Abelmoschus manihot leaf extract and application thereof

Abstract

The invention discloses an abelmoschus manihot leaf extract and application thereof, and belongs to the technical field of abelmoschus manihot leaf extracts. The invention provides an application of an abelmoschus manihot leaf ethanol extract in preparation of a product for reducing blood sugar. The invention further provides application of the abelmoschus manihot leaf water extract in preparation of hair-blacking products. The invention also discloses an application of the abelmoschus manihot leaf extract in preparation of a product with antioxidant activity. According to the method, an Abelmoschus manihot leaf water extract, an Abelmoschus manihot leaf 95% ethanol extract and an Abelmoschus manihot leaf ethyl acetate extract are extracted, and the DPPH and ABTS free radical scavenging rate, the FRAP total reducing capacity and the influence of the extracts on the activity of alpha-glucosidase and tyrosinase are measured. Results show that the Abelmoschus manihot leaves have good antioxidant activity, GLE can be used as a hypoglycemic drug, and GLW can be used for developing related products for increasing melanin. The invention provides a reference basis for comprehensive development and product application of the abelmoschus manihot leaves.

Description

Technical Field

[0001] The invention belongs to the technical field of helianthus annuus leaf extract, and in particular relates to helianthus annuus leaf extract and application thereof. Background Art

[0002] Diabetes is a global metabolic disease characterized by high blood sugar. Among them, the increase in α-glucosidase activity is one of the key factors leading to increased blood sugar. Therefore, in clinical practice, the use of α-glucosidase inhibitors has become an important treatment for diabetes and its complications. However, common hypoglycemic drugs such as acarbose can control blood sugar to a certain extent, but long-term use may cause gastrointestinal diseases, dizziness, nausea and other side effects, and the price is relatively expensive. Therefore, it is of great significance to seek safe, non-toxic, cheap, efficient and widely used plant-derived natural products or natural inhibitors as alternatives.

[0003] In addition, with the acceleration of the pace of modern society, people are facing increasing pressure in work and life, and many young people have premature gray hair. This is mainly due to the weakening of tyrosinase activity, which leads to a decrease in the synthesis of melanocytes. However, most hair dyes on the market contain heavy metal components, which are harmful to the human body. In this case, the study of tyrosinase activity of golden sunflower leaves as a green and environmentally friendly raw material is particularly important. This can not only provide a new source of raw materials for the development of black hair products, but also meet the modern people's pursuit of health and environmental protection.

[0004] Golden flower sunflower, also known as vegetable hibiscus, its scientific name is hibiscus, belonging to the genus Abelmoschus of the Malvaceae family, is a precious herb and is an annual plant. This plant is known for its strong adaptability and has relatively low requirements for growth conditions, so it is relatively easy to obtain raw materials. Golden flower sunflower is recorded in the Compendium of Materia Medica as a herb with the effects of clearing heat and detoxifying, reducing swelling and relieving pain. Modern medical research has further discovered its important economic research value as a dual-purpose herb. The seeds, fruits, peels, rhizomes, flowers, and leaves of golden flower sunflower are rich in efficacy. The value of golden flower sunflower is not only reflected in its medicinal use. Its roots, stems and leaves can be crushed and screened to make snacks and pasta, and can also be used to soak in water and wine. The flowers of golden flower sunflower contain a large amount of plant hormones, which have a miraculous effect on delaying aging. Therefore, the research and development of golden flower sunflower is generally carried out around the flowers of golden flower sunflower, while the leaves of golden flower sunflower are less studied. Therefore, golden flower sunflower still needs to be further developed and researched. The rational development and utilization of sunflower leaves can provide a reference for the comprehensive development and product application of sunflower leaves. Summary of the invention

[0005] To solve the above technical problems, the present invention proposes a helianthus leaf extract and its application. Helianthus leaf has good antioxidant activity, 95% ethanol extract of helianthus leaf can be used as a hypoglycemic drug, and helianthus leaf water extract can be used to develop related products for increasing melanin.

[0006] The present invention provides the following technical solutions:

[0007] Technical solution 1: Application of an ethanol extract of Helianthus annuus leaves in the preparation of a product for lowering blood sugar.

[0008] Furthermore, the lowering of blood sugar is achieved by inhibiting the activity of α-glucosidase.

[0009] Furthermore, the ethanol extract of Helichrysum chrysantha leaves is prepared by using ethanol with a volume concentration of 95% as an extractant.

[0010] Technical solution 2: Application of a water extract of Helianthus annuus leaves in the preparation of black hair products.

[0011] Furthermore, the dark hair is achieved by increasing melanin.

[0012] Furthermore, the increase of melanin is achieved by activating the activity of tyrosinase.

[0013] Technical solution three: Application of a sunflower leaf extract in the preparation of products with improved antioxidant activity.

[0014] Furthermore, improving the antioxidant activity of the product includes improving the DPPH free radical scavenging rate, ABTS free radical scavenging rate and FRAP total reducing capacity of the product.

[0015] Furthermore, the Helianthus annuus leaf extract includes Helianthus annuus leaf water extract, Helianthus annuus leaf ethanol extract and Helianthus annuus leaf ethyl acetate extract.

[0016] Furthermore, the extractant used for the ethanol extract of Helianthus annuus leaves is ethanol with a volume concentration of 95%.

[0017] Compared with the prior art, the present invention has the following advantages and technical effects:

[0018] The present invention adopts the reflux leaching method to extract the water extract (GLW) of golden sunflower leaves, the 95% ethanol extract (GLE) of golden sunflower leaves, and the ethyl acetate extract (GLEA) of golden sunflower leaves, and determines the contents of total phenols, total flavonoids, total sugars and total triterpenes in the three extracts. The antioxidant activity of the golden sunflower leaf extract was explored by measuring the DPPH, ABTS free radical scavenging rate and FRAP total reducing capacity, and the effect of the golden sunflower extract on the activity of α-glucosidase and tyrosinase was studied. The results showed that the total phenol content and total sugar content of the golden sunflower extract were: GLW>GLE>GLEA, and the flavonoid content and total triterpenoid content were not much different. Golden sunflower leaves have good antioxidant activity, DPPH free radical scavenging rate: GLEA>GLE>GLW, ABTS free radical scavenging rate: GLW>GLEA>GLE, FRAP total reducing capacity: GLW>GLE>GLEA; GLE's inhibitory effect on α-glucosidase IC 50 The value is 0.00120 mg / mL, which is slightly lower than acarbose, so GLE can be used as a hypoglycemic drug. 50 The value is 1.47 mg / mL. Due to the weakening of tyrosinase activity, the synthesis of melanin cells is reduced. GLW can be used to develop products related to increasing melanin. The present invention provides a reference for the comprehensive development and product application of golden sunflower leaves. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0020] Figure 1 is the scavenging rate of DPPH free radicals by the extracts of each treatment group of Helianthus annuus leaves;

[0021] Figure 2 is the scavenging rate of ABTS free radicals by the extracts of each treatment group of Helianthus annuus leaves;

[0022] Figure 3 is the standard curve of ferrous sulfate;

[0023] Figure 4 The effects of the extracts of each treatment group of Helianthus annuus leaves on α-glucosidase;

[0024] Figure 5 is the effect of GLE on the inhibition of α-glucosidase;

[0025] Figure 6The effect of the extracts of each treatment group of Helianthus annuus leaves on tyrosinase activity;

[0026] Figure 7 This is the effect of GLE on tyrosinase activity. DETAILED DESCRIPTION

[0027] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0028] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0029] Unless otherwise indicated, all technical and scientific terms used in the present invention have the same meanings as those generally understood by those skilled in the art. Although the present invention only describes preferred methods and materials, any methods and materials similar or equivalent to those described in the present invention may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0030] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0031] The words “include,” “including,” “have,” “contain,” etc. used in the present invention are open-ended terms, meaning including but not limited to.

[0032] Example 1

[0033] 1. Experimental reagents and methods

[0034] 1. Experimental Reagents

[0035] The experimental reagents used in the present invention are shown in Table 1, and the instruments used in the present invention are shown in Table 2.

[0036] Table 1 Experimental reagents

[0037]

[0038]

[0039] Table 2 Experimental instruments

[0040] instrument model factory Ultrafine pulverizer PLS-10LAN Jinan Pulaishen Machinery Equipment Co., Ltd. Rotary evaporator RE-52AA Shanghai Yarong Biochemical Instrument Factory UV-Vis Spectrophotometer T-6 Beijing Puxi General Instrument Co., Ltd. Microplate incubator WKB-200 Toho Electromechanical Technology (Shanghai) Co., Ltd. Electric constant temperature water bath HH-2 Beijing Kewei Yongxing Instrument Co., Ltd.

[0041] 2. Experimental Methods

[0042] 2.1 Extraction of active ingredients from Helianthus annuus leaves

[0043] The golden sunflower leaves are put into a multifunctional pulverizer and crushed to obtain golden sunflower leaf powder, which is sieved through a 60-mesh sieve, and leached at a constant temperature for 4 hours using ultrapure water, 95% ethanol and ethyl acetate as solvents respectively according to a solid-liquid ratio (g:mL) of 1:10. After cooling to room temperature, suction filtration is performed, leaving the filtrate, and then the filtrate is placed in a rotary evaporator, evaporated and concentrated under reduced pressure at 45°C and 105r / min, and placed in a 50°C drying oven to obtain golden sunflower leaf water extract (GLW), golden sunflower leaf alcohol extract (GLE), and golden sunflower leaf ethyl acetate extract (GLEA), and the extract is placed at room temperature and sealed to avoid light for standby use. The golden sunflower leaf extraction rate (%) refers to the mass percentage of the mass of the dried extract to the total mass of the golden sunflower leaf powder.

[0044] 2.2 Determination of active ingredient content

[0045] (1) Determination of total phenol content

[0046] Reference (Guo Jialing, Chen Yu, Xie Shiting, et al. Research progress of flavonoid hyperoside in Helianthus annuus [J]. Food Safety Guide, 2023, (23): 174-176.), the results (mg / g) were expressed as the equivalent of gallic acid (mg) per gram of extract, and each test was repeated 3 times.

[0047] (2) Determination of total flavonoid content

[0048] Reference (Wang Xin, Liu Yumo, Hao Jing, et al. Optimization of ultrasonic-assisted extraction process and performance of flavonoids from Helianthus annuus [J / OL]. Fine Chemicals, 1-11 [2024-04-24]. https: / / doi.org / 10.13550 / j.jxhg.20230860.), the results were expressed as rutin equivalent (mg) per gram of extract (mg / g), and each test was repeated 3 times.

[0049] (3) Determination of total sugar content

[0050] Reference (Chen XT, Lu J, Li X, et al. Effect of blanching and drying temperatures on starch-related physicochemical properties, bioactive components and antioxidant activities of yam fl flours [J]. LWT-Food Science and Technology, 2017, 82: 303-310.), expressed as the glucose equivalent value (mg) per gram of water extract (mg / g), and each test was repeated 3 times.

[0051] (4) Determination of total triterpenoid content

[0052] Reference (Cai Hongmei, Tian Ziyu. Determination of total sugar content in strawberry by phenol-sulfuric acid method [J]. Jilin Agriculture, 2019, 445(04): 46.), expressed as the oleanolic acid equivalent value (mg) per gram of water extract (mg / g), and each test was repeated 3 times.

[0053] 2.3 In vitro antioxidant activity assay

[0054] (1) DPPH free radical scavenging ability assay

[0055] Preparation of DPPH solution: The concentration is 1.3×10 -4 mol / L DPPH ethanol solution (Sun Shili, Liu Shumei, Zhao Chaoyi, et al. Study on the synergistic antioxidant effect of tea polyphenols and vitamin C / E [J]. Guangdong Agricultural Sciences, 2013, 40(01): 96-98.). Preparation method of reference VC solution: Prepare VC solution to the same concentration as the sample and then store it in the dark.

[0056] Preparation of sample solution: Take 40 mg of GLW, GLE, and GLEA respectively, prepare them into 5 mg / mL solution with anhydrous ethanol and dilute the solution to different multiples for later use.

[0057] Determination of DPPH free radical scavenging rate: Use an ELISA instrument to measure the absorbance at 517 nm, take liquid in a 96-well plate according to Table 3, and react the mixed solution at room temperature in the dark for 30 minutes. Perform three parallel tests, take the average value, and calculate the scavenging rate.

[0058] DPPH free radical scavenging rate (%): = [1-(A i -A j ) / A0]*100%

[0059] In the formula, Ai , A j and A0 are the absorbances of the drug group, ginseng group, and DPPH group-blank group, respectively.

[0060] Table 3 DPPH free radical activity determination method

[0061] Reagents Blank group (μL) DPPH group (μL) Ginseng group (μL) Drug group (μL) Anhydrous ethanol 200 40 160 0 DPPH solution 0 160 0 160 Sample solution 0 0 40 40

[0062] (2) ABTS free radical scavenging ability

[0063] Preparation of ABTS solution: Dissolve ABTS and potassium persulfate in ultrapure water respectively, mix them evenly in a ratio of 1:1 at 4°C and protect from light for 12 h to obtain a blue-green ABTS mother solution, which is then diluted with ultrapure water in the experiment to obtain an ABTS cation free radical application solution until the absorbance at a measured wavelength of 734 nm is 0.70±0.020.

[0064] Sample solution preparation: Take 40 mg of GLW, GLE, and GLEA respectively, prepare them into 8 mg / mL solution with ultrapure water and dilute the solution to different multiples for later use.

[0065] Preparation of reference VC solution: Use ultrapure water with the same concentration as the sample to prepare VC solution and store it away from light.

[0066] Determination of ABTS free radical scavenging rate: Take liquid from a 96-well plate according to Table 4, react the mixed solution in the dark at room temperature for 30 minutes, measure the absorbance at 734 nm with an enzyme marker, calculate the scavenging rate, perform three parallel experiments and take the average value.

[0067] σ

[0068] In the formula, A i , A j and A0 are the absorbances of the drug group, ginseng group, and ABTS-blank group, respectively.

[0069] Table 4 ABTS free radical scavenging ability determination method

[0070] Reagents Blank group (μL) Ginseng group (μL) Drug group (μL) Ultrapure water 50 200 0 ABTS 200 0 200 Sample solution 0 50 50

[0071] (3) Total reducing capacity (FRAP value determination)

[0072] Preparation of FRAP working solution: weigh 5.10g sodium acetate in a 250mL volumetric flask, add 20mL glacial acetic acid, dilute to 250mL with ultrapure water, use sodium hydroxide solution to adjust to pH 3.6 to make sodium acetate buffer; prepare 10mmol / L TPTZ solution with 40mmol / L hydrochloric acid solution; prepare 20mmol / LFeCl3 with ultrapure water; and evenly mix the above three solutions in a ratio of 10:1:1.

[0073] Drawing of standard curve: First, dilute the FeSO4 solution with ultrapure water to obtain a series of solution samples with different mass concentrations. Draw the standard curve of FeSO4 solution, with FeSO4 mass concentration as the horizontal axis and absorbance value as the vertical axis.

[0074] Determination of FRAP clearance rate: The absorbance at 517 nm was measured with an enzyme-labeled instrument, and the total reducing capacity was calculated. Liquids were taken in a 96-well plate according to Table 5, and the mixed solution was reacted at room temperature in the dark for 30 minutes, and the reaction was repeated three times to obtain the average value.

[0075] Table 5 Total reducing capacity determination method

[0076] Reagents Blank group (μL) Ginseng group (μL) Drug group (μL) Ultrapure water 50 150 0 FRAP working solution 150 0 150 Sample solution 0 50 50

[0077] 2.4 α-Glucosidase inhibitory activity assay

[0078] Potassium phosphate buffer (pH=6.8), 1.0μ / mL α-glucosidase solution, and 2.5mmol / LPNPG solution were prepared respectively (Zhang Li, Li Caifang, Li Xiaomei, et al. Inhibitory effect of Canada fleabane α-glucosidase [J]. Journal of Henan University (Medical Edition), 2008, 27(04): 39-41.).

[0079] Preparation of positive control acarbose solution: Prepare an acarbose solution with the same concentration as the sample using ultrapure water.

[0080] Sample solution preparation: Take 40 mg of GLW, GLE, and GLEA respectively, prepare them into 8 mg / mL solution with ultrapure water and dilute the extract solution by half to 0.25 mg / mL.

[0081] The experiments were as follows: blank group, control group, ginseng group, and drug group. Liquids were taken and incubated in a 96-well plate according to Table 6.

[0082] Table 6 α-glucosidase activity determination method

[0083]

[0084] The calculation formula of α-glucosidase inhibition rate is:

[0085] Inhibition rate = 1-(A 药物 -A 药参 ) / (A 空白对照 -A 对照 )×100%

[0086] In the formula, A 药物 For drug group, A 药参 The medicinal ginseng group, A 空白对照 For the blank group, A 对照As the control group.

[0087] 2.5 Tyrosinase activation activity assay

[0088] Prepare 0.05 mol / L phosphate buffer solution, pH = 6.8, dissolve 0.100 g L-DOPA in the phosphate buffer solution and transfer to a 100 mL volumetric flask, dilute to the mark. Then use the above phosphate buffer solution to prepare tyrosinase to 50 μg / mL.

[0089] Sample solution preparation: Take 40 mg of GLW, GLE, and GLEA respectively, and prepare them into 8 mg / mL solution with ultrapure water and dilute the extract solution to 0.25 mg / mL. Take the solution in a 96-well plate according to Table 7 and put it into a 37°C incubator for incubation.

[0090] Table 7 Tyrosinase activity assay

[0091]

[0092]

[0093] Tyrosinase activation rate = (A 药物 -A 药参 ) / (A 对照 -A 空白 )-1×100%

[0094] In the formula, A 药物 For drug group, A 药参 The medicinal ginseng group, A 对照 The control group, A 空白 A blank group.

[0095] 2.5 Data processing

[0096] All tests were performed in parallel three times, and the results were expressed as average values. Excel 2023 software was used for basic data statistics, and Origin 2019 software was used for chart drawing.

[0097] 2. Results

[0098] (1) Analysis of the components of each group of extracts from Helianthus annuus leaves

[0099] Three different solvent extracts of Helianthus annuus leaves were prepared by reflux leaching method and their contents were determined. The results are shown in Table 8. The experiment showed that all three groups were rich in polyphenolic compounds and flavonoid compounds.

[0100] Table 8 Extraction rate of each group of extracts from Helianthus annuus leaves and the contents of total phenols, flavonoids, total sugars and total triterpenes

[0101] sample Extraction rate (%) Total phenols (mg / g) Total flavonoids (mg / g) Total sugar (mg / g) Total triterpenes (mg / g) GLW 14.42 343.63 103.98 188.73 31.47 GLE 13.90 243.74 267.53 132.39 256.43 GLEA 3.78 268.29 270.01 165.16 249.09

[0102] (2) Antioxidant analysis of extracts from Helianthus annuus leaves

[0103] The three groups of extracts from Helianthus annuus leaves all have the ability to scavenge DPPH free radicals, and the ability to scavenge DPPH free radicals gradually increases with the increase of concentration. Figure 1 At a concentration of 1.44 mg / mL, the scavenging rate of GLEA reached 50%. Compared with the other two groups of extracts, GLEA was the first to reach a scavenging rate of 50% at a low concentration. This may be because DPPH free radicals are fat-soluble, and GLEA has the best scavenging effect. IC values ​​of each treatment group and VC 50 The values ​​are shown in Table 9.

[0104] The scavenging rate of ABTS cationic free radicals in Helianthus annuus leaves, the results of the three groups of extracts are as follows Figure 2 As shown in the figure, with the increase of concentration, the scavenging effect of Helianthus annuus leaf extract and ascorbic acid on ABTS cation free radicals also increases. This is because the cation free radical of ABTS is water-soluble, so it has the best cleaning effect on GLW. IC 50 The values ​​are shown in Table 10.

[0105] The FRAP method detects the total reduction degree of the sample. The higher the FRAP absorbance value, the stronger the reduction degree. The absorbance change of the sample is proportional to the content of the reducing substance within a certain range. Figure 3 The FeSO4 standard curve in the equation is y=1.45638x-0.06748, and the correlation coefficient is R 2 =0.99765. The total reducing power of VC was used as a comparison. The reducing power of each treatment group is shown in Table 9.

[0106] Table 9 IC values ​​of in vitro antioxidant activity of extracts from Helianthus annuus leaves 50 Value (mg / mL) and total reducing power (FRAP / mg)

[0107] sample DPPH ABTS FRAP VC 0.0650 0.0170 23.8 GLW 2.42 0.248 0.850 GLE 1.49 0.658 0.210 GLEA 1.44 2.95 0.130

[0108] (3) Inhibitory activity of extracts from Helianthus annuus leaves on α-glucosidase

[0109] As the concentration of Helianthus annuus leaf extract increases, the inhibitory effect on α-glucosidase increases. Figure 4 Since polysaccharides and flavonoids have a very prominent effect on lowering blood sugar levels, it can be shown from the perspective of content that GLE and GLEA have better inhibitory effects on α-glucosidase than GLW. Among them, GLE has the best inhibitory effect, so a smaller concentration treatment is made separately, such as Figure 5 , calculate IC 50The value is 0.00120 mg / mL. In addition, the IC 50 The values ​​were 5.23 mg / mL and 2.51 mg / mL, respectively. Therefore, it can be concluded that the inhibitory effect of the GLE-treated group was more significant.

[0110] (4) Tyrosinase activation activity of various extracts from Helianthus annuus leaves

[0111] Effects of three groups of extracts from Helianthus annuus leaves on tyrosinase activation Figure 6 As shown in Figure 2, when the sample concentration was 0-6 mg / mL, the IC 50 The values ​​were 2.48 mg / mL and 11.49 mg / mL, respectively. When the concentration of GLE reached 1 mg / mL, the activation rate of tyrosinase was consistent with that at high concentrations. For this reason, GLE did the following Figure 7 The results showed that there was no inhibitory effect and the activation rate of tyrosinase was about 20%. The results showed that the extracts obtained by different solvents also had an effect on the activation ability of tyrosinase. It is speculated that when water is used as the solvent, the leaves of the golden sunflower contain fewer impurities, so the activation ability is the best.

[0112] As a natural plant, sunflower has the advantages of rich resources and being green and harmless. In recent years, it has developed into a hot research field. The present invention uses ultrapure water, 95% ethanol, and ethyl acetate as solvents, respectively, and utilizes the reflux leaching method to extract the effective ingredients of sunflower leaves. Studies on sunflower leaf extracts have shown that these extracts contain total phenols, total sugars, total flavonoids, and total triterpenoids. In terms of in vitro antioxidant activity, they all show good efficacy in scavenging DPPH free radicals and ABTS cationic free radicals. In addition, the above extracts also exhibited iron ion reducing antioxidant capacity, further demonstrating the diversity and complexity of their antioxidant effects. Finally, experiments were conducted on the inhibitory effects of various extracts from sunflower leaves on α-glucosidase and the activating effects on tyrosinase activity. It was found that GLE has the best inhibitory ability for α-glucosidase (IC 50 The value is 0.00120mg / mL) is lower than acarbose and can be used as a new hypoglycemic drug. GLW is superior to the other two solvents in terms of tyrosinase activation ability (IC 50 Value is 1.47mg / mL), which can be used as a natural and non-toxic hair darkening product.

[0113] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. Application of an ethanol extract of Helianthus annuus leaves in the preparation of a product for lowering blood sugar.

2. The use according to claim 1, characterized in that: The lowering of blood sugar is achieved by inhibiting the activity of α-glucosidase.

3. The use according to claim 1, characterized in that: The ethanol extract of Helianthus annuus leaves is prepared by using ethanol with a volume concentration of 95% as an extractant.

4. Application of a water extract of Helianthus annuus leaves in the preparation of hair-blackening products.

5. The use according to claim 4, characterized in that: The dark hair is achieved by increasing melanin.

6. The use according to claim 5, characterized in that: The increase in melanin is achieved by activating the activity of tyrosinase.

7. Use of a helianthus annuus leaf extract in the preparation of products with improved antioxidant activity.

8. The use according to claim 7, characterized in that: The improving the antioxidant activity of the product includes improving the DPPH free radical scavenging rate, ABTS free radical scavenging rate and FRAP total reducing capacity of the product.

9. The use according to claim 7, characterized in that: The helianthus annuus leaf extract comprises helianthus annuus leaf water extract, helianthus annuus leaf ethanol extract and helianthus annuus leaf ethyl acetate extract.

10. The use according to claim 9, characterized in that: The extractant used for the ethanol extract of Helianthus annuus leaves is ethanol with a volume concentration of 95%.

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