Traditional Chinese medicine for adjuvant therapy of AIDS and preparation method thereof

Abstract

The invention discloses application of a traditional Chinese medicine composition in preparation of traditional Chinese medicines for adjuvant therapy of AIDS. The traditional Chinese medicine composition is mainly prepared from the following medicinal materials: radix bupleuri, celosia cristata, salvia miltiorrhiza, fructus lycii, liquorice, artemisia capillaris, snake slough, schisandra chinensis, cordyceps sinensis and pig bile powder. The traditional Chinese medicine for adjuvant therapy of AIDS has the effects of resisting HIV, soothing liver-qi stagnation and tonifying spleen and qi, and can be used for clearing away heat and toxic materials, resisting inflammation and viruses and adjuvant therapy of AIDS. The traditional Chinese medicine composition has the pharmacological effects of resisting viruses, resisting inflammation and the like, can effectively inhibit reverse transcription of viruses, and has a good treatment effect on HIV (human immunodeficiency virus) and HBV (hepatitis B virus) infection. In addition, main components of the traditional Chinese medicine composition come from the nature, so that the traditional Chinese medicine composition has the advantages of being natural and safe, and has good safety in clinical application.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a traditional Chinese medicine for auxiliary treatment of AIDS and a preparation method thereof. Background Art

[0002] AIDS, the full name of which is acquired immunodeficiency syndrome, is an infectious disease with a high mortality rate and no cure, which seriously threatens human health. HIV belongs to the genus Lentivirus of the family Retroviridae and is a spherical virus with a diameter of about 100 nm. Since HIV is an RNA virus, its replicase has no proofreading function and is therefore highly mutagenic. At present, the commonly used anti-HIV drugs in China include more than 30 types in six categories, including nucleoside reverse transcriptase inhibitors, non-nucleoside reverse transcriptase inhibitors, and protease inhibitors. Although highly effective antiretroviral therapy can effectively control HIV replication and restore or partially rebuild the patient's immune function, it cannot completely eliminate HIV in the human body, and patients need to take medication for life. Since these drugs have large toxic side effects and adverse reactions, and HIV's drug resistance is also gradually increasing, it is urgent to seek new and effective anti-HIV drugs.

[0003] Due to its unique advantages, Chinese medicine has been developed and spread in my country for thousands of years and has accumulated rich clinical experience. Chinese medicine has multiple pharmacological effects such as antiviral and immune enhancement, and has relatively few side effects. In addition, Chinese medicine compound compatibility also has the advantages of synergistic enhancement and reduced toxic side effects.

[0004] The applicant previously applied for a Chinese patent for "a Chinese medicine for treating viral hepatitis and its preparation method" (patent number 201010223375.6, published on November 3, 2010). The medicine has a good inhibitory effect on HBV and provides a new medicine for treating viral hepatitis that is safe, easy to take, and has no toxic side effects (referred to as the Pinggan Jiedu prescription). The applicant added or subtracted the dosage of individual Chinese medicines in the original formula and applied it to the auxiliary treatment of HIV-infected patients, achieving satisfactory results. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a traditional Chinese medicine for auxiliary treatment of AIDS and a preparation method thereof.

[0006] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0007] The invention discloses an application of a Chinese medicine composition in preparing a Chinese medicine for assisting in the treatment of AIDS. The Chinese medicine composition is mainly made of the following medicinal materials: bupleurum, cockscomb, salvia miltiorrhiza, wolfberry, liquorice, artemisia selengensis, snake skin, schisandra chinensis, cordyceps sinensis, and pig bile powder.

[0008] The Chinese medicine composition is mainly made of the following medicinal materials by weight: 18-22 parts of bupleurum, 37-42 parts of cockscomb, 28-32 parts of salvia miltiorrhiza, 13-17 parts of wolfberry, 13-17 parts of liquorice, 28-32 parts of artemisia selengensis, 3-7 parts of snake skin, 18-22 parts of schisandra, 8-12 parts of cordyceps and 8-12 parts of pig bile powder.

[0009] The Chinese medicine composition is mainly made of the following medicinal materials by weight: 20 parts of bupleurum, 50 parts of cockscomb, 30 parts of salvia miltiorrhiza, 15 parts of wolfberry, 15 parts of liquorice, 30 parts of artemisia selengensis, 5 parts of snake skin, 20 parts of schisandra, 10 parts of cordyceps, and 10 parts of pig bile powder.

[0010] Traditional Chinese medicine for auxiliary treatment of AIDS is an oral preparation.

[0011] The preparation method of the above-mentioned Chinese medicine for auxiliary treatment of AIDS is carried out according to the following steps:

[0012] <1> Grind the dried Schisandra chinensis, wolfberry, snake skin and Cordyceps sinensis into fine powder respectively, and then sieve to obtain fine and uniform powder;

[0013] <2> Step <1> The obtained four kinds of powders are mixed evenly and set aside;

[0014] <3> Add bupleurum, liquorice, salvia miltiorrhiza, artemisia selengensis and cockscomb into clear water and soak; decoct after soaking, concentrate the decoction into extract, and dry to make dry extract;

[0015] <4> Step <3> The obtained dry extract is ground into fine powder and set aside;

[0016] <5> Step <4> Obtained dry extract powder and steps <2> The obtained mixed powder and pig bile powder are mixed and stirred evenly, dried and crushed into fine powder, and sieved to obtain the Chinese medicine composition powder.

[0017] step <1> The drying temperature is 60°C and the sieving is performed using a 100-mesh sieve.

[0018] step <3> Soak for 30 minutes; decoct twice, the first time with the soaking liquid for 2 hours, the second time with water decoction for 2 hours, the amount of water for the two decoctions is 8 times the amount of medicinal materials, and the two decoctions are combined; the relative density of the extract is 1.36 at 80℃.

[0019] step <5> In the process, a 100-mesh sieve is used for sieving.

[0020] The Chinese medicine for auxiliary treatment of AIDS is capsule, and the steps are <5> The obtained Chinese medicine composition powder is filled into No. 1 capsules, and each capsule weighs 0.3 g.

[0021] The traditional Chinese medicine for auxiliary treatment of AIDS obtained by the above preparation method.

[0022] Based on the previous research on the "Pinggan Jiedu Prescription", the inventor further discovered that the Chinese medicine composition also has an inhibitory effect on HIV and has potential application value in the preparation of Chinese medicine for the auxiliary treatment of AIDS. The present invention has advantages in alleviating the symptoms of AIDS patients, enhancing immune function and improving liver function. Since the various components in the Chinese medicine composition can produce a synergistic effect to enhance antiviral activity, this synergistic effect can reduce the risk of drug resistance and improve the therapeutic effect; Chinese medicine compositions usually have good safety and less side effects, which makes them safer and more reliable when used for a long time; Chinese medicine compositions are usually more cost-effective than Western medicines, which makes them more accessible in resource-limited areas or countries; Chinese medicine compositions have a history of thousands of years of application in traditional medicine and have accumulated rich experience, which makes them have advantages in research and development and clinical application. Chinese medicine compositions are usually composed of natural ingredients and are sustainable. This makes them have advantages in environmental protection.

[0023] In summary, the Chinese medicine for the auxiliary treatment of AIDS of the present invention (i.e., the Pinggan Jiedu prescription) has the effects of anti-HIV, soothing the liver and relieving depression, and tonifying the spleen and replenishing qi, and can be used for clearing away heat and detoxification, anti-inflammatory and antiviral, and auxiliary treatment of AIDS. Among them, the Chinese medicine composition has pharmacological effects such as antiviral and anti-inflammatory, can effectively inhibit the reverse transcription of viruses, and has a good therapeutic effect on HIV and HBV infection. In addition, the main components of the Chinese medicine composition of the present invention come from nature, have the advantages of being natural and safe, and have good safety in clinical applications. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The present invention is a process flow chart of the preparation method of the Chinese medicine for auxiliary treatment of AIDS.

[0025] Figure 2 This is a graph showing the inhibitory effect of the blood-transmitted components of the "Pinggan Jiedu Prescription" of the present invention on HIV pseudovirus-infected TZM-b1 cells.

[0026] Figure 3 This is a diagram of the network pharmacology analysis results of the Chinese herbal composition for auxiliary treatment of AIDS of the present invention, in which: Figure A, Figure B, Figure C, Figure D and Figure E are GO and KEGG enrichment analysis of key targets of AIDS regulated by "Pingan Jiedu Prescription"; Figure F is a component-target-pathway network diagram of "Pingan Jiedu Prescription" for treating AIDS (Note: red represents genes, green represents pathways, and blue represents chemical components); Figure G is a molecular docking hotspot map of main core components and key targets. DETAILED DESCRIPTION

[0027] 1. Formula design

[0028] The liver-calming and detoxifying prescription of the present invention is mainly made of the following medicinal materials: bupleurum, cockscomb, salvia miltiorrhiza, wolfberry, liquorice, artemisia selengensis, snake skin, schisandra, cordyceps, and pig bile powder. The standards of the selected Chinese herbal medicine raw materials are:

[0029] Bupleurum: Volume 1 of the 2020 edition of the Pharmacopoeia of the People's Republic of China.

[0030] Celosia: One volume of the 2020 edition of the Pharmacopoeia of the People's Republic of China.

[0031] Salvia miltiorrhiza: Volume 1 of the 2020 edition of the Pharmacopoeia of the People's Republic of China.

[0032] Lycium barbarum: "Pharmacopoeia of the People's Republic of China" 2020 edition.

[0033] Licorice: Pharmacopoeia of the People's Republic of China, 2020 edition.

[0034] Artemisia capillaris: Volume 1 of the 2020 edition of the Pharmacopoeia of the People's Republic of China.

[0035] Snake skin: "Pharmacopoeia of the People's Republic of China" 2020 edition.

[0036] Schisandra chinensis: Pharmacopoeia of the People's Republic of China 2020 edition.

[0037] Cordyceps sinensis: "Pharmacopoeia of the People's Republic of China" 2020 edition.

[0038] Pig bile powder: "Pharmacopoeia of the People's Republic of China" 2020 edition.

[0039] Mechanism of action: Some ingredients in the Chinese medicine composition of the present invention can resist HIV, such as various effective ingredients extracted from Artemisia capillaris, Salvia miltiorrhiza, and Schisandra chinensis have been found to have anti-HIV effects. Bupleurum and Licorice have the effects of soothing the liver and relieving depression, and tonifying the spleen and replenishing qi. Celosia cristata can supplement a variety of nutrients for the human body and promote body metabolism. Lycium barbarum and Cordyceps sinensis can nourish the kidney and liver, and relieve the symptoms of asthenia and deficiency of essence. Snake slough and pig bile powder can clear away heat and detoxify and resist various inflammations. This Chinese medicine prescription has a potential therapeutic effect on AIDS from the perspectives of antiviral and body conditioning. In addition, some ingredients in the Chinese medicine composition of the present invention have anti-inflammatory effects, which can reduce the inflammatory response caused by viral infection, such as bile acid in pig bile powder can inhibit the release of inflammatory factors, thereby reducing the inflammatory response. Finally, the various ingredients in the Chinese medicine composition of the present invention can act synergistically with each other to enhance the antiviral efficacy. For example, schisandrin in Schisandra chinensis can enhance the antiviral activity of isatis saponins in Radix Isatidis, and bile acid in pig bile powder can enhance the antiviral activity of saikosaponins in Radix Bupleuri.

[0040] 2. Preparation process

[0041] Formula and proportion (g): 20 parts of bupleurum, 40 parts of cockscomb, 30 parts of salvia miltiorrhiza, 15 parts of wolfberry, 15 parts of licorice, 30 parts of artemisia selengensis, 5 parts of snake skin, 20 parts of schisandra, 10 parts of cordyceps, and 10 parts of pig bile powder.

[0042] Preparation method (such as Figure 1 shown):

[0043] <1> Wash and sterilize Schisandra chinensis, wolfberry, snake skin and Cordyceps sinensis, dry them at 60℃, grind them into fine powder in a grinder or pulverizer, and then pass them through a 100-mesh sieve to obtain fine and uniform powder;

[0044] <2> Step <1> The four powders are mixed evenly and set aside. When mixing, clean containers and tools should be used to avoid impurities. The mixed powders should be placed in a cool and dry place, away from direct sunlight and high temperature.

[0045] <3> Soak bupleurum, licorice, salvia miltiorrhiza, artemisia selengensis and cockscomb in water (30 minutes, the amount of water added is 4 times the amount of medicinal materials); decoct after soaking, decoct twice, the first time directly decoct with the soaking liquid for 2 hours, the second time add 4 times the amount of medicinal materials water and decoct for another 2 hours, combine the two decoctions; concentrate the decoction into an extract, the relative density of the extract is 1.36 at 80℃. Dry to make a dry extract;

[0046] <4> Step <3> The obtained dry extract is placed in a grinder or a pulverizer and ground into fine powder for later use;

[0047] <5> Step <4> Obtained dry extract powder and steps <2> The obtained mixed powder and pig bile powder are mixed and stirred evenly, dried and crushed into fine powder, and passed through a 100-mesh sieve to obtain a Chinese medicine composition powder.

[0048] <6> Step <5> The obtained Chinese medicine composition powder is filled into No. 1 capsules, and each capsule weighs 0.3 g.

[0049] Dosage and Usage of Capsules: Take 15-25 minutes before meals, 6 capsules at a time, three times a day.

[0050] 3. Clinical Application

[0051] 3.1 Preclinical pharmacology experiments

[0052] 3.1.1 Experimental Example 1 Anti-HIV in vitro experiment

[0053] Prepare cell culture medium containing extracts of series concentrations (i.e., the powder of the Chinese medicine composition of "Pinggan Jiedu Prescription" obtained by the above preparation process 2), and determine the cytotoxicity of the drug by MTT method. Take the highest non-toxic concentration as the benchmark, test the inhibitory effect of the extract on HIV virus-infected cells with HIV pseudovirus, and calculate the virus half-inhibitory dose (IC50) and selectivity index (SI).

[0054] The extract of the prescription was gavaged into mice at 40 times the human dosage, and the mouse serum was obtained and filtered for sterilization. HIV pseudovirus was used to test the inhibitory effect of the drug-containing serum on HIV virus-infected cells, and the IC50 and SI were calculated.

[0055] 3.1.2 Results

[0056] like Figure 2 As shown, the IC50 concentration of the blood-entering components of the "Pingan Jiedu Prescription" for inhibiting HIV pseudovirus infection is about 35.6% lower than that of the marketed Chinese patent medicine "Tangcao Tablets", indicating that it has a higher antiviral activity.

[0057] 3.2 Clinical trials

[0058] 3.2.1 Select Object

[0059] A total of 107 AIDS patients with initial treatment and good compliance who were admitted to the AIDS clinic of Beihai People's Hospital from January 2016 to June 2018 were selected, including 76 males and 31 females with an average age of (39.5±11.6) years.

[0060] 3.2.2 Research Methods

[0061] Patients were divided into two groups according to whether they received Chinese medicine treatment: 42 cases in the HAART combined with "Pinggan Jiedu Prescription" group and 65 cases in the simple HAART group. At 48 weeks of treatment, the changes in immune function and virological response rate of the two groups of patients were compared. Effective treatment means that after HAART for 1 year, ① virological indicators: HIV RNA is below the detection limit to achieve virological suppression. ② Immunological indicators: Compared with before treatment, the CD4+T lymphocyte count increased by 30% or increased by 100 / μL.

[0062] HAART regimen: TDF+3TC+EFV or LPV / r

[0063] The IL-28Brs12979860 gene of the patients was typed by direct sequencing of PCR products. The peripheral blood CD4+T lymphocyte counts of the patients were detected by flow cytometry before treatment and at 48 weeks of treatment.

[0064] 3.2.3 Results

[0065] After 48 weeks of antiviral treatment, univariate regression analysis was performed on the influencing factors of the efficacy of 107 AIDS patients. The results showed that age at the initial treatment, IL-28B genotype, CD4+T lymphocyte count, whether the antiviral treatment regimen contained protease inhibitors, combined treatment with "Pinggan Jiedu Prescriptions", and the presence of more than one opportunistic infection had an impact on the antiviral efficacy, as shown in Table 1.

[0066] Comparison of adverse reactions between patients who received and did not receive "Pingan Jiedu Prescription" showed that the incidence of abnormal liver function in the HAART combined with "Pingan Jiedu Prescription" group was 11.90% (5 / 42), which was lower than 33.85% (22 / 65) in the HAART alone group, and the difference was statistically significant (χ2=4.070, P<0.05).

[0067] Table 1 Univariate regression analysis of factors affecting the efficacy of antiviral therapy in AIDS patients

[0068]

[0069]

[0070] 4. Network Pharmacology

[0071] 4.1 Research Methods

[0072] The chemical composition of Danshen, Celosia cristata, Schisandra chinensis, Licorice, Lycium barbarum and Cordyceps sinensis was queried through the TCMSP platform. After ADME screening, 272 effective ingredients of "Pinggan Jiedu Prescription" were obtained. After screening the targets corresponding to each Chinese medicine ingredient, 344 effective targets of "Pinggan Jiedu Prescription" were obtained after duplication. After screening the relevant targets of AIDS obtained from each database, 1,357 relevant targets of AIDS were obtained after duplication.

[0073] The potential targets of AIDS and "Pinggan Jiedu Prescription" were intersected, and 156 common targets were obtained. According to the GO and KEGG enrichment analysis of the key targets of "Pinggan Jiedu Prescription" regulating AIDS, the main biological functions of the key targets and the main pathways involved were found, so as to speculate the molecular mechanism of "Pinggan Jiedu Prescription" regulating AIDS. The results are shown in Figure 3 -A. Figure 3 -B. Figure 3 -C. Figure 3 -D. Figure 3 -E. KEGG analysis results showed that the genes of "Pinggan Jiedu Prescription" for the treatment of AIDS were mainly enriched in pathways such as PI3K-Akt signaling pathway and TNF signaling pathway.

[0074] The potential targets of "Pingan Jiedu Prescription" for regulating AIDS were imported into the STRING database to obtain the protein PPI network, and 22 key targets were obtained. The PPI network was further analyzed to obtain 5 sub-networks. GO biological process analysis was performed on each MCODE, and according to the p-value, the 3 best scores were retained and their functions were described.

[0075] There are 243 active ingredients corresponding to the key targets of "Pingan Jiedu Prescription" for regulating AIDS. The top 20 pathways of KEGG were selected and the ingredient-target-pathway network diagram of "Pingan Jiedu Prescription" for treating AIDS was constructed by CytoScape3.8.0. Figure 3 -F.

[0076] According to the degree calculated by CytoScape, the key components and targets were screened, and 38 main active ingredients were obtained, including quercetin, luteolin, kaempferol, naringenin, tanshinone IIA, etc. (see Table 2). From the component-target-pathway network diagram, it can be seen that one component can be connected to multiple targets, and one target may also be related to multiple components, and the targets and pathways are also connected in multiple lines, suggesting that the mechanism of "Pinggan Jiedu Prescription" in treating AIDS may be characterized by multiple components, multiple targets, and multiple pathways.

[0077] The top 6 protein targets ranked by PPI network degree were molecularly docked with the top 10 main active ingredients ranked in Table 2. The docking results are shown in Figure 3 -G. The docking results showed that the binding energies of these 10 main components with the protein targets were all less than -5 kcal·mol-1, indicating good binding activity.

[0078] 4.2 Results

[0079] The "Pinggan Jiedu Prescription" contains chemical components with potential effects in treating AIDS, including quercetin, luteolin, kaempferol, naringenin, tanshinone IIA, etc. Quercetin has a good anti-HIV-1 effect. The three types of quercetin and kaempferol isolated from Euphorbia by Ahn et al. can inhibit the activity of HIV-1 integrase. Luteolin can downregulate the expression of CDK9 and HIV-Tat proteins, and inactivate the virus hidden in CD4+T cells with spontaneous induction and replication capabilities by continuously enhancing the HIV latent period.

[0080] The "Pinggan Jiedu Prescription" treats AIDS through a multi-component, multi-target, and multi-pathway network mechanism. Corresponding to the molecular docking and clinical results, it shows that the "Pinggan Jiedu Prescription" can be used in combination with Western medicine to have an auxiliary therapeutic effect on AIDS.

[0081] Table 2

[0082]

[0083]

[0084] Existing drugs for treating AIDS can improve the prognosis of most patients, but the drugs have great toxic side effects, especially great toxicity to the liver. The Chinese medicine composition for auxiliary treatment of AIDS in the present invention can improve liver function on the basis of assisting antiviral treatment, and the Chinese medicine composition can simultaneously inhibit HBV and HIV infection, thereby reducing the possibility of viral resistance. In addition, as a composite Chinese medicine, the Chinese medicine composition of the present invention uses natural plant and animal ingredients as raw materials, which are considered to be relatively safe. Compared with synthetic antiviral drugs, they usually have lower side effects and toxicity. The Chinese medicine composition of the present invention usually contains multiple ingredients, which can act on different targets in the viral life cycle, thereby inhibiting the replication of the virus, which makes it more difficult for the virus to develop drug resistance.

[0085] In summary, the Chinese medicine composition for assisting the treatment of AIDS of the present invention is developed on the basis of TCM theory, focusing on the overall concept and syndrome differentiation and treatment, which enables them to regulate the human immune system and enhance the body's antiviral ability while treating viral infections, and has potential application prospects in the auxiliary treatment of AIDS in combination with Western medicine.

Claims

1. Use of a Chinese medicine composition in the preparation of a Chinese medicine for the auxiliary treatment of AIDS, characterized in that: The Chinese medicine composition is mainly prepared from the following medicinal materials: bupleurum, cockscomb, salvia miltiorrhiza, wolfberry, liquorice, artemisia selengensis, snake skin, schisandra chinensis, cordyceps sinensis and pig bile powder.

2. The use according to claim 1, characterized in that: The Chinese medicine composition is mainly prepared from the following medicinal materials by weight: 18-22 parts of bupleurum, 38-42 parts of cockscomb, 28-32 parts of salvia miltiorrhiza, 13-17 parts of wolfberry, 13-17 parts of liquorice, 28-32 parts of artemisia selengensis, 3-7 parts of snake skin, 18-22 parts of schisandra, 8-12 parts of cordyceps, and 8-12 parts of pig bile powder.

3. The use according to claim 2, characterized in that: The Chinese medicine composition is mainly prepared from the following medicinal materials by weight: 20 parts of bupleurum, 40 parts of cockscomb, 30 parts of salvia miltiorrhiza, 15 parts of wolfberry, 15 parts of liquorice, 30 parts of artemisia selengensis, 5 parts of snake skin, 20 parts of schisandra, 10 parts of cordyceps, and 10 parts of pig bile powder.

4. The use according to claim 1, characterized in that: The Chinese medicine for auxiliary treatment of AIDS is an oral preparation.

5. The method for preparing the Chinese medicine for assisting in treating AIDS according to claim 4, characterized in that Follow these steps: <1> Grind the dried Schisandra chinensis, wolfberry, snake skin and Cordyceps sinensis into fine powder respectively, and then sieve to obtain fine and uniform powder; <2> Step <1> The obtained four kinds of powders are mixed evenly and set aside; <3> Add bupleurum, liquorice, salvia miltiorrhiza, artemisia selengensis and cockscomb into clear water and soak; decoct after soaking, concentrate the decoction into extract, and dry to make dry extract; <4> Step <3> The obtained dry extract is ground into fine powder and set aside; <5> Step <4> Obtained dry extract powder and steps <2> The obtained mixed powder and pig bile powder are mixed and stirred evenly, dried and crushed into fine powder, and sieved to obtain the Chinese medicine composition powder.

6. The method for preparing a Chinese medicine for assisting in the treatment of AIDS according to claim 5, characterized in that step <1> The drying temperature is 60°C and the sieving is performed using a 100-mesh sieve.

7. The method for preparing a Chinese medicine for assisting in the treatment of AIDS according to claim 5, characterized in that step <3> and soaked for 30 minutes; the decoction was performed twice, the first time the extract was decocted together with the soaking liquid for 2 hours, and the second time water was added and decocted for 2 hours, the amount of water for the two decoctions was 8 times the amount of the medicinal materials, and the two decoctions were combined; the relative density of the extract was 1.36 at 80°C.

8. The method for preparing a Chinese medicine for assisting in treating AIDS according to claim 5, characterized in that step <5> In the process, a 100-mesh sieve is used for sieving.

9. The method for preparing a Chinese medicine for assisting in treating AIDS according to claim 5, characterized in that: The Chinese medicine for auxiliary treatment of AIDS is a capsule, and the steps <5> The obtained Chinese medicine composition powder is filled into No. 1 capsules, and each capsule weighs 0.3 g.

10. A Chinese medicine for assisting the treatment of AIDS obtained by the preparation method according to any one of claims 5 to 9.

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