Extraction method of salvia miltiorrhiza-hawthorn medicine pair
By optimizing the extraction process of the Danshen-Hawthorn medicinal pair, using the ethanol reflux method and response surface methodology, and setting multiple index components as extraction standards, the problem of lack of standardization in the preparation process of the Danshen-Hawthorn medicinal pair was solved, the content and stability of the active ingredients in the extract were improved, and its modern development and clinical application were promoted.
Patent Information
- Application Number
- CN202510863665.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-06-26
- Publication Date
- 2025-09-26
AI Technical Summary
The preparation process of the Danshen-Crataegus herbal pair lacks a standardized scheme, resulting in insufficient depth in basic research, lack of scientific clinical application and limited market competitiveness. In addition, the complexity of the chemical composition makes it difficult to fully reflect the material basis of the efficacy through quantitative analysis of a single or single type of component.
Ethanol was used as the extraction solvent. The extraction process was optimized by single factor experiment and Box-Behnken response surface methodology. Tanshinone, chlorogenic acid, proanthocyanidin B2, epicatechin, hyperoside, isoquercetin, lithospermic acid, rosmarinic acid, salvianolic acid B, dihydrotanshinone I, cryptotanshinone and tanshinone IIA were set as extraction indicators. The reflux extraction process parameters were optimized to ensure that the content of active ingredients in the extract was not less than 8.0%.
It significantly increased the content of active ingredients in the extract of the Danshen-Hawthorn drug pair, improved the extraction efficiency and stability, provided technical support for modern development and clinical application, and enhanced market competitiveness.
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of traditional Chinese medicine extraction, and relates to a method for extracting a salvia miltiorrhiza-crataegus herb pair. Background Art
[0002] The Danshen-Hawthorn herbal pair, originating from Shi Jinmo's "Duiyao Dui", combines Danshen and Hawthorn, a classic clinical formula for treating ischemic heart disease (IHD) based on the traditional Chinese medicine theory of promoting blood circulation and removing blood stasis. Danshen promotes blood circulation and improves blood viscosity by promoting blood circulation and removing blood stasis, while Hawthorn promotes qi and blood stasis, clears turbidity and reduces lipids, promoting lipid metabolism and regulating dyslipidemia. The Danshen-Hawthorn combination enhances the effects of promoting qi and blood circulation, removing blood stasis and relieving pain, making it a classic combination for treating chest pain and heart pain caused by blood stasis.
[0003] In the development of modern preparations of traditional Chinese medicine, the optimization of the extraction process of active ingredients is a key technical bottleneck in promoting the industrialization of classical experimental directions. Currently, the Danshen-Hawthorn pair faces three development constraints due to the lack of a standardized preparation process: insufficient depth of basic research, lack of scientific clinical application, and limited market competitiveness. In addition, similar to most traditional Chinese medicine compounds, the chemical composition of the Danshen-Hawthorn pair is highly complex. The quantitative analysis of a single or single type of component is difficult to fully reflect the material basis of its efficacy. There is an urgent need to establish a comprehensive quality evaluation system based on the correlation of multiple indicators and components.
[0004] In summary, addressing the unique characteristics of the Danshen-Hawthorn herb pair and existing technical bottlenecks, developing a highly efficient extraction process based on a multi-index comprehensive evaluation standard will not only help improve the extraction efficiency and stability of the active ingredients, but also provide key technical support for the modern development, clinical application, and international market competition of this herb pair. The development and application of this technology also has important scientific value and industrial significance for achieving the development goal of Traditional Chinese Medicine (TCM) to "inherit the essence while maintaining integrity and innovation." Summary of the Invention
[0005] In order to solve the above technical problems, the present invention provides an extraction method for the Danshen-Hawthorn medicinal pair. The extraction method uses the total content of danshensu, chlorogenic acid, proanthocyanidin B2, epicatechin, hyperoside, isoquercetin, lithospermic acid, rosmarinic acid, salvianolic acid B, dihydrotanshinone I, cryptotanshinone and tanshinone IIA as the extraction index, and uses ethanol as the extraction solvent to reflux extract the Danshen-Hawthorn medicinal pair. The present invention optimizes the process parameters in the extraction method through single-factor experiments and Box-Behnken response surface experiments to obtain a Danshen-Hawthorn medicinal pair extract with an active ingredient content of not less than 8.0%. Based on a multi-index evaluation standard, the present invention provides a process with better extraction effect for the development and application of the Danshen-Hawthorn medicinal pair.
[0006] To achieve the technical objectives of the present invention, in one aspect, the present invention provides an extraction method for a Danshen-Hawthorn medicinal pair, comprising: extracting the Danshen-Hawthorn medicinal pair twice with 6-15 times the amount of 0-80% ethanol under reflux for 30-120 minutes each time, concentrating the mixture, placing it in a 25 mL volumetric flask, and adding 60% methanol to the mark. The solution is filtered through a 0.22 µm filter to obtain a test solution. The total content of danshensu, chlorogenic acid, procyanidin B2, epicatechin, hyperoside, isoquercitrin, lithospermic acid, rosmarinic acid, salvianolic acid B, dihydrotanshinone I, cryptotanshinone, and tanshinone IIA is used as an extraction index, and the total content of the above extraction indexes in the test solution is measured. The mass ratio of hawthorn to Danshen in the Danshen-Hawthorn medicinal pair is 1:1.
[0007] Preferably, the concentration of the ethanol is 70%, the reflux extraction time is 40 min, and the medicinal material is reflux-extracted with 15 times the amount of ethanol.
[0008] Specifically, the present invention determined through response surface analysis that the optimal extraction process was an ethanol concentration of 69.87%, a solid-liquid ratio of 1:14.77 (g / mL), and an extraction time of 39.37 minutes. Under these optimal conditions, the total content of 12 major compounds in the Danshen-Hawthorn extract was 8.386%. Based on practical feasibility, the optimal process parameters were selected as 70% ethanol concentration, a solid-liquid ratio of 1:15 (g / mL), and an extraction time of 40 minutes. To validate the accuracy of the model, three experiments were repeated under these conditions, resulting in a total content of 8.464% for the 12 major compounds in the Danshen-Hawthorn extract, with an RSD of 0.25%, close to the model's predicted value, demonstrating the stability, feasibility, and reproducibility of the conditions.
[0009] Specifically, the detection is performed using liquid chromatography, and the parameters of the liquid chromatography detection are: Column: Waters ACQUITY UPLC HSS T3 (2.1 mm × 100 mm, 1.8 μm); Flow rate: 0.3 mL·min -1 ; Column temperature: 40 °C; Detector: DAD detector; Detection wavelength: 280 nm; Mobile phase: acetonitrile (A)-0.1% phosphoric acid water (B), gradient elution; The gradient elution program was: 0-5 min, 5% mobile phase A; 5-18 min, 10% mobile phase A; 18-25 min, 35% mobile phase A; 25-30 min, 70% mobile phase A; 30-34 min, 90% mobile phase A; 34-34.1 min, 100% mobile phase A; 34.1-40 min, 5% mobile phase A.
[0010] On the other hand, the present invention claims protection for an extract of the Danshen-Hawthorn drug pair, prepared by the above-mentioned extraction method, wherein the active ingredients in the extract are danshensu, chlorogenic acid, proanthocyanidin B2, epicatechin, hyperoside, isoquercetin, lithospermic acid, rosmarinic acid, salvianolic acid B, dihydrotanshinone I, cryptotanshinone and tanshinone IIA; the content of the active ingredients in the extract is not less than 8.0%.
[0011] In addition, the present invention claims protection for a Danshen-crataegus medicinal preparation comprising the above extract and a pharmaceutically acceptable adjuvant.
[0012] Compared with the prior art, the technical solution provided by the present invention has at least the following beneficial effects or advantages: (1) The present invention provides a method for extracting the Danshen-Hawthorn medicinal pair, which is of great significance to the development and application of the Danshen-Hawthorn medicinal pair based on a multi-index comprehensive evaluation standard. The present invention uses an ethanol reflux method to extract the active ingredients in the Danshen-Hawthorn medicinal pair. The parameters of the extraction process are further optimized by single-factor experiments and Box-Behnken response surface method. The total content of danshensu, chlorogenic acid, proanthocyanidin B2, epicatechin, hyperoside, isoquercitrin, lithospermic acid, rosmarinic acid, salvianolic acid B, dihydrotanshinone I, cryptotanshinone and tanshinone IIA is used as an indicator to optimize the extraction method of the Danshen-Hawthorn medicinal pair with an active ingredient content of not less than 8.0%.
[0013] (2) The present invention determined the optimal extraction process to be 69.87% ethanol concentration, 1:14.77 (g / mL) solid-liquid ratio, and 39.37 min extraction time through response surface testing. Under the optimal process conditions, the total content of the 12 main compounds in the extract of the Danshen-Hawthorn pair was 8.386%. Combined with the feasibility of actual operation, 70% ethanol concentration, 1:15 (g / mL) solid-liquid ratio, and 40 min extraction time were selected as the optimal process parameters. To verify the accuracy of the model, the experiment was repeated three times under these conditions. The total content of the 12 main compounds in the extract of the Danshen-Hawthorn pair was 8.464%, with an RSD of 0.25%, which is close to the predicted value of the model, indicating that the conditions are stable, feasible, and reproducible.
[0014] (3) The present invention extracts Danshen and Crataegus pinnatifida simultaneously, which significantly increases the content of the active ingredients in the extract. When Danshen and Crataegus pinnatifida are extracted together, the active ingredient content is 160.068 mg / kg. When Danshen and Crataegus pinnatifida are extracted separately, the sum of the active ingredient contents is 132.064 mg / kg, which is significantly lower than the amount extracted when Danshen and Crataegus pinnatifida are extracted simultaneously. It can be seen that there is an interaction between the active ingredients of Danshen and Crataegus pinnatifida, and extracting them simultaneously can significantly improve the extraction effect. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention.
[0016] Figure 1 UHPLC chromatograms of different test samples. Figure 1 A in the figure is the UHPLC spectrum of hawthorn sample; Figure 1 B in the figure is the UHPLC spectrum of the Salvia miltiorrhiza sample; Figure 1 C in the figure is the UHPLC spectrum of the mixed reference substance 1 solution; Figure 1 D in the figure is the UHPLC spectrum of the mixed reference substance 2 solution; Figure 1 E in the figure is the UHPLC spectrum of the mixed reference substance 3 solution; Figure 1 Figure F is the UHPLC chromatogram of a 1:1 Danshen-Crataegus sample. The accompanying figures are labeled as follows: 1: Danshensu, 2: Chlorogenic acid, 3: Proanthocyanidin B2, 4: Epicatechin, 5: Hyperoside, 6: Isoquercitrin, 7: Lithospermic acid, 8: Rosmarinic acid, 9: Salvianolic acid B, 10: Dihydrotanshinone I, 11: Cryptotanshinone, and 12: Tanshinone IIA.
[0017] Figure 2 Response surface plot and contour plot. Figure 2 A in is the response surface diagram of ethanol concentration and solid-liquid ratio; Figure 2 B in the figure is the contour map of ethanol concentration and solid-liquid ratio; Figure 2 C in is the response surface diagram of ethanol concentration and extraction time; Figure 2 D in is the contour plot of ethanol concentration and extraction time; Figure 2 E in is the response surface diagram of solid-liquid ratio and extraction time; Figure 2 F is the contour diagram of solid-liquid ratio and extraction time. DETAILED DESCRIPTION
[0018] The technical solutions of the present invention are described below with reference to the following examples. However, the present invention is not limited to the following examples. The experimental methods and detection methods described in each example are conventional methods unless otherwise specified; the reagents and materials described are commercially available unless otherwise specified.
[0019] Salvia miltiorrhiza and Crataegus pinnatifida were purchased from Shaanxi Duoyao Chinese Medicine Pieces Co., Ltd. with batch numbers YD008-220501 and 230501, respectively.
[0020] Lithospermic acid, salvianolic acid B and danshensu were purchased from Baoji Chenguang Biotechnology Co., Ltd., with a content of 98%.
[0021] Dihydrotanshinone I was purchased from Shanghai Yuanye Biotechnology Co., Ltd. with a content of 98%.
[0022] Rosmarinic acid and chlorogenic acid were purchased from Shanghai Bid Pharmaceutical Technology Co., Ltd., with a content of 98%.
[0023] Cryptotanshinone, tanshinone IIA and hyperoside were purchased from Aifa Biotechnology Co., Ltd., with a content of 98%.
[0024] Isoquercetin was purchased from the China Food and Drug Inspection Institute with a content of 97.2%.
[0025] Proanthocyanidin B2 was purchased from Shanghai Tongtian Biotechnology Co., Ltd. with a content of 98%.
[0026] Anhydrous ethanol was purchased from Tianjin Fuyu Fine Chemical Co., Ltd., acetonitrile, phosphoric acid, and methanol were purchased from ThermoFisher, and all reagents were HPLC grade.
[0027] Example 1 This example provides an extraction method for the Danshen-Hawthorn medicinal pair and detection of the main chemical components of the test sample.
[0028] 1. Extraction method of Danshen-Hawthorn medicinal pair The chemical components of the Danshen-Hawthorn drug pair were extracted by ethanol reflux extraction. Danshen, hawthorn, and Danshen-Hawthorn 1:1 (the mass ratio of Danshen to hawthorn was 1:1) were weighed, with a total of 10 g each. The medicinal materials were reflux extracted twice with 8 times the amount of 80% ethanol, each time for 60 min, concentrated, placed in a 25 mL volumetric flask, and 60% methanol was added to the scale. The solution was filtered with a 0.22 µm filter membrane to obtain the test solution.
[0029] 2. Detection of the main chemical components of the test samples Preparation of reference solution: Accurately weigh an appropriate amount of each reference substance, dissolve in 50% methanol, and shake thoroughly. A mixed reference solution 1 containing 5.93 μg / mL of danshensu, 9.84 μg / mL of rosmarinic acid, 11.11 μg / mL of lithospermic acid, and 201.10 μg / mL of salvianolic acid B was prepared. Methanol was added to prepare a mixed reference solution 2 containing 1.33 μg / mL of dihydrotanshinone I, 8.46 μg / mL of cryptotanshinone, and 2.60 μg / mL of tanshinone IIA. A mixed reference solution 3 containing 5.13 μg / mL of chlorogenic acid, 4.53 μg / mL of epicatechin, 2.55 μg / mL of procyanidin B2, 4.46 μg / mL of hyperoside, and 8.47 μg / mL of isoquercetin was prepared by adding 50% methanol.
[0030] 2 μL of each of the mixed reference solution 1 to 3 and the test solution was accurately aspirated and injected into a high performance liquid chromatograph (Thermo Vanquish Flex UHPLC liquid chromatograph) for determination. The chromatographic column was Waters ACQUITY UPLCHSS T3 (2.1 mm × 100 mm, 1.8 μm). The chromatographic conditions were: flow rate 0.3 mL·min -1 Column temperature: 40°C; DAD detector: detection wavelengths: 256 nm, 280 nm, and 326 nm. Mobile phase: acetonitrile (A)-0.1% phosphoric acid (B). Gradient elution is shown in Table 1. Peak areas of the main chemical components were recorded, and the content of each component was calculated using the external standard method. The chromatographic peak information for each main chemical component is shown in Table 2.
[0031] Under the above chromatographic conditions, the baseline is stable, the retention time of the 12 main chemical components in the test solution is basically consistent with that of the reference substance, and the separation effect with adjacent chromatographic peaks is good. The theoretical plate number is not less than 5500 for each component. The chromatogram is as follows Figure 1 The main chemical components of the Danshen-Hawthorn herb pair (1:1) were analyzed at a chromatographic wavelength of 280 nm and compared with mixed standard solutions 1 to 3. The results showed that danshensu, chlorogenic acid, procyanidin B2, epicatechin, hyperoside, isoquercetin, lithospermic acid, rosmarinic acid, salvianolic acid B, dihydrotanshinone I, cryptotanshinone, and tanshinone IIA were detected in the Danshen sample; danshensu, lithospermic acid, rosmarinic acid, salvianolic acid B, dihydrotanshinone I, cryptotanshinone, and tanshinone IIA were detected in the Hawthorn sample; and chlorogenic acid, procyanidin B2, epicatechin, hyperoside, and isoquercetin were detected in the Hawthorn sample.
[0032] Table 1 Gradient elution table
[0033] Table 2 Chromatographic peak information of active ingredients
[0034] Example 2 This example provides process optimization of the extraction method of the Salvia miltiorrhiza-crataegi drug pair.
[0035] In the early stage of the experiment, it was found that there were many factors that affected the extraction effect. In the present invention, ethanol concentration, material-liquid ratio and extraction time were selected as the main influencing factors for single-factor experiments.
[0036] 1. Single factor test of the extraction method of Danshen-Hawthorn drug pair Single-factor experiment on ethanol concentration: The extraction method for the Danshen-Hawthorn herb pair was the same as in Example 1. The effects of different ethanol concentrations on the extraction efficiency of the Danshen-Hawthorn herb pair were compared (Table 3). As shown in Table 3, the total contents of the 12 major compounds in the Danshen-Hawthorn herb pair extracts were 4.834%, 6.581%, 7.383%, 7.797%, and 7.535% at ethanol concentrations of 0% (pure water), 20%, 40%, 60%, and 80%, respectively. The highest total content was achieved at 60% ethanol. Therefore, 60% ethanol was selected as the optimal process for the single-factor experiment on ethanol concentration.
[0037] Table 3 Effects of different ethanol concentrations on the extraction of Danshen-Hawthorn drug pair
[0038] Single-factor experiment on the ratio of medicinal material to 80% ethanol: The extraction method for the Danshen-Hawthorn herb pair was the same as in Example 1. The effects of different ratios on the extraction efficiency of the Danshen-Hawthorn herb pair were compared (Table 4). As shown in Table 4, the total contents of the 12 major compounds in the Danshen-Hawthorn herb pair extracts were 8.110%, 8.111%, 8.061%, 8.124%, and 8.468% at ratios of 1:6, 1:8, 1:10, 1:12, and 1:14 (g / mL), respectively. The highest total content was achieved at a ratio of 1:14. Therefore, a ratio of 1:14 was selected as the optimal process for the single-factor experiment on the ratio of medicinal material to 80% ethanol.
[0039] Table 4 Effects of different material-liquid ratios on the extraction effect of Danshen-Hawthorn drug pair
[0040] Single-factor experiment on extraction time: The extraction method for the Danshen-Hawthorn herb pair was the same as in Example 1. The effects of different extraction times on the extraction efficiency of the Danshen-Hawthorn herb pair were compared (Table 5). As shown in Table 5, the total contents of the 12 major compounds in the Danshen-Hawthorn herb pair extracts were 8.592%, 8.096%, 7.962%, and 7.899% for extraction times of 30, 60, 90, and 120 minutes, respectively. The highest total content was achieved with an extraction time of 30 minutes. Therefore, a 30-minute extraction time was selected as the optimal extraction time for the single-factor experiment.
[0041] Table 5 Effects of different extraction times on the extraction effect of Danshen-Hawthorn drug pair
[0042] 2. Response surface experiment of the extraction method of Danshen-Hawthorn drug pair (1) Box-Behnken test design and results Based on the single-factor analysis, ethanol concentration (A), solid-liquid ratio (B), and extraction time (C) were set as independent variables, and the total extraction yield of 12 major compounds in the Danshen-Hawthorn extract was used as the evaluation indicator. The effects of each factor on the extraction process were investigated, with three levels selected for each factor. The experimental design factor levels are shown in Table 6. Using the principles of Box-Behnken response surface methodology, a three-factor, three-level response surface analysis experiment (15 experimental points) was designed, with ethanol concentration, extraction time, and solid-liquid ratio as independent variables, including the total extraction yield of the 12 major compounds. The experimental design and results are detailed in Table 7.
[0043] Table 6 Box-Behnken response surface test factor level table
[0044] Table 7 Box-Behnken response surface method experimental design and results
[0045] (2) Model establishment and significance analysis Design expert 12 software was used to perform regression fitting and variance analysis on the total extraction amount of 12 main compounds in the extracts of Danshen-Hawthorn drug pair at 15 test points, and the polynomial regression equation Y=7.36+0.37A-0.34B-0.21C+0.25AB+0.66AC+0.30BC+0.11A was obtained. 2 -0.05B 2 +0.14C 2 , R 2=0.9467, indicating that the model fitting result is ideal. The specific variance analysis results are shown in Table 8. F The value is 23.70, P The value is 0.00011, and the lack of fit P The value > 0.05 indicates that the model has significant differences. P The values are all <0.05, indicating that the primary effects of these three factors have a significant impact on the response. F Value and P The results show that the extraction effect is affected by ethanol concentration, solid-liquid ratio, and extraction time in descending order: ethanol concentration > solid-liquid ratio > extraction time. Among the interactions of these process parameters, the interactions of ethanol concentration × solid-liquid ratio, ethanol concentration × extraction time, and solid-liquid ratio × extraction time are significant. P The values were all less than 0.05, indicating a significant effect on the response. The degree of interaction of the three factors on the response was in the order of ethanol concentration × extraction time > ethanol concentration × material-liquid ratio > material-liquid ratio × extraction time.
[0046] Table 8 Box-Behnken response surface method variance analysis results
[0047] (3) Response surface optimization results According to the fitting results, the response surface diagram and contour diagram were drawn using Design expert 12 software ( Figure 2 Combined with response surface model predictions, the optimal extraction process was determined to be an ethanol concentration of 69.87%, a solid-liquid ratio of 1:14.77 (g / mL), and an extraction time of 39.37 min. Under these optimal conditions, the total content of the 12 main compounds in the Danshen-Hawthorn extract was 8.386%. Considering practical feasibility, the optimal process parameters were selected as 70% ethanol concentration, a solid-liquid ratio of 1:15 (g / mL), and an extraction time of 40 min. To validate the accuracy of the model, three experiments were repeated under these conditions, resulting in a total content of 8.464% for the 12 main compounds in the Danshen-Hawthorn extract with an RSD of 0.25%, which is close to the model's prediction, indicating that the conditions are stable, feasible, and reproducible.
[0048] Comparative Example 1 In this comparative example, Danshen and Crataegus pinnatifida were extracted separately, and the extraction scheme was the same as the optimal extraction process obtained in Example 2. The components of Crataegus pinnatifida were procyanidin B2, epicatechin, hyperoside, isoquercetin and chlorogenic acid, and the components of Danshen were tanshinone, salvianolic acid B, rosmarinic acid, lithospermic acid, dihydrotanshinone I, cryptotanshinone and tanshinone IIA. The extraction effects of the active ingredients are shown in Table 9.
[0049] Table 9 Extraction effect of the extraction method of the Danshen-Hawthorn drug pair
[0050] As shown in Table 9, when Danshen and Crataegus pinnatifida were extracted together, the effective ingredient content was 160.068 mg / kg. After Danshen and Crataegus pinnatifida were extracted separately, the sum of the effective ingredient contents was 132.064 mg / kg, which was significantly lower than the extraction amount when Danshen and Crataegus pinnatifida were extracted at the same time. It can be seen that there is an interaction between the effective ingredients of Danshen and Crataegus pinnatifida, and extracting them simultaneously can significantly improve the extraction effect.
[0051] The embodiments described above are some of the embodiments of the present invention, rather than all of them. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but rather merely represents selected embodiments of the present invention. All other embodiments obtained without creative effort and through deduction and substitution by a person of ordinary skill in the art based on the concept of the present invention are within the scope of protection of the present invention.
Claims
1. A method for extracting a Salvia miltiorrhiza-crataegi drug pair, characterized in that: include: The active ingredients include danshensu, chlorogenic acid, proanthocyanidin B2, epicatechin, hyperoside, isoquercetin, lithospermic acid, rosmarinic acid, salvianolic acid B, dihydrotanshinone I, cryptotanshinone and tanshinone IIA; Using ethanol as the extraction solvent, the drug pair of Danshen and Crataegus pinnatifida is subjected to reflux extraction to obtain an extract; The total content of the active ingredients in the extract is detected.
2. The extraction method according to claim 1, wherein The concentration of the ethanol is 0-80%; The reflux extraction time is 30 to 120 min; The solid-liquid ratio of the Danshen-crataegus medicinal pair to ethanol is 1:6-1:15; The unit of the material-liquid ratio is g:mL.
3. The extraction method according to claim 2, characterized in that The concentration of the ethanol is 70%; The reflux extraction time is 40 min; The solid-liquid ratio of the salvia miltiorrhiza-crataegus medicinal pair to ethanol is 1:
15.
4. An extract of a Salvia miltiorrhiza-crataegi drug pair, characterized in that: The extract is prepared by the extraction method according to any one of claims 1 to 3.
5. The extract according to claim 4, characterized in that The content of active ingredients in the extract is not less than 8.0%; The effective ingredients are composed of tanshinone, chlorogenic acid, proanthocyanidin B2, epicatechin, hyperoside, isoquercetin, lithospermic acid, rosmarinic acid, salvianolic acid B, dihydrotanshinone I, cryptotanshinone and tanshinone II A.
6. A preparation comprising an extract of the Danshen-Hawthorn drug pair, characterized in that: The invention comprises the extract according to any one of claims 4 to 5 and a pharmaceutically acceptable auxiliary agent.