Application of basic CuMoO4 nanosheet in prevention and treatment of sclerotinia rot of colza

Abstract

The invention belongs to the field of antibiosis, and discloses application of a basic CuMoO4 nanosheet in prevention and treatment of sclerotinia rot of colza. According to the method, the alkaline CuMoO4 nanosheet is used for preventing and treating the sclerotinia rot of the oilseed rape, so that the yield and income of the oilseed rape are increased.

Description

Technical Field

[0001] The invention belongs to the field of antibacterial, and more specifically, relates to the application of basic CuMoO4 nanosheets in the prevention and treatment of rapeseed sclerotinia disease. Background Art

[0002] The correct cultivation methods of rapeseed, beans and other major crops are essential to ensure food and oil security. In recent years, the occurrence of various extreme events has affected the international trade of oil crops to a certain extent. In addition, under the influence of various diseases, the yield of oil crops has been seriously affected by sclerotinia, downy mildew and bacterial wilt. Scientists from all over the world are also committed to developing new materials for antifungal bacterial infection for oil crops and reducing the harm of antifungal pesticides to the environment, livestock and human health.

[0003] The development of metal or metal oxide nanoparticles has become a cutting-edge area of ​​antifungal research. Since 1939, when molybdenum was identified as one of the essential trace elements for plants, the antiviral properties of molybdate compounds have become a focus of research. Summary of the invention

[0004] The purpose of the present invention is to address the defects of the prior art and propose the application of basic CuMoO4 nanosheets in the prevention and treatment of rapeseed sclerotinia. The present invention uses basic CuMoO4 nanosheets to prevent and treat rapeseed sclerotinia, thereby increasing the yield and income of rapeseed.

[0005] In order to achieve the above object, the present invention provides the use of basic CuMoO4 nanosheets in the prevention and treatment of rapeseed sclerotinia disease.

[0006] According to the present invention, preferably, the basic CuMoO4 nanosheets are mixed with water to obtain an antifungal agent for controlling rapeseed sclerotinia.

[0007] According to the present invention, preferably, the control method comprises: spraying the antifungal agent at the early flowering stage of rapeseed, or spraying the antifungal agent at the early flowering stage and the pod-setting stage of rapeseed in sequence.

[0008] According to the present invention, preferably, when spraying is performed only at the early flowering stage of rapeseed, the concentration of basic CuMoO4 nanosheets in the antifungal agent is 75-85 ppm; and the number of spraying is once.

[0009] According to the present invention, preferably, when spraying the rapeseed at the initial flowering stage and the pod-setting stage, the concentration of the basic CuMoO4 nanosheets in the antifungal agent is independently 35-45 ppm;

[0010] The number of spraying times during the initial flowering stage and pod-setting stage of rapeseed is once each.

[0011] According to the present invention, preferably, during the entire growth cycle of rapeseed, the basic CuMoO4 nanosheets reduce the incidence of sclerotinia sclerotiorum of rapeseed by more than 34%.

[0012] According to the present invention, preferably, the basic CuMoO4 nanosheets are prepared by a water precipitation method, which can achieve large-scale industrial preparation.

[0013] According to the present invention, preferably, the microstructure of the basic CuMoO4 nanosheet is a nanosheet structure with a thickness of 25-35 nm.

[0014] The beneficial effects of the technical solution of the present invention are as follows:

[0015] The present invention utilizes basic CuMoO4 nanosheets to prevent and treat sclerotinia sclerotiorum of rapeseed, thereby achieving increased production and income of rapeseed.

[0016] The basic CuMoO4 nanosheets of the present invention are soluble in water, and the alkalinity increases the solubility of CuMoO4 in water, so that each ion is free in water. On the one hand, the main mechanism of the basic CuMoO4 nanosheets inhibiting the sclerotinia of rapeseed sclerotinia is attributed to Cu 2+ ions, basic CuMoO4 nanosheets have the effect of enhancing the immune response of rapeseed. On the other hand, the basic CuMoO4 nanosheets have a large specific surface area, which makes Cu 2+ The contact probability between ions and fungal cells increases. At the same time, the MoO4 in the basic CuMoO4 nanosheets 2- The semiconductor physical properties increase the production of reactive oxygen species (ROS), which further enhances the antifungal effect. 2- It can open the stomata of plants, allowing them to better absorb MoO4 2- , and then obtain the trace element molybdenum at the same time.

[0017] Other features and advantages of the present invention will be described in detail in the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The above and other objects, features and advantages of the present invention will become more apparent through a more detailed description of exemplary embodiments of the present invention in conjunction with the accompanying drawings, wherein like reference numerals generally represent like components throughout the exemplary embodiments of the present invention.

[0019] Figure 1 The microstructure SEM image of the basic CuMoO4 nanosheets used in Example 1 of the present invention is shown.

[0020] FIG. 2 shows the results of a field experiment using basic CuMoO4 nanosheets to resist rapeseed sclerotinia in Example 1 of the present invention ( Figure 2AThis is the result of applying basic CuMoO4 nanosheets. Figure 2B as the control group).

[0021] Figure 3 The effect diagram of the field experiment on rapeseed using basic CuMoO4 nanosheets to resist rapeseed sclerotinia rot in Example 1 of the present invention is shown (the left side is the control group with rapeseed sclerotinia rot; the right side is the result of applying basic CuMoO4 nanosheets without rapeseed sclerotinia rot).

[0022] FIG. 4 shows the laboratory test results of the antibacterial effect of the basic CuMoO4 nanosheets of Example 1 of the present invention ( Figure 4A , d = 3.19 cm, CK is the control group; Figure 4B , d = 2.64 cm; Figure 4C , d = 1.01cm).

[0023] FIG. 5 shows the results of a mature field experiment using basic CuMoO4 nanosheets to resist rapeseed sclerotinia in Example 1 of the present invention ( Figure 5A This is the result of applying basic CuMoO4 nanosheets. Figure 5B as the control group). DETAILED DESCRIPTION

[0024] The preferred embodiments of the present invention will be described in more detail below. Although the preferred embodiments of the present invention are described below, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided to make the present invention more thorough and complete, and to fully convey the scope of the present invention to those skilled in the art.

[0025] Example 1

[0026] This embodiment provides the application of basic CuMoO4 nanosheets in the prevention and treatment of rapeseed sclerotinia disease.

[0027] The basic CuMoO4 nanosheet is prepared by a water precipitation method. The microstructure of the basic CuMoO4 nanosheet is a nanosheet structure with a thickness of 30 nm. Figure 1 shown.

[0028] This example also conducted laboratory experiments on the antibacterial effect of the basic CuMoO4 nanosheets:

[0029] The experimental methods include:

[0030] The fungal strain used was Sclerotinia sclerotiorum (CGMCC3.7083), which was purchased from the Institute of Microbiology, Chinese Academy of Sciences.

[0031] First, prepare potato dextrose agar (PDA) for fungal culture, then cool the high-temperature steam-sterilized PDA solid to 50°C and pour it into a plate on a sterile clean bench (the diameter of the circular plate culture dish is 9 cm). Then add the pre-prepared sterile basic CuMoO4 nanosheet solution (10% stock solution concentration) to the PDA solid culture medium to reach the experimental preset concentration, and make a series of gradient plates containing the basic CuMoO4 nanosheets. Use an inoculation loop to scrape the hyphae from the purchased PDA culture medium containing sclerotinia, inoculate the hyphae on a plate containing fresh PDA culture medium, and culture at 28°C for 4 days (when the hyphae cover the plate) to complete the activation. Use a sterile punch (inner diameter 5mm) to cut a 5mm diameter disc from each of the above 4-day-old PDA cultures, and inoculate the PDA disc containing sclerotia on another sterile PDA plate. Each plate is inoculated with only a PDA disc containing 5mm diameter hyphae and cultured at 28°C for 4 days to complete the second activation. And immediately used in subsequent antifungal experiments. Then, a 5 mm diameter disc was cut from each second activated culture with a sterile puncher (inner diameter 5 mm), and the 5 mm diameter PDA disc containing Sclerotinia sclerotiorum was inoculated on a PDA plate (control group) and a plate of the basic CuMoO4 nanosheet with different concentrations (experimental group). The control group and the series concentration experimental group were repeated three times. The inoculated plates were cultured in an incubator at 28°C for 36 hours. Finally, the growth radius of the mycelium of the control group and the experimental group along the inoculated agar disk was measured to calculate the inhibitory effect.

[0032] The results are shown in Figure 4. d in Figure 4 represents the diameter of the Sclerotinia disc. The smaller d is, the more inhibited the mycelial growth is. No mycelial length means that the Sclerotinia is completely killed. It can be seen that the laboratory minimum inhibitory concentration (MIC) of the basic CuMoO4 nanosheet is 100ppm. The laboratory minimum fungicidal concentration (MFC) of the basic CuMoO4 nanosheet is 1000ppm.

[0033] Field experiment on the use of basic CuMoO4 nanosheets to resist rapeseed sclerotinia:

[0034] The experimental methods include:

[0035] Zhongyouza No. 19 was used as the experimental material. The experiment was conducted at two different test sites, called Site 1 and Site 2. At each site, three different concentrations of basic nano-CuMoO4 were applied, and each concentration was repeated three times. There were nine plots in the experiment, and the area of ​​each plot was 20 square meters. The concentration of Sclerotinia sclerotiorum outdoors was lower than that in the flat plate test, so the concentration of basic nano-CuMoO4 solution required could also be reduced accordingly. The concentrations of the solution were 0ppm (clear water), 40ppm and 80ppm respectively. The solution was then sprayed on the rapeseed plants starting from the early flowering stage.

[0036] The experimental results are shown in Figures 2 and 3. Figure 2A (Location 1), Figure 3 The right side (location 2) shows the result of applying 80ppm of basic CuMoO4 nanosheets. Figure 2B (Location 1), Figure 3 Left (site 2) is the control group 0 ppm (clean water).

[0037] The embodiments of the present invention have been described above, and the above description is exemplary, not exhaustive, and is not limited to the disclosed embodiments. Many modifications and changes will be apparent to those skilled in the art without departing from the scope and spirit of the described embodiments.

Claims

1. Application of basic CuMoO4 nanosheets in the prevention and control of rapeseed sclerotinia disease.

2. The use according to claim 1, wherein: The basic CuMoO4 nanosheets are mixed with water to obtain an antifungal agent for controlling rapeseed sclerotinia disease.

3. The use according to claim 2, wherein: The control method comprises: spraying the antifungal agent at the early flowering stage of rapeseed, or spraying the antifungal agent at the early flowering stage and the pod-setting stage of rapeseed in sequence.

4. The use according to claim 3, wherein: When spraying is performed only at the early flowering stage of rapeseed, the concentration of the basic CuMoO4 nanosheets in the antifungal agent is 75-85 ppm.

5. The use according to claim 3, wherein: When spraying is only carried out during the initial flowering period of rapeseed, the spraying frequency is once.

6. The use according to claim 3, wherein: When spraying is performed at the early flowering stage and the pod-setting stage of rapeseed, the concentration of the basic CuMoO4 nanosheets in the antifungal agent is independently 35-45 ppm.

7. The use according to claim 3, wherein: When spraying is carried out in the initial flowering stage and the pod-setting stage of rapeseed successively, the spraying times in the initial flowering stage and the pod-setting stage are respectively once.

8. The use according to any one of claims 3 to 7, wherein: During the entire growth cycle of rapeseed, the basic CuMoO4 nanosheets reduced the incidence of rapeseed sclerotinia by more than 34%.

9. The use according to claim 1, wherein: The basic CuMoO4 nanosheets are prepared by a water precipitation method.

10. The use according to claim 9, wherein: The microstructure of the basic CuMoO4 nanosheet is a nanosheet structure with a thickness of 25-35 nm.

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