Equipment for detecting activity of tissue cells

Abstract

The invention discloses tissue cell activity detection equipment, and relates to the technical field of cell activity detection.The tissue cell activity detection equipment comprises a detection box, a box door is hinged to the outer wall of one side of the detection box, and a culture plate is slidably connected to the bottom of an inner cavity of the detection box; at least four sliding grooves are symmetrically formed in the inner wall of the detection box, a detection person pulls an operation handle to promote a connecting base to slide along the surface of a limiting column, a gear is meshed with a plurality of racks to promote the gear to drive a liquid storage tank to rotate by 180 degrees in a reciprocating mode, and then cell suspension is continuously shunted through a plurality of stop levers. Therefore, the liquid storage tank can be continuously rotated when the cell suspension is dripped, so that the cell suspension can be prevented from being in a static state for a long time to generate a precipitation phenomenon, the cell density distribution of the cell suspension in the holes in the culture plate can be ensured to be uniform, the accuracy and the reliability of a detection result can be improved, and the detection efficiency is improved. The quality of detection data can be improved, and meanwhile, the time and the cost of repeated detection due to inaccurate detection results are reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of cell activity detection, in particular to a tissue cell activity detection device. Background Art

[0002] Cell activity assay is an important biological technique, widely used in biology, medicine and biotechnology. It can detect cell growth, proliferation, metabolism and drug action, and provide strong support for scientific research. Common cell activity assay methods include MTT method, CCK-8 method, trypan blue staining method, etc.

[0003] At present, when detecting the activity of tissue cells by the CCK-8 method, there are usually two methods: manual dripping and automatic dripping. The automatic dripping method is to automatically drip by a special liquid dripping device, and the manual dripping method is to manually drip the cell suspension into the holes on the culture plate by the test personnel. In the process of inoculating tissue cells by manual dripping, the test personnel manually drip equal amounts of cell suspension into the holes of the culture plate in sequence. Due to the large number of holes on the culture plate, the time required for manual dripping by the test personnel is also long. Therefore, after the prepared cell suspension of tissue cells has been stationary for a long time, the tissue cells inside it may precipitate, resulting in uneven distribution of cell density in the cell suspension, which will not only affect the accuracy and reliability of the test results, but also affect the quality of the test data, thereby causing the test data to be unable to be used or referenced, and at the same time increase the time and economic cost spent on repeated tests due to inaccurate test results. Summary of the invention

[0004] In view of this, and in view of the deficiencies in the prior art, the present invention provides a tissue cell activity detection device to solve the problem in the prior art of inaccurate detection results caused by precipitation due to manual dripping of cell suspension.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a tissue cell activity detection device, comprising a detection box, a box door is hingedly connected to the outer wall of one side of the detection box, and a culture plate is slidably connected to the bottom of the inner cavity of the detection box; the inner wall of the detection box is symmetrically provided with no less than four slide grooves, and the four slide grooves are slidably connected to the inside of the sliders, and the limiting columns are fixedly connected between the opposite surfaces of the sliders, and the limiting columns are no less than two, a limiting groove is provided on one of the limiting columns, and the outer surfaces of the two limiting columns are slidably connected to a connecting seat, and a gear is rotatably connected inside the connecting seat, and the gear is located inside the limiting groove. A plurality of racks are fixedly connected at equal intervals up and down inside the limiting groove, the gear is meshed with the plurality of racks, one end of the gear shaft is fixedly connected with a liquid storage tank through a connecting seat, pipettes are symmetrically fixedly connected with the outer surfaces of both sides of the liquid storage tank, a plurality of baffles are fixedly connected with equal intervals between the relative inner walls of the liquid storage tank, two adjacent sliders are fixedly connected with operating handles through connecting grooves, and the operating handles are located outside the detection box, and the operating handles are respectively located on sliders at one end of two limiting columns; an automatic positioning mechanism is arranged on the connecting seat, a defoaming mechanism is arranged on the culture plate, and a shaking mechanism is arranged in the culture box.

[0006] Preferably, two of the four slide grooves located on opposite surfaces form a group, and the horizontal heights between the two groups are inconsistent, the horizontal heights between the two limit columns are inconsistent, and the limit groove is opened on a limit column with a lower horizontal height.

[0007] Preferably, the plurality of racks are intermittently distributed as a whole, and the plurality of racks are staggered with each other.

[0008] Preferably, the automatic positioning mechanism includes a mounting tube, which is fixedly connected to the top of the connecting seat, and a connecting slide rod is slidably connected inside the mounting tube. A limiting magnet 1 is fixedly connected to the top of the connecting slide rod, and a plurality of limiting magnets 2 are equidistantly fixedly connected to the top of the inner cavity of the detection box, the limiting magnet 1 has opposite magnetic properties to the plurality of limiting magnets 2, and the top of the limiting magnet 1 is close to the bottom of the plurality of limiting magnets 2.

[0009] Preferably, the number of the plurality of limiting magnets 2 is the same as the number of holes on the culture plate, and the plurality of limiting magnets 2 are aligned with the holes on the culture plate.

[0010] Preferably, a groove is provided in the installation tube, and ventilation holes are provided on the outer wall of the installation tube, and the ventilation holes are communicated with the groove.

[0011] Preferably, the defoaming mechanism includes a connecting column, which is fixedly connected to the outer surface of the limiting column. A mounting groove is provided on one side of the connecting column. A reciprocating slide rod is slidably connected inside the mounting groove. A telescopic spring is fixedly connected between the reciprocating slide rod and the opposite surface of the mounting groove, and the telescopic spring is located inside the mounting groove.

[0012] Preferably, a knocking ball is fixedly connected to one end of the reciprocating slide rod outside the mounting groove, and a plurality of triangular protrusions are fixedly connected to one side of the culture plate close to the connecting column at equal intervals, and the knocking ball is adapted to the triangular protrusions.

[0013] Preferably, the shaking mechanism includes a buffer groove, which is equidistantly arranged at the bottom of the inner cavity of the detection box, and a buffer magnet is symmetrically fixedly connected inside the buffer groove, and a plurality of mounting plates are equidistantly fixedly connected to the bottom of the culture plate, and the plurality of mounting plates are all located inside the buffer groove.

[0014] Preferably, buffer magnets 2 are symmetrically fixedly connected to both sides of the plurality of mounting plates, and the buffer magnets 2 have the same magnetic properties as the buffer magnet 1.

[0015] Compared with the prior art, the present invention provides a tissue cell activity detection device, which has the following beneficial effects:

[0016] The inspector manually pulls the operating handle to cause the connecting seat to slide along the surface of the lower limit column, and the gear on the connecting seat meshes with multiple racks, causing the gear to drive the liquid storage tank to reciprocate 180 degrees, and then the cell suspension is continuously diverted through multiple diamond-shaped baffles inside the liquid storage tank. Therefore, the liquid storage tank can be continuously rotated during the process of dripping the cell suspension, thereby avoiding the phenomenon of the cell suspension being in a static state for a long time and precipitating during the process of dripping the cell suspension into multiple holes on the culture plate, thereby ensuring that the cell density of the cell suspension in the multiple holes on the culture plate is evenly distributed, which can not only improve the accuracy and reliability of the test results, but also improve the quality of the test data, while also reducing the time and cost of repeated testing due to inaccurate test results.

[0017] The connecting seat drives the mounting tube to slide along the surface of the limit column, so that the limit magnet 1 on the connecting slide rod is aligned with the limit magnet 2 on the detection box, and the two are fit together with the help of magnetic attraction, so that the liquid storage tank and the hole can be automatically aligned, thereby avoiding the deviation of cell suspension dripping during manual dripping, thereby improving the operation accuracy and work efficiency, and effectively saving the time of manual operation.

[0018] The connecting seat drives the reciprocating slide rod to move synchronously, causing the knocking ball on the reciprocating slide rod to move along the inclined surfaces of the multiple triangular protrusions, and when the knocking ball is separated from the triangular protrusions, the elastic performance of the telescopic spring is used to push the knocking ball on the reciprocating slide rod to quickly hit the culture plate back and forth, thereby generating a strong impact force, causing the bubbles in the solution to be violently squeezed and stretched, thereby causing the bubble wall to rupture and release the gas therein, thereby eliminating the bubbles in the solution, thereby preventing the bubbles from interfering with the subsequent detection of the microplate reader, thereby reducing the detection error and improving the reliability and repeatability of the detection data. At the same time, it can also vibrate the cell suspension or CCK-8 solution adhered to the hole wall to promote the fusion of the cell suspension and the CCK-8 solution.

[0019] By quickly hitting the culture plate with a knocking ball, multiple mounting plates are caused to slide along the inside of the buffer groove, and the repulsive force between the buffer magnet 1 in the buffer groove and the buffer magnet 2 on the mounting plate is used to offset the impact force of the culture plate and slow down the transmission speed of the impact, thereby achieving the effect of absorbing the impact force and buffering. As a result, the cell suspension in the hole can be brought into contact with the CCK-8 solution, and the CCK-8 solution adhered to the hole wall can be shaken to the bottom of the hole cavity by continuous contact between the two. This can not only promote the mixing of the cell suspension and the CCK-8 solution, but also reduce the variability and error of the test results, and improve the reliability and accuracy of the data. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0021] Figure 2 It is a schematic diagram of the cross-section structure of the detection box in the present invention;

[0022] Figure 3 It is a schematic diagram of the structure inside the detection box of the present invention;

[0023] Figure 4 It is a schematic diagram of the cross-section structure of the liquid storage tank in the present invention;

[0024] Figure 5 It is a structural explosion diagram of the limiting cylinder and the limiting column in the present invention;

[0025] Figure 6 It is a schematic diagram of the cut-out and expanded structure of the limiting cylinder in the present invention;

[0026] Figure 7 It is an enlarged view of the structure of the second limiting magnet in the present invention;

[0027] Figure 8 It is a schematic diagram of the cross-section structure of the installation cylinder in the present invention;

[0028] Fig. 9It is an exploded view of the structure of the connecting slide rod in the present invention;

[0029] Fig.10 It is a schematic diagram of the cross-section structure of the connecting column in the present invention;

[0030] Fig.11 It is a structural exploded diagram of the reciprocating slide rod in the present invention;

[0031] Fig.12 It is a schematic diagram of the structure inside the buffer tank of the present invention;

[0032] Fig.13 It is a schematic diagram of the side structure of the buffer magnet 1 in the present invention.

[0033] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0034] 1. Testing box; 101. Box door; 102. Culture plate;

[0035] Shaking mechanism: 2, slide; 201, slider; 202, limit column; 203, limit groove; 204, connecting seat; 205, gear; 206, rack; 207, liquid storage tank; 208, pipette; 209, stop rod; 210, operating handle;

[0036] Automatic positioning mechanism: 3, mounting cylinder; 301, connecting slide rod; 302, limiting magnet 1; 303, limiting magnet 2; 304, groove; 305, vent hole;

[0037] Defoaming mechanism: 4, connecting column; 401, mounting slot; 402, reciprocating slide rod; 403, telescopic spring; 404, knocking ball; 405, triangular protrusion;

[0038] Shaking mechanism: 5, buffer groove; 501, buffer magnet one; 502, mounting plate; 503, buffer magnet two. DETAILED DESCRIPTION

[0039] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] The present invention is further described in detail below based on the accompanying drawings and embodiments.

[0041] Embodiment 1

[0042] Please refer to Figures 1 to 6As shown, in order to solve the problems mentioned in the technical solution, an embodiment of the present application provides a tissue cell activity detection device, including a detection box 1, and the detection box 1 is made of transparent material as a whole, so that the detection personnel can observe the internal conditions thereof. A box door 101 is installed on the outer wall of one side of the detection box 1, and the box door 101 is installed at the box opening of the detection box 1 in an openable and closable manner to control the opening and closing of the box opening of the detection box 1, and a culture plate 102 is slidably connected to the bottom wall of the inner cavity of the detection box 1, and a plurality of holes are equidistantly opened on the culture plate 102; two groups of slide grooves 2 are arranged on the side wall of the detection box 1, and each group of slide grooves 2 has no less than two, and the two slide grooves 2 are arranged opposite to the side wall of the detection box 1.

[0043] The height of each set of slide grooves 2 is consistent, and there is a height difference between the two sets of slide grooves 2. A slider 201 is slidably connected inside each set of slide grooves 2, and the sliders 201 arranged opposite to each other are fixedly connected to limit posts 202. In other words, the two limit posts 202 are arranged perpendicular to each other.

[0044] In addition, since there is a height difference between the two groups of slide grooves 2, there is also a height difference between the sliders 201 respectively arranged in the two groups of slide grooves 2, which makes the horizontal heights of the limit columns 202 respectively arranged between the two relatively arranged sliders 201 inconsistent, and the limit groove 203 is opened on the limit column 202 with a lower horizontal height.

[0045] The outer surfaces of the two limit posts 202 are slidably connected to a connection seat 204. More specifically, a channel is provided at the bottom end of the connection seat 204, and the channel allows the limit posts 202 with a lower level to pass through. A gear 205 is rotatably connected inside the connection seat 204, and the gear 205 is installed in the channel.

[0046] The top wall and the bottom wall of the limiting groove 203 are fixedly connected with multiple groups of racks 206 at equal intervals, and the number of teeth of the group of racks 206 located at the outermost side of the limiting groove 203 is less than the number of teeth of the remaining groups of racks 206. The group of racks 206 with fewer teeth is the starting end of the gear 205 meshing with it. More specifically, the group of racks 206 with fewer teeth is half of the number of teeth of the remaining groups of racks 206, so the rotation angle of the gear 205 on the group of racks 206 with fewer teeth is half of the rotation angle on the remaining groups of racks 206, thereby making the liquid storage tank 207 before it moves to the top of the initial hole, and the two pipettes 208 on it are in a vertical distribution. The multiple groups of racks 206 arranged on the top wall and the bottom wall are all arranged at intervals, and the racks 206 arranged on the top wall and the bottom wall are arranged in an interlaced manner. The gear 205 meshes with the racks 206.

[0047] The gear 205 is rotatably connected to the channel via a rotating shaft, one end of the rotating shaft passes through the connecting seat 204 and is fixedly connected to a liquid storage tank 207, and the liquid storage tank 207 is provided with an openable and closable tank cover, so that the inspector can inject the cell suspension by opening the tank cover.

[0048] Preferably, the rotating shaft is installed in the middle of the liquid storage tank 207 to ensure that the two ends of the liquid storage tank 207 are evenly stressed and more stable when installed. In addition, half of the length of the liquid storage tank 207 is less than the height difference between the two limit posts 202, thereby preventing the liquid storage tank 207 from colliding with the limit posts 202 during rotation.

[0049] The outer surfaces of both sides of the liquid storage tank 207 are symmetrically fixedly connected with pipettes 208, and the positions of the parallel projections of the multiple sets of racks 206 on the culture plate 102 are aligned with the gaps between the multiple holes on the culture plate 102, so the multiple sets of racks 206 arranged on the top wall and the bottom wall are relatively staggered, thereby preventing the liquid storage tank 207 from rotating when the cell suspension is dripped into the holes through the pipette 208, resulting in the cell suspension not being accurately dripped into the holes. Multiple baffles 209 are equidistantly fixedly connected between the relative inner walls of the liquid storage tank 207, wherein the cross-sections of the multiple baffles 209 are all rhombus-shaped, and the surfaces of the multiple baffles 209 are not parallel to the upper and lower surfaces of the liquid storage tank 207, thereby avoiding a small amount of cell suspension remaining on the multiple baffles 209. Two adjacent sliders 201 are fixedly connected with operating handles 210 through connecting grooves, and the operating ends of the operating handles 210 are located outside the detection box 1 , and one of the operating handles 210 is located at one side of the box door 101 .

[0050] Working process: Before use, the liquid storage tank 207 is in a horizontal state, and the two pipettes 208 are also in a horizontal state, and the liquid storage tank 207 together with the two limiting columns 202 are close to a corner inside the detection box 1.

[0051] When in use, the inspector first opens the box door 101, then opens the tank cover on the liquid storage tank 207, and injects the prepared cell suspension into the liquid storage tank 207 through the feed port sealed by the tank cover. Then the inspector manually pushes the operating handle 210 located at a higher position, so that the two sliders 201 drive the limiting columns 202 thereon to slide synchronously along the inside of the slide groove 2, and the connecting seat 204 thereon slides along the outer surface of the limiting column 202 at the lower position, and causes the liquid storage tank 207 on the connecting seat 204 to perform horizontal lateral movement. During this process, the gear 205 on the connecting seat 204 first meshes with the rack 206 set on the bottom wall of the limiting groove 203, so that the gear 205 rotates ninety degrees. At this time, the pipette 208 on the liquid storage tank 207 is moved horizontally. The state is converted into a vertical state, thereby the cell suspension can be dripped into the holes on the culture plate 102, and as the connecting seat 204 continues to slide, the gear 205 meshes with the rack 206 on the upper part of the limiting groove 203. At this time, the rotation direction of the gear 205 is opposite to that before, and the gear 205 rotates 180 degrees accordingly, causing the two pipettes 208 on the liquid storage tank 207 to switch positions, and the cell suspension in the liquid storage tank 207 also flows from one side to the other side. During the flow of the cell suspension, it continuously contacts the multiple diamond-shaped baffles 209 inside the liquid storage tank 207, and is continuously diverted by the two sides of the multiple baffles 209 that are not parallel to the liquid storage tank 207, thereby promoting the mixing effect between the tissue cells and the culture fluid. When the work of dripping the cell suspension into a hole is completed, the gear 205 is meshed with the rack 206 at the bottom of the limiting groove 203 again. Similarly, the gear 205 drives the liquid storage tank 207 to rotate 180 degrees again, and the two pipettes 208 on the liquid storage tank 207 are switched again. This cycle is repeated over and over again. The liquid storage tank 207 can be continuously rotated during the process of dripping the cell suspension. This can avoid the phenomenon that the cell suspension is in a static state for a long time and precipitates during the process of dripping the cell suspension into multiple holes on the culture plate 102. There is no need for an additional shaking step, and the operation is more convenient. This can ensure that the cell density of the cell suspension in the multiple holes on the culture plate 102 is evenly distributed, which can not only improve the accuracy and reliability of the test results, but also improve the quality of the test data, while also reducing the time and cost of repeated tests due to inaccurate test results.

[0052] Embodiment 2

[0053] Please refer to Figures 7 to 9As shown, an automatic positioning mechanism is provided on the connection seat 204, and the automatic positioning mechanism includes a mounting tube 3, which is fixedly connected to the top of the connection seat 204, and a connecting slide bar 301 is slidably connected inside the mounting tube 3, wherein the connecting slide bar 301 is made of a lightweight material to ensure that the connecting slide bar 301 can be pulled by the limiting magnet 1 302, and the central axis of the connecting slide bar 301 coincides with the central axis of the two pipettes 208 in a vertical state, so as to ensure that the two pipettes 208 are aligned with the holes on the culture plate 102. The top of the connecting slide bar 301 is fixedly connected to the limiting magnet 1 302, and the top wall of the inner cavity of the detection box 1 is fixedly connected to multiple limiting magnets 2 303 at equal distances, and the magnetic properties of the limiting magnet 1 302 and the limiting magnet 2 303 are oppositely arranged, and the top of the limiting magnet 1 302 is close to the bottom of the multiple limiting magnets 2 303. The number of the plurality of second limiting magnets 303 is the same as the number of holes on the culture plate 102, and the plurality of second limiting magnets 303 are aligned with the holes on the culture plate 102. A groove 304 is provided in the installation tube 3, and at least one vent hole 305 is provided on the outer wall of the installation tube 3, and the vent holes 305 are all connected to the groove 304.

[0054] Working process: when the connection seat 204 drives the liquid storage tank 207 to slide along the outer surface of the limiting column 202, the connection seat 204 drives the installation tube 3 to move synchronously. When the gear 205 rotates 180 degrees along the rack 206, the limiting magnet 1 302 on the connecting slide bar 301 is aligned with the limiting magnet 2 303 on the detection box 1, and under the action of the mutual attraction generated between the two, the connecting slide bar 301 slides upward along the inside of the installation tube 3. In this process, the internal space of the installation tube 3 at the lower part of the connecting slide bar 301 gradually increases, and its internal air pressure gradually decreases, so that the pressure difference between the inside and outside of the installation tube 3 increases. At the same time, due to the lower air pressure of the installation tube 3, the external air flows into the groove 304 from the vent hole 305 The air pressure inside the mounting tube 3 is made equal to the external atmospheric pressure to ensure that the connecting slide bar 301 slides upward along the inside of the mounting tube 3, and the limiting magnet 1 302 moves upward rapidly due to the attraction and fits with the limiting magnet 2 303, and provides a certain resistance to the sliding of the connecting seat 204, so that a sense of frustration can be formed when the liquid storage tank 207 moves to above the hole on the culture plate 102, and the sense of frustration is transmitted to the hands of the inspector through the limiting column 202 and the operating handle 210, thereby allowing the inspector to promptly realize that the pipette 208 on the liquid storage tank 207 is aligned with the hole on the culture plate 102 and stop pushing the operating handle 210, and the position of the pipette 208 can be observed in conjunction with the transparent material of the detection box 1. At this time, the pipette 208 located at a lower position on the liquid storage tank 207 is close to the culture plate 102, and then the quantitative cell suspension is automatically squeezed out by the pipette 208 and falls into the hole aligned with it under the action of gravity. After the dripping is completed, the inspector continues to push the operating handle 210, so that the connecting seat 204 continues to slide along the outer surface of the limiting column 202. At this time, because the pushing force of the inspector is greater than the attraction between the limiting magnet 1 302 and the limiting magnet 2 303, the two are separated from each other, and the connecting slide rod 301 slides downward along the inside of the installation tube 3 due to the action of gravity. In this process, the connecting slide rod 301 As the internal space of the lower installation tube 3 gradually decreases, its internal air pressure gradually increases, making its internal air pressure greater than the external atmospheric pressure. As a result, the high-pressure air inside is discharged from the outside of the installation tube 3 through the vent hole 305 on the groove 304 by the extrusion of the gravity of the limiting magnet 1 302, and then the connecting slide bar 301 can be squeezed to return to its original position. Similarly, the limiting magnet 1 302 and the limiting magnet 2 303 fit each other, so that the liquid storage tank 207 is automatically aligned with the hole, thereby avoiding the situation of cell suspension dripping deviation during manual dripping, thereby improving the operation accuracy and labor efficiency, and effectively saving the time of manual operation. After the dripping of the holes in a horizontal row of the culture plate 102 is completed, the inspection personnel then manually push the operating handle 210 located on the lower limiting column 202, so that the connection seat 204 moves horizontally and vertically, thereby dripping the next horizontal row of holes.

[0055] Embodiment 3

[0056] Please refer to Fig.10 as well as Fig.11 As shown, a defoaming mechanism is provided on the culture plate 102, and the defoaming mechanism includes a connecting column 4, which is fixedly connected to the outer surface of the limiting column 202 located at a lower position, wherein the connecting column 4 is L-shaped as a whole, and its lower position is located at the same horizontal plane as the middle of the side of the culture plate 102. A mounting groove 401 is provided on one side of the connecting column 4, and a reciprocating slide 402 is slidably connected inside the mounting groove 401, and the length of the reciprocating slide 402 is greater than the distance between the connecting column 4 and the culture plate 102. A telescopic spring 403 is fixedly connected inside the mounting groove 401, and the end of the reciprocating slide 402 away from the culture plate 102 contacts the end of the telescopic spring 403 close to the reciprocating slide 402. A knocking ball 404 is fixedly connected to one end of the reciprocating slide rod 402 outside the mounting groove 401, and a plurality of triangular protrusions 405 are fixedly connected to one side of the culture plate 102 close to the connecting column 4 at equal intervals. Both sides of the triangular protrusion 405 are set as inclined surfaces, and the knocking ball 404 is adapted to the triangular protrusion 405.

[0057] Initial state: the telescopic spring 403 is in a normal state without being affected by external force, and the knocking ball 404 is in contact with one side of the culture plate 102 .

[0058] Working process: when the connecting seat 204 moves horizontally and longitudinally, the connecting column 4 on the lower limiting column 202 also moves horizontally and vertically, and then the reciprocating slide bar 402 on the connecting column 4 drives the knocking ball 404 to move synchronously with it. During this process, the knocking ball 404 moves along the surface of the triangular protrusion 405, and is squeezed by its inclined surface to push the reciprocating slide bar 402 to slide inside the installation groove 401, so that the telescopic spring 403 inside the installation groove 401 is forced to shrink. When the knocking ball 404 moves to the highest point of the inclined surface of the triangular protrusion 405 and disengages from it, the telescopic spring 403 loses its restriction and returns to its original state. The telescopic spring 403 pushes the reciprocating slide bar 402 to slide outside the installation groove 401, and drives the knocking ball 404 on the reciprocating slide bar 402 to the culture plate 102 One side of the well 102 moves quickly, so that the knocking ball 404 hits the culture plate 102, causing the culture plate 102 to vibrate slightly due to the impact force, thereby promoting some bubbles generated by the dropwise addition of the cell suspension or the subsequent addition of the CCK-8 solution to move to the surface of the solution and eventually escape, and the energy generated by the vibration acts on some bubbles and causes them to deform, eventually burst and release the gas therein, thereby preventing the bubbles from interfering with the subsequent detection of the microplate reader, thereby reducing the detection error and improving the reliability and repeatability of the detection data, and at the same time, vibrating the cell suspension adhered to the hole wall or the subsequent addition of the CCK-8 solution with greater viscosity, causing the two to flow downward along the hole wall due to the vibration force and promoting the fusion of the cell suspension and the CCK-8 solution.

[0059] Embodiment 4

[0060] Please refer to Figures 10 to 13As shown, a shaking mechanism is provided in the detection box 1, and the shaking mechanism includes a buffer groove 5, which is equidistantly arranged at the bottom of the inner cavity of the detection box 1, and a buffer magnet 1 501 is symmetrically fixedly connected inside the buffer groove 5, and a plurality of mounting plates 502 are equidistantly fixedly connected to the bottom of the culture plate 102, and the plurality of mounting plates 502 are all located inside the buffer groove 5. Buffer magnets 2 503 are symmetrically fixedly connected on both sides of the plurality of mounting plates 502, and the magnetic properties of the buffer magnets 2 503 and the buffer magnets 1 501 are the same, wherein the force generated by the knocking ball 404 on the reciprocating slide 402 knocking the side of the culture plate 102 is greater than the repulsive force between the buffer magnets 1 501 and the buffer magnets 2 503, thereby ensuring that the culture plate 102 is vibrated due to multiple knockings, and at the same time, it can also avoid the situation where the cells in the cell suspension are damaged due to excessive knocking force. The multiple mounting plates 502 are all located in the middle of the buffer slot 5 to ensure that the spacing between the buffer magnet 2 503 and the buffer magnet 1 501 on both sides of the multiple mounting plates 502 is equal, thereby making the displacement distance of the horizontal lateral shaking of the culture plate 102 equal, and further promoting the cell suspension in the multiple holes on the culture plate 102 to have a consistent reciprocating shaking amplitude, avoiding excessive shaking amplitude that causes the cell suspension to flow from the holes to the gaps between the holes.

[0061] Working process: When the knocking ball 404 hits the culture plate 102 quickly, the multiple mounting plates 502 at the bottom of the culture plate 102 slide along the inside of the buffer groove 5 to the side away from the knocking ball 404, causing the buffer magnets 2 503 on the multiple mounting plates 502 to quickly approach the buffer magnet 1 501, so that a repulsive force is generated between the two, and a gap is left between the two, and then the impact force generated by the impact of the knocking ball 404 is gradually offset by the continuous repulsive force between the buffer magnet 2 503 and the buffer magnet 1 501, and then when the impact force is eliminated, the repulsive force between the buffer magnet 2 503 and the buffer magnet 1 501 pushes the multiple mounting plates 502 to slide in the opposite direction along the buffer groove 5 again, so that the multiple mounting plates 502 The buffer magnet 2 503 on the other side is close to the buffer magnet 1 501 on the other side of the buffer slot 5. Similarly, it can absorb part of the impact force on the culture plate 102 and slow down the transmission speed of the impact, thereby achieving the effect of absorbing impact force and buffering. During this process, the cell suspension in the holes on the culture plate 102 can be slightly shaken left and right inside, so that the cell suspension in the holes can contact with the CCK-8 solution with higher viscosity on the hole wall, and then the CCK-8 solution adhering to the hole wall can be cleaned to the bottom of the hole by continuous contact between the two. This can not only promote the mixing of the cell suspension and the CCK-8 solution, but also reduce the variability and error of the test results, and improve the reliability and accuracy of the data.

[0062] The above is a detailed introduction to the present application. Specific examples are used in this article to illustrate the principles and implementation methods of the present application. The description of the above embodiments is only used to help understand the method of the present application and its core idea. At the same time, for general technical personnel in this field, according to the idea of ​​the present application, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as a limitation on the present application.

Claims

1. A tissue cell activity detection device, characterized in that: It comprises a detection box (1), wherein a box door (101) is installed on an outer wall of one side of the detection box (1), a culture plate (102) is slidably connected to the bottom wall of the inner cavity of the detection box (1), and a plurality of holes are opened on the culture plate (102) at equal intervals; Two groups of slide grooves (2) are arranged on the side wall of the detection box (1), each group of the slide grooves (2) has no less than two, the two slide grooves (2) are arranged opposite to each other on the side wall of the detection box (1), each group of the slide grooves (2) is slidably connected with a slider (201), the sliders (201) arranged opposite to each other are fixedly connected with a limiting column (202), and there are no less than two limiting columns (202), one of the limiting columns (202) is provided with a limiting groove (203), the outer surfaces of the two limiting columns (202) are slidably connected with a connecting seat (204), the bottom end of the connecting seat (204) is provided with a channel, the connecting seat (204) is rotatably connected with a gear (205) inside, the gear (205) is installed in the channel, the limiting groove (203) is provided on the connecting seat (204), and the connecting seat (204) is provided with a gear (205) installed in the channel. The top wall and the bottom wall of the detection box (203) are fixedly connected with a plurality of racks (206) at equal intervals, the gear (205) and the rack (206) are meshed with each other, the gear (205) is rotatably connected to the channel via a rotating shaft, and one end of the rotating shaft passes through the connecting seat (204) and is fixedly connected with a liquid storage tank (207), the outer surfaces of both sides of the liquid storage tank (207) are symmetrically fixedly connected with a pipette (208), and a plurality of blocking rods (209) are fixedly connected with equal intervals between the relative inner walls of the liquid storage tank (207), wherein two adjacent sliders (201) are fixedly connected with operating handles (210) via connecting grooves, and the operating ends of the operating handles (210) are located outside the detection box (1), and one of the operating handles (210) is located on one side of the box door (101); An automatic positioning mechanism is arranged on the connection seat (204), the automatic positioning mechanism comprising a mounting tube (3), the mounting tube (3) being fixedly connected to the top of the connection seat (204), a connecting slide rod (301) being slidably connected inside the mounting tube (3), a limiting magnet one (302) being fixedly connected to the top of the connecting slide rod (301), a plurality of limiting magnets two (303) being fixedly connected to the top wall of the inner cavity of the detection box (1) at equal intervals, the magnetic properties of the limiting magnet one (302) and the plurality of limiting magnets two (303) being arranged oppositely, and the top of the limiting magnet one (302) is close to the bottom of the plurality of limiting magnets two (303).

2. The tissue cell activity detection device according to claim 1, characterized in that: The height of each group of the slide grooves (2) is consistent, there is a height difference between the two groups of the slide grooves (2), the two limit columns (202) are arranged perpendicular to each other, and the horizontal heights of the limit columns (202) between the two oppositely arranged sliders (201) are inconsistent.

3. The tissue cell activity detection device according to claim 2, characterized in that: The plurality of groups of racks (206) are distributed at intervals as a whole, and the plurality of groups of racks (206) are arranged in an interlaced manner.

4. The tissue cell activity detection device according to claim 3, characterized in that: The number of the plurality of limiting magnets 2 (303) is the same as the number of holes on the culture plate (102), and the plurality of limiting magnets 2 (303) are aligned with the holes on the culture plate (102).

5. The tissue cell activity detection device according to claim 4, characterized in that: A groove (304) is provided in the installation tube (3), and at least one vent hole (305) is provided on the outer wall of the installation tube (3), and the vent holes (305) are all connected to the groove (304).

6. The tissue cell activity detection device according to claim 5, characterized in that: A defoaming mechanism is provided on the culture plate (102), and the defoaming mechanism comprises a connecting column (4), wherein the connecting column (4) is fixedly connected to the outer surface of a limiting column (202) located at a lower position, and a mounting groove (401) is provided on one side of the connecting column (4), and a reciprocating slide rod (402) is slidably connected inside the mounting groove (401), and a telescopic spring (403) is fixedly connected inside the mounting groove (401), and an end of the reciprocating slide rod (402) away from the culture plate (102) contacts an end of the telescopic spring (403) close to the reciprocating slide rod (402).

7. The tissue cell activity detection device according to claim 6, characterized in that: A knocking ball (404) is fixedly connected to one end of the reciprocating slide rod (402) located outside the mounting groove (401), and a plurality of triangular protrusions (405) are fixedly connected at equal intervals to one side of the culture plate (102) close to the connecting column (4), and both sides of the triangular protrusions (405) are arranged as inclined surfaces, and the knocking ball (404) is adapted to fit the triangular protrusions (405).

8. The tissue cell activity detection device according to claim 1, characterized in that: A shaking mechanism is arranged in the detection box (1), and the shaking mechanism comprises a buffer groove (5), the buffer groove (5) is equidistantly arranged at the bottom of the inner cavity of the detection box (1), a buffer magnet (501) is symmetrically fixedly connected inside the buffer groove (5), and a plurality of mounting plates (502) are equidistantly fixedly connected to the bottom of the culture plate (102), and the plurality of mounting plates (502) are all located inside the buffer groove (5).

9. The tissue cell activity detection device according to claim 8, characterized in that: The two sides of the plurality of mounting plates (502) are symmetrically fixedly connected with buffer magnets 2 (503), and the magnetic properties of the buffer magnets 2 (503) are the same as those of the buffer magnets 1 (501).

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