Microorganism culture and storage device and culture and storage method

Abstract

The invention discloses a microorganism culture and storage device and a culture and storage method, and belongs to the technical field of microorganism culture. The microorganism culture and storage device comprises a box body, a clamping mechanism for clamping culture dishes is arranged in the box body, the clamping mechanism comprises a mounting plate, a plurality of clamping grooves for placing the culture dishes are formed in the mounting plate, and clamping structures are arranged on the side walls of the clamping grooves. A transmission structure for driving the clamping structure to act is arranged between the fixing plate and the mounting plate in the incubator; a stirring structure for stirring a culture solution and ventilating is arranged in the shell of the culture dish, and a power structure for driving the stirring structure to rotate is arranged in the box body. By adopting the microorganism culture and storage device and the culture and storage method, the problems that metabolic substances are difficult to detect and the culture dish is unstable to fix in the microorganism culture process by adopting the culture dish can be solved.

Description

Technical Field

[0001] The present invention relates to the technical field of microorganism cultivation, and in particular to a microorganism cultivation storage device and a cultivation storage method. Background Art

[0002] Microbial biosynthesis refers to the process in which microorganisms use simple organic or inorganic substances as raw materials and synthesize complex organic molecules or bioactive substances through a series of enzymatic reactions. Microorganisms convert simple precursors into complex products through specific metabolic pathways to obtain synthetic organisms. Microorganisms precisely control the biosynthesis process through various mechanisms such as gene expression regulation and enzyme activity regulation. Microbial biosynthesis has a wide range of applications in biotechnology, pharmaceuticals, food industry and other fields. Antibiotics, enzyme preparations, vitamins, etc. produced by microorganisms are widely used in the medical field. Organic acids, amino acids, enzyme preparations, etc. produced by microorganisms are used in food processing and preservation. Organic acids, biopolymers, etc. synthesized by microorganisms are used to produce various chemicals and materials. Microorganisms can be used in environmental governance fields such as wastewater treatment and soil remediation. Biofuels produced by microbial fermentation can replace traditional fossil fuels and reduce environmental pollution.

[0003] The biosynthesis of microorganisms is carried out during the cultivation of microorganisms, and the cultivation of microorganisms plays a relatively important role in the biosynthesis process. The existing cultivation of microorganisms is generally carried out in a culture dish. Since the culture dish is a closed simple box structure, it is not convenient to detect and analyze the metabolites in the cultivation process.

[0004] The existing patent CN202310904767.6 discloses a microbial incubator that integrates liquid exchange and sampling, including a box body, a number of triangular culture bottles are placed in sequence inside the box body, a sealed box door is rotatably connected to one side of the box body, a material bin is arranged on one side of the top of the box body, a gun head slide rail assembly is installed inside the box body below the material bin, a mobile gun head is slidably connected to the middle of the gun head slide rail assembly, and a number of elastic connecting pipes are connected to the side wall of the middle of the mobile gun head. By adopting a mobile gun head design and a gun head slide rail assembly, a multifunctional combination of injecting culture fluid, replacing culture fluid and sampling is realized. Compared with manual operation, the steps are simpler, and after the above operations are completed, the steps of cleaning the gun head can be repeated and performed at any time, which can reduce the occurrence of the mobile gun head being contaminated by the external environment, and can also avoid the contamination of the internal residual liquid to the inside of the incubator. Although the above patent can realize the replacement of culture fluid, it is only applicable to culture bottles with open structures, and cannot be applied to culture dishes with closed structures; and the culture bottle is directly placed in the box, and the fixing effect of the culture bottle is relatively poor. Therefore, it is necessary to develop a device suitable for culture dishes that is convenient for metabolite detection. Summary of the invention

[0005] The purpose of the present invention is to provide a microorganism culture storage device and a culture storage method to solve the problem that it is difficult to detect metabolites during microorganism culture using a culture dish and the culture dish is not fixed stably.

[0006] To achieve the above-mentioned purpose, the present invention provides a microorganism culture storage device, including an incubator, a culture dish for culturing microorganisms is placed inside the box body of the incubator, a clamping mechanism for clamping the culture dish is arranged inside the box body, the clamping mechanism includes a mounting plate, a plurality of slots for placing the culture dish are arranged on the mounting plate, a clamping structure is arranged on the side wall of the slot, a transmission structure for driving the clamping structure to move is arranged between the fixed plate and the mounting plate in the incubator; a stirring structure for stirring and ventilating the culture liquid is arranged inside the shell of the culture dish, and a power structure for driving the stirring structure to rotate is arranged inside the box body.

[0007] Preferably, the clamping structure includes an arc-shaped clamping plate, the middle part of the clamping plate is connected to the vertically arranged transmission plate through a connecting plate, a limiting hole with a limiting effect on the sliding of the transmission plate is provided on the mounting plate, the transmission plate slides along a horizontal line passing through the central axis of the slot, a connecting hole for the connecting plate to pass through is provided between the limiting hole and the slot, the connecting plate slides horizontally along the connecting hole, an avoidance groove connected to the connecting hole is provided on the side wall of the slot, and the card plate is located in the avoidance groove; a sliding structure for driving the transmission plate to slide is provided at the bottom of the mounting plate, and the sliding structure is connected to the transmission structure.

[0008] Preferably, the sliding structure includes a rotating plate, a rotating shaft in the center of the rotating plate is rotatably connected to the mounting plate, the rotating plate is connected to the rotating shaft through a supporting rod, an arc-shaped slide groove inclined outward is provided on the rotating plate, a fixing pin is provided at the bottom end of the transmission plate, the fixing pin is located in the slide groove and slides along the slide groove, a fixing frame is provided on the mounting plate, the fixing frame is located outside the rotating plate, and the fixing frame is rotatably connected to the rotating plate; the rotating plate located in the middle of the mounting plate is connected to the transmission structure; adjacent rotating plates are connected through a transmission assembly, the transmission assembly includes a driven gear, the driven gear is rotatably arranged on the mounting plate, the driven gear is located between the two rotating plates, teeth meshing with the driven gear are provided on the side wall of the rotating plate, adjacent rotating plates are transmitted through the driven gear, and a through hole for the driven gear to pass through is provided on the fixed frame.

[0009] Preferably, the transmission structure includes a driving gear, which is rotatably connected to the mounting plate, a first rack meshing with the driving gear is arranged on the fixed plate, an intermediate gear is arranged on the wheel axle of the driving gear, the intermediate gear meshes with the transmission gear arranged on the rotating shaft of the rotating plate, the wheel diameter of the intermediate gear is smaller than the wheel diameter of the driving gear, the wheel diameter of the transmission gear is larger than the wheel diameter of the intermediate gear, and the diameter of the rotating plate is larger than the wheel diameter of the transmission gear; a guide rail that guides the sliding of the mounting plate is arranged on the fixed plate, and a handle that is convenient for pulling the mounting plate out of the box is arranged at the end of the mounting plate.

[0010] Preferably, a locking structure is provided between the fixing plate and the mounting plate, and the locking structure includes several locking blocks arranged on the mounting plate, the locking block is arranged at one end of the mounting plate located inside the box body, a locking hole is provided on the locking block, a locking pin adapted for the locking hole is slidably provided on the fixing plate, a sliding block is provided at the bottom of the locking pin, a mounting slot is provided inside the fixing plate, the sliding block is located in the mounting slot and is slidably connected to the mounting slot up and down, a through hole is provided on the mounting slot for the locking pin to pass through, a first spring is provided between the bottom of the mounting slot and the sliding block for applying an upward thrust to the locking pin; an inclined transmission hole is provided on the sliding block, a transmission pin is provided on a push rod on one side of the sliding block, the transmission pin is located in the transmission hole and is slidably connected to the transmission hole, a sliding hole for the push rod to pass through is provided on the fixing plate, the sliding hole is connected to the mounting slot and is vertically arranged, a push plate is provided at the end of the push rod, and a second spring is provided between the push plate and the fixing plate for applying an outward thrust to the push rod.

[0011] Preferably, the stirring structure includes a stirring assembly and a ventilation assembly, and the stirring assembly is coaxially arranged with the ventilation assembly; the ventilation assembly includes a central tube, which is located at the internal center of the stirring assembly and is rotatably connected to the stirring assembly, an air intake cavity is arranged inside the central tube, the top of the central tube is connected to an external air intake hose through a rotating joint, and a plurality of air intake main pipes are arranged at the bottom end of the central tube, and air intake branch pipes are arranged on both sides of the air intake main pipe, and the air intake branch pipes are connected to the air intake cavity through the air intake main pipe.

[0012] Preferably, the stirring assembly includes an outer tube, which is sleeved on the outside of the central tube, and the outer tube is rotatably connected to the central tube and the shell. A partition is provided inside the outer tube, and the partition divides the internal cavity of the outer tube into a closed liquid cavity and an exhaust cavity. A stirring rod is provided at the bottom of the outer tube, and a liquid inlet is provided on the stirring rod for allowing liquid to enter the internal liquid cavity of the outer tube. An exhaust port is provided on the side wall of the outer tube, and the exhaust port is connected to the exhaust cavity. The exhaust port is located at the upper part of the shell for discharging metabolic gases; a fixed cover is provided on the top of the shell, and the fixed cover is located outside the outer tube and is rotatably connected to the outer tube in a sealing manner. A baffle is provided inside the fixed cover to divide the fixed cover into two closed cavities, the upper closed cavity of the fixed cover is connected to the liquid cavity through the liquid outlet, and the lower closed cavity of the fixed cover is connected to the exhaust cavity through the air outlet. The discharge pipe provided on the upper closed cavity of the fixed cover is connected to an external liquid pump, and the exhaust pipe provided on the lower closed cavity of the fixed cover is connected to an external air pump.

[0013] Preferably, the power structure includes a skateboard, which is located above the shell, a screw rod is provided on the box body to drive the skateboard to slide along the length direction of the box body, a motor is provided on the box body to drive the screw rod to rotate, a guide rod is provided on the box body to guide the sliding of the skateboard, a second rack is provided on the skateboard, a third gear meshing with the second rack is provided on the outside of the top end of the center tube, a first gear is provided on the center tube, the first gear is meshed with a gear ring provided on the inner wall of the outer tube through the second gear, and the second gear is rotatably provided on the fixed cover.

[0014] Preferably, the box is provided with an insulation layer, one end of the box is provided with an insulation door that closes the box, the inside of the box is provided with a heating element and a lighting element, the heating element and the lighting element are electrically connected to the controller on the box, and the control panel on the box is electrically connected to the controller.

[0015] The culture and storage method based on the above-mentioned microorganism culture and storage device comprises the following steps:

[0016] S1. Open the door, press the push plate, the second spring is compressed, the push plate drives the push rod to slide inward, the push rod pushes the slider to slide downward along the installation slot through the transmission pin and the transmission hole, the first spring is compressed, the slider drives the locking pin to slide downward synchronously, and the locking pin slides out of the locking hole;

[0017] S2. Pull the mounting plate outward, the mounting plate slides outward along the guide rail, the driving gear on the mounting plate rotates under the action of the first rack of the fixed plate, the driving gear drives the transmission gear to rotate through the intermediate gear, the transmission gear drives the rotating plate to rotate, the rotating plate drives the adjacent rotating plates to rotate synchronously through the driven gear, the rotating plate drives the transmission plate to slide horizontally along the limiting hole through the sliding groove and the fixing pin, the transmission plate drives the clamping plate to slide toward the side wall of the card slot through the connecting plate, after the mounting plate is pulled out of the box, the clamping plate slides into the avoidance groove;

[0018] S3, placing the shell containing the culture solution and the inoculated microorganisms into the card slot, inserting the bottom plate into the card slot, connecting the top of the central tube to the air inlet hose through a rotary joint, connecting the drain pipe to the liquid pump through a hose, and connecting the exhaust pipe to the air pump through a hose;

[0019] S4, push the mounting plate inward, the driving gear rotates in the opposite direction under the action of the first rack, the clamping plate is clamped on the outer wall of the shell, and the bottom end of the clamping plate is against the top of the bottom plate; the locking pin slides into the locking hole along the inclined surface of the locking block to lock the mounting plate and the fixing plate; the third gear is in contact and meshing with the second rack;

[0020] S5, close the box door, set the temperature and light in the box, and carry out cultivation; start the motor, the motor drives the slide plate to slide through the screw rod, the slide plate drives the second rack to slide, the second rack drives the central tube to rotate through the third gear, the central tube drives the air intake manifold at the bottom to rotate, the central tube drives the gear ring to rotate through the first gear and the second gear, the gear ring drives the outer tube to rotate, the outer tube drives the stirring rod to rotate, and the culture solution is stirred;

[0021] S6. External gas enters the air inlet cavity through the rotary joint, and then enters the culture medium through the air inlet main pipe and the air inlet branch pipe to provide gas for the culture of microorganisms; under the action of the liquid pump, the culture medium enters the liquid cavity through the liquid inlet, enters the closed cavity at the upper part of the fixed cover through the liquid outlet, and is discharged through the drain pipe for metabolic substance detection; the waste gas generated by the metabolism of the shell enters the exhaust cavity through the exhaust port under the action of the air pump, enters the closed cavity at the lower part of the fixed cover through the air outlet, and is discharged through the exhaust pipe.

[0022] The advantages and positive effects of the microorganism culture storage device and culture storage method of the present invention are:

[0023] 1. The present invention is provided with a clamping mechanism inside the box, and the culture dish is clamped by the clamping structure on the clamping structure, thereby improving the stability of the culture dish. The rotating plates of adjacent clamping structures are connected by driven gears, so that the rotating plates rotate synchronously, and the clamping plates synchronously clamp the culture dish, which is convenient for the clamping operation of the culture dish.

[0024] 2. The present invention provides a transmission structure between the mounting plate and the fixed plate. When the mounting plate is pulled out of the box, the rotating plate is driven to rotate under the action of the transmission structure, thereby realizing the sliding of the clamping plate in a direction away from the culture dish; when the mounting plate is slid into the box, under the action of the transmission structure and the sliding structure, the mounting plate clamps the culture dish, realizing the clamping and fixing of the culture dish, and the operation is convenient.

[0025] 3. The present invention provides a locking structure between the mounting plate and the fixed plate, which improves the stability of the connection between the mounting plate and the fixed plate, and positions the mounting plate to improve the stability of the mounting plate position; by pressing the push plate, the push rod drives the slider and the locking pin to slide downward under the action of the transmission hole and the transmission pin, so as to facilitate the separation of the fixed plate from the mounting plate. The locking and separation operations between the mounting plate and the fixed plate are convenient.

[0026] 4. The present invention sets a ventilation component so that external oxygen or gas required for microbial reproduction enters the air intake cavity through the air intake hose and the rotary joint, and then enters the culture medium through the air intake main pipe and the air intake branch pipe, thereby increasing the amount of gas dissolved in the culture medium, which is beneficial to increasing the reproduction rate of microorganisms.

[0027] 5. The present invention provides a stirring assembly to pump out the culture solution through a liquid pump, which is convenient for testing the culture solution; and the metabolic gas generated by microbial reproduction can be discharged from the culture dish, reducing the inhibitory effect of metabolic gas on microbial reproduction, which is beneficial to increasing the reproduction rate of microorganisms. The stirring rod stirs the culture solution, which is beneficial to improving the uniformity of microbial distribution and the uniformity of oxygen distribution, etc., and is beneficial to increasing the reproduction rate.

[0028] 6. The present invention has a power structure inside the box body that drives the stirring structure to rotate. The power structure drives the central tube to rotate. The central tube drives the outer tube to rotate in the opposite direction through the first gear, the second gear and the gear ring, thereby providing a stirring effect on the culture solution.

[0029] The technical solution of the present invention is further described in detail below through the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 It is a schematic diagram of the three-dimensional structure of an embodiment of the present invention;

[0031] Figure 2 It is a front view structural schematic diagram of an embodiment of the present invention;

[0032] Figure 3 It is a schematic diagram of the front three-dimensional structure of the clamping mechanism according to an embodiment of the present invention;

[0033] Figure 4 It is a schematic diagram of the three-dimensional structure of the back side of the clamping mechanism according to an embodiment of the present invention;

[0034] Figure 5 It is a schematic diagram of the three-dimensional structure of the transmission structure of an embodiment of the present invention;

[0035] Figure 6 It is a front view structural schematic diagram of the transmission structure of an embodiment of the present invention;

[0036] Figure 7 It is a schematic diagram of the cross-sectional structure of the clamping structure according to an embodiment of the present invention;

[0037] Figure 8 It is a schematic diagram of the locking structure of an embodiment of the present invention;

[0038] Fig. 9 This is a schematic diagram of the three-dimensional structure of a culture dish according to an embodiment of the present invention;

[0039] Fig.10 This is a schematic diagram of the cross-sectional structure of a culture dish according to an embodiment of the present invention;

[0040] Fig.11 For attachment Fig.10 Middle A: Enlarged view;

[0041] Fig.12For attachment Fig.10 Middle B is an enlarged view.

[0042] Reference numerals

[0043] 1. Incubator; 11. Box; 12. Fixing plate; 13. Guide rail; 14. First rack; 15. Slide plate; 16. Second rack; 17. Screw rod; 18. Guide rod; 19. Motor; 110. Locking pin; 111. Sliding block; 112. Mounting slot; 113. Transmission hole; 114. Transmission pin; 115. First spring; 116. Second spring; 117. Push rod; 118. Push plate;

[0044] 2. Clamping mechanism; 21. Mounting plate; 22. Slot; 23. Clamping plate; 24. Connecting plate; 25. Transmission plate; 26. Fixing pin; 27. Limiting hole; 28. Avoiding groove; 29. ​​Rotating plate; 210. Slide; 211. Fixed frame; 212. Driving gear; 213. Intermediate gear; 214. Transmission gear; 215. Support rod; 216. Driven gear; 217. Locking block; 218. Locking hole;

[0045] 3. Culture dish; 31. Shell; 32. Bottom plate; 33. Center tube; 34. Outer tube; 35. Air intake main pipe; 36. Air intake branch pipe; 37. Air intake chamber; 38. Stirring rod; 39. Liquid inlet; 310. Liquid chamber; 311. Partition plate; 312. Exhaust port; 313. Exhaust chamber; 314. Fixed cover; 315. Baffle; 316. Liquid outlet; 317. Air outlet; 318. Drain pipe; 319. Exhaust pipe; 320. First gear; 321. Second gear; 322. Ring gear; 323. Third gear. DETAILED DESCRIPTION

[0046] In the description of the present invention, it should be noted that the terms "upper", "lower", "inside", "outside", etc. indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, or the positions or positional relationships in which the invented product is usually placed when in use. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention. In the description of the present invention, it should also be noted that, unless otherwise clearly specified and limited, the terms "setting", "installation", and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be a connection between the two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0047] The embodiments of the present invention are described in detail below in conjunction with the accompanying drawings.

[0048] like Figure 1 , Figure 2 As shown. A microorganism culture storage device includes an incubator 1, which is a rectangular box 11. The front end of the incubator 1 is provided with an opening for placing a culture dish 3, and a door is hinged at the opening. An insulation layer is provided on the box 11, and the door is an insulated door to improve the insulation effect of the box 11. A heating element and a lighting element are provided inside the box 11. The heating element can be a heating wire, a heating rod or a heating lamp to provide a heat source for the cultivation of microorganisms. The lighting element is a lighting lamp. A temperature sensor and a light intensity sensor are provided in the box 11. The temperature sensor and the light intensity sensor are electrically connected to the controller on the box 11 using existing technology as needed, and are used to control the temperature and light conditions in the box 11. The control panel on the box 11 is electrically connected to the controller using existing technology as needed, and the control panel is used to display parameters such as the temperature in the box 11 and to set the parameters. A battery can be provided in the box as needed or it can be directly connected to the mains through a plug. The fixed plate 12 in the box 11 can also be set as an insulation plate to divide the box 11 into several independent culture spaces. Different temperatures and lighting can be set for different culture spaces through heating elements and lighting elements, so as to study the effects of different culture environments on microbial culture.

[0049] like Figure 3 , Figure 4 , Figure 7 As shown. A clamping mechanism 2 for clamping the culture dish 3 is arranged inside the box body 11. The clamping mechanism 2 includes a mounting plate 21, and a plurality of slots 22 for placing the culture dish 3 are arranged on the mounting plate 21, and the diameter of the slot 22 is slightly larger than the diameter of the bottom plate 32 of the culture dish 3. A clamping structure is arranged on the side wall of the slot 22. The clamping structure includes an arc-shaped clamping plate 23 for clamping on the outer wall of the shell 31. The middle part of the clamping plate 23 is fixedly connected to the vertically arranged transmission plate 25 through a connecting plate 24. A limiting hole 27 with a limiting effect on the sliding of the transmission plate 25 is arranged on the mounting plate 21, and the width of the limiting hole 27 is slightly larger than the width of the transmission plate 25, so that the transmission plate 25 slides along the horizontal line passing the central axis of the slot 22, and cannot slide or rotate in other directions, thereby ensuring that the clamping plate 23 slides along the horizontal line passing the central axis of the slot 22. A connecting hole for the connecting plate 24 to pass through is provided between the limiting hole 27 and the card slot 22, and the connecting plate 24 slides horizontally along the connecting hole. An avoidance groove 28 connected to the connecting hole is provided on the side wall of the card slot 22, and the card plate is located in the avoidance groove 28 to prevent the card plate from affecting the placement of the culture dish 3 into the card slot 22.

[0050] like Figure 5 , Figure 6As shown. A sliding structure for driving the transmission plate 25 to slide is provided at the bottom of the mounting plate 21, and the sliding structure is connected to the transmission structure. The sliding structure includes a rotating plate 29, and the rotating shaft at the center of the rotating plate 29 is rotatably connected to the mounting plate 21 through a bearing. The rotating plate 29 is fixedly connected to the rotating shaft through a support rod 215. An arc-shaped slide groove 210 inclined outward is provided on the rotating plate 29, and a fixing pin 26 is fixedly provided at the bottom end of the transmission plate 25, and the fixing pin 26 is located in the slide groove 210 and slides along the slide groove 210. The rotating plate 29 drives the transmission plate 25 to slide horizontally through the slide groove 210 and the fixing pin 26. A fixed frame 211 is fixedly provided on the mounting plate 21, and the fixed frame 211 is located outside the rotating plate 29. The fixed frame 211 is rotatably connected to the rotating plate 29 through a bearing. The rotating plate 29 located in the middle of the mounting plate 21 is connected to the transmission structure.

[0051] Adjacent rotating plates 29 are connected by a transmission assembly. The transmission assembly includes a driven gear 216, which is rotatably arranged on the mounting plate 21 through a bearing. The driven gear 216 is located between two rotating plates 29. The side wall of the rotating plate 29 is provided with teeth meshing with the driven gear 216, and adjacent rotating plates 29 are transmitted through the driven gear 216. The fixed frame 211 is provided with a through hole for the driven gear 216 to pass through. The rotating plates 29 are meshed by the driven gear 216, so that the rotating plates 29 rotate synchronously, and the clamping plate 23 is synchronously clamped on the culture dish 3.

[0052] A transmission structure for driving the clamping structure is arranged between the fixed plate 12 and the mounting plate 21 in the incubator 1. The transmission structure includes a driving gear 212, and the driving gear 212 is rotatably connected to the mounting plate 21 through a bearing. A first rack 14 meshing with the driving gear 212 is fixedly arranged on the fixed plate 12, and the first rack 14 is arranged along the sliding direction of the mounting plate 21. An intermediate gear 213 is fixedly arranged on the wheel shaft of the driving gear 212, and the intermediate gear 213 meshes with a transmission gear 214 fixedly arranged on the rotating shaft of the rotating plate 29. The wheel diameter of the intermediate gear 213 is smaller than the wheel diameter of the driving gear 212, and the wheel diameter of the transmission gear 214 is larger than the wheel diameter of the intermediate gear 213. The diameter of the rotating plate 29 is larger than the wheel diameter of the transmission gear 214, so that the rotating plate 29 can rotate at a reduced speed. A guide rail 13 having a guiding effect on the sliding of the mounting plate 21 is fixedly arranged on the fixed plate 12. A handle is arranged at the end of the mounting plate 21 to facilitate pulling the mounting plate 21 out of the box body 11.

[0053] When the mounting plate 21 is pulled out from the box body 11, the rotating plate 29 is driven to rotate under the action of the transmission structure, so that the clamping plate 23 slides in the direction away from the culture dish 3; when the mounting plate 21 is slid into the box body 11, the mounting plate 21 clamps the culture dish 3 under the action of the transmission structure and the sliding structure, thereby clamping and fixing the culture dish 3, which is easy to operate.

[0054] like Figure 8 As shown. A locking structure is provided between the fixed plate 12 and the mounting plate 21. The locking structure includes a plurality of locking blocks 217 provided on the mounting plate 21, and the locking block 217 is fixedly provided at one end of the mounting plate 21 located inside the box body 11. A locking hole 218 is provided on the locking block 217, and a locking pin 110 adapted to the locking hole 218 is slidably provided on the fixed plate 12. An inclined surface is provided on the locking block 217 to facilitate the locking pin 110 to slide into the locking hole 218. A slider 111 is fixedly provided at the bottom of the locking pin 110, and a mounting groove 112 is provided inside the fixed plate 12. The slider 111 is located in the mounting groove 112 and is slidably connected to the mounting groove 112 up and down. A through hole is provided on the mounting groove 112 for the locking pin 110 to pass through, and the locking pin 110 is slidably connected to the through hole, and the locking pin 110 can only slide up and down under the action of the through hole, and cannot slide or rotate horizontally. A first spring 115 is disposed between the bottom of the mounting groove 112 and the sliding block 111 to apply an upward thrust to the locking pin 110 .

[0055] The slider 111 is provided with an inclined transmission hole 113, and a transmission pin 114 is fixedly provided on a push rod 117 on one side of the slider 111. The transmission pin 114 is located in the transmission hole 113 and is slidably connected to the transmission hole 113. The push rod 117 is provided in a one-to-one correspondence with the locking pin 110. The fixed plate 12 is provided with a sliding hole for the push rod 117 to pass through, and the sliding hole is connected to the mounting groove 112 and is vertically arranged. A push plate 118 is fixedly provided at the end of the push rod 117, and the push plate 118 fixes and connects the push rod 117 together. A second spring 116 is provided between the push plate 118 and the fixed plate 12 to apply an outward thrust to the push rod 117.

[0056] The locking structure improves the stability of the connection between the mounting plate 21 and the fixing plate 12, and positions the mounting plate 21 to improve the stability of the position of the mounting plate 21; by pressing the push plate 118, the push rod 117 drives the slider 111 and the locking pin 110 to slide downward under the action of the transmission hole 113 and the transmission pin 114, so as to facilitate the separation of the fixing plate 12 from the mounting plate 21. The locking and separation operations between the mounting plate 21 and the fixing plate 12 are convenient.

[0057] like Fig. 9 , Fig.10 , Fig.11 , Fig.12As shown. A stirring structure for stirring and ventilating the culture solution is arranged inside the shell 31 of the culture dish 3. The stirring structure includes a stirring assembly and a ventilation assembly, and the stirring assembly and the ventilation assembly are coaxially arranged. The ventilation assembly includes a central tube 33, and the central tube 33 is located at the inner center of the stirring assembly and is rotatably connected to the stirring assembly through a bearing. An air inlet cavity 37 is arranged inside the central tube 33, and the top of the central tube 33 is connected to an external air inlet hose through a rotating joint. A plurality of air inlet main pipes 35 are arranged at the bottom end of the central tube 33, and a plurality of air inlet branch pipes 36 are arranged on both sides of the air inlet main pipe 35, and the air inlet branch pipes 36 are connected to the air inlet cavity 37 through the air inlet main pipe 35.

[0058] The stirring assembly includes an outer tube 34, which is sleeved on the outside of the central tube 33, and the outer tube 34 is rotatably connected to the central tube 33 and the shell 31 through bearings. A partition 311 is provided inside the outer tube 34, and the partition 311 divides the internal cavity of the outer tube 34 into a closed liquid cavity 310 and an exhaust cavity 313. A stirring rod 38 is provided at the bottom of the outer tube 34, and a liquid inlet 39 is provided on the stirring rod 38 for allowing liquid to enter the liquid cavity 310 inside the outer tube 34. The liquid inlet 39 is located below the liquid level of the culture solution. An exhaust port 312 is provided on the side wall of the outer tube 34, and the exhaust port 312 is connected to the exhaust cavity 313. The exhaust port 312 is located in the upper part of the shell 31 and above the culture solution, and is used to discharge metabolic gases to reduce the inhibitory effect of metabolic gases on microbial reproduction.

[0059] A fixed cover 314 is provided on the top of the housing 31. The fixed cover 314 is located outside the outer tube 34 and is connected to the outer tube 34 in a sealed rotation manner through a bearing and a sealing ring. A baffle 315 is fixedly provided inside the fixed cover 314 to divide the fixed cover 314 into two closed cavities. The upper closed cavity of the fixed cover 314 is connected to the liquid cavity 310 through the liquid outlet 316, and the liquid discharge pipe 318 fixedly provided on the upper closed cavity of the fixed cover 314 is connected to an external liquid pump. The lower closed cavity of the fixed cover 314 is connected to the exhaust cavity 313 through the air outlet 317, and the exhaust pipe 319 fixedly provided on the lower closed cavity of the fixed cover 314 is connected to an external air pump.

[0060] Solenoid valves are provided on the air inlet pipe 36, the liquid inlet 39 and the exhaust port 312 as required, and the solenoid valves are electrically connected to the controller using existing technology to control the opening or closing of the pipelines. A corresponding gas concentration sensor can be provided in the housing 31 as required to detect the oxygen or metabolic gas concentration in the housing 31, so as to automatically add oxygen to the housing 31 or discharge metabolic gas.

[0061] By setting up a ventilation component, external oxygen or gas required for microbial reproduction enters the air intake chamber 37 through the air intake hose and the rotary joint, and then enters the culture medium through the air intake main pipe 35 and the air intake branch pipe 36, thereby increasing the amount of gas dissolved in the culture medium, which is beneficial to increasing the reproduction rate of microorganisms.

[0062] By setting a stirring assembly, the culture liquid is pumped out through a liquid pump, which is convenient for testing the culture liquid; and the metabolic gas generated by the microbial reproduction can be discharged from the culture dish 3, reducing the inhibitory effect of the metabolic gas on the microbial reproduction, which is beneficial to increasing the reproduction rate of the microorganisms. The stirring rod 38 stirs the culture liquid, which is beneficial to improving the uniformity of the distribution of microorganisms and the uniformity of the distribution of oxygen, etc., and is beneficial to increasing the reproduction rate.

[0063] The liquid pump and the air pump are electrically connected to the controller using existing technology as required.

[0064] The box 11 is provided with a power structure for driving the stirring structure to rotate. The power structure includes a slide plate 15, which is located above the housing 31. The box 11 is provided with a screw rod 17 for driving the slide plate 15 to slide along the length direction of the box 11. The screw rod 17 is rotatably connected to the box 11 through a bearing, and the screw rod 17 is threadedly connected to the slide plate 15. A motor 19 for driving the screw rod 17 to rotate is fixedly provided on the outside of the box 11. The motor 19 is electrically connected to the controller using existing technology as needed. A guide rod 18 for guiding the sliding of the slide plate 15 is fixedly provided on the box 11.

[0065] The slide plate 15 is fixedly provided with a second rack 16, and the top of the central tube 33 is fixedly provided with a third gear 323 meshing with the second rack 16. The central tube 33 is fixedly provided with a first gear 320, and the first gear 320 meshes with a gear ring 322 fixedly provided on the inner wall of the outer tube 34 through a second gear 321. The second gear 321 is rotatably provided on the fixed cover 314 through a bearing. The central tube 33 drives the outer tube 34 to rotate in the opposite direction through the first gear 320, the second gear 321 and the gear ring 322, thereby providing a stirring effect on the culture solution.

[0066] The culture and storage method based on the above-mentioned microorganism culture and storage device comprises the following steps:

[0067] S1. Open the door, press the push plate 118, the second spring 116 is compressed, the push plate 118 drives the push rod 117 to slide inward, the push rod 117 pushes the slider 111 to slide downward along the mounting groove 112 through the transmission pin 114 and the transmission hole 113, the first spring 115 is compressed, the slider 111 drives the locking pin 110 to slide downward synchronously, and the locking pin 110 slides out of the locking hole 218.

[0068] S2. Pull the mounting plate 21 outward, and the mounting plate 21 slides outward along the guide rail 13. The driving gear 212 on the mounting plate 21 rotates under the action of the first rack 14 of the fixed plate 12. The driving gear 212 drives the transmission gear 214 to rotate through the intermediate gear 213. The transmission gear 214 drives the rotating plate 29 to rotate. The rotating plate 29 drives the adjacent rotating plates 29 to rotate synchronously through the driven gear 216. The rotating plate 29 drives the transmission plate 25 to slide horizontally along the limiting hole 27 through the sliding groove 210 and the fixing pin 26. The transmission plate 25 drives the clamping plate 23 to slide toward the side wall of the slot 22 through the connecting plate 24. After the mounting plate 21 is pulled out of the box body 11, the clamping plate 23 slides into the avoidance groove 28.

[0069] S3. Place the housing 31 with the culture solution and the inoculated microorganisms into the slot 22, and insert the bottom plate 32 into the slot 22. Connect the top of the central tube 33 to the air inlet hose through a rotary joint, connect the drain pipe 318 to the liquid pump through a hose, and connect the exhaust pipe 319 to the air pump through a hose. The hose meets the need for length change when the mounting plate 21 slides.

[0070] S4, the mounting plate 21 is pushed inward, the driving gear 212 rotates in the opposite direction under the action of the first rack 14, the clamping plate 23 is clamped on the outer wall of the housing 31, and the bottom end of the clamping plate 23 abuts against the top of the bottom plate 32; the locking pin 110 slides into the locking hole 218 along the inclined surface of the locking block 217 to lock the mounting plate 21 and the fixing plate 12; the third gear 323 contacts and meshes with the second rack 16;

[0071] S5, close the box door, set the temperature and light in the box body 11, and start the culture. Start the motor 19, the motor 19 drives the slide plate 15 to slide through the screw rod 17, the slide plate 15 drives the second rack 16 to slide, the second rack 16 drives the central tube 33 to rotate through the third gear 323, the central tube 33 drives the air intake manifold 35 at the bottom thereof to rotate, the central tube 33 drives the gear ring 322 to rotate through the first gear 320 and the second gear 321, the gear ring 322 drives the outer tube 34 to rotate, the outer tube 34 drives the stirring rod 38 to rotate, and the culture solution is stirred.

[0072] S6. External gas enters the air inlet cavity 37 through the rotary joint, and then enters the culture liquid through the air inlet main pipe 35 and the air inlet branch pipe 36 to provide gas for the culture of microorganisms. Under the action of the liquid pump, the culture liquid enters the liquid cavity 310 through the liquid inlet 39, enters the upper closed cavity of the fixed cover 314 through the liquid outlet 316, and is then discharged through the liquid discharge pipe 318 for metabolic substance detection. The waste gas generated by the metabolism of the shell 31 enters the exhaust cavity 313 through the exhaust port 312 under the action of the air pump, enters the lower closed cavity of the fixed cover 314 through the air outlet 317, and is then discharged through the exhaust pipe 319.

[0073] Therefore, the microorganism culture storage device and culture storage method described in the present invention can solve the problem that it is difficult to detect metabolites during microorganism culture using a culture dish and the culture dish is unstable in fixation.

[0074] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. A microorganism culture storage device, comprising an incubator, wherein a culture dish for culturing microorganisms is placed inside the incubator, characterized in that: A clamping mechanism for clamping the culture dish is arranged inside the box body, and the clamping mechanism includes a mounting plate, on which a plurality of slots for placing the culture dish are arranged, and a clamping structure is arranged on the side wall of the slot, and a transmission structure for driving the clamping structure to move is arranged between the fixed plate and the mounting plate in the incubator; a stirring structure for stirring and ventilating the culture liquid is arranged inside the shell of the culture dish, and a power structure for driving the stirring structure to rotate is arranged inside the box body.

2. A microorganism culture storage device according to claim 1, characterized in that: The clamping structure includes an arc-shaped clamping plate, the middle part of which is connected to a vertically arranged transmission plate through a connecting plate, a limiting hole with a limiting effect on the sliding of the transmission plate is provided on the mounting plate, the transmission plate slides along a horizontal line passing through the central axis of the card slot, a connecting hole for the connecting plate to pass through is provided between the limiting hole and the card slot, the connecting plate slides horizontally along the connecting hole, an avoidance groove connected to the connecting hole is provided on the side wall of the card slot, and the card plate is located in the avoidance groove; a sliding structure for driving the transmission plate to slide is provided at the bottom of the mounting plate, and the sliding structure is connected to the transmission structure.

3. A microorganism culture storage device according to claim 2, characterized in that: The sliding structure includes a rotating plate, a rotating shaft in the center of the rotating plate is rotatably connected to the mounting plate, the rotating plate is connected to the rotating shaft through a supporting rod, an arc-shaped slide groove inclined outward is provided on the rotating plate, a fixing pin is provided at the bottom end of the transmission plate, the fixing pin is located in the slide groove and slides along the slide groove, a fixing frame is provided on the mounting plate, the fixing frame is located outside the rotating plate, and the fixing frame is rotatably connected to the rotating plate; the rotating plate located in the middle of the mounting plate is connected to the transmission structure; adjacent rotating plates are connected through a transmission assembly, the transmission assembly includes a driven gear, the driven gear is rotatably arranged on the mounting plate, the driven gear is located between the two rotating plates, teeth meshing with the driven gear are provided on the side wall of the rotating plate, adjacent rotating plates are transmitted through the driven gear, and a through hole for the driven gear to pass through is provided on the fixing frame.

4. A microorganism culture storage device according to claim 3, characterized in that: The transmission structure includes a driving gear, which is rotatably connected to the mounting plate, a first rack meshing with the driving gear is arranged on the fixed plate, an intermediate gear is arranged on the wheel shaft of the driving gear, the intermediate gear meshes with the transmission gear arranged on the rotating shaft of the rotating plate, the wheel diameter of the intermediate gear is smaller than the wheel diameter of the driving gear, the wheel diameter of the transmission gear is larger than the wheel diameter of the intermediate gear, and the diameter of the rotating plate is larger than the wheel diameter of the transmission gear; a guide rail with a guiding effect on the sliding of the mounting plate is arranged on the fixed plate, and a handle is arranged at the end of the mounting plate for facilitating the pulling out of the mounting plate from the box.

5. A microorganism culture storage device according to claim 4, characterized in that: The locking structure is provided between the fixing plate and the mounting plate, and the locking structure includes several locking blocks arranged on the mounting plate, the locking block is arranged at one end of the mounting plate located inside the box body, a locking hole is arranged on the locking block, a locking pin adapted for the locking hole is slidably provided on the fixing plate, a sliding block is provided at the bottom of the locking pin, a mounting slot is provided inside the fixing plate, the sliding block is located in the mounting slot and is slidably connected to the mounting slot up and down, a through hole is provided on the mounting slot for the locking pin to pass through, a first spring is provided between the bottom of the mounting slot and the sliding block for applying an upward thrust to the locking pin; an inclined transmission hole is provided on the sliding block, a transmission pin is provided on a push rod on one side of the sliding block, the transmission pin is located in the transmission hole and is slidably connected to the transmission hole, a sliding hole for the push rod to pass through is provided on the fixing plate, the sliding hole is connected to the mounting slot and is vertically arranged, a push plate is provided at the end of the push rod, and a second spring is provided between the push plate and the fixing plate for applying an outward thrust to the push rod.

6. A microorganism culture storage device according to claim 5, characterized in that: The stirring structure includes a stirring component and a ventilation component, and the stirring component and the ventilation component are coaxially arranged; the ventilation component includes a central tube, which is located at the internal center of the stirring component and is rotatably connected to the stirring component, an air intake cavity is arranged inside the central tube, and the top end of the central tube is connected to an external air intake hose through a rotating joint, and a plurality of air intake main pipes are arranged at the bottom end of the central tube, and air intake branch pipes are arranged on both sides of the air intake main pipe, and the air intake branch pipes are connected to the air intake cavity through the air intake main pipe.

7. A microorganism culture storage device according to claim 6, characterized in that: The stirring assembly includes an outer tube, which is sleeved on the outside of the central tube, and the outer tube is rotatably connected to the central tube and the shell. A partition is arranged inside the outer tube, and the partition divides the internal cavity of the outer tube into a closed liquid cavity and an exhaust cavity. A stirring rod is arranged at the bottom of the outer tube, and a liquid inlet is arranged on the stirring rod for allowing liquid to enter the internal liquid cavity of the outer tube. An exhaust port is arranged on the side wall of the outer tube, and the exhaust port is communicated with the exhaust cavity. The exhaust port is located at the upper part of the shell for discharging metabolic gas; a fixed cover is arranged on the top of the shell, and the fixed cover is located outside the outer tube and is rotatably connected to the outer tube. A baffle is arranged inside the fixed cover to divide the fixed cover into two closed cavities, the upper closed cavity of the fixed cover is communicated with the liquid cavity through the liquid outlet, and the lower closed cavity of the fixed cover is communicated with the exhaust cavity through the air outlet. The discharge pipe arranged on the upper closed cavity of the fixed cover is connected to an external liquid pump, and the exhaust pipe arranged on the lower closed cavity of the fixed cover is connected to an external air pump.

8. A microorganism culture storage device according to claim 7, characterized in that: The power structure includes a slide plate, which is located above the shell, a screw rod is provided on the shell body to drive the slide plate to slide along the length direction of the shell body, a motor is provided on the shell body to drive the screw rod to rotate, a guide rod is provided on the shell body to guide the sliding of the slide plate, a second rack is provided on the slide plate, a third gear meshing with the second rack is provided on the outside of the top end of the center tube, a first gear is provided on the center tube, the first gear is meshed with a gear ring provided on the inner wall of the outer tube through the second gear, and the second gear is rotatably provided on the fixed cover.

9. A microorganism culture storage device according to claim 8, characterized in that: The box body is provided with a heat-insulating layer, one end of the box body is provided with a heat-insulating door for closing the box body, a heating element and a lighting element are provided inside the box body, the heating element and the lighting element are electrically connected to the controller on the box body, and the control panel on the box body is electrically connected to the controller.

10. A cultivation and storage method for a microorganism cultivation and storage device according to claim 9, characterized in that: The following steps are involved: S1. Open the door, press the push plate, the second spring is compressed, the push plate drives the push rod to slide inward, the push rod pushes the slider to slide downward along the installation slot through the transmission pin and the transmission hole, the first spring is compressed, the slider drives the locking pin to slide downward synchronously, and the locking pin slides out of the locking hole; S2. Pull the mounting plate outward, the mounting plate slides outward along the guide rail, the driving gear on the mounting plate rotates under the action of the first rack of the fixed plate, the driving gear drives the transmission gear to rotate through the intermediate gear, the transmission gear drives the rotating plate to rotate, the rotating plate drives the adjacent rotating plates to rotate synchronously through the driven gear, the rotating plate drives the transmission plate to slide horizontally along the limiting hole through the sliding groove and the fixing pin, the transmission plate drives the clamping plate to slide toward the side wall of the card slot through the connecting plate, after the mounting plate is pulled out of the box, the clamping plate slides into the avoidance groove; S3, placing the shell containing the culture solution and the inoculated microorganisms into the card slot, inserting the bottom plate into the card slot, connecting the top of the central tube to the air inlet hose through a rotary joint, connecting the drain pipe to the liquid pump through a hose, and connecting the exhaust pipe to the air pump through a hose; S4, push the mounting plate inward, the driving gear rotates in the opposite direction under the action of the first rack, the clamping plate is clamped on the outer wall of the shell, and the bottom end of the clamping plate is against the top of the bottom plate; the locking pin slides into the locking hole along the inclined surface of the locking block to lock the mounting plate and the fixing plate; the third gear is in contact and meshing with the second rack; S5, close the box door, set the temperature and light in the box, and carry out cultivation; start the motor, the motor drives the slide plate to slide through the screw rod, the slide plate drives the second rack to slide, the second rack drives the central tube to rotate through the third gear, the central tube drives the air intake manifold at the bottom to rotate, the central tube drives the gear ring to rotate through the first gear and the second gear, the gear ring drives the outer tube to rotate, the outer tube drives the stirring rod to rotate, and the culture solution is stirred; S6. External gas enters the air inlet cavity through the rotary joint, and then enters the culture medium through the air inlet main pipe and the air inlet branch pipe to provide gas for the culture of microorganisms; under the action of the liquid pump, the culture medium enters the liquid cavity through the liquid inlet, enters the closed cavity at the upper part of the fixed cover through the liquid outlet, and is discharged through the drain pipe for metabolic substance detection; the waste gas generated by the metabolism of the shell enters the exhaust cavity through the exhaust port under the action of the air pump, enters the closed cavity at the lower part of the fixed cover through the air outlet, and is discharged through the exhaust pipe.

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