Fermentation strain for improving nutrition of animal feed and plant fertilizer and application thereof

Abstract

The invention provides a fermentative strain for improving nutrition of animal feed and plant fertilizer and application of the fermentative strain, and belongs to the technical field of biological fermentation. The plant fertilizer nutrition fermentation strain is prepared from, by mass, 1 part of acetic acid saccharomycetes, 1 part of lactic acid saccharomycetes, 1 part of bee fungi, brown sugar, 72% purified water and corn flour. The preparation method of the plant fertilizer nutrition fermentation strain comprises the steps of strain culture, mixing and activation and feed preparation. The nutritional value and the utilization efficiency of the feed and the fertilizer are improved, the digestion and absorption capacity of animals and the growth vigor of plants are enhanced, meanwhile, the use of chemical fertilizers is reduced, and the quality of the fertilizer is improved.

Description

Technical Field

[0001] The invention provides a fermentation strain for improving the nutrition of animal feed and plant fertilizer and application thereof, belonging to the technical field of biological fermentation. Background Art

[0002] In recent years, as people pay more attention to food safety and nutritional health, traditional feed and fertilizer can no longer meet the needs of modern agriculture and breeding. Existing feed and fertilizer often lack effective nutrients and have unsatisfactory effects on the growth of animals and plants. Therefore, developing an efficient and natural fermentation strain to improve the nutritional value of feed and fertilizer has become a problem to be solved.

[0003] Therefore, in view of this, the existing structure is studied and improved, and a fermentation strain for improving the nutrition of animal feed and plant fertilizer and its application are proposed to solve the above-mentioned problems. Summary of the invention

[0004] The present invention provides a fermentation strain for improving the nutrition of animal feed and plant fertilizer and its application, which not only improves the nutritional value and utilization efficiency of feed and fertilizer, but also enhances the digestion and absorption capacity of animals and the growth vitality of plants, while reducing the use of chemical fertilizers and improving the quality of fertilizers.

[0005] In order to solve the above problems, the present invention proposes a technical solution: a fermentation strain for improving the nutrition of animal feed and plant fertilizer and its application, wherein the plant fertilizer nutrition fermentation strain is composed of the following strains in mass ratio:

[0006] 1 part of acetic acid yeast,

[0007] 1 portion of lactic acid yeast,

[0008] 1 portion of bee fungus,

[0009] brown sugar,

[0010] 72% pure water,

[0011] Corn flour,

[0012] The steps of preparing the fertilizer nutrient fermentation bacteria include bacteria culture, mixing and activation, and feed preparation.

[0013] Furthermore, the bacterial strain culture is to add acetic acid yeast, lactic acid yeast and bee fungus to a suitable culture medium, culture at 37°C for 24 hours, separate the cultured bacterial liquid by centrifugation and collect the bacterial bodies.

[0014] Furthermore, the mixing and activation is to mix the collected bacteria in a certain proportion, add a proper amount of activation liquid, mix well, and activate for 12 hours.

[0015] Furthermore, the activation solution is composed of a carbon source, a nitrogen source, minerals, growth factors, and a buffer.

[0016] Further, the feed preparation steps are as follows:

[0017] S1. Mixing strains: Take 1 part of acetic acid yeast, 1 part of lactic acid yeast and 1 part of bee fungus respectively, mix the three strains in a small container, and stir gently to mix evenly;

[0018] S2. Dissolve brown sugar: Add 720 ml of pure water to a measuring cup, gradually add brown sugar into the pure water, and stir until the brown sugar is completely dissolved.

[0019] S3. Corn flour mixing: gradually add corn flour into the purified water dissolved with brown sugar, stirring at the same time, until the corn flour is completely mixed; finally, add the mixed bacteria into the solution of brown sugar and corn flour, stir gently to ensure that all ingredients are fully mixed.

[0020] S4. Perform anaerobic fermentation: Pour the mixed fermentation liquid into the prepared container, cover it with a lid to ensure good sealing, place it in a suitable fermentation environment at a temperature of about 25-30°C, and perform anaerobic fermentation for 48 hours.

[0021] S5. Addition of feed and fertilizer: Take out 5% of the fermentation product and add it to common feed and fertilizer; perform anaerobic fermentation for 48 hours, and add 30% of pure water, mix well and ferment.

[0022] Furthermore, the calculated amount of brown sugar in step S2 is that the total volume of the fermentation liquid is 1000 ml, brown sugar accounts for 10-15%, and the amount of brown sugar is 100-150 g.

[0023] Furthermore, the calculated amount of corn in step S3 is that the total fermentation liquid volume is 1000 ml, corn flour accounts for 13%, and the amount of corn flour is 130 g.

[0024] Due to the adoption of the above technical solution, the beneficial effects of the fermentation bacteria for improving the nutrition of animal feed and plant fertilizer and the application thereof of the present invention are:

[0025] 1. By adding acetic acid yeast, lactic acid yeast and bee fungus, these three strains can effectively decompose the complex organic matter in the feed and convert it into nutrients that are more easily absorbed by animals, such as amino acids, vitamins and minerals, thereby improving the nutritional value of the feed. This fermentation process can also increase the palatability of the feed, improve the appetite and digestion and absorption capacity of animals, thereby promoting the growth and health of animals.

[0026] 2. The use of fermentation bacteria can decompose large molecular organic substances in plant fertilizers into smaller molecules. These small molecules are more easily absorbed by plant roots, improving the utilization efficiency of fertilizers. The organic acids and active enzymes produced during the fermentation process can improve soil structure, increase soil fertility, promote plant growth, reduce the use of chemical fertilizers, and improve fertilizer quality. DETAILED DESCRIPTION

[0027] The technical solution will be described clearly and completely below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] The present invention provides a fermentation strain for improving the nutrition of animal feed and plant fertilizer and its application: the plant fertilizer nutrition fermentation strain is composed of the following strains in mass ratio:

[0029] 1 part of acetic acid yeast,

[0030] 1 portion of lactic acid yeast,

[0031] 1 portion of bee fungus,

[0032] brown sugar,

[0033] 72% pure water,

[0034] Corn flour,

[0035] The steps of preparing the fertilizer nutrient fermentation bacteria include bacteria culture, mixing and activation, and feed preparation.

[0036] The bacterial strain culture is to add acetic acid yeast, lactic acid yeast and bee fungus to a suitable culture medium, culture at 37°C for 24 hours, separate the cultured bacterial liquid by centrifugation, and collect the bacterial bodies.

[0037] The mixing and activation is to mix the collected bacteria in a certain proportion, add a proper amount of activation liquid, mix well, and activate for 12 hours.

[0038] The activation solution consists of a carbon source, a nitrogen source, minerals, growth factors and a buffer.

[0039] The feed preparation steps are as follows:

[0040] S1. Mixing strains: Take 1 part of acetic acid yeast, 1 part of lactic acid yeast and 1 part of bee fungus respectively, mix the three strains in a small container, and stir gently to mix evenly;

[0041] S2. Dissolve brown sugar: Add 720 ml of pure water to a measuring cup, gradually add brown sugar into the pure water, and stir until the brown sugar is completely dissolved.

[0042] S3. Corn flour mixing: gradually add corn flour into the purified water dissolved with brown sugar, stirring at the same time, until the corn flour is completely mixed; finally, add the mixed bacteria into the solution of brown sugar and corn flour, stir gently to ensure that all ingredients are fully mixed.

[0043] S4. Perform anaerobic fermentation: Pour the mixed fermentation liquid into the prepared container, cover it with a lid to ensure good sealing, place it in a suitable fermentation environment at a temperature of about 25-30°C, and perform anaerobic fermentation for 48 hours.

[0044] S5. Addition of feed and fertilizer: Take out 5% of the fermentation product and add it to common feed and fertilizer; perform anaerobic fermentation for 48 hours, and add 30% of pure water, mix well and ferment.

[0045] The calculated amount of brown sugar in step S2 is that the total volume of the fermentation liquid is 1000 ml, brown sugar accounts for 10-15%, and the amount of brown sugar is 100-150 g.

[0046] The calculated amount of corn in step S3 is that the total fermentation liquid volume is 1000 ml, corn flour accounts for 13%, and the amount of corn flour is 130 g.

[0047] Among them, adding 5% of this fermentation strain into pig feed, after 48 hours of anaerobic fermentation, the feed is full of fragrance, the pigs grow normally, the meat is bright red, the arachidonic acid in pork is increased by more than 63%, and the nucleic acid is increased by more than 57%; adding 5% of this fermentation strain into plant fertilizer, after 48 hours of anaerobic fermentation, the plants grow rapidly after application, the taste and color of the fruit are significantly improved, the arachidonic acid in plants (rice bran) is increased by more than 61%, the nucleic acid is increased by more than 53%, and at the same time, the trace elements such as magnesium, selenium, and californium in the product are greatly improved.

[0048] The present invention and its implementation methods are described above, and such description is not restrictive. In short, if a person skilled in the art is inspired by it and, without departing from the purpose of the invention, designs structures and embodiments similar to the technical solution without creativity, they should all fall within the protection scope of the present invention.

Claims

1. A fermentation strain for improving the nutrition of animal feed and plant fertilizer and its application, wherein the plant fertilizer nutrition fermentation strain is composed of the following strains in mass ratio: 1 part of acetic acid yeast, 1 portion of lactic acid yeast, 1 portion of bee fungus, brown sugar, 72% pure water, Corn flour, The steps of preparing the fertilizer nutrient fermentation bacteria include bacteria culture, mixing and activation, and feed preparation.

2. The fermentation bacteria for improving the nutrition of animal feed and plant fertilizer and its application according to claim 1, characterized in that: The bacterial strain culture is to add acetic acid yeast, lactic acid yeast and bee fungus to a suitable culture medium, culture at 37°C for 24 hours, separate the cultured bacterial liquid by centrifugation, and collect the bacterial bodies.

3. The fermentation bacteria for improving the nutrition of animal feed and plant fertilizer and its application according to claim 1, characterized in that: The mixing and activation is to mix the collected bacteria in a certain proportion, add a proper amount of activation liquid, mix well, and activate for 12 hours.

4. The fermentation bacteria for improving the nutrition of animal feed and plant fertilizer and its application according to claim 1, characterized in that: The activation solution consists of a carbon source, a nitrogen source, minerals, growth factors and a buffer.

5. According to claim 1, a fermentation strain for improving the nutrition of animal feed and plant fertilizer and its application, the feed preparation steps are as follows: S1. Mixing strains: Take 1 part of acetic acid yeast, 1 part of lactic acid yeast and 1 part of bee fungus respectively, mix the three strains in a small container, and stir gently to mix evenly; S2. Dissolve brown sugar: Add 720 ml of pure water to a measuring cup, gradually add brown sugar into the pure water, and stir until the brown sugar is completely dissolved. S3. Corn flour mixing: gradually add corn flour into the purified water dissolved with brown sugar, stirring at the same time, until the corn flour is completely mixed; finally, add the mixed bacteria into the solution of brown sugar and corn flour, stir gently to ensure that all ingredients are fully mixed. S4. Perform anaerobic fermentation: Pour the mixed fermentation liquid into the prepared container, cover it with a lid to ensure good sealing, place it in a suitable fermentation environment at a temperature of about 25-30°C, and perform anaerobic fermentation for 48 hours. S5. Addition of feed and fertilizer: Take out 5% of the fermentation product and add it to common feed and fertilizer; perform anaerobic fermentation for 48 hours, and add 30% of pure water, mix well and ferment.

6. The fermentation bacteria for improving the nutrition of animal feed and plant fertilizer and its application according to claim 5, characterized in that: The calculated amount of brown sugar in step S2 is that the total volume of the fermentation liquid is 1000 ml, brown sugar accounts for 10-15%, and the amount of brown sugar is 100-150 g.

7. The fermentation bacteria for improving the nutrition of animal feed and plant fertilizer and its application according to claim 5, characterized in that: The calculated amount of corn in step S3 is that the total fermentation liquid volume is 1000 ml, corn flour accounts for 13%, and the amount of corn flour is 130 g.