Extraction process and application of peony flowers

Abstract

The invention provides an extraction process and application of peony flowers. The extraction process of the peony flowers comprises the following steps: step 1, picking fresh peony flowers, performing freeze drying, performing grinding, and performing sieving with a 18-20-mesh sieve so as to obtain freeze-dried powder fragments; step 2, extracting by adopting a Soxhlet extraction device, putting the freeze-dried powder fragments obtained in the step 1 into an extraction pool, adding dichloromethane and diethyl ether as solvents, and performing heating reflux extraction at 55-60 DEG C; and step 3, after the extraction is finished, collecting an extracting solution in the reboiling kettle, and recovering dichloromethane and diethyl ether to obtain the peony flower extracting paste. In the second step, a staged extraction mode is adopted, and the volume ratio of dichloromethane to diethyl ether is gradually changed. According to the invention, extraction can be carried out at a relatively low temperature, the loss of low-boiling-point components caused by high temperature is reduced, and components with different polarities can be well extracted. After the peony flower extract paste is prepared into the perfume, the fragrance can be smelled at normal temperature, and the fragrance can fully restore the flower fragrance of the peony flowers and embody the whole fragrance of the peony flowers.

Description

Technical Field

[0001] The present invention relates to the field of peony extraction, and particularly to an extraction process and application of peonies. Background Art

[0002] Peonies are elegant, magnificent, and known as the king of flowers. They have large flower shapes and beautiful postures, representing grandeur. Besides their ornamental value, peonies can be extracted to obtain an extraction paste for preparing peony essential oil. Peony essential oil has various regulatory functions for the human body, helping to improve mood, resist allergic reactions, improve blood circulation, beautify the skin, promote cell regeneration, relieve skin aging, regulate the skin, balance the hormonal secretion in women's bodies, etc., and can be used as raw materials for medicine, food, beverages, and cosmetics. Further processing peony essential oil into perfume can preserve the fragrance of peonies for a long time.

[0003] Peony essential oil is a volatile oily liquid composed of various volatile components and is obtained from the extraction paste extracted from peonies. The extraction and separation techniques of plant essential oils mainly include steam distillation, solvent extraction, microwave extraction, ultrasonic extraction, supercritical fluid extraction, etc. The supercritical method has high extraction efficiency and high essential oil purity, and maximally preserves the active ingredients of the essential oil. However, the supercritical method mostly uses dried flowers as raw materials to prepare essential oils. During the drying process, a large amount of volatile components in the flowers will be lost. Therefore, the essential oil yield is lower than that prepared from fresh flowers, and the supercritical method requires special equipment with high investment. Steam distillation has simple equipment, easy operation, and low cost, but long-term high-temperature operation is likely to cause decomposition and loss of the effective components of the essential oil. A large number of studies have also been conducted on the most commonly used solvent extraction method. For example, in recent years, many studies have focused on using petroleum ether, propane, butane, and n-hexane as extraction agents. The main components of peony essential oil are alcohols, alkanes, esters, ketones, aromatic hydrocarbons and their derivatives, and also contain a small amount of aldehydes, pyrans, alkenes, and phenols, etc. Among them, phenylethyl alcohol and linalool oxides have relatively large polarities, and these extraction agents are mostly non-polar solvents, with limited extraction efficiency for substances with relatively large polarities.

[0004] In addition, the peony essential oil obtained by high-temperature distillation has a relatively narrow boiling point coverage range of components. After being prepared into perfume, it cannot fully reflect the full fragrance of peonies. Summary of the Invention

[0005] In view of this, the present invention aims to propose an extraction process and application of peonies to solve the problem that low-boiling components are easily lost during the extraction of peonies, resulting in incomplete extraction and affecting the yield and quality of the extract.

[0006] To achieve the above object, the technical solution of the present invention is realized as follows:

[0007] On the one hand, the present invention provides an extraction process for peonies, comprising the following steps:

[0008] Step 1: Pick fresh peonies, freeze-dry them, and then grind them through an 18-20 mesh sieve to obtain a freeze-dried powder.

[0009] Step 2: Use a Soxhlet extraction apparatus for extraction. Place the freeze-dried powder obtained in Step 1 into the extraction pool, add dichloromethane and ether as solvents, and perform heating reflux extraction at 55-60 °C.

[0010] Step 3: After the extraction is completed, collect the extract in the reboiler, recover dichloromethane and ether, and obtain a peony extract paste.

[0011] Furthermore, when adding dichloromethane and ether as solvents for heating reflux extraction in Step 2, a staged extraction method is adopted, specifically as follows:

[0012] First stage: Mix dichloromethane and ether in a volume ratio of 6:1 as the solvent and place it in the reboiler. Perform heating reflux extraction at 55-60 °C for 1-1.5 h, and collect the extract in the reboiler.

[0013] Second stage: Mix dichloromethane and ether in a volume ratio of 3:1 as the solvent and place it in the reboiler. Perform heating reflux extraction at 55-60 °C for 1-1.5 h, and collect the extract in the reboiler.

[0014] Third stage: Mix dichloromethane and ether in a volume ratio of 1:1 as the solvent and place it in the reboiler. Perform heating reflux extraction at 55-60 °C for 1-1.5 h, and collect the extract in the reboiler.

[0015] Fourth stage: Mix dichloromethane and ether in a volume ratio of 1:3 as the solvent and place it in the reboiler. Perform heating reflux extraction at 55-60 °C for 1-1.5 h, and collect the extract in the reboiler.

[0016] Fifth stage: Mix dichloromethane and ether in a volume ratio of 1:6 as the solvent and place it in the reboiler. Perform heating reflux extraction at 55-60 °C for 1-1.5 h, and collect the extract in the reboiler.

[0017] Sixth stage: Mix dichloromethane and ether in a volume ratio of 1:9 as the solvent and place it in the reboiler. Perform heating reflux extraction at 55-60 °C for 1-1.5 h, and collect the extract in the reboiler.

[0018] Furthermore, in each stage, the volume ratio of the mixed solvent of dichloromethane and ether used to the weight of the freeze-dried powder is 95-100 L:8 kg.

[0019] Further, in Step 1, pick fresh peonies and place them in a freezer. After freezing at -18°C for 10 - 15 h, transfer them to a freeze dryer and continuously dry them for 16 - 18 h under vacuum conditions at a temperature of -26 to -18°C and a pressure of 10 - 50 Pa to obtain dehydrated petal dry matter. Transfer the petal dry matter to a cold mill and continuously grind it at -10°C for 2 - 3 h, then pass through a 18 - 20 mesh sieve to obtain freeze-dried and pulverized matter.

[0020] Further, in Step 3, combine the extraction liquids obtained by staged extraction, heat at 55 - 60°C, and perform atmospheric distillation to recover dichloromethane and ether. After distilling until no more drops, add anhydrous ethanol to the distillation residue, perform atmospheric distillation at an external temperature of 85 - 90°C until no more drops, cool to room temperature, and then purge with nitrogen until dry to displace the original extraction solvent and remove the residual ethanol to obtain peony flower extraction paste.

[0021] On the other hand, the present invention proposes the application of the peony flower extraction paste obtained by the above-mentioned extraction process of peony flowers in the preparation of perfume.

[0022] A method for preparing perfume, using the peony flower extraction paste obtained by the above-mentioned extraction process of peony flowers. The method for preparing perfume includes the following steps: Transfer the peony flower extraction paste into a perfume primary production tank, inject distilled water 40 - 50 times the weight of the extraction paste, heat to 75 - 85°C, and extract the aromatic substances in the extraction paste into water with the assistance of ultrasonic waves; after ultrasonic extraction for 30 - 40 min, filter while it is hot. After the filtrate cools to room temperature, let it stand for stratification to obtain the supernatant. While stirring, add polyethylene glycol and sorbic acid to the supernatant, then add phenoxyethanol and glycerin, stir well, and filter through a PP membrane with a pore size of 0.45 μm to obtain a clear aqueous solution, which is the perfume with the fragrance of peony flowers.

[0023] Further, the dosage ratio of peony flower extraction paste, polyethylene glycol, sorbitol, phenoxyethanol, and glycerin is 100:0.1:0.05:1:3.

[0024] Further, during ultrasonic extraction, the ultrasonic power is 2000 - 2500 W, and the ultrasonic frequency is 19.5 - 20.5 KHz.

[0025] Compared with the prior art, the extraction process and application of the peony flowers of the present invention have the following advantages:

[0026] (1) Select a mixed solvent of dichloromethane and ether for heating and reflux extraction, which can be carried out at a lower temperature, reduce the loss of low-boiling components caused by a higher extraction temperature, and do not damage the components of the peony flower extract.

[0027] (2) The phased extraction method is adopted, and the volume ratio of dichloromethane and ether is continuously changed, so that components with smaller polarity and larger polarity can be better extracted, thereby improving the final total extraction efficiency.

[0028] (3) In the present invention, after the fresh flower petals are processed to obtain a freeze-dried and crushed product, extraction is carried out. During extraction, less solvent is required, the extraction time is shorter, the extraction completeness is higher, and it is not affected by environmental temperature and humidity.

[0029] (4) Ethanol is removed directly by nitrogen purging, which can avoid high-temperature vacuum distillation, avoid side reactions such as re-condensation or coking of the components of the peony flower extraction paste, and effectively retain the active ingredients of the peony flower.

[0030] (5) After the peony flower extraction paste obtained by the extraction process of the peony flower of the present invention is prepared into perfume, the fragrance can be smelled at room temperature, and the fragrance can fully restore the floral fragrance of the peony flower, reflecting the full fragrance of the peony flower. Specific embodiments

[0031] The present invention will be further described below in conjunction with specific embodiments. First, it should be noted that the data in the following experimental examples are obtained by the inventor through a large number of experiments. Due to space limitations, only a part of them are shown in the specification, and those of ordinary skill in the art can understand and implement the present invention based on this data. These embodiments are only used to illustrate the present invention and not to limit the scope of the present invention. In addition, it should be understood that after reading the content of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these changes or modifications also fall within the scope protected by the present invention.

[0032] An extraction process for peony flowers of the present invention includes the following steps:

[0033] Step 1: Pick fresh peony flowers, grind them through an 18-20 mesh sieve after freeze-drying to obtain a freeze-dried and crushed product;

[0034] Step 2: Use a Soxhlet extraction device for extraction. Place the freeze-dried and crushed product obtained in Step 1 in the extraction cell, add dichloromethane and ether as solvents, and carry out heating reflux extraction at 55-60 °C;

[0035] Step 3: After the extraction is completed, collect the extract in the reboiler, recover dichloromethane and ether to obtain the peony flower extraction paste.

[0036] Specifically, when adding dichloromethane and ether as solvents for heating reflux extraction in Step 2, a phased extraction method is adopted, specifically as follows:

[0037] In the first stage, dichloromethane and ether are mixed in a volume ratio of 6:1 as a solvent and placed in a reboiling kettle, heated under reflux extraction at 55-60° C. for 1-1.5 hours, and the extract in the reboiling kettle is collected;

[0038] In the second stage, dichloromethane and ether are mixed in a volume ratio of 3:1 as a solvent and placed in a reboiling kettle, heated under reflux extraction at 55-60° C. for 1-1.5 hours, and the extract in the reboiling kettle is collected;

[0039] In the third stage, dichloromethane and ether are mixed in a volume ratio of 1:1 as a solvent and placed in a reboiling kettle, heated under reflux extraction at 55-60° C. for 1-1.5 hours, and the extract in the reboiling kettle is collected;

[0040] In the fourth stage, dichloromethane and ether are mixed in a volume ratio of 1:3 as a solvent and placed in a reboiling kettle, heated under reflux extraction at 55-60° C. for 1-1.5 hours, and the extract in the reboiling kettle is collected;

[0041] In the fifth stage, dichloromethane and ether are mixed in a volume ratio of 1:6 as a solvent and placed in a reboiling kettle, heated under reflux extraction at 55-60° C. for 1-1.5 hours, and the extract in the reboiling kettle is collected;

[0042] In the sixth stage, dichloromethane and ether are mixed in a volume ratio of 1:9 as a solvent and placed in a reboiling kettle, heated under reflux extraction at 55-60° C. for 1-1.5 hours, and the extract in the reboiling kettle is collected;

[0043] Combine the above extracts and carry out the operation of step three.

[0044] More specifically, the ratio of the volume of the mixed solvent of dichloromethane and ether used in each stage to the weight of the freeze-dried powder is 95-100 L:8 kg.

[0045] When the present invention uses a Soxhlet extraction device to extract peony flowers, a mixed solvent of dichloromethane and ether is used for heating reflux extraction, and the extraction can be performed at a temperature of 55 to 60° C., thereby reducing the loss of low-boiling-point components caused by a higher extraction temperature and not destroying the components of the peony flower extract. Low-temperature extraction effectively retains all fractions from low to high, and the extracted peony flower effective components are prepared into perfume with a complex and comprehensive fragrance, and the fragrance fits the fragrance of fresh peony flowers. The fragrance smelled from fresh peony flowers is reproduced in the form of the "full fragrance" perfume, which better retains the original fragrance of fresh peony flowers, makes people feel happy, and has a comfortable feeling close to nature.

[0046] When using a mixed solvent of dichloromethane and ether for heating under reflux extraction, the present invention adopts a staged extraction method, continuously changing the volume ratio of dichloromethane and ether. In the initial stage, components with relatively low polarity can be extracted more, and by gradually increasing the proportion of ether, components with relatively high polarity can also be better extracted, thereby improving the final total extraction efficiency. The extracted peony flower extract is heated to obtain peony essential oil. By adopting the extraction process of peony flowers of the present invention, peony flowers with a short flowering period can be stored for a long time in the form of peony essential oil, which not only ensures the quality of peony essential oil but also improves the yield of peony essential oil.

[0047] Specifically, in step one, pick fresh peony flowers, place them in a freezer, freeze them at -18°C for 10 - 15 h, then transfer them to a freeze dryer, and continuously dry them at a temperature of -26 to -18°C and a vacuum condition of 10 - 50 Pa for 16 - 18 h to obtain dehydrated petal dry matter; transfer the petal dry matter to a cold mill, continuously grind it at a temperature of -10°C for 2 - 3 h, and pass through a 18 - 20 mesh sieve to obtain freeze-dried and pulverized matter.

[0048] In the present invention, the fresh petals are processed to obtain freeze-dried and pulverized matter before extraction. Compared with directly using fresh peony flowers, fresh peony petals contain a large amount of water, resulting in low extraction efficiency. After being prepared into freeze-dried and pulverized matter, since it is anhydrous, less solvent is required during extraction, the extraction time is shorter, and the extraction completeness is higher. At the same time, because the freeze-drying process is carried out under low-temperature conditions, it will not cause the loss of active ingredients during the drying process, and can more completely retain the active ingredients in peony flowers. Compared with directly using sun-dried peony petals, the sun-drying or air-drying process is in the air, diffusing from the high-humidity area in the peony petals to the low-humidity area in the air, and is greatly affected by environmental temperature and humidity. For different temperatures and humidities, the drying time, drying degree, and wet water content vary greatly. And because it is dried in an oxygen atmosphere, the peony petals will undergo varying degrees of mildew or even rot, and the extracted essential oil will deteriorate due to oxidation by oxygen. The freeze-drying process of the present invention is carried out in an almost vacuum state, with a fast water loss rate, no oxygen present, and a very low temperature, so there will be no mildew or rot.

[0049] Specifically, in step three, combine the extraction liquids obtained from the staged extraction, heat them at 55 - 60°C, perform atmospheric distillation to recover dichloromethane and ether. After distilling until no more drops, add anhydrous ethanol to the distillation residue, keep the external temperature at 85 - 90°C, perform atmospheric distillation until no more drops, cool to room temperature, and then purge with nitrogen until dry to displace the original extraction solvent and remove the residual ethanol to obtain peony flower extract. The recovered dichloromethane and ether can be reused, greatly reducing the cost. And the subsequent processes can effectively remove the original extraction solvent, with low solvent residue and not detected.

[0050] When purging to dryness with nitrogen, the nitrogen gas outlet is inserted below the liquid level, and the blowing speed is slow first and then fast. Removing ethanol by directly purging with nitrogen can avoid high-temperature vacuum distillation, prevent side reactions such as re-condensation or coking of the components in the peony flower extract paste, and effectively retain the active ingredients of the peony flower.

[0051] The extraction tank and reboiler described in the present invention are conventional components of a Soxhlet extraction device. The reboiler is used to re-evaporate the solvent containing the extract, and the evaporated solvent is used for re-extraction.

[0052] The present invention also proposes an application of the peony flower extract paste obtained by the above-mentioned peony flower extraction process in the preparation of a perfume with the fragrance of peony flower.

[0053] The preparation method of the perfume includes the following steps: Transfer the obtained peony flower extract paste into a perfume primary production tank, inject distilled water 40 - 50 times the weight of the extract paste, heat to 75 - 85 °C, and extract the aromatic substances in the extract paste into water with the assistance of ultrasonic waves; after ultrasonic extraction for 30 - 40 min, filter while it is hot. After the filtrate cools to room temperature, let it stand for stratification to obtain the supernatant. While stirring, add polyethylene glycol and sorbic acid to the supernatant, then add phenoxyethanol and glycerol, stir well, and filter through a PP membrane with a pore size of 0.45 μm to obtain a clear aqueous solution, which is the perfume with the fragrance of peony flower.

[0054] Specifically, the dosage ratio of the peony flower extract paste, polyethylene glycol, sorbitol, phenoxyethanol, and glycerol is 100:0.1:0.05:1:3.

[0055] Specifically, during ultrasonic extraction, the ultrasonic power is 2000 - 2500 W, and the ultrasonic frequency is 19.5 - 20.5 KHz.

[0056] Example 1

[0057] Pick 100 kg of fresh "Zhao Fen" peony flowers that have not yet fully opened to the initial blooming stage before 6:00 in the morning, wash them with water, and remove the flower stalks, receptacles, stamens, and pistils to obtain pure peony petals. After the peony petals are coarsely crushed, they are placed in a freezer at -18 °C for 10 h, then transferred to a freeze dryer and continuously dried for 16 h at -20 °C and 30 Pa to obtain dehydrated petal dry matter. Transfer the dehydrated petal dry matter to a cold mill and continuously grind it at -10 °C for 2 h, and pass through a 18-mesh sieve to obtain 16 kg of freeze-dried and pulverized matter.

[0058] Use a Soxhlet extraction device for extraction. Place the freeze-dried and pulverized matter in the extraction tank and perform staged heating and reflux extraction with a mixed solvent of dichloromethane and ether. Specifically, it includes the following steps:

[0059] In the first stage, 192 L of a mixed solvent of dichloromethane and ether was measured and placed in a reboiler. The volume ratio of dichloromethane to ether was 6:1, and it was heated under reflux at 55 - 60 °C for 1 h. The extract in the reboiler was collected;

[0060] In the second stage, 192 L of a mixed solvent of dichloromethane and ether was measured and placed in a reboiler. The volume ratio of dichloromethane to ether was 3:1, and it was heated under reflux at 55 - 60 °C for 1 h. The extract in the reboiler was collected;

[0061] In the third stage, 192 L of a mixed solvent of dichloromethane and ether was measured and placed in a reboiler. The volume ratio of dichloromethane to ether was 1:1, and it was heated under reflux at 55 - 60 °C for 1 h. The extract in the reboiler was collected;

[0062] In the fourth stage, 192 L of a mixed solvent of dichloromethane and ether was measured and placed in a reboiler. The volume ratio of dichloromethane to ether was 1:3, and it was heated under reflux at 55 - 60 °C for 1 h. The extract in the reboiler was collected;

[0063] In the fifth stage, 192 L of a mixed solvent of dichloromethane and ether was measured and placed in a reboiler. The volume ratio of dichloromethane to ether was 1:6, and it was heated under reflux at 55 - 60 °C for 1 h. The extract in the reboiler was collected;

[0064] In the sixth stage, 192 L of a mixed solvent of dichloromethane and ether was measured and placed in a reboiler. The volume ratio of dichloromethane to ether was 1:9, and it was heated under reflux at 55 - 60 °C for 1 h. The extract in the reboiler was collected.

[0065] The extracts from the above six stages were combined, heated at 55 - 60 °C, and distilled under normal pressure to recover dichloromethane and ether. After distilling until no more drops were obtained, 10 L of absolute ethanol was added to the distillation residue. With an external temperature of 90 °C, it was distilled under normal pressure until no more drops were obtained, cooled to room temperature, and then purged with nitrogen until dry to displace the original extraction solvent and remove the residual ethanol, obtaining 3.4 kg of peony flower extract paste.

[0066] Take 1 g of the peony flower extract paste and add 10 mL of ethanol at 75 °C to dissolve the extract paste, filter, and test the filtrate by GC - MS. The results are shown in Table 1.

[0067] Table 1

[0068]

[0069]

[0070]

[0071]

[0072] As can be seen from Table 1, the extraction paste obtained by using the extraction process of a kind of peony flower of the present invention has a relatively high content of components in the low molecular weight and low boiling point regions. At the same time, both the components in the high boiling point region and the low boiling point region are covered and have a relatively high content, which is conducive to maximizing the retention of the active ingredients in the peony flower. The peony flower extract contains alcohols, alkanes, esters, ketones, aromatic hydrocarbons and their derivatives, aldehydes, pyrans, alkenes, phenols, etc., so that the active components with different polarities are all extracted.

[0073] Transfer 3.4 kg of the extraction paste into the perfume primary production tank, inject distilled water 40 times the weight of the extraction paste, heat to 80 °C, and extract the aromatic substances in the extraction paste into the water with the assistance of ultrasonic waves. The ultrasonic power is 2000 W and the ultrasonic frequency is 20 KHz.

[0074] After ultrasonic extraction for 30 min, filter while it is hot. After the filtrate cools to room temperature, let it stand for stratification to obtain the supernatant. While stirring, add 3.4 g of polyethylene glycol and 1.7 g of sorbitol to the supernatant, then add 34 g of phenoxyethanol and 102 g of glycerin, stir well, and filter through a PP membrane with a pore size of 0.45 μm to obtain a clear aqueous solution, which is the perfume with the fragrance of peony flower. The prepared perfume can fully embody the full fragrance of the peony flower.

[0075] Comparative Example 1

[0076] In Comparative Example 1, the preparation of the freeze-dried and pulverized product was the same as in Example 1. The freeze-dried and pulverized product of the peony flower was extracted by the steam distillation method, and the peony flower extraction paste was obtained by vacuum distillation under the conditions of 120 °C and a vacuum degree of 35 Pa. The method for preparing the perfume from the peony flower extraction paste was the same as that in Example 1.

[0077] Comparative Example 2

[0078] In Comparative Example 2, the preparation of the freeze-dried and pulverized product was the same as in Example 1. When using dichloromethane and ether as a mixed solvent for heating and reflux extraction, dichloromethane and ether were mixed in a volume ratio of 6:1 for 1152 L and heated and refluxed for 9 h, and the extract was collected. The methods for treating the extract to obtain the peony flower extraction paste and perfume were the same as in Example 1.

[0079] In Comparative Example 1, when extracting peonies, due to the use of a relatively high temperature, the loss of low-boiling components occurred, and it was impossible to ensure that all components in the full series from low-boiling to high-boiling could be extracted and retained. The perfume prepared had basically no fragrance after use, and it was necessary to raise the temperature to above 50 °C to smell a faint fragrance, which was not conducive to daily use. In Comparative Example 2, when extracting peonies, the extraction of active components with relatively high polarity was limited, which not only caused a decrease in the yield of the extracted paste, but also led to a large deviation in the fragrance of the prepared perfume from that of peonies. However, the perfume of Example 1 could smell fragrant at room temperature, and the fragrance could restore the floral fragrance of peonies. The peony perfume has the advantages of anti-allergy and no irritation.

[0080] Although the present invention is disclosed as above, the present invention is not limited thereto. Any person skilled in the art can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention should be subject to the scope defined by the claims.

Claims

1. A peony flower extraction process, characterized in that: The steps include: Step 1, picking fresh peony flowers, grinding them through a 18-20 mesh sieve after freeze drying, and obtaining freeze-dried powder; Step 2, extracting with a Soxhlet extraction apparatus, placing the freeze-dried powder obtained in step 1 in an extraction tank, adding dichloromethane and ether as solvents, and performing heating reflux extraction at 55-60° C.; Step 3: After the extraction is completed, the extract in the reboiling kettle is collected, and the dichloromethane and ether are recovered to obtain the peony flower extract paste.

2. The extraction process of peony flower according to claim 1, characterized in that: In step 2, when dichloromethane and ether are added as solvents for heating and reflux extraction, a staged extraction method is adopted, which is as follows: In the first stage, dichloromethane and ether are mixed in a volume ratio of 6:1 as a solvent and placed in a reboiling kettle, heated under reflux extraction at 55-60° C. for 1-1.5 hours, and the extract in the reboiling kettle is collected; In the second stage, dichloromethane and ether are mixed in a volume ratio of 3:1 as a solvent and placed in a reboiling kettle, heated under reflux extraction at 55-60° C. for 1-1.5 hours, and the extract in the reboiling kettle is collected; In the third stage, dichloromethane and ether are mixed in a volume ratio of 1:1 as a solvent and placed in a reboiling kettle, heated under reflux extraction at 55-60° C. for 1-1.5 hours, and the extract in the reboiling kettle is collected; In the fourth stage, dichloromethane and ether are mixed in a volume ratio of 1:3 as a solvent and placed in a reboiling kettle, heated under reflux extraction at 55-60° C. for 1-1.5 hours, and the extract in the reboiling kettle is collected; In the fifth stage, dichloromethane and ether are mixed in a volume ratio of 1:6 as a solvent and placed in a reboiling kettle, heated under reflux extraction at 55-60° C. for 1-1.5 hours, and the extract in the reboiling kettle is collected; In the sixth stage, dichloromethane and ether are mixed in a volume ratio of 1:9 as a solvent and placed in a reboiling kettle. The mixture is heated under reflux extraction at 55-60° C. for 1-1.5 hours, and the extract in the reboiling kettle is collected.

3. The extraction process of peony flower according to claim 2, characterized in that: The ratio of the volume of the mixed solvent of dichloromethane and ether used in each stage to the weight of the freeze-dried powder is 95-100 L:8 kg.

4. The extraction process of peony flower according to claim 1, characterized in that: In step 1, fresh peony flowers are picked, placed in a freezing chamber, frozen at -18°C for 10-15 hours, and then transferred to a freeze dryer for continuous drying at a temperature of -26--18°C and a vacuum condition of 10-50 Pa for 16-18 hours to obtain dehydrated petal dried material; the petal dried material is transferred to a cold grinder, continuously ground at a temperature of -10°C for 2-3 hours, and passed through a 18-20 mesh sieve to obtain a freeze-dried crushed material.

5. The extraction process of peony flower according to claim 2, characterized in that: In step three, the extracts obtained by staged extraction are combined, heated at 55-60° C., distilled at normal pressure, distilled to recover dichloromethane and ether, and distilled until no dripping occurs. Anhydrous ethanol is added to the distillation residue, the external temperature is 85-90° C., distilled at normal pressure, distilled until no dripping occurs, cooled to room temperature, and then purged with nitrogen to dryness to replace the original extraction solvent, remove residual ethanol, and obtain a peony flower extract paste.

6. Use of the peony flower extract paste obtained by the peony flower extraction process as claimed in any one of claims 1 to 5 in preparing perfume.

7. A method for preparing perfume, characterized in that: The peony flower extract paste is obtained by extracting the peony flower extraction process according to any one of claims 1 to 5, and the preparation method of the perfume comprises the following steps: transferring the peony flower extract paste into a primary perfume preparation tank, injecting distilled water 40 to 50 times the weight of the extract paste, heating to 75 to 85°C, and extracting the aromatic substances in the extract paste into the water with the assistance of ultrasound; after ultrasonic extraction for 30 to 40 minutes, filtering while hot, letting the filtrate stand for stratification after cooling to room temperature to obtain a supernatant, adding polyethylene glycol and sorbic acid to the supernatant while stirring, and then adding phenoxyethanol and glycerol, stirring thoroughly and evenly, filtering through a PP membrane with a pore size of 0.45 μm to obtain a clear aqueous solution, which is the perfume with the fragrance of peony flowers.

8. The method for preparing perfume according to claim 7, characterized in that: The dosage ratio of peony flower extract, polyethylene glycol, sorbitol, phenoxyethanol and glycerin is 100:0.1:0.05:1:

3.

9. The method for preparing perfume according to claim 7, characterized in that: During ultrasonic extraction, the ultrasonic power is 2000-2500W and the ultrasonic frequency is 19.5-20.5KHz.

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