in the current eye protection products for pets on the market is often insufficient, which directly limits their protective effect on pets' eyes.

[0004] In view of this, we propose a lutein ester and / or zeaxanthin eye protection composition specially designed for pets and a preparation method thereof, aiming to more effectively promote the eye health of pets by improving the purity and bioavailability of these key ingredients. Summary of the invention

[0005] The purpose of the present invention is to provide an eye protection composition of lutein ester and / or zeaxanthin, a preparation method and application thereof, so as to solve the above-mentioned deficiencies in the technology.

[0006] In order to achieve the above-mentioned object, the present invention provides the following technical scheme: an eye protection composition of lutein ester and / or zeaxanthin, comprising lutein ester and / or zeaxanthin, vitamin C, blueberry powder, D-mannitol, astaxanthin, vitamin E, selenium, glutathione and lactoferrin.

[0007] Preferably, the purity of the lutein ester and / or zeaxanthin is not less than 98%.

[0008] Preferably, the content of vitamin C is 5%-15%, the content of blueberry powder is 5%-20%, the content of D-mannitol is 10%-30%, the content of astaxanthin is 5%-15%, the content of vitamin E is 1%-5%, the content of selenium is 0.01%-0.1%, the content of glutathione is 0.1%-1%, and the content of lactoferrin is 0.5%-2%.

[0009] Preferably, the average particle size of the lutein ester and / or zeaxanthin is less than 100 nm.

[0010] The application of eye protection composition in protecting eye health includes oral liquid, capsule and powder.

[0011] The preparation method of the eye protection composition comprises the following steps:

[0012] S1: extracting lutein esters and / or zeaxanthin from natural plants, preliminarily purifying them by a first preset method, and then increasing their purity to no less than 98% by a second preset method;

[0013] S2: Mixing the purified lutein ester and / or zeaxanthin with an appropriate amount of surfactant to form a uniform solution, dispersing the lutein ester and / or zeaxanthin solution to the nanoscale using a preset device, controlling the average particle size to be less than 100 nanometers, and preparing other auxiliary ingredients at the same time;

[0014] S3: mixing the nano-sized lutein ester and / or zeaxanthin solution with other auxiliary ingredients, vitamin C, blueberry powder, D-mannitol, astaxanthin, vitamin E, selenium, glutathione and lactoferrin in a predetermined proportion, selecting a wall material and mixing it with the mixed solution, and wrapping it in the wall material by a preset drying method to form a microcapsule or powder;

[0015] S4: During the preparation of microcapsules, controlled-release materials are added to enable the microcapsules to slowly release active ingredients under preset time or conditions, and surface modification or the addition of targeting molecules enables the microcapsules to accurately reach the ocular tissues;

[0016] S5: Customize the oral solution based on the dosage regimen and dosage form, and use honey as a carrier.

[0017] Preferably, in S1, the first preset method includes solvent extraction, column chromatography or crystallization, and the second preset method includes high performance liquid chromatography HPLC or supercritical fluid extraction SFE.

[0018] Preferably, the auxiliary ingredients include vitamin C, blueberry powder, D-mannitol, astaxanthin, vitamin E, selenium, glutathione and lactoferrin, the vitamin E is in oily or powdery form, and the selenium exists in the form of inorganic salt or organic compound.

[0019] Preferably, the preset equipment includes a high-pressure homogenizer or an ultrasonic disperser.

[0020] Preferably, the preset drying method includes spray drying, freeze drying or coacervation.

[0021] In the above technical solution, the technical effects and advantages provided by the present invention are:

[0022] By using high-purity lutein esters and / or zeaxanthin as the main active ingredients and combining them with a variety of auxiliary ingredients, the eye protection composition proposed in the present invention can significantly improve the eye protection effect, effectively prevent and improve eye health problems such as tearing, lens, and macular degeneration, and through the combination of nano-dispersion and microencapsulation technology, lutein esters and / or zeaxanthin are dispersed to the nanoscale and wrapped with wall materials to form microcapsules, which not only improves their stability and solubility, but also prolongs the retention time of the active ingredients in the body, thereby enhancing the durability of the eye protection effect. The introduction of controlled release materials enables the microcapsules to slowly release the active ingredients at a specific time or under specific conditions, and through surface modification or the addition of targeting molecules, it is ensured that the active ingredients can accurately reach the eye tissues, thereby achieving precise eye protection. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present invention. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0024] Figure 1 It is a flow chart of the present invention. DETAILED DESCRIPTION

[0025] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.

[0026] The present invention provides the following technical solutions: Figure 1 As shown:

[0027] Embodiment 1:

[0028] Raw materials preparation:

[0029] Lutein esters were extracted from marigold, initially purified by solvent extraction, and then the purity was increased to 98.5% by high performance liquid chromatography (HPLC).

[0030] Look for 99% pure vitamin C powder, blueberry powder (containing natural anthocyanins), D-mannitol, astaxanthin, oily vitamin E, inorganic selenium compounds, glutathione and lactoferrin.

[0031] Nano-processing:

[0032] The purified lutein ester is mixed with an appropriate amount of a surfactant (such as Tween 80) to form a uniform solution.

[0033] Use a high-pressure homogenizer to disperse the solution to the nanoscale and control the average particle size to be around 80 nanometers.

[0034] Microcapsule preparation:

[0035] Auxiliary ingredients are mixed in proportion (for example: vitamin C 10%, blueberry powder 15%, D-mannitol 20%, astaxanthin 5%, vitamin E 3%, selenium 0.05%, glutathione 0.5%, lactoferrin 1%).

[0036] Gelatin is selected as the wall material, mixed with the mixed solution, and then wrapped in the wall material by spray drying to form microcapsules.

[0037] Controlled release and targeted modification:

[0038] Ethyl cellulose is added as a controlled-release material during the preparation of microcapsules, so that the microcapsules slowly release the active ingredients at a specific time (such as 2 hours after a meal).

[0039] Targeting molecules (such as ocular tissue-specific antibodies) are added through surface modification to ensure that the microcapsules can accurately reach the ocular tissue.

[0040] Embodiment 2:

[0041] Raw material preparation and nano-processing (same as Example 1, but attention should be paid to the solubility of lutein esters and / or zeaxanthin in the oral solution).

[0042] Solvent selection and ratio:

[0043] Honey was selected as the solvent, and auxiliary ingredients (such as vitamin C, blueberry extract, D-mannitol, vitamin E, selenium compounds, glutathione and water-soluble forms of lactoferrin) were added in proportion.

[0044] Dispersion of Nano-sized Lutein Esters:

[0045] Nano-sized lutein esters were dispersed in the solvent using an ultrasonic disperser to ensure a uniform and stable solution.

[0046] pH adjustment and sterilization:

[0047] The pH value of the solution is adjusted to an appropriate range (eg, 6.5-7.5) and sterilized.

[0048] Packaging and quality inspection:

[0049] The oral liquid is filled into sterile containers and quality tested to ensure that the product is sterile, non-irritating and stable.

[0050] Embodiment 3:

[0051] Raw material preparation and purification:

[0052] Lutein esters are extracted from natural plants (such as marigold), or zeaxanthin is extracted from corn, or both are extracted at the same time. Solvent extraction is used as a preliminary purification method to remove most impurities. Subsequently, high performance liquid chromatography (HPLC) technology is used for further purification to ensure that the purity of lutein esters and / or zeaxanthin is not less than 98%.

[0053] Nano-processing:

[0054] The purified lutein ester and / or zeaxanthin are dissolved in an appropriate amount of solvent, and a surfactant is added to improve its dispersibility. The solution is nano-processed using a high-pressure homogenizer or an ultrasonic disperser to reduce the average particle size of the lutein ester and / or zeaxanthin to less than 100 nanometers to ensure good bioavailability and stability.

[0055] Auxiliary ingredients preparation:

[0056] Prepare other auxiliary ingredients, including vitamin C (powdered, content adjusted according to the formula), blueberry powder (content adjusted according to the formula), D-mannitol (as a sweetener and filler), astaxanthin (powdered, content adjusted according to the formula), vitamin E (oil or powdered, selected according to solubility), selenium (in the form of sodium selenate or selenium yeast), glutathione (powdered), and lactoferrin (powdered). Make sure all ingredients meet food safety standards.

[0057] Mixing and Drying:

[0058] The nano-sized lutein ester and / or zeaxanthin solution is mixed evenly with the above-mentioned auxiliary ingredients in a predetermined ratio. An appropriate wall material (such as maltodextrin, sodium alginate, etc.) is selected, and after the mixed solution is mixed with the wall material, it is dried by spray drying or freeze drying technology. During spray drying, parameters such as spray rate, drying temperature and air flow velocity need to be adjusted to obtain the ideal particle size and dispersibility. During freeze drying, the mixed solution needs to be frozen into a solid state, and the water is sublimated under vacuum conditions to obtain a dry powder.

[0059] Crushing and screening:

[0060] The dried powder may contain large agglomerates, which need to be crushed by a pulverizer. Subsequently, a vibrating screen or airflow screen is used for screening to remove particles that do not meet the particle size requirements to ensure the uniformity and consistency of the powder.

[0061] Packaging and storage:

[0062] Put the sieved powder into a sealed container to avoid direct contact with air, moisture and light. Store in a cool, dry and dark environment to ensure the stability and shelf life of the product.

[0063] Quality Control and Testing:

[0064] Strict quality control and testing are required throughout the entire preparation process, including raw material purity testing, particle size testing after nano-processing, mixing uniformity testing, moisture content testing after drying, and microbial testing and stability testing of the final product, etc., to ensure that the product meets relevant standards and regulations.

Claims

1. An eye protection composition of lutein ester and / or zeaxanthin, characterized in that: Includes lutein esters and / or zeaxanthin, vitamin C, blueberry powder, D-mannitol, astaxanthin, vitamin E, selenium, glutathione and lactoferrin.

2. The eye protection composition of lutein ester and / or zeaxanthin according to claim 1, characterized in that: The purity of the lutein ester and / or zeaxanthin is not less than 98%.

3. The eye protection composition of lutein ester and / or zeaxanthin according to claim 1, characterized in that: The content of vitamin C is 5%-15%, the content of blueberry powder is 5%-20%, the content of D-mannitol is 10%-30%, the content of astaxanthin is 5%-15%, the content of vitamin E is 1%-5%, the content of selenium is 0.01%-0.1%, the content of glutathione is 0.1%-1%, and the content of lactoferrin is 0.5%-2%.

4. The eye protection composition of lutein ester and / or zeaxanthin according to claim 1, characterized in that: The average particle size of the lutein ester and / or zeaxanthin is less than 100 nm.

5. Use of the eye protection composition according to any one of claims 1 to 4 in protecting eye health, characterized in that: Includes oral liquid and powder.

6. The method for preparing the eye protection composition according to any one of claims 1 to 4, characterized in that: The steps include: S1: extracting lutein esters and / or zeaxanthin from natural plants, preliminarily purifying them by a first preset method, and then increasing their purity to no less than 98% by a second preset method; S2: Mixing the purified lutein ester and / or zeaxanthin with an appropriate amount of surfactant to form a uniform solution, dispersing the lutein ester and / or zeaxanthin solution to the nanoscale using a preset device, controlling the average particle size to be less than 100 nanometers, and preparing other auxiliary ingredients at the same time; S3: mixing the nano-sized lutein ester and / or zeaxanthin solution with other auxiliary ingredients, vitamin C, blueberry powder, D-mannitol, astaxanthin, vitamin E, selenium, glutathione and lactoferrin in a predetermined proportion, selecting a wall material and mixing it with the mixed solution, and wrapping it in the wall material by a preset drying method to form a microcapsule or powder; S4: During the preparation of microcapsules, controlled-release materials are added to enable the microcapsules to slowly release active ingredients under preset time or conditions, and surface modification or the addition of targeting molecules enables the microcapsules to accurately reach the ocular tissues; S5: Customize the oral solution based on the dosage regimen and dosage form, and use honey as a carrier.

7. The method for preparing the eye protection composition according to claim 6, characterized in that: In S1, the first preset method includes solvent extraction, column chromatography or crystallization, and the second preset method includes high performance liquid chromatography HPLC or supercritical fluid extraction SFE.

8. The method for preparing the eye protection composition according to claim 6, characterized in that: The auxiliary ingredients include vitamin C, blueberry powder, D-mannitol, astaxanthin, vitamin E, selenium, glutathione and lactoferrin. The vitamin E is in oily or powdery form, and the selenium exists in the form of inorganic salt or organic compound.

9. The method for preparing the eye protection composition according to claim 6, characterized in that: The preset equipment includes a high-pressure homogenizer or an ultrasonic disperser.

10. The method for preparing the eye protection composition according to claim 6, characterized in that: Predetermined drying methods include spray drying, freeze drying or coacervation.

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