Deer blood and polygonatum kingianum wine for tonifying kidney and qi and enhancing immunity and preparation method of deer blood and polygonatum kingianum wine

Abstract

The invention provides deer blood and rhizoma polygonati wine for tonifying kidney and qi and enhancing immunity and a preparation method of the deer blood and rhizoma polygonati wine, and relates to the technical field of wine brewing. The deer blood and rhizoma polygonati wine is prepared from the following raw materials in parts by weight: 50 to 60 parts of deer blood extract, 50 to 60 parts of rhizoma polygonati extract, 30 to 40 parts of climbing fig extract, 10 to 20 parts of rubus buergeri root extract, 6 to 8 parts of oyster, 5 to 10 parts of folium cortex eucommiae, 5 to 10 parts of herba cistanche and 450 to 550 parts of 50-degree white spirit. On the basis of the deer blood extract and the rhizoma polygonati extract, the ficus pumila extract, the rubus buergeri root extract, the oysters, the folium cortex eucommiae and the herba cistanche are added into the 50-degree white spirit to prepare the deer blood and rhizoma polygonati wine, and the deer blood and rhizoma polygonati wine is mellow in mouth feel, outstanding in medicine fragrance, smooth and harmonious, and has taste, color and luster; meanwhile, all the raw materials contain rich functional components, can be directly absorbed by the human body without digestion, increase the absorption rate, reduce the burden of internal organs, nourish blood and essence, tonify qi and yin, restore pulse and relieve desertion, tonify spleen and lung, promote salivation and nourish blood, enhance immunity, prolong life after being taken for a long time, and have good application prospects.

Description

Technical Field

[0001] The invention relates to the technical field of winemaking, in particular to deer blood and polygonatum wine for tonifying the kidney and replenishing qi and enhancing immunity and a preparation method thereof. Background Art

[0002] Wine is known as the "king of all medicines". Medicinal wine that "dissolves" the health-strengthening traditional Chinese medicine with wine is not only easy to prepare, stable in medicinal properties, safe and effective, but also because alcohol is a good semi-polar organic solvent, various active ingredients of traditional Chinese medicine are easily soluble in it. The medicine uses the power of wine and the wine helps the medicine to fully exert its effectiveness and improve the efficacy.

[0003] Sika deer blood is a precious Chinese medicine. Since ancient times, it has been a treasure for the royal family, dignitaries and nobles to treat diseases and keep fit. At present, the main ways to consume sika deer blood are to make soup, braise it, etc., or to air-dry it and make it into pills or powders, or mix it with white wine to make medicinal wine. The characteristics of white wine that can promote blood circulation, disperse, be easily absorbed and promote medicinal properties, as well as people's daily habit of drinking a moderate amount of white wine, are used to achieve an organic combination of diet and health care. However, the sika deer blood wine currently sold on the market has a poor taste, unsatisfactory medicinal effect, and uneven quality.

[0004] Polygonatum sibiricum is a plant in the Liliaceae family. The Chinese Pharmacopoeia lists Polygonatum sibiricum, Polygonatum sibiricum, and Polygonatum multiflorum as examples. Functional research focuses on the extraction of active ingredients in Polygonatum sibiricum. Polygonatum sibiricum contains a rich variety of active ingredients, including polysaccharides, oligosaccharides, steroidal saponins, polyphenols, flavonoids, amino acids, and trace elements. Studies have shown that it possesses a variety of physiological functions, including antioxidant, anti-aging, hypoglycemic, anti-inflammatory, anti-tumor, memory-enhancing, anti-dementia, and immunomodulatory properties. Polygonatum sibiricum is a Chinese herbal medicine with both medicinal and edible properties and is currently widely used in the pharmaceutical and health care product industries.

[0005] How to mix deer blood and polygonatum to make the resulting wine product have herbal sweetness without fishy smell, have both taste and color, improve health care effects, and be suitable for mass consumption needs, which has practical significance. Summary of the Invention

[0006] In view of this, the present invention provides a deer blood and polygonatum wine for tonifying the kidney and replenishing qi and enhancing immunity, and a preparation method thereof.

[0007] The technical solution of the present invention is achieved as follows: A deer blood and polygonatum wine for nourishing the kidney and replenishing qi and enhancing immunity comprises the following raw materials, in parts by weight: 50-60 parts of deer blood extract, 50-60 parts of polygonatum extract, 30-40 parts of ficus pumila extract, 10-20 parts of berry root extract, 6-8 parts of oysters, 5-10 parts of eucommia leaves, 5-10 parts of cistanche deserticola, and 450-550 parts of 50-proof liquor.

[0008] Furthermore, the preparation method of the deer blood extract comprises: The sika deer antler blood is placed in a citric acid solution and shaken, and a complex enzyme is added for enzymatic hydrolysis, followed by ultrasonic treatment and centrifugation. The supernatant is collected and purified through an ultrafiltration membrane with a diameter of less than 1 kDa. The filtrate is freeze-dried to obtain a deer blood extract.

[0009] Furthermore, the mass ratio of the sika deer antler blood to the citric acid solution is 1:4-6; the concentration of the citric acid solution is 8-12 wt %; the oscillation temperature is 5-10° C., and the oscillation time is 10-15 min; The mass ratio of the complex enzyme to sika deer antler blood is 0.2-0.4:1; the complex enzyme comprises trypsin, lipase and Bacillus pumilus protease in a mass ratio of 3-5:1:2-4; the enzymatic hydrolysis temperature is 28-35°C and the time is 1-3h; The power of the ultrasonic treatment is 10-20 kHz, and the time is 10-20 min; the centrifugation is performed at 2500-3500 rpm for 5-10 min, and at 4000-5000 rpm for 6-8 min.

[0010] Furthermore, the preparation method of the polygonatum extract comprises: The polygonatum sibiricum is crushed and added into water, a composite enzyme is added for enzymolysis, and the mixture is centrifuged to obtain an enzymolysis solution and a residue. The residue is fermented with an activated composite bacterial strain to obtain a fermentation product, the fermentation product and the enzymolysis solution are combined, ethylenediaminetetraacetic acid and ascorbic acid are added for oscillation to obtain a mixed solution, the mixture is centrifuged, and the mixture is freeze-dried to obtain a polygonatum sibiricum extract.

[0011] Furthermore, the material-liquid ratio of polygonatum to water is 1:10-20 g / mL; the mass ratio of the complex enzyme to polygonatum is 0.15-0.3:1; the complex enzyme is xylanase, β-glucanase and hemicellulase in a mass ratio of 1-2:0.8-1.2:1; the enzymatic hydrolysis is carried out at 30-35° C. for 1-2 hours; The inoculation amount of the composite strain is 1%-3% of the mass of the residue; the composite strain is composed of Aspergillus niger, Trichosporon berberis, and Saccharomyces cerevisiae in a mass ratio of 3-4:1:1-2; the fermentation temperature is 28-32°C, and the fermentation time is 10-12 hours; The concentration of EDTA in the mixed solution is 2-3 mmol / L, and the concentration of ascorbic acid is 3-4 mmol / L; the oscillation time is 5-10 min.

[0012] Furthermore, the preparation method of the Ficus pumila extract comprises: The roots or fruits of Ficus pumila are crushed, placed in a supercritical CO2 extraction device for extraction, and the extract is concentrated under reduced pressure to obtain the Ficus pumila extract.

[0013] Furthermore, the supercritical CO2 extraction has a pressure of 20-30 MPa, a temperature of 40-50°C, and a time of 1-2 hours.

[0014] Furthermore, the preparation method of the berry root extract includes: The root of the winterberry is crushed, added into an ethanol solution, subjected to microwave heating extraction, and the extract is concentrated under reduced pressure to obtain the winterberry root extract.

[0015] Furthermore, the material-liquid ratio of the winterberry root to the ethanol solution is 1:8-12 g / mL; the concentration of the ethanol solution is 60-70% v / v; the power of the microwave heating extraction is 300-500 W, the temperature is 60-70° C., and the time is 40-60 min.

[0016] A method for preparing deer blood and polygonatum wine for tonifying the kidney and replenishing qi and enhancing immunity comprises the following specific preparation steps: soaking deer blood extract, polygonatum extract, ficus pumila extract, cyperus root extract, oyster, eucommia leaf and cistanche deserticola in 50% alcohol liquor, sealing and storing for 3-4 months, and filtering to obtain the target deer blood and polygonatum wine.

[0017] The deer blood of the present invention has the following functions: salty and warm in nature and flavor; and has the following functions and indications: tonifying deficiency, replenishing blood and benefiting essence; and is used for anemia, sexual neurasthenia and spermatorrhea.

[0018] Polygonatum: sweet, flat, non-toxic; functions and indications: nourishes the liver and improves eyesight, treats leprosy, spleen and stomach weakness, fatigue, replenishes qi and nourishes yin, strengthens the spleen, moistens the lungs, and benefits the kidneys.

[0019] Pseudostellaria baicalensis: sour and neutral in nature; functions and indications: dispelling wind and dampness, promoting blood circulation and unblocking meridians, detoxifying and reducing swelling; has the effect of strengthening yang and consolidating essence.

[0020] Cold berry root: acidic, flat, non-toxic; functions and indications: clearing away heat and detoxifying, promoting blood circulation and relieving pain; treating stomachache, hyperacidity, spermatorrhea, and headache.

[0021] Oyster: salty, neutral, slightly cold, non-toxic; Functions and indications: pain in the heart and spleen.

[0022] Eucommia ulmoides leaves: slightly pungent and warm in nature. Functions and indications: tonifies the liver and kidneys; strengthens bones and tendons; lowers blood pressure. Treats back pain; soreness and weakness in the feet and knees; and hypertension.

[0023] Cistanche deserticola: sweet, slightly warm, non-toxic; functions and indications: nourishes essence and blood, replenishes kidney yang, and promotes bowel movements and moistens the intestines.

[0024] Compared with the prior art, the present invention has the following beneficial effects: The deer blood and polygonatum sibiricum wine prepared by the present invention is based on deer blood extract and polygonatum sibiricum extract, and is added with phellodendron chinense extract, cyperus chinensis root extract, oyster, eucommia ulmoides leaf, and cistanche deserticola to 50-proof liquor. The wine has a mellow taste, a prominent medicinal aroma, a smooth and harmonious taste, and a good taste and color. At the same time, each raw material contains rich functional ingredients, which can be directly absorbed by the human body without digestion, thereby increasing the absorption rate, reducing the burden on internal organs, nourishing blood and essence, replenishing qi and nourishing yin, restoring pulse and strengthening deficiency, replenishing the spleen and lungs, promoting the production of body fluids and nourishing blood, enhancing immunity, and can prolong life by long-term use. The wine has good application prospects. DETAILED DESCRIPTION

[0025] In order to better understand the technical content of the present invention, specific examples are provided below to further illustrate the present invention.

[0026] Unless otherwise specified, the experimental methods used in the examples of the present invention are all conventional methods.

[0027] Unless otherwise specified, the materials, reagents, etc. used in the examples of the present invention can be obtained from commercial sources.

[0028] The sika deer antler blood of the present invention comes from a sika deer breeding base in Jilin Province, and is obtained from antlers of robust sika deer in estrus.

[0029] The polygonatum sibiricum of the present invention comes from the Chongyang polygonatum sibiricum planting base, one of the top ten Chu medicines in Hubei Province, and is selected from high-quality polygonatum sibiricum that is over 5 years old and planted in a simulated wild manner.

[0030] The eucommia leaves of the invention are from a eucommia leaf planting base in Jilin, and are selected from high-quality eucommia leaves.

[0031] The Cistanche deserticola of the invention comes from Xinjiang and is selected from high-quality Cistanche deserticola that is over 5 years old.

[0032] The preparation method of the deer blood extract of the present invention comprises the following steps: mixing sika deer antler blood and 10wt% citric acid solution in a mass ratio of 1:5, shaking at 10°C for 10 minutes, adding a complex enzyme of trypsin, lipase and Bacillus pumilus protease in a mass ratio of 3:1:2, and the mass ratio of the complex enzyme to sika deer antler blood is 0.2:1, performing enzymatic hydrolysis at 30°C for 2 hours, followed by ultrasonic treatment at 15kHz for 20 minutes, centrifuging at 3500rpm for 10 minutes and at 5000rpm for 6 minutes, collecting the supernatant, purifying through an ultrafiltration membrane with a diameter of less than 1kDa, and freeze-drying the filtrate to obtain the deer blood extract.

[0033] The preparation method of the polygonatum extract of the present invention comprises the following steps: crushing polygonatum, adding the mixture into water at a material-liquid ratio of 1:10 g / mL, adding a complex enzyme consisting of xylanase, β-glucanase and hemicellulase in a mass ratio of 1:0.8:1, and the mass ratio of the complex enzyme to polygonatum is 0.15:1, performing enzymolysis at 30°C for 1 hour, centrifuging to obtain an enzymolysis solution and a residue, fermenting the residue at 28°C for 12 hours with an activated composite strain, wherein the composite strain consists of Aspergillus niger, Trichosporon berberis and Saccharomyces cerevisiae in a mass ratio of 3:1:1, and the inoculation amount is 1% of the mass of the residue, to obtain a fermentation product, combining the fermentation product and the enzymolysis solution, adding ethylenediaminetetraacetic acid and ascorbic acid, and oscillating for 10 minutes to obtain a mixed solution, wherein the concentration of ethylenediaminetetraacetic acid in the mixed solution is 3 mmol / L, and the concentration of ascorbic acid is 4 mmol / L, centrifuging, and freeze-drying to obtain the polygonatum extract.

[0034] The preparation method of the Ficus pumila extract of the present invention comprises the following steps: crushing the root or fruit of the Ficus pumila, placing the Ficus pumila root in a supercritical CO2 extraction device, extracting the Ficus pumila root at 30 MPa and 50° C. for 2 h, and concentrating the extract under reduced pressure to obtain the Ficus pumila extract.

[0035] The preparation method of the berry root extract of the present invention comprises the following steps: crushing the berry root, adding the berry root to a 70% v / v ethanol solution at a material-liquid ratio of 1:12 g / mL, performing microwave heating extraction at 400 W and 70° C. for 40 minutes, and concentrating the extract under reduced pressure to obtain the berry root extract.

[0036] Example 1 A deer blood and polygonatum wine for tonifying the kidney and replenishing qi and enhancing immunity comprises the following raw materials, in parts by weight: 55 parts of deer blood extract, 55 parts of polygonatum extract, 35 parts of ficus pumila extract, 15 parts of berry root extract, 7 parts of oyster, 8 parts of eucommia ulmoides leaves, 8 parts of cistanche deserticola, and 500 parts of 50-proof liquor.

[0037] The preparation method of the above-mentioned deer blood and polygonatum wine specifically comprises the following steps: soaking deer blood extract, polygonatum extract, ficus pumila extract, berry root extract, oyster, eucommia leaf and cistanche deserticola in 50% liquor, sealing and storing for 4 months, filtering to obtain the deer blood and polygonatum wine.

[0038] Example 2 A deer blood and polygonatum wine for tonifying the kidney and replenishing qi and enhancing immunity comprises the following raw materials, in parts by weight: 50 parts of deer blood extract, 50 parts of polygonatum extract, 30 parts of ficus pumila extract, 10 parts of berry root extract, 6 parts of oyster, 5 parts of eucommia ulmoides leaves, 5 parts of cistanche deserticola, and 450 parts of 50-proof liquor.

[0039] The preparation method of the above-mentioned deer blood and polygonatum wine specifically comprises the following steps: soaking deer blood extract, polygonatum extract, ficus pumila extract, berry root extract, oyster, eucommia leaf and cistanche deserticola in 50% liquor, sealing and storing for 4 months, filtering to obtain the deer blood and polygonatum wine.

[0040] Example 3 A deer blood and polygonatum wine for tonifying the kidney and replenishing qi and enhancing immunity comprises the following raw materials, in parts by weight: 60 parts of deer blood extract, 60 parts of polygonatum extract, 40 parts of ficus pumila extract, 20 parts of berry root extract, 8 parts of oysters, 10 parts of eucommia leaves, 10 parts of cistanche deserticola, and 550 parts of 50-proof liquor.

[0041] The preparation method of the above-mentioned deer blood and polygonatum wine specifically comprises the following steps: soaking deer blood extract, polygonatum extract, ficus pumila extract, berry root extract, oyster, eucommia leaf and cistanche deserticola in 50% liquor, sealing and storing for 4 months, filtering to obtain the deer blood and polygonatum wine.

[0042] Comparative Example 1 The difference from Example 1 is that the Ficus pumila extract is missing, and the amount of the missing substance is distributed according to the ratio of other active ingredients. Other aspects are the same as Example 1.

[0043] Comparative Example 2 The difference from Example 1 is that the berry root extract is missing, and the amount of the missing substance is distributed according to the ratio of other active ingredients. Other aspects are the same as Example 1.

[0044] Comparative Example 3 The difference from Example 1 is that all the extracts were extracted using ordinary alcohol extraction, and the rest were the same as in Example 1.

[0045] Test Example 1 The sensory quality of the deer blood and polygonatum wine prepared in Examples 1-3 and Comparative Examples 1-3 was evaluated.

[0046] An evaluation team consisting of 20 people (10 women and 10 men) who have received professional sensory training scored the five indicators of Deer Blood and Polygonatum Wine, namely color (20 points), shape (20 points), aroma (20 points), flavor (20 points), and taste (20 points). The total score is 100 points, and the results are averaged.

[0047] The sensory scoring criteria are shown in Table 1; The scoring results are shown in Table 2.

[0048] Table 1

[0049] Table 2

[0050] As can be seen from Table 2, the deer blood and polygonatum wines prepared in Examples 1-3 of the present invention have good indicators in terms of color, shape, aroma, flavor and taste, and are deeply loved by the general consumers.

[0051] Test Example 2 The deer blood and polygonatum wine prepared in Examples 1-3 and Comparative Examples 1-3 were tested for their kidney-tonifying effects and clinical trials were conducted.

[0052] Specific experimental method: Symptoms of kidney deficiency are mostly fatigue, weakness, soreness of waist and knees, cold limbs, loss of libido, lack of energy, senility, etc. 60 cases with the above symptoms were selected, including 30 people aged 18-25 and 30 people aged 26-45. They were randomly divided into 6 groups, each with 10 people. Each group took the deer blood and polygonatum wine prepared in Examples 1-3 and Comparative Examples 1-3, 50 ml each time, twice a day for 2 consecutive months. Hemoglobin, adrenocortical hormone, and lipid peroxide in the blood routine were measured before and after taking the wine. The test results are shown in Table 3.

[0053] Table 3

[0054] As can be seen from Table 3, the deer blood and polygonatum wines prepared in Examples 1-3 of the present invention have a kidney-tonifying effect. After taking the deer blood and polygonatum wines prepared in Examples 1-3, patients' hemoglobin levels significantly increased, indicating that their fatigue, poor spirits, and physical weakness were significantly improved. Their adrenocortical hormone levels increased, indicating that the excitability of the central nervous system of patients with kidney deficiency was significantly improved, which can improve their mental state. In addition, their lipid peroxide levels increased, indicating that the patients' attention was improved.

[0055] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A deer blood and polygonatum wine for tonifying the kidney and replenishing qi and enhancing immunity, characterized in that: The invention comprises the following raw materials in parts by weight: 50-60 parts of deer blood extract, 50-60 parts of polygonatum extract, 30-40 parts of ficus pumila extract, 10-20 parts of berry root extract, 6-8 parts of oyster, 5-10 parts of eucommia leaves, 5-10 parts of cistanche deserticola, and 450-550 parts of 50-proof liquor.

2. The deer blood and polygonatum wine for invigorating the kidney and replenishing qi and enhancing immunity according to claim 1, characterized in that: The preparation method of the deer blood extract comprises: The sika deer antler blood is placed in a citric acid solution and shaken, and a complex enzyme is added for enzymatic hydrolysis, followed by ultrasonic treatment and centrifugation. The supernatant is collected and purified through an ultrafiltration membrane with a diameter of less than 1 kDa. The filtrate is freeze-dried to obtain a deer blood extract.

3. The deer blood and polygonatum wine for tonifying the kidney and replenishing qi and enhancing immunity according to claim 2, characterized in that: The mass ratio of the sika deer antler blood to the citric acid solution is 1:4-6; the concentration of the citric acid solution is 8-12 wt %; the oscillation temperature is 5-10° C., and the oscillation time is 10-15 min; The mass ratio of the complex enzyme to sika deer antler blood is 0.2-0.4:1; the complex enzyme comprises trypsin, lipase and Bacillus pumilus protease in a mass ratio of 3-5:1:2-4; the enzymatic hydrolysis temperature is 28-35°C and the time is 1-3h; The power of the ultrasonic treatment is 10-20 kHz, and the time is 10-20 min; the centrifugation is performed at 2500-3500 rpm for 5-10 min, and at 4000-5000 rpm for 6-8 min.

4. The deer blood and polygonatum wine for tonifying the kidney and replenishing qi and enhancing immunity according to claim 1, characterized in that: The preparation method of the polygonatum extract comprises: The polygonatum sibiricum is crushed and added into water, a composite enzyme is added for enzymolysis, and the mixture is centrifuged to obtain an enzymolysis solution and a residue. The residue is fermented with an activated composite bacterial strain to obtain a fermentation product, the fermentation product and the enzymolysis solution are combined, ethylenediaminetetraacetic acid and ascorbic acid are added for oscillation to obtain a mixed solution, the mixture is centrifuged, and the mixture is freeze-dried to obtain a polygonatum sibiricum extract.

5. The deer blood and polygonatum wine for tonifying the kidney and replenishing qi and enhancing immunity according to claim 4, characterized in that: The material-liquid ratio of polygonatum to water is 1:10-20 g / mL; the mass ratio of the complex enzyme to polygonatum is 0.15-0.3:1; the complex enzyme is xylanase, β-glucanase and hemicellulase in a mass ratio of 1-2:0.8-1.2:1; the enzymatic hydrolysis is carried out at 30-35° C. for 1-2 hours; The inoculation amount of the composite strain is 1%-3% of the mass of the residue; the composite strain is composed of Aspergillus niger, Trichosporon berberis, and Saccharomyces cerevisiae in a mass ratio of 3-4:1:1-2; the fermentation temperature is 28-32°C, and the fermentation time is 10-12 hours; The concentration of EDTA in the mixed solution is 2-3 mmol / L, and the concentration of ascorbic acid is 3-4 mmol / L; the oscillation time is 5-10 min.

6. The deer blood and polygonatum wine for invigorating the kidney and replenishing qi and enhancing immunity according to claim 1, characterized in that: The preparation method of the Ficus pumila extract comprises: The roots or fruits of Ficus pumila are crushed, placed in a supercritical CO2 extraction device for extraction, and the extract is concentrated under reduced pressure to obtain the Ficus pumila extract.

7. The deer blood and polygonatum wine for tonifying the kidney and replenishing qi and enhancing immunity according to claim 6, characterized in that: The supercritical CO2 extraction is performed at a pressure of 20-30 MPa, a temperature of 40-50° C., and a time of 1-2 h.

8. The deer blood and polygonatum wine for tonifying the kidney and replenishing qi and enhancing immunity according to claim 1, characterized in that: The preparation method of the winterberry root extract comprises: The root of the winterberry is crushed, added into an ethanol solution, subjected to microwave heating extraction, and the extract is concentrated under reduced pressure to obtain the winterberry root extract.

9. The deer blood and polygonatum wine for tonifying the kidney and replenishing qi and enhancing immunity according to claim 8, characterized in that: The material-liquid ratio of the winterberry root to the ethanol solution is 1:8-12 g / mL; the concentration of the ethanol solution is 60-70% v / v; the power of the microwave heating extraction is 300-500 W, the temperature is 60-70° C., and the time is 40-60 min.

10. The method for preparing the deer blood and polygonatum wine for invigorating the kidney and replenishing qi and enhancing immunity according to claim 1, characterized in that: The specific preparation steps include: soaking deer blood extract, polygonatum extract, ficus pumila extract, berry root extract, oyster, eucommia leaf and cistanche deserticola in 50-proof liquor, sealing and storing for 3-4 months, filtering and obtaining the target deer blood and polygonatum liquor.

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