All-natural plant extract compound feed additive
By adopting the composite feed additives of all -natural plant extracts, the problem of limited side effects and efficacy of existing feed additives is solved, and the effect of improving animal immunity and disease resistance, reducing antibiotics, and improving the safety of meat products.
Patent Information
- Application Number
- CN202411333980.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-24
- Publication Date
- 2025-01-28
AI Technical Summary
Existing feed additives have side effects, such as drug resistance, drug residues and hyperpancreatic function, and the limited efficacy of a single plant extract makes it difficult to improve animal health in an overall manner.
Compound feed additives with all natural plant extracts, including various plant extracts such as Eucommia leaves, cymbals, rough old tea leaves, ramie roots, mushroom roots, purslane, wild chrysanthemum, custody, polysaccharide, Achyranton polysaccharide, bonito, polysaccharide, Achyroxa, Purple, yeast cell walls, deodorbia jelviated dry bacteria, corn powder, bran powder, protein mulberry plant powder and egg butter, which improves the immunity and disease resistance of animals through scientific reinforcement.
Effectively improve the immunity and disease resistance of animals, reduce the number of antibiotics, increase the safety of animal meat products, enhance the resistance of animals, improve the benefits of breeding, and avoid damage caused by compounds.
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Abstract
Description
Technical Field
[0001] The invention relates to the technical field of feed additive preparation, in particular to a fully natural plant extract composite feed additive. Background Art
[0002] Animal husbandry is an important industry in my country's agriculture and plays an important role in ensuring the safe supply of meat food. At present, my country's animal husbandry is changing from traditional animal husbandry to modern animal husbandry, and significant changes are taking place in animal husbandry mode, regional layout, production method and production capacity. Existing feed additives are mainly chemical drug additives, and some Chinese herbal medicine additives and a small amount of active biological additives are also used.
[0003] Traditionally, widely used chemical drug additives include antibiotics, hormones, chemical synthetic drugs, etc., which are commonly used to prevent and treat livestock, poultry, and aquatic animal diseases and as growth promoters. The disadvantage of this type of additive is that long-term use has great side effects. On the one hand, it can cause pathogenic microorganisms to develop drug resistance, reducing the prevention and treatment effect of animal diseases; on the other hand, it can produce drug residues in meat, eggs, milk and the environment, which seriously threatens human health. For this reason, many countries in the world have restricted the use of chemical drug additives and gradually eliminated or banned them. Active biological additives mainly refer to microbial active preparations made from some active bacteria that are beneficial to animals. Its disadvantage is that the cost is relatively high, and when it is stored in the external environment for a long time, its survival rate will decrease, affecting the use effect.
[0004] A plant extract compound feed additive provided by the publication number "CN115997847A" is made of the following raw materials in parts by weight: 20-26 parts of fish meal, 12-16 parts of peanut meal, 8-11 parts of soybean meal, 3-5 parts of soybean oil, 2.5-3.5 parts of brewer's yeast, 1.5-2.5 parts of choline chloride, 4-6 parts of soybean lecithin, 3-5 parts of calcium dihydrogen phosphate, and 3-5 parts of compound plant extract. The plant extract compound feed additive of the present invention can improve the immunity of farmed animals, thereby avoiding problems such as drug residues caused by the use of antibiotics; it can improve the antioxidant capacity of farmed animals, thereby avoiding the toxic and side effects caused by the addition of chemically synthesized antioxidants; in addition, it also has obvious antibacterial and antiviral and growth-promoting effects, and is better than the commonly used feed antibiotics chlortetracycline or roxithromycin in preventing diseases and gaining weight; it has anti-heat stress effect, good palatability, enhances appetite, and can improve the utilization rate of feed.
[0005] However, the above device still has the following problems during implementation:
[0006] 1. Using a single plant extract as a feed additive, the product has a single efficacy, and the types and contents of various active ingredients are limited, making it difficult to improve the overall health of animals and fundamentally improve the disease prevention and resistance of animals;
[0007] 2. Adding a large amount of compound ingredients will cause the animal's growth to slow down or stagnate, causing pancreatic hypertrophy and hyperfunction of the pancreas, leading to excessive pancreatic secretion and causing endogenous loss of essential amino acids (especially sulfur-containing amino acids). Summary of the invention
[0008] The object of the present invention is to provide a fully natural plant extract compound feed additive to solve the problems raised in the above background technology.
[0009] To achieve the above object, the present invention provides the following technical solutions:
[0010] The all-natural plant extract compound feed additive comprises the following plant extracts in parts by weight: 3-12 parts of eucommia leaf extract, 5-12 parts of alfalfa extract, 8-10 parts of crude old tea extract, 3-10 parts of ramie root and leaf extract, 6-10 parts of mushroom root extract, 2-9 parts of purslane extract, 1-8 parts of wild chrysanthemum extract, 5-8 parts of tangerine peel extract, 6-8 parts of Centella asiatica extract, 12-26 parts of Achyranthes bidentata polysaccharide, 15-60 parts of Perilla frutescens extract, 35-55 parts of yeast cell wall extract, 8-16 parts of freeze-dried Bacillus subtilis powder, 35-60 parts of corn flour, 45-60 parts of bran powder, 70-85 parts of protein mulberry plant powder and 15-40 parts of egg yolk oil.
[0011] Preferably, the plant extracts include the following parts by weight: 70 parts of protein mulberry plant powder, 35 parts of corn flour, 45 parts of bran powder, 8 parts of crude old tea extract, 3 parts of eucommia leaf extract, 5 parts of alfalfa extract, 30 parts of ramie root and leaf extract, 6 parts of mushroom root extract, 2 parts of purslane extract, 1 part of wild chrysanthemum extract, 5 parts of tangerine peel extract, 6 parts of Centella asiatica extract, 12 parts of Achyranthes bidentata polysaccharide, 15 parts of Perilla extract, 35 parts of yeast cell wall extract, 8 parts of freeze-dried Bacillus subtilis powder, and 15 parts of egg yolk oil.
[0012] Preferably, the plant extracts include the following parts by weight: 12 parts of eucommia leaf extract, 12 parts of alfalfa extract, 10 parts of crude old tea extract, 10 parts of ramie root and leaf extract, 10 parts of mushroom root extract, 9 parts of purslane extract, 8 parts of wild chrysanthemum extract, 8 parts of tangerine peel extract, 8 parts of Centella asiatica extract, 26 parts of Achyranthes bidentata polysaccharide, 60 parts of Perilla frutescens extract, 55 parts of yeast cell wall extract, 16 parts of freeze-dried Bacillus subtilis powder, 60 parts of corn flour, 60 parts of bran powder, 85 parts of protein mulberry plant powder, and 40 parts of egg yolk oil.
[0013] Preferably, the plant extracts include the following parts by weight: 10 parts of eucommia leaf extract, 8 parts of alfalfa extract, 9 parts of crude old tea extract, 6 parts of ramie root and leaf extract, 8 parts of mushroom root extract, 5 parts of purslane extract, 7 parts of wild chrysanthemum extract, 6 parts of tangerine peel extract, 7 parts of Centella asiatica extract, 20 parts of Achyranthes bidentata polysaccharide, 35 parts of Perilla frutescens extract, 45 parts of yeast cell wall extract, 12 parts of freeze-dried Bacillus subtilis powder, 45 parts of corn flour, 50 parts of bran powder, 78 parts of protein mulberry plant powder, and 30 parts of egg yolk oil.
[0014] Preferably, the preparation method of the ramie root and leaf extract includes: the ramie root and leaves are crushed and passed through a 30-50 mesh sieve, then the Centella asiatica extract soaking solution is added and heated, the temperature is controlled at 45-60°C, and after 1.5-3 hours, the extract is concentrated and dried to obtain a ramie root and leaf extract, the extract is vacuum concentrated and then spray-dried to obtain a ramie root and leaf extract, and the ramie root and leaf extract is crushed and sieved to obtain the ramie root and leaf extract.
[0015] Preferably, the preparation method of the eucommia leaf extract includes: the eucommia leaves are crushed and passed through a 30-60 mesh sieve, hot water of 2-5 times the weight of the eucommia leaves is added, the extract is collected 2-3 times at intervals, each time for 30-50 minutes, the temperature of the hot water is 70-85°C, ethanol is added to the extract so that the ethanol concentration in the extract reaches 70-85%, and the concentration is maintained for 2-3 hours, the precipitate is collected, dried at 60-75°C, crushed, and passed through a 40 mesh sieve to obtain the eucommia leaf extract.
[0016] Preferably, the preparation method of the Achyranthes bidentata polysaccharide includes: crushing the Achyranthes bidentata and passing it through a 40-50 mesh sieve, adding 70-85°C hot water in an amount 2-4 times the weight of the Achyranthes bidentata and continuously extracting for 2-3 times, each time for 30-50 minutes; collecting the extract, adding ethanol to the extract so that the ethanol concentration in the extract reaches 70-85%, maintaining for 2-3 hours, collecting the precipitate, drying at 60-75°C, and then crushing it and passing it through a 20-30 mesh sieve to obtain Achyranthes bidentata polysaccharide.
[0017] Preferably, the preparation method of the Achyranthes bidentata polysaccharide includes: removing impurities from the protein mulberry, grinding it into powder, adding water with a volume 8 times the volume of the protein mulberry powder, refluxing for 2.5 hours, filtering and collecting the filtrate, adding water with a volume 5 times the volume of the white mulberry powder to the filter residue again, and continuing to reflux for 2 hours; filtering and collecting the filtrate, combining the two filtrates, concentrating and evaporating to dryness.
[0018] Preferably, the preparation method of the freeze-dried Bacillus subtilis powder includes: uniformly dispersing the Bacillus subtilis suspension in a freeze-drying protective agent solution to form a Bacillus subtilis suspension, dispensing the suspension into vials, precooling the suspension in an ice water bath, prefreezing the suspension in liquid nitrogen, transferring the precooled and prefrozen Bacillus subtilis suspension to a freeze dryer, and performing vacuum drying to obtain the Bacillus subtilis freeze-dried powder.
[0019] Compared with the prior art, the present invention has the following beneficial effects:
[0020] 1. The components provided by the present invention are added with purslane, which has the functions of clearing away heat and detoxifying, promoting diuresis and removing dampness, dispersing blood and reducing swelling, anti-inflammatory, sterilizing, analgesic and hemostatic, and anti-allergic; it has the physiological functions of mild laxative, healing, anti-inflammatory, expectorant and purification, and is combined with the added Centella asiatica extract, Achyranthes bidentata polysaccharide, Perilla frutescens extract, yeast cell wall extract, Bacillus subtilis freeze-dried powder, and egg yolk oil, which can resist common pathogens and reduce allergic reactions, thereby controlling diarrhea caused by bacteria, fungi and nutritional antigens and enhancing immune function.
[0021] 2. The present invention adopts a whole-plant extract compound feed additive, which can not only avoid the harm caused by compounds to animals, but also utilizes the scientific compound of Achyranthes bidentata polysaccharide and yeast cell wall extract, Eucommia ulmoides leaf extract, alfalfa extract, coarse old tea extract, ramie root and leaf extract and mushroom root extract to effectively reduce the amount of antibiotics used in feeding animals, improve the safety of animal meat products, enhance the resistance of animals, and improve feeding efficiency. DETAILED DESCRIPTION
[0022] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.
[0023] The invention provides a technical scheme: a fully natural plant extract compound feed additive, comprising the following plant extracts in parts by weight: 35-55 parts of yeast cell wall extract, 3-12 parts of eucommia leaf extract, 5-12 parts of alfalfa extract, 8-10 parts of crude old tea extract, 3-10 parts of ramie root and leaf extract, 6-10 parts of mushroom root extract, 2-9 parts of purslane extract, 1-8 parts of wild chrysanthemum extract, 5-8 parts of tangerine peel extract, 6-8 parts of Centella asiatica extract, 12-26 parts of Achyranthes bidentata polysaccharide, 15-60 parts of Perilla extract, 8-16 parts of freeze-dried bacillus subtilis powder, 35-60 parts of corn flour, 45-60 parts of bran powder, 70-85 parts of protein mulberry plant powder and 15-40 parts of egg yolk oil.
[0024] Embodiment 1:
[0025] The all-natural plant extract compound feed additive comprises the following plant extracts in parts by weight: 70 parts of protein mulberry plant powder, 35 parts of corn flour, 45 parts of bran powder, 8 parts of crude old tea extract, 3 parts of eucommia leaf extract, 5 parts of alfalfa extract, 30 parts of ramie root and leaf extract, 6 parts of mushroom root extract, 2 parts of purslane extract, 1 part of wild chrysanthemum extract, 5 parts of tangerine peel extract, 6 parts of Centella asiatica extract, 12 parts of Achyranthes bidentata polysaccharide, 15 parts of Perilla frutescens extract, 35 parts of yeast cell wall extract, 8 parts of freeze-dried Bacillus subtilis powder and 15 parts of egg yolk oil.
[0026] In this embodiment, the preparation method of the ramie root and leaf extract is included: the ramie root and leaves are crushed and passed through a 30-50 mesh sieve, and then a Centella asiatica extract soaking solution is added and heated, and the temperature is controlled at 45-60°C. After 1.5-3 hours, the extract is concentrated and dried to obtain a ramie root and leaf extract, and the extract is vacuum concentrated and spray-dried to obtain a ramie root and leaf extract, which is crushed and sieved to obtain a ramie root and leaf extract.
[0027] In this embodiment, the preparation method of the Eucommia leaf extract is as follows: the Eucommia leaves are crushed and passed through a 30-60 mesh sieve, hot water of 2-5 times the weight of the Eucommia leaves is added, and the extract is collected 2-3 times at intervals, each time for 30-50 minutes, the hot water temperature is 70-85°C, ethanol is added to the extract so that the ethanol concentration in the extract reaches 70-85%, and maintained for 2-3 hours, the precipitate is collected, dried at 60-75°C, crushed, and passed through a 40 mesh sieve to obtain the Eucommia leaf extract.
[0028] In this embodiment, the preparation method of the Achyranthes bidentata polysaccharide is included: the Achyranthes bidentata is crushed and passed through a 40-50 mesh sieve, and 70-85°C hot water in an amount 2-4 times the weight of the Achyranthes bidentata is added and extracted continuously for 2-3 times, each time for 30-50 minutes; the extract is collected, and ethanol is added to the extract so that the ethanol concentration in the extract reaches 70-85%, and maintained for 2-3 hours, and the precipitate is collected, dried at 60-75°C, and then crushed and passed through a 20-30 mesh sieve to obtain Achyranthes bidentata polysaccharide.
[0029] In this embodiment, the preparation method of the Achyranthes bidentata polysaccharide is as follows: removing impurities from protein mulberry, grinding it into powder, adding water with a volume 8 times the volume of the protein mulberry powder, refluxing for 2.5 hours, filtering and collecting the filtrate, adding water with a volume 5 times the volume of the white mulberry powder to the filter residue again, and continuing to reflux for 2 hours; filtering and collecting the filtrate, combining the two filtrates, concentrating and evaporating to dryness.
[0030] In this embodiment, the preparation method of the Bacillus subtilis freeze-dried powder includes: uniformly dispersing the Bacillus subtilis suspension in a freeze-drying protective agent solution to form a Bacillus subtilis suspension, packaging the suspension in vials, precooling the suspension in an ice water bath, and prefreezing the suspension in liquid nitrogen. The precooled and prefrozen Bacillus subtilis suspension is transferred to a freeze dryer and vacuum dried to obtain Bacillus subtilis freeze-dried powder.
[0031] It should be noted that other plant extracts can be prepared by the following method, the steps are:
[0032] Remove impurities, grind into powder, add water with a volume ratio of 1:8.5-12 times, soak for 2.5-3 hours, then reflux for 2 hours, filter and collect the filtrate, add water with a volume ratio of 1:5-10 times to the residue again, continue to reflux for 2 hours, filter and collect the filtrate, discard the residue and combine the filtrate, concentrate and evaporate to dryness to obtain the desired extract.
[0033] In the present embodiment, a natural plant extract feed additive with no residue, no pollution, safety and environmental protection is provided. The added Centella asiatica extract, Achyranthes bidentata polysaccharide, Perilla frutescens extract, yeast cell wall extract, Bacillus subtilis freeze-dried powder, and egg yolk oil can resist common pathogens and reduce allergic reactions, thereby controlling the diarrhea caused by bacteria, fungi and nutritional antigens and enhancing immune function. Not only can the damage caused by compounds to animals be avoided, the scientific compounding of Achyranthes bidentata polysaccharide and yeast cell wall extract, Eucommia ulmoides leaf extract, alfalfa extract, coarse old tea extract, ramie root leaf extract and mushroom root extract can effectively reduce the number of antibiotic drugs used in feeding animals, improve the safety of animal meat products, enhance the resistance of animals, and improve feeding efficiency.
[0034] Embodiment 2:
[0035] The difference from the first embodiment is that:
[0036] The invention comprises the following plant extracts in parts by weight: 12 parts of eucommia leaf extract, 12 parts of alfalfa extract, 10 parts of crude old tea leaf extract, 10 parts of ramie root and leaf extract, 10 parts of mushroom root extract, 9 parts of purslane extract, 8 parts of wild chrysanthemum extract, 8 parts of tangerine peel extract, 8 parts of Centella asiatica extract, 26 parts of Achyranthes bidentata polysaccharide, 60 parts of Perilla frutescens extract, 55 parts of yeast cell wall extract, 16 parts of freeze-dried Bacillus subtilis powder, 60 parts of corn flour, 60 parts of bran powder, 85 parts of protein mulberry plant powder and 40 parts of egg yolk oil.
[0037] Embodiment three:
[0038] The difference from the first embodiment is that:
[0039] The invention comprises the following plant extracts in parts by weight: 10 parts of eucommia leaf extract, 8 parts of alfalfa extract, 9 parts of crude old tea extract, 6 parts of ramie root and leaf extract, 8 parts of mushroom root extract, 5 parts of purslane extract, 7 parts of wild chrysanthemum extract, 6 parts of tangerine peel extract, 7 parts of Centella asiatica extract, 20 parts of Achyranthes bidentata polysaccharide, 35 parts of Perilla frutescens extract, 45 parts of yeast cell wall extract, 12 parts of freeze-dried Bacillus subtilis powder, 45 parts of corn flour, 50 parts of bran powder, 78 parts of protein mulberry plant powder and 30 parts of egg yolk oil.
[0040] Table 1, when implemented according to Example 1 to Example 3, the formula composition is as follows:
[0041]
[0042]
[0043] In summary, the present invention can not only resist common pathogens and reduce allergic reactions, thereby controlling diarrhea caused by bacteria, fungi and nutritional antigens and enhancing immune function; it can also effectively reduce the amount of antibiotics used in farmed animals, improve the safety of animal meat products, enhance the resistance of animals, and improve farming efficiency.
[0044] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A fully natural plant extract compound feed additive, characterized in that: The invention comprises the following plant extracts in parts by weight: 35-55 parts of yeast cell wall extract, 3-12 parts of eucommia leaf extract, 5-12 parts of alfalfa extract, 8-10 parts of crude old tea extract, 3-10 parts of ramie root and leaf extract, 6-10 parts of mushroom root extract, 2-9 parts of purslane extract, 1-8 parts of wild chrysanthemum extract, 5-8 parts of tangerine peel extract, 6-8 parts of Centella asiatica extract, 12-26 parts of Achyranthes bidentata polysaccharide, 15-60 parts of Perilla extract, 8-16 parts of freeze-dried Bacillus subtilis powder, 35-60 parts of corn flour, 45-60 parts of bran powder, 70-85 parts of protein mulberry plant powder and 15-40 parts of egg yolk oil.
2. The all-natural plant extract compound feed additive according to claim 1, characterized in that: The invention comprises the following plant extracts in parts by weight: 70 parts of protein mulberry plant powder, 35 parts of corn flour, 45 parts of bran powder, 8 parts of crude old tea extract, 3 parts of eucommia leaf extract, 5 parts of alfalfa extract, 30 parts of ramie root and leaf extract, 6 parts of mushroom root extract, 2 parts of purslane extract, 1 part of wild chrysanthemum extract, 5 parts of tangerine peel extract, 6 parts of Centella asiatica extract, 12 parts of Achyranthes bidentata polysaccharide, 15 parts of Perilla extract, 35 parts of yeast cell wall extract, 8 parts of freeze-dried Bacillus subtilis powder and 15 parts of egg yolk oil.
3. The all-natural plant extract compound feed additive according to claim 1, characterized in that: The invention comprises the following plant extracts in parts by weight: 12 parts of eucommia leaf extract, 12 parts of alfalfa extract, 10 parts of crude old tea leaf extract, 10 parts of ramie root and leaf extract, 10 parts of mushroom root extract, 9 parts of purslane extract, 8 parts of wild chrysanthemum extract, 8 parts of tangerine peel extract, 8 parts of Centella asiatica extract, 26 parts of Achyranthes bidentata polysaccharide, 60 parts of Perilla frutescens extract, 55 parts of yeast cell wall extract, 16 parts of freeze-dried Bacillus subtilis powder, 60 parts of corn flour, 60 parts of bran powder, 85 parts of protein mulberry plant powder and 40 parts of egg yolk oil.
4. The all-natural plant extract compound feed additive according to claim 1, characterized in that: The invention comprises the following plant extracts in parts by weight: 10 parts of eucommia leaf extract, 8 parts of alfalfa extract, 9 parts of crude old tea extract, 6 parts of ramie root and leaf extract, 8 parts of mushroom root extract, 5 parts of purslane extract, 7 parts of wild chrysanthemum extract, 6 parts of tangerine peel extract, 7 parts of Centella asiatica extract, 20 parts of Achyranthes bidentata polysaccharide, 35 parts of Perilla frutescens extract, 45 parts of yeast cell wall extract, 12 parts of freeze-dried Bacillus subtilis powder, 45 parts of corn flour, 50 parts of bran powder, 78 parts of protein mulberry plant powder and 30 parts of egg yolk oil.
5. The all-natural plant extract compound feed additive according to claim 1, characterized in that: The preparation method of the ramie root and leaf extract comprises the following steps: the ramie root and leaf are crushed and passed through a 30-50 mesh sieve, and then a Centella asiatica extract soaking solution is added and heated, the temperature is controlled at 45-60°C, and after 1.5-3 hours, the extract is concentrated and dried to obtain a ramie root and leaf extract, the extract is vacuum concentrated and then spray-dried to obtain a ramie root and leaf extract, and the extract is crushed and sieved to obtain the ramie root and leaf extract.
6. The all-natural plant extract compound feed additive according to claim 4, characterized in that: The preparation method of the eucommia leaf extract comprises the following steps: the eucommia leaves are crushed and passed through a 30-60 mesh sieve, hot water of 2-5 times the weight of the eucommia leaves is added, the extract is collected 2-3 times at intervals, each time for 30-50 minutes, the temperature of the hot water is 70-85°C, ethanol is added to the extract so that the ethanol concentration in the extract reaches 70-85%, the concentration is maintained for 2-3 hours, the precipitate is collected, dried at 60-75°C, crushed, and passed through a 40 mesh sieve to obtain the eucommia leaf extract.
7. The all-natural plant extract compound feed additive according to claim 2, characterized in that: The preparation method of the Achyranthes bidentata polysaccharide comprises the following steps: the Achyranthes bidentata is crushed and passed through a 40-50 mesh sieve, hot water at 70-85°C in an amount 2-4 times the weight of the Achyranthes bidentata is added for continuous extraction for 2-3 times, each time for 30-50 minutes; the extract is collected, ethanol is added to the extract so that the ethanol concentration in the extract reaches 70-85%, and the concentration is maintained for 2-3 hours, the precipitate is collected, dried at 60-75°C, crushed, and passed through a 20-30 mesh sieve to obtain the Achyranthes bidentata polysaccharide.
8. The all-natural plant extract compound feed additive according to claim 4, characterized in that: The preparation method of the Achyranthes bidentata polysaccharide comprises the following steps: removing impurities from protein mulberry, grinding the protein mulberry into powder, adding water with a volume 8 times the volume of the protein mulberry powder, refluxing for 2.5 hours, filtering and collecting the filtrate, adding water with a volume 5 times the volume of the white mulberry powder into the filter residue again, and continuing to reflux for 2 hours; filtering and collecting the filtrate, combining the two filtrates, concentrating and then evaporating to dryness.
9. The all-natural plant extract compound feed additive according to claim 3, characterized in that: The preparation method of the freeze-dried bacillus subtilis powder comprises the following steps: uniformly dispersing a bacillus subtilis suspension in a freeze-drying protective agent solution to form a bacillus subtilis suspension, packaging the suspension in vials, precooling the suspension in an ice water bath, prefreezing the suspension in liquid nitrogen, transferring the precooled and prefrozen bacillus subtilis suspension to a freeze dryer, performing vacuum drying, and obtaining the freeze-dried bacillus subtilis powder.
Citation Information
Patent Citations
Plant extract compound feed additive and production method thereof
CN115997847A