Traditional Chinese medicine composition for rapidly relieving renal colic and preparation method thereof

Abstract

The invention relates to the technical field of traditional Chinese medicine composition production, in particular to a traditional Chinese medicine composition for rapidly relieving renal colic and a preparation method thereof. Comprising the following components in parts by weight: 10-30 parts of lysimachia christinae hance nanoparticles, 6-16 parts of rhizoma corydalis, 8-18 parts of radix paeoniae alba, 5-15 parts of endothelium corneum gigeriae galli, 8-18 parts of rhizoma alismatis, 5-10 parts of lygodium japonicum, 2-8 parts of beta-cyclodextrin, 0.5-1.5 parts of a poloxamer surfactant and 2-8 parts of sodium carboxymethyl cellulose. According to the invention, flavonoid glycoside in lysimachia christinae hance is extracted, glycosylated and emulsified into nano-particles, so that the absorption and utilization rate of flavonoid glycoside in a body are improved, and the drug effect of flavonoid glycoside is improved. Beta-cyclodextrin and sodium carboxymethyl cellulose are adopted as carriers, the release speed of the traditional Chinese medicine composition can be regulated and controlled, the release speed of components is slowed down to a certain degree, and the medicine can continuously play a role in the body.

Description

Technical Field

[0001] The invention relates to the technical field of production of traditional Chinese medicine compositions, in particular to a traditional Chinese medicine composition for rapidly relieving renal colic and a preparation method thereof. Background Art

[0002] Renal colic is a common and painful urinary system disease, mainly caused by kidney stones, and manifested as severe pain in the waist or abdomen. This pain often occurs suddenly and lasts for a long time, causing great discomfort and pain to the patient. First, when renal colic occurs, patients often need emergency medical treatment, but the waiting time and treatment process in the hospital may prolong the patient's suffering time. A fast and effective Chinese medicine composition can be used immediately at home or at the first aid site to quickly relieve pain and reduce the patient's pain and medical treatment time. Secondly, the safety and effectiveness of the Chinese medicine composition have been widely recognized. Traditional Chinese medicine has a long history and rich clinical application experience, and many traditional Chinese medicines have been proven to have significant therapeutic effects on renal colic.

[0003] Traditional treatment methods mainly include drug therapy, physical therapy and surgical treatment, but these methods often take a long time to be effective and may be accompanied by significant side effects. Some Chinese medicine ingredients have low solubility in water, resulting in slow absorption of the drug in the body and low bioavailability. Chinese medicine compositions may undergo physical changes such as agglomeration and stratification during storage, affecting the uniformity and stability of the drug, resulting in reduced efficacy or failure. In view of this, we propose a Chinese medicine composition for quickly relieving renal colic and a preparation method thereof. Summary of the invention

[0004] The object of the present invention is to provide a Chinese medicine composition for rapidly relieving renal colic and a preparation method thereof, so as to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above object, on the one hand, the present invention provides a Chinese medicine composition for rapidly relieving renal colic, comprising the following components: 10-30 parts by weight of Lysimachia chinensis nanoparticles, 6-16 parts by weight of Corydalis yanhusuo, 8-18 parts by weight of Paeonia lactiflora, 5-15 parts by weight of Gallus gallus domestica, 8-18 parts by weight of Rhizoma alismatis, 5-10 parts by weight of Lygodium japonicum, 2-8 parts by weight of β-cyclodextrin, 0.5-1.5 parts by weight of poloxamer surfactant, and 2-8 parts by weight of sodium carboxymethyl cellulose; The Lysimachia nanoparticles are prepared by glycosylation reaction of flavonoid glycosides in the Lysimachia with sugar, followed by emulsification into nanoparticles; the sugar is any one of glucose, galactose and arabinose.

[0006] Preferably, the preparation method of the Lysimachia sibiricum nanoparticles is as follows: S1.1, weigh the following components in parts by weight respectively: 6-15 parts by weight of Lysimachia sibiricum, 40-60 parts by weight of ethanol, 3-8 parts by weight of sugar, 0.5-2 parts by weight of citric acid catalyst, 20-40 parts by weight of ethyl acetate solvent, and 5-15 parts by weight of polyvinyl alcohol surfactant; S1.2, crush the Lysimachia chinensis with a grinder at a speed of 1000-3000 rpm for 3-5 min to obtain Lysimachia chinensis powder with a particle size of 80-120 mesh; The desmodium powder is dissolved in 50-70% ethanol, and heated under reflux at 60-80° C. for 2-3 hours to obtain an extract, and the extract is cooled to room temperature and filtered to obtain a filtrate; The filtrate is placed in a rotary evaporator for concentration to obtain flavonoid glycosides; Lysimachia is a complex natural plant, containing a variety of ingredients, including flavonoids, alkaloids, volatile oils, etc. If Lysimachia is used directly, its ingredients are numerous and complex. By extracting flavonoid glycosides and performing glycosylation, the water solubility of flavonoid glycosides can be increased, and its absorption and utilization efficiency in the body can be improved, thereby improving its efficacy. At the same time, the glycosylated products may be more stable in chemical structure than the original flavonoid glycosides, and are not easily damaged by factors such as light, heat, and oxidation, which is conducive to long-term storage and use. If Lysimachia is used directly, the flavonoid glycosides inside it may be degraded during storage due to its own physiological changes or external environmental factors, affecting its function. Other ingredients (alkaloids) in Centella asiatica may cause potential toxicity or side effects; by extracting flavonoid glycosides, the interference of other potentially harmful ingredients can be reduced to a certain extent, and the biosafety of glycosylated flavonoid glycosides may also be improved after structural modification, reducing the risk of adverse reactions during use.

[0007] S1.3, dissolving flavonoid glycosides and sugar in deionized water, stirring with a magnetic stirrer at 300-500 rpm for 15-30 min at 30-40°C to obtain a mixed solution; The citric acid catalyst is added to the mixed solution and stirred continuously at a speed of 200-300 rpm for 10-30 min. The glycosylation reaction is carried out at 50-60° C. for 6-24 h. S1.4. After the reaction is completed, the reaction mixture is filtered; the filtrate is placed in a rotary evaporator at a temperature of 50-60°C and a rotation speed of 50-100 rpm to obtain flavonoid glycosides; Dissolve the flavonoid glycoside in ethyl acetate and the polyvinyl alcohol surfactant in water; mix the two solutions and stir vigorously, place in a fume hood, and form a nanoparticle suspension with a particle size of 75-120 μm; After glycosylation, the polarity of flavonoid glycosides increases, which improves their solubility and stability in aqueous media, and is beneficial for their application in water-based preparations (such as oral solutions, tea drinks, etc.), making them easier to be absorbed by the human body. Glycosylation can give flavonoid glycosides specific targeting, making it easier for them to reach specific tissues or organs; in addition, flavonoid glycosides can better resist degradation by gastric acid, promote their absorption in the intestine, and thus improve bioavailability; in terms of taste, glycosylation helps to reduce the bitterness and astringency of flavonoid glycosides, expands their application range in the food and beverage industry, and enhances consumer experience.

[0008] S1.5, centrifuging the nanoparticle suspension at a rotation speed of 1000-2000 rpm for 10-20 min, washing the nanoparticles with deionized water; vacuum drying the nanoparticles to obtain Lysimachia sibiricum nanoparticles.

[0009] The encapsulation function of nanoparticles can effectively isolate flavonoid glycosides from oxygen, moisture and light in the external environment, thereby reducing their degradation risk; the use of surfactants such as polyvinyl alcohol can further enhance the stability of nanoparticles, avoid their aggregation and precipitation, and ensure the quality of the preparation; the small size and high specific surface area of ​​nanoparticles mean that flavonoid glycosides can contact target molecules more frequently, thereby enhancing their biological activity and improving efficacy.

[0010] Preferably, the temperature of the rotary evaporator is 50-70°C and the rotation speed is 50-100 rpm.

[0011] Preferably, in S1.4, the vigorous stirring is at a stirring speed of 800-1000 rpm at room temperature for 10-20 min.

[0012] Preferably, the vacuum drying temperature is set at 30-50°C, and the drying time is 12-24h.

[0013] On the other hand, the present invention provides a method for preparing a Chinese medicine composition for rapidly relieving renal colic, which is used to prepare the Chinese medicine composition for rapidly relieving renal colic, comprising the following steps: S1. Weigh the following components in parts by weight respectively: 10-30 parts by weight of Lysimachia cyrtonema nanoparticles, 6-16 parts by weight of Corydalis yanhusuo, 8-18 parts by weight of Radix Paeoniae Alba, 5-15 parts by weight of Gallus galli, 8-18 parts by weight of Rhizoma Alismatis, 5-10 parts by weight of Lygodium japonicum, 2-8 parts by weight of β-cyclodextrin, 0.5-1.5 parts by weight of poloxamer surfactant, and 2-8 parts by weight of sodium carboxymethyl cellulose; S2, grinding Corydalis yanhusuo, white peony root, chicken gizzard lining, oriental water chestnut, and Lygodium japonicum with a grinder to obtain a mixed powder; S3, β-cyclodextrin is dissolved in deionized water to form a 10-20% β-cyclodextrin solution; sodium carboxymethyl cellulose is dissolved in deionized water to form a 0.5-1% sodium carboxymethyl cellulose solution; Sodium carboxymethyl cellulose will form local high-viscosity areas in the early stage of dissolution. If it is directly mixed with β-cyclodextrin, these high-viscosity areas may wrap around β-cyclodextrin, affecting its dissolution and uniform dispersion; the cavity structure of β-cyclodextrin requires appropriate conditions to effectively include drug molecules. If it is directly mixed with high-viscosity sodium carboxymethyl cellulose, it may affect the inclusion capacity of β-cyclodextrin; first mix the drug with β-cyclodextrin to ensure that the drug molecules fully enter the cavity of β-cyclodextrin, and then add sodium carboxymethyl cellulose to improve the inclusion efficiency; and separate dissolution can reduce cross-contamination between different components and ensure the purity and stability of each component.

[0014] Dispersing the Lysimachia sibiricum nanoparticles and the mixed powder in S2 in deionized water, adding poloxamer surfactant, and stirring until completely dissolved to form a suspension; Poloxamer is a non-ionic surfactant with good solubilizing ability. It can reduce the interfacial tension between drugs and water, help poorly soluble drugs to dissolve better in water, and improve the solubility of drugs. Poloxamer can change the behavior of drugs in the digestive tract, improve the absorption efficiency of drugs in the intestine, and transport drugs to the intestinal wall by forming micelles, thereby increasing the bioavailability of drugs. Poloxamer has certain antioxidant properties, which can reduce the oxidative deterioration of drugs during preparation and storage, and extend the shelf life of drugs. Poloxamer can improve the compatibility between drugs and excipients such as β-cyclodextrin and sodium carboxymethyl cellulose, ensuring the uniform dispersion of each component in the solution.

[0015] S4, adding the suspension to the β-cyclodextrin solution, stirring at 30-40°C at a speed of 300-400 rpm for 15-30 min, and adjusting the pH value of the solution to 6.0-7.5 with 0.3-0.5 mol / L phosphate buffer; The appropriate pH value helps the solid particles in the suspension to dissolve or disperse better in the β-cyclodextrin solution; for some poorly soluble substances, specific pH conditions may change their surface charge or intermolecular forces, making it easier for them to interact with solvent molecules or β-cyclodextrin, thereby improving their dispersibility in the solution and facilitating subsequent reactions or mixing processes.

[0016] Then add it to the sodium carboxymethyl cellulose solution and continue to stir evenly to ensure that the drug and carrier material are fully mixed; β-cyclodextrin has a unique ring structure, which can encapsulate some volatile and unstable components (terpenoids, volatile oils, etc.) in the Lysimachia nanoparticles, protecting them from external environmental factors (such as light, heat, humidity, etc.), thereby improving the stability of the entire composition; sodium carboxymethyl cellulose is a polymer compound that can form a certain network structure in the solution. This network structure can adsorb and fix the active ingredients in traditional Chinese medicine, prevent them from agglomerating or settling, and help maintain the stability of the traditional Chinese medicine composition during storage and use; The combination of β-cyclodextrin and sodium carboxymethyl cellulose can regulate the release rate of the Chinese medicine composition. They can slow down the release rate of the ingredients to a certain extent, so that the drugs can continue to play a role in the body; at the same time, they can ensure rapid release under appropriate conditions so that the drugs can take effect quickly. For example, the active ingredients in the Centella asiatica nanoparticles can be quickly released to exert their efficacy after entering the body.

[0017] S5, pouring the mixed liquid into a pre-cooled mold, cooling and solidifying at room temperature, and taking out the solidified product after the mixed liquid is completely solidified; The solidified material is crushed by a pulverizer, the pulverizer speed is set to 1000-3000 rpm, the time is continued for 10-15 minutes, and particles with a particle size of 60-80 μm are obtained; S6. Freeze-drying the particles to obtain a Chinese medicinal composition for rapidly relieving renal colic.

[0018] Preferably, in S2, the rotation speed of the pulverizer is set to 1000-3000 rpm, the duration is 5-8 min, and the particle size is 100-200 mesh.

[0019] Preferably, in S3, the stirring is performed by a homogenizer at a stirring speed of 8000-10000 rpm and a stirring time of 10-15 min.

[0020] Preferably, in S4, the stirring speed is 400-500 rpm, and the stirring time is 30-40 min.

[0021] Preferably, the freeze-drying temperature is set at -40--50°C, and the drying time is 6-12 hours.

[0022] Compared with the prior art, the present invention has the following beneficial effects: 1. In the preparation method of the Chinese medicine composition for rapidly relieving renal colic, the water solubility of flavonoid glycosides can be increased by extracting and glycosylating flavonoid glycosides, thereby improving their absorption and utilization efficiency in the body, and thus improving their efficacy; the small size of the Lysimachia nanoparticles can significantly increase their absorption area in the digestive tract, and can efficiently transport active ingredients (flavonoids, alkaloids, volatile oils, etc.) to the lesion site, thereby increasing their local concentration in the lesion site and enhancing their efficacy; 2. In the preparation method of the Chinese medicine composition for quickly relieving renal colic, β-cyclodextrin and sodium carboxymethyl cellulose are used as carriers. The combination of β-cyclodextrin and sodium carboxymethyl cellulose can regulate the release rate of the Chinese medicine composition. They can slow down the release rate of the ingredients to a certain extent, so that the drug can continue to play a role in the body; at the same time, they can ensure rapid release under appropriate conditions, so that the drug can take effect quickly; and poloxamer surfactants can improve the compatibility between the drug and auxiliary materials such as β-cyclodextrin and sodium carboxymethyl cellulose, and increase the solubility of the drug. DETAILED DESCRIPTION

[0023] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0024] The Chinese medicine composition for rapidly relieving renal colic of the present invention comprises the following components: 10-30 parts by weight of Lysimachia cyrtonema nanoparticles, 6-16 parts by weight of Corydalis yanhusuo, 8-18 parts by weight of Paeonia lactiflora, 5-15 parts by weight of Gallus gallus domestica, 8-18 parts by weight of Rhizoma alismatis, 5-10 parts by weight of Lygodium japonicum, 2-8 parts by weight of β-cyclodextrin, 0.5-1.5 parts by weight of poloxamer surfactant, and 2-8 parts by weight of sodium carboxymethyl cellulose; The Lysimachia nanoparticles are prepared by glycosylation reaction of flavonoid glycosides in the Lysimachia with sugar, followed by emulsification into nanoparticles; the sugar is glucose.

[0025] Embodiment 1: A method for preparing a Chinese medicine composition for rapidly relieving renal colic, comprising the following steps: S1. Weigh the following components in parts by weight respectively: 20 parts by weight of Lysimachia sibiricum nanoparticles, 11 parts by weight of Corydalis yanhusuo, 13 parts by weight of Radix Paeoniae Alba, 10 parts by weight of Gallus galli, 13 parts by weight of Rhizoma Alismatis, 8 parts by weight of Lygodium japonicum, 6 parts by weight of β-cyclodextrin, 1.0 parts by weight of poloxamer surfactant, and 6 parts by weight of sodium carboxymethyl cellulose; S2, pulverize Corydalis yanhusuo, white peony root, chicken gizzard lining, oriental rhizome, and Lygodium japonicum with a pulverizer, set the speed to 2000 rpm, and last for 8 min to obtain a mixed powder with a particle size of 150 mesh; S3, β-cyclodextrin is dissolved in deionized water to form a 10% β-cyclodextrin solution; sodium carboxymethyl cellulose is dissolved in deionized water to form a 1% sodium carboxymethyl cellulose solution; The Lysimachia nanoparticles and the mixed powder in S2 were dispersed in deionized water, and a poloxamer surfactant was added, and the mixture was stirred with a homogenizer until completely dissolved at a stirring speed of 8000 rpm for 15 min to form a suspension; S4, adding the suspension to the β-cyclodextrin solution, stirring at 350 rpm for 30 min at 35°C, and adjusting the pH value of the solution to 7.0 with 0.5 mol / L phosphate buffer; After stirring evenly, ultrasonic treatment was performed with an ultrasonic power of 200 W and an ultrasonic time of 10 min, and then the mixture was allowed to stand for 2 h to allow the drug to be fully included in the cavity of β-cyclodextrin; Then add it to the sodium carboxymethyl cellulose solution and continue to stir evenly at a stirring speed of 400 rpm for 30 min to ensure that the drug and carrier material are fully mixed; S5, pouring the mixed liquid into a pre-cooled mold, cooling and solidifying at room temperature, and taking out the solidified product after the mixed liquid is completely solidified; The solidified material was crushed by a pulverizer, the pulverizer speed was set to 3000 rpm, the time lasted for 15 min, and particles with a particle size of 60 μm were obtained; S6. Freeze-drying the particles at a temperature of -50°C for 10 hours to obtain a Chinese medicine composition for rapidly relieving renal colic.

[0026] The preparation of Lysimachia sibiricum nanoparticles is as follows: S1.1, weigh the following components by weight respectively: 10 parts by weight of Lysimachia sibiricum, 50 parts by weight of ethanol, 3 parts by weight of glucose, 1.5 parts by weight of citric acid catalyst, 30 parts by weight of ethyl acetate solvent, and 10 parts by weight of polyvinyl alcohol surfactant; S1.2, crush the Herba Lysimachiae with a grinder at a speed of 2000 rpm for 5 min to obtain Herba Lysimachiae powder with a particle size of 120 mesh; The desmodium powder was dissolved in 70% ethanol, and the mixture was heated under reflux at 65° C. for 3 h to obtain an extract. After the extract was cooled to room temperature, it was filtered to obtain a filtrate. The filtrate was placed in a rotary evaporator for concentration at a temperature of 60°C and a rotation speed of 100 rpm to obtain flavonoid glycosides; S1.3, flavonoid glycosides and glucose were dissolved in deionized water, and stirred at 300 rpm for 30 min at 40°C using a magnetic stirrer to obtain a mixed solution; The citric acid catalyst was added to the mixed solution and stirred continuously at a speed of 200 rpm for 30 min. The glycosylation reaction was carried out at 60°C for 12 h. S1.4. After the reaction is completed, the reaction mixture is filtered; the filtrate is placed in a rotary evaporator at a temperature of 60°C and a rotation speed of 100 rpm to obtain flavonoid glycosides; The flavonoid glycoside was dissolved in ethyl acetate, and the polyvinyl alcohol surfactant was dissolved in water; the two solutions were mixed and stirred vigorously, and the stirring speed was 1000 rpm at room temperature for 20 minutes, and the mixture was placed in a fume hood to form a nanoparticle suspension with a particle size of 100 μm; S1.5. Centrifuge the nanoparticle suspension at a speed of 2000 rpm for 15 min, and wash the nanoparticles with deionized water; vacuum dry the nanoparticles at 50° C. for 12 h to obtain Lysimachia sibiricum nanoparticles.

[0027] Embodiment 2: A method for preparing a Chinese medicine composition for rapidly relieving renal colic, comprising the following steps: S1. Weigh the following components in parts by weight respectively: 20 parts by weight of Lysimachia sibiricum nanoparticles, 11 parts by weight of Corydalis yanhusuo, 13 parts by weight of Radix Paeoniae Alba, 10 parts by weight of Gallus galli, 13 parts by weight of Rhizoma Alismatis, 8 parts by weight of Lygodium japonicum, 6 parts by weight of β-cyclodextrin, 1.0 parts by weight of poloxamer surfactant, and 6 parts by weight of sodium carboxymethyl cellulose; S2, pulverize Corydalis yanhusuo, white peony root, chicken gizzard lining, oriental rhizome, and Lygodium japonicum with a pulverizer, set the speed to 2000 rpm, and last for 8 min to obtain a mixed powder with a particle size of 150 mesh; S3, β-cyclodextrin is dissolved in deionized water to form a 10% β-cyclodextrin solution; sodium carboxymethyl cellulose is dissolved in deionized water to form a 1% sodium carboxymethyl cellulose solution; The Lysimachia nanoparticles and the mixed powder in S2 were dispersed in deionized water, and a poloxamer surfactant was added, and the mixture was stirred with a homogenizer until completely dissolved at a stirring speed of 8000 rpm for 15 min to form a suspension; S4, adding the suspension to the β-cyclodextrin solution, stirring at 350 rpm for 30 min at 35°C, and adjusting the pH value of the solution to 7.0 with 0.5 mol / L phosphate buffer; After stirring evenly, ultrasonic treatment was performed with an ultrasonic power of 200 W and an ultrasonic time of 10 min, and then the mixture was allowed to stand for 2 h to allow the drug to be fully included in the cavity of β-cyclodextrin; Then add it to the sodium carboxymethyl cellulose solution and continue to stir evenly at a stirring speed of 400 rpm for 30 min to ensure that the drug and carrier material are fully mixed; S5, pouring the mixed liquid into a pre-cooled mold, cooling and solidifying at room temperature, and taking out the solidified product after the mixed liquid is completely solidified; The solidified material was crushed by a pulverizer, the pulverizer speed was set to 3000 rpm, the time lasted for 15 min, and particles with a particle size of 60 μm were obtained; S6. Freeze-drying the particles at a temperature of -50°C for 10 hours to obtain a Chinese medicine composition for rapidly relieving renal colic.

[0028] The preparation of Lysimachia sibiricum nanoparticles is as follows: S1.1, weigh the following components in parts by weight respectively: 10 parts by weight of Lysimachia sibiricum, 50 parts by weight of ethanol, 5 parts by weight of glucose, 1.5 parts by weight of citric acid catalyst, 30 parts by weight of ethyl acetate solvent, and 10 parts by weight of polyvinyl alcohol surfactant; S1.2, crush the Herba Lysimachiae with a grinder at a speed of 2000 rpm for 5 min to obtain Herba Lysimachiae powder with a particle size of 120 mesh; The desmodium powder was dissolved in 70% ethanol, and the mixture was heated under reflux at 65° C. for 3 h to obtain an extract. After the extract was cooled to room temperature, it was filtered to obtain a filtrate. The filtrate was placed in a rotary evaporator for concentration at a temperature of 60°C and a rotation speed of 100 rpm to obtain flavonoid glycosides; S1.3, flavonoid glycosides and glucose were dissolved in deionized water, and stirred at 300 rpm for 30 min at 40°C using a magnetic stirrer to obtain a mixed solution; The citric acid catalyst was added to the mixed solution and stirred continuously at a speed of 200 rpm for 30 min. The glycosylation reaction was carried out at 60°C for 12 h. S1.4. After the reaction is completed, the reaction mixture is filtered; the filtrate is placed in a rotary evaporator at a temperature of 60°C and a rotation speed of 100 rpm to obtain flavonoid glycosides; The flavonoid glycoside was dissolved in ethyl acetate, and the polyvinyl alcohol surfactant was dissolved in water; the two solutions were mixed and stirred vigorously, and the stirring speed was 1000 rpm at room temperature for 20 minutes, and the mixture was placed in a fume hood to form a nanoparticle suspension with a particle size of 100 μm; S1.5. Centrifuge the nanoparticle suspension at a speed of 2000 rpm for 15 min, and wash the nanoparticles with deionized water; vacuum dry the nanoparticles at 50° C. for 12 h to obtain Lysimachia sibiricum nanoparticles.

[0029] Embodiment 3: A method for preparing a Chinese medicine composition for rapidly relieving renal colic, comprising the following steps: S1. Weigh the following components in parts by weight respectively: 20 parts by weight of Lysimachia sibiricum nanoparticles, 11 parts by weight of Corydalis yanhusuo, 13 parts by weight of Radix Paeoniae Alba, 10 parts by weight of Gallus galli, 13 parts by weight of Rhizoma Alismatis, 8 parts by weight of Lygodium japonicum, 6 parts by weight of β-cyclodextrin, 1.0 parts by weight of poloxamer surfactant, and 6 parts by weight of sodium carboxymethyl cellulose; S2, pulverize Corydalis yanhusuo, white peony root, chicken gizzard lining, oriental rhizome, and Lygodium japonicum with a pulverizer, set the speed to 2000 rpm, and last for 8 min to obtain a mixed powder with a particle size of 150 mesh; S3, β-cyclodextrin is dissolved in deionized water to form a 10% β-cyclodextrin solution; sodium carboxymethyl cellulose is dissolved in deionized water to form a 1% sodium carboxymethyl cellulose solution; The Lysimachia nanoparticles and the mixed powder in S2 were dispersed in deionized water, and a poloxamer surfactant was added, and the mixture was stirred with a homogenizer until completely dissolved at a stirring speed of 8000 rpm for 15 min to form a suspension; S4, adding the suspension to the β-cyclodextrin solution, stirring at 350 rpm for 30 min at 35°C, and adjusting the pH value of the solution to 7.0 with 0.5 mol / L phosphate buffer; After stirring evenly, ultrasonic treatment was performed with an ultrasonic power of 200 W and an ultrasonic time of 10 min, and then the mixture was allowed to stand for 2 h to allow the drug to be fully included in the cavity of β-cyclodextrin; Then add it to the sodium carboxymethyl cellulose solution and continue to stir evenly at a stirring speed of 400 rpm for 30 min to ensure that the drug and carrier material are fully mixed; S5, pouring the mixed liquid into a pre-cooled mold, cooling and solidifying at room temperature, and taking out the solidified product after the mixed liquid is completely solidified; The solidified material was crushed by a pulverizer, the pulverizer speed was set to 3000 rpm, the time was continued for 15 min, and particles with a particle size of 60 μm were obtained; S6. Freeze-drying the particles at a temperature of -50°C for 10 hours to obtain a Chinese medicine composition for rapidly relieving renal colic.

[0030] The preparation of Lysimachia sibiricum nanoparticles is as follows: S1.1, weigh the following components by weight respectively: 10 parts by weight of Lysimachia sibiricum, 50 parts by weight of ethanol, 7 parts by weight of glucose, 1.5 parts by weight of citric acid catalyst, 30 parts by weight of ethyl acetate solvent, and 10 parts by weight of polyvinyl alcohol surfactant; S1.2, crush the Herba Lysimachiae with a grinder at a speed of 2000 rpm for 5 min to obtain Herba Lysimachiae powder with a particle size of 120 mesh; The desmodium powder was dissolved in 70% ethanol, and heated under reflux at 65° C. for 3 h to obtain an extract. After the extract was cooled to room temperature, it was filtered to obtain a filtrate. The filtrate was placed in a rotary evaporator for concentration at a temperature of 60°C and a rotation speed of 100 rpm to obtain flavonoid glycosides; S1.3, flavonoid glycosides and glucose were dissolved in deionized water, and stirred at 300 rpm for 30 min at 40°C using a magnetic stirrer to obtain a mixed solution; The citric acid catalyst was added to the mixed solution and stirred continuously at a speed of 200 rpm for 30 min. The glycosylation reaction was carried out at 60°C for 12 h. S1.4. After the reaction is completed, the reaction mixture is filtered; the filtrate is placed in a rotary evaporator at a temperature of 60°C and a rotation speed of 100 rpm to obtain flavonoid glycosides; The flavonoid glycoside was dissolved in ethyl acetate, and the polyvinyl alcohol surfactant was dissolved in water; the two solutions were mixed and stirred vigorously, and the stirring speed was 1000 rpm at room temperature for 20 minutes, and the mixture was placed in a fume hood to form a nanoparticle suspension with a particle size of 100 μm; S1.5. Centrifuge the nanoparticle suspension at a speed of 2000 rpm for 15 min, and wash the nanoparticles with deionized water; vacuum dry the nanoparticles at 50° C. for 12 h to obtain Lysimachia sibiricum nanoparticles.

[0031] Embodiment 4: A method for preparing a Chinese medicine composition for rapidly relieving renal colic, comprising the following steps: S1. Weigh the following components in parts by weight respectively: 20 parts by weight of Lysimachia sibiricum nanoparticles, 11 parts by weight of Corydalis yanhusuo, 13 parts by weight of Radix Paeoniae Alba, 10 parts by weight of Gallus galli, 13 parts by weight of Rhizoma Alismatis, 8 parts by weight of Lygodium japonicum, 6 parts by weight of β-cyclodextrin, 1.0 parts by weight of poloxamer surfactant, and 6 parts by weight of sodium carboxymethyl cellulose; S2, pulverize Corydalis yanhusuo, white peony root, chicken gizzard lining, oriental rhizome, and Lygodium japonicum with a pulverizer, set the speed to 2000 rpm, and last for 8 min to obtain a mixed powder with a particle size of 150 mesh; S3, β-cyclodextrin is dissolved in deionized water to form a 10% β-cyclodextrin solution; sodium carboxymethyl cellulose is dissolved in deionized water to form a 1% sodium carboxymethyl cellulose solution; The Lysimachia nanoparticles and the mixed powder in S2 were dispersed in deionized water, and a poloxamer surfactant was added, and the mixture was stirred with a homogenizer until completely dissolved at a stirring speed of 8000 rpm for 15 min to form a suspension; S4, adding the suspension to the β-cyclodextrin solution, stirring at 350 rpm for 30 min at 35°C, and adjusting the pH value of the solution to 7.0 with 0.5 mol / L phosphate buffer; After stirring evenly, ultrasonic treatment was performed with an ultrasonic power of 200 W and an ultrasonic time of 10 min, and then the mixture was allowed to stand for 2 h to allow the drug to be fully included in the cavity of β-cyclodextrin; Then add it to the sodium carboxymethyl cellulose solution and continue to stir evenly at a stirring speed of 400 rpm for 30 min to ensure that the drug and carrier material are fully mixed; S5, pouring the mixed liquid into a pre-cooled mold, cooling and solidifying at room temperature, and taking out the solidified product after the mixed liquid is completely solidified; The solidified material was crushed by a pulverizer, the pulverizer speed was set to 3000 rpm, the time lasted for 15 min, and particles with a particle size of 60 μm were obtained; S6. Freeze-drying the particles at a temperature of -50°C for 10 hours to obtain a Chinese medicine composition for rapidly relieving renal colic.

[0032] The preparation of Lysimachia sibiricum nanoparticles is as follows: S1.1, weigh the following components by weight respectively: 6 parts by weight of Lysimachia sibiricum, 50 parts by weight of ethanol, 3 parts by weight of glucose, 1.5 parts by weight of citric acid catalyst, 30 parts by weight of ethyl acetate solvent, and 10 parts by weight of polyvinyl alcohol surfactant; S1.2, crush the Herba Lysimachiae with a grinder at a speed of 2000 rpm for 5 min to obtain Herba Lysimachiae powder with a particle size of 120 mesh; The desmodium powder was dissolved in 70% ethanol, and the mixture was heated under reflux at 65° C. for 3 h to obtain an extract. After the extract was cooled to room temperature, it was filtered to obtain a filtrate. The filtrate was placed in a rotary evaporator for concentration at a temperature of 60°C and a rotation speed of 100 rpm to obtain flavonoid glycosides; S1.3, flavonoid glycosides and glucose were dissolved in deionized water, and stirred at 300 rpm for 30 min at 40°C using a magnetic stirrer to obtain a mixed solution; The citric acid catalyst was added to the mixed solution and stirred continuously at a speed of 200 rpm for 30 min. The glycosylation reaction was carried out at 60°C for 12 h. S1.4. After the reaction is completed, the reaction mixture is filtered; the filtrate is placed in a rotary evaporator at a temperature of 60°C and a rotation speed of 100 rpm to obtain flavonoid glycosides; The flavonoid glycoside was dissolved in ethyl acetate, and the polyvinyl alcohol surfactant was dissolved in water; the two solutions were mixed and stirred vigorously, and the stirring speed was 1000 rpm at room temperature for 20 minutes, and the mixture was placed in a fume hood to form a nanoparticle suspension with a particle size of 100 μm; S1.5. Centrifuge the nanoparticle suspension at a speed of 2000 rpm for 15 min, and wash the nanoparticles with deionized water; vacuum dry the nanoparticles at 50° C. for 12 h to obtain Lysimachia sibiricum nanoparticles.

[0033] Embodiment 5: A method for preparing a Chinese medicine composition for rapidly relieving renal colic, comprising the following steps: S1. Weigh the following components in parts by weight respectively: 20 parts by weight of Lysimachia sibiricum nanoparticles, 11 parts by weight of Corydalis yanhusuo, 13 parts by weight of Radix Paeoniae Alba, 10 parts by weight of Gallus galli, 13 parts by weight of Rhizoma Alismatis, 8 parts by weight of Lygodium japonicum, 6 parts by weight of β-cyclodextrin, 1.0 parts by weight of poloxamer surfactant, and 6 parts by weight of sodium carboxymethyl cellulose; S2, pulverize Corydalis yanhusuo, white peony root, chicken gizzard lining, oriental rhizome, and Lygodium japonicum with a pulverizer, set the speed to 2000 rpm, and last for 8 min to obtain a mixed powder with a particle size of 150 mesh; S3, β-cyclodextrin is dissolved in deionized water to form a 10% β-cyclodextrin solution; sodium carboxymethyl cellulose is dissolved in deionized water to form a 1% sodium carboxymethyl cellulose solution; The Lysimachia nanoparticles and the mixed powder in S2 were dispersed in deionized water, and a poloxamer surfactant was added, and the mixture was stirred with a homogenizer until completely dissolved at a stirring speed of 8000 rpm for 15 min to form a suspension; S4, adding the suspension to the β-cyclodextrin solution, stirring at 350 rpm for 30 min at 35°C, and adjusting the pH value of the solution to 7.0 with 0.5 mol / L phosphate buffer; After stirring evenly, ultrasonic treatment was performed with an ultrasonic power of 200 W and an ultrasonic time of 10 min, and then the mixture was allowed to stand for 2 h to allow the drug to be fully included in the cavity of β-cyclodextrin; Then add it to the sodium carboxymethyl cellulose solution and continue to stir evenly at a stirring speed of 400 rpm for 30 min to ensure that the drug and carrier material are fully mixed; S5, pouring the mixed liquid into a pre-cooled mold, cooling and solidifying at room temperature, and taking out the solidified product after the mixed liquid is completely solidified; The solidified material was crushed by a pulverizer, the pulverizer speed was set to 3000 rpm, the time lasted for 15 min, and particles with a particle size of 60 μm were obtained; S6. Freeze-drying the particles at a temperature of -50°C for 10 hours to obtain a Chinese medicine composition for rapidly relieving renal colic.

[0034] The preparation of Lysimachia sibiricum nanoparticles is as follows: S1.1, weigh the following components by weight respectively: 10 parts by weight of Lysimachia sibiricum, 40 parts by weight of ethanol, 5 parts by weight of glucose, 0.5 parts by weight of citric acid catalyst, 20 parts by weight of ethyl acetate solvent, and 10 parts by weight of polyvinyl alcohol surfactant; S1.2, crush the Herba Lysimachiae with a grinder at a speed of 2000 rpm for 5 min to obtain Herba Lysimachiae powder with a particle size of 120 mesh; The desmodium powder was dissolved in 70% ethanol, and the mixture was heated under reflux at 65° C. for 3 h to obtain an extract. After the extract was cooled to room temperature, it was filtered to obtain a filtrate. The filtrate was placed in a rotary evaporator for concentration at a temperature of 60°C and a rotation speed of 100 rpm to obtain flavonoid glycosides; S1.3, flavonoid glycosides and glucose were dissolved in deionized water, and stirred at 300 rpm for 30 min at 40°C using a magnetic stirrer to obtain a mixed solution; The citric acid catalyst was added to the mixed solution and stirred continuously at a speed of 200 rpm for 30 min. The glycosylation reaction was carried out at 60°C for 12 h. S1.4. After the reaction is completed, the reaction mixture is filtered; the filtrate is placed in a rotary evaporator at a temperature of 60°C and a rotation speed of 100 rpm to obtain flavonoid glycosides; The flavonoid glycoside was dissolved in ethyl acetate, and the polyvinyl alcohol surfactant was dissolved in water; the two solutions were mixed and stirred vigorously, and the stirring speed was 1000 rpm at room temperature for 20 minutes, and the mixture was placed in a fume hood to form a nanoparticle suspension with a particle size of 100 μm; S1.5. Centrifuge the nanoparticle suspension at a speed of 2000 rpm for 15 min, and wash the nanoparticles with deionized water; vacuum dry the nanoparticles at 50° C. for 12 h to obtain Lysimachia sibiricum nanoparticles.

[0035] Example 6: A method for preparing a Chinese medicine composition for rapidly relieving renal colic, comprising the following steps: S1. Weigh the following components in parts by weight respectively: 20 parts by weight of Lysimachia sibiricum nanoparticles, 11 parts by weight of Corydalis yanhusuo, 13 parts by weight of Radix Paeoniae Alba, 10 parts by weight of Gallus galli, 13 parts by weight of Rhizoma Alismatis, 8 parts by weight of Lygodium japonicum, 6 parts by weight of β-cyclodextrin, 0.5 parts by weight of poloxamer surfactant, and 6 parts by weight of sodium carboxymethylcellulose; S2, pulverize Corydalis yanhusuo, white peony root, chicken gizzard lining, oriental rhizome, and Lygodium japonicum with a pulverizer, set the speed to 2000 rpm, and last for 8 min to obtain a mixed powder with a particle size of 150 mesh; S3, β-cyclodextrin is dissolved in deionized water to form a 10% β-cyclodextrin solution; sodium carboxymethyl cellulose is dissolved in deionized water to form a 1% sodium carboxymethyl cellulose solution; The Lysimachia nanoparticles and the mixed powder in S2 were dispersed in deionized water, and a poloxamer surfactant was added, and the mixture was stirred with a homogenizer until completely dissolved at a stirring speed of 8000 rpm for 15 min to form a suspension; S4, adding the suspension to the β-cyclodextrin solution, stirring at 350 rpm for 30 min at 35°C, and adjusting the pH value of the solution to 7.0 with 0.5 mol / L phosphate buffer; After stirring evenly, ultrasonic treatment was performed with an ultrasonic power of 200 W and an ultrasonic time of 10 min, and then the mixture was allowed to stand for 2 h to allow the drug to be fully included in the cavity of β-cyclodextrin; Then add it to the sodium carboxymethyl cellulose solution and continue to stir evenly at a stirring speed of 400 rpm for 30 min to ensure that the drug and carrier material are fully mixed; S5, pouring the mixed liquid into a pre-cooled mold, cooling and solidifying at room temperature, and taking out the solidified product after the mixed liquid is completely solidified; The solidified material was crushed by a pulverizer, the pulverizer speed was set to 3000 rpm, the time lasted for 15 min, and particles with a particle size of 60 μm were obtained; S6. Freeze-drying the particles at a temperature of -50°C for 10 hours to obtain a Chinese medicine composition for rapidly relieving renal colic.

[0036] The preparation of Lysimachia sibiricum nanoparticles is as follows: S1.1, weigh the following components by weight respectively: 10 parts by weight of Lysimachia sibiricum, 50 parts by weight of ethanol, 5 parts by weight of glucose, 1.5 parts by weight of citric acid catalyst, 30 parts by weight of ethyl acetate solvent, and 5 parts by weight of polyvinyl alcohol surfactant; S1.2, crush the Herba Lysimachiae with a grinder at a speed of 2000 rpm for 5 min to obtain Herba Lysimachiae powder with a particle size of 120 mesh; The desmodium powder was dissolved in 70% ethanol, and heated under reflux at 65° C. for 3 h to obtain an extract. After the extract was cooled to room temperature, it was filtered to obtain a filtrate. The filtrate was placed in a rotary evaporator for concentration at a temperature of 60°C and a rotation speed of 100 rpm to obtain flavonoid glycosides; S1.3, flavonoid glycosides and glucose were dissolved in deionized water, and stirred at 300 rpm for 30 min at 40°C using a magnetic stirrer to obtain a mixed solution; The citric acid catalyst was added to the mixed solution and stirred continuously at a speed of 200 rpm for 30 min. The glycosylation reaction was carried out at 60°C for 12 h. S1.4. After the reaction is completed, the reaction mixture is filtered; the filtrate is placed in a rotary evaporator at a temperature of 60°C and a rotation speed of 100 rpm to obtain flavonoid glycosides; The flavonoid glycoside was dissolved in ethyl acetate, and the polyvinyl alcohol surfactant was dissolved in water; the two solutions were mixed and stirred vigorously, and the stirring speed was 1000 rpm at room temperature for 20 minutes, and the mixture was placed in a fume hood to form a nanoparticle suspension with a particle size of 100 μm; S1.5. Centrifuge the nanoparticle suspension at a speed of 2000 rpm for 15 min, and wash the nanoparticles with deionized water; vacuum dry the nanoparticles at 50° C. for 12 h to obtain Lysimachia sibiricum nanoparticles.

[0037] Example 7: A method for preparing a Chinese medicine composition for rapidly relieving renal colic, comprising the following steps: S1. Weigh the following components in parts by weight respectively: 20 parts by weight of Lysimachia sibiricum nanoparticles, 11 parts by weight of Corydalis yanhusuo, 13 parts by weight of Radix Paeoniae Alba, 10 parts by weight of Gallus galli, 13 parts by weight of Rhizoma Alismatis, 8 parts by weight of Lygodium japonicum, 2 parts by weight of β-cyclodextrin, 1.0 parts by weight of poloxamer surfactant, and 2 parts by weight of sodium carboxymethylcellulose; S2, pulverize Corydalis yanhusuo, white peony root, chicken gizzard lining, oriental rhizome, and Lygodium japonicum with a pulverizer, set the speed to 2000 rpm, and last for 8 min to obtain a mixed powder with a particle size of 150 mesh; S3, β-cyclodextrin is dissolved in deionized water to form a 10% β-cyclodextrin solution; sodium carboxymethyl cellulose is dissolved in deionized water to form a 1% sodium carboxymethyl cellulose solution; The Lysimachia nanoparticles and the mixed powder in S2 were dispersed in deionized water, and a poloxamer surfactant was added, and the mixture was stirred with a homogenizer until completely dissolved at a stirring speed of 8000 rpm for 15 min to form a suspension; S4, adding the suspension to the β-cyclodextrin solution, stirring at 350 rpm for 30 min at 35°C, and adjusting the pH value of the solution to 7.0 with 0.5 mol / L phosphate buffer; After stirring evenly, ultrasonic treatment was performed with an ultrasonic power of 200 W and an ultrasonic time of 10 min, and then the mixture was allowed to stand for 2 h to allow the drug to be fully included in the cavity of β-cyclodextrin; Then add it to the sodium carboxymethyl cellulose solution and continue to stir evenly at a stirring speed of 400 rpm for 30 min to ensure that the drug and carrier material are fully mixed; S5, pouring the mixed liquid into a pre-cooled mold, cooling and solidifying at room temperature, and taking out the solidified product after the mixed liquid is completely solidified; The solidified material was crushed by a pulverizer, the pulverizer speed was set to 3000 rpm, the time was continued for 15 min, and particles with a particle size of 60 μm were obtained; S6. Freeze-drying the particles at a temperature of -50°C for 10 hours to obtain a Chinese medicine composition for rapidly relieving renal colic.

[0038] The preparation of Lysimachia sibiricum nanoparticles is as follows: S1.1, weigh the following components in parts by weight respectively: 10 parts by weight of Lysimachia sibiricum, 50 parts by weight of ethanol, 5 parts by weight of glucose, 1.5 parts by weight of citric acid catalyst, 30 parts by weight of ethyl acetate solvent, and 10 parts by weight of polyvinyl alcohol surfactant; S1.2, crush the Herba Lysimachiae with a grinder at a speed of 2000 rpm for 5 min to obtain Herba Lysimachiae powder with a particle size of 120 mesh; The desmodium powder was dissolved in 70% ethanol, and heated under reflux at 65° C. for 3 h to obtain an extract. After the extract was cooled to room temperature, it was filtered to obtain a filtrate. The filtrate was placed in a rotary evaporator for concentration at a temperature of 60°C and a rotation speed of 100 rpm to obtain flavonoid glycosides; S1.3, flavonoid glycosides and glucose were dissolved in deionized water, and stirred at 300 rpm for 30 min at 40°C using a magnetic stirrer to obtain a mixed solution; The citric acid catalyst was added to the mixed solution and stirred continuously at a speed of 200 rpm for 30 min. The glycosylation reaction was carried out at 60°C for 12 h. S1.4. After the reaction is completed, the reaction mixture is filtered; the filtrate is placed in a rotary evaporator at a temperature of 60°C and a rotation speed of 100 rpm to obtain flavonoid glycosides; The flavonoid glycoside was dissolved in ethyl acetate, and the polyvinyl alcohol surfactant was dissolved in water; the two solutions were mixed and stirred vigorously, and the stirring speed was 1000 rpm at room temperature for 20 minutes, and the mixture was placed in a fume hood to form a nanoparticle suspension with a particle size of 100 μm; S1.5. Centrifuge the nanoparticle suspension at a speed of 2000 rpm for 15 min, and wash the nanoparticles with deionized water; vacuum dry the nanoparticles at 50° C. for 12 h to obtain Lysimachia sibiricum nanoparticles.

[0039] Embodiment 8: A method for preparing a Chinese medicine composition for rapidly relieving renal colic, comprising the following steps: S1. Weigh the following components in parts by weight respectively: 10 parts by weight of Lysimachia sibiricum nanoparticles, 11 parts by weight of Corydalis yanhusuo, 13 parts by weight of Radix Paeoniae Alba, 10 parts by weight of Gallus galli, 13 parts by weight of Rhizoma Alismatis, 8 parts by weight of Lygodium japonicum, 6 parts by weight of β-cyclodextrin, 1.0 parts by weight of poloxamer surfactant, and 6 parts by weight of sodium carboxymethyl cellulose; S2, pulverize Corydalis yanhusuo, white peony root, chicken gizzard lining, oriental rhizome, and Lygodium japonicum with a pulverizer, set the speed to 2000 rpm, and last for 8 min to obtain a mixed powder with a particle size of 150 mesh; S3, β-cyclodextrin is dissolved in deionized water to form a 10% β-cyclodextrin solution; sodium carboxymethyl cellulose is dissolved in deionized water to form a 1% sodium carboxymethyl cellulose solution; The Lysimachia nanoparticles and the mixed powder in S2 were dispersed in deionized water, and a poloxamer surfactant was added, and the mixture was stirred with a homogenizer until completely dissolved at a stirring speed of 8000 rpm for 15 min to form a suspension; S4, adding the suspension to the β-cyclodextrin solution, stirring at 350 rpm for 30 min at 35°C, and adjusting the pH value of the solution to 7.0 with 0.5 mol / L phosphate buffer; After stirring evenly, ultrasonic treatment was performed with an ultrasonic power of 200 W and an ultrasonic time of 10 min, and then the mixture was allowed to stand for 2 h to allow the drug to be fully included in the cavity of β-cyclodextrin; Then add it to the sodium carboxymethyl cellulose solution and continue to stir evenly at a stirring speed of 400 rpm for 30 min to ensure that the drug and carrier material are fully mixed; S5, pouring the mixed liquid into a pre-cooled mold, cooling and solidifying at room temperature, and taking out the solidified product after the mixed liquid is completely solidified; The solidified material was crushed by a pulverizer, the pulverizer speed was set to 3000 rpm, the time lasted for 15 min, and particles with a particle size of 60 μm were obtained; S6. Freeze-drying the particles at a temperature of -50°C for 10 hours to obtain a Chinese medicine composition for rapidly relieving renal colic.

[0040] The preparation of Lysimachia sibiricum nanoparticles is as follows: S1.1, weigh the following components in parts by weight respectively: 10 parts by weight of Lysimachia sibiricum, 50 parts by weight of ethanol, 5 parts by weight of glucose, 1.5 parts by weight of citric acid catalyst, 30 parts by weight of ethyl acetate solvent, and 10 parts by weight of polyvinyl alcohol surfactant; S1.2, crush the Herba Lysimachiae with a grinder at a speed of 2000 rpm for 5 min to obtain Herba Lysimachiae powder with a particle size of 120 mesh; The desmodium powder was dissolved in 70% ethanol, and the mixture was heated under reflux at 65° C. for 3 h to obtain an extract. After the extract was cooled to room temperature, it was filtered to obtain a filtrate. The filtrate was placed in a rotary evaporator for concentration at a temperature of 60°C and a rotation speed of 100 rpm to obtain flavonoid glycosides; S1.3, flavonoid glycosides and glucose were dissolved in deionized water, and stirred at 300 rpm for 30 min at 40°C using a magnetic stirrer to obtain a mixed solution; The citric acid catalyst was added to the mixed solution and stirred continuously at a speed of 200 rpm for 30 min. The glycosylation reaction was carried out at 60°C for 12 h. S1.4. After the reaction is completed, the reaction mixture is filtered; the filtrate is placed in a rotary evaporator at a temperature of 60°C and a rotation speed of 100 rpm to obtain flavonoid glycosides; The flavonoid glycoside was dissolved in ethyl acetate, and the polyvinyl alcohol surfactant was dissolved in water; the two solutions were mixed and stirred vigorously, and the stirring speed was 1000 rpm at room temperature for 20 minutes, and the mixture was placed in a fume hood to form a nanoparticle suspension with a particle size of 100 μm; S1.5. Centrifuge the nanoparticle suspension at a speed of 2000 rpm for 15 min, and wash the nanoparticles with deionized water; vacuum dry the nanoparticles at 50° C. for 12 h to obtain Lysimachia sibiricum nanoparticles.

[0041] Example 9: A method for preparing a Chinese medicine composition for rapidly relieving renal colic, comprising the following steps: S1. Weigh the following components in parts by weight respectively: 20 parts by weight of Lysimachia cyrtonema nanoparticles, 6 parts by weight of Corydalis yanhusuo, 8 parts by weight of Radix Paeoniae Alba, 5 parts by weight of Gallus gallus domestica, 8 parts by weight of Rhizoma Alismatis, 5 parts by weight of Lygodium japonicum, 6 parts by weight of β-cyclodextrin, 1.0 parts by weight of poloxamer surfactant, and 6 parts by weight of sodium carboxymethyl cellulose; S2, pulverize Corydalis yanhusuo, white peony root, chicken gizzard lining, oriental rhizome, and Lygodium japonicum with a pulverizer, set the speed to 2000 rpm, and last for 8 min to obtain a mixed powder with a particle size of 150 mesh; S3, β-cyclodextrin is dissolved in deionized water to form a 10% β-cyclodextrin solution; sodium carboxymethyl cellulose is dissolved in deionized water to form a 1% sodium carboxymethyl cellulose solution; The Lysimachia nanoparticles and the mixed powder in S2 were dispersed in deionized water, and a poloxamer surfactant was added, and the mixture was stirred with a homogenizer until completely dissolved at a stirring speed of 8000 rpm for 15 min to form a suspension; S4, adding the suspension to the β-cyclodextrin solution, stirring at 350 rpm for 30 min at 35°C, and adjusting the pH value of the solution to 7.0 with 0.5 mol / L phosphate buffer; After stirring evenly, ultrasonic treatment was performed with an ultrasonic power of 200 W and an ultrasonic time of 10 min, and then the mixture was allowed to stand for 2 h to allow the drug to be fully included in the cavity of β-cyclodextrin; Then add it to the sodium carboxymethyl cellulose solution and continue to stir evenly at a stirring speed of 400 rpm for 30 min to ensure that the drug and carrier material are fully mixed; S5, pouring the mixed liquid into a pre-cooled mold, cooling and solidifying at room temperature, and taking out the solidified product after the mixed liquid is completely solidified; The solidified material was crushed by a pulverizer, the pulverizer speed was set to 3000 rpm, the time lasted for 15 min, and particles with a particle size of 60 μm were obtained; S6. Freeze-drying the particles at a temperature of -50°C for 10 hours to obtain a Chinese medicine composition for rapidly relieving renal colic.

[0042] The preparation of Lysimachia sibiricum nanoparticles is as follows: S1.1, weigh the following components in parts by weight respectively: 10 parts by weight of Lysimachia sibiricum, 50 parts by weight of ethanol, 5 parts by weight of glucose, 1.5 parts by weight of citric acid catalyst, 30 parts by weight of ethyl acetate solvent, and 10 parts by weight of polyvinyl alcohol surfactant; S1.2, crush the Herba Lysimachiae with a grinder at a speed of 2000 rpm for 5 min to obtain Herba Lysimachiae powder with a particle size of 120 mesh; The desmodium powder was dissolved in 70% ethanol, and the mixture was heated under reflux at 65° C. for 3 h to obtain an extract. After the extract was cooled to room temperature, it was filtered to obtain a filtrate. The filtrate was placed in a rotary evaporator for concentration at a temperature of 60°C and a rotation speed of 100 rpm to obtain flavonoid glycosides; S1.3, flavonoid glycosides and glucose were dissolved in deionized water, and stirred at 300 rpm for 30 min at 40°C using a magnetic stirrer to obtain a mixed solution; The citric acid catalyst was added to the mixed solution and stirred continuously at a speed of 200 rpm for 30 min. The glycosylation reaction was carried out at 60°C for 12 h. S1.4. After the reaction is completed, the reaction mixture is filtered; the filtrate is placed in a rotary evaporator at a temperature of 60°C and a rotation speed of 100 rpm to obtain flavonoid glycosides; The flavonoid glycoside was dissolved in ethyl acetate, and the polyvinyl alcohol surfactant was dissolved in water; the two solutions were mixed and stirred vigorously, and the stirring speed was 1000 rpm at room temperature for 20 minutes, and the mixture was placed in a fume hood to form a nanoparticle suspension with a particle size of 100 μm; S1.5. Centrifuge the nanoparticle suspension at a speed of 2000 rpm for 15 min, and wash the nanoparticles with deionized water; vacuum dry the nanoparticles at 50° C. for 12 h to obtain Lysimachia sibiricum nanoparticles.

[0043] The method of Example 2 is adopted to remove either β-cyclodextrin or sodium carboxymethyl cellulose.

[0044] The method of Example 2 was used to remove the poloxamer surfactant.

[0045] The method of Example 2 was adopted without using Lysimachia sibiricum nanoparticles.

[0046] The present invention discloses a Chinese medicine composition for rapidly relieving renal colic prepared by using Lysimachia sibiricum nanoparticles, wherein the effect of the Chinese medicine composition is tested as follows: Assessment of pain severity: The visual analogue scale can be used to observe changes in the scores of patients 30 minutes after using the Chinese medicine composition, with 0 representing no pain and 10 representing the most severe pain.

[0047] Pain relief time: record the time from when the patient starts taking the Chinese medicine composition to when the pain is significantly relieved (such as the pain level is reduced by more than 50%).

[0048] Cumulative dissolution percentage: The paddle method (placing the drug sample in the dissolution medium, stirring with a paddle stirrer at a specific speed, and regularly sampling to determine the drug concentration until the drug is completely dissolved) is used to regularly sample and determine the amount of drug dissolved at different time points to calculate the cumulative dissolution percentage.

[0049] According to the above standards, the Chinese medicine compositions prepared in the above Examples 1-9 and Comparative Examples 1-3 were tested, and the obtained data are shown in Table 1: Table 1 Data of the Chinese medicine compositions of Examples 1-9 and Comparative Examples 1-3

[0050] It can be seen from Example 2 in combination with Example 7 and Comparative Example 1 that when either β-cyclodextrin or sodium carboxymethyl cellulose is removed, the relief time of the Chinese medicine composition is significantly prolonged, the relief degree is significantly reduced, and the cumulative dissolution percentage is significantly reduced; β-cyclodextrin and sodium carboxymethyl cellulose can improve the stability of drugs by forming inclusion complexes or other forms of complexes with drug molecules, and can also improve the bioavailability of drugs by improving the solubility and permeability of drugs; if any of them is removed, the bioavailability of drugs may decrease, thereby affecting the relief time, relief degree and cumulative dissolution percentage of drugs; β-cyclodextrin is a good inclusion material. It can form inclusion complexes with the insoluble components in traditional Chinese medicine to increase the solubility of the drug. When β-cyclodextrin is removed, the solubility of the originally included insoluble drug components decreases, resulting in a slower dissolution rate of the drug in the body and delayed absorption, thereby prolonging the drug's effect time and significantly prolonging the relief time. At the same time, since the drug cannot reach an effective blood concentration in time, its relief degree will also be significantly reduced. Some traditional Chinese medicine components may be easily degraded or chemically reacted in the gastrointestinal environment and inactivated. β-cyclodextrin can protect these components. Once removed, the drug components may be destroyed in the gastrointestinal tract due to environmental factors (such as gastric acid, enzymes, etc.), reducing the amount of drug that can exert its efficacy, resulting in a decrease in the relief degree. Sodium carboxymethyl cellulose is a commonly used suspending agent and thickening agent. In traditional Chinese medicine compositions, it plays a key role in some suspension preparations or dosage forms that require a certain viscosity to ensure the distribution and residence time of the drug in the gastrointestinal tract. If sodium carboxymethyl cellulose is removed, the drug may settle quickly and cannot be evenly distributed in the gastrointestinal tract, affecting the contact area and contact time between the drug and the gastrointestinal mucosa, resulting in incomplete drug absorption, prolonged relief time, and reduced relief degree.

[0051] It can be seen from Example 2 and Example 6 combined with Comparative Example 2 that when the poloxamer surfactant is removed, the relief time of the Chinese medicine composition is significantly prolonged, the degree of relief is significantly reduced, and the cumulative dissolution percentage is significantly reduced; Poloxamer can reduce the interfacial tension between drugs and water, help poorly soluble drugs dissolve better in water, improve the solubility of drugs, form stable micelles, prevent the aggregation and sedimentation of drug particles, and maintain the uniformity and stability of the suspension. When poloxamer is removed, the solubility of the drug decreases, resulting in a decrease in the cumulative dissolution percentage, a slowdown in the absorption of the drug in the body, and a prolonged relief time; poloxamer can improve the compatibility between drugs and excipients such as β-cyclodextrin and sodium carboxymethyl cellulose, ensuring the uniform dispersion of each component in the solution. Poloxamer can reduce adverse interactions between drugs and excipients and avoid the reduction of drug activity.

[0052] Through Examples 1-4, in combination with Example 8, Example 5 and Comparative Example 3, it can be seen that when the mass ratio of Lysimachia sibiricum to sugar gradually increases, the relief time of the Chinese medicine composition gradually shortens, the degree of relief gradually increases, and the cumulative dissolution percentage gradually increases, but when the mass ratio of Lysimachia sibiricum to sugar reaches a certain value, the relief time of the Chinese medicine composition gradually prolongs, the degree of relief gradually decreases, and the cumulative dissolution percentage gradually decreases; Glycosylation reaction can significantly improve the solubility of flavonoid glycosides in Centella asiatica. Flavonoid glycosides themselves are usually hydrophobic and have low solubility, but by combining with sugar molecules, they can form more hydrophilic glycosylation products, thereby improving their solubility in water. Glycosylation products are more easily absorbed by the gastrointestinal tract because they have better water solubility and hydrophilicity; glycosylation can reduce the first-pass effect of drugs in the liver, allowing more drugs to enter the blood circulation and improve bioavailability; it can protect flavonoid glycosides from external factors such as oxidation, light, and temperature, and extend the shelf life of drugs; it can achieve rapid release, allowing the drug to reach a higher blood concentration in a short time and quickly relieve symptoms; in addition, glycosylation can reduce the bitterness and bad taste of drugs and improve patient compliance.

[0053] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. The above embodiments and descriptions are only preferred examples of the present invention and are not intended to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A Chinese medicine composition for rapidly relieving renal colic, characterized in that: The invention comprises the following components: 10-30 parts by weight of Lysimachia chinensis nanoparticles, 6-16 parts by weight of Corydalis yanhusuo, 8-18 parts by weight of white peony root, 5-15 parts by weight of chicken's gizzard lining, 8-18 parts by weight of oriental rhizome, 5-10 parts by weight of Lygodium japonicum, 2-8 parts by weight of beta-cyclodextrin, 0.5-1.5 parts by weight of poloxamer surfactant, and 2-8 parts by weight of sodium carboxymethyl cellulose; The Lysimachia nanoparticles are prepared by glycosylation reaction of flavonoid glycosides in the Lysimachia with sugar, followed by emulsification into nanoparticles; the sugar is any one of glucose, galactose and arabinose.

2. The Chinese medicine composition for rapidly relieving renal colic according to claim 1, characterized in that: The preparation method of the Lysimachia sibiricum nanoparticles is as follows: S1.1, weigh the following components in parts by weight respectively: 6-15 parts by weight of Lysimachia sibiricum, 40-60 parts by weight of ethanol, 3-8 parts by weight of sugar, 0.5-2 parts by weight of citric acid catalyst, 20-40 parts by weight of ethyl acetate solvent, and 5-15 parts by weight of polyvinyl alcohol surfactant; S1.2, crush the Lysimachia chinensis with a grinder at a speed of 1000-3000 rpm for 3-5 min to obtain Lysimachia chinensis powder with a particle size of 80-120 mesh; The desmodium powder is dissolved in 50-70% ethanol, and heated under reflux at 60-80° C. for 2-3 hours to obtain an extract, and the extract is cooled to room temperature and filtered to obtain a filtrate; The filtrate is placed in a rotary evaporator for concentration to obtain flavonoid glycosides; S1.3, dissolving flavonoid glycosides and sugar in deionized water, stirring with a magnetic stirrer at 300-500 rpm for 15-30 min at 30-40°C to obtain a mixed solution; The citric acid catalyst is added to the mixed solution and stirred continuously at a speed of 200-300 rpm for 10-30 min. The glycosylation reaction is carried out at 50-60° C. for 6-24 h. S1.

4. After the reaction is completed, the reaction mixture is filtered; the filtrate is placed in a rotary evaporator at a temperature of 50-60°C and a rotation speed of 50-100 rpm to obtain flavonoid glycosides; The flavonoid glycosides were dissolved in ethyl acetate and the polyvinyl alcohol surfactant was dissolved in water; The two solutions were mixed and stirred vigorously, and placed in a fume hood to form a nanoparticle suspension with a particle size of 75-120 μm; S1.5, centrifuging the nanoparticle suspension at a rotation speed of 1000-2000 rpm for 10-20 min, washing the nanoparticles with deionized water; vacuum drying the nanoparticles to obtain Lysimachia sibiricum nanoparticles.

3. The Chinese medicine composition for rapidly relieving renal colic according to claim 2, characterized in that: The temperature of the rotary evaporator is 50-70°C, and the rotation speed is 50-100 rpm.

4. The Chinese medicine composition for rapidly relieving renal colic according to claim 2, characterized in that: In S1.4, the vigorous stirring is performed at a stirring speed of 800-1000 rpm at room temperature for 10-20 min.

5. The Chinese medicine composition for rapidly relieving renal colic according to claim 2, characterized in that: The vacuum drying temperature is set at 30-50°C, and the drying time is 12-24h.

6. A method for preparing a Chinese medicine composition for rapidly relieving renal colic, for preparing the Chinese medicine composition for rapidly relieving renal colic as claimed in any one of claims 1 to 5, characterized in that: The steps include: S1. Weigh the following components in parts by weight respectively: 10-30 parts by weight of Lysimachia cyrtonema nanoparticles, 6-16 parts by weight of Corydalis yanhusuo, 8-18 parts by weight of Radix Paeoniae Alba, 5-15 parts by weight of Gallus galli, 8-18 parts by weight of Rhizoma Alismatis, 5-10 parts by weight of Lygodium japonicum, 2-8 parts by weight of β-cyclodextrin, 0.5-1.5 parts by weight of poloxamer surfactant, and 2-8 parts by weight of sodium carboxymethyl cellulose; S2, grinding Corydalis yanhusuo, white peony root, chicken gizzard lining, oriental water chestnut, and Lygodium japonicum with a grinder to obtain a mixed powder; S3, β-cyclodextrin is dissolved in deionized water to form a 10-20% β-cyclodextrin solution; sodium carboxymethyl cellulose is dissolved in deionized water to form a 0.5-1% sodium carboxymethyl cellulose solution; Dispersing the Lysimachia sibiricum nanoparticles and the mixed powder in S2 in deionized water, adding poloxamer surfactant, and stirring until completely dissolved to form a suspension; S4, adding the suspension to the β-cyclodextrin solution, stirring at 30-40°C at a speed of 300-400 rpm for 15-30 min, and adjusting the pH value of the solution to 6.0-7.5 with 0.3-0.5 mol / L phosphate buffer; After stirring evenly, ultrasonic treatment is performed with an ultrasonic power of 100-300 W and an ultrasonic time of 5-15 min, and then the mixture is allowed to stand for 1-2 h to allow the drug to be fully included in the cavity of β-cyclodextrin; Then add it to the sodium carboxymethyl cellulose solution and continue to stir evenly to ensure that the drug and carrier material are fully mixed; S5, pouring the mixed liquid into a pre-cooled mold, cooling and solidifying at room temperature, and taking out the solidified product after the mixed liquid is completely solidified; The solidified material is crushed by a pulverizer, the pulverizer speed is set to 1000-3000 rpm, the time is continued for 10-15 minutes, and particles with a particle size of 60-80 μm are obtained; S6. Freeze-drying the particles to obtain a Chinese medicinal composition for rapidly relieving renal colic.

7. The method for preparing the Chinese medicine composition for rapidly relieving renal colic according to claim 6, characterized in that: In S2, the rotation speed of the pulverizer is set to 1000-3000 rpm, the time lasts for 5-8 minutes, and the particle size is 100-200 meshes.

8. The method for preparing the Chinese medicine composition for rapidly relieving renal colic according to claim 6, characterized in that: In S3, the stirring is performed by a homogenizer, the stirring speed is 8000-10000 rpm, and the stirring time is 10-15 min.

9. The method for preparing the Chinese medicine composition for rapidly relieving renal colic according to claim 6, characterized in that: In S4, the stirring speed is 400-500 rpm, and the stirring time is 30-40 min.

10. The method for preparing the Chinese medicine composition for rapidly relieving renal colic according to claim 6, characterized in that: The freeze drying temperature is set at -40--50°C, and the drying time is 6-12 hours.

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