Light and thin sunscreen cream capable of rapidly forming film as well as preparation method and application of light and thin sunscreen cream

Abstract

The invention provides a light and thin sunscreen cream capable of rapidly forming a film as well as a preparation method and application thereof. The sunscreen cream is prepared from the following raw materials in parts by weight: 19 to 25 parts of apple essence, 13 to 17 parts of tremella sporocarp extract, 7 to 10 parts of glycyrrhiza glabra extract, 9 to 14 parts of cynanchum atratum extract, 1 to 4 parts of 4-potassium methoxysalicylate, 4 to 7 parts of hydroxypinacolato retinate, 5 to 8 parts of nicotinamide PC, 1 to 3 parts of 4-butylresorcinol, 4 to 8 parts of sunscreen agent, 2 to 4 parts of hydroxyethyl cellulose, 2 to 4 parts of butanediol, 1 to 3 parts of propylene glycol and 2 to 2 parts of glyceryl stearate. 4 parts of lecithin, 2-4 parts of lecithin, 0.01-0.05 part of preservative and 120-150 parts of water. The prepared sunscreen cream has a good using effect, can be easily pushed away in the using process to form a sunscreen protection film, is light, thin, non-sticky, capable of effectively resisting ultraviolet rays, light in skin feeling, free of burden, capable of improving the moisture degree of the skin and preventing the skin from sunburn and sunburn, capable of preventing the skin from sunburn and aging and good in acting effect.

Description

Technical Field

[0001] The invention relates to the technical field of sunscreen creams, and in particular to a light and thin sunscreen cream with fast film formation, and a preparation method and application thereof. Background Art

[0002] As people's living standards improve, more and more people pay more attention to their skin, and fair skin has always been the pursuit of women. The skin is the first physiological barrier between the body and the outside world. Under ultraviolet radiation, the skin will age and cause skin melanin deposition, making the skin darker. Therefore, in order to prevent the skin from darkening, whitening sunscreen is often chosen to block ultraviolet radiation and thus protect the skin. At present, common sunscreens are still mostly made of chemical synthetic raw materials, which are easy to irritate the skin, and due to the unreasonable formula of sunscreen raw materials, the whitening and sunscreen effect is not ideal. After use, it will appear very sticky, and the skin is also prone to dryness, which causes the skin to be damaged. In addition, the sunscreen effect is not good, and the skin is also prone to become dark and the spots increase. Summary of the invention

[0003] In view of this, the present invention proposes a light and thin sunscreen with fast film formation and a preparation method and application thereof. The sunscreen of the present invention is light and thin, non-sticky, easy to spread and form a film, mild and non-irritating, plays an effective sunscreen role, and has a moisturizing effect on the skin, preventing the skin from becoming dull and dark.

[0004] The technical solution of the present invention is achieved in this way:

[0005] A fast-film-forming light sunscreen comprises the following raw materials in parts by weight: 19-25 parts of apple essence, 13-17 parts of Tremella fuciformis fruiting body extract, 7-10 parts of Glycyrrhiza glabra extract, 9-14 parts of Psoralea corylifolia extract, 1-4 parts of 4-methoxysalicylic acid potassium salt, 4-7 parts of hydroxypinacolone retinoic acid ester, 5-8 parts of nicotinamide PC, 1-3 parts of 4-butylresorcinol, 4-8 parts of sunscreen, 2-4 parts of hydroxyethyl cellulose, 2-4 parts of butylene glycol, 1-3 parts of propylene glycol, 2-4 parts of glyceryl stearate, 2-4 parts of lecithin, 0.01-0.05 parts of preservative and 120-150 parts of water.

[0006] Furthermore, the fast-film-forming thin sunscreen comprises the following raw materials in parts by weight: 25 parts of apple extract, 13 parts of Tremella fuciformis fruiting body extract, 10 parts of Glycyrrhiza glabra extract, 12 parts of Psoralea corylifolia extract, 3 parts of 4-methoxysalicylic acid potassium salt, 7 parts of hydroxypinacolone retinoic acid ester, 7 parts of niacinamide PC, 1.5 parts of 4-butylresorcinol, 6 parts of sunscreen, 2.6 parts of hydroxyethyl cellulose, 2.3 parts of butylene glycol, 2.5 parts of propylene glycol, 3 parts of glyceryl stearate, 4 parts of lecithin, 0.01 parts of preservative, and 128 parts of water.

[0007] Furthermore, the sunscreen is one or both of titanium dioxide and zinc oxide.

[0008] Furthermore, the preservative is one or both of methylparaben and propylparaben.

[0009] Furthermore, the preparation method of the apple essence is as follows: fresh apples are washed, crushed and juiced, apple pomace is freeze-dried and then crushed to obtain apple pomace powder, the apple pomace powder is added to an aqueous solution containing 2-5% of Vc by mass, stirred and mixed, a composite enzyme is added to perform enzymolysis at 40-50° C. for 50-60 minutes, the pressure is maintained at 300-500 MPa for 5-7 minutes, filtered, and the filtrate is concentrated under reduced pressure to obtain the apple essence.

[0010] Furthermore, the ratio of apple pomace powder to aqueous solution is 1:15-18 g / mL; the mass of the added complex enzyme is 1-1.4% of the aqueous solution; the complex enzyme includes β-glucanase and pectinase in a mass ratio of 3-5:1-2.

[0011] The method for preparing the fast-film-forming thin sunscreen of the present invention comprises the following steps:

[0012] (1) adding butylene glycol and propylene glycol to half the amount of water and stirring once, then adding apple extract, Tremella fuciformis fruiting body extract, Glycyrrhiza glabra extract, Psoralea corylifolia extract, 4-methoxysalicylic acid potassium salt, and niacinamide PC and stirring twice to obtain a mixed material A;

[0013] (2) adding hydroxyethyl cellulose, glyceryl stearate, and lecithin to the other half of the water in the formula and stirring to mix, and then adding hydroxypinacolone retinoic acid ester, 4-butylresorcinol, and a sunscreen agent and stirring to mix to obtain a mixture B;

[0014] (3) Adding mixed material A and preservatives to mixed material B and stirring to obtain the target sunscreen.

[0015] Furthermore, in step (1), the first stirring speed is 150-180 r / min, and the second stirring speed is 300-400 r / min.

[0016] Furthermore, in step (2), the stirring speed is 200 to 300 r / min; in step (3), the stirring speed is 350 to 450 r / min.

[0017] Furthermore, the fast-film-forming, light and thin sunscreen of the present invention is used in the preparation of skin care products.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] The sunscreen prepared by the present invention is light, thin, non-greasy, and easy to spread on the skin surface to form a protective film through the synergistic effect of various raw materials by scientifically matching the sunscreen with apple essence, tremella fruiting body extract, iris oleifera extract, etc., and reduces the stimulation and damage of the external environment such as ultraviolet rays to the skin; through the synergistic effect of raw materials such as apple essence, tremella fruiting body extract, iris oleifera extract, glyceryl stearate, etc., the skin moisture can be locked, the skin can be kept hydrated, and dryness can be prevented, and the generation of melanin can be reduced, skin spots and dullness can be reduced, and the sunscreen whitening effect can be improved.

[0020] The present invention can inhibit the production of melanin through the synergistic effect of licorice extract, 4-methoxysalicylic acid potassium salt and hydroxypinacolone retinoic acid ester, and reduce the production of melanin by blocking tyrosinase activity through the action of nicotinamide PC, and can also prevent melanin transport, thereby reducing the deposition of melanin in the epidermis. In addition, nicotinamide PC can also synergize with Tremella fruiting body extract and 4-butylresorcinol to promote the metabolism of the stratum corneum, accelerate the shedding of keratinocytes containing melanin, and achieve the effect of post-sun freckle removal and whitening from the three dimensions of the whole link. In addition, the present invention adopts pure physical sunscreen, which can avoid the irritation and damage to the skin caused by chemical sunscreens, and has a good effect. The present invention extracts the active ingredients in apples through suitable enzymolysis and ultra-high pressure processes, and can obtain apple essence with excellent effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is the test result of moisture content in skin stratum corneum.

[0022] Figure 2 It is the result of transepidermal water loss test.

[0023] Figure 3 This is the result of skin melanin detection.

[0024] Figure 4 This is the detection result of skin spot area ratio. DETAILED DESCRIPTION

[0025] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0026] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0027] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0028] The Tremella fuciformis fruiting body extract of the invention is Tremella fuciformis fruiting body extract, the Glycyrrhiza glabra extract is Glycyrrhiza glabra extract, and the Cynanchum cynanchum extract is Cynanchum cynanchum extract.

[0029] The beta-glucanase of the present invention is purchased from Sichuan Chengzhu Biotechnology Co., Ltd. and is named beta-glucanase, and the enzyme activity is ≥50,000 U / g.

[0030] The pectinase of the present invention is purchased from Sichuan Chengzhu Biotechnology Co., Ltd. and is named pectinase, with an enzyme activity of ≥29,000 U / g.

[0031] Example 1

[0032] The preparation method of the apple essence of the present embodiment is as follows: fresh apples are washed, crushed and juiced, apple pomace is freeze-dried and then crushed to obtain apple pomace powder, the apple pomace powder is added to an aqueous solution containing 5% Vc by mass concentration according to a solid-liquid ratio of 1:16 (g / mL), and the mixture is stirred and evenly mixed, 1.3% of the mass of the aqueous solution of a complex enzyme consisting of β-glucanase and pectinase in a mass ratio of 4:1.5 is added, the mixture is hydrolyzed at 45° C. for 60 minutes, the mixture is kept under pressure at 400 MPa for 7 minutes, filtered, and the filtrate is concentrated under reduced pressure to obtain the apple essence;

[0033] The fast-film-forming thin sunscreen of the present embodiment comprises the following raw materials in parts by weight: 25 parts of apple extract, 13 parts of Tremella fuciformis fruiting body extract, 10 parts of Glycyrrhiza glabra extract, 12 parts of Psoralea corylifolia extract, 3 parts of 4-methoxysalicylic acid potassium salt, 7 parts of hydroxypinacolone retinoic acid ester, 7 parts of niacinamide PC, 1.5 parts of 4-butylresorcinol, 6 parts of sunscreen, 2.6 parts of hydroxyethyl cellulose, 2.3 parts of butylene glycol, 2.5 parts of propylene glycol, 3 parts of glyceryl stearate, 4 parts of lecithin, 0.01 parts of preservative, and 128 parts of water; the sunscreen is composed of titanium dioxide and zinc oxide in a mass ratio of 2:1; the preservative is methylparaben;

[0034] The method for preparing the fast-film-forming thin sunscreen of the present embodiment is characterized by comprising the following steps:

[0035] (1) adding butylene glycol and propylene glycol to half the amount of water in the formula and stirring at a stirring speed of 180 r / min for a first stirring and mixing, and then adding apple extract, Tremella fuciformis fruiting body extract, Glycyrrhiza glabra extract, Psoralea corylifolia extract, 4-methoxysalicylic acid potassium salt, and niacinamide PC and stirring at a stirring speed of 400 r / min for a second stirring and mixing to obtain a mixed material A;

[0036] (2) adding hydroxyethyl cellulose, glyceryl stearate, and lecithin to the other half of the water in the formula and stirring at a stirring speed of 200 r / min, and then adding hydroxypinacolone retinoic acid ester, 4-butylresorcinol, and a sunscreen agent and stirring at a stirring speed of 200 r / min to obtain a mixture B;

[0037] (3) Adding mixed material A and preservatives to mixed material B and stirring at a stirring speed of 400 r / min, a light and thin sunscreen with fast film formation is obtained.

[0038] Example 2

[0039] The preparation method of the apple essence of the present embodiment is as follows: fresh apples are washed, crushed and juiced, apple pomace is freeze-dried and then crushed to obtain apple pomace powder, the apple pomace powder is added to an aqueous solution containing 3% of Vc by mass at a solid-liquid ratio of 1:15 (g / mL), and the mixture is stirred and evenly mixed, 1% of the mass of the aqueous solution of a complex enzyme consisting of β-glucanase and pectinase in a mass ratio of 5:2 is added, and enzymolysis is performed at 50° C. for 50 minutes, and the pressure is maintained at 300 MPa for 6 minutes, and the filtrate is filtered, and the filtrate is concentrated under reduced pressure to obtain the apple essence;

[0040] The fast-film-forming thin sunscreen of this embodiment comprises the following raw materials in parts by weight: 19 parts of apple extract, 15 parts of Tremella fuciformis fruiting body extract, 7 parts of Glycyrrhiza glabra extract, 10 parts of Psoralea corylifolia extract, 4 parts of 4-methoxysalicylic acid potassium salt, 4 parts of hydroxypinacolone retinoic acid ester, 6 parts of niacinamide PC, 1.4 parts of 4-butylresorcinol, 5 parts of sunscreen, 3 parts of hydroxyethyl cellulose, 4 parts of butylene glycol, 2.5 parts of propylene glycol, 4 parts of glyceryl stearate, 2.6 parts of lecithin, 0.03 parts of preservative, and 136 parts of water; the sunscreen is titanium dioxide; the preservative is propylparaben;

[0041] The method for preparing the fast-film-forming thin sunscreen of the present embodiment is characterized by comprising the following steps:

[0042] (1) adding butylene glycol and propylene glycol to half the amount of water in the formula and stirring at a stirring speed of 160 r / min for a first stirring and mixing, and then adding apple extract, Tremella fuciformis fruiting body extract, Glycyrrhiza glabra extract, Psoralea corylifolia extract, 4-methoxysalicylic acid potassium salt, and niacinamide PC and stirring at a stirring speed of 300 r / min for a second stirring and mixing to obtain a mixed material A;

[0043] (2) adding hydroxyethyl cellulose, glyceryl stearate, and lecithin to the other half of the water in the formula and stirring at a stirring speed of 250 r / min, and then adding hydroxypinacolone retinoic acid ester, 4-butylresorcinol, and a sunscreen agent and stirring at a stirring speed of 250 r / min to obtain a mixture B;

[0044] (3) Adding mixed material A and preservatives to mixed material B and stirring at a stirring speed of 350 r / min, a light and thin sunscreen with fast film formation is obtained.

[0045] Example 3

[0046] The preparation method of the apple essence of the present embodiment is as follows: fresh apples are washed, crushed and juiced, apple pomace is freeze-dried and then crushed to obtain apple pomace powder, the apple pomace powder is added to an aqueous solution containing 2% Vc by mass concentration according to a solid-liquid ratio of 1:18 (g / mL), and the apple pomace powder is stirred and mixed, 1.2% of the mass of the aqueous solution is added with a complex enzyme consisting of β-glucanase and pectinase in a mass ratio of 3:1, and the mixture is hydrolyzed at 40° C. for 55 minutes, and the pressure is maintained at 500 MPa for 5 minutes, filtered, and the filtrate is concentrated under reduced pressure to obtain apple essence;

[0047] The fast-film-forming thin sunscreen of the present embodiment comprises the following raw materials in parts by weight: 22 parts of apple extract, 17 parts of Tremella fuciformis fruiting body extract, 9 parts of Glycyrrhiza glabra extract, 14 parts of Psoralea corylifolia extract, 1 part of 4-methoxysalicylic acid potassium salt, 6 parts of hydroxypinacolone retinoic acid ester, 5 parts of niacinamide PC, 1.3 parts of 4-butylresorcinol, 7 parts of sunscreen, 3 parts of hydroxyethyl cellulose, 3 parts of butylene glycol, 2 parts of propylene glycol, 3 parts of glyceryl stearate, 2.5 parts of lecithin, 0.02 parts of preservative, and 130 parts of water; the sunscreen is zinc oxide; the preservative is composed of methylparaben and propylparaben in a mass ratio of 1:2;

[0048] The method for preparing the fast-film-forming thin sunscreen of the present embodiment is characterized by comprising the following steps:

[0049] (1) adding butylene glycol and propylene glycol to water of half the amount of the formula and stirring and mixing once at a stirring speed of 150 r / min, and then adding apple extract, Tremella fuciformis fruiting body extract, Glycyrrhiza glabra extract, Psoralea corylifolia extract, 4-methoxysalicylic acid potassium salt, and niacinamide PC and stirring and mixing for a second time at a stirring speed of 350 r / min to obtain a mixed material A;

[0050] (2) adding hydroxyethyl cellulose, glyceryl stearate, and lecithin to the other half of the water in the formula and stirring at a stirring speed of 300 r / min, and then adding hydroxypinacolone retinoic acid ester, 4-butylresorcinol, and a sunscreen agent and stirring at a stirring speed of 300 r / min to obtain a mixture B;

[0051] (3) Adding mixed material A and preservatives to mixed material B and stirring at a stirring speed of 450 r / min, a light and thin sunscreen with fast film formation is obtained.

[0052] Comparative Example 1

[0053] The difference between this comparative example and Example 1 is that the raw material ratio of the fast-film-forming thin sunscreen is adjusted, and the rest is consistent with Example 1.

[0054] The fast-film-forming thin sunscreen of this comparative example comprises the following raw materials in parts by weight: 4 parts of apple extract, 3 parts of Tremella fuciformis fruiting body extract, 15 parts of Glycyrrhiza glabra extract, 19 parts of Psoralea corylifolia extract, 6 parts of 4-methoxysalicylic acid potassium salt, 11 parts of hydroxypinacolone retinoic acid ester, 2 parts of niacinamide PC, 3 parts of 4-butylresorcinol, 3 parts of sunscreen agent, 3 parts of hydroxyethyl cellulose, 6 parts of butylene glycol, 10.6 parts of propylene glycol, 6.3 parts of glyceryl stearate, 8 parts of lecithin, 0.01 parts of preservative, and 128 parts of water.

[0055] Comparative Example 2

[0056] The difference between this comparative example and Example 1 is that the sunscreen does not contain apple essence and Tremella fuciformis fruiting body extract, and the rest is consistent with Example 1.

[0057] The fast-film-forming thin sunscreen of this comparative example comprises the following raw materials in parts by weight: 35 parts of licorice extract, 26 parts of iris extract, 3 parts of 4-methoxysalicylic acid potassium salt, 7 parts of hydroxypinacolone retinoic acid ester, 7 parts of niacinamide PC, 1.5 parts of 4-butylresorcinol, 6 parts of sunscreen, 2.6 parts of hydroxyethyl cellulose, 2.3 parts of butylene glycol, 2.5 parts of propylene glycol, 3 parts of glyceryl stearate, 4 parts of lecithin, 0.01 parts of preservative, and 128 parts of water;

[0058] The method for preparing the fast-film-forming thin sunscreen of this comparative example is characterized by comprising the following steps:

[0059] (1) adding butylene glycol and propylene glycol to half the amount of water in the formula and stirring at a speed of 180 r / min for a first stirring and mixing, and then adding glycyrrhiza glabra extract, iris oleifera extract, 4-methoxysalicylic acid potassium salt, and niacinamide PC and stirring at a speed of 400 r / min for a second stirring and mixing to obtain a mixed material A;

[0060] (2) adding hydroxyethyl cellulose, glyceryl stearate, and lecithin to the other half of the water in the formula and stirring at a stirring speed of 200 r / min, and then adding hydroxypinacolone retinoic acid ester, 4-butylresorcinol, and a sunscreen agent and stirring at a stirring speed of 200 r / min to obtain a mixture B;

[0061] (3) Adding mixed material A and preservatives to mixed material B and stirring at a stirring speed of 400 r / min, a light and thin sunscreen with fast film formation is obtained.

[0062] Comparative Example 3

[0063] The difference between this comparative example and Example 1 is that the raw materials of the sunscreen do not contain Glycyrrhiza glabra extract, 4-methoxysalicylic acid potassium salt and hydroxypinacolone retinoic acid ester, and the rest are consistent with Example 1.

[0064] The fast-film-forming thin sunscreen of this comparative example comprises the following raw materials in parts by weight: 25 parts of apple extract, 13 parts of Tremella fuciformis fruiting body extract, 22 parts of Psoralea corylifolia extract, 7 parts of niacinamide PC, 1.5 parts of 4-butylresorcinol, 6 parts of sunscreen, 2.6 parts of hydroxyethyl cellulose, 5.3 parts of butylene glycol, 9.5 parts of propylene glycol, 3 parts of glyceryl stearate, 4 parts of lecithin, 0.01 parts of preservative, and 128 parts of water;

[0065] The method for preparing the fast-film-forming thin sunscreen of this comparative example is characterized by comprising the following steps:

[0066] (1) adding butylene glycol and propylene glycol to half the amount of water at a stirring speed of 180 r / min, stirring and mixing once, and then adding apple extract, Tremella fuciformis fruiting body extract, Psoralea corylifolia extract, and niacinamide PC, stirring and mixing twice at a stirring speed of 400 r / min to obtain a mixed material A;

[0067] (2) adding hydroxyethyl cellulose, glyceryl stearate, and lecithin to the other half of the water in the formula and stirring at a stirring speed of 200 r / min, and then adding 4-butylresorcinol and a sunscreen agent and stirring at a stirring speed of 200 r / min to obtain a mixture B;

[0068] (3) Adding mixed material A and preservatives to mixed material B and stirring at a stirring speed of 400 r / min, a light and thin sunscreen with fast film formation is obtained.

[0069] Comparative Example 4

[0070] The difference between this comparative example and Example 1 is that the raw materials of the sunscreen do not contain niacinamide PC and 4-butylresorcinol, and the rest are consistent with Example 1.

[0071] The fast-film-forming thin sunscreen of the present embodiment comprises the following raw materials in parts by weight: 25 parts of apple extract, 13 parts of Tremella fuciformis fruiting body extract, 10 parts of Glycyrrhiza glabra extract, 12 parts of Psoralea corylifolia extract, 4.5 parts of 4-methoxysalicylic acid potassium salt, 7 parts of hydroxypinacolone retinoic acid ester, 6 parts of sunscreen, 2.6 parts of hydroxyethyl cellulose, 2.3 parts of butylene glycol, 9.5 parts of propylene glycol, 3 parts of glyceryl stearate, 4 parts of lecithin, 0.01 parts of preservative, and 128 parts of water;

[0072] The method for preparing the fast-film-forming thin sunscreen of this comparative example is characterized by comprising the following steps:

[0073] (1) adding butylene glycol and propylene glycol to water in a half amount of the formula and stirring and mixing once at a stirring speed of 180 r / min, and then adding apple extract, Tremella fuciformis fruiting body extract, Glycyrrhiza glabra extract, Psoralea corylifolia extract, and 4-methoxysalicylic acid potassium salt and stirring and mixing for a second time at a stirring speed of 400 r / min to obtain a mixed material A;

[0074] (2) adding hydroxyethyl cellulose, glyceryl stearate, and lecithin to the other half of the water in the formula and stirring at a stirring speed of 200 r / min, and then adding hydroxypinacolone retinoic acid ester and sunscreen agent and stirring at a stirring speed of 200 r / min to obtain a mixture B;

[0075] (3) Adding mixed material A and preservatives to mixed material B and stirring at a stirring speed of 400 r / min, a light and thin sunscreen with fast film formation is obtained.

[0076] The sunscreens prepared in the above Examples 1-3 and Comparative Examples 1-4 were subjected to performance tests.

[0077] 1. Sunscreen performance test: The SPF value and PA value of the sunscreens prepared in Examples 1-3 and Comparative Examples 1-4 were measured using a UV-2000S sunscreen performance analyzer.

[0078] 2. According to the CALT / TM / SOP287-01 standard operating procedure for in vitro skin model efficacy evaluation test, the effects of the sunscreens prepared in Examples 1-3 and Comparative Examples 1-4 on the epidermal model containing melanocytes were tested to determine the proportion of melanin particles in the epidermal model containing melanocytes.

[0079] 2.1 Experimental Materials

[0080] 2.1.1 Detection kit: 3D printed human skin models;

[0081] 2.1.2 Culture conditions: 37°C, 5% CO2, relative humidity 95%;

[0082] 2.1.3 Culture medium: human in vitro recombinant melanin model culture medium (Lot: 20231211);

[0083] 2.2 Experimental procedures

[0084] 2.2.1 Skin model preparation: Add pre-warmed culture medium into a 6-well culture plate (1 mL well), transfer the epidermal model containing melanocytes into the 6-well plate containing culture medium, and place in an incubator for overnight culture;

[0085] 2.2.2 Sample addition: Take 25 μL of the sunscreen prepared in Examples 1-3 and Comparative Examples 1-4 as test samples, take the same amount of PBS as a control group, and take the same amount of kojic acid as a positive group, evenly apply it on the model surface, and then expose it at room temperature for 1 hour, and set 3 parallels for each test group; after reaching the specified exposure time, rinse the sample thoroughly with DPBS until there is no residue, and then place it in a six-well plate containing fresh maintenance culture medium and continue incubation for 48 hours;

[0086] 2.2.3 After the specified incubation time, carefully remove the model from the well plate with tweezers, then use a punch to punch out the skin tissue, and then use a sharp blade to cut the tissue for value determination and melanin particle distribution measurement experiments.

[0087] 2.2.4 Melanin staining: fix the removed experimental tissue in 4% paraformaldehyde solution, then use 30% sucrose solution to fully dehydrate, and finally use OCT frozen section embedding agent to embed. During the fixation process, be careful to remove the bubbles in the OCT glue, and then quickly place it in liquid ammonia. After marking the groups, transfer it to -80℃ for storage. After tissue sectioning, use the melanin staining kit to stain it. Refer to the instructions of Shangbao Biological Melanin Staining Kit to determine the proportion of melanin particles.

[0088] Table 1 Performance test results of sunscreens prepared in Examples 1-3 and Comparative Examples 1-4

[0089]

[0090] *: Compared with the control group, the difference is statistically significant (p<0.05)

[0091] The above results show that the sunscreen prepared by the method described in Examples 1-3 of the present invention has good sunscreen performance and can effectively reduce the generation and deposition of melanin. Compared with Example 1, Comparative Example 1 adjusts the raw material ratio of the sunscreen, Comparative Example 2 does not contain apple essence and Tremella fruiting body extract, Comparative Example 3 does not contain Glycyrrhiza glabra extract, 4-methoxysalicylic acid potassium salt and hydroxypinacolone retinoic acid ester, and the raw materials of the sunscreen of Comparative Example 4 do not contain nicotinamide PC and 4-butylresorcinol. Due to unreasonable raw material matching, Comparative Examples 1-4 cause the sunscreen performance of the sunscreen to decrease and the pigment is easy to deposit.

[0092] The sunscreen prepared in Example 1 of the present invention was evaluated for its efficacy. The test start and end time was October 28, 2023 to November 25, 2023. 33 subjects were recruited, aged 18 to 55 years old, including 4 males and 29 females, with an average age of 45.30±5.88 years old. The inclusion criteria were: skin type was neutral, mixed dry or dry skin, sensitive skin, accompanied by sensitive symptoms such as redness and red blood streaks. The night before the test, the subjects washed their faces without applying any skin care products on their faces. After basic skin care, an appropriate amount of the product of the present invention was evenly applied to the face, neck and skin easily exposed to the sun, once a day, for 28 consecutive days. Then, relevant performance parameters (skin stratum corneum moisture content, transepidermal water loss value, skin melanin percentage, skin spot percentage) were tested before use (D0), 14 days after use (D14), and 28 days after use (D28). After 14 days (D14) and 28 days (D28), the subjects were asked to fill in a self-assessment questionnaire on the skin improvement status after using the sunscreen sample of the present invention, and the use evaluation score of each subject was calculated.

[0093] The standard operating procedures are as follows:

[0094] (1) GHS-SOP-G-101 Skin Moisture Content Test Method;

[0095] (2) GHS-SOP-G-102 transepidermal water loss test method;

[0096] (3) GHS-SOP-G-105 facial image acquisition and analysis methods;

[0097] (4) GHS-SOP-G-121 Skin Melanin Test Method

[0098] The performance parameter test instruments are as follows:

[0099] 1) Skin stratum corneum moisture content test

[0100] Use probe CM825 measures the moisture content of the skin stratum corneum at each test time point.

[0101] 2) Epidermal water loss test

[0102] Use probe TMHex measures the transepidermal water loss (TEWL) [g / (h·m 2 )].

[0103] 3) Skin melanin test

[0104] Use probe MX18 measures skin melanin at each test time point.

[0105] 4) Spot area ratio test

[0106] The skin image acquisition instrument VISIA-CR was used to capture the subjects' images, and the analysis system Image-Pro Plus was used to analyze the percentage of pigmentation area.

[0107] The result is shown below.

[0108] Table 2 Skin stratum corneum moisture content test results

[0109]

[0110] *p<0.05, there is a significant difference after use compared with before use.

[0111] Table 3 Transepidermal water loss values ​​[g / (h·m 2 )]Test results

[0112]

[0113] *p<0.05, there is a significant difference after use compared with before use.

[0114] Table 4 Skin melanin detection results

[0115]

[0116]

[0117] *p<0.05, there is a significant difference after use compared with before use.

[0118] Table 5. Results of color spot area ratio detection

[0119]

[0120] *p<0.05, there is a significant difference after use compared with before use.

[0121] From Table 2-5 and Figure 1-4 It can be seen that the sunscreen of the present invention can keep the skin moisturized after being used for 14 to 28 days compared with before use, can effectively resist ultraviolet radiation, can reduce the production of melanin after sun exposure, reduce skin spots and dullness, improve the sunscreen and whitening effects, and has a good effect.

[0122] Table 6 Skin feel rating results of the subjects using the products

[0123]

[0124] Scoring criteria: 1 point means strongly disagree, 2 points means somewhat disagree, 3 points means basically disagree, 4 points means basically agree, 5 points means somewhat agree, and 6 points means strongly agree.

[0125] Recognition degree = number of subjects with a score of 24 / valid number of subjects × 100%

[0126] The results in Table 6 above show that the sunscreen prepared by the present invention has a good use effect. It can be easily pushed open and easily form a sunscreen protective film during use. It is thin and non-sticky and effectively resists ultraviolet rays. It feels light and burden-free on the skin. It can also improve the moisture of the skin, prevent the skin from sunburn and redness, and also prevent the skin from tanning and aging.

[0127] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A fast-film-forming, light sunscreen, characterized in that: The invention comprises the following raw materials in parts by weight: 19-25 parts of apple essence, 13-17 parts of Tremella fuciformis fruiting body extract, 7-10 parts of Glycyrrhiza glabra extract, 9-14 parts of Psoralea corylifolia extract, 1-4 parts of 4-methoxysalicylic acid potassium salt, 4-7 parts of hydroxypinacolone retinoic acid ester, 5-8 parts of niacinamide PC, 1-3 parts of 4-butylresorcinol, 4-8 parts of sunscreen, 2-4 parts of hydroxyethyl cellulose, 2-4 parts of butylene glycol, 1-3 parts of propylene glycol, 2-4 parts of glyceryl stearate, 2-4 parts of lecithin, 0.01-0.05 parts of preservative and 120-150 parts of water.

2. The fast-film-forming, light sunscreen according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 25 parts of apple extract, 13 parts of Tremella fuciformis fruiting body extract, 10 parts of Glycyrrhiza glabra extract, 12 parts of Psoralea corylifolia extract, 3 parts of 4-methoxysalicylic acid potassium salt, 7 parts of hydroxypinacolone retinoic acid ester, 7 parts of niacinamide PC, 1.5 parts of 4-butylresorcinol, 6 parts of sunscreen, 2.6 parts of hydroxyethyl cellulose, 2.3 parts of butylene glycol, 2.5 parts of propylene glycol, 3 parts of glyceryl stearate, 4 parts of lecithin, 0.01 parts of preservative and 128 parts of water.

3. The fast-film-forming, light sunscreen according to claim 1, characterized in that: The sunscreen is one or both of titanium dioxide and zinc oxide.

4. The fast-film-forming, light sunscreen according to claim 1, characterized in that: The preservative is one or both of methylparaben and propylparaben.

5. The fast-film-forming, light sunscreen according to claim 1, characterized in that: The preparation method of the apple essence comprises the following steps: washing fresh apples, crushing them and squeezing out the juice; freezing and drying the apple pomace and then crushing them to obtain apple pomace powder; adding the apple pomace powder to an aqueous solution containing 2-5% of Vc by mass and stirring and mixing them evenly; adding a composite enzyme to perform enzymolysis at 40-50° C. for 50-60 minutes; maintaining the pressure at 300-500 MPa for 5-7 minutes; filtering; and decompressing and concentrating the filtrate to obtain the apple essence.

6. The fast-film-forming, light sunscreen according to claim 5, characterized in that: The ratio of apple pomace powder to aqueous solution is 1:15-18 g / mL; the mass of the added complex enzyme is 1-1.4% of the aqueous solution; the complex enzyme comprises beta-glucanase and pectinase in a mass ratio of 3-5:1-2.

7. The method for preparing the fast-film-forming, light and thin sunscreen according to any one of claims 1 to 6, characterized in that: The following steps are involved: (1) adding butylene glycol and propylene glycol to half the amount of water and stirring once, then adding apple extract, Tremella fuciformis fruiting body extract, Glycyrrhiza glabra extract, Psoralea corylifolia extract, 4-methoxysalicylic acid potassium salt, and niacinamide PC and stirring twice to obtain a mixed material A; (2) adding hydroxyethyl cellulose, glyceryl stearate, and lecithin to the other half of the water in the formula and stirring to mix, and then adding hydroxypinacolone retinoic acid ester, 4-butylresorcinol, and a sunscreen agent and stirring to mix to obtain a mixture B; (3) Adding mixed material A and preservatives to mixed material B and stirring to obtain the target sunscreen.

8. The method for preparing the fast-film-forming, light and thin sunscreen according to claim 7, characterized in that: In step (1), the first stirring speed is 150-180 r / min, and the second stirring speed is 300-400 r / min.

9. The method for preparing the fast-film-forming, light and thin sunscreen according to claim 7, characterized in that: In step (2), the stirring speed is 200 to 300 r / min; in step (3), the stirring speed is 350 to 450 r / min.

10. Use of the fast-film-forming, light-weight sunscreen according to any one of claims 1 to 6 in the preparation of skin care products.

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