Process for detecting carbon content of strain domestication tank

By introducing heating and indicator mechanisms into the bacterial culture acclimation device, decomposing and detecting inorganic carbon, the TOC detection problem caused by excessive inorganic carbon in the prior art is solved, and the working efficiency and measurement quality are improved.

CN119492861APending Publication Date: 2025-02-21CHINA PETROLEUM & CHEMICAL CORP +1
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Patent Information

Application Number
CN202311012833.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2023-08-12
Publication Date
2025-02-21

AI Technical Summary

Technical Problem

In the existing bacterial culture acclimation device, the inorganic carbon content of the effluent water is high, resulting in the number of TOC detection being 0, and the testing equipment needs to be replaced, which increases the labor burden of analysts.

Method used

A carbon content detection process for bacterial culture acclimation tanks was designed. Using the heating mechanism and indicator mechanism in the bacterial culture acclimation device, the inorganic carbon is decomposed into carbon dioxide by heating the liquid and adding hydrochloric acid solution. The threshold value of carbon dioxide gas is detected by using the indicator mechanism to achieve Inspection of inorganic carbon content.

Benefits of technology

It effectively avoids the problem that the TOC tests organic carbon number 0 when detecting organic carbon caused by excessive inorganic carbon, reduces the need to replace the detection equipment, improves work efficiency, and ensures the quality of measurement.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention discloses a strain domestication tank carbon content detection process, and belongs to the technical field of strain domestication. Quantitative liquid in the domestication tank (1) enters the heating mechanism (6); the liquid adding mechanism (10) adds an acid solution into the heating mechanism (6) until the switching mechanism (11) closes the liquid adding mechanism (10); the heating mechanism (6) is used for heating the mixed liquid; the instruction means (7) detects the generated carbon dioxide gas and instructs whether or not the carbon dioxide gas exceeds a threshold value. The process for detecting the carbon content of the strain domestication tank realizes the detection of the content of inorganic carbon in the liquid, prevents the situation that the reading is 0 during TOC detection of organic carbon and detection equipment needs to be replaced due to excessive inorganic carbon content during direct detection, relieves the burden of personnel, and improves the working efficiency.
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Description

Technical Field

[0001] The invention discloses a carbon content detection process for a strain domestication tank, belonging to the technical field of strain domestication. Background Art

[0002] Bacterial strains refer to the propagation materials composed of mycelium and growth matrix of edible fungi, industrial fungi, and agricultural fungi. Bacterial strain domestication generally refers to a method of selectively breeding microorganisms by gradually adapting microorganisms to certain conditions through artificial measures. Through domestication, strains with higher tolerance and activity can be obtained. Domestication is often used in the breeding of microorganisms in wastewater treatment to obtain efficient strains with higher degradation ability for certain pollutants. At present, bacterial strain domestication is usually carried out in a domestication device, such as a BOD bacterial strain continuous culture domestication device disclosed in utility model CN ​​218146069 U.

[0003] The current strain domestication device has a high content of inorganic carbon in the domesticated water. When the combustion method is used to detect the TOC (total organic carbon) of the water, the TOC detection reading is 0, and it is necessary to replace NPOC (non-purgeable organic carbon) for detection, which brings unnecessary labor burden to analysts. For this reason, we propose a strain domestication device to solve the above problem.

[0004] Chinese invention patent application CN105716927A discloses a new device and method for removing inorganic carbon and a device and method for detecting total organic carbon. The device for removing inorganic carbon in the invention includes two spaces, the first space is used to receive a first water flow containing a first concentration of inorganic carbon and provide carbon dioxide converted from the inorganic carbon and an analysis flow containing a second concentration of inorganic carbon, and the second space is used to receive a second water flow and absorb the carbon dioxide from the first space. The purpose of the invention is to remove carbon dioxide from the sample, eliminate the influence of volatile organic matter being purged and drawn away, and fully reflect the total organic carbon content in the water.

[0005] Chinese invention patent CN111912933B discloses a method and system for measuring total organic carbon in water. The method provided by the invention is to acidify the sample water, that is, convert the inorganic carbon in the sample into carbon dioxide, and then remove the carbon dioxide in the sample by aeration; add the sample with inorganic carbon removed to a high-temperature combustion tube, and then measure it by non-dispersive infrared absorption method to obtain a concentration value a; at the same time, pass the aeration gas into a gas collection vessel, and use ultraviolet spectroscopy to detect the volatile organic matter in the vessel, and the measured concentration is recorded as b, and the total organic carbon (TOC) concentration in water is a+b. The purpose of this invention is to use a method to measure total organic carbon in water, so that the measurement accuracy is higher than that of conventional methods, and the maintenance cost is low. Summary of the invention

[0006] The technical problem to be solved by the present invention is to overcome the shortcomings of the prior art and provide a carbon content detection process for a strain acclimation tank, which can avoid the TOC reading being 0 when detecting organic carbon due to excessive inorganic carbon.

[0007] The technical solution adopted by the present invention to solve the technical problem is: the carbon content detection process of the strain acclimation tank is characterized by: adopting a strain acclimation device to achieve the detection, the strain acclimation device comprises an acclimation tank, a heating mechanism, an indicating mechanism, a liquid adding mechanism and a switch mechanism, the heating mechanism is connected to the bottom of the acclimation tank, and a main water valve is arranged between the heating mechanism and the acclimation tank, the indicating mechanism is connected to the top of the heating mechanism, the liquid adding mechanism is connected to the upper part of the heating mechanism, and the switch mechanism is arranged between the liquid adding mechanism and the heating mechanism; The detection process includes the following steps: S1 Add a certain amount of liquid in the acclimation tank into the heating mechanism; The S2 solution pushes the liquid adding mechanism to move and adds the acid solution into the heating mechanism; S3 heating mechanism heats the mixed liquid; S4 indicates that the indicator detects the generated carbon dioxide gas and indicates whether the carbon dioxide gas exceeds a threshold value.

[0008] Preferably, the mixed solution in step S3 is heated to 150°C.

[0009] Preferably, the acid solution in step S2 is a hydrochloric acid solution.

[0010] Preferably, the heating mechanism includes a heating tube, a drain pipe and a heating ring. The heating tube is vertically arranged, the top of the heating tube is connected to the acclimation tank, the bottom of the heating tube is closed, the drain pipe is arranged on the lower side of the heating tube and is connected to the heating tube, a sealing cover is detachably installed on the bottom of the drain pipe, and the heating ring is sleeved on the outside of the heating tube.

[0011] Preferably, the heating mechanism further comprises a protective cover, which is sleeved outside the heating tube, and the heating ring is arranged between the liquid inlet tube and the protective cover.

[0012] Preferably, the indicating mechanism comprises an indicating tube, a push plate and an indicating rod. The upper end of the indicating tube is closed, and the lower end is sealed to the heating mechanism. An air inlet connected to the indicating tube is provided on the heating mechanism. The push plate is slidably arranged in the indicating tube, and the push plate is sealed to the inner wall of the indicating tube. The lower end of the indicating rod can slide through the closed end of the indicating tube and then be connected to the push plate.

[0013] Preferably, the strain domestication device also includes a faucet, which is connected to the lower part of the domestication tank, the main water valve is arranged at one end of the faucet close to the domestication tank, the heating mechanism is arranged on the lower side of the faucet, and the indicating mechanism is arranged on the upper side of the faucet.

[0014] Preferably, a small water valve is provided at one end of the faucet away from the acclimation tank.

[0015] Compared with the prior art, the present invention has the following beneficial effects: 1. When the carbon content detection process of the strain acclimation tank is being tested, the liquid inside the acclimation tank enters the heating mechanism, and the liquid flow drives the liquid adding mechanism to work, so that the hydrochloric acid solution in the reagent tube enters the heating mechanism. After the liquid reaches a certain amount, the heating mechanism is started to heat, so that the inorganic carbon in the liquid is decomposed into carbon dioxide gas under the action of hydrochloric acid and enters the indicating mechanism. By setting a threshold on the indicating mechanism, the inorganic carbon content in the liquid is checked to prevent the excessive inorganic carbon content during direct inspection from causing the TOC to show 0 when detecting organic carbon, and the need to replace the detection equipment, thereby reducing the burden on personnel and improving work efficiency.

[0016] 2. During the test, the liquid flow drives the liquid adding mechanism to make the hydrochloric acid solution in the reagent tube enter the heating mechanism. The continuous increase of liquid in the heating mechanism causes the switch mechanism to move upward under the buoyancy of the liquid. The switch mechanism is closed, and the liquid cannot enter the heating mechanism, thus realizing the quantitative addition of hydrochloric acid solution and the quantitative addition of liquid in the acclimation tank, preventing the excessive addition of liquid and hydrochloric acid solution from affecting the measurement accuracy, and ensuring the measurement quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a three-dimensional schematic diagram of a bacterial strain domestication device; Figure 2 It is a three-dimensional schematic diagram of the bacterial strain domestication device after being cut open; Figure 3 It is a three-dimensional schematic diagram of the cutaway heating tube and the reagent tube; Figure 4 It is a three-dimensional schematic diagram of the switch mechanism and the liquid adding mechanism; Figure 5 A three-dimensional schematic diagram of the switch mechanism and the liquid adding mechanism from another perspective; Figure 6 for Figure 2 A partial enlarged view of point A in the middle.

[0018] In the figure: 1. acclimatization tank; 2. control panel; 3. connecting pipe; 4. faucet; 5. main water valve; 6. heating mechanism; 61. heating tube; 62. drain pipe; 63. heating ring; 64. protective cover; 7. indication mechanism; 71. indication tube; 72. air inlet; 73. push plate; 74. indication rod; 8. small water valve; 9. medicine tube; 10. liquid adding mechanism; 101. impeller; 102. rotating shaft; 103. liquid adding wheel; 104. liquid inlet hole; 11. switch mechanism; 111. buoyancy ring; 112. pull rope; 113. sealing plate; 114. reset spring; 115. fixing plate; 116. exhaust hole; 117. liquid inlet tank. DETAILED DESCRIPTION

[0019] The present invention is further described below in conjunction with specific embodiments. However, people familiar with the art should understand that the detailed description given here in conjunction with the drawings is for better explanation, and the structure of the present invention necessarily exceeds these limited embodiments. For some equivalent replacement schemes or common means, they are no longer described in detail herein, but still belong to the scope of protection of the present application.

[0020] Figures 1 to 6 The best embodiment of the present invention is shown below in conjunction with the attached Figures 1 to 6 The present invention is further described.

[0021] The strain acclimation device includes an acclimation tank 1, a heating mechanism 6, an indicating mechanism 7, a liquid adding mechanism 10 and a switch mechanism 11. The heating mechanism 6 is connected to the bottom of the acclimation tank 1, and a main water valve 5 is arranged between the heating mechanism 6 and the acclimation tank 1. The indicating mechanism 7 is connected to the top of the heating mechanism 6, the liquid adding mechanism 10 is connected to the upper part of the heating mechanism 6, and the switch mechanism 11 is arranged between the liquid adding mechanism 10 and the heating mechanism 6. When the carbon content detection process of the strain acclimation tank is being tested, the liquid inside the acclimation tank 1 enters the heating mechanism 6, and the liquid flow drives the liquid adding mechanism 10 to work, so that the hydrochloric acid solution in the reagent tube 9 enters the heating mechanism 6. After the liquid reaches a certain amount, the heating mechanism 6 is started to heat, so that the inorganic carbon in the liquid is decomposed into carbon dioxide gas under the action of hydrochloric acid and enters the indicating mechanism 7. By setting a threshold value on the indicating mechanism 7, the inorganic carbon content in the liquid is checked to prevent the excessive inorganic carbon content during direct inspection from resulting in the TOC organic carbon reading of 0, which requires the replacement of the detection equipment. This reduces the burden on personnel and improves work efficiency.

[0022] Specific: Refer to the attached Figure 1-6A control panel 2 is installed on the outer wall of the acclimation tank 1, a connecting pipe 3 is installed on the outer wall of the acclimation tank 1, a faucet 4 is installed on the outer wall of the acclimation tank 1, a heating mechanism 6 is installed at the bottom of the faucet 4, and the heating mechanism 6 is used to heat the liquid discharged from the acclimation tank 1. An indicating mechanism 7 is installed on the top of the faucet 4, and the indicating mechanism 7 is used to indicate the amount of carbon dioxide produced by decomposition, and a threshold marking line is set on the outer wall of the indicating mechanism 7. A liquid adding mechanism 10 is installed in the heating mechanism 6, and the liquid adding mechanism 10 is used to quantitatively add hydrochloric acid solution. A switch mechanism 11 is installed on the top of the heating mechanism 6, and the switch mechanism 11 is used to quantitatively add liquid entering the heating mechanism 6.

[0023] By setting the above structure, the content of inorganic carbon in the liquid can be checked to prevent excessive inorganic carbon content during direct inspection, resulting in the TOC reading being 0 when detecting organic carbon, and the need to replace the detection equipment, thereby reducing the burden on personnel and improving work efficiency.

[0024] A main water valve 5 is installed on the side of the faucet 4 close to the acclimation tank 1 , a small water valve 8 is installed on the side of the faucet 4 away from the acclimation tank 1 , and a medicine tube 9 is fixedly connected to the outer side wall of the heating mechanism 6 .

[0025] The heating mechanism 6 includes a heating tube 61, a drain pipe 62, a heating ring 63 and a protective cover 64. The heating tube 61 is a vertically arranged circular tube. The bottom of the heating tube 61 is fixedly connected to the top of the drain pipe 62. The bottom of the heating tube 61 is closed. The diameter of the drain pipe 62 is smaller than the diameter of the heating tube 61, and the drain pipe 62 is coaxially arranged on the lower side of the heating tube 61. The top of the drain pipe 62 is connected to the bottom of the heating tube 61. The lower end of the drain pipe 62 is detachably installed with a sealing cover to close the bottom of the drain pipe 62. The protective cover 64 is sleeved outside the heating tube 61, the outer side wall of the drain tube 62 is fixedly connected to the bottom of the protective cover 64, the heating ring 63 is arranged between the protective cover 64 and the heating tube 61, the inner ring of the heating ring 63 is fixedly connected to the outer side wall of the heating tube 61, the inner wall of the protective cover 64 is fixedly connected to the outer ring of the heating ring 63, the top of the protective cover 64 is bent inwardly and fixedly connected to the outer wall of the heating tube 61, and the top of the heating tube 61 is fixedly connected to the bottom of the faucet 4; By setting the above structure, the liquid and the hydrochloric acid solution are heated, and the decomposition rate of the inorganic carbon in the liquid is guaranteed.

[0026] The indicating mechanism 7 comprises an indicating tube 71, an air inlet 72, a push plate 73 and an indicating rod 74. The indicating tube 71 is a vertically arranged circular tube, and the top of the indicating tube 71 is closed. The bottom of the indicating tube 71 is fixedly connected to the top of the faucet 4, and the indicating tube 71 is arranged opposite to the top of the heating tube 61. The faucet 4 is provided with an air inlet 72 opposite to the indicating tube 71, and the air inlet 72 connects the faucet 4 with the indicating tube 71. The air inlet 72 is provided at the middle position of the bottom of the indicating tube 71, and the push plate 73 is arranged in the indicating tube 71, and the outer wall of the push plate 73 is slidably connected to the inner wall of the indicating tube 71, and the push plate 73 and the indicating tube 71 are sealed. The bottom of the indicating rod 74 is fixedly connected to the top of the push plate 73, and the indicating rod 74 passes through the closed end of the indicating tube 71 and is slidably connected to the indicating tube 71.

[0027] Through the arrangement of the above structure, the inorganic carbon content in the liquid is effectively detected, which makes it convenient for personnel to directly understand the inorganic carbon content in the liquid.

[0028] The liquid adding mechanism 10 includes an impeller 101, a rotating shaft 102, a liquid adding wheel 103 and a liquid inlet hole 104; the impeller 101 is coaxially mounted on the rotating shaft 102, and one end of the rotating shaft 102 away from the impeller 101 is coaxially connected to the liquid adding wheel 103, and the liquid inlet hole 104 is opened on the outer wall of the liquid adding wheel 103, and the liquid inlet hole 104 is a blind hole arranged along the radial direction of the liquid adding wheel 103, and a plurality of liquid inlet holes 104 are evenly spaced around the rotating shaft 102.

[0029] The liquid adding mechanism 10 also includes a liquid inlet pipe, which is horizontally arranged, and both ends of the liquid inlet pipe are open, one end of the liquid inlet pipe is connected to the top of the heating tube 61, and the other end is connected to the vertical medicine tube 9. The top of the medicine tube 9 is closed, and the top of the medicine tube 9 is provided with a liquid adding hole.

[0030] The rotating shaft 102 and the liquid adding wheel 103 are both arranged in the liquid adding pipe, and the liquid adding wheel 103 is arranged directly below the medicine pipe 9, and the medicine pipe 9 can be connected with each liquid inlet hole 104. The top of the liquid adding wheel 103 is sealed with the inner wall of the liquid adding pipe, and a gap is arranged between the liquid adding wheel 103 and the inner wall of the liquid adding pipe. The impeller 101 is arranged in the upper part of the heating pipe 61.

[0031] When the solution in the acclimation tank 1 enters the heating tube 61, the solution will drive the impeller 101 to rotate, and the impeller 101 drives the liquid adding wheel 103 to rotate through the rotating shaft 102, so that the hydrochloric acid solution in the medicine tube 9 gradually enters the liquid adding pipe, and enters the heating tube 61 through the liquid adding pipe. When the liquid inlet hole 104 is facing upward and facing the medicine tube 9, the hydrochloric acid solution in the medicine tube 9 enters the liquid inlet hole 104 on the upper side. When the liquid inlet hole 104 rotates to the lower side of the liquid adding wheel 103, the hydrochloric acid solution in the liquid inlet hole 104 enters the liquid adding pipe. In order to ensure that the hydrochloric acid solution in the liquid adding pipe can flow smoothly into the heating tube 61, the liquid adding pipe can be inclined gradually downward in the direction close to the heating tube 61.

[0032] Through the arrangement of the above structure, quantitative addition of the hydrochloric acid solution according to the liquid content is achieved, stable addition of the hydrochloric acid solution is ensured, and waste caused by excessive addition is prevented.

[0033] The switch mechanism 11 includes a buoyancy ring 111, a pull rope 112, a sealing plate 113, a reset spring 114 and a fixed plate 115; the buoyancy ring 111 is arranged at intervals on the lower side of the fixed plate 115, and the buoyancy ring 111 and the fixed plate 115 are arranged coaxially. The fixed plate 115 is arranged on the top of the heating tube 61 and closes the top of the heating tube 61, and the buoyancy ring 111 is slidably installed on the heating tube 61. A liquid inlet groove 117 and an exhaust hole 116 are arranged on the fixed plate 115, and the exhaust hole 116 is arranged in the middle of the fixed plate 115, and the exhaust hole 116 is opposite to the air inlet 72. There are a number of liquid inlet grooves 117 evenly spaced around the fixed plate 115. In the present embodiment, there are four liquid inlet grooves 117 evenly spaced around the fixed plate 115. The fixed plate 115 is provided with a sealing plate 113, which is slidably connected to the fixed plate 115, and the sealing plate 113 corresponds to the liquid inlet groove 117 one by one, and closes the pushed liquid inlet groove 117. A return spring 114 is provided between the outer side of each sealing plate 113 and the fixed plate 115, and the return spring 114 is in compression filling, one end of the return spring 114 is connected to the fixed plate 115, and the other end is connected to the sealing plate 113, and pushes the sealing plate 113 to move in the direction close to the middle of the fixed plate 115 to open the corresponding liquid inlet groove 117. In this embodiment, two return springs 114 are symmetrically provided at the outer end of each sealing plate 113. The pull rope 112 is arranged between the fixed plate 115 and the buoyancy ring 111, and the lower end of the pull rope 112 is fixedly connected to the buoyancy ring 111, and the upper end can slide through the fixed plate 115 and then be connected to the outer end of the sealing plate 113. The pull rope 112 corresponds to the sealing plate 113. The impeller 101 is disposed between the fixing plate 115 and the buoyancy ring 111. To prevent the pull rope 112 from being wound around the impeller 101, the pull rope 112 is slidably connected to the inner wall of the heating tube 61.

[0034] A guide rod is fixedly connected to the outer side of the sealing plate 113 . The guide rod is located between the two return springs 114 . The guide rod is slidably connected to the fixed plate 115 .

[0035] By setting the above structure, the volume of liquid entering the heating mechanism 6 is effectively controlled, preventing the addition of too much liquid and hydrochloric acid solution from affecting the measurement accuracy, thereby ensuring the quality of the measurement.

[0036] A one-way valve is provided in the middle of the buoyancy ring 111 so that the solution can only flow downward through the buoyancy ring 111 but cannot flow upward through the buoyancy ring 111, thereby ensuring that the solution in the heating tube 61 can move the buoyancy ring 111 upward.

[0037] A carbon content detection process for a strain acclimation tank comprises the following steps: S1: adding a certain amount of liquid in the acclimation tank 1 into the heating mechanism 6.

[0038] Open the main water valve 5 and close the small water valve 8. At this time, the liquid in the acclimation tank 1 enters the heating tube 61 through the faucet 4 and the liquid inlet groove 117.

[0039] The S2 solution pushes the liquid adding mechanism 10 to move forward, and the acid solution is added into the heating mechanism 6.

[0040] The flow of liquid in the heating tube 61 drives the impeller 101 to rotate, and then drives the rotating shaft 102 to rotate, so that the liquid adding wheel 103 rotates, and the hydrochloric acid solution in the medicine tube 9 enters the heating tube 61 through the liquid inlet hole 104. The continuous increase of liquid in the heating tube 61 causes the buoyancy ring 111 to move upward under the action of buoyancy, so that the pull rope 112 is loosened. At this time, the sealing plate 113 seals the liquid inlet groove 117 under the action of the reset spring 114, and then closes the main water valve 5.

[0041] S3 The heating mechanism 6 heats the mixed liquid.

[0042] The heating ring 63 is started to heat the solution in the heating tube 61 until it reaches 150 degrees Celsius, and then the heating can be stopped.

[0043] S4: the indicating mechanism 7 detects the generated carbon dioxide gas and indicates whether the carbon dioxide gas exceeds a threshold value.

[0044] The inorganic carbon in the liquid decomposes to generate carbon dioxide under the action of hydrochloric acid. The carbon dioxide gas will enter the indicator tube 71 through the exhaust hole 116 and the air inlet 72, pushing the push plate 73 to drive the indicator rod 74 to move upward. When the indicator rod 74 is no longer moving, it can penetrate the indicator tube 71 to see whether the carbon dioxide content has reached the threshold value. Then, according to the test results, a suitable detection equipment is selected to detect the content of total organic carbon. The main water valve 5 and the small water valve 8 can be opened at the same time to allow the liquid in the acclimation tank 1 to be discharged through the faucet 4. After the discharge reaches a certain amount, the main water valve 5 and the small water valve 8 are closed at the same time, and the drain pipe 62 is opened to completely discharge the liquid in the heating tube 61 and then the drain pipe 62 is closed to complete the entire operation. The operation is simple and quick, and it is convenient for personnel to use.

[0045] The above is only a preferred embodiment of the present invention, and does not limit the present invention in other forms. Any technician familiar with the profession may use the above disclosed technical content to change or modify it into an equivalent embodiment with equivalent changes. However, any simple modification, equivalent change and modification made to the above embodiment according to the technical essence of the present invention without departing from the technical solution of the present invention still belongs to the protection scope of the technical solution of the present invention.

Claims

1. A carbon content detection process for a strain acclimation tank, characterized in that: The method is implemented by using a strain domestication device, which comprises a domestication tank (1), a heating mechanism (6), an indicating mechanism (7), a liquid adding mechanism (10), and a switch mechanism (11); the heating mechanism (6) is connected to the bottom of the domestication tank (1), and a main water valve (5) is arranged between the heating mechanism (6) and the domestication tank (1); the indicating mechanism (7) is connected to the top of the heating mechanism (6), the liquid adding mechanism (10) is connected to the upper part of the heating mechanism (6), and the switch mechanism (11) is arranged between the liquid adding mechanism (10) and the heating mechanism (6); The detection process includes the following steps: S1: adding a certain amount of liquid in the acclimation tank (1) into the heating mechanism (6); The S2 solution drives the liquid adding mechanism (10) to move and adds the acid solution into the heating mechanism (6); S3 heating mechanism (6) heats the mixed liquid; S4 indicating mechanism (7) detects the generated carbon dioxide gas and indicates whether the carbon dioxide gas exceeds a threshold value.

2. The carbon content detection process of the strain acclimation tank according to claim 1 is characterized in that: The mixed solution in step S3 is heated to 150°C.

3. The carbon content detection process of the strain acclimation tank according to claim 1 is characterized in that: The acid solution in step S2 is a hydrochloric acid solution.

4. The carbon content detection process of the strain acclimation tank according to claim 1 is characterized in that: The heating mechanism (6) comprises a heating tube (61), a drainage tube (62) and a heating ring (63); the heating tube (61) is arranged vertically; the top of the heating tube (61) is connected to the acclimation tank (1); the bottom of the heating tube (61) is arranged in a closed manner; the drainage tube (62) is arranged at the lower side of the heating tube (61) and is connected to the heating tube (61); a sealing cover is detachably installed at the bottom of the drainage tube (62); and the heating ring (63) is sleeved on the outside of the heating tube (61).

5. The carbon content detection process of the strain acclimation tank according to claim 4 is characterized in that: The heating mechanism (6) further comprises a protective cover (64), the protective cover (64) being sleeved outside the heating tube (61), and the heating ring (63) being arranged between the liquid inlet tube (61) and the protective cover (64).

6. The carbon content detection process of the strain acclimation tank according to claim 1 is characterized in that: The indicating mechanism (7) comprises an indicating tube (71), a push plate (73) and an indicating rod (74); the upper end of the indicating tube (71) is closed, and the lower end is sealedly connected to the heating mechanism (6); an air inlet (72) communicating with the indicating tube (71) is provided on the heating mechanism (6); the push plate (73) is slidably arranged in the indicating tube (71), and the push plate (73) and the inner wall of the indicating tube (71) are sealed; the lower end of the indicating rod (74) is slidably passed through the closed end of the indicating tube (71) and is connected to the push plate (73).

7. The carbon content detection process of the strain acclimation tank according to claim 1 is characterized in that: The bacterial strain domestication device further comprises a faucet (4), the faucet (4) being connected to the lower part of the domestication tank (1), a main water valve (5) being arranged at one end of the faucet (4) close to the domestication tank (1), a heating mechanism (6) being arranged at the lower side of the faucet (4), and an indicating mechanism (7) being arranged at the upper side of the faucet (4).

8. The carbon content detection process of the strain acclimatization tank according to claim 7 is characterized in that: A small water valve (8) is provided at one end of the faucet (4) away from the acclimation tank (1).

Citation Information

Patent Citations

  • Facility and method for removing inorganic carbon, and apparatus and method for detecting total organic carbon

    CN105716927A

  • A method and system for measuring total organic carbon in water

    CN111912933B