Culture device for high-throughput biological culture system

Abstract

The invention discloses a culture device for a high-flux biological culture system. The culture device comprises a sealed box body with an inlet and an outlet; the C-shaped supporting mechanism is arranged in the box body; the rotating platform mechanism is arranged in the C-shaped supporting mechanism and is used for placing a culture pore plate; the rotating platform mechanism further comprises a bidirectional clamping mechanism arranged on the tray. Compared with an existing cell or organ-like biological culture device, the cell or organ-like biological culture device has the advantages that the culture environment consistency of all pore plates in the culture process is better, and the disturbance to other pore plates is lower when the pore plates are taken; in the culture and observation process, strict environmental conditions can be maintained, the pore plate does not displace, and a more adaptive space moving range is provided for a monitoring and pipetting integrated device with higher observation and analysis speed, so that the requirements of high throughput and low disturbance of a high throughput biological culture system under the industrial and automatic background can be met; technical and theoretical possibilities are provided for successful cell or organoid culture and observation of an intelligent automatic production line.

Description

Technical Field

[0001] The invention relates to a culture device for a high-throughput biological culture system, belonging to the technical field of automated equipment. Background Art

[0002] Automated equipment can accurately complete various fixed mechanical actions and free up manpower, so it is gradually being applied to various industries. The current mainstream solutions for cell or organoid culture and analysis are: biological incubator batch array culture of cells or organoids (see the attached figure of the specification) Fig.10 ), when the well plate needs to be observed and analyzed, the incubator needs to be opened, the layer where the well plate is located is moved out, the robot arm takes out the well plate, the layer where the well plate is located is moved in, the incubator is closed, and the robot arm places the well plate on the living cell analyzer for analysis. After the analysis is completed, the well plate is taken out, the incubator is opened, the robot arm puts the well plate back in place, and another well plate is taken for observation and analysis. The operation is repeated until all the well plates are observed and analyzed. The above mainstream solutions can meet the high-content, low-throughput needs of scientific researchers for cell culture. Among them, the existing living cell analyzers contain a variety of optical analysis devices, the optical path is complex, and the camera lens cannot move over a large range, resulting in a limited number of single observations and analyses, which cannot meet the requirements of industrial high-throughput; this solution requires step-by-step cultivation and analysis, low integration, and high cost; the incubator needs to be opened and closed many times when analyzing the well plate, and the cultivation of cells or organoids requires extremely strict environmental control, which can easily cause contamination to the remaining well plates; and the existing living cell analyzers do not have a well plate positioning mechanism, and scientific researchers need to operate manually during observation, which cannot meet the needs of large-scale cell analysis of automated equipment, and the clamping devices on the market occupy a large space, which will cause spatial interference, or are not flexible clamping devices and cannot meet the needs of multi-model well plate observation. Therefore, the existing technical solutions have defects and need to be improved. Summary of the invention

[0003] The technical purpose of the present invention is to propose a culture device for a high-throughput biological culture system. The present invention analyzes the limitations of existing mainstream cell or organoid culture and analysis solutions under the background of industrial high-throughput requirements, and optimizes the existing array fixed culture layout to a rotating movable culture layout to achieve the goal of keeping the culture environment of all well plates consistent during the culture process, without disturbing the remaining well plates when taking the well plates, maintaining strict environmental conditions during the culture and observation process, and preventing the well plates from being displaced, and providing a more suitable spatial movement range for monitoring and pipetting integrated devices with faster observation and analysis speeds.

[0004] The technical solution adopted by the present invention is:

[0005] A culture device for a high-throughput biological culture system comprises:

[0006] A sealed box with an inlet and outlet;

[0007] A C-shaped support mechanism disposed in the box;

[0008] A rotating platform mechanism disposed in the C-shaped support mechanism for placing a culture well plate;

[0009] The rotating platform mechanism also includes a bidirectional clamping mechanism arranged on the tray.

[0010] The rotating platform mechanism is used for cell or organoid culture.

[0011] When the present invention is actually used, it can be used in conjunction with an existing manipulator or an automatic lifting mechanism or a monitoring mechanism to respectively realize the movement of the orifice plate or the monitoring operation, etc. Alternatively, a sealed box with an opening can be provided to realize the sealing operation.

[0012] A rotating platform mechanism is provided at the center of the C-shaped supporting mechanism.

[0013] Furthermore, the rotating platform mechanism comprises:

[0014] A shaft member fixed in the C-shaped support mechanism;

[0015] A tray disposed on the shaft member;

[0016] A driving mechanism for driving the tray to rotate;

[0017] A plurality of orifice plate placement positions are arranged in a circular array on the tray.

[0018] The orifice plate placement position is generally an orifice plate placement opening structure, and the shape of the orifice plate placement opening corresponds to the shape of the orifice plate.

[0019] Preferably, the rotating platform mechanism further comprises a bidirectional clamping mechanism arranged on the tray, and the bidirectional clamping mechanism comprises a radial clamping assembly for achieving radial clamping of the orifice plate and a circumferential clamping assembly for achieving circumferential clamping of the orifice plate.

[0020] Furthermore, the radial clamping assembly and the circumferential clamping assembly are driven by the same driving mechanism. More specifically, the driving mechanism adopts a steering gear. The orifice plate placement opening is provided with radial limiting edge and circumferential limiting edge structures, which cooperate with the radial clamping assembly and the circumferential clamping assembly to achieve radial and circumferential fixation of the orifice plate.

[0021] Furthermore, the radial clamping assembly includes a radial clamping jaw guide rail, a radial clamping jaw slidably arranged on the radial clamping jaw guide rail, a driving mechanism for driving the radial clamping jaw to release the orifice plate, and a radial elastic member for causing the radial clamping jaw to clamp. Driven by the driving mechanism, the radial clamping jaw slides along the radial clamping jaw guide rail to achieve an unfolding action and release the orifice plate thereon. When clamping is required, the driving mechanism releases the radial clamping jaw, and under the pulling force of the radial elastic member, the inner side of the orifice plate abuts against the radial limiting edge, and the radial clamping jaw achieves the clamping operation on the orifice plate.

[0022] Furthermore, a pulley is provided on one side of the radial clamp for clamping. A positioning protrusion (which may also be a pin) is provided on the radial clamp. A transmission member is provided between the drive mechanism and the radial clamp, and the positioning protrusion is used to fix the transmission member. For example, the transmission member may be a long rope and a plurality of matching guide wheels, and the long rope is wound around the guide wheel and is respectively fastened to the positioning protrusion and the drive mechanism (such as a steering gear). The radial elastic member is a spring, and the two ends of the spring are respectively fastened to the tray and the radial clamp.

[0023] Furthermore, the tray is provided with a fan-shaped opening; the circumferential clamping assembly includes a tangential clamping jaw, a driving mechanism for driving the tangential clamping jaw to release the orifice plate, a tangential swing arm connected between the output end of the driving mechanism and the tangential clamping jaw, and a tangential elastic member for prompting the tangential clamping jaw to clamp the tangential clamping jaw. The tangential swing arm is slidably arranged in the fan-shaped opening, and serves as a transmission member while cooperating with the fan-shaped opening to serve as a guide member. Preferably, the fan-shaped opening is arranged along the circumference of the tray. The fan-shaped opening is used to guide and limit the rotation direction of the tangential swing arm. At the same time, the other end of the tangential swing arm is connected to the tangential elastic member. The tangential clamping jaw is driven to open circumferentially by the driving mechanism; the tangential clamping jaw is retracted circumferentially by the tangential elastic member, and the inner side of the orifice plate abuts against the circumferential limiting edge, so that the tangential clamping jaw clamps the orifice plate.

[0024] Furthermore, the tangential elastic member may be a spiral spring. One end of the tangential swing arm is wound around the center of the spiral spring, and the other end is fixed to the tangential clamping claw through a fan-shaped opening. The tangential swing arm can rotate along the fan-shaped opening under the drive of the spiral spring or the driving mechanism. The transmission member between the driving mechanism and the tangential clamping claw may be a short rope, the two ends of which are respectively fastened to the steering gear and the tangential swing arm, and the tangential clamping claw rotates around the center of the other end of the tangential swing arm.

[0025] The C-shaped support mechanism is used to integrate various sensors and environmental control devices. Further, the C-shaped support mechanism includes a lower base plate, an upper base plate, and a heat sink that fixes the lower base plate and the upper base plate to each other. Furthermore, the rotating platform mechanism is installed between the upper base plate and the lower base plate in the C-shaped support mechanism.

[0026] Specifically, the lower base plate is used to fix the lower center semi-axis, and a lower center semi-axis fixing hole and a line collecting groove α are arranged in the lower base plate, and a humidifier is arranged on the periphery of the lower base plate; a heat sink is arranged behind the lower base plate, and an oxygen vent and a carbon dioxide vent are arranged in the center of the heat sink, a heating rod is arranged on one side of the heat sink, and a sensor fixing plate is arranged on the other side, and a humidity sensor, a carbon dioxide sensor and an oxygen sensor are arranged on the sensor fixing plate; an upper base plate is arranged above the heat sink, and an upper center semi-axis fixing hole and a line collecting groove β are arranged in the upper base plate.

[0027] Further, the shaft member includes an upper center semi-axis and a lower center semi-axis. The upper center semi-axis is coaxially and tightly connected with the lower center semi-axis. The lower center semi-axis and the upper center semi-axis tightly connected thereto are respectively fixed between the lower bottom plate and the upper bottom plate of the C-shaped support mechanism in the culture device. The lower center semi-axis of the rotating platform mechanism in the culture device rotates.

[0028] Furthermore, the top of the upper center semi-shaft is provided with a hole for connecting to the upper base plate of the C-shaped support mechanism, and the middle of the upper center semi-shaft is fastened to the DD motor that drives the tray to rotate. The upper center semi-shaft is fastened concentrically with the lower center semi-shaft, and the lower center semi-shaft is provided with a hole for connecting to the lower base plate of the C-shaped support mechanism.

[0029] More specifically, the rotating platform mechanism includes an upper center semi-axis, and a hole is provided at the top of the upper center semi-axis, which is specifically used to connect to the upper base plate of the C-shaped support mechanism. The middle part of the upper center semi-axis is tightly connected to the DD motor, and the DD motor drives the tray to rotate. Electronic tags are arranged in a circular array around the tray, and these electronic tags are used to record the coding information and temperature data of the orifice plate. Directly above the electronic tag, a wireless scanner is provided to read and write the information in the electronic tag. The upper center semi-axis is concentrically and tightly connected to the lower center semi-axis, and a hole is provided below the lower center semi-axis, and the hole is used to connect to the lower base plate of the C-shaped support mechanism. Through this structural design, the rotating platform mechanism can achieve precise control of the tray and effectively record and read the orifice plate information.

[0030] Preferably, a plurality of orifice plate positions and electronic tags for recording orifice plate information (orifice plate coding information and temperature, etc.) are arranged in a circular array on the tray, and a wireless scanner for reading and writing information in the electronic tags is also arranged in the box.

[0031] The tray is provided with a plurality of electronic tags for recording the orifice plate information in an annular array, and the box is provided with a wireless scanner for reading and writing the information in the electronic tags. Through the above structural design, the rotating platform mechanism can realize the precise control of the tray and effectively record and read the orifice plate information.

[0032] Furthermore, the C-shaped supporting mechanism is provided with a heating unit, a humidifying unit, an oxygen vent and a carbon dioxide vent; the box body is provided with a temperature sensor, a humidity sensor, an oxygen sensor and a carbon dioxide sensor.

[0033] Preferably, the box body is provided with a sliding door mechanism, which includes a baffle plate matched with the entrance and exit, and a baffle plate motor driving the baffle plate to close and open the input port. Furthermore, the output end of the baffle plate motor is fixed to the baffle plate screw slide, and the baffle plate is fixed to the baffle plate screw slide, and moves synchronously with the baffle plate screw slide to realize the door closing and door opening operations.

[0034] Furthermore, the box body includes an upper box body and a lower box body. In actual use, the lower box body is placed on the working platform, and the upper box body is fastened to the lower box body to form a closed space; as an option, the culture device is placed in the lower box body; the plate replacement or liquid transfer is achieved through the entrance and exit. Furthermore, the entrance and exit are provided on the upper box body; and the sliding door mechanism is arranged on the upper box body.

[0035] In the culture device for a high-throughput biological culture system described in the present invention, holes connected to the C-shaped support mechanism are provided on the upper and lower sides of the rotating platform mechanism to realize the rotation drive of the tray and the recording of the well plate information. The C-shaped support mechanism is built with a variety of sensors and environmental control devices to provide suitable environmental conditions for cell or organoid culture. A bidirectional clamping mechanism is arranged around each well plate, and a bidirectional flexible clamping function for well plates of different models can be achieved by controlling the rotation direction and angle of a single servo. Compared with the existing cell or organoid biological culture device, the present invention provides better consistency of culture environment, ensuring that the environmental conditions of all well plates remain uniform during the culture process; when taking any well plate, the disturbance to other well plates is minimized to avoid unnecessary interference to the culture environment; during the entire culture and observation process, strict environmental conditions can be maintained to ensure the stability and reliability of cell or organoid culture; the design provides a more suitable spatial movement range for high-speed monitoring and pipetting integrated devices, thereby improving the speed of observation and analysis; and a two-way flexible clamping mechanism that does not exist in traditional living cell analyzers is added to each well plate, avoiding manual positioning operations, ensuring that the automated equipment can achieve accurate positioning and precise observation when batch observing the well plates. It can meet the new requirements of high-throughput biological incubators in the context of industrialization and automation, and provide technical and theoretical possibilities for the success of intelligent automated production lines for cell or organoid culture and observation.

[0036] Compared with the existing structure, the beneficial effects of the present invention are as follows: the present invention has better consistency of the culture environment of all well plates during the culture process than the existing cell or organoid biological culture device, less disturbance to the other well plates when taking the well plate, can maintain strict environmental conditions during the culture and observation process, the well plate will not be displaced, and provides a more suitable spatial movement range for monitoring and pipetting integrated devices with faster observation and analysis speeds, etc. It can meet the high-throughput and low-disturbance requirements of high-throughput biological culture systems in the context of industrialization and automation, and provides technical and theoretical possibilities for the success of intelligent automated production lines for cell or organoid culture and observation. In order to make the above-mentioned purposes, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0038] Figure 1 It is a schematic diagram of the structure of the culture device in an embodiment of the present invention, in which the box part is omitted.

[0039] Figure 2 yes Figure 1 Structural diagram after removing the upper base plate.

[0040] Figure 3 It is a top view of the rotating platform mechanism.

[0041] Figure 4 It is the front view of the rotating platform mechanism.

[0042] Figure 5 It is a structural diagram of a C-shaped support mechanism.

[0043] Figure 6 This is another structural diagram of the C-shaped support mechanism.

[0044] Figure 7 It is the structural diagram of the bidirectional clamping mechanism.

[0045] Figure 8 It is a top view of the bidirectional clamping mechanism.

[0046] Fig. 9 Bottom view of the bidirectional clamping mechanism.

[0047] Fig.10 It is a structural diagram of an existing biological culture device.

[0048] Fig.11This is an overall diagram of the culture device.

[0049] In the figure

[0050] 1. Rotating platform mechanism; 1-1. Wireless scanner; 1-2. Electronic tag; 1-3. Upper center semi-axis; 1-4. DD motor; 1-5. Tray; 1-6. Lower center semi-axis; 2. C-shaped support mechanism; 2-1. Humidifier; 2-2. Lower base plate; 2-2-1. Lower center semi-axis fixing hole; 2-2-2. Line collecting slot α; 2-3. Heat sink; 2-3-1. Oxygen vent; 2-3-2. Carbon dioxide vent; 2-4 Heating rod; 2-5. Upper base plate; 2-5-1. Upper center semi-axis fixing hole; 2-5-2. Line collecting slot β; 2-6. Humidity sensor; 2-7. Sensor fixing plate; 2-8. Carbon dioxide sensor; 2-9. Oxygen sensor. 3. Bidirectional clamping mechanism; 3-1. Radial clamping jaw; 3-1-1. Pulley α; 3-1-2. Pin α; 3-2. Pin β; 3-3. Guide wheel α; 3-4. Long rope; 3-5. Pin γ; 3-6. Guide wheel β; 3-7. Servo; 3-8. Short rope; 3-9. Vortex spring; 3-10. Tangential swing arm; 3-11. Fan-shaped opening; 3-12. Tangential clamping jaw; 3-12-1. Pulley β; 3-13. Tension spring; 3-14. Radial clamping jaw guide rail; 4. Box; 4-1. Upper box; 4-2. Lower box; 5. Sliding door mechanism; 5-1. Baffle; 5-2. Baffle screw slide; 5-3. Baffle motor; 6. Entrance and exit. DETAILED DESCRIPTION

[0051] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0052] like Figure 1 and Fig.11 As shown, in this embodiment, a culture device for a high-throughput biological culture system includes a box body 4, a C-shaped support mechanism 2 arranged in the box body 4, and a rotating platform mechanism 1 and a bidirectional clamping mechanism 3 arranged in the C-shaped support mechanism 2.

[0053] like Figures 2 to 4As shown, in this embodiment, the rotating platform mechanism 1 includes an upper center semi-axis 1-3, a lower center semi-axis 1-6, a tray 1-5, a DD motor 1-4, etc. The middle of the upper center semi-axis is fastened to the DD motor 1-4 and is connected to the tray 1-5, and the rotation of the tray 1-5 is driven by the DD motor 1-4. The tray 1-5 is evenly provided with a plurality of orifice plate placement openings 1-7 in the circumferential direction; the tray 1-5 is provided with electronic tags 1-2 corresponding to the corresponding orifice plates around it, and a wireless scanner 1-1 for scanning and reading the information of the electronic tags 1-2 is provided above the tray 1-5; the upper center semi-axis 1-3 is fastened to the lower center semi-axis 1-6.

[0054] like Figures 5-6 As shown, in this embodiment, the C-shaped support mechanism 2 is mainly used to provide installation space. The C-shaped support mechanism 2 includes an upper base plate 2-5, a lower base plate 2-2, and a heat sink 2-4 that fixes the upper base plate 2-5 and the lower base plate 2-2 to each other. Among them, the lower base plate 2-2 is provided with a lower center semi-axis fixing hole 2-2-1 and a line collection groove α2-2-2, and a humidifier 2-1 is provided on the periphery of the lower base plate 2-2. A heat sink 2-3 is provided behind the lower base plate 2-2. An oxygen vent 2-3-1 and a carbon dioxide vent 2-3-2 are provided in the center of the heat sink 2-3; a heating rod 2-4 is provided on the left side of the heat sink 2-3, and a sensor fixing plate 2-7 is provided on the right side. A humidity sensor 2-6, a carbon dioxide sensor 2-8, and an oxygen sensor 2-9 are provided on the right side of the sensor fixing plate. An upper base plate 2-5 is provided above the heat sink, and an upper center semi-axis fixing hole 2-5-1 and a line collection groove β2-5-2 are provided in the upper base plate. At the same time, the electronic tag has a temperature detection function. Through the electronic tag temperature detection function and the cooperation of the heating rod 2-4, the temperature in the box can be accurately controlled. The humidifier 2-1 and the humidity sensor 2-6 can be used to accurately control the humidity in the box. The carbon dioxide sensor 2-8 and the oxygen sensor 2-9 can detect and control carbon dioxide and oxygen in the box.

[0055] like Figures 7 to 9As shown, in this embodiment, it is arranged at a position corresponding to the orifice plate placement opening 1-7 on the tray 1-5. The bidirectional clamping mechanism 3 includes a radial clamping claw guide 3-14 arranged along the radial direction of the tray 1-5, and a radial clamping claw 3-1 arranged on the radial clamping claw guide 3-14 and capable of sliding along the radial clamping claw guide 3-14; the radial clamping claw 3-1 is in the shape of an elongated strip, and the length direction is arranged along the vertical radial direction, and is mainly used for radial positioning of the orifice plate; a pulley α3-1-1 is provided on the inner side of the radial clamping claw 3-1, and a pin α3-1-2 is provided on the upper side. The tray 4-5 is also provided with a pin β3-2, a pin γ3-5 and a steering gear 3-7, the steering gear 3-7 is a driving mechanism, and the transmission member is a long rope 3-4. The pin β3-2 and the pin γ3-5 are provided with a guide wheel α3-3 and a guide wheel β3-6 respectively. One end of the long rope 3-4 is fixed to the output end of the steering gear 3-7, and the other end is passed through the guide wheel α3-3 and the guide wheel β3-6 and fastened to the pin α3-1-2. The two ends of a radially arranged tension spring 3-13 are fastened to the tray 4-5 and the radial clamp 3-1 respectively, and are stretched toward the center axis direction of the tray 4-5. A tangential clamp 3-12 is also provided on the tray 4-5 for realizing circumferential positioning of the orifice plate; a pulley β3-12-1 is provided on the inner side of the tangential clamp 3-12; a fan-shaped opening 3-11 and a tangential swing arm 3-10 arranged along the circumference are also provided on the tray 4-5, one end of the tangential swing arm 3-10 is connected to the center of the spiral spring 3-9, and the other end passes through the fan-shaped opening 3-11 arranged on the tray and is connected to the tangential clamp 3-12, driving the tangential clamp 3-12 to rotate synchronously; one end of the tangential swing arm 3-10 connected to the spiral spring 3-9 is fastened to the output end of the steering gear 3-7 through a short rope 3-8, and the other end is fastened to the tangential swing arm 3-10, and the tangential clamp 3-12 rotates around the center of the connection end of the tangential swing arm 3-10 and the spiral spring.

[0056] In the bidirectional clamping mechanism 3, the radial jaws 3-1, the radial jaw guide rails 3-14 and the tension spring 3-13 form a radial positioning assembly, and the tangential jaws 3-12 and the spiral spring 3-9 cooperate with the guidance of the fan-shaped opening 3-11 to form a circumferential positioning assembly; the servo 3-7 provides driving force for the radial jaws 3-1 and the tangential jaws 3-12 at the same time, which are respectively used to realize the radial outward opening of the radial jaws 3-1 and the circumferential outward opening of the tangential jaws 3-12; the radial jaws 3-1 simultaneously use the tension spring 3-13 to realize the radial inward clamping of the orifice plate; the tangential jaws 3-12 realize the circumferential clamping of the orifice plate under the torsion of the spiral spring 3-9.

[0057] At the same time, radial limiting edges and circumferential limiting edges are provided at the orifice plate placement opening, which cooperate with the radial clamping assembly and the circumferential clamping assembly to achieve radial and circumferential fixation of the orifice plate. When clamping is required, the driving mechanism releases the radial clamping jaws, and under the pulling force of the radial elastic member, the inner side of the orifice plate abuts against the radial limiting edge, and the radial clamping jaws achieve the clamping operation on the orifice plate. At the same time, the driving mechanism releases the tangential clamping jaws, and the tangential elastic member retracts the tangential clamping jaws circumferentially inward, and the inner side of the orifice plate abuts against the circumferential limiting edge, thereby achieving the clamping of the orifice plate by the tangential clamping jaws. Fig.11 As shown, in this embodiment, the box body 4 includes an upper box body 4-1 and a lower box body 4-2, and the culture devices are placed in the lower box body 4-2. The upper box body 4-1 is fastened to the lower box body 4-2 to form a closed space. A sliding door mechanism 5 device for controlling the box body plate changing or lifting port switch is provided on the upper left side of the upper box body, and a plate changing or liquid changing inlet and outlet 6 is provided on the top of the upper box body.

[0058] The sliding door mechanism 5 is mainly composed of a baffle 5-1, a baffle screw slide 5-2 and a baffle motor 5-3. A door structure is provided on the upper box body 4-2. The baffle 5-1 corresponds to the above-mentioned door structure and is used to close and open the box body 4. The other end of the baffle 5-1 is fixed to the baffle screw slide 5-2 and can move synchronously. The baffle screw slide 5-2 is connected to the output end of the baffle motor 5-3.

[0059] In this embodiment, when in use, the operation process is as follows: first turn on the power, then activate the humidifier 2-1, the heating rod 2-4, the oxygen vent 2-3-1 and the carbon dioxide vent 2-3-2 to start adjusting the environment inside the incubator. At the same time, the humidity sensor 2-6, the electronic tag 1-2 with temperature sensing and recording functions, the oxygen sensor 2-3-1 and the carbon dioxide sensor 2-3-2 will feed back the real-time monitoring data to the host computer. After an appropriate period of time, the internal environment of the incubator will meet the culture conditions of cells or organoids. Subsequently, the host computer commands the DD motor 1-4 on the upper center semi-axis 1-3 to drive its tray 1-5 to rotate. At the same time, the servo 3-7 pulls the long rope 3-4 and the short rope 3-8 to drive the radial jaws 3-1 and the tangential jaws 3-12 to open outward. The robotic arm places the well plate on the rotating tray 1-5 in turn, the servo 3-7 rotates in the opposite direction, the tension spring 3-13 and the scroll spring 3-9 release the elastic potential energy, and drive the radial clamp 3-1 and the tangential clamp 3-12 inward to tighten the well plate. At this time, the wireless scanner 1-1 on the upper base plate 2-5 writes the well plate information (including the time of entering the box, the duration of culture, the number of times the liquid is changed, the survival status of cells or organoids, etc.) into the electronic tag 1-2 of each well plate, and then starts the culture program of cells or organoids. When changing the well plate or replacing the well plate, the bidirectional clamping mechanism 3 controls the opening and closing of the clamp, and the robotic arm will accurately move out the required well plate without disturbing other well plates. After the operation is completed, the well plate is reset and the tray 1-5 continues to rotate. When the electronic tag 1-2 is aligned with the bottom of the wireless scanner 1-1, the wireless scanner 1-1 will complete the update of the information. After the update, the culture process of cells or organoids continues.

[0060] Unless otherwise stated, any technical solution disclosed in the present invention disclosed above, if it discloses a numerical range, then the disclosed numerical range is a preferred numerical range, and any technician in the field should understand that the preferred numerical range is only a numerical value with a more obvious technical effect or representative value among many implementable numerical values. Since there are too many numerical values ​​to be exhaustive, the present invention discloses some numerical values ​​to illustrate the technical solution of the present invention, and the numerical values ​​listed above should not constitute a limitation on the scope of protection of the present invention.

[0061] At the same time, if the above-mentioned invention discloses or involves components or structural parts that are fixedly connected to each other, then, unless otherwise stated, the fixed connection can be understood as: a detachable fixed connection (for example, connection using bolts or screws), and can also be understood as: a non-detachable fixed connection (for example, riveting, welding). Of course, the mutual fixed connection can also be replaced by an integrated structure (for example, manufactured by one-piece molding using a casting process) (except when it is obviously impossible to use an one-piece molding process).

[0062] If the words "first", "second", etc. are used in this article to limit components, those skilled in the art should know that the use of "first" and "second" is only to distinguish the components for the convenience of description. Unless otherwise stated, the above words have no special meaning.

[0063] When "α", "β", "γ" and "δ" are used as suffixes to define component names in this article, it is only for the convenience of distinguishing the components for the purpose of description; unless otherwise stated, the above words have no special meaning and no other limiting effect.

[0064] In addition, unless otherwise stated, the terms used to indicate positional relationships or shapes in any of the technical solutions disclosed in the present invention include states or shapes that are approximate, similar, or close to them.

[0065] Any component provided by the present invention can be assembled from multiple separate components, or can be a separate component manufactured by an integral forming process.

[0066] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, ordinary technicians in the field should understand that the specific implementation methods of the present invention can still be modified or some technical features can be replaced by equivalents without departing from the spirit of the technical solution of the present invention, which should be included in the scope of the technical solution for protection of the present invention.

Claims

1. A culture device for a high-throughput biological culture system, characterized in that: include: A sealed box with an inlet and outlet; A C-shaped support mechanism disposed in the box; A rotating platform mechanism disposed in the C-shaped support mechanism for placing a culture well plate; The rotating platform mechanism also includes a bidirectional clamping mechanism arranged on the tray.

2. The culture device for a high-throughput biological culture system according to claim 1, characterized in that: The rotating platform mechanism comprises: A shaft member fixed in the C-shaped support mechanism; A tray disposed on the shaft member; A driving mechanism for driving the tray to rotate; A plurality of orifice plate placement positions are arranged in a circular array on the tray.

3. The culture device for a high-throughput biological culture system according to claim 2, characterized in that: The bidirectional clamping mechanism comprises a radial clamping assembly for realizing radial clamping of the orifice plate and a circumferential clamping assembly for realizing circumferential clamping of the orifice plate.

4. The culture device for a high-throughput biological culture system according to claim 3, characterized in that: The radial clamping assembly and the circumferential clamping assembly are driven by the same driving mechanism.

5. The culture device for a high-throughput biological culture system according to claim 3, characterized in that: The radial clamping assembly comprises a radial clamping jaw guide rail, a radial clamping jaw slidably arranged on the radial clamping jaw guide rail, a driving mechanism for driving the radial clamping jaw to release the orifice plate, and a radial elastic member for causing the radial clamping jaw to clamp.

6. The culture device for a high-throughput biological culture system according to claim 3, characterized in that: A fan-shaped opening is provided on the tray; the circumferential clamping assembly includes a tangential clamping jaw, a driving mechanism for driving the tangential clamping jaw to release the orifice plate, a tangential rotary arm connected between the output end of the driving mechanism and the tangential clamping jaw, and a tangential elastic member that prompts the tangential clamping jaw to clamp the tangential clamping jaw. The tangential rotary arm is slidably arranged in the fan-shaped opening, and while serving as a transmission member, it also serves as a guide member in conjunction with the fan-shaped opening.

7. The culture device for a high-throughput biological culture system according to claim 2, characterized in that: A plurality of electronic tags for recording the orifice plate information are arranged in a circular array on the tray, and a wireless scanner for reading and writing the information in the electronic tags is also arranged in the box.

8. The culture device for a high-throughput biological culture system according to claim 1, characterized in that: The C-shaped supporting mechanism is provided with a heating unit, a humidifying unit, an oxygen vent and a carbon dioxide vent; the box body is provided with a temperature sensor, a humidity sensor, an oxygen sensor and a carbon dioxide sensor.

9. The culture device for a high-throughput biological culture system according to claim 1, characterized in that: The C-shaped supporting mechanism comprises a lower base plate, an upper base plate and a heat dissipation plate which fixes the lower base plate and the upper base plate to each other.

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