Detection liquid sample sampling equipment for lonicera caerulea gene sequencing

Abstract

The invention provides detection liquid sample sampling equipment for lonicera caerulea gene sequencing, and relates to the technical field of sample sampling. The device comprises a base, a supporting column, a sampling arm, a control panel and a cleaning device, the control panel is provided with operation buttons and a display screen which are used for controlling a sampling head to move, a suction device to work and the cleaning device to work, automatic sampling and cleaning operation of the device is achieved through a preset program, and the sampling arm is provided with the sampling head capable of moving up and down. The sampling head is connected with a suction device for extracting a liquid sample, and a sample placing table for placing a lonicera caerulea liquid sample container is arranged on the base. By means of automatic control and precise structural design, high-precision liquid sample sampling can be achieved, cross contamination of samples is effectively avoided, and the sample quality is improved; meanwhile, due to the replaceable sampling head, the flexible driving mechanism and the automatic cleaning device, the strict requirements of lonicera caerulea gene sequencing on high-quality samples are met.

Description

Technical Field

[0001] The present invention relates to a liquid sample sampling device for detecting blue loquat fruit gene sequencing, and in particular to sample sampling. Background Art

[0002] As a plant of significant economic and scientific value, genome sequencing of the blue indigo fruit provides insights into its genetic characteristics, growth and development mechanisms, and the molecular basis of its environmental adaptation. This is crucial for improving blue indigo varieties, controlling pests and diseases, and rationally developing and utilizing resources. During blue indigo fruit genome sequencing, obtaining high-quality test fluid samples is crucial for ensuring the accuracy and reliability of sequencing results.

[0003] The prior art discloses a device for sampling liquid samples for testing fruits and vegetables with application number: 202410651969.9, comprising a watertight pipe, the watertight pipe comprising a hollow pipe, the bottom end of the hollow pipe being connected to a rubber sleeve, a matching rubber pad being provided below the rubber sleeve, and a first spring being connected between the rubber sleeve and the rubber pad, the top end of the hollow pipe being fixedly connected to a platform and a handle, and a clamping assembly being mounted on the platform. The length of the sampling assembly is adjusted by the extension and contraction of the first electric push rod to drive the bellows to extend and contract, thereby facilitating a rough adjustment of the amount of sampling and ensuring the sampling sealing of the sampling assembly. An airbag is provided at the connection of the sampling assembly and the airbag is inflated through an air inlet pipe, so that the airbag expands and blocks the sampling assembly, facilitating the separation of liquids at different depths during the drug collection process, sampling liquids at different depths at one time, avoiding inconsistent sampling times for multiple times, facilitating liquid testing, and ensuring the representativeness of the sample. While the aforementioned patents enable single-use sampling of liquids at varying depths, ensuring sample representativeness, they suffer from insufficient sampling accuracy, making it difficult to accurately obtain high-quality samples that meet the requirements for gene sequencing of the blue indigo fruit. Furthermore, they fail to effectively prevent cross-contamination between samples, which affects the accuracy of sequencing results. Furthermore, the equipment has limited automation, resulting in cumbersome operation procedures and low sampling and cleaning efficiency. Furthermore, the sampling head has a single specification and is unable to adapt to the sampling requirements of different types of blue indigo fruit liquid samples, resulting in poor applicability. Therefore, a pressing technical issue that needs to be addressed is how to innovatively propose an effective approach to meet the needs of practical applications. Summary of the Invention

[0004] In response to the shortcomings of the above problems, the present invention provides a liquid sample sampling device for blue honeysuckle gene sequencing, which can achieve high-precision liquid sample sampling through automated control and precise structural design, effectively avoid sample cross-contamination, and improve sample quality; at the same time, through a replaceable sampling head, a flexible drive mechanism, and an automatic cleaning device, the applicability and working efficiency of the device are improved, meeting the strict requirements of blue honeysuckle gene sequencing for high-quality samples.

[0005] To achieve this object, the present invention adopts the following technical solutions:

[0006] A liquid sample sampling device for gene sequencing of blue loquat fruit comprises a base, a support column arranged on the base, and a sampling arm installed on the top of the support column. The device also includes a control panel and a cleaning device. The control panel is provided with operation buttons and a display screen for controlling the movement of a sampling head, the operation of a suction device, and the operation of a cleaning device. The display screen is used to display the working status and parameters of the device. A controller is provided within the control panel. The controller is electrically connected to a drive mechanism, the suction device, the cleaning device, and the display screen. Automatic sampling and cleaning operations of the device are achieved through a preset program. The sampling arm is provided with a sampling head that can move up and down. The sampling head is connected to the suction device for extracting a liquid sample. The base is provided with a sample placement table for placing a blue loquat fruit liquid sample container.

[0007] As a further technical solution of the present invention, the suction device includes a suction pipe connected to the sampling head and a micro pump arranged on the suction pipe, and the micro pump is controlled to be turned on and off by a control circuit.

[0008] As a further technical solution of the present invention, the sampling head is a detachable structure, which is connected to the suction pipe by a threaded connection or a snap connection, so as to facilitate the replacement of sampling heads of different specifications.

[0009] As a further technical solution of the present invention, the sampling arm is provided with a driving mechanism for driving the sampling head to move up and down. The driving mechanism includes a motor, a screw and a slider. The motor drives the screw to rotate. The slider is threaded with the screw and fixedly connected to the sampling head.

[0010] As a further technical solution of the present invention, a guide rod is provided on the outside of the screw rod, and a guide hole is provided on the slider to cooperate with the guide rod to ensure the stability of the up and down movement of the sampling head.

[0011] As a further technical solution of the present invention, the sample placement table is provided with a plurality of positioning grooves for accurately placing the blue loquat liquid sample container, and the shape and size of the positioning grooves are adapted to the sample container.

[0012] As a further technical solution of the present invention, the cleaning device includes a cleaning liquid storage tank, a cleaning pipeline and a nozzle. The nozzle is arranged near the sampling head and is used to clean the sampling head.

[0013] As a further technical solution of the present invention, a control valve is provided on the cleaning pipeline.

[0014] Beneficial effects of the present invention:

[0015] 1. High-precision automated sampling: The control panel and pre-set program precisely control the coordinated operation of the drive mechanism and suction device. A motor-driven screw drives the slider and sampling head for stable up and down movement. A micropump precisely controls the suction force, allowing liquid samples from blue locust fruit to be collected at a set depth and volume. This significantly improves sampling accuracy compared to existing technologies. Automated operation eliminates errors caused by manual intervention, ensuring the accuracy and consistency of each sample, providing a reliable sample foundation for genetic sequencing.

[0016] 2. Effectively prevent cross-contamination of samples: The detachable sampling head can be quickly cleaned after each sampling process using a cleaning device. The cleaning fluid in the cleaning fluid storage tank is regulated through the cleaning pipe and control valve, and the nozzle fully rinses the sampling head to completely remove residual samples. Furthermore, the sampling head's interchangeable design prevents residual interference between different samples, and the positioning slots on the sample placement table allow for precise container placement. This ensures sample purity throughout the entire process, from sampling to storage, effectively preventing cross-contamination and improving sample quality.

[0017] 3. High Applicability and Efficiency: A detachable sampling head with diverse specifications accommodates liquid samples of varying viscosities and compositions from blue loquats, meeting the needs of various sequencing experiments. The flexible adjustment and automated operation of the drive mechanism significantly shorten sampling time. An automatic cleaning device quickly cleans between sampling intervals, reducing waiting time and enabling continuous and efficient operation. Compared to existing technologies, this invention significantly improves the device's applicability and efficiency, reducing both experimental costs and time. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic structural diagram of the present invention;

[0019] Figure 2 It is a schematic diagram of the workflow of the present invention.

[0020] Description of main component symbols:

[0021] Base 1, support column 2, sampling arm 3, drive mechanism 4, motor 4a, screw 4b, slider 4c, sampling head 5, suction pipe 6, micro pump 7, control panel 8, cleaning device 9, display screen 8a, operation button 8b, cleaning device 9, cleaning liquid storage tank 9a, cleaning pipe 9b, nozzle 9c, open control valve 9d. DETAILED DESCRIPTION

[0022] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and examples, but the examples are not intended to limit the present invention.

[0023] like Figure 1-Figure 2As shown, an embodiment of the present invention includes a base 1, a support column 2 mounted on the base 1, and a sampling arm 3 mounted on top of the support column 2. The device also includes a control panel 8 and a cleaning device 9. The base 1 provides stable support for the entire device. The support column 2 is mounted on the base 1 to support the sampling arm 3, ensuring its stability and reliability. The sample placement platform 11 on the base 1 is equipped with multiple positioning grooves, the shape and size of which are compatible with the blue honeysuckle liquid sample container 10. These positioning grooves enable accurate placement of the sample container, ensuring that the sampling head 5 can accurately align with the sample for sampling.

[0024] (1) Sampling structure

[0025] Sampling arm 3 and sampling head 5: The sampling arm 3 is mounted on the top of the support column 2, and is equipped with a sampling head 5 that can move up and down. The sampling head 5 is a detachable structure, connected to the suction pipe 6 by a threaded connection or a snap connection, which facilitates the replacement of sampling heads 5 of different specifications according to different sampling needs.

[0026] Drive mechanism 4: Drive mechanism 4 is used to drive the sampling head 5 up and down. It comprises a motor 4a, a screw 4b, and a slider 4c. Motor 4a drives screw 4b, which is threaded into position and fixedly connected to the sampling head 5. A guide rod positioned outside screw 4b mates with a guide hole in slider 4c, ensuring the stability of the sampling head 5's vertical movement and enabling it to accurately reach the sample container for sampling.

[0027] Suction device: The suction device includes a suction pipe 6 connected to the sampling head 5 and a micropump 7 disposed on the suction pipe 6. The micropump 7 is turned on and off by a control circuit. When the micropump 7 is turned on, it can extract the blue indigo fruit liquid sample through the sampling head 5, thereby achieving sample collection.

[0028] (2) Control structure

[0029] Control panel 8: Control panel 8 is equipped with operating buttons 8b and a display screen 8a. Operating buttons 8b control the movement of the sampling head 5, the operation of the suction device, and the operation of the cleaning device 9. Display screen 8a displays the operating status and parameters of the device, such as the number of sampling times, the sampling volume, and the cleaning time. The controller within control panel 8 is electrically connected to the drive mechanism 4, the suction device, the cleaning device 9, and the display screen 8a. It implements automated sampling and cleaning operations through pre-set programs, improving the device's operational convenience and accuracy.

[0030] (3) Cleaning structure

[0031] Cleaning device 9: Cleaning device 9 includes a cleaning fluid storage tank 9a, a cleaning pipeline 9b, and a nozzle 9c. The nozzle 9c is located near the sampling head 5, and a control valve 9d is installed on the cleaning pipeline 9b. When the sampling head 5 needs to be cleaned, the control valve 9d is opened, and the cleaning fluid flows from the cleaning fluid storage tank 9a through the cleaning pipeline 9b to the nozzle 9c, cleaning the sampling head 5. This prevents cross-contamination between different samples and ensures sampling accuracy.

[0032] Workflow:

[0033] Preparation: Place the blue loquat fruit liquid sample container 10 accurately in the positioning slot of the sample placement table, select a sampling head 5 of appropriate specifications according to sampling requirements, and install it on the suction pipe 6.

[0034] Setting parameters: Sampling parameters, such as sampling volume, sampling times, etc., are set through the operation buttons 8b on the control panel 8, and the display screen 8a displays the set parameters.

[0035] Sampling Operation: The device is turned on. The controller activates motor 4a of drive mechanism 4 according to a preset program. Motor 4a drives screw 4b, causing slider 4c to move sampling head 5 downward into the sample container. The controller then activates micropump 7, extracting the blue loquat fruit liquid sample through sampling head 5. Once the extraction is complete, motor 4a reverses, causing sampling head 5 to return to its initial position.

[0036] Cleaning: After completing one or more sampling operations, the sampling head 5 needs to be cleaned to prevent cross contamination. The controller opens control valve 9d of the cleaning device 9. Cleaning fluid flows from the cleaning fluid storage tank 9a through the cleaning pipe 9b to the nozzle 9c, cleaning the sampling head 5. After cleaning is complete, the control valve 9d is closed.

[0037] The above description of the disclosed embodiments is intended to enable one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein but is intended to conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A liquid sample sampling device for detecting gene sequencing of blue loquat fruit, comprising a base (1), a support column (2) arranged on the base (1), and a sampling arm (3) installed on the top of the support column (2), characterized in that: The device further comprises a control panel (8) and a cleaning device (9). The control panel (8) is provided with operation buttons (8b) and a display screen (8a) for controlling the movement of the sampling head (5), the operation of the suction device and the operation of the cleaning device (9). The display screen (8a) is used to display the working status and parameters of the device. A controller is provided inside the control panel (8). The controller is electrically connected to the driving mechanism (4), the suction device, the cleaning device (9) and the display screen (8a). The automatic sampling and cleaning operations of the device are realized through a preset program. The sampling arm (3) is provided with a sampling head (5) that can move up and down. The sampling head (5) is connected to the suction device for extracting a liquid sample. The base (1) is provided with a sample placement table (11) for placing a blue indigo fruit liquid sample container (10).

2. The liquid sample sampling device for detecting gene sequencing of Lonicera edulis according to claim 1, characterized in that: The suction device comprises a suction pipe (6) connected to the sampling head (5) and a micro pump (7) arranged on the suction pipe (6); the micro pump (7) is controlled to be turned on and off by a control circuit.

3. The liquid sample sampling device for detecting gene sequencing of Lonicera edulis according to claim 2, characterized in that: The sampling head (5) is a detachable structure, connected to the suction pipe (6) by a threaded connection or a snap connection, so that the sampling head (5) of different specifications can be easily replaced.

4. The liquid sample sampling device for detecting gene sequencing of Lonicera edulis according to claim 1, characterized in that: The sampling arm (3) is provided with a driving mechanism (4) for driving the sampling head (5) to move up and down. The driving mechanism (4) comprises a motor (4a), a screw rod (4b) and a slider (4c). The motor (4a) drives the screw rod (4b) to rotate. The slider (4c) is threadedly engaged with the screw rod (4b) and is fixedly connected to the sampling head (5).

5. The liquid sample sampling device for detecting gene sequencing of Lonicera edulis according to claim 4, characterized in that: A guide rod is provided on the outside of the screw rod (4b), and a guide hole cooperating with the guide rod is provided on the slider (4c) to ensure the stability of the up and down movement of the sampling head (5).

6. The liquid sample sampling device for detecting gene sequencing of Lonicera edulis according to claim 1, characterized in that: The sample placement platform (11) is provided with a plurality of positioning grooves for accurately placing the blue loquat liquid sample container (10), and the shape and size of the positioning grooves are adapted to the sample container.

7. The liquid sample sampling device for detecting gene sequencing of Lonicera edulis according to claim 1, characterized in that: The cleaning device (9) comprises a cleaning liquid storage tank (9a), a cleaning pipeline (9b) and a nozzle (9c). The nozzle (9c) is arranged near the sampling head (5) and is used to clean the sampling head (5).

8. The liquid sample sampling device for detecting gene sequencing of Lonicera edulis according to claim 7, characterized in that: The cleaning pipeline (9b) is provided with a control valve (9d).

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