Nutrient solution for cultivating fragrant rice glutinous rice black rice and application and cultivation method of nutrient solution

Abstract

The invention relates to the technical field of rice planting methods, in particular to a nutrient solution for cultivating fragrant rice glutinous rice black rice and application and a cultivation method of the nutrient solution. Aiming at black grasses, a common rice nutrient solution added with exogenous amino acid and exogenous organic acid can be used, and a common rice nutrient solution subjected to compound treatment by adding phenylalanine with the final concentration of 0.4 mM and salicylic acid with the final concentration of 5 mu M can be added. The nutrient solution for culturing the fragrant rice glutinous rice black rice can be applied to the aspect of improving the rice quality, especially the fragrant rice glutinous rice black rice. According to the nutrient solution for culturing the fragrant glutinous rice'black rice ', the plant height, the biomass (fresh weight), the nitrogen content, the chlorophyll content and the single plant dry weight of the'black rice' can be effectively improved. The problem of poor growth and development of the black grasses in a common nutrient solution can be solved, the adaptability of the black grasses is enhanced to a certain extent, the yield is slightly increased, and the process of building green agriculture is promoted.

Description

Technical Field

[0001] The present application relates to the technical field of rice planting methods, and specifically to a nutrient solution for cultivating fragrant and glutinous rice "black rice" and its application and cultivation method. Background Art

[0002] Rice is one of the most important food crops in the world, providing food for nearly 50% of the world's population. China is a major rice producer and consumer, with two-thirds of the population relying on rice as their staple food. China has long been committed to rice breeding, focusing on improving rice yield and quality.

[0003] Xianghenuo is a special rice ecological group formed by the genetic evolution and co-evolution of Asian cultivated rice in a specific ecological region. It is one of the most mountainous and representative traditional rice resources in Guizhou. There are many varieties of Xianghenuo, some of which still retain the characteristics of wild rice, with excellent traits such as cold resistance, barrenness resistance, drought resistance, waterlogging resistance, and shade resistance. The rice has excellent nutritional quality and a strong aroma (Jiao Aixia. Investigation on the current status of Xianghenuo resources utilization and protection in Dong villages in Liping County, Guizhou [J]. Journal of Plant Genetic Resources, 2015, 16(1): 173-177). Among them, the colored rice variety in Xianghe glutinous rice contains extremely rich nutrients, including protein, starch, vitamins, dietary fiber, etc., which can effectively prevent and reduce the occurrence of diseases such as atherosclerosis, cancer, and allergies. As a special germplasm resource, colored rice is not only important for healthy diet but also for the development of human society (Dong Jiahong, et al. Research progress on nutritional characteristics and functional components of colored rice [J]. Journal of Yunnan Nationalities University (Natural Science Edition), 2023, 32(04): 449-458). These characteristics just meet the requirements of modern agricultural development, so the planting area should be increased.

[0004] However, due to the strong regionality of the black rice in Xianghe glutinous rice and the generally low yield, it is difficult to expand the cultivation of this variety. In addition, it grows poorly and has poor resistance under conventional planting conditions. These problems have greatly hindered the development of large-scale, intensive and factory-based planting of "Heihe".

[0005] Therefore, it is extremely important to explore a planting and cultivation method suitable for cultivating Guizhou's local high-quality specialty rice "Heihe" in order to advance the breeding process of Xianghe glutinous colored rice. Summary of the invention

[0006] In order to solve the above technical problems existing in the prior art, the present application provides a nutrient solution for cultivating fragrant glutinous rice "black rice" and its application and cultivation method, which are as follows:

[0007] A nutrient solution for cultivating fragrant glutinous rice "black rice" contains phenylalanine, salicylic acid or both.

[0008] Furthermore, the final concentration of phenylalanine is 0.1 mM, 0.2 mM, 0.4 mM, 0.6 mM, and 1 mM.

[0009] Furthermore, the final concentration of salicylic acid is 5 μM, 10 μM, 15 μM, 20 μM, and 25 μM.

[0010] Furthermore, the nutrient solution contains both phenylalanine and salicylic acid, specifically 0.4 mM phenylalanine and 5 μM salicylic acid.

[0011] Furthermore, the fragrant glutinous rice "Heihe" is a black rice germplasm resource collected from Congjiang County, Qiandongnan Prefecture. The growth period of "Heihe" is 162 days, the seeds are short and round, the seed shell is slightly brown, the awn rate is low, and the awns are short. The seeds of "Heihe" were collected in Qiandongnan Prefecture, Guizhou Province, and are now stored in the Plant Hormone and Nutrition Molecular Regulation Laboratory of the Rice Industry Technology Research Institute of Guizhou University. They can also be purchased from local companies.

[0012] Furthermore, the nutrient solution also contains the following ingredients: NaH2PO4·2H2O 63.000 mg / L, K2SO4 111.625 mg / L, CaCl2 39.55 mg / L, MgSO4·7H2O 506.250 mg / L, MnCl2·4H2O 1.761 mg / L, Na2MoO4·2H2O 0.127 mg / L, H3BO4 1.168 mg / L, ZnSO4·7H2O 0.044 mg / L, CuSO4·5H2O 0.039 mg / L, EDTA-Na2 15.000 mg / L, and FeSO4·7H2O 11.140 mg / L.

[0013] Furthermore, in the compound nutrient solution to which phenylalanine and salicylic acid were added, the final concentrations were 0.1 mM Phe and 5 μM Salicylic acid, 0.1 mM Phe and 10 μM Salicylic acid, 0.1 mM Phe and 15 μM Salicylic acid, 0.1 mM Phe and 20 μM Salicylic acid, 0.1 mM Phe and 25 μM Salicylic acid, 0.2 mM Phe and 5 μM Salicylic acid, 0.2 mM Phe and 10 μM Salicylic acid, 0.2 mM Phe and 15 μM Salicylic acid, 0.2 mM Phe and 20 μM Salicylic acid, 0.2 mM Phe and 25 μM Salicylic acid, 0.4 mM Phe and 5 μM Salicylic acid, 0.4 mM Phe and 10 μM Salicylic acid, and 0.4 mM Phe and 25 μM Salicylic acid. Phe and 15μM Salicylic acid, 0.4mM Phe and 20μM Salicylic acid, 0.4mM Phe and 25μM Salicylic acid, 0.5mM Phe and 5μM Salicylic acid, 0.5mM Phe and 10μM Salicylic acid, 0.5mM Phe and 15μM Salicylic acid, 0.5mM Phe and 20μM Salicylic acid, 0.5mM Phe and 25μM Salicylic acid, 1mM Phe and 5μM Salicylic acid, 1mM Phe and 10μM Salicylic acid, 1mM Phe and 15μM Salicylic acid, 1mM Phe and 20μM Salicylic acid, 1mM Phe and 25μM Salicylic acid.

[0014] The nutrient solution for cultivating the fragrant glutinous rice "Black Grass" of the present application can be used for applications in improving the quality of rice, especially the fragrant glutinous rice "Black Grass". The nutrient solution for cultivating the fragrant glutinous rice "Black Grass" of the present application can effectively improve the plant height, biomass (fresh weight), nitrogen content, chlorophyll content and dry weight per plant of "Black Grass". The problem of poor growth and development of "Black Grass" in ordinary nutrient solution can be solved, and the adaptability of "Black Grass" can be enhanced to a certain extent, and a small increase in yield can be achieved, which will be conducive to promoting the process of building green agriculture.

[0015] A method for cultivating fragrant glutinous black rice "Heihe", using the nutrient solution for cultivating fragrant glutinous black rice "Heihe" to cultivate rice seedlings in the three-leaf stage to the five-leaf stage, wherein the variety of the rice seedlings is "Heihe".

[0016] Furthermore, the three-leaf stage rice seedlings are obtained by the following method: rice seeds are hydroponically cultured until the first incomplete leaf appears; the water is replaced with ordinary rice nutrient solution, and cultured to the three-leaf stage to obtain three-leaf stage rice seedlings.

[0017] Compared with the prior art, the technical effects created by this application are embodied in:

[0018] (1) Although adjusting the ratio of ammonia nitrogen and nitrate nitrogen in the nitrogen source of rice nutrient solution, adding exogenous amino acids, adding exogenous organic acids, etc. may have a certain impact on the growth state of rice. However, for the colored rice "Heihe", there is no clear method to increase its yield in the prior art, and the inventor can only explore the cultivation method of this rice from scratch. In the experiment, the inventor added 5 different concentrations of phenylalanine and 5 different concentrations of salicylic acid to the ordinary rice nutrient solution to form 10 treatments. At the same time, 5 different concentrations of phenylalanine were mixed with 5 different concentrations of salicylic acid and added to the ordinary rice nutrient solution to form 25 composite treatments, forming a total of 35 treatments, the purpose of which is to screen out the best treatment conditions. Finally, it was found that different purposes can be achieved by adjusting the composition of the nutrient solution. The ordinary rice nutrient solution with a final concentration of 0.4mM phenylalanine and 5μM salicylic acid composite treatment can effectively improve the plant height, biomass (fresh weight), nitrogen content, chlorophyll content and dry weight per plant of "Heihe", which is the optimal treatment method. The solution of this application can solve the problem of poor growth and development of "black rice" in ordinary nutrient solution, enhance the adaptability of "black rice" to a certain extent, and achieve a small increase in yield, which will be conducive to promoting the process of building green agriculture. The "Xianghe Nuo" described in this solution is a high-quality specialty rice in Guizhou, which has been reported in many prior art documents. In addition to the documents mentioned in the background technology, there are also: "Chen Huicha, Li Xiaobing, You Junmei, et al. Overview of research on protection and utilization of Guizhou's special rice variety Xianghe Nuo germplasm resources [J]. Seed, 2023, 42(11): 61-67+2. Tang Wenhua, Jiang Tianzhi, Yang Xiaoyan, et al. Research on the content of Zn·Cr and its physiological function in the original ecological Danzhai selenium rice [J]. Anhui Agricultural Science, 2009, 37(32): 15686-15687." etc.

[0019] (2) The nutrient solution for cultivating the fragrant glutinous rice "Black Grass" of the present application can be used to improve the quality of rice, especially the fragrant glutinous rice "Black Grass". The nutrient solution for cultivating the fragrant glutinous rice "Black Grass" of the present application can effectively improve the plant height, biomass (fresh weight), nitrogen content, chlorophyll content and dry weight per plant of "Black Grass". The problem of poor growth and development of "Black Grass" in ordinary nutrient solution can be solved, and the adaptability of "Black Grass" can be enhanced to a certain extent, and a small increase in yield can be achieved, which will be conducive to promoting the process of building green agriculture.

[0020] (3) This application studies and determines a nutrient solution formula with a certain promoting effect for the fragrant glutinous colored rice "Heihe". When planting "Heihe", using ordinary rice nutrient solution with a final concentration of 0.4mM phenylalanine and 5μM salicylic acid compound treatment can effectively promote the planting effect of "Heihe", especially in terms of the biomass, plant height, nitrogen content and chlorophyll content of the Heihe plants.

[0021] (4) The present application provides a nutrient solution formula and a cultivation method suitable for the growth and development of "Black Grass", which can hopefully solve the problem that "Black Grass" is difficult to plant in other places due to its strong regionality and cannot adapt to the hydroponic cultivation of ordinary rice nutrient solution. It will be beneficial to accelerate the process of factory-based seedling cultivation of "Black Grass", realize the large-scale, intensive, factory-based planting management of "Black Grass" and promote and apply it on a large scale. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 The statistical results and phenotypic pictures of the plant height, fresh weight, root length and dry weight of "Black Grass" under different concentration treatment conditions in the phenylalanine treatment group of Example 1 of the present application are shown (the statistical results are expressed as mean±SD, n=6; the Duncan test was used for significant difference analysis, and lowercase letters are marked above the data. In the same statistical graph, different lowercase letters indicate significant differences, p<0.05; the same lowercase letters indicate the same significance level).

[0023] Figure 2 The statistical results and phenotypic pictures of the plant height, fresh weight, root length and dry weight of the "Black Grass" plants under different concentration treatment conditions in the salicylic acid treatment group of Example 1 of the present application are shown (the statistical results are expressed as mean±SD, n=6; the Duncan test was used for significant difference analysis, and lowercase letters are marked above the data. In the same statistical graph, different lowercase letters indicate significant differences, p<0.05; the same lowercase letters indicate the same significance level).

[0024] Figure 3 , Figure 4 , Figure 5 , Figure 6 , Figure 7The plant height, fresh weight, root length and dry weight statistics and phenotypic pictures of "Black Grass" plants under different concentration treatment conditions in the phenylalanine and salicylic acid combined treatment group of Example 1 of the present application are shown as mean±SD, n=6; Duncan test was used for significant difference analysis, with lowercase letters marked above the data. In the same statistical graph, different lowercase letters indicate significant differences, p<0.05; the same lowercase letters indicate the same significance level).

[0025] Figure 8 This is the test result of the nitrogen percentage content per plant of “Black Grass” in Example 2 of the present application (statistical results are expressed as mean±SD, n=10; **, p<0.01; *, p<0.05).

[0026] Fig. 9 The chlorophyll content test results of “Black Grass” in 3 implementations of this application (statistical results are expressed as mean±SD, n=10; **, p<0.01; *, p<0.05). DETAILED DESCRIPTION

[0027] The technical solution of the present application is further limited below in conjunction with specific implementation methods, but the scope of protection required is not limited to the description.

[0028] Example 1: Effects of different phenylalanine concentration treatments on the growth and development of "Black Grass".

[0029] Rice varieties: The "Heihe" involved in this plan is a black rice variety of "Xianghe Nuo". The rice seeds used as biological materials in subsequent experiments are seeds of the "Heihe" black rice variety of "Xianghe Nuo", a high-quality specialty rice in Guizhou. The growth period of "Heihe" is 162 days, the seeds are short and round, the seed shell is slightly brown, the awn rate is low, and the awns are short. The seeds of "Heihe" were collected in Qiandongnan Prefecture, Guizhou Province, and are now stored in the Plant Hormone and Nutrition Molecular Regulation Laboratory of the Rice Industry Technology Research Institute of Guizhou University.

[0030] Rice seed soaking: Soak the rice seeds in 0.6% dilute nitric acid for 6 to 8 hours.

[0031] Cultivation of rice seeds: After soaking, rinse the seeds with deionized water for 3 to 5 times, divide them into culture dishes, add appropriate amount of purified water, culture them in an incubator at 37°C, and change the water 1 to 2 times a day.

[0032] Hydroponics: After the seeds germinate, wait until their buds grow to 1-2 cm, sow them in 96-well plates and place them in a 4L culture box with clean water for culture until the first incomplete leaf appears. Then, change to ordinary rice nutrient solution and culture to the 3-leaf stage, and then transplant them into a 15L culture pot. Add 12L of ordinary rice nutrient solution to the culture pots, set up 36 culture pots, set 5 culture pots as phenylalanine treatment group, 5 culture pots as salicylic acid treatment group, 25 culture pots as phenylalanine and salicylic acid combined treatment group, and 1 culture pot as normal control group without treatment.

[0033] The formula of ordinary rice nutrient solution is a conventional culture solution in the prior art, as shown in Table 1 (the nitrogen source is not shown, i.e., nitrogen-free ordinary rice nutrient solution) (reference Yoshida S, Fomo DA, Cock JH, et al. Routine procedure for growing rice plants in culture solution. In: Laboratory manual for physiological studies of rice [J]. International Rice Research Institute, 61-66.). After extensive research, the inventors found that the conventional nitrogen-free ordinary rice nutrient solution can meet the needs of rice growth. The ratio in Table 1 can meet the needs of rice growth and is a specific presentation form of nitrogen-free ordinary rice nutrient solution.

[0034] Table 1: Common rice nutrient solution formula

[0035]

[0036]

[0037] Prepare phenylalanine and salicylic acid mother solutions, the concentration of which is 1 mol / L, for the preparation of nutrient solutions. The specific preparations of the four groups in hydroponics (normal nutrient solution control group, phenylalanine treatment group, salicylic acid treatment group, and phenylalanine and salicylic acid combined treatment group) are as follows:

[0038] Normal nitrogen treatment group: just add 12L of ordinary nutrient solution.

[0039] Phenylalanine treatment group: Add corresponding amounts of mother solution to 12L of ordinary culture medium in 5 culture pots of phenylalanine treatment group to make the concentrations in Table 2. For example, add 1.2ml, 2.4ml, 4.8ml, 7.2ml, and 12ml of phenylalanine mother solution to five culture pots T1, T2, T3, T4, and T5, respectively, so that the concentrations of phenylalanine in the five culture pots are 0.1mM, 0.2mM, 0.4mM, 0.6mM, and 1mM, respectively.

[0040] Salicylic acid treatment group: Add corresponding amounts of mother solution to 12L of ordinary culture medium in 5 culture pots of salicylic acid treatment group to make the concentrations in Table 2. For example, add 60μL, 120μL, 180μL, 240μL, and 300μL of phenylalanine mother solution to five culture pots T1, T2, T3, T4, and T5, respectively, so that the concentrations of phenylalanine in the five culture pots are 5μM, 10μM, 15μM, 20μM, and 25μM, respectively.

[0041] Phenylalanine and salicylic acid combined treatment group: Add corresponding amounts of mother solution to 12L of ordinary culture solution in 25 culture pots of the combined treatment group to prepare the concentrations in Table 2. For example, 1.2ml of phenylalanine mother solution and 60μL of salicylic acid mother solution, 1.2ml of phenylalanine mother solution and 120μL of salicylic acid mother solution, 1.2ml of phenylalanine mother solution and 180μL of salicylic acid mother solution, 1.2ml of phenylalanine mother solution and 240μL of salicylic acid mother solution, 1.2ml of phenylalanine mother solution and 300μL of salicylic acid mother solution, 2.4ml of phenylalanine mother solution and 60μL of salicylic acid mother solution were added to 25 culture pots. , 2.4 ml of phenylalanine stock solution and 120 μL of salicylic acid stock solution, 2.4 ml of phenylalanine stock solution and 180 μL of salicylic acid stock solution, 2.4 ml of phenylalanine stock solution and 240 μL of salicylic acid stock solution, 2.4 μM phenylalanine stock solution and 300 μL of salicylic acid stock solution, 4.8 ml of phenylalanine stock solution and 60 μL of salicylic acid stock solution, 4.8 ml of phenylalanine stock solution and 120 μL of salicylic acid stock solution, 4.8 ml of phenylalanine stock solution and 180 μL of salicylic acid stock solution, 4.8 ml of phenylalanine stock solution and 240 μL of salicylic acid stock solution, 4.8 ml of phenylalanine stock solution and 300 μL of salicylic acid stock solution, 7.2 ml of phenylalanine stock solution and 60 μL of salicylic acid stock solution, 7.2 ml of phenylalanine stock solution and 120 μL of salicylic acid stock solution, 7.2 ml of phenylalanine stock solution and 180 μL of salicylic acid stock solution, 7.2 ml of phenylalanine stock solution and 240 μL of salicylic acid stock solution, 7. 2 ml of phenylalanine stock solution and 300 μL of salicylic acid stock solution, 12 ml of phenylalanine stock solution and 60 μL of salicylic acid stock solution, 12 ml of phenylalanine stock solution and 120 μL of salicylic acid stock solution, 12 ml of phenylalanine stock solution and 180 μL of salicylic acid stock solution, 12 ml of phenylalanine stock solution and 240 μL of salicylic acid stock solution, 12 ml of phenylalanine stock solution and 300 μL of salicylic acid stock solution, so that the final concentration in 25 culture pots is 0.1 mM respectively. Phe and 5μM Salicylic acid, 0.1mM Phe and 10μM Salicylic acid, 0.1mM Phe and 15μM Salicylic acid, 0.1mM Phe and 20μM Salicylic acid, 0.1mM Phe and 25μM Salicylic acid, 0.2mM Phe and 5μM Salicylic acid, 0.2mM Phe and 10μM Salicylic acid, 0.2mM Phe and 15μM Salicylic acid, 0.2mM Phe and 20μM Salicylic acid, 0.2mM Phe and 25μM Salicylic acid, 0.4mM Phe and 5μM Salicylic acid, 0.4mM Phe and 10μM Salicylic acid, 0.4mM Phe and 15μM Salicylic acid, 0.4mM Phe and 20μM Salicylic acid, 0.4mM Phe and 25μM Salicylic acid, 0.5mM Phe and 5μM Salicylic acid, 0.5mM Phe and 10μM Salicylic acid, 0.5mM Phe and 15μM Salicylic acid, 0.5mM Phe and 20μM Salicylic acid, 0.5mM Phe and 25μM Salicylic acid, 1mM Phe and 5μM Salicylic acid, 1mM Phe and 10μM Salicylic acid, 1mM Phe and 15μM Salicylic acid, 1mM Phe and 20μM Salicylic acid, 1mM Phe and 25μM Salicylic acid.

[0042] The treatment methods of the normal hydroponic treatment group, the phenylalanine treatment group, the salicylic acid treatment group, and the phenylalanine and salicylic acid combined treatment group are shown in Table 2:

[0043] Table 2: Preliminary screening treatment (Note: Phe in the table is phenylalanine, and Salicylic acid is salicylic acid, unit: mM)

[0044]

[0045]

[0046] After all the treatments of "Black Grass" have grown to the 5-leaf stage, the plant height, fresh weight, root length and dry weight of the rice seedlings of various treatments were counted. One seedling with a plant height close to the average plant height was selected from each treatment of the normal nitrogen control group and the organic nitrogen treatment group and the inorganic nitrogen treatment group, and the seedlings were evenly spread out on the black cloth in a row. The plant phenotype, fresh weight, dry weight, plant height and root length of "Black Grass" under the treatment conditions of the phenylalanine treatment group are shown in the figure below. Figure 1 A, 1B, 1C, 1D, 1E; the plant phenotype, fresh weight, dry weight, plant height, and root length of "Heihe" under the treatment conditions of salicylic acid are shown in Figure 2 A, 2B, 2C, 2D, 2E; the plant phenotype, fresh weight, dry weight, plant height, and root length of "Heihe" under the combined treatment of phenylalanine and salicylic acid are shown in Figure 3-Figure 7 As shown in A, B, C, D, and E.

[0047] according to Figure 1 The experimental results show that in the phenylalanine treatment group, when phenylalanine was added alone, the plant height, fresh weight, dry weight and root length of the "black grass" plants showed different degrees of inhibitory effects, and showed a downward trend compared with the control. Especially when the phenylalanine concentration reached 1mM, it showed a very obvious inhibitory effect on the growth and development of "black grass", such as Figure 1 B, 1D, 1E.

[0048] according to Figure 2 The experimental results show that in the salicylic acid treatment group, the treatments with 10μM salicylic acid, 15μM salicylic acid and 25μM salicylic acid added to the ordinary nutrient solution had a more significant effect on promoting the growth and development of "Black Grass", among which the most significant was the treatment with 25μM salicylic acid. By weighing them accordingly, it was found that in the treatment groups with 10μM salicylic acid, 15μM salicylic acid and 25μM salicylic acid, the fresh weight and dry weight of "Black Grass" were higher than those of the control group, and the difference in dry weight reached a significant difference, such as Figure 2 C; and the plant height of "Heihe" in the 25μM salicylic acid treatment group was higher than that of the control, reaching a significant difference, such as Figure 2 D; In addition, the root length of "Heihe" in the treatment group of 15μM salicylic acid was higher than that of the control, as shown in Figure 2 E, and reached a significant difference.

[0049] according to Figure 3-Figure 7 The experimental results show that in the combined treatment group of phenylalanine and salicylic acid, the treatments that can significantly promote the plant height and fresh weight of "Black Grass" at the same time and the promoting effect is more obvious are the treatment of adding 0.2mM phenylalanine and 20μM salicylic acid to the ordinary nutrient solution, the treatment of adding 0.2mM phenylalanine and 25μM salicylic acid to the ordinary nutrient solution, the treatment of adding 0.4mM phenylalanine and 5μM salicylic acid to the ordinary nutrient solution, the treatment of adding 0.4mM phenylalanine and 10μM salicylic acid to the ordinary nutrient solution, the treatment of adding 0.4mM phenylalanine and 15μM salicylic acid to the ordinary nutrient solution, and the treatment of adding 0.4mM phenylalanine and 25μM salicylic acid to the ordinary nutrient solution. Moreover, the dry weight under these five treatments is also significantly higher than that of the control treatment as shown in the figure. Figure 4 B, 4D, 5B, and 5D; when the concentration of phenylalanine exceeds 1mM, it is combined with salicylic acid to achieve a significant inhibitory effect on the growth of black rice. Through the test of physiological indicators, it was found that adding 1mM phenylalanine and more than 15μM salicylic acid to the ordinary rice culture medium had the most serious inhibitory effect on "black rice", as shown in Figure 5B. Figure 7 shown.

[0050] The above results show that in the phenylalanine treatment group, the exogenous addition of phenylalanine alone on the basis of ordinary rice nutrient solution will inhibit the growth and development of "Gou Nen Gu", resulting in its poor growth and development; in the salicylic acid treatment group, the treatment of adding 10μM salicylic acid to the ordinary nutrient solution is more conducive to the growth and development of "Heihe"; in the composite treatment, adding ordinary rice nutrient solution with a final concentration of 0.4mM phenylalanine and 5μM salicylic acid to the ordinary rice nutrient solution can effectively increase the plant height, biomass (fresh weight), nitrogen content, chlorophyll content and dry weight per plant of "Heihe", which is the optimal treatment method.

[0051] Example 2: Effects of different treatments on the percentage of total nitrogen content in rice of "Black Grass"

[0052] The materials of rice in this example are all from the above-mentioned culture materials. Nitrogen is the mineral element with the largest demand for plants and is also called the "element of life". When nitrogen is sufficient, plants can synthesize more protein, promote cell division and enlargement, plant leaf area grows faster, and photosynthesis is stronger. When nitrogen is severely deficient, the synthesis of organic matter is blocked, the plants are short, the leaves turn yellow, and the old leaves are more yellow. Therefore, the nitrogen content in the plants can be used as a physiological indicator to judge the growth and development of the plants. The method for measuring the nitrogen content in rice plants is carried out with reference to the literature (Edited by Nanjing Agricultural College. Soil Agrochemical Analysis. Beijing: Agricultural Press, 1980.193-197).

[0053] Rice plant sampling: From the above-mentioned organic nitrogen treatment group and inorganic nitrogen treatment group, the treatments with more obvious promotion and inhibition on the growth and development of "Black Grass" were selected, plus the control group, a total of 7 treatments. Three rice plants were taken from each treatment and placed in an 80°C oven to dry overnight. After that, the dried plants were taken out and divided into leaves and stems. The samples were crushed into powder with a grinder for later use.

[0054] Determination of nitrogen content in plants:

[0055] 1. Digestion:

[0056] 1. Use a 1 / 10,000 balance to weigh 0.5 g of sample (dry sample) and place it in the digestive tube.

[0057] 2. Use a 1:1 balance to weigh 0.5 g of copper sulfate pentahydrate and 4.5 g of potassium sulfate and place them in the digestive tube.

[0058] 3. Use a pipette to accurately draw 8 ml of concentrated sulfuric acid into the digestive tube.

[0059] 4. Turn on the instrument and start digestion. First, digest at 280℃ for about 20 minutes, then change to 350℃ for about 20 minutes, and finally change to 450℃ for digestion. Wait until the liquid in the digestion tube turns into emerald green and digest for another 30-40 minutes. Digestion is complete.

[0060] 5. Allow to cool at room temperature for later use.

[0061] 2. Distillation and titration: The sample solution digested above needs to be diluted to 100ml with distilled water. Pipette 5ml of the test solution into the long glass tube of the nitrogen analyzer, and prick 5ml of the boric acid indicator solution into a wide-mouthed triangular flask. After distillation for 3 minutes, remove it. Titrate with a semi-micro burette, and the titration changes from green to white to pink. Calculate the nitrogen content in the plant according to the following formula using the data obtained.

[0062] Total nitrogen in the plant (%) = equivalent concentration of H2SO4 standard solution × (volume of H2SO4 standard solution consumed when titrating the sample - volume of H2SO4 standard solution in the blank test) × fractionation times × 0.014 × 100 / sample weight

[0063] In the formula: the multiple of taking according to the above method is 20, because 5 ml is taken out of 100 mL, and the sample weight is 0.5 g. Finally, the average nitrogen content of the plants measured in the three repeated experiments for each treatment is calculated.

[0064] The nitrogen content data of each treatment sample was statistically plotted, and the results are as follows: Figure 8 As shown in the figure, the above results once again show that in the organic nitrogen treatment group, exogenous application of 0.4mM Phe and 5μM Salicylic acid on the basis of ordinary nutrient solution can promote the growth and development of "Black Grass" and improve its poor growth and development problem in ordinary nutrient solution.

[0065] Example 3 Effect of exogenous application of phenylalanine and salicylic acid on the chlorophyll content in "Black Grass" leaves.

[0066] The rice materials in this example are all from the above-mentioned culture materials. The chlorophyll content of rice leaves is related to the photosynthetic rate and crop growth rate. In scientific research, chlorophyll content is often measured to characterize the growth status of rice. In production, leaf color changes are often used as an important indicator for seedling diagnosis and fertilizer and water measures. The method for measuring the chlorophyll content in rice leaves is carried out according to the literature (Nurkaimaier Murati, Yang Yajie, Parhat Abdukerimu, et al. Comparison of methods for determining chlorophyll content in wheat [J]. Jiangsu Agricultural Science, 2021, 49(09): 156-159).

[0067] Rice leaf material: Select three rice seedlings from the above treatment group (T1 is the control), take the sword leaf part, cut the leaves except the veins into pieces, and mix them evenly. Weigh 0.2-0.3g of leaf fragments, put them in a 15ml centrifuge tube, mark the leaf weight and add 10ml of anhydrous ethanol, repeat the experiment for 3 groups, and take the average value. Wrap all centrifuge tubes containing rice leaf fragments and anhydrous ethanol with tin foil, store in the dark for 3 days, and shake them several times during the period.

[0068] Determination of chlorophyll content: After 3 days, obtain the chlorophyll extracts of all samples. Prepare 8 cuvettes, add 4 ml of anhydrous ethanol to one of them as a control, and add 3 sets of repeated chlorophyll extracts of each treated sample to the remaining 7 cuvettes. Determine the absorbance values ​​at 663nm and 645nm respectively. Calculate the chlorophyll content using the following formula based on the obtained data.

[0069] Total chlorophyll = (20.21D645 nm + 8.02D 663 nm) × V / 1000 × m

[0070] Where: D663 nm and D645 nm are absorbance at 663 and 645 nm respectively; V is the volume of the test solution (ml); m is the mass of the fresh leaf sample (g) or the leaf area (cm2). Finally, the total amount of chlorophyll measured by the three repeated experiments for each treatment was averaged.

[0071] The measured chlorophyll content was statistically plotted, and the results were as follows: Fig. 9 As shown. On the basis of the ordinary nutrient solution for rice, phenylalanine and salicylic acid were added to make the final concentration reach 0.4mM Phe and 5μM Salicylic acid, and the total chlorophyll content of "Black Grass" was significantly improved compared with the control. The chlorophyll content in the leaves is an important indicator to measure the nutritional status of plants. A high total chlorophyll content represents a stronger photosynthesis potential and can synthesize more nutrients required for plant growth and development. This once again confirms that changing the nutrient solution formula can greatly improve the growth and development of "Black Grass" in the fragrant grass variety, solve the problem of poor growth and development in ordinary nutrient solution that makes it difficult to raise seedlings in factories, and accelerate the large-scale, intensive, and factory-based planting and management of high-quality colored rice varieties against the background of the gradual loss of rural labor.

[0072] The above embodiments are only representative examples of the present application. Obviously, the technical solution of the present application is not limited to the above embodiments, and there are many variations. All variations that can be directly derived or associated with the content disclosed in the present application by a person of ordinary skill in the art should be considered as the protection scope of the present application.

Claims

1. A nutrient solution for cultivating fragrant glutinous rice "black rice", characterized in that: It contains phenylalanine, salicylic acid, or both.

2. The nutrient solution for cultivating fragrant glutinous rice "black rice" according to claim 1, characterized in that: The final concentration of phenylalanine is 0.1 mM, 0.2 mM, 0.4 mM, 0.6 mM, and 1 mM.

3. The nutrient solution for cultivating fragrant glutinous rice "black rice" according to claim 1, characterized in that: The final concentration of salicylic acid is 5 μM, 10 μM, 15 μM, 20 μM, and 25 μM.

4. The nutrient solution for cultivating fragrant glutinous "black grass" according to claim 1, characterized in that: It also contains phenylalanine and salicylic acid.

5. The nutrient solution for cultivating fragrant glutinous "black grass" according to claim 4, characterized in that: The nutrient solution contains 0.4 mM phenylalanine and 5 μM salicylic acid.

6. The nutrient solution for cultivating fragrant glutinous rice "black rice" according to claim 1, characterized in that: The Xianghenuo "Heihe" is a black rice germplasm resource of "Xianghenuo" collected in Congjiang County, Qiandongnan Prefecture.

7. The nutrient solution for cultivating fragrant glutinous rice "black rice" according to claim 1, characterized in that: It also contains the following ingredients: NaH2PO4·2H2O 63.000mg / L, K2SO4 111.625mg / L, CaCl2 39.55 mg / L, MgSO4·7H2O 506.250mg / L, MnCl2·4H2O 1.761mg / L, Na2MoO4·2H2O 0.127mg / L, H3BO4 1.168 mg / L, ZnSO4·7H2O 0.044mg / L, CuSO4·5H2O 0.039mg / L, EDTA-Na2 15.000mg / L, and FeSO4·7H2O 11.140mg / L.

8. Use of the nutrient solution for cultivating fragrant glutinous rice "black rice" as claimed in claim 1 in improving rice quality.

9. A method for cultivating fragrant glutinous black rice "Heihe", characterized in that: The nutrient solution for cultivating the fragrant glutinous rice "Heihe" as claimed in claim 1 is used to cultivate rice seedlings in the three-leaf stage to the five-leaf stage, and the variety of the rice seedlings is "Heihe".

10. The method according to claim 9, characterized in that The three-leaf rice seedlings are obtained by the following method: the rice seeds are hydroponically cultured until the first incomplete leaf appears; the water is replaced with a common rice nutrient solution, and the seeds are cultured until the three-leaf stage, thereby obtaining the three-leaf rice seedlings.

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