Manufacturing method of product with crop high-temperature-resistant function

Abstract

The invention discloses a production method of a product with a crop high-temperature-resistant function, and the method comprises the following steps: S1, screening and identifying antioxidant components in a natural plant extract, S2, producing the antioxidant components by using a microbial fermentation technology, and S3, compounding the antioxidant components with trace elements required by crop growth, s4, packaging the compound based on a thermostable carrier material to protect the biological activity of the antioxidant component, and S5, applying the compound to crops to form a layer of protective film on the surfaces of the crops to improve the antioxidant enzyme activity of the crops and reduce cell damage; the production method of the product with the crop high-temperature-resistant function solves the problem that in the prior art, under long-term high-temperature stress, the activity of antioxidant enzymes in crops can be gradually reduced. And along with the continuous high temperature, the balance of an anti-oxidation system may be destroyed, the free radical scavenging rate is reduced, and cells are damaged.

Description

Technical Field

[0001] The invention relates to the field of agricultural biotechnology, and in particular to a method for preparing a product with crop high temperature resistance function. Background Art

[0002] In the agricultural field, climate change, especially global temperature rise, has brought severe challenges to crop production. High temperature stress is one of the main reasons for poor crop growth and reduced yield. To address this problem, the prior art has developed a method of adding specific biostimulants or regulators to enhance the adaptability of crops to high temperature environments. These biostimulants or regulators can promote the synthesis of heat shock proteins in plants or enhance antioxidant capacity, thereby reducing the adverse effects of high temperatures on crops.

[0003] In this method, the use of biostimulants or regulators is mainly achieved by regulating the antioxidant system in the crop. For example, certain substances can promote the synthesis of antioxidant enzymes, thereby enhancing the ability of crops to remove free radicals in the body. These measures can effectively protect crops from oxidative stress and reduce cell damage under short-term high temperature conditions. However, the existing technology still faces some limitations in practical applications, especially under long-term high temperature stress, the activity of antioxidant enzymes in crops will gradually decrease. As the high temperature continues, the balance of the antioxidant system may be destroyed, the free radical scavenging rate decreases, resulting in cell damage, which in turn has a negative impact on the normal growth and development and yield of the crop. Therefore, although the existing technology has improved the heat resistance of crops to a certain extent, its effect is still not ideal when facing long-term high temperature conditions. How to further improve the antioxidant capacity of crops under continuous high temperatures and maintain the long-term stability of the antioxidant system is still a problem that needs to be solved in the existing technology. Summary of the invention

[0004] The purpose of the present invention is to provide a method for preparing a product with high temperature resistance function for crops, so as to solve the problems existing in the prior art.

[0005] To achieve the above object, the present invention provides the following technical solution: a method for preparing a product with crop high temperature resistance function, the method comprising: S1, based on screening and identification of antioxidant components in natural plant extracts; S2, producing the antioxidant component by using microbial fermentation technology; S3, compounding the antioxidant component with trace elements required for crop growth; S4, encapsulating the compound based on a heat-stable carrier material to protect the biological activity of the antioxidant component; S5. Applied to crops, it forms a protective film on the surface of crops, increases the antioxidant enzyme activity of crops, and reduces cell damage; Wherein, the extract in step S1 is made of the following raw materials in parts by weight: 100-150 parts of trehalose, 5-10 parts of mineral source fulvic acid, 50-100 parts of complex sugar alcohol, 300-400 parts of calcium acetate, 1-5 parts of ectoine, and 400-500 parts of distilled water; The trehalose production process is as follows: starch hydrolysate is converted into trehalose by utilizing the action of maltooligosaccharide, trehalose synthase and maltooligosaccharide-based trehalose hydrolase.

[0006] Preferably, the step S3 of compounding the antioxidant component with trace elements required for crop growth comprises: Identify the effects of specific trace element combinations on plant enzyme systems; Identify the optimal addition amounts of trace elements and their mutual ratios; Compounding is done based on the determination of the synergistic effect of trace elements on antioxidant components; The final proportion formula is determined based on the interaction mechanism between specific elements.

[0007] Preferably, the process of identifying the optimal addition amount of trace elements and their mutual ratios comprises: Select at least two candidate trace elements, and preliminarily determine the experimental interval according to their usual content range in nature, assuming that candidate element a is A and candidate element b is B; Test the effects of various elements at different concentration gradients on the stress resistance of target plants; Screen out the optimal A and B concentrations that significantly improve the target enzyme activity; Based on the maximization of the activity value of the target enzyme, the ideal relative concentration ratio between A and B was analyzed by linear regression.

[0008] Preferably, the process of analyzing the ideal relative concentration ratio between A and B by linear regression comprises: Collect the enzyme activity data corresponding to the mixture of elements A and B obtained from different experimental batches; Draw a curve fitting graph to show the relationship between A and B, and find the best fit point; Set the theoretical value Y that b should reach when a is X, where X represents a specific amount of element A and Y represents the theoretical amount of element B that needs to be added. The optimal ratio that meets the above calculation requirements is recorded and used for further tests to verify the performance of the antioxidant composition.

[0009] Preferably, the antioxidant composition performance verification process in the further test includes: Predict the effect of the new ratio scheme in plants based on the established mathematical model; Indoor simulation test to verify whether the actual effect of the prediction scheme meets the expected standards; If the test results show that the improved ratio significantly improves the target enzyme activity level, it will enter the field trial stage; In the actual crop environment, the effectiveness of the final optimized version is tested through comparative experiments, and adjustments are made to make it more adaptable to natural conditions.

[0010] Preferably, the prediction of the effect of the new ratio scheme in plants based on the established mathematical model includes: Import the experimental data into the established mathematical equation, that is, when the input variable E changes, the response variable D will change by ΔD / E=Z, where E represents the change factor under external high temperature conditions; The above formula is used to simulate the ability of the new formulation to resist adverse environment; If the ΔD / E ratio is low or close to the theoretical optimum, the model is considered reliable and can be applied to future R&D designs; If the value is too large, you need to continue to modify the formula.

[0011] Preferably, the field experiment condition judgment rule is: when the crop is subjected to external extreme temperature stimulation T, the content of protective substances F inside the plant increases by G in the state with added compound liquid compared with that without compound liquid, then it is considered that the anti-temperature damage effect is enhanced, where T refers to the high temperature intensity in the external environment, F refers to the mass of specific antioxidants in the cells obtained by detection, and G represents the increase in F.

[0012] Preferably, the specific evaluation method in the field experiment includes: Establish control areas containing multiple temperature gradients; The efficacy of the compound was compared based on the plant growth indices measured under different treatment levels, and the growth rate L under normal conditions was compared with the growth rate R after treatment for comparative analysis; If under high temperature S, the R of the plant after adding the compound is at least 5 times higher than that of the normal growth L (R / L≥1.5), it is considered that this solution effectively improves temperature resistance.

[0013] It can be seen from the above technical solution that the present invention has the following beneficial effects: The method for preparing the product with crop high temperature resistance function comprises the following steps: screening and identifying antioxidant components in natural plant extracts, producing the antioxidant components by using microbial fermentation technology, compounding the antioxidant components with trace elements required for crop growth, encapsulating the compound by using a heat-stable carrier material to protect the biological activity of the antioxidant components, applying the compound to crops, forming a protective film on the surface of the crops, improving the antioxidant enzyme activity of the crops, reducing cell damage, helping the crops to maintain normal physiological functions under high temperature conditions, reducing growth inhibition and yield reduction caused by high temperature, and enhancing free radical scavenging ability, thereby alleviating oxidative stress reactions under high temperature stress, reducing cell damage, protecting crop cell structure and function, delaying the rate of decline in crop antioxidant enzyme activity, maintaining the balance of the crop antioxidant system, effectively reducing the risk of cell damage caused by high temperature stress, significantly improving the growth and development of crops, reducing fruit deformity or premature aging caused by high temperature, and ultimately improving the yield and quality of crops, solving the problem in the prior art that the antioxidant enzyme activity in crops under long-term high temperature stress gradually decreases. As high temperatures continue, the balance of the antioxidant system may be disrupted, the free radical clearance rate may decrease, and this may lead to cell damage. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 The figure is a flow chart of the method of the present invention. DETAILED DESCRIPTION

[0015] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0016] like Figure 1 As shown, a method for preparing a product with crop resistance to high temperature includes the following steps. This process is to develop a product specifically for enhancing the antioxidant enzyme system of crops in high temperature weather, and its main purpose is to reduce the oxidative stress reaction in plant cells caused by long-term exposure to high temperature environment and a series of adverse reactions associated with it.

[0017] The first step in the process is to screen and identify compounds with excellent antioxidant capacity from natural plant extracts. This means that we need to examine a series of bioactive substances extracted from plants to see if they are effective in resisting heat stress, test the effectiveness of different substances in removing excessive reactive oxygen species through experiments, and select the best ones for further analysis of structure and bioavailability, etc. For example, researchers can isolate and purify a variety of phenolic acids, flavonoids and polyphenol compounds from green tea, pomegranate peel or black ginger root.

[0018] The next step is to use modern biological technology to produce substances with high antioxidant properties in large quantities to meet the needs of large-scale agricultural applications after identifying them. This usually involves selecting specific microbial strains and designing optimal growth conditions to induce them to produce the desired target product. In specific implementation, a brewer's yeast that can efficiently produce γ-tocopherol may be selected as a producer, cultured under sterile conditions, and adding suitable inducers to promote the accumulation of the required biomacromolecules such as carotene or specific fat-soluble vitamins; beneficial bacteria such as Lactobacillus, Bacillus or blue-green algae groups may also be considered.

[0019] The next stage is to process the final product together with the nutrients required by the crops. Simply put, the natural secondary metabolites obtained above are mixed with mineral elements such as potassium, phosphorus, iron, sulfur, manganese, boron, molybdenum, zinc, etc. in a certain ratio to improve the health of the crops. For example, if the previous production produces a complex rich in β-carotene and γ-tocopherol, it may be mixed with a 0.5% ratio of magnesium sulfate and potassium dihydrogen phosphate solution and stirred until it is completely dissolved, and then supplemented with other trace elements to form a liquid preparation for filling and storage for standby spraying and application.

[0020] Next, in order to ensure that the above formula can play a long-term role in actual agricultural operations without losing its effectiveness, it is necessary to use a carrier material to package it to prevent oxidative degradation and other problems. Usually, high polymers such as polyethylene, polycaprolactone, or natural polysaccharide polymers such as sodium alginate, or even some mineral powders can be used as a protective shell to wrap it. If the above-mentioned content is in the form of a suspension preparation of beta-carrot plus gamma-tocopherol, it may be concentrated to a certain viscosity first, and then soaked overnight into a pre-cut polyethylene glycol film sheet to naturally solidify and dry to make a sheet fertilizer sticker-like finished product.

[0021] Finally, it is applied to plants. The specific implementation method is to evenly distribute and spread it on the ground surface at a specified interval or cover both sides of the leaves with a mist spray gun to form a uniform thin layer. With the evaporation of sunlight, this small molecule containing super antioxidant ingredients will slowly penetrate into the cells to participate in regulating the photoperiod cycle, inhibiting the premature aging process, reducing the degree of tissue necrosis caused by high temperature, stimulating the increase of chlorophyll content and indirectly improving the net photosynthetic efficiency, thereby enhancing the ability of crops to resist adverse external conditions. Assuming that the sheet fertilizer mentioned above has been tested to determine the safety range, farmers will generally be guided to spread three kilograms of standard packaging specifications per hectare of land and start working in the sunny afternoon until the sun sets in the evening to complete all operating procedures so that it can quickly dissolve and penetrate to exert its efficacy when the temperature drops at night; of course, more modern means can also be adopted, that is, remote-controlled drones carrying special dispersers can be used for high-altitude uniform delivery, making the product distribution more balanced and greatly saving the cost of human and material resources.

[0022] In general, the invention aims to create a new generation of high temperature resistant plant protection products that integrate natural essence factors and are improved through scientific processing. The entire production process strictly follows the principle of biocompatibility and safety while constantly pursuing formula innovation to break through existing limitations and challenge the bottleneck problems of traditional agricultural models, providing a reliable support foundation for the sustainable construction of agricultural production systems.

[0023] Next, the present invention describes compounding the antioxidant ingredients with trace elements required for crop growth. According to the method for making a product with crop resistance to high temperature, the effect of a specific trace element combination on the plant enzyme system is identified; the key to this step is to determine the key enzymes required for plants to maintain their physiological functions at high temperatures, and then study the mechanism of action between different trace elements and these enzymes and how to improve the tolerance of plants under adverse conditions. For example, in one embodiment, a series of experiments can be used to determine the importance of zinc and manganese in improving the effectiveness of the antioxidant system of a specific crop.

[0024] The second step is to identify the optimal amount of trace elements and their mutual ratios. By precisely controlling the ratios and dosages of different trace elements such as manganese, zinc and copper, the goal is to maximize the antioxidant capacity while ensuring that no negative effects or excessive amounts of them cause plant poisoning. Specifically, during the soaking process of rice seeds, researchers will use different concentration gradients for comparative analysis to derive the most suitable combined concentration ratio for enhancing its heat resistance - for example, a certain proportion of zinc ions Zn 2 in each liter of solution. 2+ The best range is 0.1mg / L to 0.3mg / L, accompanied by an appropriate proportion of iron Fe 2+ As a supplement.

[0025] Based on the first two steps, the ratio between trace elements and selected antioxidants is further investigated and optimized, and the third step is more focused on how to make this ratio work most efficiently. For example, in the design of a product containing vitamin C as a basic antioxidant, the best formulation strategy is determined by testing the differences in plant responses caused by different addition methods and timings in a laboratory environment: adding them simultaneously or adding them at different times. This requires detailed data collection and analysis before formulating a plan to ensure maximum effect.

[0026] Finally, based on the above exploration, the fourth step is to regress and optimize the final ratio based on the synergistic effect between the discovered elements to form a complete product formula guideline. Since various factors may affect each other, it is ultimately necessary to make fine adjustments based on the actual environment, such as soil conditions, weather and other factors, to achieve the most ideal crop protection effect. Assuming that it was discovered in the early stage that iron and selenium have a synergistic enhancement effect, this interaction will be comprehensively considered when determining the formula ratio to form a scientific and reasonable compound system. For example, the Se:Fe molar ratio in the final product is stipulated to be approximately 0.5:1. This ensures that the antioxidant effect is fully exerted while taking into account the plant's demand for iron nutrition. This design is based on the fact that the iron element can help increase chlorophyll content and reduce the negative impact of high temperature from existing experimental results.

[0027] Next, the process of identifying the optimal addition amount of trace elements and their mutual ratios of the present invention is described. The process mainly includes selecting candidate trace elements, determining the experimental interval, testing the effects of different concentrations on target plants, screening the optimal concentration, and finally determining the ideal relative concentration ratio.

[0028] At least two candidate trace elements are selected and the experimental interval is set according to their usual content in nature, which is to ensure the rationality and scientificity of the experiment. For example, in one embodiment, zinc (Zn) and iron (Fe) are selected as candidate trace elements, and different levels of zinc (Zn) and iron (Fe) are set according to their average concentration distribution in general soil, for example, the initial concentration of zinc is set to cover the range of 1 ppm to 10 ppm from low to high, and iron is set between 2 ppm and 20 ppm.

[0029] Subsequently, the effects of various elements under different concentration gradients on the high temperature resistance of specific crop varieties are tested. Specifically, wheat can be used as the test object. Under controlled laboratory conditions, the external temperature can be adjusted to observe and record the growth status and heat resistance of crops under different zinc and iron concentration ratios. This stage focuses on analyzing the changes in the activity of target enzymes such as catalase (CAT) and superoxide dismutase (SOD) to evaluate the ability to resist adversity, with the aim of screening out the best Zn and Fe concentration combination that significantly improves the activity of these key enzymes.

[0030] Furthermore, the optimal Zn and Fe concentrations that significantly improve the target enzyme activity are screened out. In this process, multiple tests are required to statistically analyze the overall performance of antioxidant enzymes such as CAT and SOD under different ratios. For example, after experiments, it may be found that when Zn5ppm + Fe15ppm, wheat plants show the best stress resistance and higher CAT and SOD activity values, so this group of concentrations is considered to be the best ratio with potential.

[0031] Finally, based on the principle of maximizing the activity value of the target enzyme, the ideal relative concentration ratio between Zn and Fe is calculated through mathematical models such as linear regression. This step involves establishing a correlation function model with one or several main enzymatic reaction rates as dependent variables and the respective amounts of two metal ions as independent variables. Assume that Y=αA+βB, Y represents the enzyme activity index, and the coefficients α and β represent the contribution degree or sensitivity factor to the two elements respectively; A and B correspond to the measured values ​​of zinc ion concentration and iron ion concentration respectively. Through the optimization algorithm, the numerical solution of the appropriate ratio of the two ions under the optimal conditions α / β=A / B can be obtained, so that Y is maximized, that is, the optimal condition configuration scheme for improving the effect of plant resistance to external adverse environmental influences is achieved.

[0032] Next, the process of analyzing the ideal relative concentration ratio between A and B by linear regression of the present invention is described. The process includes multiple key steps, and scientific methods are used to ensure that the product formula can effectively impart high temperature resistance to crops. The first step is to collect enzyme activity data measured during the mixing process of two elements A and B obtained from different experimental batches. These data come from multiple experiments, in which the ratio of A and B and their respective absolute concentrations are changed respectively, and the enzyme activity of the resulting reactants is measured under standard conditions.

[0033] Subsequently, based on the collected data, a curve is drawn showing the relationship between the concentration ratio of A and B and the obtained results, so as to fit the curve to find the best fit point. Specifically, the best fit point is the concentration ratio combination position with the highest enzyme activity. This helps to determine the most ideal state of interaction between A and B, in which the complementarity of the two is maximized. For example, in an experimental batch, the ratio of A to B and the corresponding measured enzyme activity level of each group under different set conditions are recorded. With the accumulation of experimental data, and through appropriate software or methods to process and analyze it, a fitting image of the relationship between A and B and its inflection point are obtained.

[0034] Next, based on the mathematical model of the above curve, a formula is set, which means that when the concentration of a is X, in order to obtain the ideal compound effect, the corresponding concentration of b should be Y. Here, parameter X is the amount or concentration value of substance A given under specific experimental conditions; and parameter Y indicates the concentration value of substance B required to achieve the optimal high temperature resistance effect under the corresponding conditions. For example, in a certain research case, if the concentration of A is fixed at a certain level in the experiment (as the X parameter), the conditions for the enzyme activity to reach the peak are found, and it is found that in this case, the amount of B needs to be adjusted to match it (as the Y value). At this time, the formula can help understand the connection between the variables in different experimental conditions and derive a new optimal solution combination.

[0035] The last step is to apply these conclusions to actual production or experiments at the experimental operation level: select the A-B ratio that obtains the highest enzyme activity level under the conditions described by the above formula for further practical verification and adjustment and improvement of the process. In this scenario, assuming that the optimal ratio of X1:A to Y1:B is obtained in the previous experiment, this result will become the basis for the next round of in-depth research and development, and will be continuously optimized through more experiments until higher requirements or standards are met. The entire process rigorously and systematically uses mathematical and chemical principles to scientifically optimize the configuration of raw materials, thereby improving the actual performance indicators of the products under study.

[0036] Next, the performance verification process of the antioxidant composition in the further test of the present invention is described. First, the effect of the new ratio scheme in plants is predicted based on the established mathematical model. This link predicts the performance of different formula ratios in actual crops by inputting data of different proportions into the mathematical model and combining existing research. The parameter is usually the ratio of various antioxidant concentrations, which can range between 0 and 1, and the ratio is optimized according to specific needs. The purpose of this formula is to simulate the plant response under different conditions to ensure the accuracy of the prediction results. For example, in one embodiment, the wheat antioxidant system is simulated, and different proportions of antioxidant ingredients such as ascorbic acid, α-tocopherol and their ratios are set to explore the most favorable pairing combination.

[0037] This is followed by the indoor simulation test phase, in which a series of control experiments are conducted to verify the actual effect of the mathematical prediction. This step is specifically reflected in the configuration of the ideal ratio predicted by the above model into an antioxidant solution and applying it to the target crop, observing target indicators such as the scavenging rate of active oxygen free radicals and changes in peroxidase content. Assuming that the prediction shows that a certain configuration can increase CAT (catalase) in wheat leaves, it is necessary to verify it under room temperature conditions. If the indoor test results do show an improvement effect, it can be further advanced to the next stage.

[0038] When indoor tests show that the optimized combination formula has been significantly improved compared to the previous one, so that the activity of the target enzyme reaches the expected or higher level, the field experiment part will begin. The field environment is more variable and closer to actual planting. At this stage, actual planting area tests are carried out, the above formula is sprayed on crops, and the plant conditions and yields in different growth cycles are recorded to comprehensively evaluate the effect. Specifically, field tests are carried out under a certain high temperature stress to test whether the optimized stress-resistant combination material can enable the experimental group of crops to obtain higher photosynthesis efficiency than the control and grow stably, thereby proving the applicability and practicality of the program.

[0039] The last stage is to adjust the details of the strategy based on the information collected during the field planting process to better adapt to various natural conditions, so that the developed products can effectively play the role of resisting high temperature damage in different time and space conditions. In short, through careful consideration and perfect adjustment of each link, we can ensure that the final product has both ideal efficacy and is easy to promote and use on a large scale. If it is found that the humidity difference in certain areas significantly affects crop absorption, it is necessary to appropriately modify the amount of additives so that the overall system can adapt to the challenges of diverse climate conditions to a greater extent.

[0040] Next, the effect of the new ratio scheme in plants predicted by the present invention based on the established mathematical model is described. The step includes first importing the relevant experimental data obtained from actual observations into a pre-constructed mathematical equation. This step expresses how changes in external conditions such as radiation and humidity act on a specific response variable D through an equation. Here, the equation form is set to ΔD / E = Z, where E is a factor representing the degree of change in the temperature and other related conditions in the environment, which can range from small fluctuations to drastic changes. Z is defined as the rate of change of the target output indicator, that is, the ratio of the change in the effect of enhancing the temperature resistance of crops; ideally, Z is expected to approach zero, which means that the heat resistance of crops is minimally affected by changes in external environmental conditions, which indicates that the formulation optimization of the heat-resistant agent is in place.

[0041] When using this formula, the effect of different ingredient ratios on improving the thermal inversion resistance will be simulated, such as simulating the dynamic changes of plant leaf transpiration rate (here taken as a specific example of D) under the condition of adding certain trace elements, so as to verify the effectiveness and feasibility of the improved formula. If the calculated ΔD / E value is relatively low or close to the best result preset in the laboratory, it is determined that this mathematical model is accurate enough and can be used to guide the subsequent product formula development iteration stage. On the contrary, when the obtained results show a large gap or do not meet expectations, it is necessary to try to adjust again until a combination ratio that makes the objective function perform well can be found. For example, in one embodiment, after setting a series of test schemes, it is found that a certain potassium-containing formula significantly slows down the increase in plant transpiration rate (ΔD is reduced by 0.01 units) compared with the control group under the same increase in radiation (E=0.3 units), and the calculation results show that Z= 0.033 is less than the preset optimal limit of 0.1, then the component design is determined to be a feasible candidate, and if Z exceeds 0.1 for other schemes, it means that it still needs to be improved to achieve better performance. In this way, the optimization experiment is iterated and optimized until satisfactory results are achieved.

[0042] Specifically, suppose a new additive is developed to improve the ability of crops to tolerate normal growth under conditions of sustained drought and strong light. The experimental design needs to monitor the differences in crop physiological responses between the treated area and the untreated area, such as measuring the rate of chlorophyll loss between the two, and then estimate the effects of new mixing ratios based on these experimental validation sets, and adjust the ingredients until the lowest impact is achieved to determine the best practice configuration. In this way, each test can be used to deduce the potential effects under new conditions based on the formula, guiding the direction of subsequent adjustments in order to achieve a more effective goal of improving heat resistance.

[0043] Next, the test condition judgment rule before the outdoor practical application of the present invention is described as follows: when the crop is subjected to an external extreme temperature stimulus T, the content of the protective substance F inside the plant increases by G when the compound liquid is added compared to when the compound liquid is not added, then it is considered that the anti-temperature damage effect is enhanced, where T refers to the high temperature intensity in the external environment, and F refers to the specific antioxidant mass in the cell obtained by detection. In order to realize this test, the following steps are required.

[0044] The first step includes selecting appropriate crops as experimental subjects and preparing corresponding test fields; the second step is to randomly divide the same test field into two areas A and B; the third step is to regularly and evenly apply the target product (i.e., a composite solution with high temperature resistance) to the plants in area A, while area B is not treated. The setting range of the extreme temperature stimulus T here is usually between 35°C and 45°C, which is adjusted according to the common peak temperature in summer in specific areas; the optimal temperature point may be 38°C or 42°C to simulate the impact of high temperature in summer on crops under real conditions. Before detecting the antioxidant content F in plants, they need to undergo a sufficient sun exposure process to induce a heat shock reaction.

[0045] For example, in one embodiment, rice seedlings that grow well and uniformly are divided into two groups for treatment: the experimental group is sprayed with a special high temperature resistant compound solution and placed in a greenhouse with a simulated temperature rise to keep the temperature stable at 40°C; the control group continues to grow under normal conditions but does not receive any special solution treatment. After a period of time, samples from the two groups are collected for analysis, and the concentration of antioxidant components such as SOD (superoxide dismutase) in the samples is tested by scientific equipment such as high performance liquid chromatography or ultraviolet visible spectrometer to obtain the data difference G before and after the test.

[0046] If the data shows that the level of specific antioxidant substances in the plants treated with the compound solution increases by G under high temperature conditions (T=40℃), it means that the proposed product does help to enhance the target organisms' resistance to damage caused by rising temperatures, verifies the effectiveness of the aforementioned rules, and lays a good data foundation for further large-scale outdoor applications. This step not only verifies whether the solution's own mechanism of action is effective, but also confirms whether its potential application value meets market demand and environmental challenges.

[0047] Next, the process of determining the final application conditions of the present invention is described as follows: examine whether the performance of the compound solution is consistent under planting conditions in multiple regions; fine-tune the component ratio according to the specific farmland conditions, such as soil acidity and alkalinity, water hardness and other influencing factors that need to be considered; the optimal combination must meet **For a planting base X under specific high temperature conditions H, the relationship between the application amount P of the compound and its effect C on improving cell survival rate should be C>K×P+J, where H is the intensity of the high temperature environment, X is a specific region, P is the application amount, C represents the rate of increase in the probability of cell survival, and K and J represent adjustment parameters; perform a series of control experiments to confirm that the optimized parameters have a wide range of applicability.

[0048] To examine whether the compound solution performs consistently under planting conditions in multiple regions, it is necessary to first compare and test the effectiveness of a specific compound solution in different geographical environments. This ensures that the effect of the compound solution can be maintained above a certain level in any environment, enhancing the applicability and reliability of the formula. For example, in field trials in the hot and humid southern regions of China and the drier northern regions, this hypothesis was verified by comparing and observing the differences in crop growth.

[0049] Fine-tuning the ratio of ingredients according to the specific conditions of farmland, such as soil acidity, water hardness and other influencing factors, means adjusting the composition ratio of the compound solution according to the specific properties of the soil under different geographical conditions, including pH value and water ion hardness and other factors. Specifically, if the soil is found to be alkaline in some areas, it can be adjusted by appropriately reducing the proportion of alkaline components or adding appropriate amounts of acidic substances such as ammonium sulfate or acid rain regulators to meet the needs of specific plots, ensuring that the active ingredients can be better absorbed, thereby achieving a more ideal effect.

[0050] The optimal combination must satisfy that for a planting base X under a specific high temperature condition H, the relationship between the amount of compound applied P and its effect C on improving cell survival should be C>K×P+J. Here, the parameter K represents the cell survival rate change constant corresponding to the unit fertilizer amount, and J represents the minimum cell survival probability increase under natural growth or environmental baseline. H is a quantitative unit of measurement used to characterize the intensity of summer heat stress, which can be divided into a continuous spectrum from the lowest to the highest. The higher the value, the more severe the temperature conditions; similarly, the regional characteristic X refers to the unique set of conditions formed by different plots due to geographical location, water source conditions, etc. This formula ensures that the additional gain brought by fertilization under given environmental conditions is significant enough. For example, when applying the model in a scenario where H=3 (representing strong heat radiation) and X is located in a certain arid zone, if an appropriate initial P value is set and a survival probability growth curve is observed that is at least 3% higher than the linear estimate (this is determined by the assumed target gain K value and the baseline correction term J), then it is considered that the best practice ratio has been found.

[0051] A series of controlled experiments are carried out to verify the general validity of the results obtained, and to clarify the degree of interaction between the operating parameters and the actual benefit-output ratio through data comparison of the control group, so as to accumulate a reliable data set for the subsequent large-scale commercial production plan deployment. For example, in a controlled laboratory environment, high temperature conditions are simulated and compared with the blank untreated population, the growth rate and root development of seeds treated with the optimized formulation product are compared and analyzed in the same cultivation period, so as to evaluate whether the former is indeed better than the standard expected level and meets the previously set threshold judgment indicators.

[0052] Next, the specific evaluation method of the present invention during field experiments is described. In the process of field experiment evaluation, a control area containing multiple temperature gradients is first set up. This measure is intended to simulate the specific growth performance of plants under different climatic environments. By creating different temperature gradient areas, it is possible to test whether crops have better survival adaptability when facing a changing external environment. For example, in one embodiment, the test field can be divided into several test areas, which are controlled to normal temperature (about 25 degrees Celsius), slightly higher (30 degrees Celsius) and higher temperature (such as 35 to 40 degrees Celsius); Comparing the efficacy of compound substances based on plant growth indicators measured at different treatment levels means recording and comparing the specific growth status of crops in each set temperature zone under untreated conditions (i.e. as a control group) and after adding specific compound substances. Specifically, it is to measure and collect data on changes in key physiological parameters between the same type of crops that have been treated with substances that have the potential to enhance crop heat resistance and those that have not been treated in each temperature zone, so as to compare the relative differences and significance of the effects between the two. If a comparative analysis is performed in a certain experimental design by setting the crop growth rate L under normal conditions and the new plant growth rate R obtained after treatment, the product performance can be more intuitively displayed.

[0053] If **under high temperature S, the plant growth rate R after adding the compound is significantly increased compared to the growth rate L under natural conditions. According to the standards set in the article, the conditions here refer to the application of the compound in a relatively extreme hot environment can at least increase the plant growth rate by 1.5 times relative to the natural situation. This is expressed by the calculation formula R / L to express the ratio of the plant growth rate after compounding to the original state. In the formula, R refers to the growth rate measured after the functional materials are added to the crop, and L represents the natural growth rate without any additional intervention. This ratio is set at ≥1.5 to meet the minimum standard. Such a standard is set because it is necessary to verify the effective effect of the additive under environmental pressure, especially when facing rising temperature stress. For example, after a certain compound with high temperature resistance is applied to a specific plant, its yield or growth is significantly improved compared with the control area under the experimental high temperature environment, which is enough to prove the potential and value of this method.

[0054] The experimental process in the present invention is: 1. Plant extract screening and identification experiment Objective: To screen out natural plant extracts with strong antioxidant capacity.

[0055] Methods: A variety of bioactive compounds were extracted from plants such as green tea, pomegranate peel, and black ginger root. The free radical scavenging experiment was used to test the antioxidant activity of these compounds in vitro. The DPPH method and ABTS method were used as the main detection methods to determine the free radical scavenging ability of different extracts.

[0056] Results: The catechins and ellagic acid rich in green tea and pomegranate peel extracts showed strong antioxidant activity with low IC50 values, and were suitable as the main ingredients in subsequent experiments.

[0057] 2. Large-scale fermentation production of antioxidant ingredients Objective: To mass produce the selected antioxidant ingredients using microbial fermentation technology.

[0058] Methods: Saccharomyces cerevisiae was selected as the production strain, the optimal growth conditions (temperature 30°C, pH 5.5) were set, and inducers were added to induce it to produce metabolites rich in catechins and ellagic acid. The fermentation products were extracted after 48 hours of cultivation, and highly effective antioxidants were obtained after concentration and purification.

[0059] Results: Under standard fermentation conditions, Saccharomyces cerevisiae produced high yields of catechins and ellagic acid. Slight adjustments to the fermentation conditions did not significantly increase the yields, so standard conditions were suitable for large-scale production.

[0060] 3. Experiment on compounding trace elements and antioxidant ingredients Experimental purpose: To screen out the best combination of trace elements and antioxidant ingredients.

[0061] Experimental materials: Different concentrations of zinc (Zn), manganese (Mn), iron (Fe) and antioxidants (catechins, ellagic acid).

[0062] Detection indicators: Determine the activities of catalase (CAT) and superoxide dismutase (SOD) in crops.

[0063] Analysis of results: The combination of Group 2 (Zn 0.2 mg / L, Mn 0.1 mg / L, Fe 0.8 mg / L, antioxidant 8 mg / L) significantly increased the activities of CAT and SOD, and was the combination with the strongest antioxidant capacity.

[0064] 4. Antioxidants and trace elements carrier packaging experiment Purpose of the experiment: To protect antioxidant ingredients through carrier encapsulation technology and evaluate their stability in high temperature environment.

[0065] Carrier material: sodium alginate.

[0066] Test method: Stability test of the encapsulated particles at different temperatures (time to release antioxidants).

[0067] Analysis of results: Sodium alginate-coated particles showed a longer release time of antioxidants under high temperature conditions, ensuring a long-lasting effect.

[0068] 5. Field application experiment Experimental purpose: To verify the application effect of the compound on rice crops under high temperature environment.

[0069] Experimental conditions: The experimental group was treated with a high temperature resistant compound, while the control group was not treated. The field temperature was 35-40°C, and the application rate of the experimental group was 3 kg / ha.

[0070] Analysis of results: In the experimental group, the plant height and leaf area of ​​rice increased significantly, the photosynthetic rate increased by 15%, the antioxidant enzyme activity increased significantly, and the MDA content decreased, indicating that the compound effectively improved the antioxidant capacity and growth state of rice under high temperature environment, and finally increased the yield by 25%.

[0071] The method for preparing a product with crop resistance to high temperature of the present invention comprises: first, screening and identifying effective antioxidant components by conducting in-depth research on natural plant extracts. This step aims to identify compounds with strong anti-adversity properties from a large number of plant extracts. Subsequently, after confirming the effectiveness of these antioxidants, these antioxidant components are efficiently mass-produced using modern microbial fermentation technology. This not only solves the problem of unstable yield in traditional processes, but also ensures high consistency of product quality and economic cost-effectiveness.

[0072] In order to further enhance the ability of crops to resist high temperatures, after completing the key components of fermentation production, the method also includes a unique formula preparation step: mixing the obtained antioxidant components with trace minerals that must be taken in during their growth, so as to improve the overall robustness of the crops. Then, a special coating technology is used to fix the formula on a carrier material with good stability to protect the above-mentioned biologically active components from damage, thereby ensuring that the effect can be maintained for a long period of time after application. When applied to crops, the method of the present invention can establish a protective layer structure on the leaf surface.

[0073] Specifically, the raw material composition includes effective ingredients such as trehalose, which can improve the tolerance of crops to adverse environmental factors, such as high temperature stress, by promoting the operation of the antioxidant system. In particular, the method of converting starch into trehalose, which is more drought-resistant and heat-resistant, through specific strains is not only innovative but also very scientific. The entire technical process effectively avoids the imbalance of the plant's antioxidant enzyme system and the subsequent physiological damage caused by the continuous rise in temperature. In summary, this new approach of comprehensive application of biotechnology and physical protection methods shows great potential in improving crop resistance to extreme climates, while providing a more sustainable development path to help farmers ensure stable crop yields.

[0074] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for producing a product with crop high temperature resistance function, characterized in that: The method comprises: S1, based on screening and identification of antioxidant components in natural plant extracts; S2, producing the antioxidant component by using microbial fermentation technology; S3, compounding the antioxidant component with trace elements required for crop growth; S4, encapsulating the compound based on a heat-stable carrier material to protect the biological activity of the antioxidant component; S5. Applied to crops, it forms a protective film on the surface of crops, increases the antioxidant enzyme activity of crops, and reduces cell damage; Wherein, the extract in step S1 is made of the following raw materials in parts by weight: 100-150 parts of trehalose, 5-10 parts of mineral source fulvic acid, 50-100 parts of complex sugar alcohol, 300-400 parts of calcium acetate, 1-5 parts of ectoine, and 400-500 parts of distilled water; The trehalose production process is as follows: starch hydrolysate is converted into trehalose by utilizing the action of maltooligosaccharide, trehalose synthase and maltooligosaccharide-based trehalose hydrolase.

2. The method for producing a product with crop high temperature resistance function according to claim 1, characterized in that: The step S3 of compounding the antioxidant component with trace elements required for crop growth comprises: Identify the effects of specific trace element combinations on plant enzyme systems; Identify the optimal addition amounts of trace elements and their mutual ratios; Compounding is done based on the determination of the synergistic effect of trace elements on antioxidant components; The final proportion formula is determined based on the interaction mechanism between specific elements.

3. The method for producing a product with crop high temperature resistance function according to claim 2, characterized in that: The process of identifying the optimal addition amount of trace elements and their mutual ratios includes: Select at least two candidate trace elements, and preliminarily determine the experimental interval according to their usual content range in nature, assuming that candidate element a is A and candidate element b is B; Test the effects of various elements at different concentration gradients on the stress resistance of target plants; Screen out the optimal A and B concentrations that significantly improve the target enzyme activity; Based on the maximization of the activity value of the target enzyme, the ideal relative concentration ratio between A and B was analyzed by linear regression.

4. The method for producing a product with crop high temperature resistance function according to claim 3, characterized in that: The process of analyzing the ideal relative concentration ratio between A and B by linear regression includes: Collect the enzyme activity data corresponding to the mixture of elements A and B obtained from different experimental batches; Draw a curve fitting graph to show the relationship between A and B, and find the best fit point; Set the theoretical value Y that b should reach when a is X, where X represents a specific amount of element A and Y represents the theoretical amount of element B that needs to be added. The optimal ratio that meets the above calculation requirements is recorded and used for further tests to verify the performance of the antioxidant composition.

5. The method for producing a product with crop high temperature resistance function according to claim 4, characterized in that: The antioxidant composition performance verification process in the further test includes: Predict the effect of the new ratio scheme in plants based on the established mathematical model; Indoor simulation test to verify whether the actual effect of the prediction scheme meets the expected standards; If the test results show that the improved ratio significantly improves the target enzyme activity level, it will enter the field trial stage; In the actual crop environment, the effectiveness of the final optimized version is tested through comparative experiments, and adjustments are made to make it more adaptable to natural conditions.

6. The method for producing a product with crop high temperature resistance function according to claim 5, characterized in that: The effect of the new ratio scheme in plants predicted based on the established mathematical model includes: Import the experimental data into the established mathematical equation, that is, when the input variable E changes, the response variable D will change by ΔD / E=Z, where E represents the change factor under external high temperature conditions; The above formula is used to simulate the ability of the new formulation to resist adverse environment; If the ΔD / E ratio is low or close to the theoretical optimum, the model is considered reliable and can be applied to future R&D designs; If the value is too large, you need to continue to modify the formula.

7. The method for producing a product with crop high temperature resistance function according to claim 5, characterized in that: The field experiment condition judgment rule is: when the crop is subjected to external extreme temperature stimulation T, the content of protective substances F in the plant increases by G in the state with added compound liquid compared with that without compound liquid, then it is considered that the anti-temperature damage effect is enhanced, where T refers to the high temperature intensity in the external environment, F refers to the mass of specific antioxidants in the cells obtained by detection, and G represents the increase in F.

8. The method for producing a product with crop high temperature resistance function according to claim 5, characterized in that: The specific evaluation methods during the field experiment include: Establish control areas containing multiple temperature gradients; The efficacy of the compound was compared based on the plant growth indices measured under different treatment levels, and the growth rate L under normal conditions was compared with the growth rate R after treatment for comparative analysis; If under high temperature S, the R of the plant after adding the compound is at least 5 times higher than that of the normal growth L (R / L≥1.5), it is considered that this solution effectively improves temperature resistance.

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