Acetobacter grifonali JK-23 and application thereof in oxidation resistance and inflammation resistance

Abstract

The invention discloses a strain of acetobacter grifanii JK-23 and application thereof in antioxidation and anti-inflammation, the strain is preserved in China Center for Type Culture Collection on July 3, 2024, and the preservation number is CCTCC NO: M 20241457. The strain has significant probiotic characteristics, has good antioxidant and anti-inflammatory capabilities, and can significantly relieve H2O2-induced IPEC-J2 cell oxidative damage. The traditional Chinese medicine composition can be used for remarkably relieving the reduction of colon length caused by DSS, colitis sexual infiltration, the reduction of antioxidant indexes CAT and SOD in serum, the abnormal increase of MDA and MPO in the serum and the abnormal increase of the expression quantity of pro-inflammatory TNF-alpha, IL-10, IL-6 and IL-12 in the serum and colon; the strain can be used as a microbial drug or product with a good industrial prospect for treating or relieving cell oxidative damage and colitis, and has potential clinical application value.

Description

Technical Field

[0001] The invention belongs to the field of microorganisms, and in particular relates to a strain of Acetobacter ghanaensis JK-23 and application thereof in anti-oxidation and anti-inflammation. Background Art

[0002] In recent years, both at home and abroad, the role of intestinal flora in human health has been continuously explored. There is a symbiotic dependence relationship between intestinal flora and human hosts, with mutual influence and interaction. Studies have shown that intestinal flora ecological imbalance can affect the development and dysfunction of many systems such as the human nervous system, digestion, metabolism, and immunity, leading to the occurrence of diseases. Therefore, maintaining the balance of intestinal flora is crucial to maintaining human health. A large number of experiments have shown that probiotics have the effects of inhibiting the growth of harmful bacteria, promoting the reproduction of beneficial bacteria, regulating immune responses, and improving intestinal barrier function. Acetic acid bacteria is a kind of probiotics. In the existing technology, it is mainly used for industrial fermentation and research on its effects on alcoholic liver. In the existing literature (Jiang Xinxin. Research on the protective effect and mechanism of xylem acetobacter on alcoholic liver damage [D]. Shaanxi Normal University, 2019.; Qu Wei, Wang Yuhong, Jiang Xiuyu, et al. Optimization of acetic acid fermentation process of birch sap vinegar by response surface methodology [J]. Forestry Science and Technology, 2024, 49(03): 41-46.; Ji Yudan, Wu Dianhui, Yang Fan, et al. Breeding of acetic acid bacteria suitable for brewing green plum vinegar [J]. Journal of Food and Biotechnology, 2024, 43(05): 81-90.), there are public reports on the optimization of acetic acid fermentation process of birch sap vinegar by response surface methodology, breeding of acetic acid bacteria suitable for brewing green plum vinegar, and research on the protective effect and mechanism of xylem acetobacter on alcoholic liver damage, but there are no reports on its effects on alleviating intestinal oxidative stress and inflammatory bowel disease. Summary of the invention

[0003] Purpose of the invention: In order to solve the above technical problems, the present invention aims to provide a strain of Acetobacter Ghana JK-23, which is isolated from traditional fermented dairy products in Kezhou, Xinjiang, and has the effect of treating or alleviating cell oxidative damage and colitis, and can effectively alleviate intestinal mucosal damage, intestinal atrophy and bloody stool symptoms, and provide a good source of bacteria for further development and application of probiotic preparations and relief of intestinal oxidative stress and inflammatory bowel disease.

[0004] Another object of the present invention is to provide the use of Acetobacter Ghana JK-23 in treating or alleviating cell oxidative damage, intestinal mucosal damage, intestinal atrophy, bloody stool and colitis.

[0005] Technical solution: In order to achieve the above purpose, the strain of Acetobacter ghanensis JK-23 described in the present invention has been deposited in the China Center for Type Culture Collection on July 3, 2024, with the deposit address being Wuhan University, China, and the deposit number being CCTCCNO: M20241457. The classification name is: Acetobacter ghanensis strain JK-23.

[0006] The Acetobacter Ghana JK-23 of the present invention is used in the preparation of medicines, reagents, functional foods, special medical formula foods or health products for treating or alleviating cell oxidation damage, intestinal mucosal damage, intestinal atrophy, blood in stool and colitis.

[0007] The invention discloses an application of the Acetobacter ghanaiensis JK-23 in the preparation of a medicine, a reagent, a functional food, a special medical formula food or a health product for treating or alleviating H2O2-induced oxidative damage to IPEC-J2 cells.

[0008] The invention discloses an application of the Acetobacter ghanianus JK-23 in the preparation of a drug, reagent, functional food, special medical formula food or health product for treating or alleviating DSS-induced colitis.

[0009] Furthermore, the Acetobacter Ghanaiensis JK-23 treats or alleviates DSS-induced colitis by changing the length of the colon and the structure of the colon tissue, while reducing the levels of inflammatory factors in serum and colon tissue and increasing the levels of antioxidant stress factors.

[0010] The Acetobacter Ghana JK-23 of the present invention is used in the preparation of medicines, reagents, functional foods, special medical formula foods or health products for treating or alleviating oxidative damage to human or animal cells, intestinal mucosal damage, intestinal atrophy, blood in stool and colitis.

[0011] Furthermore, the animals include pigs, chickens, ducks, fish, mice, etc.

[0012] The bacterial agent produced by Acetobacter ghanaensis JK-23 of the present invention is used for treating or alleviating cell oxidation damage, intestinal mucosal damage, intestinal atrophy, bloody stool and colitis.

[0013] Furthermore, the anti-inflammatory bacterial agent is used to treat and alleviate H2O2-induced IPEC-J2 cell oxidative damage and DSS-induced colitis.

[0014] Furthermore, the bacterial agent contains the Acetobacter ghanaianus JK-23 and / or the culture of Acetobacter ghanaianus JK-23.

[0015] Furthermore, the culture is a bacterial suspension obtained by culturing the Acetobacter Ghana JK-23 and then adding physiological saline to resuspend the bacterial cells, or a cell-free extract of Acetobacter Ghana JK-23.

[0016] Furthermore, the content of Acetobacter Ghana JK-23 in the anti-inflammatory bacterial agent is not less than 1×10 9 CFU / mL.

[0017] Beneficial effects: Compared with the prior art, the present invention has the following significant advantages:

[0018] The isolated and screened strain of Acetobacter ghanai JK-23 of the present invention has significant prebiotic properties, good antioxidant and anti-inflammatory abilities, can significantly alleviate H2O2-induced oxidative damage to IPEC-J2 cells, can significantly alleviate shortening of colon length, inflammatory infiltration of colon, decrease of antioxidant indicators CAT and SOD in serum, abnormal increase of MDA and MPO in serum, and abnormal increase of pro-inflammatory factors TNF-α, IL-10, IL-6 and IL-12 expression in serum and colon caused by DSS. The present invention also provides a study on the alleviating effect of JK-23 on DSS-induced colitis in mice, indicating that JK-23 can effectively improve DSS-induced enteritis symptoms such as weight loss, diarrhea and bloody stools, and has good prebiotic properties. The Acetobacter Ghanaiensis JK-23 of the present invention has the effect of alleviating H2O2-induced IPEC-J2 cell oxidative damage and DSS-induced colitis, can effectively alleviate intestinal mucosal damage, intestinal atrophy and bloody stool symptoms, can be further developed and applied for probiotic preparations and relief of inflammatory bowel disease and provide a good strain source, can be used as a microbial drug or product with good industrial prospects, and has potential clinical application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 The colony morphology and bacterial morphology of JK-23, where (a) is the colony morphology and (b) is the bacterial morphology;

[0020] Figure 2 This is the molecular biological identification diagram of JK-23 strain;

[0021] Figure 3 The effect of JK-23 strain on the morphology and number of IPEC-J2 cells, a cell model of oxidative damage induced by H2O2, where A is the blank control group, B is the H2O2 group, and C is the JK-23+H2O2 group;

[0022] Figure 4 The effect of JK-23 on the survival rate of IPEC-J2 cells, a cell model of oxidative damage induced by H2O2;

[0023] Figure 5The effect of JK-23 on the weight change of mice, compared with the Control group, * indicates P < 0.05, ** indicates P < 0.01; compared with the DSS modeling group, # indicates P < 0.05, ## indicates P < 0.01;

[0024] Figure 6 The effect of JK-23 on the DAI score of mice, where compared with the Control group, * indicates P < 0.05, ** indicates P < 0.01; compared with the DSS model group, # indicates P < 0.05, ## indicates P < 0.01;

[0025] Figure 7 The effect of JK-23 on the colon length of mice, where (A) is a comparison chart of colon length of each group, and (B) is a bar graph of colon length of each group; compared with the Control group, * indicates P < 0.05, ** indicates P < 0.01; compared with the DSS modeling group, # indicates P < 0.05, ## indicates P < 0.01;

[0026] Figure 8 The effect of JK-23 on the colon tissue structure of mice, where the black arrows indicate the loss of intestinal crypts, intestinal mucosal epithelium and intestinal glands, the white asterisks indicate inflammatory cell infiltration, the yellow arrows indicate the loss and irregular arrangement of glands, and the green arrows indicate the defect of colon mucosal epithelial cells;

[0027] Fig. 9 The effect of JK-23 on inflammatory factors in mouse serum, compared with the Control group, * indicates P < 0.05, ** indicates P < 0.01; compared with the DSS modeling group, # indicates P < 0.05, ## indicates P < 0.01;

[0028] Fig.10 The effect of JK-23 on inflammatory factors in the colon tissue of mice, compared with the Control group, * indicates P < 0.05, ** indicates P < 0.01; compared with the DSS model group, # indicates P < 0.05, ## indicates P < 0.01;

[0029] Fig.11 The effect of JK-23 on oxidative stress factors in mouse serum. Compared with the Control group, * indicates P < 0.05, ** indicates P < 0.01; compared with the DSS model group, # indicates P < 0.05, ## indicates P < 0.01. DETAILED DESCRIPTION

[0030] The technical solution of the present invention is further described below in conjunction with the accompanying drawings.

[0031] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources. Experimental methods without specific conditions specified in the examples are usually carried out under conventional conditions or under conditions recommended by the manufacturer.

[0032] The experimental data of the present invention were processed and plotted using SPSS17.0 and GraphpadPrism 9, and the significance analysis was performed using One-Way ANOVA in SPSS17.0, with P < 0.05 indicating significant differences. The 16S rRNA sequencing results of the strains were homologously compared in the NCBI database, and a phylogenetic tree was constructed using MAGE 10.0 software.

[0033] Example 1

[0034] Identification of Acetobacter ghanaense JK-23

[0035] 1. Colony characteristics

[0036] A strain of bacteria was isolated from the traditional fermented mare's milk wine of herders in Kezhou, Xinjiang, and named JK-23. Figure 1 As shown in (a), in YPD agar medium, the colony is round, smooth on the surface, slightly raised in the middle, light white, and shiny. Figure 1 As shown in (b), the experimental strain is Gram-negative, and the bacterial morphology is short rod-shaped, arranged singly, in pairs or in a few chains.

[0037] 2. Identification of strain 16SrDNA

[0038]

[0039] The sequencing results were compared for homology in the NCBI database. The strain had a very high homology with the Acetobacter ghanaensis published in NCBI. The phylogenetic tree was constructed based on the sequencing results by the neighbor-joining method. The phylogenetic tree was constructed by MAGE10.0 software, such as Figure 2 As shown, the strain has a very high homology with the Acetobacter ghanensis strain published on NCBI. The strain was identified as Acetobacter ghanensis strain and named Acetobacter ghanensis strain JK-23.

[0040] The strain Acetobacter ghana JK-23 of the present invention has been deposited in the China Center for Type Culture Collection on July 3, 2024, with the deposit address being Wuhan University, China, and the deposit number being CCTCC NO: M 20241457.

[0041] 3. Strain isolation and purification

[0042] The frozen tube of Ghana Acetobacter JK-23 preserved with glycerol was thawed at room temperature, and the Ghana Acetobacter JK-23 participating in the experiment was inoculated into the prepared YPD solid medium, and then the Ghana Acetobacter JK-23 was revived using the YPD solid medium and cultured in a 37°C incubator for 24 hours. After the strain was inoculated on the solid medium for 3 generations, the strain was observed under a microscope, and after confirming that there was no contamination by other bacteria, it was inoculated into the YPD solid medium for culture and set aside.

[0043] Example 2

[0044] 1. Preparation of bacterial suspension and cell-free extract

[0045] The Acetobacter Ghana JK-23 obtained in Example 1 was cultured to the logarithmic phase and the bacterial solution was centrifuged at 4°C and 6000 r / min for 10 min to obtain a bacterial precipitate. The bacterial precipitate was washed three times with a sterile saline solution and resuspended in a saline solution to adjust the concentration to 10 9 CFU / mL, divided into two parts, one as a bacterial suspension for oral gavage of mice, and the other stored at -20℃, thawed and ultrasonically disrupted in an ice bath with a cell disruptor (150W, ultrasound 5s, interval 10s, disruption 20min), repeated freeze-thaw and ultrasonic disruption 3 times, centrifuged at 4℃ and 12000r / min for 10min, and the supernatant was collected as the JK-23 cell-free extract, which was used to alleviate H2O2-induced oxidative damage of IPEC-J2 cells.

[0046] 2. Establishment of H2O2 oxidative stress model and evaluation of JK-23 antioxidant activity in vitro

[0047] Experimental groups: control group (group C), model group (H2O2 group), JK-23+H2O2 group (10 9 CFU / mL of cell-free extract of bacterial solution + H2O2).

[0048] Group C: After IPEC-J2 cells were cultured for 24 h and adhered to the wall, the culture medium was changed to complete medium without adding strain lysis buffer and without H2O2 damage treatment;

[0049] H2O2 group: After IPEC-J2 cells were cultured for 24 hours and adhered to the wall, the culture medium was changed to complete medium, and H2O2 was added to the concentration of 400 μmol / L for 3 hours after 6 hours;

[0050] JK-23+H2O2 group: After IPEC-J2 cells were cultured for 24 hours and attached to the wall, the cells were replaced with 10 9 The culture medium of JK-23 cell-free extract with CFU / mL bacterial liquid was pretreated for 6 hours, and then 400 μmol / L H2O2 was added for 3 hours.

[0051] The cells were seeded in 12-well plates (1 ml / well, 2.0 × 10 5 / ml) or 96-well plate (0.1ml / well, 1.0×10 5 After the cell culture was completed, the 12-well plate was used to observe the cell morphology and take pictures under an inverted microscope. The 96-well plate was used to complete the cell activity test according to the CCK-8 cell proliferation-toxicity detection kit.

[0052] like Figure 3 As shown in the figure, compared with the control group, the number of cells in the H2O2 group was significantly reduced, the cell morphology changed from long spindle to wrinkled, oblate or even elliptical, and the cell nucleus was significantly enlarged; compared with the H2O2 group, the number of cells in the H2O2+JK-23 group increased, and the shrinkage and other morphological changes of the cells were alleviated. Figure 4 As shown, compared with the control group, the cell survival rate in the H2O2 group was significantly decreased (P < 0.01); compared with the H2O2 group, the cell survival rate in the H2O2+JK-23 group was significantly increased to 84.59% (P < 0.05), and there was no significant difference in cell survival rate compared with the control group (P > 0.05).

[0053] It can be seen from the above experimental results that the Acetobacter Ghana JK-23 or its cell-free extract provided by the present invention can greatly alleviate H2O2-induced oxidative damage to IPEC-J2 cells, providing a new option for the intervention of intestinal oxidative stress.

[0054] Example 3

[0055] 1. Establishment and grouping of colitis animal model

[0056] Eighteen mice were randomly divided into three groups (n=6): Control group, DSS modeling group, and JK-23+DSS group. During the entire experiment, mice in each group were adapted for one week. The Control group and DSS modeling group received normal saline (0.1 mL / 10 g mouse body weight) by gavage every day for 14 consecutive days, and they were free to eat during this period. The mice in the JK-23+DSS group received a suspension of Acetobacter Ghana JK-23 (1×10 9 CFU / mL, 0.1mL / 10g mouse body weight) for 14 consecutive days, daily gavage, free diet during the period. In the next week, the mice in the Control group had free drinking water, the DSS modeling group had free drinking of 3% DSS aqueous solution, and the JK-23+DSS group had free drinking of 3% DSS aqueous solution, and free diet during the period. The weight, vitality, feces, and anal bleeding of the mice were recorded on time, and the bleeding of the mice was observed in time after gavage every day. After 3 weeks of intervention, the mice that had fasted for 24 hours were euthanized to collect the colon, and the colon length was recorded.

[0057] 2. Disease Activity Index (DAI) Scoring of Mice

[0058] During the construction of the mouse enteritis model, the DAI scoring method in the reference (Yang Yang, Zhou Xiechen, Li Tao, Li Yan, Cao Junyang, Zhao Rui. Preparation and evaluation of the Kunming mouse model of acute ulcerative colitis [J]. Advances in Veterinary Medicine, 2023, 44(02): 72-77.) was used to formulate the DAI scoring table (Table 1) for scoring, DAI = (weight loss score + blood in stool score + stool characteristics score) / 3. Figure 5 As shown in the figure, the weight of mice in the Control group continued to increase during the experiment, while the weight of the other two groups decreased, especially from the fourth day onwards. Compared with the Control group, the weight of the DSS group was significantly lower (P < 0.01) than that of the Control group, and compared with the DSS group, the weight of the JK-23 + DSS group was significantly higher (P < 0.01) than that of the DSS group. Figure 6 The results showed that the scores of the DSS modeling group and the JK-23+DSS group continued to rise during the DSS modeling experiment. Compared with the Control group, the scores of the DSS group were significantly higher (P < 0.01) than those of the Control group; compared with the DSS group, the scores of the JK-23+DSS group were significantly lower (P < 0.01) than those of the DSS group. Figure 7As shown, the longest colon length of the Control group was 5.5±0.58cm, the colon length of the DSS modeling group was 4.3±0.36cm, and the colon length of the JK-23+DSS group was 4.6±0.65cm. Compared with the Control group, the colon length of the DSS group was significantly lower (P<0.05) than that of the Control group; compared with the DSS group, the colon length of the JK-23+DSS group was significantly higher than that of the DSS group.

[0059] From the above experimental results, it can be seen that the Acetobacter Ghana JK-23 provided by the present invention can greatly improve the DAI score of mice, can well alleviate DSS-induced colitis in mice, and provide a new option for the treatment of colitis.

[0060] Table 1 Disease Activity Index (DAI score)

[0061]

[0062] 3. Histopathological examination

[0063] The distal colon of mice in each treatment group in step 2 was collected and fixed in formalin. The tissue sections were stained with HE and observed under a microscope. Figure 8 As shown in the figure, the colon mucosal epithelial cells of the Control mice were intact, the crypts were intact and the morphology was normal, the glands and goblet cells were neatly arranged, and there was no inflammatory cell infiltration. Compared with the control group, the colon of the DSS group mice had no complete mucosal tissue structure, and the intestinal crypts, intestinal mucosal epithelium and intestinal glands were largely missing (indicated by the black arrows), and the inflammatory cell infiltration was obvious (indicated by the white asterisks). Compared with the DSS group, the colon mucosal tissue structure of the JK-24+DSS group mice was basically intact, the glands and goblet cells were relatively neatly arranged in most areas, the inflammatory cell infiltration area was significantly reduced, and only a small number of areas had gland loss and irregular arrangement (indicated by the yellow arrows), and some areas had mild mucosal epithelial cell defects (indicated by the green arrows).

[0064] It can be seen from the above experimental results that the Acetobacter Ghana JK-23 provided by the present invention can effectively alleviate DSS-induced colitis in mice, improve colon length and colon tissue structure, and has great clinical research value.

[0065] 4. Detection of inflammatory factors in mouse colon tissue and serum

[0066] Blood samples were collected from mice in each treatment group after enucleation in step 2, and the samples were centrifuged at 4000 rpm for 10 min to obtain serum. Serum was aspirated and stored at -80°C for later use. TNF-α, IL-6, IL-12, and IL-10 inflammatory factor ELISA detection kits were used to determine the content of inflammatory factors in serum according to the instructions of the kit. Fig. 9As shown, it can be seen that compared with the Control group, the TNF-α, IL-10 and IL-6 in the DSS group were significantly higher (P < 0.01) than those in the Control group, and the IL-12 in the DSS group was significantly higher (P < 0.05) than that in the Control group; compared with the DSS group, the TNF-α and IL-10 in the JK-23+DSS group were significantly lower (P < 0.01) than those in the DSS modeling group, and the IL-6 and IL-12 in the JK-23+DSS group were significantly lower (P < 0.05) than those in the DSS modeling group.

[0067] Take the colon tissue samples of mice in each treatment group in step 2 and place them in cryopreservation tubes. Quickly freeze them in liquid nitrogen and store them at -80°C for later use. Use TNF-α, IL-6 and other inflammatory factor ELISA detection kits and determine the content of inflammatory factors in colon tissue according to the instructions of the kit. Fig.10 As shown, it can be seen that compared with the Control group, the TNF-α, IL-10 and IL-6 in the DSS group were significantly higher (P < 0.01) than those in the Control group, and the IL-12 in the DSS group was significantly higher (P < 0.05) than those in the Control group; compared with the DSS group, the IL-6 in the JK-23+DSS group was significantly lower (P < 0.01) than that in the DSS modeling group, and the IL-10, IL-12 and TNF-α in the JK-23+DSS group were significantly lower (P < 0.05) than those in the DSS modeling group.

[0068] The Acetobacter Ghana JK-23 provided by the present invention can reduce the levels of inflammatory factors in serum and colon tissue, which helps Acetobacter Ghana JK-23 to play a key role in the prevention and / or treatment of inflammatory bowel disease and other fields.

[0069] 5. Detection of oxidative stress factors in mouse serum

[0070] Blood samples were collected from mice in each treatment group after enucleation in step 2, and the samples were centrifuged at 4000 rpm for 10 min to obtain serum. Serum was aspirated and stored at -80°C for later use. The levels of catalase (CAT), myeloperoxidase (MPO), superoxide dismutase (SOD) and malondialdehyde (MDA) in mouse serum were detected using a biochemical kit, and the levels of inflammatory factors in serum were determined according to the kit instructions. Fig.11As shown, it can be seen that compared with the Control group, the SOD and CAT of the DSS group were extremely significantly lower (P < 0.01) than those of the Control group, and the MPO and MDA of the DSS group were extremely significantly higher (P < 0.01) than those of the Control group; compared with the DSS group, the CAT of the JK-23+DSS group was extremely significantly higher (P < 0.01) than that of the DSS modeling group, the SOD of the JK-23+DSS group was significantly lower (P < 0.05) than that of the DSS modeling group, and the MPO and MDA of the JK-23+DSS group were significantly higher (P < 0.05) than those of the DSS modeling group.

[0071] In summary, the Acetobacter ghanensis strain of the present invention has strong antioxidant activity, can effectively relieve intestinal oxidative stress and colitis, can be used for intestinal mucosal damage, intestinal atrophy, and bloody stools, and can provide a strain source for the development and application of natural antioxidants and anti-inflammatory ingredients for humans or animals, such as in feed and food processing.

Claims

1. A strain of Acetobacter ghana JK-23 was deposited in the China Center for Type Culture Collection on July 3, 2024, with the deposit address being Wuhan University, China, and the deposit number being CCTCC NO: M 20241457.

2. Use of Acetobacter Ghana JK-23 according to claim 1 in the preparation of medicines, reagents, functional foods, special medical formula foods or health products for treating or alleviating cell oxidative damage, intestinal mucosal damage, intestinal atrophy, blood in stool and colitis.

3. Use of Acetobacter ghana JK-23 according to claim 1 in the preparation of drugs, reagents, functional foods, special medical purpose formula foods or health products for treating or alleviating H2O2-induced oxidative damage to IPEC-J2 cells.

4. Use of the Acetobacter Ghana JK-23 according to claim 1 in the preparation of drugs, reagents, functional foods, special medical purpose formula foods or health products for treating or alleviating DSS-induced colitis.

5. The application according to claim 4, characterized in that: The Acetobacter Ghana JK-23 treats or alleviates DSS-induced colitis by changing the length of the colon and the structure of the colon tissue, while reducing the levels of inflammatory factors in serum and colon tissue and increasing the levels of anti-oxidative stress factors.

6. A bacterial agent produced by using the Acetobacter ghana JK-23 of claim 1 for treating or alleviating cell oxidative damage, intestinal mucosal damage, intestinal atrophy, bloody stool and colitis.

7. The bacterial agent according to claim 6, characterized in that The anti-inflammatory bacterial agent is used for treating and alleviating H2O2-induced IPEC-J2 cell oxidative damage and DSS-induced colitis.

8. The bacterial agent according to claim 6, characterized in that The bacterial agent contains the Acetobacter ghana JK-23 and / or the culture of Acetobacter ghana JK-23.

9. The bacterial agent according to claim 6, characterized in that The culture is a bacterial suspension obtained by culturing the Acetobacter Ghana JK-23 and then adding physiological saline to resuspend the bacterial cells, or a cell-free extract of Acetobacter Ghana JK-23.

10. The bacterial agent according to claim 6, characterized in that The content of Acetobacter Ghana JK-23 in the anti-inflammatory bacterial agent is not less than 1×10 9 CFU / mL.

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